To overcome the limitations of low efficiency and reliance on manual processes in the measurement of geometric parameters for bridge prefabricated components,a method based on deep learning and computer vision is deve...To overcome the limitations of low efficiency and reliance on manual processes in the measurement of geometric parameters for bridge prefabricated components,a method based on deep learning and computer vision is developed to identify the geometric parameters.The study utilizes a common precast element for highway bridges as the research subject.First,edge feature points of the bridge component section are extracted from images of the precast component cross-sections by combining the Canny operator with mathematical morphology.Subsequently,a deep learning model is developed to identify the geometric parameters of the precast components using the extracted edge coordinates from the images as input and the predefined control parameters of the bridge section as output.A dataset is generated by varying the control parameters and noise levels for model training.Finally,field measurements are conducted to validate the accuracy of the developed method.The results indicate that the developed method effectively identifies the geometric parameters of bridge precast components,with an error rate maintained within 5%.展开更多
Tissue clearing combined with high-resolution confocal imaging is a cutting-edge approach for dissecting the three-dimensional(3D)architecture of tissues and deciphering cellular spatial interactions under physiologic...Tissue clearing combined with high-resolution confocal imaging is a cutting-edge approach for dissecting the three-dimensional(3D)architecture of tissues and deciphering cellular spatial interactions under physiological and pathological conditions.Deciphering the spatial interaction of leptin receptor-expressing(LepR^(+))stromal cells with other compartments in the bone marrow is crucial for a deeper understanding of the stem cell niche and the skeletal tissue.In this study,we introduce an optimized protocol for the 3D analysis of skeletal tissues,enabling the visualization of hematopoietic and stromal cells,especially LepR+stromal cells,within optically cleared bone hemisections.Our method preserves the 3D tissue architecture and is extendable to other hematopoietic sites such as calvaria and vertebrae.The protocol entails tissue fixation,decalcification,and cryosectioning to reveal the marrow cavity.Completed within approximately 12 days,this process yields highly transparent tissues that maintain genetically encoded or antibody-stained fluorescent signals.The bone hemisections are compatible with diverse antibody labeling strategies.Confocal microscopy of these transparent samples allows for qualitative and quantitative image analysis using Aivia or Bitplane Imaris software,assessing a spectrum of parameters.With proper storage,the fluorescent signal in the stained and cleared bone hemisections remains intact for at least 2–3 months.This protocol is robust,straightforward to implement,and highly reproducible,offering a valuable tool for tissue architecture and cellular interaction studies.展开更多
Here,we report the spin-orbit state-resolved differential cross sections(DCSs)for the prototype barrierless reaction S(^(1)D)+HD.Both product channels,namely H+SD(^(2)Π_(3/2,1/2))and D+SH(^(2)Π_(3/2,1/2)),were measu...Here,we report the spin-orbit state-resolved differential cross sections(DCSs)for the prototype barrierless reaction S(^(1)D)+HD.Both product channels,namely H+SD(^(2)Π_(3/2,1/2))and D+SH(^(2)Π_(3/2,1/2)),were measured by high-resolution crossed molecular beam experiments.The DCSs of the two product channels show an overall forward-backward symmetry,in accordance with statistical model predictions.However,the DCSs for different spin-orbit manifolds show different preferences in forward or backward scattering directions at the same collision energies.This study reveals that,even though the title reaction proceeds via the long-lived complex mechanism,the spin-orbit coupling effects in the product channels play an important role in the reaction process.展开更多
Inelastic collisions are the dominant cause of energy loss in radiotherapy.In the energy range around the Bragg peak,single ionization(SI)and single-electron capture(SC)are the primary inelastic collisions that lead t...Inelastic collisions are the dominant cause of energy loss in radiotherapy.In the energy range around the Bragg peak,single ionization(SI)and single-electron capture(SC)are the primary inelastic collisions that lead to energy loss.This study employs the classical trajectory Monte Carlo method to study the SI and SC processes of H_(2)O molecules using He^(2+) and C^(6+) projectiles in the energy range of 10 keV/u to 10 MeV/u.The total cross sections,single differential cross sections,impact parameter dependence of SI and SC,and fragmentation cross sections were investigated.Results illustrate that the cross section for SI is the highest when the projectile energy is close to the Bragg peak energy.When the projectile energy is below the Bragg peak energy,the ionized electrons in the forward direction dominate,and the removal of electrons can be associated with large impact parameters.As the projectile energy increases,the emission angle of the electrons gradually transitions from small angles(60°~120°)to large angles(60°~120°),and the removal of electrons is associated with small impact parameters.The energy distributions of the ionized electron are similar when the projectile energy is equal to,below or above the Bragg peak energy.The fragmentation cross sections after SI and SC in the energy range around the Bragg peak were also estimated.展开更多
The direct and dissociative ionizations of oxygen molecule are investigated experimen-tally by electron collision with energies from 350 eV to 8000 eV.The absolute ionization cross sections for the product ions(O_(2)^...The direct and dissociative ionizations of oxygen molecule are investigated experimen-tally by electron collision with energies from 350 eV to 8000 eV.The absolute ionization cross sections for the product ions(O_(2)^(2+),O_(2)^(2+)O^(+),O^(2+),and their total)and two Coulomb explosion channels(O^(+)+O^(+)and O^(2+)+O^(+))are obtained by putting the data of O^(2+)on the scale of Ar+from O_(2)and Ar gases mixed with a fixed relative flow ratio of 1:1.The experimental errors are assessed by taking uncertainties of various factors into account.The present absolute cross sections are well consistent with the previous data in the overlapped energy range below 1000 eV.展开更多
