Sargassum muticum is a globally distributed seaweed with high economic potential,yet its detailed nutritional composition remains undercharacterized.In this study,we analyzed the nutritional profiles of five thallus p...Sargassum muticum is a globally distributed seaweed with high economic potential,yet its detailed nutritional composition remains undercharacterized.In this study,we analyzed the nutritional profiles of five thallus portions,namely apical fronds,middle fronds,basal fronds,receptacles and air bladders,to assess their economic value accordingly.Results showed that middle fronds had the highest crude protein(31.98%)and crude fiber(7.10%)contents,indicating their suitability for protein-rich functional foods or dietary fiber supplements.Receptacles exhibited the highest levels of crude lipid(1.11%)and polyunsaturated fatty acids(123.01mg/g),suggesting potential for bioactive lipid extraction in nutraceutical applications.By contrast,basal fronds were enriched in saturated(86.41 mg/g)and monounsaturated fatty acids(38.38 mg/g).The essential amino acid index ranged from 0.74 to 0.87,and total amino acid content peaked in middle fronds(77.90 mg/g),highlighting their high protein quality.Apical fronds had the highest calcium content(15611.95 mg/kg),and receptacles contained the highest phosphorus content(1224.45 mg/kg),supporting targeted mineral supplementation.The maximum concentrations of all trace elements were found in receptacles,reinforcing their pharmaceutical potential.This study provides foundational data on the nutrient composition of different thallus parts of S.muticum,which may support future research and potential utilization efforts in China.展开更多
Polyphenol is a kind of natural active substance with immunoregulatory activity.In this study,the immunoregulatory activities of Sargassum carpophyllum polyphenol(SCP)on the allergic reactions induced by shrimp tropom...Polyphenol is a kind of natural active substance with immunoregulatory activity.In this study,the immunoregulatory activities of Sargassum carpophyllum polyphenol(SCP)on the allergic reactions induced by shrimp tropomyosin were investigated.As the results,the SCP performed high hyaluronidase inhibition ability,which even greater than sodium chromoglycate(an anti-allergic medicine).SCP was able to affect the allergic reactions of RAW 264.7 macrophage cells via regulating the nuclear factor kappa-B(NF-κB),c-Jun N-terminal kinase 1/2(JNK1/2),extracellular regulated protein kinase 1/2(Erk1/2)and p38 mitogen-activated protein kinase(MAPK)cellular signaling pathways,weakened the degranulation degree and secretion of interleukin-4(IL-4),tumor necrosis factor-α(TNF-α)and histamine,and promoted the secretion of interleukin-10(IL-10)from RBL-2H3 mast cells.In BALB/c mouse model assay,after gavage of SCP,the mouse allergic symptoms got significantly alleviated,the cytokine(IL-4,IL-10 and interferon-γ(IFN-γ))secretion of mouse spleen lymphocytes were significantly declined and the allergic lung damage was relieved,which indicated SCP performed strong both in vitro and in vivo anti-allergic functions.Therefore,SCP could serve as candidate anti-allergic bioactive substances for shrimp induced allergy via immunoregulation.展开更多
High electrochemical performance supercapacitors require activated carbon with high specific surface area,suitable pore size distribution and surface properties,and high electrical conductivity as electrode materials,...High electrochemical performance supercapacitors require activated carbon with high specific surface area,suitable pore size distribution and surface properties,and high electrical conductivity as electrode materials,whereas there exists a trade-off relationship between specific surface area and electrical conductivity,which is not well met by a single type of carbon source.To solve this problem,the coal and sargassum are adopted to obtain the coupling product via co-thermal dissolution,followed by carbonization and KOH activation.The effects of mixing mass ratio and activation temperature on the prepared activated carbon(AC)are investigated using single factor experimental method.The experimental results show that AC_(1/3-800)has abundant micropore and mesopore content,good pore structure connectivity,high electrical conductivity and good wettability,and superior electrochemical properties compared with other activated carbons prepared in this experiment.Its total specific surface area is up to 2098.5 m^(2)·g^(-1),the pore volume is up to 1.33 cm^(3)·g^(-1),the content of mespores with diameter of 6-8 nm is significantly increased,and the pore size distribution is wide and uniform.When the current density increases from 0.1 to 10 A·g^(-1),the gravimetric capacitance decreases from 219 to 186 F·g^(-1)with a capacitance retention of 84.9%,the equivalent series resistance is very small,and the rate performance and reversibility of charging and discharging have also been excellent.展开更多
Tissue culture is one of the most promising and practical methods for conserving endangered plant species.Therefore,the present study evaluates the conservation of the endangered seaweed Sargassum fusiforme through ti...Tissue culture is one of the most promising and practical methods for conserving endangered plant species.Therefore,the present study evaluates the conservation of the endangered seaweed Sargassum fusiforme through tissue culturing techniques from different explants for the first time.Besides,the genetic variation of the mother plant and cultivated explants using inter-simple sequence repeat(ISSR)techniques;this methodology was the first to be illustrated in such work for algae tissue culturing.The regeneration results have shown that different explants could induce shoot and rhizoid morphogenesis with a total number of blades of 2436,1011,1466,678,and 6 from apical parts,stipe with blades,two-segmented seedlings,rhizoids,and stipe without blades,respectively.The total length was 234,181.8,83.5,and 81.8 cm from the two-segmented seedlings,apical parts,stipe without blades,and stipe with blades,respectively.At the same time,the total wet weight was 73.148,48.369,35.731,18.588,and 2.035 g from the apical parts,the two-segmented seedlings,stipe with blades,rhizoids,and stipe without blades,respectively.Micropropagation of S.fusiforme was successfully achieved with apical,stipe,and stolon segments using free PES media.It is suggested that the applied genetic fingerprint is valid for S.fusiforme and will respond well to molecular marker assistance in cultivation.The significance of S.fusiforme and its exposure to being endangered due to over-exploitation have made its regeneration in vitro a subject of interest in this study.Thus,this report represents the successful regeneration of S.fusiforme and explores the genetic uniformity or somaclonal variation of the obtained seedlings using the ISSR-PCR marker for the first time.展开更多
