This study quantitatively analyzes the effects of cloud seeding on precipitation and seasonal variations over the Boryeong Dam region,which has the lowest dam storage in South Korea,based on a one-year numerical simul...This study quantitatively analyzes the effects of cloud seeding on precipitation and seasonal variations over the Boryeong Dam region,which has the lowest dam storage in South Korea,based on a one-year numerical simulation for2021.The Morrison microphysics scheme in the WRF(Weather Research and Forecasting)model was modified to estimate differences in precipitation between simulations with seeding materials(Ag I and Ca Cl2;SEED)and without them(UNSD).The effect of cloud seeding on increasing precipitation or artificial rainfall(AR)between the two simulations was highest in August(average:0.21 mm;31%of the SEED-simulated monthly mean)and lowest in January(average:0.003 mm;30%).This large AR may be attributable to a combination of abundant moisture from the summer monsoon climate and enhanced cloud droplet growth resulting from cloud seeding.In the analysis of seasonal representative cases,cloud seeding demonstrated more pronounced effects in spring and summer,with mean 180-min accumulated AR values of 0.46 and 0.43 mm,respectively,within the study area.In the spring,where an actual flight experiment was conducted,the simulated mean180-min accumulated AR(1.41 mm)in the flight experiment area was close to the observed value(1.61 mm)for the same area.Additionally,cloud seeding promoted the hygroscopic growth of water vapor,thereby reducing the cloud water mixing ratio and increasing the rain water mixing ratio.Seasonal cross-sectional analysis further highlighted the impact of cloud seeding on changes in these two mixing ratios,with the most pronounced effects observed in spring and summer.展开更多
In order to study the effect of rice straw returning and seeding patterns on seedling emergence and grain yield, field experiment was conducted to investi- gate the effects of straw mechanized returning and different ...In order to study the effect of rice straw returning and seeding patterns on seedling emergence and grain yield, field experiment was conducted to investi- gate the effects of straw mechanized returning and different seeding patterns on e- mergence rate, emergence uniformity and yield traits of wheat after rice. The results were as follows： in rice straw removal treatments, the emergence rate of mechani- cal seeding in drill was lower than that of mechanical uniform planting and manual broadcast sowing, which were 51.84%, 90.89% and 88.87%, respectively; the emer- gence uniformity of manual broadcast sowing was inferior to mechanical seeding in drill and mechanical uniform planting, which were 0.49, 0.26 and 0.23, respectively. As for the treatments with rice straw returning to the field, the emergence rate and emergence uniformity all decreased in the three seeding patterns, of which mechan- ical seeding in drill dropped markedly with emergence rate decreased by 36.54%. The emergence rate and emergence uniformity affected grain yield by affecting pan- icle, grains per spike and 1 000-grain weight. The grain yield for the treatment with rice straw removal was 6 091.34 kg/hm2, while that with rice straw returning to field was 6 476.20 kg/hm2, and both were higher than the yields of the other two seed- ing patterns. Therefore, mechanical uniform planting was？recommended for its higher emergence rate, better emergence uniformity, which was conductive to increase grain yield in wheat after rice production with rice straw returning to field.展开更多
ObjectiveThis study aimed to investigate the effect of fulvic acid on the growth and yield components of direct seeding rice （Nanjing 44）. MethodThe rice seeds were soaked in 0 （water as a control）, 1, 2, 4 and 6 ...ObjectiveThis study aimed to investigate the effect of fulvic acid on the growth and yield components of direct seeding rice （Nanjing 44）. MethodThe rice seeds were soaked in 0 （water as a control）, 1, 2, 4 and 6 g/L fulvic acid （FA） before sowed. Then, the rice morphological indices, leaf chlorophyll content, photosynthesis parameters, root activity and chlorophyll fluorescence parameters were measured in the following field studies. ResultCompared with the control, the leaf area index, chlorophyll content, net photosynthetic rate, stomatal conductance and dry matter weight and some fluorescence parameters such as the maximum photochemical efficiency of photosystem II （PSII） （Fv/Fm）, excitation energy capture efficiency of opened PSII reaction center （Fv’/Fm’） and efficiency of the open reaction centre （ΦPS II） increased by different levels at both jointing stage and heading stage of direct seeding rice, whose seeds were soaked by FA with different concentrations. High FA concentration （4-6 g/L） significantly increased the cultivated rice leaf area index, chlorophyll content, net photosynthetic rate, stomatal conductance, transpiration rate and dry matter weight by 10.32% -22.88% , 5.88% -13.11% , 12.16% -26.84% , 11.43% -88.46% , 10.63% -21.63% , 18.49% -19.68% , respectively, thereby improving the physiological function and light energy transform efficiency of rice at the growth stage. With FA concentration increasing, the yield, effective panicles, grain number per panicle and seed setting rate were increased significantly compared with the control by 17.52%-18.71%, 3.46%-3.85%, 6.30%- 6.51% and 7.82%-8.69% respectively. ConclusionSoaking rice seed with FA could be considered as an effective way to improve the rice competitiveness at early growth stage.展开更多
In the east of Hunan Province, the research introduced new-type special controI ed-reIease fertiIizer for rapeseed, ＂Yishizhuang＂, and expIored its appIications in direct-seeding of rapeseed production. The resuIts ...In the east of Hunan Province, the research introduced new-type special controI ed-reIease fertiIizer for rapeseed, ＂Yishizhuang＂, and expIored its appIications in direct-seeding of rapeseed production. The resuIts showed that the appIication of special controI ed reIease fertiIizer for rapeseeds avoids earIy decIine of rapeseed resuIting from fertiIizer Ioss by one-off appIication, improves the number of green Ieaf before winter, and extends photosynthetic Iifespan of pods, which significantIy increases yields. What＆#39; more, with higher output-input ratio, the fertiIizer takes ad-vantages in saving cost, Iabor and increasing yield.展开更多
Hybrid rice Xinhunyou No. 6 could be cultivated by mixing male and fe- male parents and performing seedling raising followed by transplanting, mechanical planting or mechanical direct seeding according to the producti...Hybrid rice Xinhunyou No. 6 could be cultivated by mixing male and fe- male parents and performing seedling raising followed by transplanting, mechanical planting or mechanical direct seeding according to the production methods of com- mercial rice. During flowering stage, leaf cutting, application of ＂920＂ and pollination were conducted; after pollination, bentazon with a certain concentration was sprayed to kill the male parent; and the hybrid rice was harvested mechanically. Before storage, color separation was performed to remove little remaining male parent, thereby achieving whole-process mechanization of hybrid rice seed production. This study introduced mechanized seed production of Xinhunyou No. 6 through mixed- seeding from the aspects including mechanical direct seeding, seeding raising fol- lowed by transplanting and mechanical planting.展开更多
