Spartina alterniflora invasions seriously threaten the structure and functions of coastal wetlands in China.In this study,the Suaeda salsa community in the Yellow River Estuary wetland was monitored using long-term La...Spartina alterniflora invasions seriously threaten the structure and functions of coastal wetlands in China.In this study,the Suaeda salsa community in the Yellow River Estuary wetland was monitored using long-term Landsat satellite images acquired from 1997 to 2020 to quantify the impact of changes in hydrological connectivity induced by S.alterniflora on neighboring vegetation com-munities.The results showed that S.alterniflora rapidly expanded in the estuary area at a rate of 4.91 km^(2)/yr from 2010 to 2020.At the same time,the hydrological connectivity of the area and the distribution of S.salsa changed significantly.Small tidal creeks dominated the S.alterniflora landscape.The number of tidal creeks increased significantly,but their average length decreased and they tended to develop in a horizontal tree-like pattern.Affected by the changes in hydrological connectivity due to the S.alterniflora invasion,the area of S.salsa decreased by 41.1%,and the degree of landscape fragmentation increased from 1997 to 2020.Variations in the Largest Patch Index(LPI)indicated that the S.alterniflora landscape had become the dominant landscape type in the Yellow River Estuary.The res-ults of standard deviation ellipse(SDE)and Pearson’s correlation analyses indicated that a well-developed hydrological connectivity could promote the maintenance of the S.salsa landscape.The degradation of most S.salsa communities is caused by the influence of S.alterniflora on the morphological characteristics of the hydrological connectivity of tidal creek systems.展开更多
[Objective] Under salt stress condition,effects of inoculation of arbuscular mycorrhizal fungi Glomus mosseae on SOD and CAT activity in Suaeda salsa seedlings under salt stress were studied.[Method]There were 2 NaCl ...[Objective] Under salt stress condition,effects of inoculation of arbuscular mycorrhizal fungi Glomus mosseae on SOD and CAT activity in Suaeda salsa seedlings under salt stress were studied.[Method]There were 2 NaCl levels,namely 0 and 400 mmol/L and each NaCl contained 2 treatments,one is inoculated by Glomus mosseae and the other is control.The growths of Suaeda salsa,SOD and CAT activities as well as MDA content in leaves was determined.[Result]Under salt stress condition,Glomus mosseae could increase the growths of Suaeda salsa,SOD and CAT activities in leaves and decreased MDA content in leaves.[Conclusion]It preliminarily demonstrated that Arbuscular Mycorrhizal(AM)Fungi could increase salt resistance of Suaeda salsa by increasing the activities of SOD and CAT as well as alleviating membrane injury.展开更多
AdoMet plays numerous roles of being the major methyl-group donor in trans-methylation reactions. To gain insight into the possible functions of the AdoMet protein of Suaeda salsa L. in response to salt stress, S aden...AdoMet plays numerous roles of being the major methyl-group donor in trans-methylation reactions. To gain insight into the possible functions of the AdoMet protein of Suaeda salsa L. in response to salt stress, S adenosylmethionine synthetase gene (SAMS2) was analyzed. We isolated SAMS2 cDNA clone (AF321001) from a lambda -Zap cDNA library constructed from the halophyte S. salsa Pall aerial tissue treated with 400 mmol/L NaCl. SsSAMS2 was found to encode a S-adenolyl-L-methionine synthetase enzyme (AdoMet synthetase). The fragment was 1 531 bp with an open reading frame of 395 amino acids, the calculated molecular weight was about 43 kD. SsSAMS2 showed the highest homology to SAMS2 gene of Catharanthus roseus G. Don., with 93% identity in deduced amino acid sequence. Southern blotting analysis showed that SsSAMS2 might be a two-copy gene in S. salsa genome. Northern blot indicated that the cDNA was up-regulated by salt and other stresses. Enzyme activity assay indicated that the activity of SAMS2 increased under NaCl stress.展开更多
