High-density linkage maps are essential tools for genome analysis of various biological traits. Our developed compact multi-gel system, HEGS （high efficiency genome scanning） is a high-throughput and high-cost-perfo...High-density linkage maps are essential tools for genome analysis of various biological traits. Our developed compact multi-gel system, HEGS （high efficiency genome scanning） is a high-throughput and high-cost-performance electrophoresis apparatus. Using this system, a high-density （average interval 2.3 cM） map with 1 065 AFLP and 63 SSR markers was constructed from recombinant inbred lines of a japonica and indica hybrid in just two months of electrophoreses by a single person. More than 50% of the mapped AFLP markers were commonly polymorphic for several combinations between japonica and indica rice and 15% were applicable for genetically closer crosses between upland and lowland types of japonica rice. This system can be used for rapid analyses of all kinds of markers.展开更多
With the increase of gray scale and flat panel display （FPD） size, subspace bitwise scanning strategy can be replaced traditional scanning method to cut down frame frequency. However, the direct searching strategy ...With the increase of gray scale and flat panel display （FPD） size, subspace bitwise scanning strategy can be replaced traditional scanning method to cut down frame frequency. However, the direct searching strategy （DSS） becomes unfeasible to obtain corresponding high gray scale scanning matrix. Thus, particle swarm optimization （PSO） is introduced to accelerate searching for high gray scale weights scanning matrix （WSM） with its parallelism and global optimization feature. Finally a WSM of 256 gray scales is found out successfully with Matlab, which both gray linearity and scanning efficiency are satisfied.展开更多
文摘High-density linkage maps are essential tools for genome analysis of various biological traits. Our developed compact multi-gel system, HEGS （high efficiency genome scanning） is a high-throughput and high-cost-performance electrophoresis apparatus. Using this system, a high-density （average interval 2.3 cM） map with 1 065 AFLP and 63 SSR markers was constructed from recombinant inbred lines of a japonica and indica hybrid in just two months of electrophoreses by a single person. More than 50% of the mapped AFLP markers were commonly polymorphic for several combinations between japonica and indica rice and 15% were applicable for genetically closer crosses between upland and lowland types of japonica rice. This system can be used for rapid analyses of all kinds of markers.
基金supported by the Shanghai University Innovation Fundation (Grant No.SHUCX102370)
文摘With the increase of gray scale and flat panel display （FPD） size, subspace bitwise scanning strategy can be replaced traditional scanning method to cut down frame frequency. However, the direct searching strategy （DSS） becomes unfeasible to obtain corresponding high gray scale scanning matrix. Thus, particle swarm optimization （PSO） is introduced to accelerate searching for high gray scale weights scanning matrix （WSM） with its parallelism and global optimization feature. Finally a WSM of 256 gray scales is found out successfully with Matlab, which both gray linearity and scanning efficiency are satisfied.