[Objective] The experiment was conducted to study suitable date of seed- ing and density of spring potato at the stock breeding base in Ebian County at an elevation of 1 200 to 1 500 m. [Methods] Virus-free Potato ＂C...[Objective] The experiment was conducted to study suitable date of seed- ing and density of spring potato at the stock breeding base in Ebian County at an elevation of 1 200 to 1 500 m. [Methods] Virus-free Potato ＂Chuanyu 13＂ was used as material to study the effects of date of seeding and density on growing period, germination rate, yield and water use efficiency of spring potato in the field. [Result] With the postponement of date of seeding, the days from sowing to germination shortened, while the germination rate, the number of tubers per plant, the number of middle and small tubers in a group, yield and water use efficiency all increased. Planting density had no effects on the days from sowing to germination and the ger- mination rate, while the number of tubers per ptant, the number of middle and small tubers in a group, yield and water use efficiency increased significantly along with the increasing planting density. [Conclusion] At an elevation of 1 200 m to 1 250 m in Ebian County, the suitable date of seeding for potato was from February 9 to March 1, and the suitable planting density was 12×10^4 plants per hm^2, however, in the optimum planting density has not been found so that it needs further research,展开更多
探讨基于“三全育人”教育理念的护理本科生SEED数字化学风建设,即以学风(style of study)建设为核心,教育(education)为驱动,在线学习(e-learning)为衔接,数字化(digitization)手段为支撑。通过数字化技术整合教育资源,优化学生的学习...探讨基于“三全育人”教育理念的护理本科生SEED数字化学风建设,即以学风(style of study)建设为核心,教育(education)为驱动,在线学习(e-learning)为衔接,数字化(digitization)手段为支撑。通过数字化技术整合教育资源,优化学生的学习行为,推动全员参与、全过程管理和全方位覆盖的学风建设模式,以提升护理本科生的学习投入度和信息素养为主要目标,为护理教育提供新的实践路径。展开更多
The shift from seedling transplanting to direct-seeding cultivation in rice demands robust root systems for early seedling establishment and yield stability.While the pleiotropic gene OsSP3(also designated TAC4 or SG2...The shift from seedling transplanting to direct-seeding cultivation in rice demands robust root systems for early seedling establishment and yield stability.While the pleiotropic gene OsSP3(also designated TAC4 or SG2)is known to regulate aboveground traits,including tiller angle,grain size,and panicle development,its function in root morphogenesis remains uncharacterized.展开更多
The plant life cycle and the promise of crop yield start with successful seed germination,which requires an optimal balance between the phytohormones abscisic acid(ABA)and gibberellin(GA).Here,we report that the APETA...The plant life cycle and the promise of crop yield start with successful seed germination,which requires an optimal balance between the phytohormones abscisic acid(ABA)and gibberellin(GA).Here,we report that the APETALA 2-type transcription factor SALT AND ABA RESPONSE ERF 1(OsSAE1)antagonistically modulates ABA and GA signaling to control seed germination in rice(Oryza sativa L.).We show that knocking out OsSAE1 delays seed germination,concomitant with the accumulation of SLENDER RICE1(OsSLR1),a GA signaling repressor DELLA protein;importantly,GA application rescued the seed germination defect of ossae1 mutants.OsSAE1 directly activates transcription of the GA biosynthesis gene OsKS1 and represses that of the GA metabolism gene OsGA2ox3,resulting in higher GA levels.Moreover,OsSLR1physically interacts with ABA-INSENSITIVE 5(OsABI5),a key ABA signaling component,enhancing the transcriptional activation capacity of OsABI5 toward its target genes to regulate seed germination.The temporal expression pattern of OsSAE1 supports its role in orchestrating GA and ABA signaling to modulate seed germination and seed dormancy.Different OsSAE1 haplotypes differentially affected OsSAE1 transcript levels and seed germination rates,illustrating the potential of the elite OsSAE1 haplotype for genetic improvement of seed germination.Overall,our study reveals that OsSAE1 controls rice seed germination by regulating the balance between ABA and GA,providing a pivotal selection target for breeding rice cultivars suitable for direct seeding.展开更多
Gibberellins(GAs)and auxin play central regulatory roles in seed germination and root system development,respectively,so that the application of these phytohormones to crops would be worthwhile,with an increasing pote...Gibberellins(GAs)and auxin play central regulatory roles in seed germination and root system development,respectively,so that the application of these phytohormones to crops would be worthwhile,with an increasing potential demand in agriculture.However,there are few effective chemicals that simultaneously enhance both GA and auxin signaling.Here,we report on an artificial thiourea derivative chemical,Y21,that serves as both a GA-signaling agonist and an auxin analog,promoting seed germination and root development,as well as low-phosphorus tolerance.Phenotypic,biochemical,and genetic evidence demonstrated that Y21 enhances the interaction between GA and its receptor GID1C via the Val239 amino acid residue and consequently promotes degradation of the DELLA proteins REPRESSOR OF ga1-3(RGA)and RGA-LIKE 2.Furthermore,we found that Y21 interacts with the auxin receptor TIR1 via the Cys405 residue and thus promotes the turnover of the auxinresponsive Aux/IAA proteins.Consequently,Y21significantly increases low-phosphorus tolerance of treated plants by positively regulating lateral root development.To our knowledge,Y21 is the first GA-signaling agonist to be identified,and our results also demonstrate that this potent synthetic chemical,identified by chemical genetic screening,is effective at modulating plant development and stress tolerance.展开更多
[Objectives]To provide a reference for the efficient use of wild Paeonia suffruticosa germplasm resources and the scientific selection of parents.[Methods]This study systematically evaluated cross-compatibility by con...[Objectives]To provide a reference for the efficient use of wild Paeonia suffruticosa germplasm resources and the scientific selection of parents.[Methods]This study systematically evaluated cross-compatibility by conducting interspecific crosses among the major wild species subsect.Delavayanae(Paeonia delavayi,Paeonia lutea,Paeonia potaninii,and Paeonia ludlowii)as well as reciprocal crosses between these species and cultivated varieties from the subsect.Vaginatae.[Results]Crosses among P.delavayi,P.lutea,and P.potaninii generally exhibited good seed set,with the resulting seeds showing a high emergence rate.In contrast,crosses involving P.ludlowii as a parent produced only a limited number of seeds,none of which germinated.In crosses with cultivated varieties where the wild species served as the maternal parent,the average seed set ranked as follows:P.lutea>P.delavayi>P.ludlowii>P.potaninii.When wild species were used as paternal parent,the average seed set ranked as follows:P.potaninii>P.lutea>P.delavayi>P.ludlowii.On the whole,most crosses between wild species of subsect.Delavayanae and cultivated varieties achieved normal seed set,demonstrating a certain degree of cross-compatibility.However,when the wild species served as the maternal parent,both the seed set and seedling emergence rate were superior to those in the reciprocal crosses(with wild species as the paternal parent).[Conclusions]Significant differences in cross-compatibility were observed among species within Paeonia subsect.Delavayanae.In addition,a significant maternal effect was identified in crosses between wild species of this subsection and cultivated varieties.These findings suggest that by selecting highly compatible combinations,there remains considerable potential for utilizing these wild species in peony breeding.展开更多
