[Objective] Using molecular biotechnology to clone the sus scrofa GPX2 gene. [Method] Using total RNA of sus scrofa duodenum as template, degenerated primer pairs were designed according to the homology alignment anal...[Objective] Using molecular biotechnology to clone the sus scrofa GPX2 gene. [Method] Using total RNA of sus scrofa duodenum as template, degenerated primer pairs were designed according to the homology alignment analysis of GPX2 gene of human, rat, mouse, dog and cattle. A sus scrofa GPX2 gene sequence of 330 bp was obtained by RT-PCR application method. Primes were designed respectively according to the known sequence, sus scrofa GPX2 gene was isolated and cloned by 3-RACE and 5-RACE method and analyzed the gene sequence. [Result] A mRNA sequence of 924 bp was successfully cloned and isolated in this research. This sequence contained complete 3＇end and had higher sequence homology with human,mouse,cattle and dog GPX2 gene, and there was codon called TGA which encoding Sec on the position of No. 114-116 gene. [Conclusion] Sequence alignment analysis showed that the cloned gene was sus scrofa GPX2 gene （ NCBI GenBank database, the sequence number was D098982）.展开更多
[ Objective] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa, so as to provide references for revealing the physiological functions of this gene. [Method] Wi...[ Objective] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa, so as to provide references for revealing the physiological functions of this gene. [Method] With the mRNA from different tissues as template, RT-PCR amplification was carried out for cloning cDNA of hybrid pig, which was then used for PCR reaction using specific primers; the amplification products were separated by gel electrophoresis and analyzed by sequencing for detecting the distribution pattern of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa. [ Result] H-FABP expressed in all the twelve tissues including subcutaneous fat, abdominal fat, mammary gland, Iongissimus dorsi muscle, dorsal deltoid muscle, heart, liver, spleen, lung, kidney, ileum and duodenum. [ Conclusion] The wide distribution of H-FABP gene suggests that its functions are important and multiple.展开更多
Thyroid peroxidase （TPO） is the key enzyme involved in thyroid hormone synthesis. Several mutations in the TPO gene may affect the normal growth and development of mammals. In this study, the TPO gene was mapped, it...Thyroid peroxidase （TPO） is the key enzyme involved in thyroid hormone synthesis. Several mutations in the TPO gene may affect the normal growth and development of mammals. In this study, the TPO gene was mapped, its expression level was determined in thyroid grand at the age of 1, 20, 45, 60, 90, 120 and 150 days for Jinhua pig, the alternative splicing form was searched and the single nucleotide polymorphism （SNP, A/G642） of TPO gene was analyzed. The results showed that the porcine TPO was mapped to 3q22-27, the expres- sion level of TPO was relatively stable among the various ages and two novel transcript variants in porcine TPO gene were found： the splicing variant TPO-2 lacked exon 8, while TPO-3 lacked exon 8 and exon 14, 15, 16. Moreover, we found that the SNP of A/G642in the fourteenth exon of TPO gene was significantly associated with ham weight （P 〈 0.05）. Our results provided important basis on the regulation and metabolism of the thyroid gland in animals.展开更多
Traceability based on DNA analysis is attracting increasing interest due to the crisis of confidence that consumers show towards the products of animal origin. The present work discusses a genetic traceability system ...Traceability based on DNA analysis is attracting increasing interest due to the crisis of confidence that consumers show towards the products of animal origin. The present work discusses a genetic traceability system of meat and processed products from an historical Tuscan native pig breed, the Cinta Senese. The study is based on a panel of 8 ISAG (International Society for Animal Genetics) DNA microsatellite markers usage done both on pigs and derived products. The SSRs panel allowed us to obtain a unique fingerprint of the individuals to be used as a tracer “downstream” in the processed products. The molecular method used proved that the hams, analyzed just before commercialization, were obtained from Cinta Senese pigs and that the analyzed meat products derived from the Cinta Senese were produced at least with 95% of Cinta Senese meat. In perspective, the molecular testing could be introduced as a voluntarily adopted method for proving intrinsic quality of many regional food products.展开更多