Two different cDNA clones (Sscat1 and Sscat2) encoding catalase, the primary important H2O2-scavenging enzyme, were isolated from a AZap-cDNA library constructed from a 400 mmol/L NaCl-treated library of Suaeda salsa ...Two different cDNA clones (Sscat1 and Sscat2) encoding catalase, the primary important H2O2-scavenging enzyme, were isolated from a AZap-cDNA library constructed from a 400 mmol/L NaCl-treated library of Suaeda salsa ( L.) Pall aerial tissue. Sscat1 (1.7 kb) contains a full open reading frame of 492 amino acids and Sscat2 (1.1 kb) is a partial clone. BLAST analysis indicates that the two clones share 71.9% identity in nucleotide sequence and 75% identity in deduced amino acid sequence within the last 287 amino acid residues of Sscat1. Southern blotting analysis showed that Sscat1 is multicopy in S. salsa genome, while Sscat2 is a single copy gene. Northern blotting analysis showed a rapid increase in the steady-level of both genes in roots after 48 It salt treatment, but only Sscat1 was induced in salinity treated leaves. Time-course analysis carried out in leaves confirmed that Sscat1 was induced by salt stress, in contrast to Sscat2. These implied that the expression of Sscat1 and Sscat2 genes are differentially regulated in S. salsa. The activity of total catalase is dramatically increased in response to salt stress.展开更多
Suaeda salsa L. seedlings grown in Hoagland nutrient solution were treated with different concentrations of NaCl combined with two levels of K + (12 μmol/L and 6 mmol/L) to study the K + nutrition effect on plant g...Suaeda salsa L. seedlings grown in Hoagland nutrient solution were treated with different concentrations of NaCl combined with two levels of K + (12 μmol/L and 6 mmol/L) to study the K + nutrition effect on plant growth and leaf tonoplast V-H +-ATPase and V-H +-PPase activity. Increase of K + supply in the culture solution markedly increased the fresh weight, dry weight and K + content of S. salsa plants. Western blot analysis showed that the leaf V-H +-ATPase of S. salsa was at least composed of A,B,C,D,E and c subunits, and their expression decreased with the increase of NaCl concentration under K + starvation (12 μmol/L K +), but increased under normal K + application (6 mmol/L K +). Leaf V-H +-PPase molecular weight was about 72.6 kD and its expression increased as NaCl concentration increased under both high or low levels of K + concentration in nutrient solution. There was a positive correlation between of V-H +-ATPase or V-H +-PPase activity and the amounts of their expression. Results in this study suggest that K + nutrition plays an important role in the salt tolerance of S. salsa, and K + is involved in the regulation of V-H +-ATPase or V-H +-PPase activity under salt stress.展开更多
Introduction The plants of genus Cistanche G. Beck, which belong to Orobanchaceae family, include about twenty species, and there are four species and one variety are distributed in the northwest part of China. The d...Introduction The plants of genus Cistanche G. Beck, which belong to Orobanchaceae family, include about twenty species, and there are four species and one variety are distributed in the northwest part of China. The dried fleshy stem of Cistanche genus plant known as ＇Roucongrong＇ in Chinese traditional medicine, has long been used for kidney deficiency, female infertility, morbid leucorrhea, and neurasthenia. Studies on active components and pharmacological activities show that it possesses significant activities in enhancing potency, anti-fatigue, immuno-modulability, etc, and phenylethanoid glycosides are the major active components. Due to excessive exploitation, its natural resources are facing with exhaustion. In order to improve this situation, the plant has been cultivated on a large scale in the northwest part of China. Up to now, the chemical investigations of the cultivated Cistartcbe salsa have not been undertaken. In this paper we report seven phenylethanoid glycosides from the cultivated Cistanche salsa： echinacoside （1）, cistanoside A （2）, acteoside （3）, isoacteoside （4）, 2＇-acetylacteoside （5）, tubuloside B （6）, and eutigoside A （7）. Among them, compound 7 isolated fronl family Orobanchaceae was reported previously, and compounds 1, 2, 3, 4, 5 and 6 from the cultivated Cistanche salsa are reported for the first time.展开更多
