Seed aging tolerance during storage is generally an important trait for crop production, yet the role of small auxin-up RNA genes in conferring seed aging tolerance is largely unknown in rice. In this study, one small...Seed aging tolerance during storage is generally an important trait for crop production, yet the role of small auxin-up RNA genes in conferring seed aging tolerance is largely unknown in rice. In this study, one small auxin-up RNA gene, OsSAUR33, was found to be involved in the regulation of seed aging tolerance in rice. The expression of OsSAUR33 was significantly induced in aged seeds compared with unaged seeds during the seed germination phase. Accordingly, the disruption of OsSAUR33 significantly reduced seed vigor compared to the wild type(WT) in response to natural storage or artificial aging treatments. The rice OsSAUR33 gene promotes the vigor of aged seeds by enhancing their reactive oxygen species(ROS) level during seed germination, and the accumulation of ROS was significantly delayed in the aged seeds of Ossaur33 mutants in comparison with WT during seed germination. Hydrogen peroxide(H_(2)O_(2)) treatments promoted the vigor of aged seeds in various rice varieties. Our results provide timely theoretical and technical insights for the trait improvement of seed aging tolerance in rice.展开更多
Reasons causing or accelerating seed aging are mainly damage of mem- branes, DNA and proteins, decline of protein synthesis capacity and excessive ac- cumulation of reactive oxygen species. With the application of nat...Reasons causing or accelerating seed aging are mainly damage of mem- branes, DNA and proteins, decline of protein synthesis capacity and excessive ac- cumulation of reactive oxygen species. With the application of natural aging or artifi- cial aging methods, it was reported that quantitative trait loci （QTLs） of seed stora- bility in rice were widely distributed on the chromosomes except the 10th chromo- some. In this paper, we reviewed the progresses in the research on physiological- biochemical and genetic mechanisms of seed aging, and analyzed the existing problems and developing prospect in molecular breeding of rice with improved seed storability, in order to provide reference for the basic research and genetic improve- ment of rice seed storabUity.展开更多
β-Aminobutyric acid(BABA)is a physiologically active plant compound that has not been extensively studied.It has been shown to increase resistance to biotic and abiotic stress factors and enhance seed germination in ...β-Aminobutyric acid(BABA)is a physiologically active plant compound that has not been extensively studied.It has been shown to increase resistance to biotic and abiotic stress factors and enhance seed germination in certain plant species.However,its effects on cereal grains with low germination rates have not yet been studied.This study investigated the effects of BABA on the germination of aged triticale seeds,the metabolite content of seedlings,and the state of their antioxidant systems.The study found that a three-hour treatment of seeds in BABA solutions at concentrations ranging from 0.1 to 1.0 mM increased germination energy and germination(by 10%–14%)and enhanced the accumulation of shoot and root biomass(by 17%–26%).Additionally,amylase activity increased in the grains,and the accumulation of osmolytes(sugars and proline)increased in the shoots.The content of anthocyanins in shoots increased by almost twofold,and the activity of antioxidant enzymes(superoxide dismutase,catalase,and guaiacol peroxidase)increased by approximately 20%–30%.Simultaneously,BABA seed priming caused a noticeable decrease in the levels of hydrogen peroxide and lipid peroxidation products in the shoots of seedlings.The conclusion was made that the use of BABA as a bioregulator has the potential to enhance the germination of seeds with low sowing qualities.This is due to the ability of BABA to activate the metabolism of reserve substances in the grain and prevent the development of oxidative stress.展开更多