The neutron capture cross section for^(165)Ho was measured at the backstreaming white neutron beam line(Back-n)of the China Spallation Neutron Source(CSNS)using total energy detection systems,composed of a set of four...The neutron capture cross section for^(165)Ho was measured at the backstreaming white neutron beam line(Back-n)of the China Spallation Neutron Source(CSNS)using total energy detection systems,composed of a set of four C_(6)D_(6)scintillator detectors coupled with pulse height weighting techniques.The resonance parameters were extracted using the multilevel multichannel R-matrix code SAMMY to fit the measured capture yields of the^(165)Ho(n,γ)reaction in the neutron energy range below100 eV.Subsequently,the resonance region’s capture cross sections were reconstructed based on the obtained parameters.Furthermore,the unresolved resonance average cross section of the^(165)Ho(n,γ)reaction was determined relative to that of the standard sample^(197)Au within the neutron energy range of 2 keV to 1 MeV.The experimental data were compared with the recommended nuclear data from the ENDF/B-VIII.0 library,as well as with results of calculations performed using the TALYS-1.9 code.The comparison revealed agreement between the measured^(165)Ho(n,γ)cross sections and these data.The present results are crucial for evaluating the^(165)Ho neutron capture cross section and thus enhance the quality of evaluated nuclear data libraries.They provide valuable guidance for nuclear theoretical models and nuclear astrophysical studies.展开更多
Taking state-controlled section of Xuzhou Peiyan river as an example,the current water quality was monitored,and the cause of over water quality standard was analyzed.The results showed that the pollutants of Peiyan r...Taking state-controlled section of Xuzhou Peiyan river as an example,the current water quality was monitored,and the cause of over water quality standard was analyzed.The results showed that the pollutants of Peiyan river were mainly from the urban river segment,and the immediate cause of over standard was tributary pollutants in urban river segment with the runoff into rivers.So we should strengthen the maintenance of drainage control and gate-han,accelerate the construction of urban wastewater treatment facilities and supporting pipe network,promote rural decentralized sewage treatment,prevent and control livestock and poultry pollution,pesticides and fertilizers pollution,and intercept non-point source pollution by using eco-technology.展开更多
This paper describes a new method for simulation of the cross section shape of log. The self-developed MQK3102 log shape recognizing machine was used to acquire the finite discrete sampling points on the cross section...This paper describes a new method for simulation of the cross section shape of log. The self-developed MQK3102 log shape recognizing machine was used to acquire the finite discrete sampling points on the cross section of log and those points were fitted with the quadratic B-spline parametric curve. This method can clearly stimulate the real shape of the log cross section and is characterized by limited sampling points and high speed computing. The computed result of the previous curve does not affect the next one, which may avoid the graphic distortion caused by the accumulative error. The method can be used to simulate the whole body shape of log approximately by sampling the cross sections along the length direction of log, thus providing a reference model for optimum saw cutting of log.展开更多
[Objective] This study aimed to establish a new method for preparing paraffin sections of cattle eyebal s. [Method] The conventional method was used to prepare paraffin sections for cattle eyebal s in the control and ...[Objective] This study aimed to establish a new method for preparing paraffin sections of cattle eyebal s. [Method] The conventional method was used to prepare paraffin sections for cattle eyebal s in the control and a new method termed＆quot;opening a window on cornea and refixation＆quot; was used to prepare paraffin sections for cattle eyebal s in the treatment group. [Result] After the prepared specimens in the treatment group were fixed, it could be macroscopical y observed that retina and choroid were closely connected, with detachment occurring at a smal portion be-tween the two. According to the paraffin sections, it was microscopical y observed that the continuity of trabecular meshwork was intact, as wel as the continuity be-tween different layers of eyebal wal , without detachment between them, no retinal detachment, no shrinkage of each layer of tissue cells. [Conclusion] This study pro-vides a foundation for the basic research and pathological study of eyebal s.展开更多
With the rapid development of modern electronic technology,the demand for high-performance microwave absorption materials has increased dramatically.In order to meet this demand,the electrospinning of FeNiCo/carbon na...With the rapid development of modern electronic technology,the demand for high-performance microwave absorption materials has increased dramatically.In order to meet this demand,the electrospinning of FeNiCo/carbon nanofiber(FeNiCo/CNF)composites with excellent microwave absorption properties was developed,and their potential as high frequency microwave absorption materials was evaluated.Experiment showed that FeNiCo/CNFs achieve a minimum reflection loss(RL_(min))of−55.5 dB with a matching thickness of only 1.6 mm.Microstructure analysis and electromagnetic parameter testing showed that the excellent microwave absorbing properties were mainly due to the combined effect of the network structure of carbon nanofibers and the FeNiCo alloy.This interaction promotes multiple reflections and the efficient absorption of microwaves.Computer simulation also showed that the FeNiCo/CNF composites produce an excellent radar cross-section reduction in typical radar operating frequency bands,which validates their potential application in stealth technology.This is a new concept in the development of high-performance microwave absorption materials.展开更多
