Cerebral small vessel disease encompasses a group of neurological disorders characterized by injury to small blood vessels,often leading to stroke and dementia.Due to its diverse etiologies and complex pathological me...Cerebral small vessel disease encompasses a group of neurological disorders characterized by injury to small blood vessels,often leading to stroke and dementia.Due to its diverse etiologies and complex pathological mechanisms,preventing and treating cerebral small vessel vasculopathy is challenging.Recent studies have shown that the glymphatic system plays a crucial role in interstitial solute clearance and the maintenance of brain homeostasis.Increasing evidence also suggests that dysfunction in glymphatic clearance is a key factor in the progression of cerebral small vessel disease.This review begins with a comprehensive introduction to the structure,function,and driving factors of the glymphatic system,highlighting its essential role in brain waste clearance.Afterwards,cerebral small vessel disease was reviewed from the perspective of the glymphatic system,after which the mechanisms underlying their correlation were summarized.Glymphatic dysfunction may lead to the accumulation of metabolic waste in the brain,thereby exacerbating the pathological processes associated with cerebral small vessel disease.The review also discussed the direct evidence of glymphatic dysfunction in patients and animal models exhibiting two subtypes of cerebral small vessel disease:arteriolosclerosis-related cerebral small vessel disease and amyloid-related cerebral small vessel disease.Diffusion tensor image analysis along the perivascular space is an important non-invasive tool for assessing the clearance function of the glymphatic system.However,the effectiveness of its parameters needs to be enhanced.Among various nervous system diseases,including cerebral small vessel disease,glymphatic failure may be a common final pathway toward dementia.Overall,this review summarizes prevention and treatment strategies that target glymphatic drainage and will offer valuable insight for developing novel treatments for cerebral small vessel disease.展开更多
Mesenchymal stromal cell transplantation is an effective and promising approach for treating various systemic and diffuse diseases.However,the biological characteristics of transplanted mesenchymal stromal cells in hu...Mesenchymal stromal cell transplantation is an effective and promising approach for treating various systemic and diffuse diseases.However,the biological characteristics of transplanted mesenchymal stromal cells in humans remain unclear,including cell viability,distribution,migration,and fate.Conventional cell tracing methods cannot be used in the clinic.The use of superparamagnetic iron oxide nanoparticles as contrast agents allows for the observation of transplanted cells using magnetic resonance imaging.In 2016,the National Medical Products Administration of China approved a new superparamagnetic iron oxide nanoparticle,Ruicun,for use as a contrast agent in clinical trials.In the present study,an acute hemi-transection spinal cord injury model was established in beagle dogs.The injury was then treated by transplantation of Ruicun-labeled mesenchymal stromal cells.The results indicated that Ruicunlabeled mesenchymal stromal cells repaired damaged spinal cord fibers and partially restored neurological function in animals with acute spinal cord injury.T2*-weighted imaging revealed low signal areas on both sides of the injured spinal cord.The results of quantitative susceptibility mapping with ultrashort echo time sequences indicated that Ruicun-labeled mesenchymal stromal cells persisted stably within the injured spinal cord for over 4 weeks.These findings suggest that magnetic resonance imaging has the potential to effectively track the migration of Ruicun-labeled mesenchymal stromal cells and assess their ability to repair spinal cord injury.展开更多
科学的轨道交通出行模式分析是运营决策优化的重要依据。为挖掘城市轨道交通时空流动特征及其影响机理,提出一种基于非负张量分解的OD客流强度时空分布计算方法,采用融合SHAP归因分析的极端梯度提升树(eXtreme Gradient Boosting,XGBoo...科学的轨道交通出行模式分析是运营决策优化的重要依据。为挖掘城市轨道交通时空流动特征及其影响机理,提出一种基于非负张量分解的OD客流强度时空分布计算方法,采用融合SHAP归因分析的极端梯度提升树(eXtreme Gradient Boosting,XGBoost)对各模式OD客流强度进行拟合预测。使用城市轨道交通AFC(automatic fare collection system,AFC)系统数据,从空间、时段以及出行日3个维度构建3阶客流OD张量,采用交替非负最小二乘法(alternating non negative least squares,ANLS)实现非负CP张量分解。基于张量分解结果,从北京轨道交通344个站点连续1周16266966条出行数据中,提取出晨高峰长距离通勤、早高峰中短通勤、平峰休闲中转出行、晚归出行4种出行模式的时、空分布特征。基于可解释性机器学习模型,对各模式OD客流进行预测。结果表明XGBoost与CatBoost、LightGBM、OLS相比更具优势。根据OD起终点站域环境特征,考虑起终点缓冲区内各类兴趣点(point of interest,POI)数量、小区住户数、房价、人口数量、站点偏离距离以及出行距离等指标,构建OD强度关联指标体系,解释各指标对OD客流强度的正负反馈效应。SHAP归因分析说明,居民更倾向于14站以内的中短途出行,并分别得到了就业类POI数目对晨、早通勤客流正向影响,以及餐饮类POI数目对休闲中转出行客流正向影响的临界阈值。该方法可为轨道交通精细化出行引导和客流组织提供数据支撑,优化城市轨道交通供需平衡及服务水平。展开更多
基金supported by the National Natural Science Foundation of China,No.82274304(to YH)the Major Clinical Study Projects of Shanghai Shenkang Hospital Development Center,No.SHDC2020CR2046B(to YH)Shanghai Municipal Health Commission Talent Plan,No.2022LJ010(to YH).
