Organoids possess immense potential for unraveling the intricate functions of human tissues and facilitating preclinical disease treatment.Their applications span from high-throughput drug screening to the modeling of...Organoids possess immense potential for unraveling the intricate functions of human tissues and facilitating preclinical disease treatment.Their applications span from high-throughput drug screening to the modeling of complex diseases,with some even achieving clinical translation.Changes in the overall size,shape,boundary,and other morphological features of organoids provide a noninvasive method for assessing organoid drug sensitivity.However,the precise segmentation of organoids in bright-field microscopy images is made difficult by the complexity of the organoid morphology and interference,including overlapping organoids,bubbles,dust particles,and cell fragments.This paper introduces the precision organoid segmentation technique(POST),which is a deep-learning algorithm for segmenting challenging organoids under simple bright-field imaging conditions.Unlike existing methods,POST accurately segments each organoid and eliminates various artifacts encountered during organoid culturing and imaging.Furthermore,it is sensitive to and aligns with measurements of organoid activity in drug sensitivity experiments.POST is expected to be a valuable tool for drug screening using organoids owing to its capability of automatically and rapidly eliminating interfering substances and thereby streamlining the organoid analysis and drug screening process.展开更多
3D laser scanning technology is widely used in underground openings for high-precision,rapid,and nondestructive structural evaluations.Segmenting large 3D point cloud datasets,particularly in coal mine roadways with m...3D laser scanning technology is widely used in underground openings for high-precision,rapid,and nondestructive structural evaluations.Segmenting large 3D point cloud datasets,particularly in coal mine roadways with multi-scale targets,remains challenging.This paper proposes an enhanced segmentation method integrating improved PointNet++with a coverage-voted strategy.The coverage-voted strategy reduces data while preserving multi-scale target topology.The segmentation is achieved using an enhanced PointNet++algorithm with a normalization preprocessing head,resulting in a 94%accuracy for common supporting components.Ablation experiments show that the preprocessing head and coverage strategies increase segmentation accuracy by 20%and 2%,respectively,and improve Intersection over Union(IoU)for bearing plate segmentation by 58%and 20%.The accuracy of the current pretraining segmentation model may be affected by variations in surface support components,but it can be readily enhanced through re-optimization with additional labeled point cloud data.This proposed method,combined with a previously developed machine learning model that links rock bolt load and the deformation field of its bearing plate,provides a robust technique for simultaneously measuring the load of multiple rock bolts in a single laser scan.展开更多
With the continuous development of artificial intelligence and machine learning techniques,there have been effective methods supporting the work of dermatologist in the field of skin cancer detection.However,object si...With the continuous development of artificial intelligence and machine learning techniques,there have been effective methods supporting the work of dermatologist in the field of skin cancer detection.However,object significant challenges have been presented in accurately segmenting melanomas in dermoscopic images due to the objects that could interfere human observations,such as bubbles and scales.To address these challenges,we propose a dual U-Net network framework for skin melanoma segmentation.In our proposed architecture,we introduce several innovative components that aim to enhance the performance and capabilities of the traditional U-Net.First,we establish a novel framework that links two simplified U-Nets,enabling more comprehensive information exchange and feature integration throughout the network.Second,after cascading the second U-Net,we introduce a skip connection between the decoder and encoder networks,and incorporate a modified receptive field block(MRFB),which is designed to capture multi-scale spatial information.Third,to further enhance the feature representation capabilities,we add a multi-path convolution block attention module(MCBAM)to the first two layers of the first U-Net encoding,and integrate a new squeeze-and-excitation(SE)mechanism with residual connections in the second U-Net.To illustrate the performance of our proposed model,we conducted comprehensive experiments on widely recognized skin datasets.On the ISIC-2017 dataset,the IoU value of our proposed model increased from 0.6406 to 0.6819 and the Dice coefficient increased from 0.7625 to 0.8023.On the ISIC-2018 dataset,the IoU value of proposed model also improved from 0.7138 to 0.7709,while the Dice coefficient increased from 0.8285 to 0.8665.Furthermore,the generalization experiments conducted on the jaw cyst dataset from Quzhou People’s Hospital further verified the outstanding segmentation performance of the proposed model.These findings collectively affirm the potential of our approach as a valuable tool in supporting clinical decision-making in the field of skin cancer detection,as well as advancing research in medical image analysis.展开更多
This study addresses the persistent scarcity of systematic and comparable data on mountain tourism,with particular reference to Northern Italy,as highlighted by FAO/UNWTO reports and recent academic literature.It aims...This study addresses the persistent scarcity of systematic and comparable data on mountain tourism,with particular reference to Northern Italy,as highlighted by FAO/UNWTO reports and recent academic literature.It aims to contribute to this gap by analyzing tourist flows,socio-demographic characteristics,preferences,and behaviors of domestic visitors to the Italian Alps.Data were collected through a survey conducted between December 2023 and January 2024 among 1,218 residents of Northwest and Northeast Italy and Friuli Venezia Giulia,using a stratified sampling approach.Descriptive statistics and inferential analyses were employed to examine visitation patterns,while K-means clustering was applied to identify distinct segments of mountain tourists based on activity preferences and motivations.Overall,82.5%of respondents reported visiting Alpine areas.Chi-square tests revealed statistically significant differences in visitation behavior according to age,occupational status,and income.Notably,spiritual activities,such as pilgrimages,elicited levels of interest comparable to those of more traditional mountain sports.The cluster analysis identified three visitor profiles:Active Young Enthusiasts,characterized by high engagement in multiple outdoor activities and motivated by psychological well-being and cultural enrichment;Well-being-Oriented Walkers,preferring low-intensity activities primarily driven by psychological relaxation;and Hiking-Oriented Explorers,exhibiting a strong propensity for mountain excursions associated with high levels of psychophysical well-being.These findings enhance understanding of the heterogeneous structure of mountain tourism demand in Northern Italy and offer insights relevant to sustainable destination planning and management in Alpine regions.展开更多
