The corrosion behavior of a rusted 550 MPa grade offshore platform steel in Clcontaining environment was investigated.The results revealed that the corrosion process can be divided into initial stage in which corrosio...The corrosion behavior of a rusted 550 MPa grade offshore platform steel in Clcontaining environment was investigated.The results revealed that the corrosion process can be divided into initial stage in which corrosion rate increased with accumulation of corrosion products and later stage in which homogeneous and compact rust layer started to protect steel substrate out of corrosion mediums.On the contrary,structural analysis of rust layers by X-ray diffraction showed that α-FeOOH increased from 1.3% to 3.6% and the Fe3O4 increased from 1.0% to 1.5% while γ-FeOOH reduced slightly according to corrosion time increased from 30 cycles to 73 cycles.The results of electron probe microanalysis indicated that Cr concentrated mainly in the inner region of the rust,inner/outer interface especially,whereas Ni and Cu were uniformly distributed all over the rust after 73 corrosion cycles.According to electrochemical measurements,it was found that the corrosion rate of rusted steel reduced from 0.61 mm/a after 45 cycles to 0.34 mm/a after 85 cycles,44.3% reduction approximately,and Rrust values increased with increment of corrosion time.Therefore,formation of compact inner rust layer and enrichment of Cr are important to improve corrosion resistance of offshore platform steel.展开更多
The relationship between the specific surface area（SSA） of rust and the electrochemical behavior of rusted steel under wet-dry acid corrosion conditions was investigated. The results showed that the corrosion curren...The relationship between the specific surface area（SSA） of rust and the electrochemical behavior of rusted steel under wet-dry acid corrosion conditions was investigated. The results showed that the corrosion current density first increased and then decreased with increasing SSA of the rust during the corrosion process. The structure of the rust changed from single-layer to double-layer, and the γ-FeOOH content decreased in the inner layer of the rust with increasing corrosion time; by contrast, the γ-FeOOH content in the outer layer was constant. When the SSA of the rust was lower than the critical SSA corresponding to the relative humidity during the drying period, condensed water in the micropores of the rust could evaporate, which prompted the diffusion of O_2 into the rust and the following formation process of γ-FeOOH, leading to an increase of corrosion current density with increasing corrosion time. However, when the SSA of the rust reached or exceeded the critical SSA, condensate water in the micro-pores of the inner layer of the rust could not evaporate which inhibited the diffusion of O_2 and decreased the γ-FeOOH content in the inner rust, leading to a decrease of corrosion current density with increasing corrosion time.展开更多
The biotrophic fungus Puccinia striiformis f. sp. tritici is the causal agent of the yellow rust in wheat. Between the years 2010–2013 a new strain of this pathogen(Warrior/Ambition),against which the present cultiva...The biotrophic fungus Puccinia striiformis f. sp. tritici is the causal agent of the yellow rust in wheat. Between the years 2010–2013 a new strain of this pathogen(Warrior/Ambition),against which the present cultivated wheat varieties have no resistance, appeared and spread rapidly. It threatens cereal production in most of Europe. The search for sources of resistance to this strain is proposed as the most efficient and safe solution to ensure high grain production. This will be helped by the development of high performance and low cost techniques for field phenotyping. In this study we analyzed vegetation indices in the Red,Green, Blue(RGB) images of crop canopies under field conditions. We evaluated their accuracy in predicting grain yield and assessing disease severity in comparison to other field measurements including the Normalized Difference Vegetation Index(NDVI), leaf chlorophyll content, stomatal conductance, and canopy temperature. We also discuss yield components and agronomic parameters in relation to grain yield and disease severity.RGB-based indices proved to be accurate predictors of grain yield and grain yield losses associated with yellow rust(R2= 0.581 and R2= 0.536, respectively), far surpassing the predictive ability of NDVI(R2= 0.118 and R2= 0.128, respectively). In comparison to potential yield, we found the presence of disease to be correlated with reductions in the number of grains per spike, grains per square meter, kernel weight and harvest index. Grain yield losses in the presence of yellow rust were also greater in later heading varieties. The combination of RGB-based indices and days to heading together explained 70.9% of the variability in grain yield and 62.7% of the yield losses.展开更多
An eco-friendly rust converter treatment based on tannic acid was optimized by orthogonal experiment, and the adhesion of the epoxy top-coating system was evaluated. The results showed that the adhesion between the ep...An eco-friendly rust converter treatment based on tannic acid was optimized by orthogonal experiment, and the adhesion of the epoxy top-coating system was evaluated. The results showed that the adhesion between the epoxy coating and the rusted steel after treatment was enhanced from 1.93 to 5.97 MPa. This adhesion improvement was attributed to the formation of the micro-cracked tannic conversion layer and the increase of the compactness of the rust layer. The failure analysis suggested that the remainder rust was still the key issue for the further adhesion improvement of the coating system.展开更多
Thinopyrum ponticum(2n=10×=70),a wild relative of common wheat(Triticum aestivum L.),is considered an invaluable genetic resource for wheat improvement due to its abundance of genes conferring resistance to bioti...Thinopyrum ponticum(2n=10×=70),a wild relative of common wheat(Triticum aestivum L.),is considered an invaluable genetic resource for wheat improvement due to its abundance of genes conferring resistance to biotic and abiotic stresses.This study focused on the CH97 line,derived from the BC1F7 progeny of a cross between wheat cv.7182 and Th.ponticum.Cytological evidence showed that CH97 has 42 chromosomes,forming 21 bivalents at meiotic metaphase I,with the bivalents subsequently separating and moving to opposite poles during meiotic anaphase I.Through a combination of fluorescence in situ hybridization(FISH),genomic in situ hybridization(GISH),multicolor GISH(mc-GISH),and liquid array analysis,it was determined that CH97 comprises 40 wheat chromosomes and two alien chromosomes from the Ee genome of Th.ponticum,featuring the absence of a pair of 5D chromosomes and variations in 1B,6B,and 7B chromosomes.These findings confirm that CH97 is a stable wheat-Th.ponticum 5E(5D)alien disomic substitution line.Inoculation experiments revealed that CH97 exhibits high resistance to wheat powdery mildew and stripe rust throughout the growth period,in contrast to the highly susceptible common wheat parent 7182.Compared to 7182,CH97 displayed improvements in thousand-kernel weight and kernel length.Additionally,utilizing specific-locus amplified fragment sequencing(SLAF-seq)technology,chromosome 5E-specific molecular markers were developed and validated,achieving a 33.3% success rate,facilitating marker-assisted selection for disease resistance in wheat.Overall,the CH97 substitution line,with its resistance to diseases and improved agronomic traits,represents valuable new germplasm for wheat chromosome engineering and breeding.展开更多
