[Objective]To systematically isolate and purify the polysaccharide from the mycelium of Streptomyces rochei D74(SRP),elucidate its fine structure,and evaluate the effect of the purified polysaccharide fraction on the ...[Objective]To systematically isolate and purify the polysaccharide from the mycelium of Streptomyces rochei D74(SRP),elucidate its fine structure,and evaluate the effect of the purified polysaccharide fraction on the growth of Salvia miltiorrhiza hairy roots and the biosynthesis of tanshinones,along with the underlying mechanism.[Methods]The crude polysaccharide was extracted using hot water,which was followed by ethanol precipitation and deproteinization via the Sevag method.Further purification was performed using DEAE-52 anionexchange chromatography and Sephadex G-100 gel filtration chromatography.The physicochemical properties and structural features of the main active fraction,SRP-W-2,were systematically characterized by Fourier transform infrared spectroscopy(FTIR),high performance liquid chromatography-mass spectrometry(HPLC-MS),and nuclear magnetic resonance(NMR).The effects of SRP-W-2 on hairy root growth and the biosynthesis of tanshinones were assessed by measuring biomass,tanshinone content,and the expression levels of key biosynthetic genes.[Results]SRP-W-2 was obtained with a yield of 2.41%.It was primarily composed of glucose and galactose at a molar ratio of 12.53:1.Structural analysis revealed that the backbone of SRP-W-2 consisted of→4)-α-D-Glcp-(1→and→4)-α-D-Galp-(1→residues,with branching points at→4,6)-α-D-Glcp-(1→and→4,6)-α-D-Galp-(1→.The side chain was identified asα-D-Glcp-(1→4)-α-DGlcp-(1→.Bioactivity assays demonstrated that SRP-W-2 significantly enhanced both the biomass of S.miltiorrhiza hairy roots and the accumulation of tanshinones.After 15 d of treatment with 50 mg/L SRP-W-2,the dry weight of the hairy roots increased by 37.52%.Meanwhile,the content of cryptotanshinone(CT),dihydrotanshinone I(DT-I),tanshinone I(T-I),and tanshinone IIA(TIIA)was increased by 19.0-fold,6.4-fold,2.8-fold,and 4.8-fold,respectively.Gene expression analysis further indicated that SRP-W-2 up-regulated key genes involved in the tanshinone biosynthetic pathway,including HMGR,DXS,DXR,and GGPPS.[Conclusion]The polysaccharide fraction SRP-W-2 from S.rochei D74 simultaneously promoted the growth of S.miltiorrhiza hairy roots and the biosynthesis of tanshinones,demonstrating its potential as an effective elicitor.This study provided a new strategy for the utilization and development of S.miltiorrhiza resources.展开更多
Green manuring is essential for improving soil quality and nutrient uptake.With the gradual depletion of phosphorus(P)resources,more attention is being paid to the role of green manures in cultivation systems,such as ...Green manuring is essential for improving soil quality and nutrient uptake.With the gradual depletion of phosphorus(P)resources,more attention is being paid to the role of green manures in cultivation systems,such as maize-green manure intercropping,to find possible pathways for enhancing soil P utilization.A maize-green manure intercropping experiment was started in 2009 to investigate the effects and mechanisms for enhancing P uptake and yield in maize.Three species of green manures(hairy vetch(HV),needle leaf pea(NP),sweet pea(SP))and a sole maize treatment(CK)were used,resulting in four treatments(CK,HVT,NPT,and SPT)in the experiment.During 2020-2023,the intercropping treatments enhanced maize yields in 2020 and 2021,particularly in HVT with increases of 13.7%(1.96 t ha^(-1))and 13.0%(2.13 t ha^(-1))compared with CK,respectively.Grain P accumulation of maize was significantly higher in the intercropping treatments than CK in 2020,2021,and 2023,and with an average increase of 10.6%over the four years(5.2% for NPT,10.8% for SPT and 15.9% for HVT)compared with CK.Intercropping promoted maize growth with a greater root length density and a higher organic acid release rate.HVT changed the soil properties more dramatically than the other treatments,with increases in the acid phosphatase and alkaline phosphatase activities of 29.8 and 38.5%,respectively,in the topsoil(0-15 cm),while the soil p H was reduced by 0.37 units compared to CK(p H=8.44).Intercropping treatments facilitated the conversion of non-labile P to mod-labile P and stimulated the growth of soil bacteria in the topsoil.Compared with CK,the relative abundance of Gemmatimonadota,known for accumulating polyphosphate,and Actinobacteriota,a prominent source of bioactive compounds,increased significantly in the intercropping treatments,especially in HVT and SPT.A PLS-PM analysis showed that intercropping promoted soil P mobilization and the enrichment of beneficial bacteria by regulating maize root morphology and physiology.Our results highlight that maize-green manure intercropping optimizes root traits,soil properties and bacterial composition,which contribute to greater maize P uptake and yield,providing an effective strategy for sustainable crop production.