Knowing the precise relationship between fuel loading and reactivity is essential for guiding reactor criticality extrapolation and online refueling in molten salt reactors(MSRs).This study aims to explore and explain...Knowing the precise relationship between fuel loading and reactivity is essential for guiding reactor criticality extrapolation and online refueling in molten salt reactors(MSRs).This study aims to explore and explain the linear relationship between reactivity and the reciprocal of uranium concentration in thermal-spectrum MSRs.By applying neutron balance theory,we analyzed the neutron absorption cross sections of various nuclides in single-lattice models with varying fuel concentrations.Our findings reveal a simple linear correlation between reactivity and the reciprocal of uranium concentration,which can be explained from the perspective of nuclear reaction cross sections that adhere to the 1/v law in the thermal neutron spectrum.Furthermore,we identified that the neutron absorption single-group cross sections of structural materials and carrier salts exhibit an approximately linear relationship with the fission single-group cross section of ^(235) U;similarly,the reciprocal of ^(235)U’s fission cross section exhibits an approximately linear relationship with uranium concentration.This linear relationship deviates as the volume fraction of molten salt increases,due to a greater proportion of neutrons being captured in the resonance energy spectrum.However,it remains valid for molten salt volume fractions up to 25%and demonstrates broad applicability in the physical design and operation of thermal molten salt reactors.展开更多
In recent years,terbium radioisotopes have been investigated for their potential therapeutic and diagnostic applications in nuclear medicine.This study aimed to investigate the production of ^(152) Tb and ^(155) Tb by...In recent years,terbium radioisotopes have been investigated for their potential therapeutic and diagnostic applications in nuclear medicine.This study aimed to investigate the production of ^(152) Tb and ^(155) Tb by alpha-induced reactions in detail,with a specific focus on determining the optimum production parameters and testing existing nuclear models.Given the limited number of experiments conducted on reactions related to terbium isotope production,it is necessary to perform theoretical calculations of cross sections over a wide energy range to gain a detailed understanding of terbium isotope production.To achieve this objective,the cross sections of the ^(151)Eu(α,n)^(154) Tb reactions were calculated up to 60 MeV using the TALYS computer code with 432 different combinations of optical model parameters,level density,and strength function models.The theoretical reaction cross-section results were compared with the experimental results in the literature.The best input parameters were determined using the Threshold Logic Unit method,and these parameters were used in all isotope production calculations.Once the optimal model combination was determined,the total activity production and isotopic fraction of ^(152) Tb and ^(155) Tb isotopes were calculated in detail for beam energies of 17–50 MeV,different irradiation times,and varying ^(151) Eu and ^(153) Eu target thicknesses.展开更多
Wide-field mesoscopy provides the capabilities of cortex-wide field of view(FOV),cellular resolution and high frame rate for neuronal imaging in the mouse brain.However,inherent background fluorescence degrades the im...Wide-field mesoscopy provides the capabilities of cortex-wide field of view(FOV),cellular resolution and high frame rate for neuronal imaging in the mouse brain.However,inherent background fluorescence degrades the image quality and hinders neuronal signal extraction.To address this problem,we first introduce a cortex-wide,high-resolution lineillumination mesoscope with a moving slit designed for in vivo mouse brain imaging.This system achieves a 6.6×6.6 mm FOV,microscale cellular resolution,a high frame rate of 10 Hz,as well as the background rejection ability.Furthermore,we integrated patterned illumination into the system to enhance the background suppression.Experimental results show that the proposed system successfully captures neurodynamics in the living mouse brain.Compared with conventional wide-field mesoscopes,the cortex-wide patterned line-illumination mesoscope(PLIM)achieves a threefold increase in the signal-to-background ratio(SBR).With patterned illumination integrated,the SBR enhancement further reaches four-anda-half-fold.展开更多
Based on the generalized reduced R-matrix theory,the R-matrix analysis code(RAC program)was used to analyze the experimental data of all the nuclear reaction channels related to the 5 He system.The current calculation...Based on the generalized reduced R-matrix theory,the R-matrix analysis code(RAC program)was used to analyze the experimental data of all the nuclear reaction channels related to the 5 He system.The current calculations provide accurate and reliable evaluation data and are in good agreement with the experimental data.In this study,self-consistent evaluation data for each reaction were obtained using multi-channel and multi-energy fitting.In particular,the error propagation theory of generalized least squares was used to determine the error of the evaluation data and the covariance matrix of the integral cross section.This R-matrix analysis for the 5 He system has three features.First,for the first time,the error in the evaluation data of the T(d,n)^(4)He reaction cross section and the covariance matrix of the integral cross section are provided.Second,we used only one set of R-matrix parameters to depict the reaction cross section of each reaction channel of the 5 He system for the entire energy region in our work.Third,in this evaluation,we considered some of the latest measured experimental data,especially after 2000.The T(d,n)^(4)He reaction cross section at 0.1 MeV and below was carefully studied.The effect of different energy levels in T(d,n)^(4)He was analyzed,with the energy levels 3/2^(+)making a major contribution to the cross section,and the role of the S-wave and P-wave from 3/2~-determines the lean forward trend of the angular distributions at 0.01–0.1 MeV.展开更多
The evaporation residual cross sections(ERCSs)of these reactions were calculated by using^(144)Sm,^(160,164)Dy,^(165)Ho,^(166)Er,^(169)Tm,^(171,174)Yb,^(175)Lu,^(176-180)Hf,^(181)Ta,^(180,182)W and^(187)Re targets wit...The evaporation residual cross sections(ERCSs)of these reactions were calculated by using^(144)Sm,^(160,164)Dy,^(165)Ho,^(166)Er,^(169)Tm,^(171,174)Yb,^(175)Lu,^(176-180)Hf,^(181)Ta,^(180,182)W and^(187)Re targets with^(40)Ar projectiles in the theoretical framework of the dinuclear system(DNS)model.The de-excitation process of the compound nucleus was theoretically calculated using two different statistical models,namely the statistical model 1 and statistical model 2(GEMINI++model).The calculated ERCSs were also compared with the experimental data.The ERCSs of synthesizing new proton-rich nuclides were investigated based on the fusion evaporation reaction.Predictions were made for the ERCSs of new isotopes of Pu,Cm and Bk in the heavy nuclei region,while the new isotopes of Ds,Cn and Fl are predicted in the superheavy nuclei region of Z≥104.展开更多
