Global concerns about the environmental impact of combustion emissions from petroleum fuels influence new research to seek for alternative energy sources. The current study investigates the possibility of using safflo...Global concerns about the environmental impact of combustion emissions from petroleum fuels influence new research to seek for alternative energy sources. The current study investigates the possibility of using safflower (Carthamus tinctorius L.) as an alternative biodiesel raw material. Four plant growth regulators (PGR) were used to boost the production of safflower. Thirteen treatments were constituted from the four plant regulators and applied to the safflower crop arranged in completely randomised design, repeated three times. The results show that the effect of plant growth regulators was not more than that of the control. More studies have to be channelled towards the relationship between safflower and plant growth regulators.展开更多
[Objective] Study on the genetic diversity in main cultivars of safflower distributing in Xinjiang Uighur Autonomous Region by means of RAPD makers.[Method] Genomic DNAs of 29 safflower accessions from Xinjiang Uighur...[Objective] Study on the genetic diversity in main cultivars of safflower distributing in Xinjiang Uighur Autonomous Region by means of RAPD makers.[Method] Genomic DNAs of 29 safflower accessions from Xinjiang Uighur Autonomous Region were extracted for PCR amplification using 20 RAPD primers.[Result] Totally 156 bands were amplified,among which 144 bands were polymorphic(accounting for 92.31%),indicating that safflower is endowed with plentiful genetic diversity.Based on the DNA fingerprint,the 29 safflower accessions were grouped into four populations,the classification results may be not related with ecological regionality.[Conclusion] RAPD technique is an available tool to analyze the genetic diversity of safflower germplasm at molecular level.展开更多
Safflower is a popular Chinese medicinal plant and Safflower injection is extensively used for the clinical treatment of cerebrovascular and cardiovascular diseases. In this study, HPLC-DAD-ESI-MSn was utilized to stu...Safflower is a popular Chinese medicinal plant and Safflower injection is extensively used for the clinical treatment of cerebrovascular and cardiovascular diseases. In this study, HPLC-DAD-ESI-MSn was utilized to study the stability and degradation of the two major but chemically unstable bioactive compounds hydroxysaffior yellow A and anhydrosaffior yellow B, in Safflower injection. The impact of light irradiation, temperature, and pH on the stability of these two compounds were studied. The results showed that hydroxysafflor yellow A and anhydrosafflor yellow B could degrade at high temperature （〉60 ℃） or extreme pHs （pH ≤ 3.0 or 〉7.0）, but not under light irradiation. The common degradation product was p-coumaric acid. Chemical structures of the other degradation products were characterized by LC-MS. Hypothetical degradation pathways were proposed. In addition, ADP-induced platelet aggregation tests showed that the degradation of anhydrosaffior yellow B could reduce the anticoagulation activities of Safflower injection. Our results suggest that temperature and pH are critically important for the preparation and storage of Safflower injection.展开更多
We quantitatively determined four nucleosides, including cytidine, uridine, guanosine, and adenosine, in Carthamus tinctorius L. and Safflower injection. Separation was performed on a Zorbax Eclipse XDB-18 column usin...We quantitatively determined four nucleosides, including cytidine, uridine, guanosine, and adenosine, in Carthamus tinctorius L. and Safflower injection. Separation was performed on a Zorbax Eclipse XDB-18 column using a gradient elution with mobile phases of 0.05% trifluoroacetic acid （TFA） aqueous solution （A） and methanol （B）. The assay was carried out at a flow rate of 1 mL/min at 25 ℃ with detection at 260 nm. Cytidine, uridine, adenosine and guanosine showed good linearity in the ranges of4.02-503μg/mL （r2= 0.9998）, 9.38-1407 μg/mL （rz = 0.9999）, 80.6-8060μg/mL （r2 = 0.9999） and 2.10---630μg/mL （r2 = 0.9987） with average recoveries of 97.2%, 94.5%, 98.6% and 108.6%, respectively. The contents of cytidine, uridine, adenosine and guanosine in different Carthamus tinctorius L. and Safflower injection were significantly different. This is the first report on the quantitative determination of nucleosides in Carthamus tinctorius L. and Safflower injection.展开更多
An inductively coupled plasma mass spectrometry （ICP-MS） or inductively coupled plasma atomic emission spectrometry （ICP-AES） was developed to determine 19 elements in safflower, a traditional Chinese medicinal he...An inductively coupled plasma mass spectrometry （ICP-MS） or inductively coupled plasma atomic emission spectrometry （ICP-AES） was developed to determine 19 elements in safflower, a traditional Chinese medicinal herb from Xinjiang Autonomous Region and Henan Province of China. Totally 19 elements in safflower included heavy metals, i.e. As, Cd, Cu, Hg and Pb, and wholesome elements, i.e. Al, Ca, Co, Cr, Fe, Mg, Mn, Mo, Ni, P, Se, Sr, V and Zn. The results showed that the concentrations of heavy metals in safflower samples were both low, all of which met the national hygiene standards except for Pb in Xinjiang sample. Meanwhile, the distribution tendency of elements in the two samples was similar, which indicated that the plant might absorb given elements in a proportional way. The method can be used for the quality control of elements in safflower, and it provides a way for the determination of the contents of safflower from Xinjiang and Henan.展开更多
Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carri...Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carried out to investigate safflower regeneration through tissue culture and great progress had been made. Objective: To investigate factors that affect safflower regeneration through tissue culture principally. Methods: This article summarized available literatures about advancements in safflower regeneration, especially discussed factors affecting safflower tissue culture in detail. Results: Safflower regeneration was fairly hard than other congeneric plants, such as chrysanthemum. The genotype, seedling age, type of explants, medium components, plant growth regulators and other additives all had specific influences on safflower tissue culture. More deepgoing researches need to be undertaken to establish an effective safflower regeneration system.展开更多
Objective： To evaluate the clinical effect and safety of Safflower Yellow injection （SYI） in treating coronary heart disease angina pectoris （OHD-AP） with Xin-blood stagnation syndrome （XBSS）. Methods： Adopted...Objective： To evaluate the clinical effect and safety of Safflower Yellow injection （SYI） in treating coronary heart disease angina pectoris （OHD-AP） with Xin-blood stagnation syndrome （XBSS）. Methods： Adopted was the multi-centered, randomized, positive parallel controlled method, 448 patients with CHD-AP-XBSS were enrolled and divided into two groups, 336 in the tested group treated with SYI and 112 in the control group treated with Salvia injection by intravenous dripping once a day for 14 days, so as to observe the conditions of angina, electrocardiogram, and therapeutic effect on traditinal Chinese medicine （TCM） symptoms as well as the safety of the treatment. Results： The significantly effective rate and total effective rate in the tested group were 60.06% （194/323） and 91.02 % （294/323） respectively; those in improvement of TOM symptoms were 40. 18% （129/321） and 75.23% （243/323） respectively, which were better than those in the control group （P〈0.01）. Conclusion： SYI Injection is effective and safe in treating OHD-AP-XBSS.展开更多