Detailed individual tree crown segmentation is highly relevant for the detection and monitoring of Fraxinus excelsior L.trees affected by ash dieback,a major threat to common ash populations across Europe.In this stud...Detailed individual tree crown segmentation is highly relevant for the detection and monitoring of Fraxinus excelsior L.trees affected by ash dieback,a major threat to common ash populations across Europe.In this study,both fine and coarse crown segmentation methods were applied to close-range multispectral UAV imagery.The fine tree crown segmentation method utilized a novel unsupervised machine learning approach based on a blended NIR-NDVI image,whereas the coarse segmentation relied on the segment anything model(SAM).Both methods successfully delineated tree crown outlines,however,only the fine segmentation accurately captured internal canopy gaps.Despite these structural differences,mean NDVI values calculated per tree crown revealed no significant differences between the two approaches,indicating that coarse segmentation is sufficient for mean vegetation index assessments.Nevertheless,the fine segmentation revealed increased heterogeneity in NDVI values in more severely damaged trees,underscoring its value for detailed structural and health analyses.Furthermore,the fine segmentation workflow proved transferable to both individual UAV images and orthophotos from broader UAV surveys.For applications focused on structural integrity and spatial variation in canopy health,the fine segmentation approach is recommended.展开更多
AIM:To construct an intelligent segmentation scheme for precise localization of central serous chorioretinopathy(CSC)leakage points,thereby enabling ophthalmologists to deliver accurate laser treatment without navigat...AIM:To construct an intelligent segmentation scheme for precise localization of central serous chorioretinopathy(CSC)leakage points,thereby enabling ophthalmologists to deliver accurate laser treatment without navigational laser equipment.METHODS:A dataset with dual labels(point-level and pixel-level)was first established based on fundus fluorescein angiography(FFA)images of CSC and subsequently divided into training(102 images),validation(40 images),and test(40 images)datasets.An intelligent segmentation method was then developed,based on the You Only Look Once version 8 Pose Estimation(YOLOv8-Pose)model and segment anything model(SAM),to segment CSC leakage points.Next,the YOLOv8-Pose model was trained for 200 epochs,and the best-performing model was selected to form the optimal combination with SAM.Additionally,the classic five types of U-Net series models[i.e.,U-Net,recurrent residual U-Net(R2U-Net),attention U-Net(AttU-Net),recurrent residual attention U-Net(R2AttUNet),and nested U-Net(UNet^(++))]were initialized with three random seeds and trained for 200 epochs,resulting in a total of 15 baseline models for comparison.Finally,based on the metrics including Dice similarity coefficient(DICE),intersection over union(IoU),precision,recall,precisionrecall(PR)curve,and receiver operating characteristic(ROC)curve,the proposed method was compared with baseline models through quantitative and qualitative experiments for leakage point segmentation,thereby demonstrating its effectiveness.RESULTS:With the increase of training epochs,the mAP50-95,Recall,and precision of the YOLOv8-Pose model showed a significant increase and tended to stabilize,and it achieved a preliminary localization success rate of 90%(i.e.,36 images)for CSC leakage points in 40 test images.Using manually expert-annotated pixel-level labels as the ground truth,the proposed method achieved outcomes with a DICE of 57.13%,an IoU of 45.31%,a precision of 45.91%,a recall of 93.57%,an area under the PR curve(AUC-PR)of 0.78 and an area under the ROC curve(AUC-ROC)of 0.97,which enables more accurate segmentation of CSC leakage points.CONCLUSION:By combining the precise localization capability of the YOLOv8-Pose model with the robust and flexible segmentation ability of SAM,the proposed method not only demonstrates the effectiveness of the YOLOv8-Pose model in detecting keypoint coordinates of CSC leakage points from the perspective of application innovation but also establishes a novel approach for accurate segmentation of CSC leakage points through the“detect-then-segment”strategy,thereby providing a potential auxiliary means for the automatic and precise realtime localization of leakage points during traditional laser photocoagulation for CSC.展开更多
Medical image segmentation is of critical importance in the domain of contemporary medical imaging.However,U-Net and its variants exhibit limitations in capturing complex nonlinear patterns and global contextual infor...Medical image segmentation is of critical importance in the domain of contemporary medical imaging.However,U-Net and its variants exhibit limitations in capturing complex nonlinear patterns and global contextual information.Although the subsequent U-KAN model enhances nonlinear representation capabilities,it still faces challenges such as gradient vanishing during deep network training and spatial detail loss during feature downsampling,resulting in insufficient segmentation accuracy for edge structures and minute lesions.To address these challenges,this paper proposes the RE-UKAN model,which innovatively improves upon U-KAN.Firstly,a residual network is introduced into the encoder to effectively mitigate gradient vanishing through cross-layer identity mappings,thus enhancing modelling capabilities for complex pathological structures.Secondly,Efficient Local Attention(ELA)is integrated to suppress spatial detail loss during downsampling,thereby improving the perception of edge structures and minute lesions.Experimental results on four public datasets demonstrate that RE-UKAN outperforms existing medical image segmentation methods across multiple evaluation metrics,with particularly outstanding performance on the TN-SCUI 2020 dataset,achieving IoU of 88.18%and Dice of 93.57%.Compared to the baseline model,it achieves improvements of 3.05%and 1.72%,respectively.These results fully demonstrate RE-UKAN’s superior detail retention capability and boundary recognition accuracy in complex medical image segmentation tasks,providing a reliable solution for clinical precision segmentation.展开更多
Bilayer MoS2 is a promising channel candidate for extending Moore’s law,owing to its optimal channel thickness and improved suppression of extrinsic scattering compared to monolayers.However,its intrinsic phonon-limi...Bilayer MoS2 is a promising channel candidate for extending Moore’s law,owing to its optimal channel thickness and improved suppression of extrinsic scattering compared to monolayers.However,its intrinsic phonon-limited electron mobility is severely limited by enhanced K–Q intervalley scattering arising from the multivalley conduction band feature inherent to the bilayer structure.To overcome this bottleneck,we propose a“valley separation engineering”strategy that combines a twist angle near 30°with applied stress.Our first-principles calculations demonstrate that although valley separation can be continuously increased using this strategy,the electron mobility saturates at∼200 cm^(2)⋅V^(−1)⋅s^(−1).The saturation is attributed to the competition between the reduced effective mass and enhanced intravalley scattering induced by phonon softening once the detrimental intervalley scattering is effectively suppressed by sufficient valley separation.This study establishes a theoretical upper limit for the intrinsic electron transport of bilayer MoS2 masked by severe intervalley scattering.展开更多