Species of Sargassum are distributed worldwide, and are of great ecological and economic importance in marine ecosystems and bioresources. In this study, transcriptome sequencings of six Sargassum species were perform...Species of Sargassum are distributed worldwide, and are of great ecological and economic importance in marine ecosystems and bioresources. In this study, transcriptome sequencings of six Sargassum species were performed for the first time using an Illumina platform. For each sample, a total of 2.1-2.5 Gb of nucle-otides are collected and assembled into 69 871-116 790 scaffolds, with an average length of 410-550 bp and N50 length of 756-1 462 bp. A total of 20 512-28 684 unigenes of each sample were annotated and compared well with known gene sequences from nr database. Clusters of Orthologous Groups （COG）, gene ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） analyses were also performed for further un-derstanding of gene functions and regulation pathways. Gene expression levels were calculated based on RPKM values and compared among these species, especially for those genes related to carbohydrate metab-olism. Cluster analyses indicated that the differences of global gene expression between S. fusiforme, which was nominated as Hizikia fusiformis before, and other five species were not significant. Further phylogenet-ic analysis of 108 orthologous genes confirmed that S. fusiforme had closer relationship with S. hemiphyllum rather than S. horneri. These transcriptome data provided valuable information for better understanding of genome and gene characteristics of Sargassum algae and benefiting comparative and phylogenetic studies of Phaeophyceae species in future studies.展开更多
The abnormal increase of drifting brown alga Sargassum horneri was initially documented in 2007.It formed blooms along the coast of East China Sea and Yellow Sea in 2017.In this study,we investigated the changes of sp...The abnormal increase of drifting brown alga Sargassum horneri was initially documented in 2007.It formed blooms along the coast of East China Sea and Yellow Sea in 2017.In this study,we investigated the changes of specific growth rate and resource accumulation of drifting S.horneri in response to temperature and nitrogen richness at different growth stages under laboratory condition.The investigation lasted from June 2015 to April 2016 with the observation made every two months.The results showed that the life cycle consists of a few growth stages dividable with morphological characteristics.The growth can be divided into shedding and withering(August),rapid growing(October to September),slow growing(February),rapid growing(April)and maturation(June)stages.Under the experimental condition,algal segments were found to grow at temperatures ranging from 5 to 25℃in 12 days even when nitrogen is deficient.A significant difference in the special growth rate(SGR)between nitrogen-enriched and nitrogen-removed treatments was found in most months(P<0.05).SGR was lower in August and February than that in other months.Nitrogen and chlorophyll contents in algal segments were different among different temperatures,nitrogen supply and seasons.Nitrogen content was higher in February and April than that in other months in both nitrogen-enriched and nitrogen-removed treatments.The results showed that the demand of S.horneri for nitrogen increased in spring when it grows fast.It is likely that the high temperature and nitrogen concentration in winter and spring lead to the high biomass accumulation of drifting S.horneri.展开更多
基金financially supported by the National Key Research and Development Program of China(No.2024YFD2400300)the National Natural Science Foundation of China(No.32402995)+1 种基金the Taishan Scholar Foundation of Shandong Province(No.tsqn202211067)the Fundamental Research Fund for the Central Universities(No.202262002)。
文摘Sargassum muticum is a globally distributed seaweed with high economic potential,yet its detailed nutritional composition remains undercharacterized.In this study,we analyzed the nutritional profiles of five thallus portions,namely apical fronds,middle fronds,basal fronds,receptacles and air bladders,to assess their economic value accordingly.Results showed that middle fronds had the highest crude protein(31.98%)and crude fiber(7.10%)contents,indicating their suitability for protein-rich functional foods or dietary fiber supplements.Receptacles exhibited the highest levels of crude lipid(1.11%)and polyunsaturated fatty acids(123.01mg/g),suggesting potential for bioactive lipid extraction in nutraceutical applications.By contrast,basal fronds were enriched in saturated(86.41 mg/g)and monounsaturated fatty acids(38.38 mg/g).The essential amino acid index ranged from 0.74 to 0.87,and total amino acid content peaked in middle fronds(77.90 mg/g),highlighting their high protein quality.Apical fronds had the highest calcium content(15611.95 mg/kg),and receptacles contained the highest phosphorus content(1224.45 mg/kg),supporting targeted mineral supplementation.The maximum concentrations of all trace elements were found in receptacles,reinforcing their pharmaceutical potential.This study provides foundational data on the nutrient composition of different thallus parts of S.muticum,which may support future research and potential utilization efforts in China.
