Artificial intelligence(AI)has undergone rapid development and has become increasingly involved in scientific explorations[1].It not only successfully facilitates the efficient completion of previous labor-intensive a...Artificial intelligence(AI)has undergone rapid development and has become increasingly involved in scientific explorations[1].It not only successfully facilitates the efficient completion of previous labor-intensive and time-consuming tasks,including literature review,compound screening,and data analysis,but also guides closed-loop experiments for target functions optimization[2].However,the potential of AI for uncovering new chemical knowledge remains largely untapped[3].展开更多
Engineered microstructures that mimic in vivo tissues have demonstrated great potential for applications in regenerative medicine,drug screening,and cell behavior exploration.However,current methods for engineering mi...Engineered microstructures that mimic in vivo tissues have demonstrated great potential for applications in regenerative medicine,drug screening,and cell behavior exploration.However,current methods for engineering microstructures that mimic the multi-extracellular matrix and multicellular features of natural tissues to realize tissue-mimicking microstructures in vitro remain insufficient.Here,we propose a versatile method for constructing tissue-mimicking heterogeneous microstructures by orderly integration of macroscopic hydrogel exchange,microscopic cell manipulation,and encapsulation modulation.First,various cell-laden hydrogel droplets are manipulated at the millimeter scale using electrowetting on dielectric to achieve efficient hydrogel exchange.Second,the cells are manipulated at the micrometer scale using dielectrophoresis to adjust their density and arrangement within the hydrogel droplets.Third,the photopolymerization of these hydrogel droplets is triggered in designated regions by dynamically modulating the shape and position of the excitation ultraviolet beam.Thus,heterogeneous microstructures with different extracellular matrix geometries and components were constructed,including specific cell densities and patterns.The resulting heterogeneous microstructure supported long-term culture of hepatocytes and fibroblasts with high cell viability(over 90%).Moreover,the density and distribution of the 2 cell types had significant effects on the cell proliferation and urea secretion.We propose that our method can lead to the construction of additional biomimetic heterogeneous microstructures with unprecedented potential for use in future tissue engineering applications.展开更多
Cardiovascular diseases account for ~40% of global deaths annually. This situation has revealed the urgent need forthe investigation and development of corresponding drugs for pathogenesis due to the complexity of res...Cardiovascular diseases account for ~40% of global deaths annually. This situation has revealed the urgent need forthe investigation and development of corresponding drugs for pathogenesis due to the complexity of researchmethods and detection techniques. An in vitro cardiomyocyte model is commonly used for cardiac drug screeningand disease modeling since it can respond to microphysiological environmental variations through mechanoelectricfeedback. Microfluidic platforms are capable of accurate fluid control and integration with analysis and detectiontechniques. Therefore, various microfluidic platforms (i.e., heart-on-a-chip) have been applied for the reconstruction ofthe physiological environment and detection of signals from cardiomyocytes. They have demonstrated advantages inmimicking the cardiovascular structure and function in vitro and in monitoring electromechanical signals. This reviewpresents a summary of the methods and technologies used to monitor the contractility and electrophysiologicalsignals of cardiomyocytes within microfluidic platforms. Then, applications in common cardiac drug screening andcardiovascular disease modeling are presented, followed by design strategies for enhancing physiology studies. Finally,we discuss prospects in the tissue engineering and sensing techniques of microfluidic platforms.展开更多
基金supported by the Start-Up Research Fund of Southeast University(No.4012002405).
文摘Artificial intelligence(AI)has undergone rapid development and has become increasingly involved in scientific explorations[1].It not only successfully facilitates the efficient completion of previous labor-intensive and time-consuming tasks,including literature review,compound screening,and data analysis,but also guides closed-loop experiments for target functions optimization[2].However,the potential of AI for uncovering new chemical knowledge remains largely untapped[3].
基金supported by the National Key Research and Development Program of China under grant 2023YFB4705400the National Natural Science Foundation of China under grant number 62222305,U22A2064+1 种基金the Beijing Natural Science Foundation under grant 4232055the Fundamental Research Program of Shanxi Province 20210302124033.
文摘Engineered microstructures that mimic in vivo tissues have demonstrated great potential for applications in regenerative medicine,drug screening,and cell behavior exploration.However,current methods for engineering microstructures that mimic the multi-extracellular matrix and multicellular features of natural tissues to realize tissue-mimicking microstructures in vitro remain insufficient.Here,we propose a versatile method for constructing tissue-mimicking heterogeneous microstructures by orderly integration of macroscopic hydrogel exchange,microscopic cell manipulation,and encapsulation modulation.First,various cell-laden hydrogel droplets are manipulated at the millimeter scale using electrowetting on dielectric to achieve efficient hydrogel exchange.Second,the cells are manipulated at the micrometer scale using dielectrophoresis to adjust their density and arrangement within the hydrogel droplets.Third,the photopolymerization of these hydrogel droplets is triggered in designated regions by dynamically modulating the shape and position of the excitation ultraviolet beam.Thus,heterogeneous microstructures with different extracellular matrix geometries and components were constructed,including specific cell densities and patterns.The resulting heterogeneous microstructure supported long-term culture of hepatocytes and fibroblasts with high cell viability(over 90%).Moreover,the density and distribution of the 2 cell types had significant effects on the cell proliferation and urea secretion.We propose that our method can lead to the construction of additional biomimetic heterogeneous microstructures with unprecedented potential for use in future tissue engineering applications.
基金supported by the National Natural Science Foundation of China(NO.62371267,62121003)Key R&D Program of Shandong Province(Major innovation project)(2022CXGC020501)+4 种基金Science,Education and Industry Integration Innovation Pilot Project from Qilu University of Technology(Shandong Academy of Sciences)(NO.2022JBZ02-01)Research Leader Studio in Colleges and Universities of Jinan(NO.2021GXRC083)Innovation Team of Organ-on-a-Chip Manufacturing Key Technologies(NO.202333015,Funded by Jinan Science and Technology Bureau)Young Innovative Talents Introduction&Cultivation Program for Colleges and Universities of Shandong Province(Granted by Department of Education of Shandong Province,Sub-Title 1:Innovative Research Team of High-Performance Integrated Device,Sub-Title 2:Innovative Research Team of Advanced Energy Equipment)Shandong Provincial Natural Science Foundation(ZR2023QH405)。
文摘Cardiovascular diseases account for ~40% of global deaths annually. This situation has revealed the urgent need forthe investigation and development of corresponding drugs for pathogenesis due to the complexity of researchmethods and detection techniques. An in vitro cardiomyocyte model is commonly used for cardiac drug screeningand disease modeling since it can respond to microphysiological environmental variations through mechanoelectricfeedback. Microfluidic platforms are capable of accurate fluid control and integration with analysis and detectiontechniques. Therefore, various microfluidic platforms (i.e., heart-on-a-chip) have been applied for the reconstruction ofthe physiological environment and detection of signals from cardiomyocytes. They have demonstrated advantages inmimicking the cardiovascular structure and function in vitro and in monitoring electromechanical signals. This reviewpresents a summary of the methods and technologies used to monitor the contractility and electrophysiologicalsignals of cardiomyocytes within microfluidic platforms. Then, applications in common cardiac drug screening andcardiovascular disease modeling are presented, followed by design strategies for enhancing physiology studies. Finally,we discuss prospects in the tissue engineering and sensing techniques of microfluidic platforms.