Salinity is one of the major abiotic stresses limiting chickpea(Cicer arietinum L.)productivity,particularly in arid and semi-arid regions where soil salinization is intensifying.Developing cost-effective and practica...Salinity is one of the major abiotic stresses limiting chickpea(Cicer arietinum L.)productivity,particularly in arid and semi-arid regions where soil salinization is intensifying.Developing cost-effective and practical strategies to enhance seedling establishment and early vigor under saline conditions is therefore essential.In this study,we compared two seed-priming agents-1 mM proline and 25 mM NaCl-under identical hydroponic conditions to elucidate tissue-specific responses to 25 mM NaCl stress.Proline priming significantly improved shoot length(by~23%),total chlorophyll content(by~19%),and ascorbate peroxidase(ASPOX)activity.In contrast,NaCl priming enhanced root biomass retention(by~38%)and peroxidase(POD)activity under salinity stress.Both priming treatments induced higher proline accumulation and antioxidant capacity,though with tissue-specific effects:proline favored aboveground resilience,while NaCl strengthened root ionic and oxidative balance.These findings highlight the complementary nature of proline and NaCl priming and support the concept of stress“memory,”whereby plants acquire enhanced readiness to cope with salinity.Integrating such priming strategies into chickpea cultivation could contribute to improved yield stability and sustainability in saline agroecosystems.展开更多
Seaweed extract contains plant growth regulators and bio-stimulants that enhance plant growth and development.In Bangladesh,winter rice(Boro rice)in the nursery bed often shows poor seed emergence and weak seedling gr...Seaweed extract contains plant growth regulators and bio-stimulants that enhance plant growth and development.In Bangladesh,winter rice(Boro rice)in the nursery bed often shows poor seed emergence and weak seedling growth due to low temperature.This problem can be addressed by using seaweed extract as a seed priming agent and bio-stimulant.The objective of this study was to evaluate the effectiveness of seaweed extract(Crop Plus)on seed emergence,seedling growth,and vigor of winter rice in the nursery.Two experiments were conducted at Bangladesh Agricultural University using BRRI dhan89.The laboratory experiment consisted of 17 treatments combining three concentrations of Crop Plus(5000,10,000 and 15,000 ppm)and four priming durations(6,12,18,and 24 h),along with hydro-priming and a no priming as control.Seed priming with 15,000 ppm for 24 h produced the highest germination percentage and superior seedling growth traits.The nursery bed experiment comprised 11 treatments combining two doses(1 mL m^(−2)and 2 mL m^(−2))of Crop Plus and five different foliar application schedules,along with a control.All treatments outperformed the control,with the best results from Crop Plus@2 mL m^(−2)applied at 20 and 30 days after sowing(DAS).Overall,the treatment involving seed priming with 15,000 ppm seaweed extract for 24 h,followed by nursery application at 2 mL m^(−2)at 20 and 30 DAS,resulted in higher germination and improved early growth of winter rice.However,further validation across multiple locations,seasons,and rice cultivars is recommended.展开更多
Soybean is an important source of oil,protein,and feed.However,its yield is far below that of major cereal crops.The green revolution increased the yield of cereal crops partially through high-density planting of lodg...Soybean is an important source of oil,protein,and feed.However,its yield is far below that of major cereal crops.The green revolution increased the yield of cereal crops partially through high-density planting of lodging-resistant semi-dwarf varieties,but required more nitrogen fertilizers,posing an environmental threat.Genes that can improve nitrogen use efficiency need to be integrated into semi-dwarf varieties to avoid the overuse of fertilizers without the loss of dwarfism.Unlike cereal crops,soybean can assimilate atmospheric nitrogen through symbiotic bacteria.Here,we created new alleles of Gm GID1-2(Glycine max GIBBERELLIN INSENSITIVE DWARF 1-2)using clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated nuclease 9(Cas9)editing,which improved soybean architecture,yield,seed oil content,and nitrogen fixation,by regulation of important pathways and known genes related to branching,lipid metabolism,and nodule symbiosis.Gm GID1-2 knockout reduced plant height,and increased stem diameter and strength,number of branches,nodes on the primary stem,pods,and seeds per plant,leading to an increase in seed weight per plant and yield in soybean.The nodule number,nodule weight,nitrogenase activity,and nitrogen content were also improved in Gm GID1-2knockout soybean lines,which is novel compared with the semi-dwarf genes in cereal crops.No loss-of-function allele for Gm GID1-2 was identified in soybean germplasm and the edited Gm GID1-2s are superior to the natural alleles,suggesting the Gm GID1-2 knockout mutants generated in this study are valuable genetic resources to further improve soybean yield and seed oil content in future breeding programs.This study illustrates the pleiotropic functions of the GID1 knockout alleles with positive effects on plant architecture,yield,and nitrogen fixation in soybean,which provides a promising strategy toward sustainable agriculture.展开更多
Date palm(Phoenix dactylifera L.)seeds,often discarded as by-products during date fruit processing,represent approximately 6-20%of the fruit’s mass depending on cultivar and maturity stage.Although traditionally used...Date palm(Phoenix dactylifera L.)seeds,often discarded as by-products during date fruit processing,represent approximately 6-20%of the fruit’s mass depending on cultivar and maturity stage.Although traditionally used for producing activated charcoal and in environmental remediation,their pharmacological potential remains underexplored.This study aimed to evaluate the chemical composition,biological activity,and potential commercial value of seeds from four widely consumed Saharan cultivars(Assiane,Boufegouss,Aziza,and Majhoul)collected in the Figuig region.Soxhlet extraction was employed to obtain seed extracts,which were subsequently characterized by qualitative and quantitative phytochemical screening.The analyses revealed the presence of polyphenols,flavonoids,terpenoids,steroids,alkaloids,and other secondary metabolites.The Aziza and Boufegouss varieties were particularly rich in flavonoids(82.09-139.61 mg quercetin equivalents/100 g dry matter)and total polyphenols(69.81-1183.96 mg gallic acid equivalents/100 g dry matter).Mineral profiling confirmed the occurrence of essential trace elements,including Zn,Cu,Fe,and Co.To investigate their antidiabetic potential,in vitro assays ofα-glucosidase andα-amylase inhibition were performed.Acetone extracts demonstrated significant inhibitory activity against both enzymes,suggesting a promising role of P.dactylifera seed extracts as natural agents for the management of type 2 diabetes mellitus.展开更多
Prevention of biological invasion requires understanding how alien species invade native communities.Although studies have identified mechanisms that underlie plant invasion in some habitats,limited attention has focu...Prevention of biological invasion requires understanding how alien species invade native communities.Although studies have identified mechanisms that underlie plant invasion in some habitats,limited attention has focused on invasion patterns along elevational gradients.In this study,we asked which factors drive the global and regional distribution of the invasive plant Galinsoga quadriradiata along elevational gradients.To answer this question,we examined whether human activities(i.e.,roads)promote G.quadriradiata invasion,how seed dispersal-related traits of G.quadriradiata change along elevation gradients,and whether G.quadriradiata has adapted to high-elevation environments through phenotypic plasticity or genetic variation.On the global scale,we found that human activities and road density positively contribute to the G.quadriradiata expansion in mountainous areas.Field surveys in China revealed significant elevational differences in the seed dispersal traits of G.quadriradiata,with higher-elevation populations exhibiting lower dispersal ability and generally lower genetic diversity.Under common conditions,high-elevation populations showed higher leaf mass ratio but lower root mass ratio and reproductive allocation.This suggests that high-elevation environments create a barrier to dispersal for G.quadriradiata,and that G.quadriradiata has adapted phenotypically to these conditions.Our study indicates that the elevational invasion pattern of G.quadriradiata is shaped by multiple factors,particularly human activities and phenotypic adaptability.In addition,our finding that G.quadriradiata invasion at high elevations is not constrained by low genetic diversity indicates that monitoring and management of G.quadriradiata in mountainous areas should be strengthened.展开更多
