Staphylococcus aureus(S.aureus)is the third most common pathogen causing 10.6%of bacterial foodborne illnesses in China in 2021[1].Heat-stable Staphylococcal Enterotoxins(SEs)produced by S.aureus are the main contribu...Staphylococcus aureus(S.aureus)is the third most common pathogen causing 10.6%of bacterial foodborne illnesses in China in 2021[1].Heat-stable Staphylococcal Enterotoxins(SEs)produced by S.aureus are the main contributors to staphylococcal food poisoning(SFP),causing vomiting,diarrhea,abdominal pain,headache,muscle cramps,and other acute gastroenteritis symptoms.More than 25 SEs and staphylococcal enterotoxin-like toxins(SE/s)have been described and which together comprise a superfamily of pyrogenic toxin superantigens(SAgs)[2].展开更多
The aim of this study was to investigate the synergistic effect of benzyl isothiocyanate(BITC)and resveratrol(RES)on Staphylococcus aureus(S.aureus),the controlled release of the corresponding nanoparticles,and their ...The aim of this study was to investigate the synergistic effect of benzyl isothiocyanate(BITC)and resveratrol(RES)on Staphylococcus aureus(S.aureus),the controlled release of the corresponding nanoparticles,and their application in beef preservation.The synergistic antibacterial effect of BITC and RES on S.aureus was determined using a checkerboard assay and time-to-kill kinetic analysis.The particle size,polydispersity index,zeta potential,Fourier transform infrared spectroscopy and X-ray diffraction results showed that BITC and RES were successfully encapsulated by the soybean protein isolate(SPI).The results of the first-order kinetic model indicated that the regression coefficient(R^(2))values were 0.92-0.98.In addition,the molecular docking results showed that the docking scores of BITC,RES and BITC-RES with SPI ranged from-6.3 to-9.7 kcal/mol,indicating the stability of these nanoparticles.At 36 h,BITC-RES inhibited S.aureus 2.7-3.6 times more than the treatment group alone.SPI-BITC-RES inhibited S.aureus 2.5 times more than did BITC-RES at 48 h.Compared to those in the control group,the numbers of S.aureus in the SPI-BITC and SPI-RES groups decreased by 0.78 CFU/mL and 0.77 CFU/mL,respectively,while the number of S.aureus in the SPI-BITCRES group decreased by 1.54 CFU/mL.The Gompertz model was used to predict the growth of S.aureus in beef treated with BITC,RES,BITC-RES and the corresponding nanoparticles,with R^(2) values greater than 0.90.Therefore,these results suggested that BITC-RES nanoparticles are expected to be useful for food preservation.展开更多
基金supported by the Ministry of Science and Technology of the People’s Republic of China(2022YFD1800400).
文摘Staphylococcus aureus(S.aureus)is the third most common pathogen causing 10.6%of bacterial foodborne illnesses in China in 2021[1].Heat-stable Staphylococcal Enterotoxins(SEs)produced by S.aureus are the main contributors to staphylococcal food poisoning(SFP),causing vomiting,diarrhea,abdominal pain,headache,muscle cramps,and other acute gastroenteritis symptoms.More than 25 SEs and staphylococcal enterotoxin-like toxins(SE/s)have been described and which together comprise a superfamily of pyrogenic toxin superantigens(SAgs)[2].
文摘The aim of this study was to investigate the synergistic effect of benzyl isothiocyanate(BITC)and resveratrol(RES)on Staphylococcus aureus(S.aureus),the controlled release of the corresponding nanoparticles,and their application in beef preservation.The synergistic antibacterial effect of BITC and RES on S.aureus was determined using a checkerboard assay and time-to-kill kinetic analysis.The particle size,polydispersity index,zeta potential,Fourier transform infrared spectroscopy and X-ray diffraction results showed that BITC and RES were successfully encapsulated by the soybean protein isolate(SPI).The results of the first-order kinetic model indicated that the regression coefficient(R^(2))values were 0.92-0.98.In addition,the molecular docking results showed that the docking scores of BITC,RES and BITC-RES with SPI ranged from-6.3 to-9.7 kcal/mol,indicating the stability of these nanoparticles.At 36 h,BITC-RES inhibited S.aureus 2.7-3.6 times more than the treatment group alone.SPI-BITC-RES inhibited S.aureus 2.5 times more than did BITC-RES at 48 h.Compared to those in the control group,the numbers of S.aureus in the SPI-BITC and SPI-RES groups decreased by 0.78 CFU/mL and 0.77 CFU/mL,respectively,while the number of S.aureus in the SPI-BITCRES group decreased by 1.54 CFU/mL.The Gompertz model was used to predict the growth of S.aureus in beef treated with BITC,RES,BITC-RES and the corresponding nanoparticles,with R^(2) values greater than 0.90.Therefore,these results suggested that BITC-RES nanoparticles are expected to be useful for food preservation.
