Objective:To explore the accuracy and stability of the results of peripheral blood routine tests at different time points after anticoagulation and standing,providing a scientific basis for actual clinical work.Method...Objective:To explore the accuracy and stability of the results of peripheral blood routine tests at different time points after anticoagulation and standing,providing a scientific basis for actual clinical work.Methods:In this study,30 patients who visited the hospital in October 2023 were randomly selected and divided into two groups(15 cases in each group).The same collection method was used for routine blood tests.The tests were performed after anticoagulation and standing for 5 minutes,1 hour,and 5 minutes,2 hours respectively,and the routine blood test indicators at different time points were compared.Results:After comparison,there were no significant differences in the results of routine blood tests at 5 minutes after mixing,anticoagulating,and standing peripheral blood and those at 1 hour and 2 hours(P>0.05).Conclusion:The results of peripheral blood after mixing,anticoagulating,and standing for 5 minutes are stable compared with those after standing for 1 hour and 2 hours.In actual work,the pre-test turnaround time can be appropriately extended.展开更多
OBJECTIVE:To reach consensus on the diagnostic criteria of syndrome of dampness obstruction in idiopathic membranous nephropathy(IMN)patients by literature research and expert investigation(interviews and a Delphi met...OBJECTIVE:To reach consensus on the diagnostic criteria of syndrome of dampness obstruction in idiopathic membranous nephropathy(IMN)patients by literature research and expert investigation(interviews and a Delphi method).METHODS:Our study was consistent with T/CACM 1336-2020.We searched the monographs and references published in the past 40 years(1983-2022),and established the diagnostic criteria pool of waterdampness syndrome and dampness-turbidity syndrome in Traditional Chinese Medicine(TCM)based on literature by using frequency statistics and correlation analysis.Expert investigation(interview method and two rounds of Delphi method)was used to form the diagnostic criteria of water-dampness syndrome and dampnessturbidity syndrome of idiopathic membranous nephropathy.Clinical diagnostic test research was carried out,and compared with“Diagnostic Criteria for dampness syndrome”(T/CACM 1454-2023)to evaluate the authenticity,reliability and clinical application value of the standard.RESULTS:A total of 122 relevant guides,standards,monographs and documents were included through searching books and Chinese databases.Four experts were interviewed and two rounds of delphi method(75 experts nationwide)were carried out.The experts'opinions are relatively concentrated and the differences are small.Based on the weight of each index,the diagnostic criteria indexes of water-dampness syndrome and dampness-turbidity syndrome were selected.After discussion by the core group members,the diagnostic model of"necessary symptoms and optional symptoms"was established,and the final diagnostic criteria of waterdampness syndrome and dampness-turbidity syndrome were established.One hundred and ninety-one inpatients and outpatients of Guangdong Provincial Hospital of Chinese Medicine from January 2021 to February 2023 were included in Diagnostic test study.There was no statistical difference in gender,age and course of disease(P>0.05).The sensitivity and specificity of the trial standard were 90.34%and 73.33%respectively,while the sensitivity and specificity of T/CACM 1454-2023 were 99.43%and 6.67%,respectively.CONCLUSIONS:The consensus-based diagnostic criteria for IMN can be widely incorporated in TCM.A further clinical study will be conducted to analyze the diagnosis value and cut-off score of our IMN criteria.展开更多
Urinalysis is one of the most widely developed clinical tests, which plays a key role in the treatment of various diseases of the urinary system, kidney system and system. Total urine test has the advantages of being ...Urinalysis is one of the most widely developed clinical tests, which plays a key role in the treatment of various diseases of the urinary system, kidney system and system. Total urine test has the advantages of being easy to use and quick to detect. However, in actual clinical trials, a number of factors, including the timing and quality of urine sample collection, the performance of testing equipment and operational standards, can affect the accuracy of the final test results. Therefore, understanding and mastering the influencing factors of clinical routine urine testing, adopting targeted response strategies in a timely manner, and reducing the influence of urine routine testing by external factors are the key to ensuring the accuracy of urine routine test results, and can also provide scientific and reliable basis for the diagnosis of clinical diseases. This paper further analyzes what are the common influencing factors in clinical urine routine testing, discusses related coping strategies, and how to better grasp and improve the quality of its examination in future work to minimize the impact.展开更多
By modifying the salicylic-acid moiety with electron-withdrawing or –donating groups, three new terbium complexes（LⅠ·Tb, LⅡ·Tb, L·（Ⅲ）Tb） based on tripodal carboxylate ligands were synthesized. D...By modifying the salicylic-acid moiety with electron-withdrawing or –donating groups, three new terbium complexes（LⅠ·Tb, LⅡ·Tb, L·（Ⅲ）Tb） based on tripodal carboxylate ligands were synthesized. Due to different pull-push electronic effects of ligands, the fluorescence intensities of these terbium complexes significantly varied, that is： LⅡ·Tb 〉L（Ⅲ）·Tb〉 LⅠ·Tb. Meanwhile, the characteristic peaks at 492 nm（5D4→7F6） and 547 nm（5 D4→7F5） showed ＂Off–On–Off＂ fluorescence response to various p H conditions,which indicated that all of them can be used as the highly sensitive pH fluorescent probes. Notably, using LⅡ·Tb with the best fluorescence performance as a probe, some patients＇ urine samples can be easily monitored through the response triggered by pH value. Therefore, LⅡ·Tb has the potential to auxiliarily diagnose some diseases in clinical practice through p H detection of routine urine test.展开更多