Let M be a compact n-manifold of positive sectional curvature.We will review classical results on the fundamental group of M,a motivation on the c(n)-cyclic conjecture that the fundamental group of M contains a cyclic...Let M be a compact n-manifold of positive sectional curvature.We will review classical results on the fundamental group of M,a motivation on the c(n)-cyclic conjecture that the fundamental group of M contains a cyclic subgroup of index bounded above by c(n),a constant depending only on n,and we will survey partial results(up to date)on the c(n)-cyclic conjecture.展开更多
Generalised reduced masses with a set of equations governing the three relative motions between two of 3-bodies in their gravitational field are established,of which the dynamic characteristics of 3-body dynamics,fund...Generalised reduced masses with a set of equations governing the three relative motions between two of 3-bodies in their gravitational field are established,of which the dynamic characteristics of 3-body dynamics,fundamental bases of this paper,are revealed.Based on these findings,an equivalent system is developed,which is a 2-body system with its total mass,constant angular momentum,kinetic and potential energies same as the total ones of three relative motions,so that it can be solved using the well-known theory of the 2-body system.From the solution of an equivalent system with the revealed characteristics of three relative motions,the general theoretical solutions of the 3-body system are obtained in the curve-integration forms along the orbits in the imaged radial motion space.The possible periodical orbits with generalised Kepler’s law are presented.Following the description and mathematical demonstrations of the proposed methods,the examples including Euler’s/Lagrange’s problems,and a reported numerical one are solved to validate the proposed methods.The methods derived from the 3-body system are extended to N-body problems.展开更多
Climate model prediction has been improved by enhancing model resolution as well as the implementation of sophisticated physical parameterization and refinement of data assimilation systems[section 6.1 in Wang et al.(...Climate model prediction has been improved by enhancing model resolution as well as the implementation of sophisticated physical parameterization and refinement of data assimilation systems[section 6.1 in Wang et al.(2025)].In relation to seasonal forecasting and climate projection in the East Asian summer monsoon season,proper simulation of the seasonal migration of rain bands by models is a challenging and limiting factor[section 7.1 in Wang et al.(2025)].展开更多
The single electron capture processes in Si^(3,4+)+He collisions have been investigated theoretically employing the two-center atomic orbital close-coupling method in the energy range 0.01-100 keV/u.Total and state-se...The single electron capture processes in Si^(3,4+)+He collisions have been investigated theoretically employing the two-center atomic orbital close-coupling method in the energy range 0.01-100 keV/u.Total and state-selective electron capture cross sections for the dominant and subdominant reaction channels are calculated and compared with the available experimental and theoretical data.For the total charge transfer cross sections,the present results show better agreements with the available experimental data than the other theoretical ones in the overlapping energy region for both collision systems.For the state-selective cross sections,the present results for 3s and 3p states are in general agreement with the previous MOCC results in the low energy region for both collision systems.Furthermore,the cross sections for electron captured to the 3d,4l and 5l(l=0,1,...,n-1)states of Si^(2+)and Si^(3+)ions are first provided in a broad energy region in our work.These results are useful for the investigations in astrophysics.The datasets presented in this paper,including the total and state-selective electron capture cross sections of Si^(3,4+)+He collisions in 0.01-100 ke V/u,are openly available at https://doi.org/10.57760/sciencedb.j00113.00257.展开更多
AIM:To compare the performance of three commercially available anti-human epidermalgrowth factor receptor 2(HER2)antibodies in whole-tissue sections and tissue microarrays(TMAs)of a series of gastric tumors.METHODS:We...AIM:To compare the performance of three commercially available anti-human epidermalgrowth factor receptor 2(HER2)antibodies in whole-tissue sections and tissue microarrays(TMAs)of a series of gastric tumors.METHODS:We present a comparative analysis of three anti-HER2 antibodies(HercepTest,4B5 and SP3)using TMA and whole-tissue sections prepared from the same paraffin blocks of 199 gastric adenocarcinomas operated upon between January 2004 and December2008 at a Brazilian cancer hospital.The data on the patients’age,sex,the anatomical location of the tumor and the Lauren’s histological classification were collected from clinical and pathological records.The immunohistochemical(IHC)results were examined by two pathologists and the cases were classified as positive(3+),equivocal(2+)and negative(0 or 1+),according to the criteria of the IHC scoring system of gastric cancer.TMAs and whole-tissue sections were evaluated separately and independently.All cases yielding discordant IHC results and/or scored as 2+were subjected to dual-color in situ hybridization in order to determine the final HER2 status.Besides determining the sensitivity and predictive value for HER2-positive status,we measured the accuracy of each antibody by calculating the area under the receiver operating characteristic(ROC)curve.The agreement between the results obtained using the TMAs and those obtained using the whole-tissue sections was assessed by means of Kappa coefficient.RESULTS:Intratumoral heterogeneity of HER2 expression was observed with all antibodies.HER2-positive expression(3+)in the whole-tissue sections was observed in 23 cases(11.6%)using the 4B5 antibody,in 18 cases(9.1%)using the SP3 antibody and in 10 cases(5.1%)using the HercepTest antibody.In the TMAs,11 positive cases(5.6%)were identified using SP3 antibody,9(4.6%)using the 4B5 antibody and 6(3%)using the HercepTest antibody.The sensitivity using whole-tissue sections and TMA,respectively,was 95.2%and 42.9%with 4B5,90.5%and 66.7%with SP3 and 47.6%and42.9%with HercepTest.The accuracy,calculated from the area under the ROC curve,using whole-tissue sections and TMA,respectively,was 0.91 and 0.79 by 4B5,0.86 and 0.80 by SP3 and 0.73 and 0.71 by HercepTest.The concordance of the results obtained using wholetissue sections and TMA was 97.4%(Kappa 0.75)using HercepTest,85.6%(Kappa 0.56)using SP3 and 84.1%(Kappa 0.38)using 4B5.CONCLUSION:The use of the 4B5 antibody on wholetissue sections was the most accurate IHC method for evaluating HER2 expression in gastric adenocarcinoma.展开更多
基金The National Natural Science Foundation of China(No.52338011,52378291)Young Elite Scientists Sponsorship Program by CAST(No.2022-2024QNRC0101).
