Background： The health of chickens and the welfare of poultry industry are central to the efforts of addressing global food security. Therefore, it is essential to study chicken immunology to maintain and improve its...Background： The health of chickens and the welfare of poultry industry are central to the efforts of addressing global food security. Therefore, it is essential to study chicken immunology to maintain and improve its health and to find novel and sustainable solutions. This paper presents a study on investigation of the effect of Scutellaria baicalensis root（SBR） on the immune response of broiler chicken, especially on lymphocytes and heterophils reactivity, regarding their contribution to the development of immunity of the chickens.Methods： The 121-day-old Hubbard Hi-Y male broiler hybrids were randomly assigned to four treatment groups,three SBR supplemented groups（0.5, 1.0, and 1.5% of SBR） and one control group. Each treatment was replicated five times with six birds per replicate pen in a battery brooder. Blood was collected after 3-（rd） and 6-（th）wk of the experiment, and hemoglobin and hematocrit values were determined, as well as total leukocyte count and differential count were performed. Nitroblue tetrazolium test and phagocytosis assay as nonspecific immune parameters and humoral immune responses to the antigenic challenge by sheep red blood cells were performed.Moreover, the ability of peripheral blood lymphocytes to form radial segmentation（RS） of their nuclei was analyzed.Body weight and relative weight of spleen, liver, and bursa of Fabricius were recorded.Results： Results showed that mean heterophile/lymphocyte ratio increased in the SBR groups compared to the control group and the blood of the chickens showed lymphocytic depletion. The results also demonstrated that the relative weight of bursa of Fabricius and spleen in groups fed with SBR significantly decreased compared to the control group. This study also showed that the addition of SBR significantly inhibited the formation of RS of nuclei compared to some cytotoxic substances.Conclusion： We found that SBR supplementation should be carefully evaluated when given to poultry. The excess intake of SBR supplementation may cause immunologic inhibition and may negatively affect the development of immune organs. SBR has inhibited the formation of radial segmentation nuclei showing antimetastatic properties and also the phagocytosis of chicken heterophils.展开更多
The 5.12 Wenchuan Earthquake in 2008 induced hundreds of large-scale landslides. This paper systematically analyzes 112 large-scale landslides (surface area > 50000 m2), which were identified by interpretation of r...The 5.12 Wenchuan Earthquake in 2008 induced hundreds of large-scale landslides. This paper systematically analyzes 112 large-scale landslides (surface area > 50000 m2), which were identified by interpretation of remote sensing imagery and field investigations. The analysis suggests that the distribution of large-scale landslides is affected by the following four factors: (a) distance effect: 80% of studied large-scale landslides are located within a distance of 5 km from the seismic faults. The farther the distance to the faults, the lower the number of large-scale landslides; (b) locked segment effect: the large-scale landslides are mainly located in five concentration zones closely related with the crossing, staggering and transfer sections between one seismic fault section and the next one, as well as the end of the NE fault section. The zone with the highest concentration was the Hongbai-Chaping segment, where a great number of large-scale landslides including the two largest landslides were located. The second highest concentration of large-scale landslides was observed in the Nanba-Donghekou segment at the end of NE fault, where the Donghekou landslide and the Woqian landslide occurred; (c) Hanging wall effect: about 70% of the large-scale landslides occurred on the hanging wall of the seismic faults; and (d) direction effect: in valleys perpendicular to the seismic faults, the density of large-scale landslides on the slopes facing the seismic wave is obviously higher than that on the slopes dipping in the same direction as the direction of propagation of the seismic wave. Meanwhile, it is found that the sliding and moving directions of large-scale landslides are related to the staggering direction of the faults in each section. In Qingchuan County where the main fault activity was horizontal twisting and staggering, a considerable number of landslides showed the feature of sliding and moving in NE direction which coincides with the staggering direction of the seismic faults.展开更多
基金supported by the Wroclaw Center for Biotechnology program KNOW(National Scientific Leadership Center)for the 2014–2018 award to BK
文摘Background： The health of chickens and the welfare of poultry industry are central to the efforts of addressing global food security. Therefore, it is essential to study chicken immunology to maintain and improve its health and to find novel and sustainable solutions. This paper presents a study on investigation of the effect of Scutellaria baicalensis root（SBR） on the immune response of broiler chicken, especially on lymphocytes and heterophils reactivity, regarding their contribution to the development of immunity of the chickens.Methods： The 121-day-old Hubbard Hi-Y male broiler hybrids were randomly assigned to four treatment groups,three SBR supplemented groups（0.5, 1.0, and 1.5% of SBR） and one control group. Each treatment was replicated five times with six birds per replicate pen in a battery brooder. Blood was collected after 3-（rd） and 6-（th）wk of the experiment, and hemoglobin and hematocrit values were determined, as well as total leukocyte count and differential count were performed. Nitroblue tetrazolium test and phagocytosis assay as nonspecific immune parameters and humoral immune responses to the antigenic challenge by sheep red blood cells were performed.Moreover, the ability of peripheral blood lymphocytes to form radial segmentation（RS） of their nuclei was analyzed.Body weight and relative weight of spleen, liver, and bursa of Fabricius were recorded.Results： Results showed that mean heterophile/lymphocyte ratio increased in the SBR groups compared to the control group and the blood of the chickens showed lymphocytic depletion. The results also demonstrated that the relative weight of bursa of Fabricius and spleen in groups fed with SBR significantly decreased compared to the control group. This study also showed that the addition of SBR significantly inhibited the formation of RS of nuclei compared to some cytotoxic substances.Conclusion： We found that SBR supplementation should be carefully evaluated when given to poultry. The excess intake of SBR supplementation may cause immunologic inhibition and may negatively affect the development of immune organs. SBR has inhibited the formation of radial segmentation nuclei showing antimetastatic properties and also the phagocytosis of chicken heterophils.
基金sponsored by the project of the Chinese National Key Basic Research Program on "The failure mechanism and distribution rule of slopes under strong earthquakes" (Grant No. 2008CB425801)the Education Department Innovation Research Team Program (Grant No. IRT0812)
文摘The 5.12 Wenchuan Earthquake in 2008 induced hundreds of large-scale landslides. This paper systematically analyzes 112 large-scale landslides (surface area > 50000 m2), which were identified by interpretation of remote sensing imagery and field investigations. The analysis suggests that the distribution of large-scale landslides is affected by the following four factors: (a) distance effect: 80% of studied large-scale landslides are located within a distance of 5 km from the seismic faults. The farther the distance to the faults, the lower the number of large-scale landslides; (b) locked segment effect: the large-scale landslides are mainly located in five concentration zones closely related with the crossing, staggering and transfer sections between one seismic fault section and the next one, as well as the end of the NE fault section. The zone with the highest concentration was the Hongbai-Chaping segment, where a great number of large-scale landslides including the two largest landslides were located. The second highest concentration of large-scale landslides was observed in the Nanba-Donghekou segment at the end of NE fault, where the Donghekou landslide and the Woqian landslide occurred; (c) Hanging wall effect: about 70% of the large-scale landslides occurred on the hanging wall of the seismic faults; and (d) direction effect: in valleys perpendicular to the seismic faults, the density of large-scale landslides on the slopes facing the seismic wave is obviously higher than that on the slopes dipping in the same direction as the direction of propagation of the seismic wave. Meanwhile, it is found that the sliding and moving directions of large-scale landslides are related to the staggering direction of the faults in each section. In Qingchuan County where the main fault activity was horizontal twisting and staggering, a considerable number of landslides showed the feature of sliding and moving in NE direction which coincides with the staggering direction of the seismic faults.