In China,Xinjiang is a relatively independent epidemic region of wheat stripe rust,caused by Puccinia striiformis f.sp.tritici,due to great population genetic divergence of Xinjiang with other inland epidemic regions....In China,Xinjiang is a relatively independent epidemic region of wheat stripe rust,caused by Puccinia striiformis f.sp.tritici,due to great population genetic divergence of Xinjiang with other inland epidemic regions.In this region,race evolution was usually slower than inland populations.However,many new races have recently been found,and therefore,it is necessary for more understanding of the virulence evolution of the Xinjiang population.So,in this study,a 65 sexual progenies,derived from a Xinjiang single-urediospore isolate BGTB-1 of P.striiformis f.sp.tritici by selfing on alternate host barberry(Berberis aggregata).It was phenotyped on the 25 single Yr lines,and genotyped by 19 kompetitive allele-specific PCRsingle nucleotide polymorphism(KASP-SNP) markers.As a result,the 65 progenies were identified as 56 various virulence patterns(VPs),and neither of which was identical to the parental isolate,showed 100% virulence variation.Compared with the parental isolate,of all progenies,39(60.0%) had increased virulence,and 18(27.7%) had decreased virulence.All progenies exhibited avirulence to Yr10,Yr15,Yr32,and YrTr1 loci,and avirulence and virulence segregation at the remaining 21 Yr resistance loci.The results showed avirulence to Yr5,Yr7,and Yr76(A:V≈3:1) loci is controlled by a single dominant gene,and that to Yr6,Yr25,and Yr44(A:V≈1:3) loci by a single recessive gene.However,avirulence to the remaining 15 resistant loci including Yr1,Yr2,Yr3,Yr4,Yr8,Yr9,Yr17,Yr26(=Yr24),Yr28,Yr29,Yr43,YrSP,Yr27,YrA,and YrExp2,with various avirulence and virulence segregation ratios,is controlled by two genes with different gene effects,indicating complex genetic traits of the parental isolate.Totally,65 dissimilar genotypes detected among progenies using overall molecular markers,by which a linkage map was constructed,with a genetic distance of 441.0 cM.Interestingly,although the parental isolate was avirulent to Yr5,but 17 progenies showed virulence,showing the change of pathogenicity from avirulence to virulence at this resistance locus.It was for the first time to report that progenies with virulence to Yr5 produced sexually from avirulent parental isolate at this resistance locus.To our knowledge,this study offers an insight into inheritance,sexual reproduction and virulence variation of P.striiformis f.sp.tritici in Xinjiang,facilitating us to understand evolution of the rust pathogen in this region and accounting for Xinjiang population distinguished form other inland populations.Additionally,it is necessary to further confirm the roles of sexual reproduction in the emergence of new races and affecting population genetic diversity of P.striiformis f.sp.tritici under natural conditions in Xinjiang.展开更多
Waterborne acrylic coatings are widely utilized due to their cost-effectiveness,high transparency,strong resistance to weather and chemicals,impressive mechanical properties,and excellent adhesion to various substrate...Waterborne acrylic coatings are widely utilized due to their cost-effectiveness,high transparency,strong resistance to weather and chemicals,impressive mechanical properties,and excellent adhesion to various substrates.In these coatings,a reactive emulsifier containing phosphate groups can be integrated into the molecular chain during polymerization,which enhances the coating's compactness and corrosion resistance.This work focuses on the synthesis of styrene-butyl acrylate(St-BA)latex and methyl methacrylate-butyl acrylate(MMA-BA)latex using the reactive phosphate emulsifier ANPEO_(10)-P_(1) through seed emulsion polymerization,achieving a conversion rate of approximately 99%and a solid content close to 50%.The resulting coatings from St-BA and MMA-BA latexes demonstrated long-term corrosion protection for carbon steel and aluminum alloy due to in-situ phosphatization,effectively preventing flash rust.Notably,the MMA-BA coating exhibited remarkable durability,enduring immersion for up to 1224 h(51 d)on Q 235 carbon steel before reaching the failure threshold(|Z|0.01 Hz£106Ω·cm^(2))on Q 235 carbon steel.On 5052 aluminum alloy,the St-BA coating maintained|Z|0.01 Hz>10^(8)Ω·cm^(2) for 480 h(20 d).Furthermore,the corrosion resistance of St-BA and MMA BA coatings on Q 235 steel sheet and 5052 aluminum alloy surpassed that of commercially available MMA-BA and St BA coatings after immersion in a 3.5 wt%NaCl aqueous solution.This work also delves into the anticorrosion mechanism of MMA-BA and St-BA coatings.展开更多
The crevice corrosion of the reinforcing steel in the carbonated simulated concrete pore solutions(SCPSs)containing 0.04 mol/L chloride was investigated.In comparison,the steel without crevice covered on its surface w...The crevice corrosion of the reinforcing steel in the carbonated simulated concrete pore solutions(SCPSs)containing 0.04 mol/L chloride was investigated.In comparison,the steel without crevice covered on its surface was also studied.Results showed that the crevice corrosion in the uncarbonated SCPS was not triggered and the steel remained passivity by suppressing the anodic dissolution in the crevice interior.As SCPS was carbonated to have pH values of 10.5 and 11.5,the crevice corrosion was easier to be activated than the widely reported pitting corrosion owing to the rapid oxygen depletion within the crevice,but the crevice corrosion damage might be alleviated to a certain extent due to the relative compact surface film formed inside the crevice.The further carbonation led to the activation dissolution of both the crevice interior and exterior,causing the more serious corrosion damage.Corrosion process of the crevice corrosion was systematically analyzed by combining the electrochemical measurement results and Evens polarization diagram.展开更多
The rust layer is a critical factor in determining the corrosion resistance performance of weathering bridge steel.Understanding the evolution mechanism of this rust layer is fundamental for the design and optimizatio...The rust layer is a critical factor in determining the corrosion resistance performance of weathering bridge steel.Understanding the evolution mechanism of this rust layer is fundamental for the design and optimization of such steel.This study investigates the evolu-tion of the rust layer on high-Cr-content weathering bridge steel,using an atmospheric corrosion monitoring(ACM)sensor and big data mining techniques in a simulated tropical marine atmosphere.Results reveal that the protective properties of the rust layer follow a peri-odic pattern of“ascending–constant”rather than a continuous ascending.Correlation analysis indicates that this phenomenon is attributed to the introduction of Cr,which promotes the formation of FeCr_(2)O_(4) in the rust layer.FeCr_(2)O_(4) helps prevent chloride ions from penetrating the rust layer,exerting a protective effect.These findings provide a strong scientific foundation for the design and improvement of new high-Cr-content weathering bridge steels.展开更多