Weakly Supervised Semantic Segmentation(WSSS),which relies only on image-level labels,has attracted significant attention for its cost-effectiveness and scalability.Existing methods mainly enhance inter-class distinct...Weakly Supervised Semantic Segmentation(WSSS),which relies only on image-level labels,has attracted significant attention for its cost-effectiveness and scalability.Existing methods mainly enhance inter-class distinctions and employ data augmentation to mitigate semantic ambiguity and reduce spurious activations.However,they often neglect the complex contextual dependencies among image patches,resulting in incomplete local representations and limited segmentation accuracy.To address these issues,we propose the Context Patch Fusion with Class Token Enhancement(CPF-CTE)framework,which exploits contextual relations among patches to enrich feature repre-sentations and improve segmentation.At its core,the Contextual-Fusion Bidirectional Long Short-Term Memory(CF-BiLSTM)module captures spatial dependencies between patches and enables bidirectional information flow,yield-ing a more comprehensive understanding of spatial correlations.This strengthens feature learning and segmentation robustness.Moreover,we introduce learnable class tokens that dynamically encode and refine class-specific semantics,enhancing discriminative capability.By effectively integrating spatial and semantic cues,CPF-CTE produces richer and more accurate representations of image content.Extensive experiments on PASCAL VOC 2012 and MS COCO 2014 validate that CPF-CTE consistently surpasses prior WSSS methods.展开更多
Dear Editor,This letter presents techniques to simplify dataset generation for instance segmentation of raw meat products,a critical step toward automating food production lines.Accurate segmentation is essential for ...Dear Editor,This letter presents techniques to simplify dataset generation for instance segmentation of raw meat products,a critical step toward automating food production lines.Accurate segmentation is essential for addressing challenges such as occlusions,indistinct edges,and stacked configurations,which demand large,diverse datasets.To meet these demands,we propose two complementary approaches:a semi-automatic annotation interface using tools like the segment anything model(SAM)and GrabCut and a synthetic data generation pipeline leveraging 3D-scanned models.These methods reduce reliance on real meat,mitigate food waste,and improve scalability.Experimental results demonstrate that incorporating synthetic data enhances segmentation model performance and,when combined with real data,further boosts accuracy,paving the way for more efficient automation in the food industry.展开更多
Quantitative analysis of aluminum-silicon(Al-Si)alloy microstructure is crucial for evaluating and controlling alloy performance.Conventional analysis methods rely on manual segmentation,which is inefficient and subje...Quantitative analysis of aluminum-silicon(Al-Si)alloy microstructure is crucial for evaluating and controlling alloy performance.Conventional analysis methods rely on manual segmentation,which is inefficient and subjective,while fully supervised deep learning approaches require extensive and expensive pixel-level annotated data.Furthermore,existing semi-supervised methods still face challenges in handling the adhesion of adjacent primary silicon particles and effectively utilizing consistency in unlabeled data.To address these issues,this paper proposes a novel semi-supervised framework for Al-Si alloy microstructure image segmentation.First,we introduce a Rotational Uncertainty Correction Strategy(RUCS).This strategy employs multi-angle rotational perturbations andMonte Carlo sampling to assess prediction consistency,generating a pixel-wise confidence weight map.By integrating this map into the loss function,the model dynamically focuses on high-confidence regions,thereby improving generalization ability while reducing manual annotation pressure.Second,we design a Boundary EnhancementModule(BEM)to strengthen boundary feature extraction through erosion difference and multi-scale dilated convolutions.This module guides the model to focus on the boundary regions of adjacent particles,effectively resolving particle adhesion and improving segmentation accuracy.Systematic experiments were conducted on the Aluminum-Silicon Alloy Microstructure Dataset(ASAD).Results indicate that the proposed method performs exceptionally well with scarce labeled data.Specifically,using only 5%labeled data,our method improves the Jaccard index and Adjusted Rand Index(ARI)by 2.84 and 1.57 percentage points,respectively,and reduces the Variation of Information(VI)by 8.65 compared to stateof-the-art semi-supervised models,approaching the performance levels of 10%labeled data.These results demonstrate that the proposed method significantly enhances the accuracy and robustness of quantitative microstructure analysis while reducing annotation costs.展开更多
This article studies the problem of image segmentation-based semantic communication in autonomous driving.In real traffic scenes,the detecting of objects(e.g.,vehicles and pedestrians)is more important to guarantee dr...This article studies the problem of image segmentation-based semantic communication in autonomous driving.In real traffic scenes,the detecting of objects(e.g.,vehicles and pedestrians)is more important to guarantee driving safety,which is always ignored in existing works.Therefore,we propose a vehicular image segmentation-oriented semantic communication system,termed VIS-SemCom,focusing on transmitting and recovering image semantic features of high-important objects to reduce transmission redundancy.First,we develop a semantic codec based on Swin Transformer architecture,which expands the perceptual field thus improving the segmentation accuracy.To highlight the important objects'accuracy,we propose a multi-scale semantic extraction method by assigning the number of Swin Transformer blocks for diverse resolution semantic features.Also,an importance-aware loss incorporating important levels is devised,and an online hard example mining(OHEM)strategy is proposed to handle small sample issues in the dataset.Finally,experimental results demonstrate that the proposed VIS-SemCom can achieve a significant mean intersection over union(mIoU)performance in the SNR regions,a reduction of transmitted data volume by about 60%at 60%mIoU,and improve the segmentation accuracy of important objects,compared to baseline image communication.展开更多
Magnetic Resonance Imaging(MRI)has a pivotal role in medical image analysis,for its ability in supporting disease detection and diagnosis.Fuzzy C-Means(FCM)clustering is widely used for MRI segmentation due to its abi...Magnetic Resonance Imaging(MRI)has a pivotal role in medical image analysis,for its ability in supporting disease detection and diagnosis.Fuzzy C-Means(FCM)clustering is widely used for MRI segmentation due to its ability to handle image uncertainty.However,the latter still has countless limitations,including sensitivity to initialization,susceptibility to local optima,and high computational cost.To address these limitations,this study integrates Grey Wolf Optimization(GWO)with FCM to enhance cluster center selection,improving segmentation accuracy and robustness.Moreover,to further refine optimization,Fuzzy Entropy Clustering was utilized for its distinctive features from other traditional objective functions.Fuzzy entropy effectively quantifies uncertainty,leading to more well-defined clusters,improved noise robustness,and better preservation of anatomical structures in MRI images.Despite these advantages,the iterative nature of GWO and FCM introduces significant computational overhead,which restricts their applicability to high-resolution medical images.To overcome this bottleneck,we propose a Parallelized-GWO-based FCM(P-GWO-FCM)approach using GPU acceleration,where both GWO optimization and FCM updates(centroid computation and membership matrix updates)are parallelized.By concurrently executing these processes,our approach efficiently distributes the computational workload,significantly reducing execution time while maintaining high segmentation accuracy.The proposed parallel method,P-GWO-FCM,was evaluated on both simulated and clinical brain MR images,focusing on segmenting white matter,gray matter,and cerebrospinal fluid regions.The results indicate significant improvements in segmentation accuracy,achieving a Jaccard Similarity(JS)of 0.92,a Partition Coefficient Index(PCI)of 0.91,a Partition Entropy Index(PEI)of 0.25,and a Davies-Bouldin Index(DBI)of 0.30.Experimental comparisons demonstrate that P-GWO-FCM outperforms existing methods in both segmentation accuracy and computational efficiency,making it a promising solution for real-time medical image segmentation.展开更多