[Objectives]This study was conducted to analyze effects of antimicrobial peptides added to the diet of Tan sheep on their production, slaughter performance and blood composition. [Methods] Ninety two four-month-old Ta...[Objectives]This study was conducted to analyze effects of antimicrobial peptides added to the diet of Tan sheep on their production, slaughter performance and blood composition. [Methods] Ninety two four-month-old Tan sheep were randomly divided into two treatment groups according to their body weight, 46 in each group. The control check group(CK) was feed with conventional diet, and the experimental group was fed with the addition of antimicrobial peptide on the basis of the conventional diet, with the added amount of 2.5 g/sheep per day. The experimental period was 60 d. [Results] The incidence rate was 75.06% lower in the experimental group than in the CK. The average daily weight gain per sheep was 11.27% higher in the experimental group than in the CK(P<0.05). The feed conversion ratio was 8.45% lower in the experimental group than in the CK(P<0.05). The average daily gross profit per sheep was 12.12% higher in the experimental group than in the CK. For slaughter performance, the data difference of each item was not significant. The PH at 45 min and 24 h after slaughter was within the normal range of fresh mutton. The cooked meat percentage and water loss rate showed no significant differences(P>0.05). The marbling ranged from 2.45 to 2.50, indicating that the fat content was moderate, and the difference between groups was not significant(P>0.05). The flesh color ranged from 3.00 to 3.15, between light red and bright red, belonging to the normal color of mutton, and the difference between groups was not significant(P>0.05). The shear force was between 2.50 and 2.65, without a significant difference between groups(P>0.05). The white blood cells, lymphocytes and platelets in the experimental group were lower than those in the CK(P<0.01). The erythrocytes and hemoglobin in the erythrocyte group were higher than those in the CK(P<0.05). The neutrophils and monocytes in the experimental group were lower than those in the CK(P<0.05). [Conclusions] This study provides a technical basis for the rational use of antimicrobial peptides and their application in ruminants.展开更多
Objective: to explore the application of life test and routine test in the diagnosis of diabetes. Methods: 100 patients suspected of diabetic diseases were selected for biochemical test and routine test, and the resul...Objective: to explore the application of life test and routine test in the diagnosis of diabetes. Methods: 100 patients suspected of diabetic diseases were selected for biochemical test and routine test, and the results were compared. Results: compared with routine test, biochemical test has higher sensitivity, specificity and accuracy with significant difference (P<0.05). Conclusion: biochemical test has more clinical value in the diagnosis of diabetes.展开更多
To study and analyze the influence of peripheral blood and venous blood on the test results in blood routine test. Methods: from September 2020 to June 2021, 152 patients who underwent blood routine test were included...To study and analyze the influence of peripheral blood and venous blood on the test results in blood routine test. Methods: from September 2020 to June 2021, 152 patients who underwent blood routine test were included in the laboratory department of our hospital. All patients collected peripheral blood and venous blood for blood routine test respectively. The blood source of peripheral blood for blood routine test was peripheral blood group, and the blood source of elbow vein blood for blood routine test was venous blood group, The differences of blood routine test results between the two groups at 0 min were compared, and the blood sources of the two groups were tested for many times at 0 min, 10 min and 30 min after blood collection. The test results were analyzed and compared, and the satisfaction of the two blood collection methods, time and pain were evaluated. Results: at 0 min of blood collection, the leukocyte count value of venous blood group was lower than that of peripheral blood group, while the erythrocyte count value, hemoglobin concentration value, platelet count value, erythrocyte backlog value and average hemoglobin concentration value of venous blood group were higher than those of peripheral blood group (P < 0.05);However, there was no significant difference between the two groups (P > 0.05);The test results of peripheral blood group and venous blood group were compared for multiple tests at 0 min, 10 min and 30 min after blood collection respectively. There was no significant difference in the test results of erythrocyte count and hemoglobin concentration in peripheral blood group at each time period (P > 0.05), while the test results of leukocyte count and platelet count at 0 min were different from those at 10 min. There was significant difference in the results of 30 min test (P < 0.05), indicating that the index value will change with time;There was no significant difference in the test results of leukocyte count, erythrocyte count, hemoglobin concentration and platelet count in the venous blood group (P > 0.05), indicating that the indexes in the blood were relatively stable;Comparing the satisfaction scores of the two groups, the scores of the two groups were higher, and the satisfaction of peripheral blood was slightly higher, but there was no significant difference between the two groups (P > 0.05).Conclusion: comparing the satisfaction of the two groups of patients, the acceptability of the two blood collection methods is very high. From the analysis of the test results, on the whole, the test results of peripheral blood are slightly lower than those of venous blood. It is analyzed that the reason for this result may be that the peripheral blood causes the damage of blood cells during the extrusion process and reduces the count, and the white blood cell count. The platelet count value will change over time and affect the test results, while the venous blood test results have little difference within 30min, indicating that various indexes in venous blood are relatively stable within a certain period of time, and the accuracy of test results is higher.展开更多
Objective: the differences of blood routine test results between venous blood and capillary blood were analyzed. Methods: The subjects of the study were 120 healthy outpatients from August 2019 to August 2021. They we...Objective: the differences of blood routine test results between venous blood and capillary blood were analyzed. Methods: The subjects of the study were 120 healthy outpatients from August 2019 to August 2021. They were divided into two groups according to the random method (n=60 for each group). The capillary blood was collected in the control group and venous blood was collected in the observation group. The results of WBC, HCT, RBC, Hb, PLT, MCHC, MCV and HCT were statistically compared between the two groups. Results: WBC of the observation group was lower than that of the control group, HCT, RBC, Hb, PLT and MCHC of the observation group were higher than those of the control group, p < 0.05 (the difference was statistically significant). Compared with the control group, the MCV and HCT of the observation group had no significant difference (P>0.05). Conclusion: venous blood collection in blood routine test can improve the accuracy of test results, and can be the preferred collection method in blood collection with high clinical value.展开更多
Objective: to discuss the clinical value of new hemolysin and traditional hemolysin in blood routine examination. Methods: a total of 89 blood routine test subjects were selected from our hospital. The average control...Objective: to discuss the clinical value of new hemolysin and traditional hemolysin in blood routine examination. Methods: a total of 89 blood routine test subjects were selected from our hospital. The average control group (45 cases, using traditional hemolysin) and the study group (44 cases, using new hemolysin) were compared. Results: there were no statistically significant differences in the levels of red blood cells, white blood cells and platelets between the two groups (P > 0.05). The level of hemoglobin and satisfaction score in the study group were significantly higher than those in the control group (P < 0.05). The test time and cost in the study group were significantly lower than those in the control group (P < 0.05). Conclusion: the new hemolysin has more application value in routine blood test.展开更多