Aegilops variabilis(S^(v)S^(v)U^(v)U^(v))is a source of resistance to wheat stripe rust.The phKL locus in Chinese common wheat landrace Kaixian-Luohanmai(KL)can induce homoeologous wheat-alien chromosome pairing and r...Aegilops variabilis(S^(v)S^(v)U^(v)U^(v))is a source of resistance to wheat stripe rust.The phKL locus in Chinese common wheat landrace Kaixian-Luohanmai(KL)can induce homoeologous wheat-alien chromosome pairing and recombination.In this study,we confirmed that the whole 2S^(v)chromosome introgressed into wheat from Ae.variabilis accession AS116 conferred all-stage stripe rust resistance.The underlying gene(s),named YrAev,was mapped to the long arm 2S^(v)L using an F_(2)population.Two 2S^(v)-2B recombinants,derived from a cross of the 2S^(v)(2B)chromosome substitution line and KL,were confirmed to harbor the resistance locus.The physical region containing YrAev,determined from RNA-seq data,was 844.6-852.1 Mb on the chromosome arm 2S^(l)of the Ae.longissima(S^(v)genome donor species of Ae.variabilis)accession TL05 assembly v1.0.Differential gene expression analysis of post-inoculation with the Pst race has indicated two disease-resistance-related genes(annotated as mixed lineage kinase domain-like protein and nucleotide-binding leucine-rich repeat like protein,respectively)as promising candidates for YrAev.This study demonstrates the utility of the phKL gene system in alien gene localization and transfer.The resistant translocation line harboring YrAev can be exploited by wheat breeders as a novel source of resistance to stripe rust.展开更多
Southern corn rust(SCR)is an airborne fungal disease caused by Puccinia polysora Underw.(P.polysora)that adversely impacts maize quality and yields worldwide.Screening for new elite SCR-resistant maize loci or genes h...Southern corn rust(SCR)is an airborne fungal disease caused by Puccinia polysora Underw.(P.polysora)that adversely impacts maize quality and yields worldwide.Screening for new elite SCR-resistant maize loci or genes has the potential to enhance overall resistance to this pathogen.Using phenotypic SCR resistance-related data collected over two years and three geographical environments,a genome-wide association study was carried out in this work,which eventually identified 91 loci that were substantially correlated with SCR susceptibility.These included 13 loci that were significant in at least three environments and overlapped with 74 candidate genes(B73_RefGen_v4).Comparative transcriptomic analyses were then performed to identify the genes related to SCR infection,with 2,586 and 797 differentially expressed genes(DEGs)ultimately being identified in the resistant Qi319and susceptible 8112 inbred lines following P.polysora infection,respectively,including 306 genes common to both lines.Subsequent integrative multi-omics investigations identified four potential candidate SCR response-related genes.One of these genes is ZmHCT9,which encodes the protein hydroxycinnamoyl transferase 9.This gene was up-regulated in susceptible inbred lines and linked to greater P.polysora resistance as confirmed through cucumber mosaic virus(CMV)-based virus induced-gene silencing(VIGS)system-mediated gene silencing.These data provide important insights into the genetic basis of the maize SCR response.They will be useful for for future research on potential genes related to SCR resistance in maize.展开更多
Dibromoethane is a widespread,persistent organic pollutant.Biochars are known mediators of reductive dehalogenation by layered Fe^(Ⅱ)-Fe^(Ⅲ)hydroxides(green rust),which can reduce 1,2-dibromoethane to innocuous brom...Dibromoethane is a widespread,persistent organic pollutant.Biochars are known mediators of reductive dehalogenation by layered Fe^(Ⅱ)-Fe^(Ⅲ)hydroxides(green rust),which can reduce 1,2-dibromoethane to innocuous bromide and ethylene.However,the critical characteristics that determine mediator functionality are lesser known.Fifteen biochar substrates were pyrolyzed at 600℃and 800℃,characterized by elemental analysis,X-ray photo spectrometry C and N surface speciation,X-ray powder diffraction,specific surface area analysis,and tested for mediation of reductive debromination of 1,2-dibromoethane by a green rust reductant under anoxic conditions.A statistical analysis was performed to determine the biochar properties,critical for debromination kinetics and total debromination extent.It was shown that selected plant based biochars can mediate debromination of 1,2-dibromoethane,that the highest first order rate constant was 0.082/hr,and the highest debromination extent was 27%in reactivity experiments with 0.1μmol(20μmol/L)1,2-dibromoethane,≈22 mmol/L Fe^(Ⅱ)GR,and 0.12 g/L soybean meal biochar(7 days).Contents of Ni,Zn,N,and P,and the relative contribution of quinone surface functional groups were significantly(p<0.05)positively correlated with 1,2-dibromoethane debromination,while adsorption,specific surface area,and the relative contribution of pyridinic N oxide surface groups were significantly negatively correlated with debromination.展开更多
Yellow rust(Puccinia striiformis f.sp.Tritici,YR)and fusarium head blight(Fusarium graminearum,FHB)are the two main diseases affecting wheat in the main grain-producing areas of East China,which is common for the two ...Yellow rust(Puccinia striiformis f.sp.Tritici,YR)and fusarium head blight(Fusarium graminearum,FHB)are the two main diseases affecting wheat in the main grain-producing areas of East China,which is common for the two diseases to appear simultaneously in some main production areas.It is necessary to discriminate wheat YR and FHB at the regional scale to accurately locate the disease in space,conduct detailed disease severity monitoring,and scientific control.Four images on different dates were acquired from Sentinel-2,Landsat-8,and Gaofen-1 during the critical period of winter wheat,and 22 remote sensing features that characterize the wheat growth status were then calculated.Meanwhile,6 meteorological parameters that reflect the wheat phenological information were also obtained by combining the site meteorological data and spatial interpolation technology.Then,the principal components(PCs)of comprehensive remote sensing and meteorological features were extracted with principal component analysis(PCA).The PCs-based discrimination models were established to map YR and FHB damage using the random forest(RF)and backpropagation neural network(BPNN).The models’performance was verified based on the disease field truth data(57 plots during the filling period)and 5-fold cross-validation.The results revealed that the PCs obtained after PCA dimensionality reduction outperformed the initial features(IFs)from remote sensing and meteorology in discriminating between the two diseases.Compared to the IFs,the average area under the curve for both micro-average and macro-average ROC curves increased by 0.07 in the PCs-based RF models and increased by 0.16 and 0.13,respectively,in the PCs-based BPNN models.Notably,the PCs-based BPNN discrimination model emerged as the most effective,achieving an overall accuracy of 83.9%.Our proposed discrimination model for wheat YR and FHB,coupled with multi-source remote sensing images and meteorological data,overcomes the limitations of a single-sensor and single-phase remote sensing information in multiple stress discrimination in cloudy and rainy areas.It performs well in revealing the damage spatial distribution of the two diseases at a regional scale,providing a basis for detailed disease severity monitoring,and scientific prevention and control.展开更多