文摘To overcome the limitations of low efficiency and reliance on manual processes in the measurement of geometric parameters for bridge prefabricated components,a method based on deep learning and computer vision is developed to identify the geometric parameters.The study utilizes a common precast element for highway bridges as the research subject.First,edge feature points of the bridge component section are extracted from images of the precast component cross-sections by combining the Canny operator with mathematical morphology.Subsequently,a deep learning model is developed to identify the geometric parameters of the precast components using the extracted edge coordinates from the images as input and the predefined control parameters of the bridge section as output.A dataset is generated by varying the control parameters and noise levels for model training.Finally,field measurements are conducted to validate the accuracy of the developed method.The results indicate that the developed method effectively identifies the geometric parameters of bridge precast components,with an error rate maintained within 5%.
基金National Natural Science Foundation of China(grant number 82272563 to B.S.)National Science and Technology Major Project of the Ministry of Science and Technology of China(grant number 2023ZD0501202 to B.S.)+4 种基金institutional grants allocated to the National Institute of Biological Sciences,Beijing(NIBS)from the Chinese Ministry of Science and Technology,Beijing Municipal Commission of Science and Technology,and Tsinghua Universitythe support from China Pharmaceutical University(grant number 3150140001 to S.F.)National Natural Science Foundation of China(grant numbers 82203653 to S.F.,82371957 to L.W.,and 82371956 to X.C.)Beijing Municipal Public Welfare Development and Reform Pilot Project for Medical Research Institutes(grant number JYY2023-8 to X.C.)Research Grants Council of University Grants Committee Hong Kong(grant numbers 14113723,14108720,14121721,14202920,N_CUHK472/22,C7030-18G,T13-402/17-N,and AoE/M-402/20)。
文摘Tissue clearing combined with high-resolution confocal imaging is a cutting-edge approach for dissecting the three-dimensional(3D)architecture of tissues and deciphering cellular spatial interactions under physiological and pathological conditions.Deciphering the spatial interaction of leptin receptor-expressing(LepR^(+))stromal cells with other compartments in the bone marrow is crucial for a deeper understanding of the stem cell niche and the skeletal tissue.In this study,we introduce an optimized protocol for the 3D analysis of skeletal tissues,enabling the visualization of hematopoietic and stromal cells,especially LepR+stromal cells,within optically cleared bone hemisections.Our method preserves the 3D tissue architecture and is extendable to other hematopoietic sites such as calvaria and vertebrae.The protocol entails tissue fixation,decalcification,and cryosectioning to reveal the marrow cavity.Completed within approximately 12 days,this process yields highly transparent tissues that maintain genetically encoded or antibody-stained fluorescent signals.The bone hemisections are compatible with diverse antibody labeling strategies.Confocal microscopy of these transparent samples allows for qualitative and quantitative image analysis using Aivia or Bitplane Imaris software,assessing a spectrum of parameters.With proper storage,the fluorescent signal in the stained and cleared bone hemisections remains intact for at least 2–3 months.This protocol is robust,straightforward to implement,and highly reproducible,offering a valuable tool for tissue architecture and cellular interaction studies.
基金supported by the National Natural Science Foundation of China(Nos.92476207,22288201)the Chinese Academy of Sciences(No.XBD0970202)+2 种基金the Innovation Program for Quantum Science and Technology(No.2021ZD0303300)the Shenzhen Science and Technology Innovation Committee(No.20220814164755002)the Guangdong Innovative&Entrepreneurial Research Team Program(Nos.2019ZT08L455,2019JC01X091).
文摘Here,we report the spin-orbit state-resolved differential cross sections(DCSs)for the prototype barrierless reaction S(^(1)D)+HD.Both product channels,namely H+SD(^(2)Π_(3/2,1/2))and D+SH(^(2)Π_(3/2,1/2)),were measured by high-resolution crossed molecular beam experiments.The DCSs of the two product channels show an overall forward-backward symmetry,in accordance with statistical model predictions.However,the DCSs for different spin-orbit manifolds show different preferences in forward or backward scattering directions at the same collision energies.This study reveals that,even though the title reaction proceeds via the long-lived complex mechanism,the spin-orbit coupling effects in the product channels play an important role in the reaction process.
基金supported in part by the National Natural Science Foundation of China(Nos.12105327 and 11775108)the Hunan Provincial Innovation Foundation For Postgraduate(No.QL20220210)the Advanced Energy Science and Technology Guangdong Laboratory.
文摘Inelastic collisions are the dominant cause of energy loss in radiotherapy.In the energy range around the Bragg peak,single ionization(SI)and single-electron capture(SC)are the primary inelastic collisions that lead to energy loss.This study employs the classical trajectory Monte Carlo method to study the SI and SC processes of H_(2)O molecules using He^(2+) and C^(6+) projectiles in the energy range of 10 keV/u to 10 MeV/u.The total cross sections,single differential cross sections,impact parameter dependence of SI and SC,and fragmentation cross sections were investigated.Results illustrate that the cross section for SI is the highest when the projectile energy is close to the Bragg peak energy.When the projectile energy is below the Bragg peak energy,the ionized electrons in the forward direction dominate,and the removal of electrons can be associated with large impact parameters.As the projectile energy increases,the emission angle of the electrons gradually transitions from small angles(60°~120°)to large angles(60°~120°),and the removal of electrons is associated with small impact parameters.The energy distributions of the ionized electron are similar when the projectile energy is equal to,below or above the Bragg peak energy.The fragmentation cross sections after SI and SC in the energy range around the Bragg peak were also estimated.