Background:Non-native wild pigs(Sus scrofa)threaten sensitive flora and fauna,cost billions of dollars in economic damage,and pose a significant human–wildlife conflict risk.Despite growing interest in wild pig resea...Background:Non-native wild pigs(Sus scrofa)threaten sensitive flora and fauna,cost billions of dollars in economic damage,and pose a significant human–wildlife conflict risk.Despite growing interest in wild pig research,basic life history information is often lacking throughout their introduced range and particularly in tropical environments.Similar to other large terrestrial mammals,pigs possess the ability to shift their range based on local climatic conditions or resource availability,further complicating management decisions.The objectives of this study were to(i)model the distribution and abundance of wild pigs across two seasons within a single calendar year;(ii)determine the most important environmental variables driving changes in pig distribution and abundance;and(iii)highlight key differences between seasonal models and their potential management implications.These study objectives were achieved using zero-inflated models constructed from abundance data obtained from extensive field surveys and remotely sensed environmental variables.Results:Our models demonstrate a considerable change in distribution and abundance of wild pigs throughout a single calendar year.Rainfall and vegetation height were among the most influential variables for pig distribution during the spring,and distance to adjacent forest and vegetation density were among the most significant for the fall.Further,our seasonal models show that areas of high conservation value may be more vulnerable to threats from wild pigs at certain times throughout the year,which was not captured by more traditional modeling approaches using aggregated data.Conclusions:Our results suggest that(i)wild pigs can considerably shift their range throughout the calendar year,even in tropical environments;(ii)pigs prefer dense forested areas in the presence of either hunting pressure or an abundance of frugivorous plants,but may shift to adjacent areas in the absence of either of these conditions;and(iii)seasonal models provide valuable biological information that would otherwise be missed by common modeling approaches that use aggregated data over many years.These findings highlight the importance of considering biologically relevant time scales that provide key information to better inform management strategies,particularly for species whose ranges include both temperate and tropical environments and thrive in both large continental and small island ecosystems.展开更多
Background Boars undergo physiological and biochemical changes in semen composition as they grow from puberty to sexual maturity.However,comprehensive metabolomic profiles of boar semen remain uncharacterised.Understa...Background Boars undergo physiological and biochemical changes in semen composition as they grow from puberty to sexual maturity.However,comprehensive metabolomic profiles of boar semen remain uncharacterised.Understanding metabolic alterations in semen during this period is important for optimising reproductive performance in breeding programs.The aim of this study was to characterise the semen metabolome as boars mature,utilising an untargeted metabolomic approach.Semen samples were collected from 15 Duroc boars at three developmental ages:~7 months,8.5 months,and 10 months.Sperm and seminal plasma were separated and analysed by hydrophilic interaction and reversed-phase liquid chromatography coupled with mass spectrometry to capture a wide range of metabolites.Results We identified a total of 4,491 features in boar semen,annotating 92 distinct metabolites.Amino acids,peptides and analogues constituted the most abundant components,followed by fatty acid esters.Principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA)showed a clear separation between metabolomic profiles by age groups.PERMANOVA analysis of PCA scores confirmed statistically significant differences(P<0.05)between younger(7 months)and more mature boars(8.5 months and 10 months).Pathway analysis identified porphyrin metabolism,taurine and hypotaurine metabolism,and glycerolipid metabolism as significantly enriched pathways in sperm,while glutathione and nitrogen metabolism were prominently enriched in seminal plasma.Using linear modelling,partial Spearman correlation and random forest analyses,we identified homoisovanillic acid as a key metabolite discriminating age groups in both sperm and seminal plasma.Additionally,L-glutamic acid,decanoyl-L-carnitine and N-(1,3-Thiazol-2-yl)benzenesulfonamide emerged as important sperm metabolites,while glyceric acid,myo-inositol,glycerophosphocholine,and several other compounds were identified as critical seminal plasma metabolites.Conclusion This study provides a detailed characterisation of metabolic changes in Duroc boar semen during the transition from puberty to sexual maturity.Our findings enhance the understanding of reproductive development and could inform strategies to assess sexual maturity in breeding programs.展开更多
文摘[Objective] Using molecular biotechnology to clone the sus scrofa GPX2 gene. [Method] Using total RNA of sus scrofa duodenum as template, degenerated primer pairs were designed according to the homology alignment analysis of GPX2 gene of human, rat, mouse, dog and cattle. A sus scrofa GPX2 gene sequence of 330 bp was obtained by RT-PCR application method. Primes were designed respectively according to the known sequence, sus scrofa GPX2 gene was isolated and cloned by 3-RACE and 5-RACE method and analyzed the gene sequence. [Result] A mRNA sequence of 924 bp was successfully cloned and isolated in this research. This sequence contained complete 3＇end and had higher sequence homology with human,mouse,cattle and dog GPX2 gene, and there was codon called TGA which encoding Sec on the position of No. 114-116 gene. [Conclusion] Sequence alignment analysis showed that the cloned gene was sus scrofa GPX2 gene （ NCBI GenBank database, the sequence number was D098982）.