The intensification of global warming has led to the continuous outbreak of southern rust(Puccinia polysora Underw.)in major maize-producing regions worldwide.The severe outbreak in the Huang-Huai-Hai summer maize reg...The intensification of global warming has led to the continuous outbreak of southern rust(Puccinia polysora Underw.)in major maize-producing regions worldwide.The severe outbreak in the Huang-Huai-Hai summer maize region of China in 2021 caused yield losses exceeding 50%in some plots,and this disease has been included in the List of Key Crop Pests and Diseases.This paper systematically reviews the molecular resistance mechanisms of maize to southern rust,focusing on the immune mechanisms mediated by NLR family genes and the characteristics of the Bin 10.01 resistance gene cluster;it summarizes the advances in research of molecular breeding technologies such as gene marker development,map-based cloning,and gene editing;combined with the disease characteristics of the spring-sown maize region in Southwest China and the summer-sown maize region in Huang-Huai-Hai,it elaborates on regionally adapted prevention and control strategies;integrating breeding practices of Dunhuang Seed Industry Group(e.g.,Dunyu 810 and Dunyan 616),it proposes a full-chain solution of"precision gene pyramiding-heterotic group utilization-regional promotion".It is expected to provide theoretical and technical references for molecular breeding of maize resistance to southern rust.展开更多
Wheat stripe rust,a devastating disease caused by the fungal pathogen Puccinia striiformis f.sp.tritici(Pst),poses a significant threat to global wheat production.Growing resistant cultivars is a crucial strategy for ...Wheat stripe rust,a devastating disease caused by the fungal pathogen Puccinia striiformis f.sp.tritici(Pst),poses a significant threat to global wheat production.Growing resistant cultivars is a crucial strategy for wheat stripe rust management.However,the underlying molecular mechanisms of wheat resistance to Pst remain incompletely understood.To unravel these mechanisms,we employed high-throughput RNA sequencing(RNA-Seq)to analyze the transcriptome of the resistant wheat cultivar Mianmai 46(MM46)at different time points(24,48,and 96 h)post-inoculation with the Pst race CYR33.The analysis revealed that Pst infection significantly altered the expression of genes involved in photosynthesis and energy metabolism,suggesting a disruption of host cellular processes.Conversely,the expression of several resistance genes was upregulated,indicating activation of defense responses.Further analysis identified transcription factors(TFs),pathogen-related(PR)proteins,and chitinase-encoding genes as key players in wheat resistance to Pst.These genes likely contribute to the activation of defense pathways,such as the oxidative burst,which involves the production of reactive oxygen species(ROS).The activities of antioxidant enzymes,including peroxidase(POD),superoxide dismutase(SOD),and catalase(CAT),were also upregulated,suggesting a role in mitigating oxidative damage caused by ROS.Our findings provide valuable insights into the molecular mechanisms underlying wheat resistance to Pst.By identifying key genes and pathways involved in this complex interaction,we can develop more effective strategies for breeding resistant wheat cultivars and managing this destructive disease.展开更多
Barley(Hordeum vulgare L.)is a significant global crop that thrives in various climatic and drought-stress conditions.Furthermore,increased drought intervals and more significant weather variability resulting from cli...Barley(Hordeum vulgare L.)is a significant global crop that thrives in various climatic and drought-stress conditions.Furthermore,increased drought intervals and more significant weather variability resulting from climate change can affect the severity of plant diseases.Therefore,two primary objectives of integrated disease management regarding climate change are identifying cultivars resistant to foliar diseases and understanding disease progression under abiotic stress.In the current study,we assessed the quantitative foliar disease resistance of 17 commercial barley cultivars under both normal and water stress conditions over two growing seasons(from 2020/21 to 2021/22).The findings demonstrated a reduced incidence of foliar fungal diseases(leaf rust,net blotch,and powdery mildew)under severe drought stress relative to standard irrigated field conditions.The barley cultivars(Giza 130,Giza 131,and Giza 133)demonstrated significant differences across all disease resistance indices.In addition,the study aimed to molecularly characterize 17 commercial barley varieties using single-cell DNA testing(SCoT)to identify genetic polymorphism and specific markers for each genotype.Eight SCoT primers were employed to investigate the genetic polymorphism among 17 barley varieties.Furthermore,these cultivars exhibited optimal performance for the majority of agricultural attributes examined,both under normal and water-stressed conditions.展开更多
基金Item Sponsored by High Technology Research and Development Program(863Program) of China(2007AA03Z504)
文摘The corrosion behavior of a rusted 550 MPa grade offshore platform steel in Clcontaining environment was investigated.The results revealed that the corrosion process can be divided into initial stage in which corrosion rate increased with accumulation of corrosion products and later stage in which homogeneous and compact rust layer started to protect steel substrate out of corrosion mediums.On the contrary,structural analysis of rust layers by X-ray diffraction showed that α-FeOOH increased from 1.3% to 3.6% and the Fe3O4 increased from 1.0% to 1.5% while γ-FeOOH reduced slightly according to corrosion time increased from 30 cycles to 73 cycles.The results of electron probe microanalysis indicated that Cr concentrated mainly in the inner region of the rust,inner/outer interface especially,whereas Ni and Cu were uniformly distributed all over the rust after 73 corrosion cycles.According to electrochemical measurements,it was found that the corrosion rate of rusted steel reduced from 0.61 mm/a after 45 cycles to 0.34 mm/a after 85 cycles,44.3% reduction approximately,and Rrust values increased with increment of corrosion time.Therefore,formation of compact inner rust layer and enrichment of Cr are important to improve corrosion resistance of offshore platform steel.