Objective: this study focuses on the main causes and solutions of the routine blood test. Methods: in this study, 120 patients with the error in the routine blood test results from March 2020 to March 2021 were random...Objective: this study focuses on the main causes and solutions of the routine blood test. Methods: in this study, 120 patients with the error in the routine blood test results from March 2020 to March 2021 were randomly selected as the study subjects, and the clinical data of all the study subjects were analyzed retrospectively. All patients received 2mL blood collection from peripheral blood and 14ml of venous blood. The venous blood samples were divided into 7 groups, among which group 1 was tested immediately after collection, groups 2 and 3 were stored in refrigerator (4℃) and room temperature, groups 4 and 4 hours respectively, group 6 and 7 were treated with 1.6 mg/ml and 2.8 mg/ml anticoagulant respectively. Peripheral blood needs to be tested immediately. The root causes of common errors are summarized, and the influence of different blood collection location, placement time and anticoagulant concentration on the routine blood test indicators is discussed, and the corresponding solutions are summarized. Results: the results of this study showed that platelet content in peripheral blood samples was significantly lower than venous blood, with significantly different data between groups (P <0.05). Blood samples were retained for 2 hours after collection, the hemoglobin concentration and platelet content were lower than the 4-hour retained samples, and the data differences between groups were significant (P <0.05).Blood samples were stored in the refrigerator, the hemoglobin concentration was lower than those stored at room temperature, and there were significant differences between groups (P <0.05).After the blood samples were treated with 2.8 mg/ml anticoagulant concentration, the content of each index was significantly higher than that treated with 1.6 mg/ml, and the data between the two groups varied significantly (P <0.05).Among the routine blood tests, 120 subjects had the error in the routine blood test indicators, including the unreasonable anticoagulant concentration, long inspection time, unreasonable storage and personal factors of the subjects. Conclusion: in the routine blood test, many factors resulted in the test error. The hospital should clarify the main influencing factors, improve the efficiency and quality of routine blood test by strengthening the training of relevant personnel, actively communicating with the subjects, and making reasonable use of anticoagulant, reducing the probability of error, and provide reliable support for the clinical diagnosis and treatment of doctors.展开更多
Objective: to analyze the diagnostic value of blood routine examination in children with bacterial infection. Methods: the clinical data of children with infectious diseases examined in our hospital were retrospective...Objective: to analyze the diagnostic value of blood routine examination in children with bacterial infection. Methods: the clinical data of children with infectious diseases examined in our hospital were retrospectively analyzed. From March 2019 to March 2020, 45 children with bacterial infection were selected as the bacterial infection group, 45 children with viral infection were selected as the viral infection group, and 50 children who came to our hospital for physical examination at the same time were selected as the control group. All of them received blood routine examination. The WBC count and WBC positive rate of the three groups were compared. Results: WBC of bacterial infection group was (17.97 ± 3.54), significantly higher than that of virus infection group (10.33 ± 3.60) and control group (8.02 ± 2.54) (P < 0.05). There was no significant difference between the virus infection group and the control group (P > 0.05). The positive rate of WBC in bacterial infection group was 80.00%, which was significantly higher than that in virus infection group (42.22%) (P < 0.05). Conclusion: blood routine examination can provide reliable data for clinical diagnosis of bacterial infection in children.展开更多
Objective: to explore the application value of routine urine test in the diagnosis of urinary tract infection for patients with urinary tract infection. Methods: 70 patients with suspected urinary tract infection in o...Objective: to explore the application value of routine urine test in the diagnosis of urinary tract infection for patients with urinary tract infection. Methods: 70 patients with suspected urinary tract infection in our hospital from March 2019 to April 2020 were selected as the research objects. The urine sediment method and urine dry chemistry method were used for examination. The pathological diagnosis results were taken as the gold standard to compare the accuracy of the two methods. Results: with the pathological diagnosis results as gold, whether using urine sediment method or urine dry chemistry method to compare the white blood cell count and red blood cell in urine, it was found that when P < 0.05, there was no statistical significance. The urine sediment method and the urine dry chemical method used in the examination of the patient were tested, and the indicators in the diagnosis of urinary tract infection, such as sensitivity, specificity and accuracy were compared and analyzed, P<0.05, there was no statistical significance. Comparing the two methods, both of them can effectively detect urinary tract infection and provide reference data for subsequent treatment. Conclusion: the urine routine test of patients with urinary tract infection can significantly improve the accuracy rate of patients. The urine dry chemistry method combined with urine sediment method is less different from the gold standard results, which can significantly improve the diagnostic accuracy of patients, which is worthy of extensive promotion.展开更多