[Objective] This study was to investigate the Suaeda salsa community characteristics,further getting the soil chemical properties.[Method] The paired-data of field spectra and corresponding soil physical-chemical prop...[Objective] This study was to investigate the Suaeda salsa community characteristics,further getting the soil chemical properties.[Method] The paired-data of field spectra and corresponding soil physical-chemical property of seventeen samples was used to reveal the relationship between soil chemical property and field spectra（visible and near infra-red spectra）of S.salsa.[Result] The second derivative spectrum of S.salsa at 1 121 nm could reflect the changes of soil organic matter and soil total nitrogen,and that at 1 208 nm could commendably indicate changes of soil total phosphorus and at 724 nm could indicate changes of soil pH.The first derivative spectrum of S.salsa at 353 nm can indicate changes of soil available potassium,and that at 950 nm could commendably reflect the changes of soil salt content.[Conclusion] Our results laid basis for monitoring chemical property of soil covered with S.salsa using remote sensing technology.展开更多
基金Under the auspices of Key Program of the National Natural Science Foundation of China(No.U2006215,U1806218)the National Key R&D Program of China(No.2017YFC0505902)。
文摘Spartina alterniflora invasions seriously threaten the structure and functions of coastal wetlands in China.In this study,the Suaeda salsa community in the Yellow River Estuary wetland was monitored using long-term Landsat satellite images acquired from 1997 to 2020 to quantify the impact of changes in hydrological connectivity induced by S.alterniflora on neighboring vegetation com-munities.The results showed that S.alterniflora rapidly expanded in the estuary area at a rate of 4.91 km^(2)/yr from 2010 to 2020.At the same time,the hydrological connectivity of the area and the distribution of S.salsa changed significantly.Small tidal creeks dominated the S.alterniflora landscape.The number of tidal creeks increased significantly,but their average length decreased and they tended to develop in a horizontal tree-like pattern.Affected by the changes in hydrological connectivity due to the S.alterniflora invasion,the area of S.salsa decreased by 41.1%,and the degree of landscape fragmentation increased from 1997 to 2020.Variations in the Largest Patch Index(LPI)indicated that the S.alterniflora landscape had become the dominant landscape type in the Yellow River Estuary.The res-ults of standard deviation ellipse(SDE)and Pearson’s correlation analyses indicated that a well-developed hydrological connectivity could promote the maintenance of the S.salsa landscape.The degradation of most S.salsa communities is caused by the influence of S.alterniflora on the morphological characteristics of the hydrological connectivity of tidal creek systems.
基金Supported by the National Natural Science Foundation of China(30670177)State Ocean863Project(2007AA091701)~~
文摘[Objective] Under salt stress condition,effects of inoculation of arbuscular mycorrhizal fungi Glomus mosseae on SOD and CAT activity in Suaeda salsa seedlings under salt stress were studied.[Method]There were 2 NaCl levels,namely 0 and 400 mmol/L and each NaCl contained 2 treatments,one is inoculated by Glomus mosseae and the other is control.The growths of Suaeda salsa,SOD and CAT activities as well as MDA content in leaves was determined.[Result]Under salt stress condition,Glomus mosseae could increase the growths of Suaeda salsa,SOD and CAT activities in leaves and decreased MDA content in leaves.[Conclusion]It preliminarily demonstrated that Arbuscular Mycorrhizal(AM)Fungi could increase salt resistance of Suaeda salsa by increasing the activities of SOD and CAT as well as alleviating membrane injury.
文摘AdoMet plays numerous roles of being the major methyl-group donor in trans-methylation reactions. To gain insight into the possible functions of the AdoMet protein of Suaeda salsa L. in response to salt stress, S adenosylmethionine synthetase gene (SAMS2) was analyzed. We isolated SAMS2 cDNA clone (AF321001) from a lambda -Zap cDNA library constructed from the halophyte S. salsa Pall aerial tissue treated with 400 mmol/L NaCl. SsSAMS2 was found to encode a S-adenolyl-L-methionine synthetase enzyme (AdoMet synthetase). The fragment was 1 531 bp with an open reading frame of 395 amino acids, the calculated molecular weight was about 43 kD. SsSAMS2 showed the highest homology to SAMS2 gene of Catharanthus roseus G. Don., with 93% identity in deduced amino acid sequence. Southern blotting analysis showed that SsSAMS2 might be a two-copy gene in S. salsa genome. Northern blot indicated that the cDNA was up-regulated by salt and other stresses. Enzyme activity assay indicated that the activity of SAMS2 increased under NaCl stress.