基金financial support from National Natural Science Foundation of China(32102091)Fujian Province Specialized Project of Promoting High-Quality Development of Marine and Fishery Industry(FJHYF-L-2023-26)+4 种基金Shandong Provincial Natural Science Foundation(ZR2021QC086)China Postdoctoral Science Foundation(2021M693026)Postdoctoral Innovation Project of Shandong Province(862105033022)Qingdao Postdoctoral Applied Research Project(862105040045)Research Funding of Ocean University of China(862001013187).
文摘Polyphenol is a kind of natural active substance with immunoregulatory activity.In this study,the immunoregulatory activities of Sargassum carpophyllum polyphenol(SCP)on the allergic reactions induced by shrimp tropomyosin were investigated.As the results,the SCP performed high hyaluronidase inhibition ability,which even greater than sodium chromoglycate(an anti-allergic medicine).SCP was able to affect the allergic reactions of RAW 264.7 macrophage cells via regulating the nuclear factor kappa-B(NF-κB),c-Jun N-terminal kinase 1/2(JNK1/2),extracellular regulated protein kinase 1/2(Erk1/2)and p38 mitogen-activated protein kinase(MAPK)cellular signaling pathways,weakened the degranulation degree and secretion of interleukin-4(IL-4),tumor necrosis factor-α(TNF-α)and histamine,and promoted the secretion of interleukin-10(IL-10)from RBL-2H3 mast cells.In BALB/c mouse model assay,after gavage of SCP,the mouse allergic symptoms got significantly alleviated,the cytokine(IL-4,IL-10 and interferon-γ(IFN-γ))secretion of mouse spleen lymphocytes were significantly declined and the allergic lung damage was relieved,which indicated SCP performed strong both in vitro and in vivo anti-allergic functions.Therefore,SCP could serve as candidate anti-allergic bioactive substances for shrimp induced allergy via immunoregulation.
基金supported by the Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China(2023KJ122)State Grid Shandong Electric Power Company Science and Technology Project(520618240009)Doctoral Research Fund of Shandong Jianzhu University(XNBS1838).
文摘High electrochemical performance supercapacitors require activated carbon with high specific surface area,suitable pore size distribution and surface properties,and high electrical conductivity as electrode materials,whereas there exists a trade-off relationship between specific surface area and electrical conductivity,which is not well met by a single type of carbon source.To solve this problem,the coal and sargassum are adopted to obtain the coupling product via co-thermal dissolution,followed by carbonization and KOH activation.The effects of mixing mass ratio and activation temperature on the prepared activated carbon(AC)are investigated using single factor experimental method.The experimental results show that AC_(1/3-800)has abundant micropore and mesopore content,good pore structure connectivity,high electrical conductivity and good wettability,and superior electrochemical properties compared with other activated carbons prepared in this experiment.Its total specific surface area is up to 2098.5 m^(2)·g^(-1),the pore volume is up to 1.33 cm^(3)·g^(-1),the content of mespores with diameter of 6-8 nm is significantly increased,and the pore size distribution is wide and uniform.When the current density increases from 0.1 to 10 A·g^(-1),the gravimetric capacitance decreases from 219 to 186 F·g^(-1)with a capacitance retention of 84.9%,the equivalent series resistance is very small,and the rate performance and reversibility of charging and discharging have also been excellent.