Seed viability is an essential feature for genetic resource conservation as well as sustainable crop production.Long-term storage induces seed viability deterioration or seed aging,accompanied by the accumulation of t...Seed viability is an essential feature for genetic resource conservation as well as sustainable crop production.Long-term storage induces seed viability deterioration or seed aging,accompanied by the accumulation of toxic reactive oxygen species(ROS)to suppress seed germination.Controlled deterioration treatment(CDT)is a gen-eral approach for mimicking seed aging.The transcription factor ANAC089 was previously reported to modulate seed primary germination.In this study,we evaluated the ability of ANAC089 to control seed viability during aging.Compared with that in the wild-type line,the mutation of ANAC089 significantly increased H_(2)O_(2),thereby reducing seed viability after CDT,while the overexpression of ANAC089 reduced H_(2)O_(2) and improved seed long-evity,indicating a critical role for ANAC089 in maintaining seed viability through H_(2)O_(2) signaling.A series of stu-dies have shown that ANAC089 targets and negatively regulates the level of ABI5,an important transmitter of abscisic acid(ABA)signals,to affect seed viability after CDT.Furthermore,ABI5 negatively regulated the expres-sion of VTC2,which is involved in the biosynthesis of the antioxidant ascorbic acid and H_(2)O_(2) scavenging.As a result,ANAC089 attenuates the generation of H_(2)O_(2),thereby enhancing seed viability through the ABI5-VTC2 module during the seed aging process.Taken together,our results reveal a novel mechanism by which ANAC089 enhances seed viability by coordinating ABI5 and VTC2 expression,ultimately preventing the overac-cumulation of H_(2)O_(2),which would have led to reduced seed viability.展开更多
Nano-particles have several interesting properties which are not shown in bulk materials because of their high ratio of surface area to volume. FeCo alloy nano-particles with soft magnetic properties are demanded for ...Nano-particles have several interesting properties which are not shown in bulk materials because of their high ratio of surface area to volume. FeCo alloy nano-particles with soft magnetic properties are demanded for various applications such as optics, eletronics and magnetics. Through the polyol method, highly purified particles with mono-dispersibility in various sizes can be produced and used as high-tech functional materials. In this study, the magnetic characteristics of FeCo alloy with Ag seed added in the production process of mono-dispersed nano-particles through the polyol method were investigated.展开更多
基金supported by the Key-Area Research and Development Program of Guangdong Province,China(2022B0202060006)the National Natural Science Foundation of China(32201838,32272157,32172052,and 31971995)+2 种基金the Natural Science Foundation of Guangdong Province,China(2023A1515012052)the Project of Sanya Yazhou Bay Science and Technology City,China(SCKJJYRC-2022-87)the Double First-class Discipline Promotion Project,China(2021B10564001)
文摘Seed aging tolerance during storage is generally an important trait for crop production, yet the role of small auxin-up RNA genes in conferring seed aging tolerance is largely unknown in rice. In this study, one small auxin-up RNA gene, OsSAUR33, was found to be involved in the regulation of seed aging tolerance in rice. The expression of OsSAUR33 was significantly induced in aged seeds compared with unaged seeds during the seed germination phase. Accordingly, the disruption of OsSAUR33 significantly reduced seed vigor compared to the wild type(WT) in response to natural storage or artificial aging treatments. The rice OsSAUR33 gene promotes the vigor of aged seeds by enhancing their reactive oxygen species(ROS) level during seed germination, and the accumulation of ROS was significantly delayed in the aged seeds of Ossaur33 mutants in comparison with WT during seed germination. Hydrogen peroxide(H_(2)O_(2)) treatments promoted the vigor of aged seeds in various rice varieties. Our results provide timely theoretical and technical insights for the trait improvement of seed aging tolerance in rice.
基金Supported by Natural Science Foundation of Hainan Province(20163129)
文摘Reasons causing or accelerating seed aging are mainly damage of mem- branes, DNA and proteins, decline of protein synthesis capacity and excessive ac- cumulation of reactive oxygen species. With the application of natural aging or artifi- cial aging methods, it was reported that quantitative trait loci （QTLs） of seed stora- bility in rice were widely distributed on the chromosomes except the 10th chromo- some. In this paper, we reviewed the progresses in the research on physiological- biochemical and genetic mechanisms of seed aging, and analyzed the existing problems and developing prospect in molecular breeding of rice with improved seed storability, in order to provide reference for the basic research and genetic improve- ment of rice seed storabUity.
