To identify the pathogen responsible for fruit rot disease in Rosa roxburghii Tratt.from Guiding County,Guizhou Province,China,diseased fruit samples were collected.The pathogen was isolated,purified,and identified th...To identify the pathogen responsible for fruit rot disease in Rosa roxburghii Tratt.from Guiding County,Guizhou Province,China,diseased fruit samples were collected.The pathogen was isolated,purified,and identified through morphological,molecular,and pathogenic analyses.Subsequently,its biological characteristicswere evaluated.Furthermore,to determine the agent with the strongest toxicity against the identified pathogen,the antifungal activity of six chemical and biological agents was evaluated through indoor toxicity assays.Finally,Neopestalotiopsis clavispora was identified as the pathogen responsible for fruit rot disease in R.roxburghii Tratt.The diameter of the pathogen grown under different carbon and nitrogen sources,temperatures,and pH values was measured using the crossintersection method.The optimal carbon and nitrogen sources were soluble starch and peptone,respectively.The optimal growth temperature ranged from 25℃ to 30℃,and the optimal growth pH ranged from 4 to 8.The antifungal effects of six agents,including carvacrol 5%aqueous solution and trifloxystrobin–tebuconazole 75%water-dispersible granules,on the mycelial growth rate of N.clavispora were evaluated.All six agents inhibited N.clavispora,with thiophanate–methyl 70%wettable powder showing the strongest antifungal effect and effectively inhibiting mycelial growth even at the lowest concentration.This was followed by difenoconazole–azoxystrobin 48%suspension concentrate,ethylicin 80%emulsifiable concentrate,and trifloxystrobin–tebuconazole 75%WG,with half-maximal effective concentrations of 0.0105,0.0272,and 0.0368 mg/L,respectively.These findings provide a scientific basis for the application of pesticides in the field-based,environmentally friendly control of fruit rot disease in R.roxburghii Tratt.展开更多
MTT Cell Proliferation Assay was used to optimize the concentration of Telomerase Restrictors（TRs） with minimum toxicity to the selected cells. FACSort flow cytometer and Innotest P24 HIV（Human immtmodeficiency Vi...MTT Cell Proliferation Assay was used to optimize the concentration of Telomerase Restrictors（TRs） with minimum toxicity to the selected cells. FACSort flow cytometer and Innotest P24 HIV（Human immtmodeficiency Virus） antigen mAb ELISA Kit were used to investigate the anti-HIV-1 activities of TRs. The results showed that TRs had low cytotoxicity to the PBMC （Peripheral Blood mononuclear cells） and CEM/GFP if the concentration of TRs was at 50μmol/L or below, and the supernatant from PBMC pretreated with SHIV and TR1-001 /TR1-002 could not infect the PBMC, while can infect the C8166 with reduced infectivity, which suggested that the TRs may be one of the novel resources for screening anti-HIV-1 agents.展开更多
The effects of the extracts of 20 Chinese medicinal herbs and an antiviral drug foscarnet on duck hepatitis B virus (DHBV) endogenous DNA polymerase (DNAp) activity were compared. The extracts of P. urinaria showed a ...The effects of the extracts of 20 Chinese medicinal herbs and an antiviral drug foscarnet on duck hepatitis B virus (DHBV) endogenous DNA polymerase (DNAp) activity were compared. The extracts of P. urinaria showed a dose-dependent inhibition on DHBV DNAp. And those of other herbs showed little inhibition effect. Primary duck hepatocyte (PDH) cultures were used for evaluating effects of the extract of P. urinaria, foscarnet and acyclovir (ACV) on DHBV, and all the drugs or the extracts showed inhibition of DHBV DNA replication. Furthermore, in vivo trials were carried out. Peking ducks infected with LJ-76 strain of DHBV were treated with the extract of P. urinaria or ACV and compared with placebo treated control ducks. The treatment results in the loss or reduction of circulating viral DHBV DNA and DHBsAg.展开更多
基金supported by the Science and Technology Support Plan of Guizhou Province(Guizhou Family Combination Support 2022,No.116).
文摘To identify the pathogen responsible for fruit rot disease in Rosa roxburghii Tratt.from Guiding County,Guizhou Province,China,diseased fruit samples were collected.The pathogen was isolated,purified,and identified through morphological,molecular,and pathogenic analyses.Subsequently,its biological characteristicswere evaluated.Furthermore,to determine the agent with the strongest toxicity against the identified pathogen,the antifungal activity of six chemical and biological agents was evaluated through indoor toxicity assays.Finally,Neopestalotiopsis clavispora was identified as the pathogen responsible for fruit rot disease in R.roxburghii Tratt.The diameter of the pathogen grown under different carbon and nitrogen sources,temperatures,and pH values was measured using the crossintersection method.The optimal carbon and nitrogen sources were soluble starch and peptone,respectively.The optimal growth temperature ranged from 25℃ to 30℃,and the optimal growth pH ranged from 4 to 8.The antifungal effects of six agents,including carvacrol 5%aqueous solution and trifloxystrobin–tebuconazole 75%water-dispersible granules,on the mycelial growth rate of N.clavispora were evaluated.All six agents inhibited N.clavispora,with thiophanate–methyl 70%wettable powder showing the strongest antifungal effect and effectively inhibiting mycelial growth even at the lowest concentration.This was followed by difenoconazole–azoxystrobin 48%suspension concentrate,ethylicin 80%emulsifiable concentrate,and trifloxystrobin–tebuconazole 75%WG,with half-maximal effective concentrations of 0.0105,0.0272,and 0.0368 mg/L,respectively.These findings provide a scientific basis for the application of pesticides in the field-based,environmentally friendly control of fruit rot disease in R.roxburghii Tratt.
基金Supported by China Scholarship Counsil(CSC) and Wuhan Chenguang Program of Wuhan City (2003002016-24)
文摘MTT Cell Proliferation Assay was used to optimize the concentration of Telomerase Restrictors（TRs） with minimum toxicity to the selected cells. FACSort flow cytometer and Innotest P24 HIV（Human immtmodeficiency Virus） antigen mAb ELISA Kit were used to investigate the anti-HIV-1 activities of TRs. The results showed that TRs had low cytotoxicity to the PBMC （Peripheral Blood mononuclear cells） and CEM/GFP if the concentration of TRs was at 50μmol/L or below, and the supernatant from PBMC pretreated with SHIV and TR1-001 /TR1-002 could not infect the PBMC, while can infect the C8166 with reduced infectivity, which suggested that the TRs may be one of the novel resources for screening anti-HIV-1 agents.
文摘The effects of the extracts of 20 Chinese medicinal herbs and an antiviral drug foscarnet on duck hepatitis B virus (DHBV) endogenous DNA polymerase (DNAp) activity were compared. The extracts of P. urinaria showed a dose-dependent inhibition on DHBV DNAp. And those of other herbs showed little inhibition effect. Primary duck hepatocyte (PDH) cultures were used for evaluating effects of the extract of P. urinaria, foscarnet and acyclovir (ACV) on DHBV, and all the drugs or the extracts showed inhibition of DHBV DNA replication. Furthermore, in vivo trials were carried out. Peking ducks infected with LJ-76 strain of DHBV were treated with the extract of P. urinaria or ACV and compared with placebo treated control ducks. The treatment results in the loss or reduction of circulating viral DHBV DNA and DHBsAg.