OBJECTIVE： To evaluate the clinical efficacy of safflower yellow injection combined with conventional therapy in treating unstable angina pectoris.METHODS： We searched online databases： Chinese journal full-text da...OBJECTIVE： To evaluate the clinical efficacy of safflower yellow injection combined with conventional therapy in treating unstable angina pectoris.METHODS： We searched online databases： Chinese journal full-text database, China National Knowledge Infrastructure, Wanfang database, Chinese journal full-text database, Pubmed, ScienceDirect,Embase, and the Cochrane Library with manual-screening of relevant literature. Eligible randomized controlled trials（RCT） on angina pectoris were included. We conducted meta-analysis using the RevMan 5.1 software from The Cochrane Collaboration. We treated the relief rate of angina symptoms and electrocardiograph（ECG） as evaluation.RESULTS： Seven articles, including in 1134 patients, were enrolled after the evaluation. Therewas no significant heterogeneity among the studies（χ2=1.08, df=6, P=0.98, I2=0%）. The safflower yellow injection with conventional therapy has a higher effective rate than the control group in relieving the symptoms of angina pectoris [odds ratio（OR）=2.95, 95%（CI）（1.81, 4.81）] and improving ischemic ECG [OR=2.85, 95% CI（1.67, 4.86）]. The difference was statistically significant in the ＂80 mg dosage＂ and ＂100 mg dosage＂ subgroups（P0.05） for improving clinical symptoms and ECG. The funnel graphic was nearly symmetrical. Sensitivity analysis suggested that the results were stable.CONCLUSION： Safflower yellow injection as an adjunct therapy with conventional drugs shows advantages in easing the clinical symptoms of unstable angina and improving ECG over basic therapy alone. However, the conclusions should be interpreted with care until more high-quality RCTs are reported.展开更多
OBJECTIVE:To study the effects of hydroxy safflower yellow A(HSYA) on tumor capillary angiogenesis in transplanted human gastric adenocarcinoma BGC-823 tumors in nude mice.METHODS:BGC-823 cells were injected subcutane...OBJECTIVE:To study the effects of hydroxy safflower yellow A(HSYA) on tumor capillary angiogenesis in transplanted human gastric adenocarcinoma BGC-823 tumors in nude mice.METHODS:BGC-823 cells were injected subcutaneously into the right anterior armpit of nude mice to establish an animal model of transplanted tumors.After 24 h,18 nude mice injected with tumor cells were randomized into model,control,and HSYA 0.028 g/L groups,with six mice in each group.Transplanted tumors were excised on day 20.Tumor inhibition ratios were calculated for the transplanted tumors.Pathological changes and capillary angiogenesis in the tumors were observed by light microscopy.RESULTS:Tumors in the model group grew more quickly than those in the control and HSYA groups,with inhibition ratios of 48% and 30%,respectively.The microvessel count in the HSYA group was lower than in the model group(P<0.01),and microvessel density was also lower in the HSYA group(P<0.05).Pathological changes were more obvious in tumors in the model group compared to the HSYA group.CONCLUSION:HSYA inhibits the growth of transplanted BGC-823 tumors,and its effects on tumor capillary angiogenesis may represent one of the mechanisms responsible for this antineoplastic effect.展开更多
The total volatile components were extracted from safflower by ultrasonic-assisted solvent extraction （USE） and their chemical constituents were analyzed by gas chromatographymass spectrometry （GC-MS） to provide s...The total volatile components were extracted from safflower by ultrasonic-assisted solvent extraction （USE） and their chemical constituents were analyzed by gas chromatographymass spectrometry （GC-MS） to provide scientific basis for the quality control of safflower. Five different solvents （diethyl ether, ethanol, ethyl acetate, dichloromethane and acetone） were used and compared in terms of number of volatile components extracted and the peak areas of these components in TIC. The results showed that USE could be used as an efficient and rapid method for extracting the volatile components from safflower. It also could be found that the number of components in the TIC of ethyl acetate extract was more than that in the TIC of other solvent ones. Meanwhile, the volatile components of safflower from Xinjiang Autonomous Region and Henan Province of China were different in chemical components and relative contents. It could be concluded that both the extraction solvents and geographical origin of safflower are responsible for these differences. The experimental results also indicated that USE/GC-MS is a simple, rapid and effective method to analyze the volatile oil components of safflower.展开更多
Objective:Rapid discrimination of three classes of safflowers,dyed safflower,adulterated safflower,and pure safflower using computer vision and image processing algorithms.Methods:A low cost computer vision system(CVS...Objective:Rapid discrimination of three classes of safflowers,dyed safflower,adulterated safflower,and pure safflower using computer vision and image processing algorithms.Methods:A low cost computer vision system(CVS)was designed to measure the color of safflowers in the RGB(red,green,blue),L^*a^*b^*,and HSV(hue,saturation,vale)color spaces.The color moments in these three color spaces were extracted from the acquired images as color features of safflower.In addition,five kinds of pigments that are commonly used to dye safflowers were identified by high-performance liquid chromatography as a reference.Pattern recognition methods were investigated for rapid discrimination,including an unsupervised principal component analysis(PCA)algorithm and a supervised partial least squares discriminant analysis(PLS-DA)algorithm.Results:The mean error(e)between color values measured with the colorimeter and calculated with the CVS was 2.4%,with a high correlation coefficient(r)of 0.9905.This result indicated that the established CVS was reliable for color estimation of safflowers.The PLS-DA model,which had a total accuracy of 91.89%,outperformed the PCA model in classifying pure,adulterated,and dyed safflowers.Conclusion:The color objectification is a promising tool for rapid identification of dyed and adulterated safflowers.展开更多
Parkinson’s disease(PD) is a common degenerative disease of the central nervous system, and the pathologic features are mainly degeneration of substantianigra and dopamine neurons. Studies have shown that safflower f...Parkinson’s disease(PD) is a common degenerative disease of the central nervous system, and the pathologic features are mainly degeneration of substantianigra and dopamine neurons. Studies have shown that safflower flavonoid extract(SAFE) exhibits the neuroprotective effect. In this study, the safflower flavonoid extract drop pills(SAFE-DPs) for anti-PD were prepared by the heating and melting method using SAFE and matrix PEG6000. The performances of the pills were evaluated with powder X-ray diffraction(PXRD), differential scanning calorimetry(DSC), Fourier transform infrared spectroscopy(FT-IR), scanning electron microscopy(SEM) and dissolution testing. The analysis results demonstrated an amorphous state for SAFE dispersion in the matrix PEG6000 without any chemical reaction. The SAFE-DPs demonstrated acceptable chemical and physical stability irrespective of the manufacturing process and the storage period. Dissolution testing in three dissolution media(pH 1.0, pH 6.8 and pH 7.5) indicated that SAFE-DPs had excellent dissolution property. The transport of Kaempferol-3-rutinoside(K3 R) on the Caco-2 monolayer and the absorption of K3 R in situ intestinal perfusion revealed that the principal component of SAFE had a good transport and absorption capacity. Therefore, the drop pills had better release and absorption in the gastrointestinal tract, corresponding with the pharmacological and pharmacodynamic results for PD in vivo.展开更多