基金Supported by the National High Technology Research and Develop-ment Program of China(No.2008AA10Z136)the Program of Liaoning Provincial Science-Technology Department(20070567)~~
文摘[ Objective] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa, so as to provide references for revealing the physiological functions of this gene. [Method] With the mRNA from different tissues as template, RT-PCR amplification was carried out for cloning cDNA of hybrid pig, which was then used for PCR reaction using specific primers; the amplification products were separated by gel electrophoresis and analyzed by sequencing for detecting the distribution pattern of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa. [ Result] H-FABP expressed in all the twelve tissues including subcutaneous fat, abdominal fat, mammary gland, Iongissimus dorsi muscle, dorsal deltoid muscle, heart, liver, spleen, lung, kidney, ileum and duodenum. [ Conclusion] The wide distribution of H-FABP gene suggests that its functions are important and multiple.
基金supported by National Basic Research Program of China (973 Program) (No. 2006CB102100)Major Agriculture Research Project of Zhejiang Province (No. 2005C12005-2)National Natural Science Foun-dation of China (No. 30972078)
文摘Thyroid peroxidase （TPO） is the key enzyme involved in thyroid hormone synthesis. Several mutations in the TPO gene may affect the normal growth and development of mammals. In this study, the TPO gene was mapped, its expression level was determined in thyroid grand at the age of 1, 20, 45, 60, 90, 120 and 150 days for Jinhua pig, the alternative splicing form was searched and the single nucleotide polymorphism （SNP, A/G642） of TPO gene was analyzed. The results showed that the porcine TPO was mapped to 3q22-27, the expres- sion level of TPO was relatively stable among the various ages and two novel transcript variants in porcine TPO gene were found： the splicing variant TPO-2 lacked exon 8, while TPO-3 lacked exon 8 and exon 14, 15, 16. Moreover, we found that the SNP of A/G642in the fourteenth exon of TPO gene was significantly associated with ham weight （P 〈 0.05）. Our results provided important basis on the regulation and metabolism of the thyroid gland in animals.
文摘Traceability based on DNA analysis is attracting increasing interest due to the crisis of confidence that consumers show towards the products of animal origin. The present work discusses a genetic traceability system of meat and processed products from an historical Tuscan native pig breed, the Cinta Senese. The study is based on a panel of 8 ISAG (International Society for Animal Genetics) DNA microsatellite markers usage done both on pigs and derived products. The SSRs panel allowed us to obtain a unique fingerprint of the individuals to be used as a tracer “downstream” in the processed products. The molecular method used proved that the hams, analyzed just before commercialization, were obtained from Cinta Senese pigs and that the analyzed meat products derived from the Cinta Senese were produced at least with 95% of Cinta Senese meat. In perspective, the molecular testing could be introduced as a voluntarily adopted method for proving intrinsic quality of many regional food products.