Objective: to explore the application of dry chemistry method and urine sediment method in routine urine test. Methods: from May 2020 to October 2021, a total of 150 patients underwent routine urine test in our hospit...Objective: to explore the application of dry chemistry method and urine sediment method in routine urine test. Methods: from May 2020 to October 2021, a total of 150 patients underwent routine urine test in our hospital. All patients underwent routine urine test by dry chemical method, urinary sediment method and combined method, and the test results were analyzed and compared. Results: there was no difference between dry chemical method and urine sediment method in the indexes of red blood cell, white blood cell and transparent cast. In terms of the positive detection rate of quantitative red blood cells, there was no difference between dry chemical method and urine sediment method, 31.33% for the combined test method, which was significantly higher than the detection rate of the two methods, and the difference was statistically significant (p < 0.01). In terms of quantitative detection rate of leukocytes, urinary sediment method was higher than dry chemical method [30.67%vs.2.67%], p < 0.05;The combined test method was 42.67%, which was higher than the previous two methods and the difference was statistically significant (P < 0.01). As for the accuracy rate of urinary tract infection, the combined test method is 96.67%, the chemical method is 78%, and the urinary sediment method is 83.33%, the former is higher than the latter, the difference is statistically significant (p < 0.01);In terms of sensitivity, the combined test method was 96.88%, which was higher than that of dry chemical method (82.03%) and urinary sediment method (87.5%) (P < 0.01). In terms of specificity, the combined test method was 95.45%, the dry chemical method was 54.55%, and the urine sediment method was 59.09%, the former was higher than the latter, the difference was statistically significant (p < 0.01);In terms of bacterial culture results, the comparison of bacterial culture results of dry chemical method and urine sediment method shows that there is no difference in positive detection between the two detection methods, P > 0.05. The positive rate of the combined test method was significantly higher than that of the other two methods (p < 0.05). Conclusion: in routine urine test, dry chemistry method or urine sediment method alone cannot meet the test requirements, and misdiagnosis is easy to occur in the test results. Therefore, two methods of combined test are needed, which can further improve the accuracy and sensitivity of the test results. Therefore, the dry chemical method combined with urinary sediment method is effective and worthy of wide application in clinical testing.展开更多
Hypercoagulation is not detected in clinical practice with routinely performed blood coagulation tests. More advanced laboratory analyses to detect or monitor hypercoagulation have not yet been introduced into routine...Hypercoagulation is not detected in clinical practice with routinely performed blood coagulation tests. More advanced laboratory analyses to detect or monitor hypercoagulation have not yet been introduced into routine clinical management. Thromboelastography assesses the influence of plasma factors and platelets during all phases of haemostasis, thus permits evaluation of hypo- and hyper- coagulation status. This prospective study included assessment of 35 patients with thrombotic complications （II-nd group）, compared with 34 healthy controls （I-st group）. Haemostasis was analyzed with routine clotting tests： protrombin time （PT）, activated partial thromboplastin time （APTT）, fibrinogen, platelets and rotation thromboelastography （ROTEM~） with measuring time to 20 min. All data are presented as mean and standard deviation （SD）. Statistical comparisons of samples were performed by student＇s t-test. The sensitivity, specificity, positive and negative predictive value of the parameters was calculated by using the receiver operator characteristic （ROC） curves for two groups. There was significant difference （P 〈 0.05） observed in the parameters of ROTEM： clot formation time （CFT）, a-angle, maximum clot firmness （MCF） and thrombodynamic potential index （TPI） in the patient＇s population compared to the healthy controls. No significant difference was observed in CT （ROTEM） and routine coagulation tests when the two groups were compared. Rotation thromboelastography analysis demonstrated to be a reliable method for diagnosis of hypercoagulable state.展开更多
We present in this work how to use the dura palm kernel shell powders as loads for the elaboration by extrusion of PVC tubes. The transformation of dura palm kernel shells into micronized shell powder as well as its c...We present in this work how to use the dura palm kernel shell powders as loads for the elaboration by extrusion of PVC tubes. The transformation of dura palm kernel shells into micronized shell powder as well as its characterization was the subject of recent work. We carried out, the formulations by using the industrial scales of precision, the mixtures of the instrants with an industrial machine of mark HENSHLLE N˚2 MAIN 570762, the routine tests by ATG/DSC then by IRTF, the extrusion of the tubes with an extruder twin-screw with 9 rooms of transformation finally the tests of conformity by the observations and the analyses. We obtained for the formulations the dosage of 0%, 4.01%, 12.54%, 23.03%, 32.01%, 38.01%, 51.02% representing the percentage of hull powder in the mixture, the machine mixing gave perfectly homogeneous powders, the routine tests showed that the hull powder keeps all its properties until the end of the shaping whatever the percentage of hulls contained in the PVC, the extrusion gave tubes for each formulation and the conformity tests gave perfectly round tubes of diameter 90 × 82 mm, a coloration going from white grey at 0% to dark black at 51.02% showing the influence of the carbon black and the color of the shell powder according to its proportion. The infrared of the tubes obtained shows for each formulation a variation of the CH<sub>2</sub> and CH bonds and a decrease of the −OH bonds. The thermogravimetric analyses and the differential calorimetric analyses of the tubes of each formulation, show each time that the quantity of shell powders in the mixture influences the PVC tubes obtained. Thus, we obtain a variation of the phase temperatures according to the dosage, giving from 108.72<span style="white-space:normal;">˚</span>C to 76.56<span style="white-space:normal;">˚</span>C for the glass transition temperature and from 494.71<span style="white-space:normal;">˚</span>C to 414.56<span style="white-space:normal;">˚</span>C for the melting temperature, at the DSC and a progressive decrease of the mass following the heat absorption with each time 4 phases instead of 5 for the unloaded PVC tubes at the ATG according to the dosage.展开更多
基金Project of Guangdong Provincial Medical Science and Technology Research Fund(A2022011)Major Science and Technology Project of Shenzhen Nanshan District Health System(NSZD2023067)Sub-project of Education(Health)Science and Technology Project of Nanshan District Technology Research and Development and Creative Design Project in Shenzhen(NS2022002)。
文摘Objective:To explore the accuracy and stability of the results of peripheral blood routine tests at different time points after anticoagulation and standing,providing a scientific basis for actual clinical work.Methods:In this study,30 patients who visited the hospital in October 2023 were randomly selected and divided into two groups(15 cases in each group).The same collection method was used for routine blood tests.The tests were performed after anticoagulation and standing for 5 minutes,1 hour,and 5 minutes,2 hours respectively,and the routine blood test indicators at different time points were compared.Results:After comparison,there were no significant differences in the results of routine blood tests at 5 minutes after mixing,anticoagulating,and standing peripheral blood and those at 1 hour and 2 hours(P>0.05).Conclusion:The results of peripheral blood after mixing,anticoagulating,and standing for 5 minutes are stable compared with those after standing for 1 hour and 2 hours.In actual work,the pre-test turnaround time can be appropriately extended.