基金supported by the National Key Research and Development Program of China(No.2022YFA1602502)the National Natural Science Foundation of China(No.12127804).
文摘The direct and dissociative ionizations of oxygen molecule are investigated experimen-tally by electron collision with energies from 350 eV to 8000 eV.The absolute ionization cross sections for the product ions(O_(2)^(2+),O_(2)^(2+)O^(+),O^(2+),and their total)and two Coulomb explosion channels(O^(+)+O^(+)and O^(2+)+O^(+))are obtained by putting the data of O^(2+)on the scale of Ar+from O_(2)and Ar gases mixed with a fixed relative flow ratio of 1:1.The experimental errors are assessed by taking uncertainties of various factors into account.The present absolute cross sections are well consistent with the previous data in the overlapped energy range below 1000 eV.
基金supported by the National Natural Science Foundation of China(Nos.12465024,12365018,U2032146)Inner Mongolia National Science Foundation(Nos.2024ZD23,2024FX30,2023MS01005)+1 种基金Program for Innovative Research Team in Universities of Inner Mongolia Autonomous Region(NMGIRT2217)Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region(NJYT23109)。
文摘The neutron capture cross section for^(165)Ho was measured at the backstreaming white neutron beam line(Back-n)of the China Spallation Neutron Source(CSNS)using total energy detection systems,composed of a set of four C_(6)D_(6)scintillator detectors coupled with pulse height weighting techniques.The resonance parameters were extracted using the multilevel multichannel R-matrix code SAMMY to fit the measured capture yields of the^(165)Ho(n,γ)reaction in the neutron energy range below100 eV.Subsequently,the resonance region’s capture cross sections were reconstructed based on the obtained parameters.Furthermore,the unresolved resonance average cross section of the^(165)Ho(n,γ)reaction was determined relative to that of the standard sample^(197)Au within the neutron energy range of 2 keV to 1 MeV.The experimental data were compared with the recommended nuclear data from the ENDF/B-VIII.0 library,as well as with results of calculations performed using the TALYS-1.9 code.The comparison revealed agreement between the measured^(165)Ho(n,γ)cross sections and these data.The present results are crucial for evaluating the^(165)Ho neutron capture cross section and thus enhance the quality of evaluated nuclear data libraries.They provide valuable guidance for nuclear theoretical models and nuclear astrophysical studies.
文摘Taking state-controlled section of Xuzhou Peiyan river as an example,the current water quality was monitored,and the cause of over water quality standard was analyzed.The results showed that the pollutants of Peiyan river were mainly from the urban river segment,and the immediate cause of over standard was tributary pollutants in urban river segment with the runoff into rivers.So we should strengthen the maintenance of drainage control and gate-han,accelerate the construction of urban wastewater treatment facilities and supporting pipe network,promote rural decentralized sewage treatment,prevent and control livestock and poultry pollution,pesticides and fertilizers pollution,and intercept non-point source pollution by using eco-technology.
基金The research is supported by Project of National Natural Science Foundation of China(30571455)and National "948" Project(2005-4-62)
文摘This paper describes a new method for simulation of the cross section shape of log. The self-developed MQK3102 log shape recognizing machine was used to acquire the finite discrete sampling points on the cross section of log and those points were fitted with the quadratic B-spline parametric curve. This method can clearly stimulate the real shape of the log cross section and is characterized by limited sampling points and high speed computing. The computed result of the previous curve does not affect the next one, which may avoid the graphic distortion caused by the accumulative error. The method can be used to simulate the whole body shape of log approximately by sampling the cross sections along the length direction of log, thus providing a reference model for optimum saw cutting of log.
基金Supported by China Agriculture Research System(CARS-38)~~
文摘[Objective] This study aimed to establish a new method for preparing paraffin sections of cattle eyebal s. [Method] The conventional method was used to prepare paraffin sections for cattle eyebal s in the control and a new method termed＆quot;opening a window on cornea and refixation＆quot; was used to prepare paraffin sections for cattle eyebal s in the treatment group. [Result] After the prepared specimens in the treatment group were fixed, it could be macroscopical y observed that retina and choroid were closely connected, with detachment occurring at a smal portion be-tween the two. According to the paraffin sections, it was microscopical y observed that the continuity of trabecular meshwork was intact, as wel as the continuity be-tween different layers of eyebal wal , without detachment between them, no retinal detachment, no shrinkage of each layer of tissue cells. [Conclusion] This study pro-vides a foundation for the basic research and pathological study of eyebal s.
基金supported by the Natural Science Foundation of Shanxi Province(202203021212205)Shanxi Province Major Science and Technology Special Project‘Jiebang Guashuai’Project(202101120401008)+1 种基金National Natural Science Foundation of China(52371231)Key R&D Program of Shanxi Province(202302040201008).
文摘With the rapid development of modern electronic technology,the demand for high-performance microwave absorption materials has increased dramatically.In order to meet this demand,the electrospinning of FeNiCo/carbon nanofiber(FeNiCo/CNF)composites with excellent microwave absorption properties was developed,and their potential as high frequency microwave absorption materials was evaluated.Experiment showed that FeNiCo/CNFs achieve a minimum reflection loss(RL_(min))of−55.5 dB with a matching thickness of only 1.6 mm.Microstructure analysis and electromagnetic parameter testing showed that the excellent microwave absorbing properties were mainly due to the combined effect of the network structure of carbon nanofibers and the FeNiCo alloy.This interaction promotes multiple reflections and the efficient absorption of microwaves.Computer simulation also showed that the FeNiCo/CNF composites produce an excellent radar cross-section reduction in typical radar operating frequency bands,which validates their potential application in stealth technology.This is a new concept in the development of high-performance microwave absorption materials.