Safflower is widely cultivated and used as a dual-purpose medicinal oil worldwide.This research used the incoPat patent database to search the global safflower patents for nearly 20 years,aiming to reveal the developm...Safflower is widely cultivated and used as a dual-purpose medicinal oil worldwide.This research used the incoPat patent database to search the global safflower patents for nearly 20 years,aiming to reveal the development process and current situation of the safflower industry in China and around the world by analyzing patent application trends,patent technology characteristics,advantageous industries and regions,patent quality,etc.The analysis shows that safflower patent applications are generally increasing,and the period 2010-2016 is characterized by the rapid development of patent applications.The technical features of global patents are mainly concentrated in pharmaceutical preparations,cosmetics,food and health care,various beverages,animal feed,safflower cultivation and harvesting equipment,and safflower seed oil.During the rapid development of patent applications,safflower was further developed in cosmetics,edible oil,and harvesting equipment.In addition,patent applications in rheumatism and bone diseases have increased,with the potential for development.China is the leading region for researching and developing medicinal safflower patents,especially Shandong and Henan provinces.Foreign patents are more focused on the value development of safflower seed oil in food and chemicals.Compared to the major countries and organizations that apply for safflower patents on a global scale,the quality of Chinese safflower patents is medium,and more awareness of patent protection is required.This paper provides suggestions for the subsequent development of the safflower industry,summarizes and analyzes the development trend of safflower patents,and provides new ideas to obtain innovative patent results.展开更多
In this research we present that Carthamus Tinctorius L.(gen.Asteraceae,otherwise known as Safflower)(Fig.1)may contain agents active in Cryptococcal infections,malaria and Leishmaniasis,as treatment options are becom...In this research we present that Carthamus Tinctorius L.(gen.Asteraceae,otherwise known as Safflower)(Fig.1)may contain agents active in Cryptococcal infections,malaria and Leishmaniasis,as treatment options are becoming scarce due to drug resistance development.Phytochemistry and pharmacological activities(antimicrobial,antimalarial,antileishmanial)of C.tinctorius L.were analyzed.The composition of volatile oil of safflower dried flowers was analyzed by gas chromatography-mass spectrophotometry with flame ionization detector(GC-FID)and in vitro sensitivity assays were performed to assess biological activity.8 known and 3 unknown compounds were detected in the extract(Fig.1).Then the Safflower ointment was manufactured and its acute toxicity study on rats was tested.The volatile oil of C.tinctorius L exhibited activity against Cryptococcus neoformans,Plasmodium falciparum and Leishmania donovani.Safflower volatile oil has anticryptococcal,antimalarial and antileishmanial effects.The prepared ointment had an excellent acute toxicity safety profile.展开更多
Objective:To explore the best extraction technology of safflower polysaccharide by central composite design response surface methodology and evaluate its quality.Methods:Taking the extraction rate of Carthamus tinctor...Objective:To explore the best extraction technology of safflower polysaccharide by central composite design response surface methodology and evaluate its quality.Methods:Taking the extraction rate of Carthamus tinctorius polysaccharide as the index,taking the ratio of solid to liquid,extraction time,extraction temperature and extraction times as the investigation factors,based on the single factor experiment,the central composite design-response surface methodology was used to optimize the optimum extraction process of Carthamus tinctorius polysaccharide and verify it.Results:The response surface model was established with the extraction rate of Carthamus tinctorius polysaccharide as dependent variable Y,the ratio of solid to liquid,extraction time and extraction temperature as independent variables X,P<0.0001.The optimum extraction process was as follows:the ratio of solid to liquid was 1:16.69,the extraction temperature was 91.39℃,and the extraction working time was 89.78min.Under these conditions,the extraction rate of safflower polysaccharide can reach 7.45%,The experimental results show that RSD is 1.05%,and the model can well predict the experimental results.Conclusion:Central Composite Design-Response Surface Methodology has the advantages of high extraction rate,simple,effective and reasonable process operation,high stability and high precision,which can be fully applied to the resource management and utilization of safflower polysaccharide.展开更多
Safflower is an important, traditional, multipurpose oil crop. This was to investigate the effect of different salinity levels on morphological, physiological, biochemical and antioxidant response of two safflower cul...Safflower is an important, traditional, multipurpose oil crop. This was to investigate the effect of different salinity levels on morphological, physiological, biochemical and antioxidant response of two safflower cultivars (Carthamus tinctorius L. cultivar TSF1 and cultivar SM) differing in salt tolerance. Salinity stress (0.0%, 1.0%, 1.5% and 2.0% of NaCl) was induced to safflower plants after 19 days of vegetative growth. After 12 days of stress impositions, plants were harvested and analysed for various parameters. The results revealed that cultivar TSF1 showed maximum growth, dry weight, cell membrane stability and more water content in both root and leaf tissues at higher salinity levels than cultivar SM. Salt stress resulted an accumulation of more soluble sugars, amino acids, proline and glycine betaine at high salinity level confers the tolerance potential of cultivar TSF1 over cultivar SM. Salt stress induces more increase in the enzyme activity of superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase and catalase in tolerant cultivar than sensitive one. The results indicate that each cultivar adopt specific strategy at distinct salinity level for resistance against salinity. The possible conclusion is that improved tolerance in cultivar TSF1 to salinity may be accomplished by better management of growth, physiological attributes and antioxidative defence mechanisms.展开更多