基金the Special Project of State Key Laboratory of Dampness Syndrome of Chinese Medicine:Study on Criteria for Diagnosis of Dampness Syndrome of Idiopathic Membranous Nephropathy,Cohort Study on Pathogenesis and Material Basis of Dampness Syndrome of Idiopathic Membranous Nephropathy,Randomized Controlled Clinical Study of Sanqi Qushi Granule in Treatment of Membranous Nephropathy(No.SZ2021ZZ02,SZ2021ZZ09 and SZ2021ZZ36)the 2020 Guangdong Provincial Science and Technology Innovation Strategy Special Fund:Guangdong-Hong Kong-Macao Joint Lab(No.2020B1212030006)+2 种基金the Natural Science Foundation of Guangdong Province:Study on the Mechanism of Sanqi Qushi Prescription Delaying Podocellular Senescence in Membranous Nephropathy based on Cyclic Guanosine Monophosphate-Adenosine Monophosphate Synthase-Stimulator of Interferon Genes-Nuclear Factor Kappa-B Signaling Pathway(No.2022A1515011628)the Guangzhou Science and Technology Plan Project:to Explore the Mechanism of Treating Membranous Nephropathy from the Perspective of Regulating Amino Acid Metabolism Disorder(No.2023A03J0746)Special Funding for Scientific and Technological Research on Traditional Chinese Medicine,Guangdong Provincial Hospital of Chinese Medicine:a Multimodular Machine Learning Prediction Model based on Pathological Image-transcriptomics and Traditional Chinese Medicine Syndromes was Used to Investigate the Prognostic Correlation of Long non-coding RNA Molecules in Nephropathy and the Intervention Mechanism of Sanqi Qushi Formula,to Investigate the Pathogenesis and Microbiological Mechanism of Dampness Syndrome of Membranous Nephropathy based on the Microecological Changes of Tongue Coating(No.YN2023MB02,YN2023MB10)。
文摘OBJECTIVE:To reach consensus on the diagnostic criteria of syndrome of dampness obstruction in idiopathic membranous nephropathy(IMN)patients by literature research and expert investigation(interviews and a Delphi method).METHODS:Our study was consistent with T/CACM 1336-2020.We searched the monographs and references published in the past 40 years(1983-2022),and established the diagnostic criteria pool of waterdampness syndrome and dampness-turbidity syndrome in Traditional Chinese Medicine(TCM)based on literature by using frequency statistics and correlation analysis.Expert investigation(interview method and two rounds of Delphi method)was used to form the diagnostic criteria of water-dampness syndrome and dampnessturbidity syndrome of idiopathic membranous nephropathy.Clinical diagnostic test research was carried out,and compared with“Diagnostic Criteria for dampness syndrome”(T/CACM 1454-2023)to evaluate the authenticity,reliability and clinical application value of the standard.RESULTS:A total of 122 relevant guides,standards,monographs and documents were included through searching books and Chinese databases.Four experts were interviewed and two rounds of delphi method(75 experts nationwide)were carried out.The experts'opinions are relatively concentrated and the differences are small.Based on the weight of each index,the diagnostic criteria indexes of water-dampness syndrome and dampness-turbidity syndrome were selected.After discussion by the core group members,the diagnostic model of"necessary symptoms and optional symptoms"was established,and the final diagnostic criteria of waterdampness syndrome and dampness-turbidity syndrome were established.One hundred and ninety-one inpatients and outpatients of Guangdong Provincial Hospital of Chinese Medicine from January 2021 to February 2023 were included in Diagnostic test study.There was no statistical difference in gender,age and course of disease(P>0.05).The sensitivity and specificity of the trial standard were 90.34%and 73.33%respectively,while the sensitivity and specificity of T/CACM 1454-2023 were 99.43%and 6.67%,respectively.CONCLUSIONS:The consensus-based diagnostic criteria for IMN can be widely incorporated in TCM.A further clinical study will be conducted to analyze the diagnosis value and cut-off score of our IMN criteria.
文摘Urinalysis is one of the most widely developed clinical tests, which plays a key role in the treatment of various diseases of the urinary system, kidney system and system. Total urine test has the advantages of being easy to use and quick to detect. However, in actual clinical trials, a number of factors, including the timing and quality of urine sample collection, the performance of testing equipment and operational standards, can affect the accuracy of the final test results. Therefore, understanding and mastering the influencing factors of clinical routine urine testing, adopting targeted response strategies in a timely manner, and reducing the influence of urine routine testing by external factors are the key to ensuring the accuracy of urine routine test results, and can also provide scientific and reliable basis for the diagnosis of clinical diseases. This paper further analyzes what are the common influencing factors in clinical urine routine testing, discusses related coping strategies, and how to better grasp and improve the quality of its examination in future work to minimize the impact.