基金supported by the Youth Innovation Promotion Association of the Chinese Academy of Sciences(No.2020261)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA02010000)the Young Potential Program of the Shanghai Institute of Applied Physics,Chinese Academy of Sciences(No.SINAP-YXJH-202412)。
文摘Knowing the precise relationship between fuel loading and reactivity is essential for guiding reactor criticality extrapolation and online refueling in molten salt reactors(MSRs).This study aims to explore and explain the linear relationship between reactivity and the reciprocal of uranium concentration in thermal-spectrum MSRs.By applying neutron balance theory,we analyzed the neutron absorption cross sections of various nuclides in single-lattice models with varying fuel concentrations.Our findings reveal a simple linear correlation between reactivity and the reciprocal of uranium concentration,which can be explained from the perspective of nuclear reaction cross sections that adhere to the 1/v law in the thermal neutron spectrum.Furthermore,we identified that the neutron absorption single-group cross sections of structural materials and carrier salts exhibit an approximately linear relationship with the fission single-group cross section of ^(235) U;similarly,the reciprocal of ^(235)U’s fission cross section exhibits an approximately linear relationship with uranium concentration.This linear relationship deviates as the volume fraction of molten salt increases,due to a greater proportion of neutrons being captured in the resonance energy spectrum.However,it remains valid for molten salt volume fractions up to 25%and demonstrates broad applicability in the physical design and operation of thermal molten salt reactors.
文摘In recent years,terbium radioisotopes have been investigated for their potential therapeutic and diagnostic applications in nuclear medicine.This study aimed to investigate the production of ^(152) Tb and ^(155) Tb by alpha-induced reactions in detail,with a specific focus on determining the optimum production parameters and testing existing nuclear models.Given the limited number of experiments conducted on reactions related to terbium isotope production,it is necessary to perform theoretical calculations of cross sections over a wide energy range to gain a detailed understanding of terbium isotope production.To achieve this objective,the cross sections of the ^(151)Eu(α,n)^(154) Tb reactions were calculated up to 60 MeV using the TALYS computer code with 432 different combinations of optical model parameters,level density,and strength function models.The theoretical reaction cross-section results were compared with the experimental results in the literature.The best input parameters were determined using the Threshold Logic Unit method,and these parameters were used in all isotope production calculations.Once the optimal model combination was determined,the total activity production and isotopic fraction of ^(152) Tb and ^(155) Tb isotopes were calculated in detail for beam energies of 17–50 MeV,different irradiation times,and varying ^(151) Eu and ^(153) Eu target thicknesses.
基金support from the National Natural Science Foundation of China(Grant No.61971256)。
文摘Wide-field mesoscopy provides the capabilities of cortex-wide field of view(FOV),cellular resolution and high frame rate for neuronal imaging in the mouse brain.However,inherent background fluorescence degrades the image quality and hinders neuronal signal extraction.To address this problem,we first introduce a cortex-wide,high-resolution lineillumination mesoscope with a moving slit designed for in vivo mouse brain imaging.This system achieves a 6.6×6.6 mm FOV,microscale cellular resolution,a high frame rate of 10 Hz,as well as the background rejection ability.Furthermore,we integrated patterned illumination into the system to enhance the background suppression.Experimental results show that the proposed system successfully captures neurodynamics in the living mouse brain.Compared with conventional wide-field mesoscopes,the cortex-wide patterned line-illumination mesoscope(PLIM)achieves a threefold increase in the signal-to-background ratio(SBR).With patterned illumination integrated,the SBR enhancement further reaches four-anda-half-fold.
基金supported by Science Challenge Project(No.TZ20180001)。
文摘Based on the generalized reduced R-matrix theory,the R-matrix analysis code(RAC program)was used to analyze the experimental data of all the nuclear reaction channels related to the 5 He system.The current calculations provide accurate and reliable evaluation data and are in good agreement with the experimental data.In this study,self-consistent evaluation data for each reaction were obtained using multi-channel and multi-energy fitting.In particular,the error propagation theory of generalized least squares was used to determine the error of the evaluation data and the covariance matrix of the integral cross section.This R-matrix analysis for the 5 He system has three features.First,for the first time,the error in the evaluation data of the T(d,n)^(4)He reaction cross section and the covariance matrix of the integral cross section are provided.Second,we used only one set of R-matrix parameters to depict the reaction cross section of each reaction channel of the 5 He system for the entire energy region in our work.Third,in this evaluation,we considered some of the latest measured experimental data,especially after 2000.The T(d,n)^(4)He reaction cross section at 0.1 MeV and below was carefully studied.The effect of different energy levels in T(d,n)^(4)He was analyzed,with the energy levels 3/2^(+)making a major contribution to the cross section,and the role of the S-wave and P-wave from 3/2~-determines the lean forward trend of the angular distributions at 0.01–0.1 MeV.
基金supported by the National Natural Science Foundation of China(Nos.12175064 and U2167203)Hunan Outstanding Youth Science Foundation(No.2022JJ10031)。
文摘The evaporation residual cross sections(ERCSs)of these reactions were calculated by using^(144)Sm,^(160,164)Dy,^(165)Ho,^(166)Er,^(169)Tm,^(171,174)Yb,^(175)Lu,^(176-180)Hf,^(181)Ta,^(180,182)W and^(187)Re targets with^(40)Ar projectiles in the theoretical framework of the dinuclear system(DNS)model.The de-excitation process of the compound nucleus was theoretically calculated using two different statistical models,namely the statistical model 1 and statistical model 2(GEMINI++model).The calculated ERCSs were also compared with the experimental data.The ERCSs of synthesizing new proton-rich nuclides were investigated based on the fusion evaporation reaction.Predictions were made for the ERCSs of new isotopes of Pu,Cm and Bk in the heavy nuclei region,while the new isotopes of Ds,Cn and Fl are predicted in the superheavy nuclei region of Z≥104.