High temperature stress (HT) significantly reduces maize yield by impairing starch accumulation in kernels.However,the mechanism by which HT affects starch synthesis remains controversial-whether through reduced assim...High temperature stress (HT) significantly reduces maize yield by impairing starch accumulation in kernels.However,the mechanism by which HT affects starch synthesis remains controversial-whether through reduced assimilate supply or direct inhibition on kernel metabolism.To clarify these mechanisms,a heat-sensitive maize hybrid,Xianyu 335 (XY),was exposed to 30℃/20℃ (maximum/minimum temperature,control) and 40℃/30℃ for seven consecutive days during the seed setting stage.Synchronous pollination (SP),apical pollination (AP),and shading treatments were applied to manipulate the inherent source–sink ratio in maize plants.Results showed that apical kernel weight decreased by 11.9%under 40℃ in the SP treatment.The ^(13)C content,starch accumulation,and cell-wall invertase (CWIN) activity also declined by 15.9,36.7,and 16.4%,respectively,under HT.In the shading treatment,40℃/30℃ caused even greater reductions in^(13)C content,starch accumulation,and CWIN activity due to diminished assimilate supply.Conversely,in the AP treatment,starch content and CWIN activity increased by 22.0 and 18.5%,respectively,under 40℃/30℃,resulting in kernel weight and ^(13)C content similar to those in SP and shading treatments regardless of temperature.Consistent with apical kernels under AP,HT did not negatively affect middle kernels in either SP or shading treatments,as kernel weight and starch content remained unchanged under HT.Although all kernels were exposed to the same HT or control environment,their responses varied a lot.The impaired starch synthesis in apical kernels under HT was rescued by increasing carbon supply via AP treatment.The contrasting performance among middle kernels,apical kernels under AP,and apical kernels under SP or shading indicates that reduced carbon supply is a critical factor underlying inhibited starch accumulation.Our findings provide a theoretical basis for further understanding kernel abortion under HT.展开更多
In the seeding operations in order to mitigate the climatic changes or to intervene beneficently on the precipitations process, it is very important to know the roll of the critical radius size of the cloud drops form...In the seeding operations in order to mitigate the climatic changes or to intervene beneficently on the precipitations process, it is very important to know the roll of the critical radius size of the cloud drops formation and its posterior evolution. In the seeding operations programs, the fundament is to determinate the critical radius in order to obtain efficient results. So, it must consider （a） the critical radius size necessary in order to get the better results; （b） the atmospheric conditions that determine it. In order to get a methodology to calculate the critical radius in each atmospheric condition, the present work has been developed. And with them, it can estimate the nuclei size necessary in order to assure good seeding. The authors had obtained approximate values that were good enough to the goals.展开更多
Objective:To evaluate the nursing effects of auricular seed therapy combined with Chinese herbal poultice treatment for patients with lumbar disc herniation(LDH).Method:A total of 80 patients with LDH were randomly di...Objective:To evaluate the nursing effects of auricular seed therapy combined with Chinese herbal poultice treatment for patients with lumbar disc herniation(LDH).Method:A total of 80 patients with LDH were randomly divided into two groups.The combined group received auricular acupuncture with seed application combined with Chinese herbal poultice therapy,while the reference group received auricular acupuncture with seed application alone.The therapeutic outcomes of both groups were compared.Results:The combined intervention group demonstrated decreased sleep quality scores,reduced pain neurotransmitter levels,and improved lumbar spine function scores post-intervention,with significant intergroup differences(p<0.05).Conclusion:Auricular seed therapy combined with Chinese herbal poultice care effectively improves sleep quality and lumbar spine function while alleviating pain perception in patients with lumbar disc herniation,indicating superior therapeutic efficacy.展开更多
Emerging and powerful genome editing tools,particularly CRISPR/Cas9,are facilitating functional genomics research and accelerating crop improvement(Jiang et al.2021;Cao et al.2023;Chen C et al.2023;Liu et al.2023a).Ho...Emerging and powerful genome editing tools,particularly CRISPR/Cas9,are facilitating functional genomics research and accelerating crop improvement(Jiang et al.2021;Cao et al.2023;Chen C et al.2023;Liu et al.2023a).However,the detection and screening of transgenic lines remain major bottlenecks,being time-consuming,labor-intensive,and inefficient during transformation and subsequent mutation identification.A simple and efficient visual marker system plays a critical role in addressing these challenges.Recent studies demonstrated that the GmW1 and RUBY reporter systems were used to obtain visual transgenic soybean(Glycine max) plants(Chen L et al.2023;Chen et al.2024).展开更多
基金funded by the Korea Meteorological Administration Research and Development Program“Research on Weather Modification and Cloud Physics”(Grant No.KMA2018-00224)supported by Korea Institute of Marine Science&Technology Promotion(KIMST)funded by the Ministry of Oceans and Fisheries,Korea(RS-202502217872)supported by an NRF grant funded by the Korean government(MSIT)(Grant No.NRF2023R1A2C1002367)。
文摘This study quantitatively analyzes the effects of cloud seeding on precipitation and seasonal variations over the Boryeong Dam region,which has the lowest dam storage in South Korea,based on a one-year numerical simulation for2021.The Morrison microphysics scheme in the WRF(Weather Research and Forecasting)model was modified to estimate differences in precipitation between simulations with seeding materials(Ag I and Ca Cl2;SEED)and without them(UNSD).The effect of cloud seeding on increasing precipitation or artificial rainfall(AR)between the two simulations was highest in August(average:0.21 mm;31%of the SEED-simulated monthly mean)and lowest in January(average:0.003 mm;30%).This large AR may be attributable to a combination of abundant moisture from the summer monsoon climate and enhanced cloud droplet growth resulting from cloud seeding.In the analysis of seasonal representative cases,cloud seeding demonstrated more pronounced effects in spring and summer,with mean 180-min accumulated AR values of 0.46 and 0.43 mm,respectively,within the study area.In the spring,where an actual flight experiment was conducted,the simulated mean180-min accumulated AR(1.41 mm)in the flight experiment area was close to the observed value(1.61 mm)for the same area.Additionally,cloud seeding promoted the hygroscopic growth of water vapor,thereby reducing the cloud water mixing ratio and increasing the rain water mixing ratio.Seasonal cross-sectional analysis further highlighted the impact of cloud seeding on changes in these two mixing ratios,with the most pronounced effects observed in spring and summer.