基金supported by the National Natural Science Foundation of China (Nos. 21572091 and 21472075)the Fundamental Research Funds for the Central Universities (No. lzujbky-2016-51) by Ministry of Education of China
文摘By modifying the salicylic-acid moiety with electron-withdrawing or –donating groups, three new terbium complexes（LⅠ·Tb, LⅡ·Tb, L·（Ⅲ）Tb） based on tripodal carboxylate ligands were synthesized. Due to different pull-push electronic effects of ligands, the fluorescence intensities of these terbium complexes significantly varied, that is： LⅡ·Tb 〉L（Ⅲ）·Tb〉 LⅠ·Tb. Meanwhile, the characteristic peaks at 492 nm（5D4→7F6） and 547 nm（5 D4→7F5） showed ＂Off–On–Off＂ fluorescence response to various p H conditions,which indicated that all of them can be used as the highly sensitive pH fluorescent probes. Notably, using LⅡ·Tb with the best fluorescence performance as a probe, some patients＇ urine samples can be easily monitored through the response triggered by pH value. Therefore, LⅡ·Tb has the potential to auxiliarily diagnose some diseases in clinical practice through p H detection of routine urine test.
基金Supported by Ningxia Agricultural Reclamation Group Science and Technology Innovation ProjectNew Feed Technology Promotion Project of Ningxia Agriculture and Rural DepartmentNingxia Feed Industry Expert Technical Service Group Project。
文摘[Objectives]This study was conducted to analyze effects of antimicrobial peptides added to the diet of Tan sheep on their production, slaughter performance and blood composition. [Methods] Ninety two four-month-old Tan sheep were randomly divided into two treatment groups according to their body weight, 46 in each group. The control check group(CK) was feed with conventional diet, and the experimental group was fed with the addition of antimicrobial peptide on the basis of the conventional diet, with the added amount of 2.5 g/sheep per day. The experimental period was 60 d. [Results] The incidence rate was 75.06% lower in the experimental group than in the CK. The average daily weight gain per sheep was 11.27% higher in the experimental group than in the CK(P<0.05). The feed conversion ratio was 8.45% lower in the experimental group than in the CK(P<0.05). The average daily gross profit per sheep was 12.12% higher in the experimental group than in the CK. For slaughter performance, the data difference of each item was not significant. The PH at 45 min and 24 h after slaughter was within the normal range of fresh mutton. The cooked meat percentage and water loss rate showed no significant differences(P>0.05). The marbling ranged from 2.45 to 2.50, indicating that the fat content was moderate, and the difference between groups was not significant(P>0.05). The flesh color ranged from 3.00 to 3.15, between light red and bright red, belonging to the normal color of mutton, and the difference between groups was not significant(P>0.05). The shear force was between 2.50 and 2.65, without a significant difference between groups(P>0.05). The white blood cells, lymphocytes and platelets in the experimental group were lower than those in the CK(P<0.01). The erythrocytes and hemoglobin in the erythrocyte group were higher than those in the CK(P<0.05). The neutrophils and monocytes in the experimental group were lower than those in the CK(P<0.05). [Conclusions] This study provides a technical basis for the rational use of antimicrobial peptides and their application in ruminants.
文摘Objective: to explore the application of life test and routine test in the diagnosis of diabetes. Methods: 100 patients suspected of diabetic diseases were selected for biochemical test and routine test, and the results were compared. Results: compared with routine test, biochemical test has higher sensitivity, specificity and accuracy with significant difference (P<0.05). Conclusion: biochemical test has more clinical value in the diagnosis of diabetes.
文摘To study and analyze the influence of peripheral blood and venous blood on the test results in blood routine test. Methods: from September 2020 to June 2021, 152 patients who underwent blood routine test were included in the laboratory department of our hospital. All patients collected peripheral blood and venous blood for blood routine test respectively. The blood source of peripheral blood for blood routine test was peripheral blood group, and the blood source of elbow vein blood for blood routine test was venous blood group, The differences of blood routine test results between the two groups at 0 min were compared, and the blood sources of the two groups were tested for many times at 0 min, 10 min and 30 min after blood collection. The test results were analyzed and compared, and the satisfaction of the two blood collection methods, time and pain were evaluated. Results: at 0 min of blood collection, the leukocyte count value of venous blood group was lower than that of peripheral blood group, while the erythrocyte count value, hemoglobin concentration value, platelet count value, erythrocyte backlog value and average hemoglobin concentration value of venous blood group were higher than those of peripheral blood group (P < 0.05);However, there was no significant difference between the two groups (P > 0.05);The test results of peripheral blood group and venous blood group were compared for multiple tests at 0 min, 10 min and 30 min after blood collection respectively. There was no significant difference in the test results of erythrocyte count and hemoglobin concentration in peripheral blood group at each time period (P > 0.05), while the test results of leukocyte count and platelet count at 0 min were different from those at 10 min. There was significant difference in the results of 30 min test (P < 0.05), indicating that the index value will change with time;There was no significant difference in the test results of leukocyte count, erythrocyte count, hemoglobin concentration and platelet count in the venous blood group (P > 0.05), indicating that the indexes in the blood were relatively stable;Comparing the satisfaction scores of the two groups, the scores of the two groups were higher, and the satisfaction of peripheral blood was slightly higher, but there was no significant difference between the two groups (P > 0.05).Conclusion: comparing the satisfaction of the two groups of patients, the acceptability of the two blood collection methods is very high. From the analysis of the test results, on the whole, the test results of peripheral blood are slightly lower than those of venous blood. It is analyzed that the reason for this result may be that the peripheral blood causes the damage of blood cells during the extrusion process and reduces the count, and the white blood cell count. The platelet count value will change over time and affect the test results, while the venous blood test results have little difference within 30min, indicating that various indexes in venous blood are relatively stable within a certain period of time, and the accuracy of test results is higher.
