Objective:To report the first imported case of cerebral schistosomiasis mansoni in China and highlight the public health risks posed by this disease.Methods:We conducted an epidemiological investigation,performed labo...Objective:To report the first imported case of cerebral schistosomiasis mansoni in China and highlight the public health risks posed by this disease.Methods:We conducted an epidemiological investigation,performed laboratory testing of clinical samples,and collected diagnostic and treatment data.The infection source was determined through comprehensive analysis of epidemiological history,clinical manifestations,and laboratory findings.Results:Before returning to China,the patient had resided for nearly 10 years in schistosomiasis-endemic regions,including Angola and the Democratic Republic of the Congo(DRC),where mountain spring water was used for drinking and daily activities.One month before returning to China,the patient developed central nervous system symptoms,including limb weakness and slowed reactions.Laboratory testing revealed markedly elevated eosinophil(EOS)levels,with both percentage(25.0%)and absolute count(3.30×109/L)exceeding normal ranges.Highthroughput sequencing(HTS)of blood samples identified Schistosoma mansoni DNA sequences,and microscopic stool examination detected S.mansoni eggs.Conclusion:China is not an endemic area for S.mansoni.This study reports the first imported case of cerebral schistosomiasis mansoni in China.With the continuous emergence of imported S.mansoni cases and the gradual expansion of intermediate host breeding grounds,we should actively monitor the potential risk of local transmission occurring in China.Enhanced protective awareness among outbound tourists and strengthened public health surveillance are essential measures to counter the threat posed by imported cases.展开更多
Pituitary neuroendocrine tumors(PitNETs)are pathologically characterized by dysregulation of neuroendocrine function and systemic disruption of hormonal homeostasis,yet their regulatory effects on peripheral immune ne...Pituitary neuroendocrine tumors(PitNETs)are pathologically characterized by dysregulation of neuroendocrine function and systemic disruption of hormonal homeostasis,yet their regulatory effects on peripheral immune networks remain poorly characterized.Here,we systematically analyzed bulk RNA sequencing(RNA‑seq)from 883 PitNET tumors,108 PitNET‑associated peripheral blood mononuclear cells(PBMC)samples,and 175 healthy PBMC controls,combined with 69 single‑cell RNA sequencing(scRNA-seq)samples covering tumors,normal pituitaries,as well as tumor‑derived and normal PBMCs.We identified a systemic immune disequilibrium in PitNET patients,characterized by increased circulating lymphocyte proportions,accompanied by upregulated cytokine-receptor interaction signatures.Notably,tumor resection reversed this imbalance,as supported by the normalization of monocyte and neutrophil counts,validated by flow cytometry and routine blood data from 600 samples(200 healthy controls and 200 PitNET patients with paired pre-and post-surgery follow‑up).Trajectory analysis identified terminally differentiated,secretory-specialized cell populations with lineage-specific hormone and cytokine hypersecretion.Ligand-receptor inference suggested these tumor-derived factors potentially engage circulating immune cell receptors.A random‑forest classifier based on PBMC transcriptomes distinguished PitNET subtypes,underscoring the diagnostic potential of peripheral immune signatures.Furthermore,in an estrogen-induced rat model,elevated PRL level coincided with the same peripheral immune skewing.Overall,our work provides a valuable resource and demonstrates PitNETs can be systemic immune modulators,where intrinsic hormone secretory activity and monocyte-lymphocyte imbalance collectively drive peripheral immune dysfunction.展开更多
文摘Objective:To report the first imported case of cerebral schistosomiasis mansoni in China and highlight the public health risks posed by this disease.Methods:We conducted an epidemiological investigation,performed laboratory testing of clinical samples,and collected diagnostic and treatment data.The infection source was determined through comprehensive analysis of epidemiological history,clinical manifestations,and laboratory findings.Results:Before returning to China,the patient had resided for nearly 10 years in schistosomiasis-endemic regions,including Angola and the Democratic Republic of the Congo(DRC),where mountain spring water was used for drinking and daily activities.One month before returning to China,the patient developed central nervous system symptoms,including limb weakness and slowed reactions.Laboratory testing revealed markedly elevated eosinophil(EOS)levels,with both percentage(25.0%)and absolute count(3.30×109/L)exceeding normal ranges.Highthroughput sequencing(HTS)of blood samples identified Schistosoma mansoni DNA sequences,and microscopic stool examination detected S.mansoni eggs.Conclusion:China is not an endemic area for S.mansoni.This study reports the first imported case of cerebral schistosomiasis mansoni in China.With the continuous emergence of imported S.mansoni cases and the gradual expansion of intermediate host breeding grounds,we should actively monitor the potential risk of local transmission occurring in China.Enhanced protective awareness among outbound tourists and strengthened public health surveillance are essential measures to counter the threat posed by imported cases.
基金supported by the National Research Center for Translational Medicine under grant number NRCTM(SH)2023-15(to Z-B.W.)Fundamental Research Funds for the Central Universities(No.YG2023ZD06 to Z-B.W.)+3 种基金National Natural Science Foundation of China(82472640 to Z-B.W.,82373131 and 82573142 to S-J.L.,82200153 to Y-T.D.,82500265 to S-S.Y.)Health care leader of Shanghai Municipal Health Commission(No.2022LJ006 to Z-B.W.)the Natural Science Foundation of Shanghai,China(25ZR1402349 to S-S.Y.)the Innovative Research Team of HighLevel Local Universities in Shanghai.
文摘Pituitary neuroendocrine tumors(PitNETs)are pathologically characterized by dysregulation of neuroendocrine function and systemic disruption of hormonal homeostasis,yet their regulatory effects on peripheral immune networks remain poorly characterized.Here,we systematically analyzed bulk RNA sequencing(RNA‑seq)from 883 PitNET tumors,108 PitNET‑associated peripheral blood mononuclear cells(PBMC)samples,and 175 healthy PBMC controls,combined with 69 single‑cell RNA sequencing(scRNA-seq)samples covering tumors,normal pituitaries,as well as tumor‑derived and normal PBMCs.We identified a systemic immune disequilibrium in PitNET patients,characterized by increased circulating lymphocyte proportions,accompanied by upregulated cytokine-receptor interaction signatures.Notably,tumor resection reversed this imbalance,as supported by the normalization of monocyte and neutrophil counts,validated by flow cytometry and routine blood data from 600 samples(200 healthy controls and 200 PitNET patients with paired pre-and post-surgery follow‑up).Trajectory analysis identified terminally differentiated,secretory-specialized cell populations with lineage-specific hormone and cytokine hypersecretion.Ligand-receptor inference suggested these tumor-derived factors potentially engage circulating immune cell receptors.A random‑forest classifier based on PBMC transcriptomes distinguished PitNET subtypes,underscoring the diagnostic potential of peripheral immune signatures.Furthermore,in an estrogen-induced rat model,elevated PRL level coincided with the same peripheral immune skewing.Overall,our work provides a valuable resource and demonstrates PitNETs can be systemic immune modulators,where intrinsic hormone secretory activity and monocyte-lymphocyte imbalance collectively drive peripheral immune dysfunction.