文摘Let M be a compact n-manifold of positive sectional curvature.We will review classical results on the fundamental group of M,a motivation on the c(n)-cyclic conjecture that the fundamental group of M contains a cyclic subgroup of index bounded above by c(n),a constant depending only on n,and we will survey partial results(up to date)on the c(n)-cyclic conjecture.
文摘Generalised reduced masses with a set of equations governing the three relative motions between two of 3-bodies in their gravitational field are established,of which the dynamic characteristics of 3-body dynamics,fundamental bases of this paper,are revealed.Based on these findings,an equivalent system is developed,which is a 2-body system with its total mass,constant angular momentum,kinetic and potential energies same as the total ones of three relative motions,so that it can be solved using the well-known theory of the 2-body system.From the solution of an equivalent system with the revealed characteristics of three relative motions,the general theoretical solutions of the 3-body system are obtained in the curve-integration forms along the orbits in the imaged radial motion space.The possible periodical orbits with generalised Kepler’s law are presented.Following the description and mathematical demonstrations of the proposed methods,the examples including Euler’s/Lagrange’s problems,and a reported numerical one are solved to validate the proposed methods.The methods derived from the 3-body system are extended to N-body problems.
文摘Climate model prediction has been improved by enhancing model resolution as well as the implementation of sophisticated physical parameterization and refinement of data assimilation systems[section 6.1 in Wang et al.(2025)].In relation to seasonal forecasting and climate projection in the East Asian summer monsoon season,proper simulation of the seasonal migration of rain bands by models is a challenging and limiting factor[section 7.1 in Wang et al.(2025)].
基金supported by the National Key Research and Development Program of China(Grant No.2022YFA1602504)the National Natural Science Foundation of China(Grant Nos.12274040 and U2430208)。
文摘The single electron capture processes in Si^(3,4+)+He collisions have been investigated theoretically employing the two-center atomic orbital close-coupling method in the energy range 0.01-100 keV/u.Total and state-selective electron capture cross sections for the dominant and subdominant reaction channels are calculated and compared with the available experimental and theoretical data.For the total charge transfer cross sections,the present results show better agreements with the available experimental data than the other theoretical ones in the overlapping energy region for both collision systems.For the state-selective cross sections,the present results for 3s and 3p states are in general agreement with the previous MOCC results in the low energy region for both collision systems.Furthermore,the cross sections for electron captured to the 3d,4l and 5l(l=0,1,...,n-1)states of Si^(2+)and Si^(3+)ions are first provided in a broad energy region in our work.These results are useful for the investigations in astrophysics.The datasets presented in this paper,including the total and state-selective electron capture cross sections of Si^(3,4+)+He collisions in 0.01-100 ke V/u,are openly available at https://doi.org/10.57760/sciencedb.j00113.00257.
文摘AIM:To compare the performance of three commercially available anti-human epidermalgrowth factor receptor 2(HER2)antibodies in whole-tissue sections and tissue microarrays(TMAs)of a series of gastric tumors.METHODS:We present a comparative analysis of three anti-HER2 antibodies(HercepTest,4B5 and SP3)using TMA and whole-tissue sections prepared from the same paraffin blocks of 199 gastric adenocarcinomas operated upon between January 2004 and December2008 at a Brazilian cancer hospital.The data on the patients’age,sex,the anatomical location of the tumor and the Lauren’s histological classification were collected from clinical and pathological records.The immunohistochemical(IHC)results were examined by two pathologists and the cases were classified as positive(3+),equivocal(2+)and negative(0 or 1+),according to the criteria of the IHC scoring system of gastric cancer.TMAs and whole-tissue sections were evaluated separately and independently.All cases yielding discordant IHC results and/or scored as 2+were subjected to dual-color in situ hybridization in order to determine the final HER2 status.Besides determining the sensitivity and predictive value for HER2-positive status,we measured the accuracy of each antibody by calculating the area under the receiver operating characteristic(ROC)curve.The agreement between the results obtained using the TMAs and those obtained using the whole-tissue sections was assessed by means of Kappa coefficient.RESULTS:Intratumoral heterogeneity of HER2 expression was observed with all antibodies.HER2-positive expression(3+)in the whole-tissue sections was observed in 23 cases(11.6%)using the 4B5 antibody,in 18 cases(9.1%)using the SP3 antibody and in 10 cases(5.1%)using the HercepTest antibody.In the TMAs,11 positive cases(5.6%)were identified using SP3 antibody,9(4.6%)using the 4B5 antibody and 6(3%)using the HercepTest antibody.The sensitivity using whole-tissue sections and TMA,respectively,was 95.2%and 42.9%with 4B5,90.5%and 66.7%with SP3 and 47.6%and42.9%with HercepTest.The accuracy,calculated from the area under the ROC curve,using whole-tissue sections and TMA,respectively,was 0.91 and 0.79 by 4B5,0.86 and 0.80 by SP3 and 0.73 and 0.71 by HercepTest.The concordance of the results obtained using wholetissue sections and TMA was 97.4%(Kappa 0.75)using HercepTest,85.6%(Kappa 0.56)using SP3 and 84.1%(Kappa 0.38)using 4B5.CONCLUSION:The use of the 4B5 antibody on wholetissue sections was the most accurate IHC method for evaluating HER2 expression in gastric adenocarcinoma.