Autonomous vehicles rely heavily on accurate and efficient scene segmentation for safe navigation and efficient operations.Traditional Bird’s Eye View(BEV)methods on semantic scene segmentation,which leverage multimo...Autonomous vehicles rely heavily on accurate and efficient scene segmentation for safe navigation and efficient operations.Traditional Bird’s Eye View(BEV)methods on semantic scene segmentation,which leverage multimodal sensor fusion,often struggle with noisy data and demand high-performance GPUs,leading to sensor misalignment and performance degradation.This paper introduces an Enhanced Channel Attention BEV(ECABEV),a novel approach designed to address the challenges under insufficient GPU memory conditions.ECABEV integrates camera and radar data through a de-noise enhanced channel attention mechanism,which utilizes global average and max pooling to effectively filter out noise while preserving discriminative features.Furthermore,an improved fusion approach is proposed to efficiently merge categorical data across modalities.To reduce computational overhead,a bilinear interpolation layer normalizationmethod is devised to ensure spatial feature fidelity.Moreover,a scalable crossentropy loss function is further designed to handle the imbalanced classes with less computational efficiency sacrifice.Extensive experiments on the nuScenes dataset demonstrate that ECABEV achieves state-of-the-art performance with an IoU of 39.961,using a lightweight ViT-B/14 backbone and lower resolution(224×224).Our approach highlights its cost-effectiveness and practical applicability,even on low-end devices.The code is publicly available at:https://github.com/YYF-CQU/ECABEV.git.展开更多
This study aimed to enhance the performance of semantic segmentation for autonomous driving by improving the 2DPASS model.Two novel improvements were proposed and implemented in this paper:dynamically adjusting the lo...This study aimed to enhance the performance of semantic segmentation for autonomous driving by improving the 2DPASS model.Two novel improvements were proposed and implemented in this paper:dynamically adjusting the loss function ratio and integrating an attention mechanism(CBAM).First,the loss function weights were adjusted dynamically.The grid search method is used for deciding the best ratio of 7:3.It gives greater emphasis to the cross-entropy loss,which resulted in better segmentation performance.Second,CBAM was applied at different layers of the 2Dencoder.Heatmap analysis revealed that introducing it after the second block of 2D image encoding produced the most effective enhancement of important feature representation.The training epoch was chosen for optimizing the best value by experiments,which improved model convergence and overall accuracy.To evaluate the proposed approach,experiments were conducted based on the SemanticKITTI database.The results showed that the improved model achieved higher segmentation accuracy by 64.31%,improved 11.47% in mIoU compared with the conventional 2DPASS model(baseline:52.84%).It was more effective at detecting small and distant objects and clearly identifying boundaries between different classes.Issues such as noise and variations in data distribution affected its accuracy,indicating the need for further refinement.Overall,the proposed improvements to the 2DPASS model demonstrated the potential to advance semantic segmentation technology and contributed to a more reliable perception of complex,dynamic environments in autonomous vehicles.Accurate segmentation enhances the vehicle’s ability to distinguish different objects,and this improvement directly supports safer navigation,robust decision-making,and efficient path planning,making it highly applicable to real-world deployment of autonomous systems in urban and highway settings.展开更多
The rising need for precision farming and sustainable land management has catalyzed the requirement for sophisticated means of deriving practical data from remote sensing images.Image segmentation,or the process of di...The rising need for precision farming and sustainable land management has catalyzed the requirement for sophisticated means of deriving practical data from remote sensing images.Image segmentation,or the process of dividing the image into semantically relevant parts,has become a groundbreaking technology that allows resolving the problem of transitioning the pixel-level data to a parcel-level analysis.This review is a synthesis of the segmentation methods and their use in crop research and geospatial science.The architectures of pixel-based,object-based,and deep learning(convolutional neural networks,U-Net,Mask R-CNN,and Transformer models)are considered in terms of principles,capabilities,and limitations.Multi-spectral,hyperspectral,LiDAR,and SAR data are integrated to improve the efficiency of segmentation,allowing the possible delineation of fields,the classification of crops,health monitoring,monitoring of yields,and stress identification.In addition to agriculture,segmentation helps in land use and land cover mapping,identification of temporal change,monitoring of the environment,and is used in combination with GIS-based spatial modeling.Nevertheless,issues related to data heterogeneity,mixed pixels,computational requirements,and inadequate availability of labelled data still exist despite the major progress.The future directions involve multi-source data fusion,pixel-to-parcel pipeline automation,and predictive models based on AI,which are used to enhance its scalability,robustness,and the ability to monitor in real-time.This review makes it clear that the use of image segmentation as a tool in generating precision agriculture,sustainable land use,and informed geospatial.展开更多
基金supported by the National Key R&D Program of China(No.2022YFC2504403)the National Natural Science Foundation of China(No.62172202)+1 种基金the Experiment Project of China Manned Space Program(No.HYZHXM01019)the Fundamental Research Funds for the Central Universities from Southeast University(No.3207032101C3)。
文摘Organoids possess immense potential for unraveling the intricate functions of human tissues and facilitating preclinical disease treatment.Their applications span from high-throughput drug screening to the modeling of complex diseases,with some even achieving clinical translation.Changes in the overall size,shape,boundary,and other morphological features of organoids provide a noninvasive method for assessing organoid drug sensitivity.However,the precise segmentation of organoids in bright-field microscopy images is made difficult by the complexity of the organoid morphology and interference,including overlapping organoids,bubbles,dust particles,and cell fragments.This paper introduces the precision organoid segmentation technique(POST),which is a deep-learning algorithm for segmenting challenging organoids under simple bright-field imaging conditions.Unlike existing methods,POST accurately segments each organoid and eliminates various artifacts encountered during organoid culturing and imaging.Furthermore,it is sensitive to and aligns with measurements of organoid activity in drug sensitivity experiments.POST is expected to be a valuable tool for drug screening using organoids owing to its capability of automatically and rapidly eliminating interfering substances and thereby streamlining the organoid analysis and drug screening process.
