This study provides the first comprehensive analysis of antibiotic resistance&genomic characterization of Staphylococ-cus saprophyticus isolated from Southern Ocean.Antibiotic susceptibility profiling of S.saproph...This study provides the first comprehensive analysis of antibiotic resistance&genomic characterization of Staphylococ-cus saprophyticus isolated from Southern Ocean.Antibiotic susceptibility profiling of S.saprophyticus revealed complete resistance to Cefixime,Norfloxacin,Azithromycin,and Metronidazole,while susceptibility was observed for Ampicil-lin,Doxycycline,Tetracycline,Ciprofloxacin,and Co-trimoxazole.Whole-genome sequencing and comparative genomics analysis with 21 closely related strains identified antimicrobial resistance(AMR)genes viz a viz vanY(in the vanM cluster),sdrM,sepA,norC,salE,fusD,and fosBx1.Among these,vanY exhibited the highest prevalence,followed by sdrM and sepA.Study also showed varying AMR gene distributions,with some strains harboring all seven resistance genes.The presence of antibiotic-resistant S.saprophyticus in the Southern Ocean highlights the potential anthropogenic influence on microbial communities leading to AMR among native microbial communities and highlights the urgent need for further studies on AMR in remote marine environments and its mitigation strategies.The study enhances understanding of the global dis-semination of AMR by investigating S.saprophyticus in one of the pristine and isolated ecosystems on Earth.Our findings demonstrates that even remote environments are not immune to the spread of AMR.Furthermore,the study provides crucial insights into resistance mechanisms and the identification of resistance genes in a non-clinical,extreme environment puts light on microbial adaptability,and ecological resilience in response to environmental stressors.展开更多
Objectives:As considered highly resistant to antibiotics like mecillinam,the rise of Staphylococcus saprophyticus(S.saprophyticus)contamination of fresh foods and food processing environments necessitates the developm...Objectives:As considered highly resistant to antibiotics like mecillinam,the rise of Staphylococcus saprophyticus(S.saprophyticus)contamination of fresh foods and food processing environments necessitates the development of a new antimicrobial approach for food safety control.This study aimed to investigate the synergistic effect of food-grade curcumin(CUR)and blue light-emitting diode(LED)on S.saprophyticus.Materials and Methods:S.saprophyt/ciys was subjected to the synergistic treatment at 4℃ and 25℃.The influence of parameters,including CUR concentration,light dose,and pH incubation time on the inactivation of S.saprophyticus vjqs characterized through plate counting method.Results:The combined treatment of CUR and blue light irradiation significantly(P<0.05)reduced bacterial counts and the antimicrobial effect was in a CUR concentration and light dose-dependent manner.Moreover,refrigeration temperature(4℃)significantly(P<0.05)enhanced the antibacterial effect at neutral pH condition(6.2-72),resulting in approximately 6.0 log reductions.Under acidic condition(pH 2.2-5.2),there was no significant difference in bacterial population reduction between treatments at both temperatures.Conclusions:These findings proposed that synergistic interaction of CUR and 460 nm LED under refrigerated temperature could enhance the inactivation of S.saprophyticus at neutral pH condition.展开更多
基金conducted with the financial assistance of the Ministry of Earth Sciences(MOES),Govt.of India(MOES/REACHOUT/CNA/2022).
文摘This study provides the first comprehensive analysis of antibiotic resistance&genomic characterization of Staphylococ-cus saprophyticus isolated from Southern Ocean.Antibiotic susceptibility profiling of S.saprophyticus revealed complete resistance to Cefixime,Norfloxacin,Azithromycin,and Metronidazole,while susceptibility was observed for Ampicil-lin,Doxycycline,Tetracycline,Ciprofloxacin,and Co-trimoxazole.Whole-genome sequencing and comparative genomics analysis with 21 closely related strains identified antimicrobial resistance(AMR)genes viz a viz vanY(in the vanM cluster),sdrM,sepA,norC,salE,fusD,and fosBx1.Among these,vanY exhibited the highest prevalence,followed by sdrM and sepA.Study also showed varying AMR gene distributions,with some strains harboring all seven resistance genes.The presence of antibiotic-resistant S.saprophyticus in the Southern Ocean highlights the potential anthropogenic influence on microbial communities leading to AMR among native microbial communities and highlights the urgent need for further studies on AMR in remote marine environments and its mitigation strategies.The study enhances understanding of the global dis-semination of AMR by investigating S.saprophyticus in one of the pristine and isolated ecosystems on Earth.Our findings demonstrates that even remote environments are not immune to the spread of AMR.Furthermore,the study provides crucial insights into resistance mechanisms and the identification of resistance genes in a non-clinical,extreme environment puts light on microbial adaptability,and ecological resilience in response to environmental stressors.
基金funded by the National Natural Science Foundation of China(No.31701640)the Beijing Advanced Innovation Center for Food Nutrition and Human Health,China.
文摘Objectives:As considered highly resistant to antibiotics like mecillinam,the rise of Staphylococcus saprophyticus(S.saprophyticus)contamination of fresh foods and food processing environments necessitates the development of a new antimicrobial approach for food safety control.This study aimed to investigate the synergistic effect of food-grade curcumin(CUR)and blue light-emitting diode(LED)on S.saprophyticus.Materials and Methods:S.saprophyt/ciys was subjected to the synergistic treatment at 4℃ and 25℃.The influence of parameters,including CUR concentration,light dose,and pH incubation time on the inactivation of S.saprophyticus vjqs characterized through plate counting method.Results:The combined treatment of CUR and blue light irradiation significantly(P<0.05)reduced bacterial counts and the antimicrobial effect was in a CUR concentration and light dose-dependent manner.Moreover,refrigeration temperature(4℃)significantly(P<0.05)enhanced the antibacterial effect at neutral pH condition(6.2-72),resulting in approximately 6.0 log reductions.Under acidic condition(pH 2.2-5.2),there was no significant difference in bacterial population reduction between treatments at both temperatures.Conclusions:These findings proposed that synergistic interaction of CUR and 460 nm LED under refrigerated temperature could enhance the inactivation of S.saprophyticus at neutral pH condition.