文摘Objective: the differences of blood routine test results between venous blood and capillary blood were analyzed. Methods: The subjects of the study were 120 healthy outpatients from August 2019 to August 2021. They were divided into two groups according to the random method (n=60 for each group). The capillary blood was collected in the control group and venous blood was collected in the observation group. The results of WBC, HCT, RBC, Hb, PLT, MCHC, MCV and HCT were statistically compared between the two groups. Results: WBC of the observation group was lower than that of the control group, HCT, RBC, Hb, PLT and MCHC of the observation group were higher than those of the control group, p < 0.05 (the difference was statistically significant). Compared with the control group, the MCV and HCT of the observation group had no significant difference (P>0.05). Conclusion: venous blood collection in blood routine test can improve the accuracy of test results, and can be the preferred collection method in blood collection with high clinical value.
文摘Objective: to discuss the clinical value of new hemolysin and traditional hemolysin in blood routine examination. Methods: a total of 89 blood routine test subjects were selected from our hospital. The average control group (45 cases, using traditional hemolysin) and the study group (44 cases, using new hemolysin) were compared. Results: there were no statistically significant differences in the levels of red blood cells, white blood cells and platelets between the two groups (P > 0.05). The level of hemoglobin and satisfaction score in the study group were significantly higher than those in the control group (P < 0.05). The test time and cost in the study group were significantly lower than those in the control group (P < 0.05). Conclusion: the new hemolysin has more application value in routine blood test.
文摘Objective: this study focuses on the main causes and solutions of the routine blood test. Methods: in this study, 120 patients with the error in the routine blood test results from March 2020 to March 2021 were randomly selected as the study subjects, and the clinical data of all the study subjects were analyzed retrospectively. All patients received 2mL blood collection from peripheral blood and 14ml of venous blood. The venous blood samples were divided into 7 groups, among which group 1 was tested immediately after collection, groups 2 and 3 were stored in refrigerator (4℃) and room temperature, groups 4 and 4 hours respectively, group 6 and 7 were treated with 1.6 mg/ml and 2.8 mg/ml anticoagulant respectively. Peripheral blood needs to be tested immediately. The root causes of common errors are summarized, and the influence of different blood collection location, placement time and anticoagulant concentration on the routine blood test indicators is discussed, and the corresponding solutions are summarized. Results: the results of this study showed that platelet content in peripheral blood samples was significantly lower than venous blood, with significantly different data between groups (P <0.05). Blood samples were retained for 2 hours after collection, the hemoglobin concentration and platelet content were lower than the 4-hour retained samples, and the data differences between groups were significant (P <0.05).Blood samples were stored in the refrigerator, the hemoglobin concentration was lower than those stored at room temperature, and there were significant differences between groups (P <0.05).After the blood samples were treated with 2.8 mg/ml anticoagulant concentration, the content of each index was significantly higher than that treated with 1.6 mg/ml, and the data between the two groups varied significantly (P <0.05).Among the routine blood tests, 120 subjects had the error in the routine blood test indicators, including the unreasonable anticoagulant concentration, long inspection time, unreasonable storage and personal factors of the subjects. Conclusion: in the routine blood test, many factors resulted in the test error. The hospital should clarify the main influencing factors, improve the efficiency and quality of routine blood test by strengthening the training of relevant personnel, actively communicating with the subjects, and making reasonable use of anticoagulant, reducing the probability of error, and provide reliable support for the clinical diagnosis and treatment of doctors.
文摘Objective: to analyze the diagnostic value of blood routine examination in children with bacterial infection. Methods: the clinical data of children with infectious diseases examined in our hospital were retrospectively analyzed. From March 2019 to March 2020, 45 children with bacterial infection were selected as the bacterial infection group, 45 children with viral infection were selected as the viral infection group, and 50 children who came to our hospital for physical examination at the same time were selected as the control group. All of them received blood routine examination. The WBC count and WBC positive rate of the three groups were compared. Results: WBC of bacterial infection group was (17.97 ± 3.54), significantly higher than that of virus infection group (10.33 ± 3.60) and control group (8.02 ± 2.54) (P < 0.05). There was no significant difference between the virus infection group and the control group (P > 0.05). The positive rate of WBC in bacterial infection group was 80.00%, which was significantly higher than that in virus infection group (42.22%) (P < 0.05). Conclusion: blood routine examination can provide reliable data for clinical diagnosis of bacterial infection in children.
文摘Objective: to explore the application value of routine urine test in the diagnosis of urinary tract infection for patients with urinary tract infection. Methods: 70 patients with suspected urinary tract infection in our hospital from March 2019 to April 2020 were selected as the research objects. The urine sediment method and urine dry chemistry method were used for examination. The pathological diagnosis results were taken as the gold standard to compare the accuracy of the two methods. Results: with the pathological diagnosis results as gold, whether using urine sediment method or urine dry chemistry method to compare the white blood cell count and red blood cell in urine, it was found that when P < 0.05, there was no statistical significance. The urine sediment method and the urine dry chemical method used in the examination of the patient were tested, and the indicators in the diagnosis of urinary tract infection, such as sensitivity, specificity and accuracy were compared and analyzed, P<0.05, there was no statistical significance. Comparing the two methods, both of them can effectively detect urinary tract infection and provide reference data for subsequent treatment. Conclusion: the urine routine test of patients with urinary tract infection can significantly improve the accuracy rate of patients. The urine dry chemistry method combined with urine sediment method is less different from the gold standard results, which can significantly improve the diagnostic accuracy of patients, which is worthy of extensive promotion.
