为了探讨热激蛋白在低温锻炼诱导的耐热性形成过程中的保护功能,进一步分析葡萄幼苗对温度逆境产生交叉适应性的细胞学机制,试验以葡萄(Vitis vinifera L. cv. Jingxiu)幼苗为试材,采用胶体金免疫电镜定位技术,定位观察了小分子热激蛋白...为了探讨热激蛋白在低温锻炼诱导的耐热性形成过程中的保护功能,进一步分析葡萄幼苗对温度逆境产生交叉适应性的细胞学机制,试验以葡萄(Vitis vinifera L. cv. Jingxiu)幼苗为试材,采用胶体金免疫电镜定位技术,定位观察了小分子热激蛋白17.6(sHSP17.6)在葡萄叶片中的亚细胞分布情况,结果表明,低温锻炼预处理可增强其在高温胁迫条件下的表达水平,细胞核和叶绿体中代表sHSP17.6的免疫金颗粒密度比相应的对照细胞明显增多,尤其是胁迫处理3 h时。这一结果为sHSP17.6参与低温锻炼诱导的耐热性提供了更直观的细胞学证据。展开更多
Small heat shock protei ns(sHSPs)are a very complex protei n superfamily that increase in sect temperature tolera nee.In order to deeply understand the function and role of sHSPs in Chilo suppressalis(Walker),this stu...Small heat shock protei ns(sHSPs)are a very complex protei n superfamily that increase in sect temperature tolera nee.In order to deeply understand the function and role of sHSPs in Chilo suppressalis(Walker),this study isolated and identified two CsHSP genes lacking introns from C.suppressalis,Cshsp23.9 and Cshsp27.3.The cDNA full-length of Cshsp23.9 and Cshsp27.3 were 909 and 1036 bp encoding 220 and 242 amino acids,respectively.Alignment with homologs and phylogenetic analysis indicated Cshsp23.9 and Cshsp27.3 were two new types of Cshsps in C.suppressalis.Real-time quantitative PCR(qPCR)revealed that Cshsp23.9 had the highest relative expression in hindgut compared with other tissues(head,epidermis,foregut,midgut,fat body,Malpighian tubules,and hemocytes),while Cs/?sp27.3 expressed the highest in fat body con tent.Whe n exposed to thermal stress from-11 to 43°C for 2 h,two genes showed differe nt expression patterns.Cshsp23.9 did not respond to low temperature,but could be up-regulated by high temperature and the highest expression temperature was at 36°C.Cshsp27.3 could only be induced by mild temperature,with the highest expression at 15 and 30°C.In conclusion,Cshsp23.9 and Cshsp27.3 existed in different tissues/organs of C.suppressalis,and played different important roles in C.suppressalis to resist temperature stress and regulate physiological activities.展开更多
基金This research was funded by the National Natural Science Foundation of China(31401733 and 31371937)and the National Basic Research and Development Program of China(2013CB127604).We sincerely thank Dr.Tom McAvoy from Virginia Tech,USA for editing on this manu script.
文摘Small heat shock protei ns(sHSPs)are a very complex protei n superfamily that increase in sect temperature tolera nee.In order to deeply understand the function and role of sHSPs in Chilo suppressalis(Walker),this study isolated and identified two CsHSP genes lacking introns from C.suppressalis,Cshsp23.9 and Cshsp27.3.The cDNA full-length of Cshsp23.9 and Cshsp27.3 were 909 and 1036 bp encoding 220 and 242 amino acids,respectively.Alignment with homologs and phylogenetic analysis indicated Cshsp23.9 and Cshsp27.3 were two new types of Cshsps in C.suppressalis.Real-time quantitative PCR(qPCR)revealed that Cshsp23.9 had the highest relative expression in hindgut compared with other tissues(head,epidermis,foregut,midgut,fat body,Malpighian tubules,and hemocytes),while Cs/?sp27.3 expressed the highest in fat body con tent.Whe n exposed to thermal stress from-11 to 43°C for 2 h,two genes showed differe nt expression patterns.Cshsp23.9 did not respond to low temperature,but could be up-regulated by high temperature and the highest expression temperature was at 36°C.Cshsp27.3 could only be induced by mild temperature,with the highest expression at 15 and 30°C.In conclusion,Cshsp23.9 and Cshsp27.3 existed in different tissues/organs of C.suppressalis,and played different important roles in C.suppressalis to resist temperature stress and regulate physiological activities.