文摘Two different cDNA clones (Sscat1 and Sscat2) encoding catalase, the primary important H2O2-scavenging enzyme, were isolated from a AZap-cDNA library constructed from a 400 mmol/L NaCl-treated library of Suaeda salsa ( L.) Pall aerial tissue. Sscat1 (1.7 kb) contains a full open reading frame of 492 amino acids and Sscat2 (1.1 kb) is a partial clone. BLAST analysis indicates that the two clones share 71.9% identity in nucleotide sequence and 75% identity in deduced amino acid sequence within the last 287 amino acid residues of Sscat1. Southern blotting analysis showed that Sscat1 is multicopy in S. salsa genome, while Sscat2 is a single copy gene. Northern blotting analysis showed a rapid increase in the steady-level of both genes in roots after 48 It salt treatment, but only Sscat1 was induced in salinity treated leaves. Time-course analysis carried out in leaves confirmed that Sscat1 was induced by salt stress, in contrast to Sscat2. These implied that the expression of Sscat1 and Sscat2 genes are differentially regulated in S. salsa. The activity of total catalase is dramatically increased in response to salt stress.
文摘Suaeda salsa L. seedlings grown in Hoagland nutrient solution were treated with different concentrations of NaCl combined with two levels of K + (12 μmol/L and 6 mmol/L) to study the K + nutrition effect on plant growth and leaf tonoplast V-H +-ATPase and V-H +-PPase activity. Increase of K + supply in the culture solution markedly increased the fresh weight, dry weight and K + content of S. salsa plants. Western blot analysis showed that the leaf V-H +-ATPase of S. salsa was at least composed of A,B,C,D,E and c subunits, and their expression decreased with the increase of NaCl concentration under K + starvation (12 μmol/L K +), but increased under normal K + application (6 mmol/L K +). Leaf V-H +-PPase molecular weight was about 72.6 kD and its expression increased as NaCl concentration increased under both high or low levels of K + concentration in nutrient solution. There was a positive correlation between of V-H +-ATPase or V-H +-PPase activity and the amounts of their expression. Results in this study suggest that K + nutrition plays an important role in the salt tolerance of S. salsa, and K + is involved in the regulation of V-H +-ATPase or V-H +-PPase activity under salt stress.
文摘Introduction The plants of genus Cistanche G. Beck, which belong to Orobanchaceae family, include about twenty species, and there are four species and one variety are distributed in the northwest part of China. The dried fleshy stem of Cistanche genus plant known as ＇Roucongrong＇ in Chinese traditional medicine, has long been used for kidney deficiency, female infertility, morbid leucorrhea, and neurasthenia. Studies on active components and pharmacological activities show that it possesses significant activities in enhancing potency, anti-fatigue, immuno-modulability, etc, and phenylethanoid glycosides are the major active components. Due to excessive exploitation, its natural resources are facing with exhaustion. In order to improve this situation, the plant has been cultivated on a large scale in the northwest part of China. Up to now, the chemical investigations of the cultivated Cistartcbe salsa have not been undertaken. In this paper we report seven phenylethanoid glycosides from the cultivated Cistanche salsa： echinacoside （1）, cistanoside A （2）, acteoside （3）, isoacteoside （4）, 2＇-acetylacteoside （5）, tubuloside B （6）, and eutigoside A （7）. Among them, compound 7 isolated fronl family Orobanchaceae was reported previously, and compounds 1, 2, 3, 4, 5 and 6 from the cultivated Cistanche salsa are reported for the first time.
基金Supported by National Natural Science Foundation of China(40771172)Program for the Independent Innovation Team at State Key Laboratory of Resources and Environmental Information System(088RA400SA)Pilot Program of Knowledge Innovation Projectfrom Chinese Academy of Science~~
文摘[Objective] This study was to investigate the Suaeda salsa community characteristics,further getting the soil chemical properties.[Method] The paired-data of field spectra and corresponding soil physical-chemical property of seventeen samples was used to reveal the relationship between soil chemical property and field spectra（visible and near infra-red spectra）of S.salsa.[Result] The second derivative spectrum of S.salsa at 1 121 nm could reflect the changes of soil organic matter and soil total nitrogen,and that at 1 208 nm could commendably indicate changes of soil total phosphorus and at 724 nm could indicate changes of soil pH.The first derivative spectrum of S.salsa at 353 nm can indicate changes of soil available potassium,and that at 950 nm could commendably reflect the changes of soil salt content.[Conclusion] Our results laid basis for monitoring chemical property of soil covered with S.salsa using remote sensing technology.
基金国家重点基础研究发展规划(973)(the National Grand Fundamental Research973Program of China under Grant No.2007CB807902)国家自然科学基金(the National Natural Science Foundation of China under Grant No.90604036)国家杰出青年基金(the NationalScience Fund of China for Distinguished Young Scholar under Grant No.60225015)