文摘Objective: to explore the application of dry chemistry method and urine sediment method in routine urine test. Methods: from May 2020 to October 2021, a total of 150 patients underwent routine urine test in our hospital. All patients underwent routine urine test by dry chemical method, urinary sediment method and combined method, and the test results were analyzed and compared. Results: there was no difference between dry chemical method and urine sediment method in the indexes of red blood cell, white blood cell and transparent cast. In terms of the positive detection rate of quantitative red blood cells, there was no difference between dry chemical method and urine sediment method, 31.33% for the combined test method, which was significantly higher than the detection rate of the two methods, and the difference was statistically significant (p < 0.01). In terms of quantitative detection rate of leukocytes, urinary sediment method was higher than dry chemical method [30.67%vs.2.67%], p < 0.05;The combined test method was 42.67%, which was higher than the previous two methods and the difference was statistically significant (P < 0.01). As for the accuracy rate of urinary tract infection, the combined test method is 96.67%, the chemical method is 78%, and the urinary sediment method is 83.33%, the former is higher than the latter, the difference is statistically significant (p < 0.01);In terms of sensitivity, the combined test method was 96.88%, which was higher than that of dry chemical method (82.03%) and urinary sediment method (87.5%) (P < 0.01). In terms of specificity, the combined test method was 95.45%, the dry chemical method was 54.55%, and the urine sediment method was 59.09%, the former was higher than the latter, the difference was statistically significant (p < 0.01);In terms of bacterial culture results, the comparison of bacterial culture results of dry chemical method and urine sediment method shows that there is no difference in positive detection between the two detection methods, P > 0.05. The positive rate of the combined test method was significantly higher than that of the other two methods (p < 0.05). Conclusion: in routine urine test, dry chemistry method or urine sediment method alone cannot meet the test requirements, and misdiagnosis is easy to occur in the test results. Therefore, two methods of combined test are needed, which can further improve the accuracy and sensitivity of the test results. Therefore, the dry chemical method combined with urinary sediment method is effective and worthy of wide application in clinical testing.
文摘Hypercoagulation is not detected in clinical practice with routinely performed blood coagulation tests. More advanced laboratory analyses to detect or monitor hypercoagulation have not yet been introduced into routine clinical management. Thromboelastography assesses the influence of plasma factors and platelets during all phases of haemostasis, thus permits evaluation of hypo- and hyper- coagulation status. This prospective study included assessment of 35 patients with thrombotic complications （II-nd group）, compared with 34 healthy controls （I-st group）. Haemostasis was analyzed with routine clotting tests： protrombin time （PT）, activated partial thromboplastin time （APTT）, fibrinogen, platelets and rotation thromboelastography （ROTEM~） with measuring time to 20 min. All data are presented as mean and standard deviation （SD）. Statistical comparisons of samples were performed by student＇s t-test. The sensitivity, specificity, positive and negative predictive value of the parameters was calculated by using the receiver operator characteristic （ROC） curves for two groups. There was significant difference （P 〈 0.05） observed in the parameters of ROTEM： clot formation time （CFT）, a-angle, maximum clot firmness （MCF） and thrombodynamic potential index （TPI） in the patient＇s population compared to the healthy controls. No significant difference was observed in CT （ROTEM） and routine coagulation tests when the two groups were compared. Rotation thromboelastography analysis demonstrated to be a reliable method for diagnosis of hypercoagulable state.
文摘We present in this work how to use the dura palm kernel shell powders as loads for the elaboration by extrusion of PVC tubes. The transformation of dura palm kernel shells into micronized shell powder as well as its characterization was the subject of recent work. We carried out, the formulations by using the industrial scales of precision, the mixtures of the instrants with an industrial machine of mark HENSHLLE N˚2 MAIN 570762, the routine tests by ATG/DSC then by IRTF, the extrusion of the tubes with an extruder twin-screw with 9 rooms of transformation finally the tests of conformity by the observations and the analyses. We obtained for the formulations the dosage of 0%, 4.01%, 12.54%, 23.03%, 32.01%, 38.01%, 51.02% representing the percentage of hull powder in the mixture, the machine mixing gave perfectly homogeneous powders, the routine tests showed that the hull powder keeps all its properties until the end of the shaping whatever the percentage of hulls contained in the PVC, the extrusion gave tubes for each formulation and the conformity tests gave perfectly round tubes of diameter 90 × 82 mm, a coloration going from white grey at 0% to dark black at 51.02% showing the influence of the carbon black and the color of the shell powder according to its proportion. The infrared of the tubes obtained shows for each formulation a variation of the CH<sub>2</sub> and CH bonds and a decrease of the −OH bonds. The thermogravimetric analyses and the differential calorimetric analyses of the tubes of each formulation, show each time that the quantity of shell powders in the mixture influences the PVC tubes obtained. Thus, we obtain a variation of the phase temperatures according to the dosage, giving from 108.72<span style="white-space:normal;">˚</span>C to 76.56<span style="white-space:normal;">˚</span>C for the glass transition temperature and from 494.71<span style="white-space:normal;">˚</span>C to 414.56<span style="white-space:normal;">˚</span>C for the melting temperature, at the DSC and a progressive decrease of the mass following the heat absorption with each time 4 phases instead of 5 for the unloaded PVC tubes at the ATG according to the dosage.