Nutrient absorption in crops can decline and their development can be hindered by increased bulk density. This study aimed at assessing the manner in which bulk density levels affect the reproductive structures of the...Nutrient absorption in crops can decline and their development can be hindered by increased bulk density. This study aimed at assessing the manner in which bulk density levels affect the reproductive structures of the safflower genotypes in the Brazilian Cerrado. The completely randomized design was adopted with four replications for the experiment, which was conducted in a greenhouse using Oxisol collected from 0.0 to 0.2 m depth from the region supporting Cerrado vegetation. The treatments included ten safflower genotypes (PI 237538, PI 248385, PI 250196, PI 301049, PI 305173, PI 305205, PI 306520, PI 306603, PI 560202 and PI 613366) and five bulk density levels (1.0, 1.2, 1.4, 1.6 and 1.8 Mg·m-3). Evaluations were done at 90 days after emergence, in terms of the number, diameter and dry mass of the heads. The data were submitted to the analysis of variance. The means were grouped using the Scott-Knott test at 5% probability. The diameter and dry mass of the chapters were influenced by the mean bulk density of 1.10 Mg·m-3. A notable interaction was evident between the safflower genotypes and bulk density levels for the diameter and dry mass of the head alone, revealing the high degree of genetic variability that environmental changes induce among the genotypes. The PI 250196, PI 301049, PI 305173 and PI 305205 genotypes exhibited greater stability to the bulk density variations compared with the others. Mean bulk density of 1.2 Mg·m-3 was found to impair the development of the reproductive components of the safflower genotypes.展开更多
Weed management in safflower (Carthamus tinctorious L.) is a major challenge for growers due to very limited herbicide options available, particularly for broadleaf weed control. Field experiments were conducted at th...Weed management in safflower (Carthamus tinctorious L.) is a major challenge for growers due to very limited herbicide options available, particularly for broadleaf weed control. Field experiments were conducted at the Montana State University Southern Agricultural Research Center (MSU-SARC) near Huntley, MT in 2015 and 2016 to evaluate preemergence (PRE) soil-residual herbicides for crop safety and season-long broadleaf weed control in safflower. Among all herbicide programs tested, only sulfentrazone (105 g·ai·ha-1) alone or with pendimethalin (1064 g·ai·ha-1) caused 4% to 12% early-season visible injury to safflower, although the injury was not evident beyond 30 DAT. Sulfentrazone alone or with pendimethalin and pyroxasulfone (59 g·ai·ha-1) with pendimethalin had a season-long residual activity on kochia [Kochia scoparia (L.) Schrad] and Russian-thistle (Salsola tragus L), with 89% to 99% control at 60 DAT, and up to 98% reduction in weed density compared with dimethenamid-P (213 g·ai·ha-1) and S-metolachlor (433 g·ai·ha-1) at 65 DAT. Pyroxasulfone (59 or 118 g·ai·ha-1) alone or dimethenamid-P with pendimethalin provided a moderate to good control (65% to 79% at 60 DAT) of kochia and Russian-thistle. However, the end-season control of kochia or Russian-thistle was inadequate ( S-metolachlor alone program. Safflower grain yield with sulfentrazone alone or with pendimethalin, pyroxasulfone alone or with pendimethalin, and dimethenamid-P with pendimethalin averaged 3559 kg·ha-1, which was 195% higher compared with the nontreated check. In conclusion, sulfentrazone and pyroxasulfone or dimethenamid-P in combination with pendimethalin will be effective PRE herbicide programs for kochia and Russian-thistle control in safflower.展开更多
Optimistic and sustainable supply of soil available nutrients to crop plants enhances productivity. Integrated nutrient management (INM) approach can improve soil fertility on long term basis. The present study was co...Optimistic and sustainable supply of soil available nutrients to crop plants enhances productivity. Integrated nutrient management (INM) approach can improve soil fertility on long term basis. The present study was conducted to determine effects of INM on quantitative and qualitative characters of two Safflower (Carthamus tinctorius L.) cultivars “Thori-78 and Leed-00”. Five treatments using different composition of poultry litter, farm yard manure, nitrogen and phosphorous fertilizers with recommended dose as a control measure were replicated thrice in randomized complete design. The results of field trial depicted maximum plant height (174.6 cm), number of heads plant-1 (42.67), number of seeds head-1 (59.0), thousand seed weight (42.26 g), biological yield (3089 Kg·ha-1) and seed yield (455.2 Kg·ha-1) recorded from combined application of FYM @ 2 t·ha-1and Half (N-P) (soil application) (T5) in Genotype “Leed-00” which was statistically different from all other treatments. Thori-78 also showed increase in yield and yield components under the same treatment (T5) i.e.2 t·ha-1 FYM and Half (N-P).No effect of INM was found on fatty acid composition of safflower cultivars. The correlation coefficients illustrated positive and significant association of seed yield with plant height (0.89), number of heads pod-1 (0.86) and number of seeds head-1 (0.83) as a result of application of selected treatment. These results demonstrated the significance of INM in safflower yield improvement under rainfed conditions.展开更多
Development of stable crops cultivars adapted to environmental constraints is very important for food security. Safflower, an oilseed crop which tolerates environmental abiotic stresses, is suitable for marginal lands...Development of stable crops cultivars adapted to environmental constraints is very important for food security. Safflower, an oilseed crop which tolerates environmental abiotic stresses, is suitable for marginal lands relatively dry and deprived from fertilizer inputs or irrigation. A set of Moroccan and introduced cultivars as well as international accessions were conducted at Oujda （Eastern of Morocco） during 2009-2010 for late and conventional sowing under two water regimes, in a field experiment using a completely randomized design, with three replications. The objective was to evaluate the effect of genotype and contrasting environment on safflower behavior and to select genotypes with large adaptation to the contrasted environmental conditions. Morphological, physiological and agronomic traits, as well as the stress susceptibility index （SSI）, were recorded in this study. Results showed significant effect of genotype, year （sowing time）, water regime and their interaction on most of the studied parameters. Late sowing and drought affected negatively all the parameters except seed oil which lightly increased under drought stress. Number of heads per plant （NHP） had the strongest association with seed yield under both drought and non-drought conditions, and hence could be taken as selection criterion for safflower seed yield improvement. Five accessions showed the highest overall mean seed yield （~ 1,000 kg/ha） and four accessions exhibited the highest overall mean seed oil content （〉 310 g/kg）. For late sowing, the accessions P1262421 and PI537604 produced the highest seed yield （〉 800 kg/ha） and the highest seed oil content （〉 290 g/kg）. For conventional sowing, the accessions PI250076 and PI250523 were the most performant, with a seed yield 〉 1,300 kg/ha and a seed oil content 〉 330 g/kg. Based on their mean productivity across environments, their SSI and their MDA, P1271073 and P1250076 could be selected and used as promising germplasm in safflower breeding program in Morocco as well as other dry areas throughout the world.展开更多
文摘Global concerns about the environmental impact of combustion emissions from petroleum fuels influence new research to seek for alternative energy sources. The current study investigates the possibility of using safflower (Carthamus tinctorius L.) as an alternative biodiesel raw material. Four plant growth regulators (PGR) were used to boost the production of safflower. Thirteen treatments were constituted from the four plant regulators and applied to the safflower crop arranged in completely randomised design, repeated three times. The results show that the effect of plant growth regulators was not more than that of the control. More studies have to be channelled towards the relationship between safflower and plant growth regulators.