文摘In order to study the effect of rice straw returning and seeding patterns on seedling emergence and grain yield, field experiment was conducted to investi- gate the effects of straw mechanized returning and different seeding patterns on e- mergence rate, emergence uniformity and yield traits of wheat after rice. The results were as follows： in rice straw removal treatments, the emergence rate of mechani- cal seeding in drill was lower than that of mechanical uniform planting and manual broadcast sowing, which were 51.84%, 90.89% and 88.87%, respectively; the emer- gence uniformity of manual broadcast sowing was inferior to mechanical seeding in drill and mechanical uniform planting, which were 0.49, 0.26 and 0.23, respectively. As for the treatments with rice straw returning to the field, the emergence rate and emergence uniformity all decreased in the three seeding patterns, of which mechan- ical seeding in drill dropped markedly with emergence rate decreased by 36.54%. The emergence rate and emergence uniformity affected grain yield by affecting pan- icle, grains per spike and 1 000-grain weight. The grain yield for the treatment with rice straw removal was 6 091.34 kg/hm2, while that with rice straw returning to field was 6 476.20 kg/hm2, and both were higher than the yields of the other two seed- ing patterns. Therefore, mechanical uniform planting was？recommended for its higher emergence rate, better emergence uniformity, which was conductive to increase grain yield in wheat after rice production with rice straw returning to field.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(201303022)National Key Technology Research and Development Program during the 12th Five-Year Plan Period(2012BAD19B02)~~
文摘ObjectiveThis study aimed to investigate the effect of fulvic acid on the growth and yield components of direct seeding rice （Nanjing 44）. MethodThe rice seeds were soaked in 0 （water as a control）, 1, 2, 4 and 6 g/L fulvic acid （FA） before sowed. Then, the rice morphological indices, leaf chlorophyll content, photosynthesis parameters, root activity and chlorophyll fluorescence parameters were measured in the following field studies. ResultCompared with the control, the leaf area index, chlorophyll content, net photosynthetic rate, stomatal conductance and dry matter weight and some fluorescence parameters such as the maximum photochemical efficiency of photosystem II （PSII） （Fv/Fm）, excitation energy capture efficiency of opened PSII reaction center （Fv’/Fm’） and efficiency of the open reaction centre （ΦPS II） increased by different levels at both jointing stage and heading stage of direct seeding rice, whose seeds were soaked by FA with different concentrations. High FA concentration （4-6 g/L） significantly increased the cultivated rice leaf area index, chlorophyll content, net photosynthetic rate, stomatal conductance, transpiration rate and dry matter weight by 10.32% -22.88% , 5.88% -13.11% , 12.16% -26.84% , 11.43% -88.46% , 10.63% -21.63% , 18.49% -19.68% , respectively, thereby improving the physiological function and light energy transform efficiency of rice at the growth stage. With FA concentration increasing, the yield, effective panicles, grain number per panicle and seed setting rate were increased significantly compared with the control by 17.52%-18.71%, 3.46%-3.85%, 6.30%- 6.51% and 7.82%-8.69% respectively. ConclusionSoaking rice seed with FA could be considered as an effective way to improve the rice competitiveness at early growth stage.
基金Supported by Modern Agricultural Industry Technology System(CARS-13)National Agricultural Science Technology Achievement Transformation Fund(2014GB2D200211)~~
文摘In the east of Hunan Province, the research introduced new-type special controI ed-reIease fertiIizer for rapeseed, ＂Yishizhuang＂, and expIored its appIications in direct-seeding of rapeseed production. The resuIts showed that the appIication of special controI ed reIease fertiIizer for rapeseeds avoids earIy decIine of rapeseed resuIting from fertiIizer Ioss by one-off appIication, improves the number of green Ieaf before winter, and extends photosynthetic Iifespan of pods, which significantIy increases yields. What＆#39; more, with higher output-input ratio, the fertiIizer takes ad-vantages in saving cost, Iabor and increasing yield.
基金Supported by Science and Technology Innovation Team of Anhui Academy of Agricultural Sciences(15C0108)Subject Construction Project of Anhui Academy of Agricultural Sciences(14A0102)Science and Technology Support Program of Ministry of Science and Technology(2012BAD07B01-3)~~
文摘Hybrid rice Xinhunyou No. 6 could be cultivated by mixing male and fe- male parents and performing seedling raising followed by transplanting, mechanical planting or mechanical direct seeding according to the production methods of com- mercial rice. During flowering stage, leaf cutting, application of ＂920＂ and pollination were conducted; after pollination, bentazon with a certain concentration was sprayed to kill the male parent; and the hybrid rice was harvested mechanically. Before storage, color separation was performed to remove little remaining male parent, thereby achieving whole-process mechanization of hybrid rice seed production. This study introduced mechanized seed production of Xinhunyou No. 6 through mixed- seeding from the aspects including mechanical direct seeding, seeding raising fol- lowed by transplanting and mechanical planting.