文摘Cerebral small vessel disease encompasses a group of neurological disorders characterized by injury to small blood vessels,often leading to stroke and dementia.Due to its diverse etiologies and complex pathological mechanisms,preventing and treating cerebral small vessel vasculopathy is challenging.Recent studies have shown that the glymphatic system plays a crucial role in interstitial solute clearance and the maintenance of brain homeostasis.Increasing evidence also suggests that dysfunction in glymphatic clearance is a key factor in the progression of cerebral small vessel disease.This review begins with a comprehensive introduction to the structure,function,and driving factors of the glymphatic system,highlighting its essential role in brain waste clearance.Afterwards,cerebral small vessel disease was reviewed from the perspective of the glymphatic system,after which the mechanisms underlying their correlation were summarized.Glymphatic dysfunction may lead to the accumulation of metabolic waste in the brain,thereby exacerbating the pathological processes associated with cerebral small vessel disease.The review also discussed the direct evidence of glymphatic dysfunction in patients and animal models exhibiting two subtypes of cerebral small vessel disease:arteriolosclerosis-related cerebral small vessel disease and amyloid-related cerebral small vessel disease.Diffusion tensor image analysis along the perivascular space is an important non-invasive tool for assessing the clearance function of the glymphatic system.However,the effectiveness of its parameters needs to be enhanced.Among various nervous system diseases,including cerebral small vessel disease,glymphatic failure may be a common final pathway toward dementia.Overall,this review summarizes prevention and treatment strategies that target glymphatic drainage and will offer valuable insight for developing novel treatments for cerebral small vessel disease.
基金supported by the National Key R&D Program of China,Nos.2017YFA0104302(to NG and XM)and 2017YFA0104304(to BW and ZZ)
文摘Mesenchymal stromal cell transplantation is an effective and promising approach for treating various systemic and diffuse diseases.However,the biological characteristics of transplanted mesenchymal stromal cells in humans remain unclear,including cell viability,distribution,migration,and fate.Conventional cell tracing methods cannot be used in the clinic.The use of superparamagnetic iron oxide nanoparticles as contrast agents allows for the observation of transplanted cells using magnetic resonance imaging.In 2016,the National Medical Products Administration of China approved a new superparamagnetic iron oxide nanoparticle,Ruicun,for use as a contrast agent in clinical trials.In the present study,an acute hemi-transection spinal cord injury model was established in beagle dogs.The injury was then treated by transplantation of Ruicun-labeled mesenchymal stromal cells.The results indicated that Ruicunlabeled mesenchymal stromal cells repaired damaged spinal cord fibers and partially restored neurological function in animals with acute spinal cord injury.T2*-weighted imaging revealed low signal areas on both sides of the injured spinal cord.The results of quantitative susceptibility mapping with ultrashort echo time sequences indicated that Ruicun-labeled mesenchymal stromal cells persisted stably within the injured spinal cord for over 4 weeks.These findings suggest that magnetic resonance imaging has the potential to effectively track the migration of Ruicun-labeled mesenchymal stromal cells and assess their ability to repair spinal cord injury.
文摘科学的轨道交通出行模式分析是运营决策优化的重要依据。为挖掘城市轨道交通时空流动特征及其影响机理,提出一种基于非负张量分解的OD客流强度时空分布计算方法,采用融合SHAP归因分析的极端梯度提升树(eXtreme Gradient Boosting,XGBoost)对各模式OD客流强度进行拟合预测。使用城市轨道交通AFC(automatic fare collection system,AFC)系统数据,从空间、时段以及出行日3个维度构建3阶客流OD张量,采用交替非负最小二乘法(alternating non negative least squares,ANLS)实现非负CP张量分解。基于张量分解结果,从北京轨道交通344个站点连续1周16266966条出行数据中,提取出晨高峰长距离通勤、早高峰中短通勤、平峰休闲中转出行、晚归出行4种出行模式的时、空分布特征。基于可解释性机器学习模型,对各模式OD客流进行预测。结果表明XGBoost与CatBoost、LightGBM、OLS相比更具优势。根据OD起终点站域环境特征,考虑起终点缓冲区内各类兴趣点(point of interest,POI)数量、小区住户数、房价、人口数量、站点偏离距离以及出行距离等指标,构建OD强度关联指标体系,解释各指标对OD客流强度的正负反馈效应。SHAP归因分析说明,居民更倾向于14站以内的中短途出行,并分别得到了就业类POI数目对晨、早通勤客流正向影响,以及餐饮类POI数目对休闲中转出行客流正向影响的临界阈值。该方法可为轨道交通精细化出行引导和客流组织提供数据支撑,优化城市轨道交通供需平衡及服务水平。