基金Supported by National Key Technology R&D Program(No2006BAI06A15-14s)~~
文摘[Objective] Study on the genetic diversity in main cultivars of safflower distributing in Xinjiang Uighur Autonomous Region by means of RAPD makers.[Method] Genomic DNAs of 29 safflower accessions from Xinjiang Uighur Autonomous Region were extracted for PCR amplification using 20 RAPD primers.[Result] Totally 156 bands were amplified,among which 144 bands were polymorphic(accounting for 92.31%),indicating that safflower is endowed with plentiful genetic diversity.Based on the DNA fingerprint,the 29 safflower accessions were grouped into four populations,the classification results may be not related with ecological regionality.[Conclusion] RAPD technique is an available tool to analyze the genetic diversity of safflower germplasm at molecular level.
基金Changjiang Scholar and Innovative Research Team in University (Grant No. 985-2-063-112)Youth Research Fellowship of Chinese Center for Disease Control and Prevention (Grant No. 2009A203)
文摘Safflower is a popular Chinese medicinal plant and Safflower injection is extensively used for the clinical treatment of cerebrovascular and cardiovascular diseases. In this study, HPLC-DAD-ESI-MSn was utilized to study the stability and degradation of the two major but chemically unstable bioactive compounds hydroxysaffior yellow A and anhydrosaffior yellow B, in Safflower injection. The impact of light irradiation, temperature, and pH on the stability of these two compounds were studied. The results showed that hydroxysafflor yellow A and anhydrosafflor yellow B could degrade at high temperature （〉60 ℃） or extreme pHs （pH ≤ 3.0 or 〉7.0）, but not under light irradiation. The common degradation product was p-coumaric acid. Chemical structures of the other degradation products were characterized by LC-MS. Hypothetical degradation pathways were proposed. In addition, ADP-induced platelet aggregation tests showed that the degradation of anhydrosaffior yellow B could reduce the anticoagulation activities of Safflower injection. Our results suggest that temperature and pH are critically important for the preparation and storage of Safflower injection.
基金Program for Changjiang Scholar and Innovative Team in University(Grant No.985-2-063-112)
文摘We quantitatively determined four nucleosides, including cytidine, uridine, guanosine, and adenosine, in Carthamus tinctorius L. and Safflower injection. Separation was performed on a Zorbax Eclipse XDB-18 column using a gradient elution with mobile phases of 0.05% trifluoroacetic acid （TFA） aqueous solution （A） and methanol （B）. The assay was carried out at a flow rate of 1 mL/min at 25 ℃ with detection at 260 nm. Cytidine, uridine, adenosine and guanosine showed good linearity in the ranges of4.02-503μg/mL （r2= 0.9998）, 9.38-1407 μg/mL （rz = 0.9999）, 80.6-8060μg/mL （r2 = 0.9999） and 2.10---630μg/mL （r2 = 0.9987） with average recoveries of 97.2%, 94.5%, 98.6% and 108.6%, respectively. The contents of cytidine, uridine, adenosine and guanosine in different Carthamus tinctorius L. and Safflower injection were significantly different. This is the first report on the quantitative determination of nucleosides in Carthamus tinctorius L. and Safflower injection.
文摘An inductively coupled plasma mass spectrometry （ICP-MS） or inductively coupled plasma atomic emission spectrometry （ICP-AES） was developed to determine 19 elements in safflower, a traditional Chinese medicinal herb from Xinjiang Autonomous Region and Henan Province of China. Totally 19 elements in safflower included heavy metals, i.e. As, Cd, Cu, Hg and Pb, and wholesome elements, i.e. Al, Ca, Co, Cr, Fe, Mg, Mn, Mo, Ni, P, Se, Sr, V and Zn. The results showed that the concentrations of heavy metals in safflower samples were both low, all of which met the national hygiene standards except for Pb in Xinjiang sample. Meanwhile, the distribution tendency of elements in the two samples was similar, which indicated that the plant might absorb given elements in a proportional way. The method can be used for the quality control of elements in safflower, and it provides a way for the determination of the contents of safflower from Xinjiang and Henan.
基金Supported by the National Natural Science Foundation of China Granted Project(81173484)
文摘Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carried out to investigate safflower regeneration through tissue culture and great progress had been made. Objective: To investigate factors that affect safflower regeneration through tissue culture principally. Methods: This article summarized available literatures about advancements in safflower regeneration, especially discussed factors affecting safflower tissue culture in detail. Results: Safflower regeneration was fairly hard than other congeneric plants, such as chrysanthemum. The genotype, seedling age, type of explants, medium components, plant growth regulators and other additives all had specific influences on safflower tissue culture. More deepgoing researches need to be undertaken to establish an effective safflower regeneration system.