基金the National Natural Science Foundation of China(Project No.51571027)for funding support
文摘The relationship between the specific surface area（SSA） of rust and the electrochemical behavior of rusted steel under wet-dry acid corrosion conditions was investigated. The results showed that the corrosion current density first increased and then decreased with increasing SSA of the rust during the corrosion process. The structure of the rust changed from single-layer to double-layer, and the γ-FeOOH content decreased in the inner layer of the rust with increasing corrosion time; by contrast, the γ-FeOOH content in the outer layer was constant. When the SSA of the rust was lower than the critical SSA corresponding to the relative humidity during the drying period, condensed water in the micropores of the rust could evaporate, which prompted the diffusion of O_2 into the rust and the following formation process of γ-FeOOH, leading to an increase of corrosion current density with increasing corrosion time. However, when the SSA of the rust reached or exceeded the critical SSA, condensate water in the micro-pores of the inner layer of the rust could not evaporate which inhibited the diffusion of O_2 and decreased the γ-FeOOH content in the inner rust, leading to a decrease of corrosion current density with increasing corrosion time.
文摘The biotrophic fungus Puccinia striiformis f. sp. tritici is the causal agent of the yellow rust in wheat. Between the years 2010–2013 a new strain of this pathogen(Warrior/Ambition),against which the present cultivated wheat varieties have no resistance, appeared and spread rapidly. It threatens cereal production in most of Europe. The search for sources of resistance to this strain is proposed as the most efficient and safe solution to ensure high grain production. This will be helped by the development of high performance and low cost techniques for field phenotyping. In this study we analyzed vegetation indices in the Red,Green, Blue(RGB) images of crop canopies under field conditions. We evaluated their accuracy in predicting grain yield and assessing disease severity in comparison to other field measurements including the Normalized Difference Vegetation Index(NDVI), leaf chlorophyll content, stomatal conductance, and canopy temperature. We also discuss yield components and agronomic parameters in relation to grain yield and disease severity.RGB-based indices proved to be accurate predictors of grain yield and grain yield losses associated with yellow rust(R2= 0.581 and R2= 0.536, respectively), far surpassing the predictive ability of NDVI(R2= 0.118 and R2= 0.128, respectively). In comparison to potential yield, we found the presence of disease to be correlated with reductions in the number of grains per spike, grains per square meter, kernel weight and harvest index. Grain yield losses in the presence of yellow rust were also greater in later heading varieties. The combination of RGB-based indices and days to heading together explained 70.9% of the variability in grain yield and 62.7% of the yield losses.
基金financially supported by the National Natural Science Foundation of China (Nos. 51001108 and 51131007)the National Basic Research Program of China (No. 2010CB934604)
文摘An eco-friendly rust converter treatment based on tannic acid was optimized by orthogonal experiment, and the adhesion of the epoxy top-coating system was evaluated. The results showed that the adhesion between the epoxy coating and the rusted steel after treatment was enhanced from 1.93 to 5.97 MPa. This adhesion improvement was attributed to the formation of the micro-cracked tannic conversion layer and the increase of the compactness of the rust layer. The failure analysis suggested that the remainder rust was still the key issue for the further adhesion improvement of the coating system.
基金funded by the Key R&D Program of Yangling Seed Industry Innovation,China(Ylzy-xm-02)the Young Elite Scientists Sponsorship Program by China Association for Science and Technology(2021QNRC001)。
文摘Thinopyrum ponticum(2n=10×=70),a wild relative of common wheat(Triticum aestivum L.),is considered an invaluable genetic resource for wheat improvement due to its abundance of genes conferring resistance to biotic and abiotic stresses.This study focused on the CH97 line,derived from the BC1F7 progeny of a cross between wheat cv.7182 and Th.ponticum.Cytological evidence showed that CH97 has 42 chromosomes,forming 21 bivalents at meiotic metaphase I,with the bivalents subsequently separating and moving to opposite poles during meiotic anaphase I.Through a combination of fluorescence in situ hybridization(FISH),genomic in situ hybridization(GISH),multicolor GISH(mc-GISH),and liquid array analysis,it was determined that CH97 comprises 40 wheat chromosomes and two alien chromosomes from the Ee genome of Th.ponticum,featuring the absence of a pair of 5D chromosomes and variations in 1B,6B,and 7B chromosomes.These findings confirm that CH97 is a stable wheat-Th.ponticum 5E(5D)alien disomic substitution line.Inoculation experiments revealed that CH97 exhibits high resistance to wheat powdery mildew and stripe rust throughout the growth period,in contrast to the highly susceptible common wheat parent 7182.Compared to 7182,CH97 displayed improvements in thousand-kernel weight and kernel length.Additionally,utilizing specific-locus amplified fragment sequencing(SLAF-seq)technology,chromosome 5E-specific molecular markers were developed and validated,achieving a 33.3% success rate,facilitating marker-assisted selection for disease resistance in wheat.Overall,the CH97 substitution line,with its resistance to diseases and improved agronomic traits,represents valuable new germplasm for wheat chromosome engineering and breeding.