基金supported by the National Natural Science Foundation of China(Grant Nos.52304139,52325403)the CCTEG Coal Mining Research Institute funding(Grant No.KCYJY-2024-MS-10).
文摘3D laser scanning technology is widely used in underground openings for high-precision,rapid,and nondestructive structural evaluations.Segmenting large 3D point cloud datasets,particularly in coal mine roadways with multi-scale targets,remains challenging.This paper proposes an enhanced segmentation method integrating improved PointNet++with a coverage-voted strategy.The coverage-voted strategy reduces data while preserving multi-scale target topology.The segmentation is achieved using an enhanced PointNet++algorithm with a normalization preprocessing head,resulting in a 94%accuracy for common supporting components.Ablation experiments show that the preprocessing head and coverage strategies increase segmentation accuracy by 20%and 2%,respectively,and improve Intersection over Union(IoU)for bearing plate segmentation by 58%and 20%.The accuracy of the current pretraining segmentation model may be affected by variations in surface support components,but it can be readily enhanced through re-optimization with additional labeled point cloud data.This proposed method,combined with a previously developed machine learning model that links rock bolt load and the deformation field of its bearing plate,provides a robust technique for simultaneously measuring the load of multiple rock bolts in a single laser scan.
基金funded by Zhejiang Basic Public Welfare Research Project,grant number LZY24E060001supported by Guangzhou Development Zone Science and Technology(2021GH10,2020GH10,2023GH02)+1 种基金the University of Macao(MYRG2022-00271-FST)the Science and Technology Development Fund(FDCT)of Macao(0032/2022/A).
文摘With the continuous development of artificial intelligence and machine learning techniques,there have been effective methods supporting the work of dermatologist in the field of skin cancer detection.However,object significant challenges have been presented in accurately segmenting melanomas in dermoscopic images due to the objects that could interfere human observations,such as bubbles and scales.To address these challenges,we propose a dual U-Net network framework for skin melanoma segmentation.In our proposed architecture,we introduce several innovative components that aim to enhance the performance and capabilities of the traditional U-Net.First,we establish a novel framework that links two simplified U-Nets,enabling more comprehensive information exchange and feature integration throughout the network.Second,after cascading the second U-Net,we introduce a skip connection between the decoder and encoder networks,and incorporate a modified receptive field block(MRFB),which is designed to capture multi-scale spatial information.Third,to further enhance the feature representation capabilities,we add a multi-path convolution block attention module(MCBAM)to the first two layers of the first U-Net encoding,and integrate a new squeeze-and-excitation(SE)mechanism with residual connections in the second U-Net.To illustrate the performance of our proposed model,we conducted comprehensive experiments on widely recognized skin datasets.On the ISIC-2017 dataset,the IoU value of our proposed model increased from 0.6406 to 0.6819 and the Dice coefficient increased from 0.7625 to 0.8023.On the ISIC-2018 dataset,the IoU value of proposed model also improved from 0.7138 to 0.7709,while the Dice coefficient increased from 0.8285 to 0.8665.Furthermore,the generalization experiments conducted on the jaw cyst dataset from Quzhou People’s Hospital further verified the outstanding segmentation performance of the proposed model.These findings collectively affirm the potential of our approach as a valuable tool in supporting clinical decision-making in the field of skin cancer detection,as well as advancing research in medical image analysis.
基金funded by the European Union—Next Generation EU,in the framework of the consortium i NEST—Interconnected Nord-Est Innovation Ecosystem(PNRR,Missione 4 Componente 2,Investimento 1.5 D.D.105823 June 2022,ECS_00000043—Spoke1,RT2,CUP I43C22000250006)。
文摘This study addresses the persistent scarcity of systematic and comparable data on mountain tourism,with particular reference to Northern Italy,as highlighted by FAO/UNWTO reports and recent academic literature.It aims to contribute to this gap by analyzing tourist flows,socio-demographic characteristics,preferences,and behaviors of domestic visitors to the Italian Alps.Data were collected through a survey conducted between December 2023 and January 2024 among 1,218 residents of Northwest and Northeast Italy and Friuli Venezia Giulia,using a stratified sampling approach.Descriptive statistics and inferential analyses were employed to examine visitation patterns,while K-means clustering was applied to identify distinct segments of mountain tourists based on activity preferences and motivations.Overall,82.5%of respondents reported visiting Alpine areas.Chi-square tests revealed statistically significant differences in visitation behavior according to age,occupational status,and income.Notably,spiritual activities,such as pilgrimages,elicited levels of interest comparable to those of more traditional mountain sports.The cluster analysis identified three visitor profiles:Active Young Enthusiasts,characterized by high engagement in multiple outdoor activities and motivated by psychological well-being and cultural enrichment;Well-being-Oriented Walkers,preferring low-intensity activities primarily driven by psychological relaxation;and Hiking-Oriented Explorers,exhibiting a strong propensity for mountain excursions associated with high levels of psychophysical well-being.These findings enhance understanding of the heterogeneous structure of mountain tourism demand in Northern Italy and offer insights relevant to sustainable destination planning and management in Alpine regions.