文摘Objective： To evaluate the clinical effect and safety of Safflower Yellow injection （SYI） in treating coronary heart disease angina pectoris （OHD-AP） with Xin-blood stagnation syndrome （XBSS）. Methods： Adopted was the multi-centered, randomized, positive parallel controlled method, 448 patients with CHD-AP-XBSS were enrolled and divided into two groups, 336 in the tested group treated with SYI and 112 in the control group treated with Salvia injection by intravenous dripping once a day for 14 days, so as to observe the conditions of angina, electrocardiogram, and therapeutic effect on traditinal Chinese medicine （TCM） symptoms as well as the safety of the treatment. Results： The significantly effective rate and total effective rate in the tested group were 60.06% （194/323） and 91.02 % （294/323） respectively; those in improvement of TOM symptoms were 40. 18% （129/321） and 75.23% （243/323） respectively, which were better than those in the control group （P〈0.01）. Conclusion： SYI Injection is effective and safe in treating OHD-AP-XBSS.
基金Supported by Liaoning Province Science and Technology Plan Projects,Traditional Chinese Medicine Efficacy Evaluation Key Technology Research(No.2010225034)and(No.2010ZX09401-304)
文摘OBJECTIVE： To evaluate the clinical efficacy of safflower yellow injection combined with conventional therapy in treating unstable angina pectoris.METHODS： We searched online databases： Chinese journal full-text database, China National Knowledge Infrastructure, Wanfang database, Chinese journal full-text database, Pubmed, ScienceDirect,Embase, and the Cochrane Library with manual-screening of relevant literature. Eligible randomized controlled trials（RCT） on angina pectoris were included. We conducted meta-analysis using the RevMan 5.1 software from The Cochrane Collaboration. We treated the relief rate of angina symptoms and electrocardiograph（ECG） as evaluation.RESULTS： Seven articles, including in 1134 patients, were enrolled after the evaluation. Therewas no significant heterogeneity among the studies（χ2=1.08, df=6, P=0.98, I2=0%）. The safflower yellow injection with conventional therapy has a higher effective rate than the control group in relieving the symptoms of angina pectoris [odds ratio（OR）=2.95, 95%（CI）（1.81, 4.81）] and improving ischemic ECG [OR=2.85, 95% CI（1.67, 4.86）]. The difference was statistically significant in the ＂80 mg dosage＂ and ＂100 mg dosage＂ subgroups（P0.05） for improving clinical symptoms and ECG. The funnel graphic was nearly symmetrical. Sensitivity analysis suggested that the results were stable.CONCLUSION： Safflower yellow injection as an adjunct therapy with conventional drugs shows advantages in easing the clinical symptoms of unstable angina and improving ECG over basic therapy alone. However, the conclusions should be interpreted with care until more high-quality RCTs are reported.
文摘OBJECTIVE:To study the effects of hydroxy safflower yellow A(HSYA) on tumor capillary angiogenesis in transplanted human gastric adenocarcinoma BGC-823 tumors in nude mice.METHODS:BGC-823 cells were injected subcutaneously into the right anterior armpit of nude mice to establish an animal model of transplanted tumors.After 24 h,18 nude mice injected with tumor cells were randomized into model,control,and HSYA 0.028 g/L groups,with six mice in each group.Transplanted tumors were excised on day 20.Tumor inhibition ratios were calculated for the transplanted tumors.Pathological changes and capillary angiogenesis in the tumors were observed by light microscopy.RESULTS:Tumors in the model group grew more quickly than those in the control and HSYA groups,with inhibition ratios of 48% and 30%,respectively.The microvessel count in the HSYA group was lower than in the model group(P<0.01),and microvessel density was also lower in the HSYA group(P<0.05).Pathological changes were more obvious in tumors in the model group compared to the HSYA group.CONCLUSION:HSYA inhibits the growth of transplanted BGC-823 tumors,and its effects on tumor capillary angiogenesis may represent one of the mechanisms responsible for this antineoplastic effect.
文摘The total volatile components were extracted from safflower by ultrasonic-assisted solvent extraction （USE） and their chemical constituents were analyzed by gas chromatographymass spectrometry （GC-MS） to provide scientific basis for the quality control of safflower. Five different solvents （diethyl ether, ethanol, ethyl acetate, dichloromethane and acetone） were used and compared in terms of number of volatile components extracted and the peak areas of these components in TIC. The results showed that USE could be used as an efficient and rapid method for extracting the volatile components from safflower. It also could be found that the number of components in the TIC of ethyl acetate extract was more than that in the TIC of other solvent ones. Meanwhile, the volatile components of safflower from Xinjiang Autonomous Region and Henan Province of China were different in chemical components and relative contents. It could be concluded that both the extraction solvents and geographical origin of safflower are responsible for these differences. The experimental results also indicated that USE/GC-MS is a simple, rapid and effective method to analyze the volatile oil components of safflower.
基金the Special Fund of Beijing University of Chinese Medicine(Grant 2015-JYBXS111,to MX).
文摘Objective:Rapid discrimination of three classes of safflowers,dyed safflower,adulterated safflower,and pure safflower using computer vision and image processing algorithms.Methods:A low cost computer vision system(CVS)was designed to measure the color of safflowers in the RGB(red,green,blue),L^*a^*b^*,and HSV(hue,saturation,vale)color spaces.The color moments in these three color spaces were extracted from the acquired images as color features of safflower.In addition,five kinds of pigments that are commonly used to dye safflowers were identified by high-performance liquid chromatography as a reference.Pattern recognition methods were investigated for rapid discrimination,including an unsupervised principal component analysis(PCA)algorithm and a supervised partial least squares discriminant analysis(PLS-DA)algorithm.Results:The mean error(e)between color values measured with the colorimeter and calculated with the CVS was 2.4%,with a high correlation coefficient(r)of 0.9905.This result indicated that the established CVS was reliable for color estimation of safflowers.The PLS-DA model,which had a total accuracy of 91.89%,outperformed the PCA model in classifying pure,adulterated,and dyed safflowers.Conclusion:The color objectification is a promising tool for rapid identification of dyed and adulterated safflowers.
