A new Fe-SOD gene from a native Chinese tobacco germplasm namely HZNH has been successfully cloned and expressed. Full-length cDNA sequences of the Fe-SOD gene was obtained by employing the 5′ and 3′end RACE method ...A new Fe-SOD gene from a native Chinese tobacco germplasm namely HZNH has been successfully cloned and expressed. Full-length cDNA sequences of the Fe-SOD gene was obtained by employing the 5′ and 3′end RACE method from the HZNH′s cDNA library. The full sequence was 1145 bp in length, including 170 bp of 5′untranslated region, 288 bp of 3′untranslated region and 687 bp of coding region. The coding region encoded a peptide of 228 amino acid residues, in which there was a signal peptide with 26 amino acids and a mature peptide of 202 amino acids. The full-length cDNA sequence was compared with all other reported plants’ Fe-SOD genes’. The result of Blast analysis showed that they shared high homology(>80%) ,the highest one was to N. plumbaginifolia’s with the homology as high as 97.69%. This cDNA was constructed into the prokaryotic expression vector, pQE30a/FeSOD, and transformed into E.coli M15 which was induced with IPTG. SDS-PAGE showed that 27 kD proteins was expressed. The soluble proteins showed the Fe-SOD enzyme activities on PAGE-based isozyme spectrum indicating that this expressed soluble protein is indeed the Fe-SOD enzyme.展开更多
With the rapid development of China's agricultural production and the improvement of people's living standards and health awareness,people's demands for food quality and nutrition have become increasingly ...With the rapid development of China's agricultural production and the improvement of people's living standards and health awareness,people's demands for food quality and nutrition have become increasingly strong. Agricultural products that meet various functional needs such as nutrition,health care and safety are increasingly favored by the general public. Se,one of the trace elements necessary for the human body,has begun to be applied and developed in medicine and other life sciences. Developing Se-enriched agricultural relying on Guangxi's unique Se-enriched soil resources is of great significance to enhance people's quality of life,increase the added value of agricultural products,and promote the transformation and upgrading of modern agriculture in the region. Starting with the development status and advantages of industrial development of Se-enriched agriculture in Guangxi,the main problems existing in the current industrial development of Se-enriched agriculture in Guangxi were summarized,and specific countermeasures were put forward in this paper,so as to provide theoretical support for the development of Se-enriched agriculture in Guangxi.展开更多
This study was conducted to investigate the effect of Se-enriched lactobacillus on antibody of Newcastle Disease Virus(NDV)of broilers.One thousand day-old AA broiler chickens were selected from a chicken farm of Jing...This study was conducted to investigate the effect of Se-enriched lactobacillus on antibody of Newcastle Disease Virus(NDV)of broilers.One thousand day-old AA broiler chickens were selected from a chicken farm of Jinghai County,and the chickens were divided randomly into two groups,which respectively had five repeat groups with 100 chickens each,one named basal diet group,and the other named Se-enriched lactobacillus group.Corn soybean meal diet was selected for basal diet group,while 2%se-enriched lactobacillus was added into corn soybean meal diet for the other group.The method of feeding was free diet,drinking,and routine immunization program was adopted.This test period was 42 d.Two chickens were selected randomly in each repeat for slaughtering and collecting serums on the 14th,28th and 42nd d.Assessments on the effect of Se-enriched lactobacillus in antibody titer of NDV of broilers were made by hemagglutination-inhibition test.The results indicated that antibody titer of NDV was increased significantly by Se-enriched lactobacillus.展开更多
文摘A new Fe-SOD gene from a native Chinese tobacco germplasm namely HZNH has been successfully cloned and expressed. Full-length cDNA sequences of the Fe-SOD gene was obtained by employing the 5′ and 3′end RACE method from the HZNH′s cDNA library. The full sequence was 1145 bp in length, including 170 bp of 5′untranslated region, 288 bp of 3′untranslated region and 687 bp of coding region. The coding region encoded a peptide of 228 amino acid residues, in which there was a signal peptide with 26 amino acids and a mature peptide of 202 amino acids. The full-length cDNA sequence was compared with all other reported plants’ Fe-SOD genes’. The result of Blast analysis showed that they shared high homology(>80%) ,the highest one was to N. plumbaginifolia’s with the homology as high as 97.69%. This cDNA was constructed into the prokaryotic expression vector, pQE30a/FeSOD, and transformed into E.coli M15 which was induced with IPTG. SDS-PAGE showed that 27 kD proteins was expressed. The soluble proteins showed the Fe-SOD enzyme activities on PAGE-based isozyme spectrum indicating that this expressed soluble protein is indeed the Fe-SOD enzyme.
基金Supported by Science and Technology Plan Project of Guangxi Zhuang Autonomous Region(GK-AA17202037,GK-AB16380164,2015GXNSFAA139101)Science and Technology Project of Xixiangtang,Nanning(201710304)
文摘With the rapid development of China's agricultural production and the improvement of people's living standards and health awareness,people's demands for food quality and nutrition have become increasingly strong. Agricultural products that meet various functional needs such as nutrition,health care and safety are increasingly favored by the general public. Se,one of the trace elements necessary for the human body,has begun to be applied and developed in medicine and other life sciences. Developing Se-enriched agricultural relying on Guangxi's unique Se-enriched soil resources is of great significance to enhance people's quality of life,increase the added value of agricultural products,and promote the transformation and upgrading of modern agriculture in the region. Starting with the development status and advantages of industrial development of Se-enriched agriculture in Guangxi,the main problems existing in the current industrial development of Se-enriched agriculture in Guangxi were summarized,and specific countermeasures were put forward in this paper,so as to provide theoretical support for the development of Se-enriched agriculture in Guangxi.
基金National Natural Science Foundation of China(31101867)Project of Tianjin Agricultural University Affiliated Animal Hospital(ZH004903)。
文摘This study was conducted to investigate the effect of Se-enriched lactobacillus on antibody of Newcastle Disease Virus(NDV)of broilers.One thousand day-old AA broiler chickens were selected from a chicken farm of Jinghai County,and the chickens were divided randomly into two groups,which respectively had five repeat groups with 100 chickens each,one named basal diet group,and the other named Se-enriched lactobacillus group.Corn soybean meal diet was selected for basal diet group,while 2%se-enriched lactobacillus was added into corn soybean meal diet for the other group.The method of feeding was free diet,drinking,and routine immunization program was adopted.This test period was 42 d.Two chickens were selected randomly in each repeat for slaughtering and collecting serums on the 14th,28th and 42nd d.Assessments on the effect of Se-enriched lactobacillus in antibody titer of NDV of broilers were made by hemagglutination-inhibition test.The results indicated that antibody titer of NDV was increased significantly by Se-enriched lactobacillus.