基金Supported by Project of Propagation of Improved Potatoes,Project of CropsLivestock and Poultry Breeding in 12th Five-Year Plan of Sichuan ProvinceTeam Project of Sichuan Potato Innovation under National Modern Industrial and Technological System~~
文摘[Objective] The experiment was conducted to study suitable date of seed- ing and density of spring potato at the stock breeding base in Ebian County at an elevation of 1 200 to 1 500 m. [Methods] Virus-free Potato ＂Chuanyu 13＂ was used as material to study the effects of date of seeding and density on growing period, germination rate, yield and water use efficiency of spring potato in the field. [Result] With the postponement of date of seeding, the days from sowing to germination shortened, while the germination rate, the number of tubers per plant, the number of middle and small tubers in a group, yield and water use efficiency all increased. Planting density had no effects on the days from sowing to germination and the ger- mination rate, while the number of tubers per ptant, the number of middle and small tubers in a group, yield and water use efficiency increased significantly along with the increasing planting density. [Conclusion] At an elevation of 1 200 m to 1 250 m in Ebian County, the suitable date of seeding for potato was from February 9 to March 1, and the suitable planting density was 12×10^4 plants per hm^2, however, in the optimum planting density has not been found so that it needs further research,
文摘探讨基于“三全育人”教育理念的护理本科生SEED数字化学风建设,即以学风(style of study)建设为核心,教育(education)为驱动,在线学习(e-learning)为衔接,数字化(digitization)手段为支撑。通过数字化技术整合教育资源,优化学生的学习行为,推动全员参与、全过程管理和全方位覆盖的学风建设模式,以提升护理本科生的学习投入度和信息素养为主要目标,为护理教育提供新的实践路径。
基金funded by the Major Science and Technology Projects of Zhejiang Province,China(Grant No.2021C02063-5)the Key Research and Development Projects of Hainan Province,China(Grant No.ZDYF2023XDNY086)+2 种基金the State Key Laboratory for Quality and Safety Hazard Factors and Risk Prevention and Control of Agricultural Products Jointly Constructed by the Ministry and the Province,China(Grant No.2010DS700124)the Zhejiang Province Vanguard Leading Goose Project,China(Grant Nos.2023C02055 and 2022C02034)the Jiaxing Nanhu District Science and Technology Plan Project,China(Grant No.2023017).
文摘The shift from seedling transplanting to direct-seeding cultivation in rice demands robust root systems for early seedling establishment and yield stability.While the pleiotropic gene OsSP3(also designated TAC4 or SG2)is known to regulate aboveground traits,including tiller angle,grain size,and panicle development,its function in root morphogenesis remains uncharacterized.
基金funded by the National Natural Science Foundation of China(32472037,32030079)the National Key Research and Development Program of China(2022YFD1201700)+2 种基金the Youth innovation of Chinese Academy of Agricultural Sciences(Y2024QC14)the Central Public-Interest Scientific Institution Basal Research Fund(1610392023004)Agricultural Science and Technology Innovation Program(CAAS-ZDRW202407)。
文摘The plant life cycle and the promise of crop yield start with successful seed germination,which requires an optimal balance between the phytohormones abscisic acid(ABA)and gibberellin(GA).Here,we report that the APETALA 2-type transcription factor SALT AND ABA RESPONSE ERF 1(OsSAE1)antagonistically modulates ABA and GA signaling to control seed germination in rice(Oryza sativa L.).We show that knocking out OsSAE1 delays seed germination,concomitant with the accumulation of SLENDER RICE1(OsSLR1),a GA signaling repressor DELLA protein;importantly,GA application rescued the seed germination defect of ossae1 mutants.OsSAE1 directly activates transcription of the GA biosynthesis gene OsKS1 and represses that of the GA metabolism gene OsGA2ox3,resulting in higher GA levels.Moreover,OsSLR1physically interacts with ABA-INSENSITIVE 5(OsABI5),a key ABA signaling component,enhancing the transcriptional activation capacity of OsABI5 toward its target genes to regulate seed germination.The temporal expression pattern of OsSAE1 supports its role in orchestrating GA and ABA signaling to modulate seed germination and seed dormancy.Different OsSAE1 haplotypes differentially affected OsSAE1 transcript levels and seed germination rates,illustrating the potential of the elite OsSAE1 haplotype for genetic improvement of seed germination.Overall,our study reveals that OsSAE1 controls rice seed germination by regulating the balance between ABA and GA,providing a pivotal selection target for breeding rice cultivars suitable for direct seeding.
基金supported by grants from the National Natural Science Foundation of China(32470364,31872850,and 31872804)the Natural Science Basic Research Program of Shaanxi(2025JC-JCQN-056 and 2024JC-YBMS-151)+2 种基金the Guangdong Basic and Applied Basic Research Foundation(2025A1515012749)the China Postdoctoral Science Foundation(2025M774348)the Shaanxi Fundamental Science Research Project for Chemistry&Biology(22JHZ007and 22JHQ054)。
文摘Gibberellins(GAs)and auxin play central regulatory roles in seed germination and root system development,respectively,so that the application of these phytohormones to crops would be worthwhile,with an increasing potential demand in agriculture.However,there are few effective chemicals that simultaneously enhance both GA and auxin signaling.Here,we report on an artificial thiourea derivative chemical,Y21,that serves as both a GA-signaling agonist and an auxin analog,promoting seed germination and root development,as well as low-phosphorus tolerance.Phenotypic,biochemical,and genetic evidence demonstrated that Y21 enhances the interaction between GA and its receptor GID1C via the Val239 amino acid residue and consequently promotes degradation of the DELLA proteins REPRESSOR OF ga1-3(RGA)and RGA-LIKE 2.Furthermore,we found that Y21 interacts with the auxin receptor TIR1 via the Cys405 residue and thus promotes the turnover of the auxinresponsive Aux/IAA proteins.Consequently,Y21significantly increases low-phosphorus tolerance of treated plants by positively regulating lateral root development.To our knowledge,Y21 is the first GA-signaling agonist to be identified,and our results also demonstrate that this potent synthetic chemical,identified by chemical genetic screening,is effective at modulating plant development and stress tolerance.