基金Science and Technology Major Projects:Significant New-Drugs Creation(Grant No.2012ZX09103201-042)
文摘Parkinson’s disease(PD) is a common degenerative disease of the central nervous system, and the pathologic features are mainly degeneration of substantianigra and dopamine neurons. Studies have shown that safflower flavonoid extract(SAFE) exhibits the neuroprotective effect. In this study, the safflower flavonoid extract drop pills(SAFE-DPs) for anti-PD were prepared by the heating and melting method using SAFE and matrix PEG6000. The performances of the pills were evaluated with powder X-ray diffraction(PXRD), differential scanning calorimetry(DSC), Fourier transform infrared spectroscopy(FT-IR), scanning electron microscopy(SEM) and dissolution testing. The analysis results demonstrated an amorphous state for SAFE dispersion in the matrix PEG6000 without any chemical reaction. The SAFE-DPs demonstrated acceptable chemical and physical stability irrespective of the manufacturing process and the storage period. Dissolution testing in three dissolution media(pH 1.0, pH 6.8 and pH 7.5) indicated that SAFE-DPs had excellent dissolution property. The transport of Kaempferol-3-rutinoside(K3 R) on the Caco-2 monolayer and the absorption of K3 R in situ intestinal perfusion revealed that the principal component of SAFE had a good transport and absorption capacity. Therefore, the drop pills had better release and absorption in the gastrointestinal tract, corresponding with the pharmacological and pharmacodynamic results for PD in vivo.
基金the National Famous and Old Chinese Medicine Expert Inheritance Studio of the State Administration of Traditional Chinese Medicine([2019]No.41)The key research and development projects of Sichuan Science and Technology Plan(2020YFN0152,2021YFYZ0012-5)+1 种基金Chengdu University of Traditional Chinese Medicine"Xinglin Scholars"Discipline Talents Research Promotion Program(CXTD2018003,QJRC2022025)Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(ZYYCXTD-D-202209).
文摘Safflower is widely cultivated and used as a dual-purpose medicinal oil worldwide.This research used the incoPat patent database to search the global safflower patents for nearly 20 years,aiming to reveal the development process and current situation of the safflower industry in China and around the world by analyzing patent application trends,patent technology characteristics,advantageous industries and regions,patent quality,etc.The analysis shows that safflower patent applications are generally increasing,and the period 2010-2016 is characterized by the rapid development of patent applications.The technical features of global patents are mainly concentrated in pharmaceutical preparations,cosmetics,food and health care,various beverages,animal feed,safflower cultivation and harvesting equipment,and safflower seed oil.During the rapid development of patent applications,safflower was further developed in cosmetics,edible oil,and harvesting equipment.In addition,patent applications in rheumatism and bone diseases have increased,with the potential for development.China is the leading region for researching and developing medicinal safflower patents,especially Shandong and Henan provinces.Foreign patents are more focused on the value development of safflower seed oil in food and chemicals.Compared to the major countries and organizations that apply for safflower patents on a global scale,the quality of Chinese safflower patents is medium,and more awareness of patent protection is required.This paper provides suggestions for the subsequent development of the safflower industry,summarizes and analyzes the development trend of safflower patents,and provides new ideas to obtain innovative patent results.
文摘In this research we present that Carthamus Tinctorius L.(gen.Asteraceae,otherwise known as Safflower)(Fig.1)may contain agents active in Cryptococcal infections,malaria and Leishmaniasis,as treatment options are becoming scarce due to drug resistance development.Phytochemistry and pharmacological activities(antimicrobial,antimalarial,antileishmanial)of C.tinctorius L.were analyzed.The composition of volatile oil of safflower dried flowers was analyzed by gas chromatography-mass spectrophotometry with flame ionization detector(GC-FID)and in vitro sensitivity assays were performed to assess biological activity.8 known and 3 unknown compounds were detected in the extract(Fig.1).Then the Safflower ointment was manufactured and its acute toxicity study on rats was tested.The volatile oil of C.tinctorius L exhibited activity against Cryptococcus neoformans,Plasmodium falciparum and Leishmania donovani.Safflower volatile oil has anticryptococcal,antimalarial and antileishmanial effects.The prepared ointment had an excellent acute toxicity safety profile.
基金National Natural Science Foundation of China(No.81603418)General Program of Natural Science Foundation of Heilongjiang(No.H201472)Project of Excellent Youth Talents of Heilongjiang(No.2020YQ05)。
文摘Objective:To explore the best extraction technology of safflower polysaccharide by central composite design response surface methodology and evaluate its quality.Methods:Taking the extraction rate of Carthamus tinctorius polysaccharide as the index,taking the ratio of solid to liquid,extraction time,extraction temperature and extraction times as the investigation factors,based on the single factor experiment,the central composite design-response surface methodology was used to optimize the optimum extraction process of Carthamus tinctorius polysaccharide and verify it.Results:The response surface model was established with the extraction rate of Carthamus tinctorius polysaccharide as dependent variable Y,the ratio of solid to liquid,extraction time and extraction temperature as independent variables X,P<0.0001.The optimum extraction process was as follows:the ratio of solid to liquid was 1:16.69,the extraction temperature was 91.39℃,and the extraction working time was 89.78min.Under these conditions,the extraction rate of safflower polysaccharide can reach 7.45%,The experimental results show that RSD is 1.05%,and the model can well predict the experimental results.Conclusion:Central Composite Design-Response Surface Methodology has the advantages of high extraction rate,simple,effective and reasonable process operation,high stability and high precision,which can be fully applied to the resource management and utilization of safflower polysaccharide.
文摘Safflower is an important, traditional, multipurpose oil crop. This was to investigate the effect of different salinity levels on morphological, physiological, biochemical and antioxidant response of two safflower cultivars (Carthamus tinctorius L. cultivar TSF1 and cultivar SM) differing in salt tolerance. Salinity stress (0.0%, 1.0%, 1.5% and 2.0% of NaCl) was induced to safflower plants after 19 days of vegetative growth. After 12 days of stress impositions, plants were harvested and analysed for various parameters. The results revealed that cultivar TSF1 showed maximum growth, dry weight, cell membrane stability and more water content in both root and leaf tissues at higher salinity levels than cultivar SM. Salt stress resulted an accumulation of more soluble sugars, amino acids, proline and glycine betaine at high salinity level confers the tolerance potential of cultivar TSF1 over cultivar SM. Salt stress induces more increase in the enzyme activity of superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase and catalase in tolerant cultivar than sensitive one. The results indicate that each cultivar adopt specific strategy at distinct salinity level for resistance against salinity. The possible conclusion is that improved tolerance in cultivar TSF1 to salinity may be accomplished by better management of growth, physiological attributes and antioxidative defence mechanisms.