基金supported by the grants from China Asia-Pacific Economic Cooperation(Nos.HV180101 and HV01-190101)the Xiamen Science and Technology Program(No.3502Z20161162)the Specific Fund Project of Xiamen Marine and Fishery Development(No.19CZP011HJ08).
文摘Tissue culture is one of the most promising and practical methods for conserving endangered plant species.Therefore,the present study evaluates the conservation of the endangered seaweed Sargassum fusiforme through tissue culturing techniques from different explants for the first time.Besides,the genetic variation of the mother plant and cultivated explants using inter-simple sequence repeat(ISSR)techniques;this methodology was the first to be illustrated in such work for algae tissue culturing.The regeneration results have shown that different explants could induce shoot and rhizoid morphogenesis with a total number of blades of 2436,1011,1466,678,and 6 from apical parts,stipe with blades,two-segmented seedlings,rhizoids,and stipe without blades,respectively.The total length was 234,181.8,83.5,and 81.8 cm from the two-segmented seedlings,apical parts,stipe without blades,and stipe with blades,respectively.At the same time,the total wet weight was 73.148,48.369,35.731,18.588,and 2.035 g from the apical parts,the two-segmented seedlings,stipe with blades,rhizoids,and stipe without blades,respectively.Micropropagation of S.fusiforme was successfully achieved with apical,stipe,and stolon segments using free PES media.It is suggested that the applied genetic fingerprint is valid for S.fusiforme and will respond well to molecular marker assistance in cultivation.The significance of S.fusiforme and its exposure to being endangered due to over-exploitation have made its regeneration in vitro a subject of interest in this study.Thus,this report represents the successful regeneration of S.fusiforme and explores the genetic uniformity or somaclonal variation of the obtained seedlings using the ISSR-PCR marker for the first time.
基金The National Natural Science Foundation of China under contract Nos 31140070,31271397 and 41206116the algal transcrip-tome sequencing was supported by 1KP Project(www.onekp.com)
文摘Species of Sargassum are distributed worldwide, and are of great ecological and economic importance in marine ecosystems and bioresources. In this study, transcriptome sequencings of six Sargassum species were performed for the first time using an Illumina platform. For each sample, a total of 2.1-2.5 Gb of nucle-otides are collected and assembled into 69 871-116 790 scaffolds, with an average length of 410-550 bp and N50 length of 756-1 462 bp. A total of 20 512-28 684 unigenes of each sample were annotated and compared well with known gene sequences from nr database. Clusters of Orthologous Groups （COG）, gene ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） analyses were also performed for further un-derstanding of gene functions and regulation pathways. Gene expression levels were calculated based on RPKM values and compared among these species, especially for those genes related to carbohydrate metab-olism. Cluster analyses indicated that the differences of global gene expression between S. fusiforme, which was nominated as Hizikia fusiformis before, and other five species were not significant. Further phylogenet-ic analysis of 108 orthologous genes confirmed that S. fusiforme had closer relationship with S. hemiphyllum rather than S. horneri. These transcriptome data provided valuable information for better understanding of genome and gene characteristics of Sargassum algae and benefiting comparative and phylogenetic studies of Phaeophyceae species in future studies.
基金supported by the Public Science and Technology Research Funds of Ocean from State Oceanic Administration, People’s Republic of China (Nos. 200905 0202, 201405040-4)
文摘The abnormal increase of drifting brown alga Sargassum horneri was initially documented in 2007.It formed blooms along the coast of East China Sea and Yellow Sea in 2017.In this study,we investigated the changes of specific growth rate and resource accumulation of drifting S.horneri in response to temperature and nitrogen richness at different growth stages under laboratory condition.The investigation lasted from June 2015 to April 2016 with the observation made every two months.The results showed that the life cycle consists of a few growth stages dividable with morphological characteristics.The growth can be divided into shedding and withering(August),rapid growing(October to September),slow growing(February),rapid growing(April)and maturation(June)stages.Under the experimental condition,algal segments were found to grow at temperatures ranging from 5 to 25℃in 12 days even when nitrogen is deficient.A significant difference in the special growth rate(SGR)between nitrogen-enriched and nitrogen-removed treatments was found in most months(P<0.05).SGR was lower in August and February than that in other months.Nitrogen and chlorophyll contents in algal segments were different among different temperatures,nitrogen supply and seasons.Nitrogen content was higher in February and April than that in other months in both nitrogen-enriched and nitrogen-removed treatments.The results showed that the demand of S.horneri for nitrogen increased in spring when it grows fast.It is likely that the high temperature and nitrogen concentration in winter and spring lead to the high biomass accumulation of drifting S.horneri.