文摘[Objectives]To provide a reference for the efficient use of wild Paeonia suffruticosa germplasm resources and the scientific selection of parents.[Methods]This study systematically evaluated cross-compatibility by conducting interspecific crosses among the major wild species subsect.Delavayanae(Paeonia delavayi,Paeonia lutea,Paeonia potaninii,and Paeonia ludlowii)as well as reciprocal crosses between these species and cultivated varieties from the subsect.Vaginatae.[Results]Crosses among P.delavayi,P.lutea,and P.potaninii generally exhibited good seed set,with the resulting seeds showing a high emergence rate.In contrast,crosses involving P.ludlowii as a parent produced only a limited number of seeds,none of which germinated.In crosses with cultivated varieties where the wild species served as the maternal parent,the average seed set ranked as follows:P.lutea>P.delavayi>P.ludlowii>P.potaninii.When wild species were used as paternal parent,the average seed set ranked as follows:P.potaninii>P.lutea>P.delavayi>P.ludlowii.On the whole,most crosses between wild species of subsect.Delavayanae and cultivated varieties achieved normal seed set,demonstrating a certain degree of cross-compatibility.However,when the wild species served as the maternal parent,both the seed set and seedling emergence rate were superior to those in the reciprocal crosses(with wild species as the paternal parent).[Conclusions]Significant differences in cross-compatibility were observed among species within Paeonia subsect.Delavayanae.In addition,a significant maternal effect was identified in crosses between wild species of this subsection and cultivated varieties.These findings suggest that by selecting highly compatible combinations,there remains considerable potential for utilizing these wild species in peony breeding.
文摘Salinity is one of the major abiotic stresses limiting chickpea(Cicer arietinum L.)productivity,particularly in arid and semi-arid regions where soil salinization is intensifying.Developing cost-effective and practical strategies to enhance seedling establishment and early vigor under saline conditions is therefore essential.In this study,we compared two seed-priming agents-1 mM proline and 25 mM NaCl-under identical hydroponic conditions to elucidate tissue-specific responses to 25 mM NaCl stress.Proline priming significantly improved shoot length(by~23%),total chlorophyll content(by~19%),and ascorbate peroxidase(ASPOX)activity.In contrast,NaCl priming enhanced root biomass retention(by~38%)and peroxidase(POD)activity under salinity stress.Both priming treatments induced higher proline accumulation and antioxidant capacity,though with tissue-specific effects:proline favored aboveground resilience,while NaCl strengthened root ionic and oxidative balance.These findings highlight the complementary nature of proline and NaCl priming and support the concept of stress“memory,”whereby plants acquire enhanced readiness to cope with salinity.Integrating such priming strategies into chickpea cultivation could contribute to improved yield stability and sustainability in saline agroecosystems.
基金funded by Bangladesh Agricultural University Research System(BAURES)through the Project No.2024/48/BAU.
文摘Seaweed extract contains plant growth regulators and bio-stimulants that enhance plant growth and development.In Bangladesh,winter rice(Boro rice)in the nursery bed often shows poor seed emergence and weak seedling growth due to low temperature.This problem can be addressed by using seaweed extract as a seed priming agent and bio-stimulant.The objective of this study was to evaluate the effectiveness of seaweed extract(Crop Plus)on seed emergence,seedling growth,and vigor of winter rice in the nursery.Two experiments were conducted at Bangladesh Agricultural University using BRRI dhan89.The laboratory experiment consisted of 17 treatments combining three concentrations of Crop Plus(5000,10,000 and 15,000 ppm)and four priming durations(6,12,18,and 24 h),along with hydro-priming and a no priming as control.Seed priming with 15,000 ppm for 24 h produced the highest germination percentage and superior seedling growth traits.The nursery bed experiment comprised 11 treatments combining two doses(1 mL m^(−2)and 2 mL m^(−2))of Crop Plus and five different foliar application schedules,along with a control.All treatments outperformed the control,with the best results from Crop Plus@2 mL m^(−2)applied at 20 and 30 days after sowing(DAS).Overall,the treatment involving seed priming with 15,000 ppm seaweed extract for 24 h,followed by nursery application at 2 mL m^(−2)at 20 and 30 DAS,resulted in higher germination and improved early growth of winter rice.However,further validation across multiple locations,seasons,and rice cultivars is recommended.
基金supported by the National Natural Science Foundation of China(32372192)the Core Technology Development for Breeding Program of Jiangsu Province(JBGS-2021-014)Jiangsu Key Laboratory of Soybean Biotechnology and Intelligent Breeding(BM2024005)。
文摘Soybean is an important source of oil,protein,and feed.However,its yield is far below that of major cereal crops.The green revolution increased the yield of cereal crops partially through high-density planting of lodging-resistant semi-dwarf varieties,but required more nitrogen fertilizers,posing an environmental threat.Genes that can improve nitrogen use efficiency need to be integrated into semi-dwarf varieties to avoid the overuse of fertilizers without the loss of dwarfism.Unlike cereal crops,soybean can assimilate atmospheric nitrogen through symbiotic bacteria.Here,we created new alleles of Gm GID1-2(Glycine max GIBBERELLIN INSENSITIVE DWARF 1-2)using clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated nuclease 9(Cas9)editing,which improved soybean architecture,yield,seed oil content,and nitrogen fixation,by regulation of important pathways and known genes related to branching,lipid metabolism,and nodule symbiosis.Gm GID1-2 knockout reduced plant height,and increased stem diameter and strength,number of branches,nodes on the primary stem,pods,and seeds per plant,leading to an increase in seed weight per plant and yield in soybean.The nodule number,nodule weight,nitrogenase activity,and nitrogen content were also improved in Gm GID1-2knockout soybean lines,which is novel compared with the semi-dwarf genes in cereal crops.No loss-of-function allele for Gm GID1-2 was identified in soybean germplasm and the edited Gm GID1-2s are superior to the natural alleles,suggesting the Gm GID1-2 knockout mutants generated in this study are valuable genetic resources to further improve soybean yield and seed oil content in future breeding programs.This study illustrates the pleiotropic functions of the GID1 knockout alleles with positive effects on plant architecture,yield,and nitrogen fixation in soybean,which provides a promising strategy toward sustainable agriculture.
文摘Date palm(Phoenix dactylifera L.)seeds,often discarded as by-products during date fruit processing,represent approximately 6-20%of the fruit’s mass depending on cultivar and maturity stage.Although traditionally used for producing activated charcoal and in environmental remediation,their pharmacological potential remains underexplored.This study aimed to evaluate the chemical composition,biological activity,and potential commercial value of seeds from four widely consumed Saharan cultivars(Assiane,Boufegouss,Aziza,and Majhoul)collected in the Figuig region.Soxhlet extraction was employed to obtain seed extracts,which were subsequently characterized by qualitative and quantitative phytochemical screening.The analyses revealed the presence of polyphenols,flavonoids,terpenoids,steroids,alkaloids,and other secondary metabolites.The Aziza and Boufegouss varieties were particularly rich in flavonoids(82.09-139.61 mg quercetin equivalents/100 g dry matter)and total polyphenols(69.81-1183.96 mg gallic acid equivalents/100 g dry matter).Mineral profiling confirmed the occurrence of essential trace elements,including Zn,Cu,Fe,and Co.To investigate their antidiabetic potential,in vitro assays ofα-glucosidase andα-amylase inhibition were performed.Acetone extracts demonstrated significant inhibitory activity against both enzymes,suggesting a promising role of P.dactylifera seed extracts as natural agents for the management of type 2 diabetes mellitus.