基金This study was conducted within the project FraxVir“Detection,characterisation and analyses of the occurrence of viruses and ash dieback in special stands of Fraxinus excelsior-a supplementary study to the FraxForFuture demonstration project”and receives funding via the Waldklimafonds(WKF)funded by the German Federal Ministry of Food and Agriculture(BMEL)and Federal Ministry for the Environment,Nature Conservation,Nuclear Safety and Consumer Protection(BMUV)administrated by the Agency for Renewable Resources(FNR)under grant agreement 2220WK40A4.
文摘Detailed individual tree crown segmentation is highly relevant for the detection and monitoring of Fraxinus excelsior L.trees affected by ash dieback,a major threat to common ash populations across Europe.In this study,both fine and coarse crown segmentation methods were applied to close-range multispectral UAV imagery.The fine tree crown segmentation method utilized a novel unsupervised machine learning approach based on a blended NIR-NDVI image,whereas the coarse segmentation relied on the segment anything model(SAM).Both methods successfully delineated tree crown outlines,however,only the fine segmentation accurately captured internal canopy gaps.Despite these structural differences,mean NDVI values calculated per tree crown revealed no significant differences between the two approaches,indicating that coarse segmentation is sufficient for mean vegetation index assessments.Nevertheless,the fine segmentation revealed increased heterogeneity in NDVI values in more severely damaged trees,underscoring its value for detailed structural and health analyses.Furthermore,the fine segmentation workflow proved transferable to both individual UAV images and orthophotos from broader UAV surveys.For applications focused on structural integrity and spatial variation in canopy health,the fine segmentation approach is recommended.
基金Supported by the Shenzhen Science and Technology Program(No.JCYJ20240813152704006)the National Natural Science Foundation of China(No.62401259)+2 种基金the Fundamental Research Funds for the Central Universities(No.NZ2024036)the Postdoctoral Fellowship Program of CPSF(No.GZC20242228)High Performance Computing Platform of Nanjing University of Aeronautics and Astronautics。
文摘AIM:To construct an intelligent segmentation scheme for precise localization of central serous chorioretinopathy(CSC)leakage points,thereby enabling ophthalmologists to deliver accurate laser treatment without navigational laser equipment.METHODS:A dataset with dual labels(point-level and pixel-level)was first established based on fundus fluorescein angiography(FFA)images of CSC and subsequently divided into training(102 images),validation(40 images),and test(40 images)datasets.An intelligent segmentation method was then developed,based on the You Only Look Once version 8 Pose Estimation(YOLOv8-Pose)model and segment anything model(SAM),to segment CSC leakage points.Next,the YOLOv8-Pose model was trained for 200 epochs,and the best-performing model was selected to form the optimal combination with SAM.Additionally,the classic five types of U-Net series models[i.e.,U-Net,recurrent residual U-Net(R2U-Net),attention U-Net(AttU-Net),recurrent residual attention U-Net(R2AttUNet),and nested U-Net(UNet^(++))]were initialized with three random seeds and trained for 200 epochs,resulting in a total of 15 baseline models for comparison.Finally,based on the metrics including Dice similarity coefficient(DICE),intersection over union(IoU),precision,recall,precisionrecall(PR)curve,and receiver operating characteristic(ROC)curve,the proposed method was compared with baseline models through quantitative and qualitative experiments for leakage point segmentation,thereby demonstrating its effectiveness.RESULTS:With the increase of training epochs,the mAP50-95,Recall,and precision of the YOLOv8-Pose model showed a significant increase and tended to stabilize,and it achieved a preliminary localization success rate of 90%(i.e.,36 images)for CSC leakage points in 40 test images.Using manually expert-annotated pixel-level labels as the ground truth,the proposed method achieved outcomes with a DICE of 57.13%,an IoU of 45.31%,a precision of 45.91%,a recall of 93.57%,an area under the PR curve(AUC-PR)of 0.78 and an area under the ROC curve(AUC-ROC)of 0.97,which enables more accurate segmentation of CSC leakage points.CONCLUSION:By combining the precise localization capability of the YOLOv8-Pose model with the robust and flexible segmentation ability of SAM,the proposed method not only demonstrates the effectiveness of the YOLOv8-Pose model in detecting keypoint coordinates of CSC leakage points from the perspective of application innovation but also establishes a novel approach for accurate segmentation of CSC leakage points through the“detect-then-segment”strategy,thereby providing a potential auxiliary means for the automatic and precise realtime localization of leakage points during traditional laser photocoagulation for CSC.
文摘Medical image segmentation is of critical importance in the domain of contemporary medical imaging.However,U-Net and its variants exhibit limitations in capturing complex nonlinear patterns and global contextual information.Although the subsequent U-KAN model enhances nonlinear representation capabilities,it still faces challenges such as gradient vanishing during deep network training and spatial detail loss during feature downsampling,resulting in insufficient segmentation accuracy for edge structures and minute lesions.To address these challenges,this paper proposes the RE-UKAN model,which innovatively improves upon U-KAN.Firstly,a residual network is introduced into the encoder to effectively mitigate gradient vanishing through cross-layer identity mappings,thus enhancing modelling capabilities for complex pathological structures.Secondly,Efficient Local Attention(ELA)is integrated to suppress spatial detail loss during downsampling,thereby improving the perception of edge structures and minute lesions.Experimental results on four public datasets demonstrate that RE-UKAN outperforms existing medical image segmentation methods across multiple evaluation metrics,with particularly outstanding performance on the TN-SCUI 2020 dataset,achieving IoU of 88.18%and Dice of 93.57%.Compared to the baseline model,it achieves improvements of 3.05%and 1.72%,respectively.These results fully demonstrate RE-UKAN’s superior detail retention capability and boundary recognition accuracy in complex medical image segmentation tasks,providing a reliable solution for clinical precision segmentation.