基金financially supported by Xinjiang Major Science and Technology Projects,China (2023A02009)。
文摘In China,Xinjiang is a relatively independent epidemic region of wheat stripe rust,caused by Puccinia striiformis f.sp.tritici,due to great population genetic divergence of Xinjiang with other inland epidemic regions.In this region,race evolution was usually slower than inland populations.However,many new races have recently been found,and therefore,it is necessary for more understanding of the virulence evolution of the Xinjiang population.So,in this study,a 65 sexual progenies,derived from a Xinjiang single-urediospore isolate BGTB-1 of P.striiformis f.sp.tritici by selfing on alternate host barberry(Berberis aggregata).It was phenotyped on the 25 single Yr lines,and genotyped by 19 kompetitive allele-specific PCRsingle nucleotide polymorphism(KASP-SNP) markers.As a result,the 65 progenies were identified as 56 various virulence patterns(VPs),and neither of which was identical to the parental isolate,showed 100% virulence variation.Compared with the parental isolate,of all progenies,39(60.0%) had increased virulence,and 18(27.7%) had decreased virulence.All progenies exhibited avirulence to Yr10,Yr15,Yr32,and YrTr1 loci,and avirulence and virulence segregation at the remaining 21 Yr resistance loci.The results showed avirulence to Yr5,Yr7,and Yr76(A:V≈3:1) loci is controlled by a single dominant gene,and that to Yr6,Yr25,and Yr44(A:V≈1:3) loci by a single recessive gene.However,avirulence to the remaining 15 resistant loci including Yr1,Yr2,Yr3,Yr4,Yr8,Yr9,Yr17,Yr26(=Yr24),Yr28,Yr29,Yr43,YrSP,Yr27,YrA,and YrExp2,with various avirulence and virulence segregation ratios,is controlled by two genes with different gene effects,indicating complex genetic traits of the parental isolate.Totally,65 dissimilar genotypes detected among progenies using overall molecular markers,by which a linkage map was constructed,with a genetic distance of 441.0 cM.Interestingly,although the parental isolate was avirulent to Yr5,but 17 progenies showed virulence,showing the change of pathogenicity from avirulence to virulence at this resistance locus.It was for the first time to report that progenies with virulence to Yr5 produced sexually from avirulent parental isolate at this resistance locus.To our knowledge,this study offers an insight into inheritance,sexual reproduction and virulence variation of P.striiformis f.sp.tritici in Xinjiang,facilitating us to understand evolution of the rust pathogen in this region and accounting for Xinjiang population distinguished form other inland populations.Additionally,it is necessary to further confirm the roles of sexual reproduction in the emergence of new races and affecting population genetic diversity of P.striiformis f.sp.tritici under natural conditions in Xinjiang.
基金Project(52373065)supported by the National Natural Science Foundation of ChinaProject(2220004002898)supported by the Zhuhai Industry University Research Cooperation and Basic and Applied Research Projects,China。
文摘Waterborne acrylic coatings are widely utilized due to their cost-effectiveness,high transparency,strong resistance to weather and chemicals,impressive mechanical properties,and excellent adhesion to various substrates.In these coatings,a reactive emulsifier containing phosphate groups can be integrated into the molecular chain during polymerization,which enhances the coating's compactness and corrosion resistance.This work focuses on the synthesis of styrene-butyl acrylate(St-BA)latex and methyl methacrylate-butyl acrylate(MMA-BA)latex using the reactive phosphate emulsifier ANPEO_(10)-P_(1) through seed emulsion polymerization,achieving a conversion rate of approximately 99%and a solid content close to 50%.The resulting coatings from St-BA and MMA-BA latexes demonstrated long-term corrosion protection for carbon steel and aluminum alloy due to in-situ phosphatization,effectively preventing flash rust.Notably,the MMA-BA coating exhibited remarkable durability,enduring immersion for up to 1224 h(51 d)on Q 235 carbon steel before reaching the failure threshold(|Z|0.01 Hz£106Ω·cm^(2))on Q 235 carbon steel.On 5052 aluminum alloy,the St-BA coating maintained|Z|0.01 Hz>10^(8)Ω·cm^(2) for 480 h(20 d).Furthermore,the corrosion resistance of St-BA and MMA BA coatings on Q 235 steel sheet and 5052 aluminum alloy surpassed that of commercially available MMA-BA and St BA coatings after immersion in a 3.5 wt%NaCl aqueous solution.This work also delves into the anticorrosion mechanism of MMA-BA and St-BA coatings.
基金supported by the Fundamental Research Program of Shanxi Province(Grant Nos.202203021222188 and 202303021211166)Scientific Research Fund of Taiyuan University of Science and Technology(Grant No.20222073)+1 种基金Award Fund for Outstanding Doctors in Shanxi Province(Grant No.20232047)Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(Grant No.2022L280).
文摘The crevice corrosion of the reinforcing steel in the carbonated simulated concrete pore solutions(SCPSs)containing 0.04 mol/L chloride was investigated.In comparison,the steel without crevice covered on its surface was also studied.Results showed that the crevice corrosion in the uncarbonated SCPS was not triggered and the steel remained passivity by suppressing the anodic dissolution in the crevice interior.As SCPS was carbonated to have pH values of 10.5 and 11.5,the crevice corrosion was easier to be activated than the widely reported pitting corrosion owing to the rapid oxygen depletion within the crevice,but the crevice corrosion damage might be alleviated to a certain extent due to the relative compact surface film formed inside the crevice.The further carbonation led to the activation dissolution of both the crevice interior and exterior,causing the more serious corrosion damage.Corrosion process of the crevice corrosion was systematically analyzed by combining the electrochemical measurement results and Evens polarization diagram.
基金supported by the National Natural Science Foundation of China(No.52171063).
文摘The rust layer is a critical factor in determining the corrosion resistance performance of weathering bridge steel.Understanding the evolution mechanism of this rust layer is fundamental for the design and optimization of such steel.This study investigates the evolu-tion of the rust layer on high-Cr-content weathering bridge steel,using an atmospheric corrosion monitoring(ACM)sensor and big data mining techniques in a simulated tropical marine atmosphere.Results reveal that the protective properties of the rust layer follow a peri-odic pattern of“ascending–constant”rather than a continuous ascending.Correlation analysis indicates that this phenomenon is attributed to the introduction of Cr,which promotes the formation of FeCr_(2)O_(4) in the rust layer.FeCr_(2)O_(4) helps prevent chloride ions from penetrating the rust layer,exerting a protective effect.These findings provide a strong scientific foundation for the design and improvement of new high-Cr-content weathering bridge steels.