基金support from the project of the Ministry of Education and Science of Ukraine 2-24-26 BO Development of Measures to Ensure Sustainable Productivity of Agrophytocenoses under the Influence of Abiotic and Biotic Stress Factors,state registration No.0124U000457.
文摘β-Aminobutyric acid(BABA)is a physiologically active plant compound that has not been extensively studied.It has been shown to increase resistance to biotic and abiotic stress factors and enhance seed germination in certain plant species.However,its effects on cereal grains with low germination rates have not yet been studied.This study investigated the effects of BABA on the germination of aged triticale seeds,the metabolite content of seedlings,and the state of their antioxidant systems.The study found that a three-hour treatment of seeds in BABA solutions at concentrations ranging from 0.1 to 1.0 mM increased germination energy and germination(by 10%–14%)and enhanced the accumulation of shoot and root biomass(by 17%–26%).Additionally,amylase activity increased in the grains,and the accumulation of osmolytes(sugars and proline)increased in the shoots.The content of anthocyanins in shoots increased by almost twofold,and the activity of antioxidant enzymes(superoxide dismutase,catalase,and guaiacol peroxidase)increased by approximately 20%–30%.Simultaneously,BABA seed priming caused a noticeable decrease in the levels of hydrogen peroxide and lipid peroxidation products in the shoots of seedlings.The conclusion was made that the use of BABA as a bioregulator has the potential to enhance the germination of seeds with low sowing qualities.This is due to the ability of BABA to activate the metabolism of reserve substances in the grain and prevent the development of oxidative stress.
基金supported by the National Natural Science Foundation of China(31970289 to X.H.and 32170562 to P.L.).
文摘Seed viability is an essential feature for genetic resource conservation as well as sustainable crop production.Long-term storage induces seed viability deterioration or seed aging,accompanied by the accumulation of toxic reactive oxygen species(ROS)to suppress seed germination.Controlled deterioration treatment(CDT)is a gen-eral approach for mimicking seed aging.The transcription factor ANAC089 was previously reported to modulate seed primary germination.In this study,we evaluated the ability of ANAC089 to control seed viability during aging.Compared with that in the wild-type line,the mutation of ANAC089 significantly increased H_(2)O_(2),thereby reducing seed viability after CDT,while the overexpression of ANAC089 reduced H_(2)O_(2) and improved seed long-evity,indicating a critical role for ANAC089 in maintaining seed viability through H_(2)O_(2) signaling.A series of stu-dies have shown that ANAC089 targets and negatively regulates the level of ABI5,an important transmitter of abscisic acid(ABA)signals,to affect seed viability after CDT.Furthermore,ABI5 negatively regulated the expres-sion of VTC2,which is involved in the biosynthesis of the antioxidant ascorbic acid and H_(2)O_(2) scavenging.As a result,ANAC089 attenuates the generation of H_(2)O_(2),thereby enhancing seed viability through the ABI5-VTC2 module during the seed aging process.Taken together,our results reveal a novel mechanism by which ANAC089 enhances seed viability by coordinating ABI5 and VTC2 expression,ultimately preventing the overac-cumulation of H_(2)O_(2),which would have led to reduced seed viability.
基金supported by the MKE(The Min-istry of Knowledge Economy),Korea,the ITRC(Information Technology Research Center) Support program supervised by the NIPA(National IT Industry Promotion Agency),No.NIPA-2010-C1090-1021-0015
文摘Nano-particles have several interesting properties which are not shown in bulk materials because of their high ratio of surface area to volume. FeCo alloy nano-particles with soft magnetic properties are demanded for various applications such as optics, eletronics and magnetics. Through the polyol method, highly purified particles with mono-dispersibility in various sizes can be produced and used as high-tech functional materials. In this study, the magnetic characteristics of FeCo alloy with Ag seed added in the production process of mono-dispersed nano-particles through the polyol method were investigated.