基金supported with a grant from the Hawaiʻi Department of Land and Natural Resource’s Division of Forestry and Wildlife(Grant/Award Number:C01290)。
文摘Background:Non-native wild pigs(Sus scrofa)threaten sensitive flora and fauna,cost billions of dollars in economic damage,and pose a significant human–wildlife conflict risk.Despite growing interest in wild pig research,basic life history information is often lacking throughout their introduced range and particularly in tropical environments.Similar to other large terrestrial mammals,pigs possess the ability to shift their range based on local climatic conditions or resource availability,further complicating management decisions.The objectives of this study were to(i)model the distribution and abundance of wild pigs across two seasons within a single calendar year;(ii)determine the most important environmental variables driving changes in pig distribution and abundance;and(iii)highlight key differences between seasonal models and their potential management implications.These study objectives were achieved using zero-inflated models constructed from abundance data obtained from extensive field surveys and remotely sensed environmental variables.Results:Our models demonstrate a considerable change in distribution and abundance of wild pigs throughout a single calendar year.Rainfall and vegetation height were among the most influential variables for pig distribution during the spring,and distance to adjacent forest and vegetation density were among the most significant for the fall.Further,our seasonal models show that areas of high conservation value may be more vulnerable to threats from wild pigs at certain times throughout the year,which was not captured by more traditional modeling approaches using aggregated data.Conclusions:Our results suggest that(i)wild pigs can considerably shift their range throughout the calendar year,even in tropical environments;(ii)pigs prefer dense forested areas in the presence of either hunting pressure or an abundance of frugivorous plants,but may shift to adjacent areas in the absence of either of these conditions;and(iii)seasonal models provide valuable biological information that would otherwise be missed by common modeling approaches that use aggregated data over many years.These findings highlight the importance of considering biologically relevant time scales that provide key information to better inform management strategies,particularly for species whose ranges include both temperate and tropical environments and thrive in both large continental and small island ecosystems.
基金Open access funding provided by University of Inland Norway The Research Council of Norway provided financial support(grant number 331878).
文摘Background Boars undergo physiological and biochemical changes in semen composition as they grow from puberty to sexual maturity.However,comprehensive metabolomic profiles of boar semen remain uncharacterised.Understanding metabolic alterations in semen during this period is important for optimising reproductive performance in breeding programs.The aim of this study was to characterise the semen metabolome as boars mature,utilising an untargeted metabolomic approach.Semen samples were collected from 15 Duroc boars at three developmental ages:~7 months,8.5 months,and 10 months.Sperm and seminal plasma were separated and analysed by hydrophilic interaction and reversed-phase liquid chromatography coupled with mass spectrometry to capture a wide range of metabolites.Results We identified a total of 4,491 features in boar semen,annotating 92 distinct metabolites.Amino acids,peptides and analogues constituted the most abundant components,followed by fatty acid esters.Principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA)showed a clear separation between metabolomic profiles by age groups.PERMANOVA analysis of PCA scores confirmed statistically significant differences(P<0.05)between younger(7 months)and more mature boars(8.5 months and 10 months).Pathway analysis identified porphyrin metabolism,taurine and hypotaurine metabolism,and glycerolipid metabolism as significantly enriched pathways in sperm,while glutathione and nitrogen metabolism were prominently enriched in seminal plasma.Using linear modelling,partial Spearman correlation and random forest analyses,we identified homoisovanillic acid as a key metabolite discriminating age groups in both sperm and seminal plasma.Additionally,L-glutamic acid,decanoyl-L-carnitine and N-(1,3-Thiazol-2-yl)benzenesulfonamide emerged as important sperm metabolites,while glyceric acid,myo-inositol,glycerophosphocholine,and several other compounds were identified as critical seminal plasma metabolites.Conclusion This study provides a detailed characterisation of metabolic changes in Duroc boar semen during the transition from puberty to sexual maturity.Our findings enhance the understanding of reproductive development and could inform strategies to assess sexual maturity in breeding programs.