文摘Nutrient absorption in crops can decline and their development can be hindered by increased bulk density. This study aimed at assessing the manner in which bulk density levels affect the reproductive structures of the safflower genotypes in the Brazilian Cerrado. The completely randomized design was adopted with four replications for the experiment, which was conducted in a greenhouse using Oxisol collected from 0.0 to 0.2 m depth from the region supporting Cerrado vegetation. The treatments included ten safflower genotypes (PI 237538, PI 248385, PI 250196, PI 301049, PI 305173, PI 305205, PI 306520, PI 306603, PI 560202 and PI 613366) and five bulk density levels (1.0, 1.2, 1.4, 1.6 and 1.8 Mg·m-3). Evaluations were done at 90 days after emergence, in terms of the number, diameter and dry mass of the heads. The data were submitted to the analysis of variance. The means were grouped using the Scott-Knott test at 5% probability. The diameter and dry mass of the chapters were influenced by the mean bulk density of 1.10 Mg·m-3. A notable interaction was evident between the safflower genotypes and bulk density levels for the diameter and dry mass of the head alone, revealing the high degree of genetic variability that environmental changes induce among the genotypes. The PI 250196, PI 301049, PI 305173 and PI 305205 genotypes exhibited greater stability to the bulk density variations compared with the others. Mean bulk density of 1.2 Mg·m-3 was found to impair the development of the reproductive components of the safflower genotypes.
文摘Weed management in safflower (Carthamus tinctorious L.) is a major challenge for growers due to very limited herbicide options available, particularly for broadleaf weed control. Field experiments were conducted at the Montana State University Southern Agricultural Research Center (MSU-SARC) near Huntley, MT in 2015 and 2016 to evaluate preemergence (PRE) soil-residual herbicides for crop safety and season-long broadleaf weed control in safflower. Among all herbicide programs tested, only sulfentrazone (105 g·ai·ha-1) alone or with pendimethalin (1064 g·ai·ha-1) caused 4% to 12% early-season visible injury to safflower, although the injury was not evident beyond 30 DAT. Sulfentrazone alone or with pendimethalin and pyroxasulfone (59 g·ai·ha-1) with pendimethalin had a season-long residual activity on kochia [Kochia scoparia (L.) Schrad] and Russian-thistle (Salsola tragus L), with 89% to 99% control at 60 DAT, and up to 98% reduction in weed density compared with dimethenamid-P (213 g·ai·ha-1) and S-metolachlor (433 g·ai·ha-1) at 65 DAT. Pyroxasulfone (59 or 118 g·ai·ha-1) alone or dimethenamid-P with pendimethalin provided a moderate to good control (65% to 79% at 60 DAT) of kochia and Russian-thistle. However, the end-season control of kochia or Russian-thistle was inadequate ( S-metolachlor alone program. Safflower grain yield with sulfentrazone alone or with pendimethalin, pyroxasulfone alone or with pendimethalin, and dimethenamid-P with pendimethalin averaged 3559 kg·ha-1, which was 195% higher compared with the nontreated check. In conclusion, sulfentrazone and pyroxasulfone or dimethenamid-P in combination with pendimethalin will be effective PRE herbicide programs for kochia and Russian-thistle control in safflower.
文摘Optimistic and sustainable supply of soil available nutrients to crop plants enhances productivity. Integrated nutrient management (INM) approach can improve soil fertility on long term basis. The present study was conducted to determine effects of INM on quantitative and qualitative characters of two Safflower (Carthamus tinctorius L.) cultivars “Thori-78 and Leed-00”. Five treatments using different composition of poultry litter, farm yard manure, nitrogen and phosphorous fertilizers with recommended dose as a control measure were replicated thrice in randomized complete design. The results of field trial depicted maximum plant height (174.6 cm), number of heads plant-1 (42.67), number of seeds head-1 (59.0), thousand seed weight (42.26 g), biological yield (3089 Kg·ha-1) and seed yield (455.2 Kg·ha-1) recorded from combined application of FYM @ 2 t·ha-1and Half (N-P) (soil application) (T5) in Genotype “Leed-00” which was statistically different from all other treatments. Thori-78 also showed increase in yield and yield components under the same treatment (T5) i.e.2 t·ha-1 FYM and Half (N-P).No effect of INM was found on fatty acid composition of safflower cultivars. The correlation coefficients illustrated positive and significant association of seed yield with plant height (0.89), number of heads pod-1 (0.86) and number of seeds head-1 (0.83) as a result of application of selected treatment. These results demonstrated the significance of INM in safflower yield improvement under rainfed conditions.
文摘Development of stable crops cultivars adapted to environmental constraints is very important for food security. Safflower, an oilseed crop which tolerates environmental abiotic stresses, is suitable for marginal lands relatively dry and deprived from fertilizer inputs or irrigation. A set of Moroccan and introduced cultivars as well as international accessions were conducted at Oujda （Eastern of Morocco） during 2009-2010 for late and conventional sowing under two water regimes, in a field experiment using a completely randomized design, with three replications. The objective was to evaluate the effect of genotype and contrasting environment on safflower behavior and to select genotypes with large adaptation to the contrasted environmental conditions. Morphological, physiological and agronomic traits, as well as the stress susceptibility index （SSI）, were recorded in this study. Results showed significant effect of genotype, year （sowing time）, water regime and their interaction on most of the studied parameters. Late sowing and drought affected negatively all the parameters except seed oil which lightly increased under drought stress. Number of heads per plant （NHP） had the strongest association with seed yield under both drought and non-drought conditions, and hence could be taken as selection criterion for safflower seed yield improvement. Five accessions showed the highest overall mean seed yield （~ 1,000 kg/ha） and four accessions exhibited the highest overall mean seed oil content （〉 310 g/kg）. For late sowing, the accessions P1262421 and PI537604 produced the highest seed yield （〉 800 kg/ha） and the highest seed oil content （〉 290 g/kg）. For conventional sowing, the accessions PI250076 and PI250523 were the most performant, with a seed yield 〉 1,300 kg/ha and a seed oil content 〉 330 g/kg. Based on their mean productivity across environments, their SSI and their MDA, P1271073 and P1250076 could be selected and used as promising germplasm in safflower breeding program in Morocco as well as other dry areas throughout the world.