基金supported by the National Natural Science Foundation of China(32271584 and 31600445)the Natural Science Basic Research Plan in Shaanxi Province of China(2020JM-286)+2 种基金the Fundamental Research Funds for the Central Universities(GK202103072,GK202103073)the National College Students'Innovative Entrepreneurial Training Plan Program(202310718085)Special Research Project in Philosophy and Social Sciences of Shaanxi Province(2022HZ1795).
文摘Prevention of biological invasion requires understanding how alien species invade native communities.Although studies have identified mechanisms that underlie plant invasion in some habitats,limited attention has focused on invasion patterns along elevational gradients.In this study,we asked which factors drive the global and regional distribution of the invasive plant Galinsoga quadriradiata along elevational gradients.To answer this question,we examined whether human activities(i.e.,roads)promote G.quadriradiata invasion,how seed dispersal-related traits of G.quadriradiata change along elevation gradients,and whether G.quadriradiata has adapted to high-elevation environments through phenotypic plasticity or genetic variation.On the global scale,we found that human activities and road density positively contribute to the G.quadriradiata expansion in mountainous areas.Field surveys in China revealed significant elevational differences in the seed dispersal traits of G.quadriradiata,with higher-elevation populations exhibiting lower dispersal ability and generally lower genetic diversity.Under common conditions,high-elevation populations showed higher leaf mass ratio but lower root mass ratio and reproductive allocation.This suggests that high-elevation environments create a barrier to dispersal for G.quadriradiata,and that G.quadriradiata has adapted phenotypically to these conditions.Our study indicates that the elevational invasion pattern of G.quadriradiata is shaped by multiple factors,particularly human activities and phenotypic adaptability.In addition,our finding that G.quadriradiata invasion at high elevations is not constrained by low genetic diversity indicates that monitoring and management of G.quadriradiata in mountainous areas should be strengthened.
基金financially supported by the National Natural Science Foundation of China (32071978)the National Key Research and Development Program of China (2022YFD2300901 and 2022YFD2300905)。
文摘High temperature stress (HT) significantly reduces maize yield by impairing starch accumulation in kernels.However,the mechanism by which HT affects starch synthesis remains controversial-whether through reduced assimilate supply or direct inhibition on kernel metabolism.To clarify these mechanisms,a heat-sensitive maize hybrid,Xianyu 335 (XY),was exposed to 30℃/20℃ (maximum/minimum temperature,control) and 40℃/30℃ for seven consecutive days during the seed setting stage.Synchronous pollination (SP),apical pollination (AP),and shading treatments were applied to manipulate the inherent source–sink ratio in maize plants.Results showed that apical kernel weight decreased by 11.9%under 40℃ in the SP treatment.The ^(13)C content,starch accumulation,and cell-wall invertase (CWIN) activity also declined by 15.9,36.7,and 16.4%,respectively,under HT.In the shading treatment,40℃/30℃ caused even greater reductions in^(13)C content,starch accumulation,and CWIN activity due to diminished assimilate supply.Conversely,in the AP treatment,starch content and CWIN activity increased by 22.0 and 18.5%,respectively,under 40℃/30℃,resulting in kernel weight and ^(13)C content similar to those in SP and shading treatments regardless of temperature.Consistent with apical kernels under AP,HT did not negatively affect middle kernels in either SP or shading treatments,as kernel weight and starch content remained unchanged under HT.Although all kernels were exposed to the same HT or control environment,their responses varied a lot.The impaired starch synthesis in apical kernels under HT was rescued by increasing carbon supply via AP treatment.The contrasting performance among middle kernels,apical kernels under AP,and apical kernels under SP or shading indicates that reduced carbon supply is a critical factor underlying inhibited starch accumulation.Our findings provide a theoretical basis for further understanding kernel abortion under HT.
文摘In the seeding operations in order to mitigate the climatic changes or to intervene beneficently on the precipitations process, it is very important to know the roll of the critical radius size of the cloud drops formation and its posterior evolution. In the seeding operations programs, the fundament is to determinate the critical radius in order to obtain efficient results. So, it must consider （a） the critical radius size necessary in order to get the better results; （b） the atmospheric conditions that determine it. In order to get a methodology to calculate the critical radius in each atmospheric condition, the present work has been developed. And with them, it can estimate the nuclei size necessary in order to assure good seeding. The authors had obtained approximate values that were good enough to the goals.
文摘Objective:To evaluate the nursing effects of auricular seed therapy combined with Chinese herbal poultice treatment for patients with lumbar disc herniation(LDH).Method:A total of 80 patients with LDH were randomly divided into two groups.The combined group received auricular acupuncture with seed application combined with Chinese herbal poultice therapy,while the reference group received auricular acupuncture with seed application alone.The therapeutic outcomes of both groups were compared.Results:The combined intervention group demonstrated decreased sleep quality scores,reduced pain neurotransmitter levels,and improved lumbar spine function scores post-intervention,with significant intergroup differences(p<0.05).Conclusion:Auricular seed therapy combined with Chinese herbal poultice care effectively improves sleep quality and lumbar spine function while alleviating pain perception in patients with lumbar disc herniation,indicating superior therapeutic efficacy.
基金supported by the Jilin Science and Technology Development Program,China (20240602032RC)the Jilin Agricultural Science and Technology Innovation Project,China (CXGC2024ZD001)+1 种基金the Jilin Agricultural Science and Technology Innovation Project,China (CXGC2024ZY012)the Jilin Province Development and Reform Commission-Project for Improving the Independent Innovation Capacity of Major Grain Crops,China (2024C002)。
文摘Emerging and powerful genome editing tools,particularly CRISPR/Cas9,are facilitating functional genomics research and accelerating crop improvement(Jiang et al.2021;Cao et al.2023;Chen C et al.2023;Liu et al.2023a).However,the detection and screening of transgenic lines remain major bottlenecks,being time-consuming,labor-intensive,and inefficient during transformation and subsequent mutation identification.A simple and efficient visual marker system plays a critical role in addressing these challenges.Recent studies demonstrated that the GmW1 and RUBY reporter systems were used to obtain visual transgenic soybean(Glycine max) plants(Chen L et al.2023;Chen et al.2024).