基金supported by the National Natural Science Foundation of China(32172020,31971884)the National Key Research and Development Program of China(2024YFD1201202,2024YFD1200402)+4 种基金the Sichuan Science and Technology Program(2022ZDZX0014,2023YFN0085)the Qinghai Provincial Key Laboratory of Crop Molecular Breeding(2023-1-1)the State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China(SKLKF202409)the Alliance of National and International Science Organizations for the Belt and Road Regions(ANSO-CR-KP-202205)the International Partnership Program of Chinese Academy of Sciences(077GJHZ2023028GC)。
文摘Aegilops variabilis(S^(v)S^(v)U^(v)U^(v))is a source of resistance to wheat stripe rust.The phKL locus in Chinese common wheat landrace Kaixian-Luohanmai(KL)can induce homoeologous wheat-alien chromosome pairing and recombination.In this study,we confirmed that the whole 2S^(v)chromosome introgressed into wheat from Ae.variabilis accession AS116 conferred all-stage stripe rust resistance.The underlying gene(s),named YrAev,was mapped to the long arm 2S^(v)L using an F_(2)population.Two 2S^(v)-2B recombinants,derived from a cross of the 2S^(v)(2B)chromosome substitution line and KL,were confirmed to harbor the resistance locus.The physical region containing YrAev,determined from RNA-seq data,was 844.6-852.1 Mb on the chromosome arm 2S^(l)of the Ae.longissima(S^(v)genome donor species of Ae.variabilis)accession TL05 assembly v1.0.Differential gene expression analysis of post-inoculation with the Pst race has indicated two disease-resistance-related genes(annotated as mixed lineage kinase domain-like protein and nucleotide-binding leucine-rich repeat like protein,respectively)as promising candidates for YrAev.This study demonstrates the utility of the phKL gene system in alien gene localization and transfer.The resistant translocation line harboring YrAev can be exploited by wheat breeders as a novel source of resistance to stripe rust.
基金supported by the National Key R&D Program of China(2022YFD1201802)the Shandong Key R&D Program,China(2022CXGC010607)+2 种基金the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202109)the Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP2017-ICS)the Subproject of the Major Project of Science and Technology in Shanxi Province,China(202201140601025-1-02)。
文摘Southern corn rust(SCR)is an airborne fungal disease caused by Puccinia polysora Underw.(P.polysora)that adversely impacts maize quality and yields worldwide.Screening for new elite SCR-resistant maize loci or genes has the potential to enhance overall resistance to this pathogen.Using phenotypic SCR resistance-related data collected over two years and three geographical environments,a genome-wide association study was carried out in this work,which eventually identified 91 loci that were substantially correlated with SCR susceptibility.These included 13 loci that were significant in at least three environments and overlapped with 74 candidate genes(B73_RefGen_v4).Comparative transcriptomic analyses were then performed to identify the genes related to SCR infection,with 2,586 and 797 differentially expressed genes(DEGs)ultimately being identified in the resistant Qi319and susceptible 8112 inbred lines following P.polysora infection,respectively,including 306 genes common to both lines.Subsequent integrative multi-omics investigations identified four potential candidate SCR response-related genes.One of these genes is ZmHCT9,which encodes the protein hydroxycinnamoyl transferase 9.This gene was up-regulated in susceptible inbred lines and linked to greater P.polysora resistance as confirmed through cucumber mosaic virus(CMV)-based virus induced-gene silencing(VIGS)system-mediated gene silencing.These data provide important insights into the genetic basis of the maize SCR response.They will be useful for for future research on potential genes related to SCR resistance in maize.
文摘Dibromoethane is a widespread,persistent organic pollutant.Biochars are known mediators of reductive dehalogenation by layered Fe^(Ⅱ)-Fe^(Ⅲ)hydroxides(green rust),which can reduce 1,2-dibromoethane to innocuous bromide and ethylene.However,the critical characteristics that determine mediator functionality are lesser known.Fifteen biochar substrates were pyrolyzed at 600℃and 800℃,characterized by elemental analysis,X-ray photo spectrometry C and N surface speciation,X-ray powder diffraction,specific surface area analysis,and tested for mediation of reductive debromination of 1,2-dibromoethane by a green rust reductant under anoxic conditions.A statistical analysis was performed to determine the biochar properties,critical for debromination kinetics and total debromination extent.It was shown that selected plant based biochars can mediate debromination of 1,2-dibromoethane,that the highest first order rate constant was 0.082/hr,and the highest debromination extent was 27%in reactivity experiments with 0.1μmol(20μmol/L)1,2-dibromoethane,≈22 mmol/L Fe^(Ⅱ)GR,and 0.12 g/L soybean meal biochar(7 days).Contents of Ni,Zn,N,and P,and the relative contribution of quinone surface functional groups were significantly(p<0.05)positively correlated with 1,2-dibromoethane debromination,while adsorption,specific surface area,and the relative contribution of pyridinic N oxide surface groups were significantly negatively correlated with debromination.
基金supported by National Key R&D Program of China(2022YFD2000100)National Natural Science Foundation of China(42401400)Zhejiang Provincial Key Research and Development Program(2023C02018).