基金supported by the National Key R&D Program of China (Grant No.2022YFA1402503)the High Performance Computing Center of Jilin University,China。
文摘Bilayer MoS2 is a promising channel candidate for extending Moore’s law,owing to its optimal channel thickness and improved suppression of extrinsic scattering compared to monolayers.However,its intrinsic phonon-limited electron mobility is severely limited by enhanced K–Q intervalley scattering arising from the multivalley conduction band feature inherent to the bilayer structure.To overcome this bottleneck,we propose a“valley separation engineering”strategy that combines a twist angle near 30°with applied stress.Our first-principles calculations demonstrate that although valley separation can be continuously increased using this strategy,the electron mobility saturates at∼200 cm^(2)⋅V^(−1)⋅s^(−1).The saturation is attributed to the competition between the reduced effective mass and enhanced intravalley scattering induced by phonon softening once the detrimental intervalley scattering is effectively suppressed by sufficient valley separation.This study establishes a theoretical upper limit for the intrinsic electron transport of bilayer MoS2 masked by severe intervalley scattering.
文摘Weakly Supervised Semantic Segmentation(WSSS),which relies only on image-level labels,has attracted significant attention for its cost-effectiveness and scalability.Existing methods mainly enhance inter-class distinctions and employ data augmentation to mitigate semantic ambiguity and reduce spurious activations.However,they often neglect the complex contextual dependencies among image patches,resulting in incomplete local representations and limited segmentation accuracy.To address these issues,we propose the Context Patch Fusion with Class Token Enhancement(CPF-CTE)framework,which exploits contextual relations among patches to enrich feature repre-sentations and improve segmentation.At its core,the Contextual-Fusion Bidirectional Long Short-Term Memory(CF-BiLSTM)module captures spatial dependencies between patches and enables bidirectional information flow,yield-ing a more comprehensive understanding of spatial correlations.This strengthens feature learning and segmentation robustness.Moreover,we introduce learnable class tokens that dynamically encode and refine class-specific semantics,enhancing discriminative capability.By effectively integrating spatial and semantic cues,CPF-CTE produces richer and more accurate representations of image content.Extensive experiments on PASCAL VOC 2012 and MS COCO 2014 validate that CPF-CTE consistently surpasses prior WSSS methods.
基金supported by European Union’s Horizon Europe research and innovation programme,project AGILEHAND(Smart Grading,Handling and Packaging Solutions for Soft and Deformable Products in Agile and Reconfigurable Lines)(101092043).
文摘Dear Editor,This letter presents techniques to simplify dataset generation for instance segmentation of raw meat products,a critical step toward automating food production lines.Accurate segmentation is essential for addressing challenges such as occlusions,indistinct edges,and stacked configurations,which demand large,diverse datasets.To meet these demands,we propose two complementary approaches:a semi-automatic annotation interface using tools like the segment anything model(SAM)and GrabCut and a synthetic data generation pipeline leveraging 3D-scanned models.These methods reduce reliance on real meat,mitigate food waste,and improve scalability.Experimental results demonstrate that incorporating synthetic data enhances segmentation model performance and,when combined with real data,further boosts accuracy,paving the way for more efficient automation in the food industry.
基金funded by the National Natural Science Foundation of China (52061020).
文摘Quantitative analysis of aluminum-silicon(Al-Si)alloy microstructure is crucial for evaluating and controlling alloy performance.Conventional analysis methods rely on manual segmentation,which is inefficient and subjective,while fully supervised deep learning approaches require extensive and expensive pixel-level annotated data.Furthermore,existing semi-supervised methods still face challenges in handling the adhesion of adjacent primary silicon particles and effectively utilizing consistency in unlabeled data.To address these issues,this paper proposes a novel semi-supervised framework for Al-Si alloy microstructure image segmentation.First,we introduce a Rotational Uncertainty Correction Strategy(RUCS).This strategy employs multi-angle rotational perturbations andMonte Carlo sampling to assess prediction consistency,generating a pixel-wise confidence weight map.By integrating this map into the loss function,the model dynamically focuses on high-confidence regions,thereby improving generalization ability while reducing manual annotation pressure.Second,we design a Boundary EnhancementModule(BEM)to strengthen boundary feature extraction through erosion difference and multi-scale dilated convolutions.This module guides the model to focus on the boundary regions of adjacent particles,effectively resolving particle adhesion and improving segmentation accuracy.Systematic experiments were conducted on the Aluminum-Silicon Alloy Microstructure Dataset(ASAD).Results indicate that the proposed method performs exceptionally well with scarce labeled data.Specifically,using only 5%labeled data,our method improves the Jaccard index and Adjusted Rand Index(ARI)by 2.84 and 1.57 percentage points,respectively,and reduces the Variation of Information(VI)by 8.65 compared to stateof-the-art semi-supervised models,approaching the performance levels of 10%labeled data.These results demonstrate that the proposed method significantly enhances the accuracy and robustness of quantitative microstructure analysis while reducing annotation costs.
基金National Natural Science Foundation of China under Grants No.62171047,U22B2001,62271065,62001051Beijing Natural Science Foundation under Grant L223027BUPT Excellent Ph.D Students Foundation under Grants CX2021114。
文摘This article studies the problem of image segmentation-based semantic communication in autonomous driving.In real traffic scenes,the detecting of objects(e.g.,vehicles and pedestrians)is more important to guarantee driving safety,which is always ignored in existing works.Therefore,we propose a vehicular image segmentation-oriented semantic communication system,termed VIS-SemCom,focusing on transmitting and recovering image semantic features of high-important objects to reduce transmission redundancy.First,we develop a semantic codec based on Swin Transformer architecture,which expands the perceptual field thus improving the segmentation accuracy.To highlight the important objects'accuracy,we propose a multi-scale semantic extraction method by assigning the number of Swin Transformer blocks for diverse resolution semantic features.Also,an importance-aware loss incorporating important levels is devised,and an online hard example mining(OHEM)strategy is proposed to handle small sample issues in the dataset.Finally,experimental results demonstrate that the proposed VIS-SemCom can achieve a significant mean intersection over union(mIoU)performance in the SNR regions,a reduction of transmitted data volume by about 60%at 60%mIoU,and improve the segmentation accuracy of important objects,compared to baseline image communication.
文摘Magnetic Resonance Imaging(MRI)has a pivotal role in medical image analysis,for its ability in supporting disease detection and diagnosis.Fuzzy C-Means(FCM)clustering is widely used for MRI segmentation due to its ability to handle image uncertainty.However,the latter still has countless limitations,including sensitivity to initialization,susceptibility to local optima,and high computational cost.To address these limitations,this study integrates Grey Wolf Optimization(GWO)with FCM to enhance cluster center selection,improving segmentation accuracy and robustness.Moreover,to further refine optimization,Fuzzy Entropy Clustering was utilized for its distinctive features from other traditional objective functions.Fuzzy entropy effectively quantifies uncertainty,leading to more well-defined clusters,improved noise robustness,and better preservation of anatomical structures in MRI images.Despite these advantages,the iterative nature of GWO and FCM introduces significant computational overhead,which restricts their applicability to high-resolution medical images.To overcome this bottleneck,we propose a Parallelized-GWO-based FCM(P-GWO-FCM)approach using GPU acceleration,where both GWO optimization and FCM updates(centroid computation and membership matrix updates)are parallelized.By concurrently executing these processes,our approach efficiently distributes the computational workload,significantly reducing execution time while maintaining high segmentation accuracy.The proposed parallel method,P-GWO-FCM,was evaluated on both simulated and clinical brain MR images,focusing on segmenting white matter,gray matter,and cerebrospinal fluid regions.The results indicate significant improvements in segmentation accuracy,achieving a Jaccard Similarity(JS)of 0.92,a Partition Coefficient Index(PCI)of 0.91,a Partition Entropy Index(PEI)of 0.25,and a Davies-Bouldin Index(DBI)of 0.30.Experimental comparisons demonstrate that P-GWO-FCM outperforms existing methods in both segmentation accuracy and computational efficiency,making it a promising solution for real-time medical image segmentation.
基金funded by the National Natural Science Foundation of China,grant number 62262045the Fundamental Research Funds for the Central Universities,grant number 2023CDJYGRH-YB11the Open Funding of SUGON Industrial Control and Security Center,grant number CUIT-SICSC-2025-03.
文摘Autonomous vehicles rely heavily on accurate and efficient scene segmentation for safe navigation and efficient operations.Traditional Bird’s Eye View(BEV)methods on semantic scene segmentation,which leverage multimodal sensor fusion,often struggle with noisy data and demand high-performance GPUs,leading to sensor misalignment and performance degradation.This paper introduces an Enhanced Channel Attention BEV(ECABEV),a novel approach designed to address the challenges under insufficient GPU memory conditions.ECABEV integrates camera and radar data through a de-noise enhanced channel attention mechanism,which utilizes global average and max pooling to effectively filter out noise while preserving discriminative features.Furthermore,an improved fusion approach is proposed to efficiently merge categorical data across modalities.To reduce computational overhead,a bilinear interpolation layer normalizationmethod is devised to ensure spatial feature fidelity.Moreover,a scalable crossentropy loss function is further designed to handle the imbalanced classes with less computational efficiency sacrifice.Extensive experiments on the nuScenes dataset demonstrate that ECABEV achieves state-of-the-art performance with an IoU of 39.961,using a lightweight ViT-B/14 backbone and lower resolution(224×224).Our approach highlights its cost-effectiveness and practical applicability,even on low-end devices.The code is publicly available at:https://github.com/YYF-CQU/ECABEV.git.
文摘This study aimed to enhance the performance of semantic segmentation for autonomous driving by improving the 2DPASS model.Two novel improvements were proposed and implemented in this paper:dynamically adjusting the loss function ratio and integrating an attention mechanism(CBAM).First,the loss function weights were adjusted dynamically.The grid search method is used for deciding the best ratio of 7:3.It gives greater emphasis to the cross-entropy loss,which resulted in better segmentation performance.Second,CBAM was applied at different layers of the 2Dencoder.Heatmap analysis revealed that introducing it after the second block of 2D image encoding produced the most effective enhancement of important feature representation.The training epoch was chosen for optimizing the best value by experiments,which improved model convergence and overall accuracy.To evaluate the proposed approach,experiments were conducted based on the SemanticKITTI database.The results showed that the improved model achieved higher segmentation accuracy by 64.31%,improved 11.47% in mIoU compared with the conventional 2DPASS model(baseline:52.84%).It was more effective at detecting small and distant objects and clearly identifying boundaries between different classes.Issues such as noise and variations in data distribution affected its accuracy,indicating the need for further refinement.Overall,the proposed improvements to the 2DPASS model demonstrated the potential to advance semantic segmentation technology and contributed to a more reliable perception of complex,dynamic environments in autonomous vehicles.Accurate segmentation enhances the vehicle’s ability to distinguish different objects,and this improvement directly supports safer navigation,robust decision-making,and efficient path planning,making it highly applicable to real-world deployment of autonomous systems in urban and highway settings.
基金supported under the 2024 Foshan City Self-Funded Science and Technology Innovation Project“Research on Image Segmentation Technology Based on Convolutional Neural Networks in Crop Images”(Project Number:2420001004686).
文摘The rising need for precision farming and sustainable land management has catalyzed the requirement for sophisticated means of deriving practical data from remote sensing images.Image segmentation,or the process of dividing the image into semantically relevant parts,has become a groundbreaking technology that allows resolving the problem of transitioning the pixel-level data to a parcel-level analysis.This review is a synthesis of the segmentation methods and their use in crop research and geospatial science.The architectures of pixel-based,object-based,and deep learning(convolutional neural networks,U-Net,Mask R-CNN,and Transformer models)are considered in terms of principles,capabilities,and limitations.Multi-spectral,hyperspectral,LiDAR,and SAR data are integrated to improve the efficiency of segmentation,allowing the possible delineation of fields,the classification of crops,health monitoring,monitoring of yields,and stress identification.In addition to agriculture,segmentation helps in land use and land cover mapping,identification of temporal change,monitoring of the environment,and is used in combination with GIS-based spatial modeling.Nevertheless,issues related to data heterogeneity,mixed pixels,computational requirements,and inadequate availability of labelled data still exist despite the major progress.The future directions involve multi-source data fusion,pixel-to-parcel pipeline automation,and predictive models based on AI,which are used to enhance its scalability,robustness,and the ability to monitor in real-time.This review makes it clear that the use of image segmentation as a tool in generating precision agriculture,sustainable land use,and informed geospatial.