文摘Yellow rust(Puccinia striiformis f.sp.Tritici,YR)and fusarium head blight(Fusarium graminearum,FHB)are the two main diseases affecting wheat in the main grain-producing areas of East China,which is common for the two diseases to appear simultaneously in some main production areas.It is necessary to discriminate wheat YR and FHB at the regional scale to accurately locate the disease in space,conduct detailed disease severity monitoring,and scientific control.Four images on different dates were acquired from Sentinel-2,Landsat-8,and Gaofen-1 during the critical period of winter wheat,and 22 remote sensing features that characterize the wheat growth status were then calculated.Meanwhile,6 meteorological parameters that reflect the wheat phenological information were also obtained by combining the site meteorological data and spatial interpolation technology.Then,the principal components(PCs)of comprehensive remote sensing and meteorological features were extracted with principal component analysis(PCA).The PCs-based discrimination models were established to map YR and FHB damage using the random forest(RF)and backpropagation neural network(BPNN).The models’performance was verified based on the disease field truth data(57 plots during the filling period)and 5-fold cross-validation.The results revealed that the PCs obtained after PCA dimensionality reduction outperformed the initial features(IFs)from remote sensing and meteorology in discriminating between the two diseases.Compared to the IFs,the average area under the curve for both micro-average and macro-average ROC curves increased by 0.07 in the PCs-based RF models and increased by 0.16 and 0.13,respectively,in the PCs-based BPNN models.Notably,the PCs-based BPNN discrimination model emerged as the most effective,achieving an overall accuracy of 83.9%.Our proposed discrimination model for wheat YR and FHB,coupled with multi-source remote sensing images and meteorological data,overcomes the limitations of a single-sensor and single-phase remote sensing information in multiple stress discrimination in cloudy and rainy areas.It performs well in revealing the damage spatial distribution of the two diseases at a regional scale,providing a basis for detailed disease severity monitoring,and scientific prevention and control.
基金Supported by Central Government Funds for Guiding Local Scientific and Technological Development(24ZYQF002)Major Science and Technology Project of Gansu Province(24ZDNF001)+1 种基金National Key R&D Program"Exploration and Utilization of Disease-and Pest-Resistant and High-Yield Gene Resources in Maize"(2022YDF1201800)Key Laboratory of Mechanized Maize Variety Creation,Ministry of Agriculture and Rural Affairs.
文摘The intensification of global warming has led to the continuous outbreak of southern rust(Puccinia polysora Underw.)in major maize-producing regions worldwide.The severe outbreak in the Huang-Huai-Hai summer maize region of China in 2021 caused yield losses exceeding 50%in some plots,and this disease has been included in the List of Key Crop Pests and Diseases.This paper systematically reviews the molecular resistance mechanisms of maize to southern rust,focusing on the immune mechanisms mediated by NLR family genes and the characteristics of the Bin 10.01 resistance gene cluster;it summarizes the advances in research of molecular breeding technologies such as gene marker development,map-based cloning,and gene editing;combined with the disease characteristics of the spring-sown maize region in Southwest China and the summer-sown maize region in Huang-Huai-Hai,it elaborates on regionally adapted prevention and control strategies;integrating breeding practices of Dunhuang Seed Industry Group(e.g.,Dunyu 810 and Dunyan 616),it proposes a full-chain solution of"precision gene pyramiding-heterotic group utilization-regional promotion".It is expected to provide theoretical and technical references for molecular breeding of maize resistance to southern rust.
基金funded by the National Natural Science Foundation of China(32502517)the Open Project Program of State Key Laboratory of Crop Stress Biology for Arid Areas(SKLCSRHPKF20)+1 种基金Collaborative Innovation Project of Department of Education of Anhui Provincial(GXXT-2019-033)Horizontal project-Breeding of high yield and multi resistant wheat varieties(2021122401).
文摘Wheat stripe rust,a devastating disease caused by the fungal pathogen Puccinia striiformis f.sp.tritici(Pst),poses a significant threat to global wheat production.Growing resistant cultivars is a crucial strategy for wheat stripe rust management.However,the underlying molecular mechanisms of wheat resistance to Pst remain incompletely understood.To unravel these mechanisms,we employed high-throughput RNA sequencing(RNA-Seq)to analyze the transcriptome of the resistant wheat cultivar Mianmai 46(MM46)at different time points(24,48,and 96 h)post-inoculation with the Pst race CYR33.The analysis revealed that Pst infection significantly altered the expression of genes involved in photosynthesis and energy metabolism,suggesting a disruption of host cellular processes.Conversely,the expression of several resistance genes was upregulated,indicating activation of defense responses.Further analysis identified transcription factors(TFs),pathogen-related(PR)proteins,and chitinase-encoding genes as key players in wheat resistance to Pst.These genes likely contribute to the activation of defense pathways,such as the oxidative burst,which involves the production of reactive oxygen species(ROS).The activities of antioxidant enzymes,including peroxidase(POD),superoxide dismutase(SOD),and catalase(CAT),were also upregulated,suggesting a role in mitigating oxidative damage caused by ROS.Our findings provide valuable insights into the molecular mechanisms underlying wheat resistance to Pst.By identifying key genes and pathways involved in this complex interaction,we can develop more effective strategies for breeding resistant wheat cultivars and managing this destructive disease.
基金The authors express their appreciation to the Deanship of Research and Graduate Studies at King Khalid University for funding this work under grant number RGP2/233/45the support provided by the Deanship of Scientific Research,Vice Presidency for Graduate Studies and Scientific Research at King FaisalUniversity,Saudi Arabia,under Project No.KFU242905funding from Princess Nourah bint Abdulrahman University through the Research Supporting Project Number PNURSP2025R241 in Riyadh,Saudi Arabia.
文摘Barley(Hordeum vulgare L.)is a significant global crop that thrives in various climatic and drought-stress conditions.Furthermore,increased drought intervals and more significant weather variability resulting from climate change can affect the severity of plant diseases.Therefore,two primary objectives of integrated disease management regarding climate change are identifying cultivars resistant to foliar diseases and understanding disease progression under abiotic stress.In the current study,we assessed the quantitative foliar disease resistance of 17 commercial barley cultivars under both normal and water stress conditions over two growing seasons(from 2020/21 to 2021/22).The findings demonstrated a reduced incidence of foliar fungal diseases(leaf rust,net blotch,and powdery mildew)under severe drought stress relative to standard irrigated field conditions.The barley cultivars(Giza 130,Giza 131,and Giza 133)demonstrated significant differences across all disease resistance indices.In addition,the study aimed to molecularly characterize 17 commercial barley varieties using single-cell DNA testing(SCoT)to identify genetic polymorphism and specific markers for each genotype.Eight SCoT primers were employed to investigate the genetic polymorphism among 17 barley varieties.Furthermore,these cultivars exhibited optimal performance for the majority of agricultural attributes examined,both under normal and water-stressed conditions.