展开更多
Few studies have investigated alterations in the immune cell microenvironment of the dorsal root ganglia following spinal cord injury and whether these modifications facilitate axonal regeneration.In this study,we use...Few studies have investigated alterations in the immune cell microenvironment of the dorsal root ganglia following spinal cord injury and whether these modifications facilitate axonal regeneration.In this study,we used a single-cell RNA sequencing dataset to create a comprehensive profile of the diverse cell types in the dorsal root ganglia and spinal cord of a mid-thoracic contusion injury model in cynomolgus monkeys.Cell communication analysis indicated that specific signaling events among various dorsal root ganglia cell types occur in response to spinal cord injury.Single-cell analysis using dimensionality reduction clustering identified distinct molecular signatures for nine cell types,including macrophage subpopulations,and differential gene expression profiles between dorsal root ganglia cells and spinal cord cells following spinal cord injury.The macrophage subpopulations were categorized into 11 clusters(MC0-MC10)based on differentially expressed genes,with the top 10 genes being ABCA6,RBMS3,EBF1,LAMA4,ANTXR2,LAMA2,SOX5,FOXP2,GHR,and APOD.MC0,MC1,and MC2 constituted the predominant macrophage populations.MC4,MC6,and MC9 were nearly absent in the spinal cord,but exhibited significant increases in the dorsal root ganglia post-spinal cord injury.Notably,these subpopulations possess a strong capacity for regulating axonal regeneration.The developmental progression of dorsal root ganglia macrophages after spinal cord injury was elucidated using cell trajectory and pseudo-time analyses.Genes such as EBF1(MC6 and MC9 marker),RBMS3(MC6 and MC9 marker),and ABCA6(MC6 marker)showed high expression levels in the critical pathways of macrophage function.Through ligand-receptor pair analysis,we determined that the effects of macrophages on microglia are predominantly mediated through interaction pairs(e.g.,SPP1-CD44,LAMC1-CD44,and FN1-CD44),potentially facilitating specific cellular communications within the immune microenvironment.The single-cell RNA sequencing dataset used in this study represents the first comprehensive transcriptional analysis of the dorsal root ganglia after spinal cord injury in cynomolgus monkeys,encompassing nearly all cell types within the dorsal root ganglia region.Using this dataset,we evaluated diverse subtypes of macrophages in the post-spinal cord injury dorsal root ganglia area and examined the signaling pathways that facilitate interactions among immune response-related macrophages in the dorsal root ganglia.Findings from this study provide a theoretical basis for understanding how the immune microenvironment influences the regenerative capacity of dorsal root ganglia neurons after spinal cord injury and offer novel insights into the complex processes underlying the pathobiology of spinal cord injury.展开更多
To characterize effects of plant roots on preferential flow（PF）,we measured root length density（RLD）and root biomass（RB） in Jiufeng National Forest Park,Beijing,China.Comparisons were made for RLD and RB between...To characterize effects of plant roots on preferential flow（PF）,we measured root length density（RLD）and root biomass（RB） in Jiufeng National Forest Park,Beijing,China.Comparisons were made for RLD and RB between soil preferential pathways and soil matrices.RLD and RB declined with the increasing soil depth（0–10,10–20,20–30,30–40,40–50,50–60 cm） in all experimental plots.RLD was greater in soil preferential pathways than in the surrounding soil matrix and was 69.5,75.0 and72.2 % for plant roots of diameter（d） ／1,1 ／ d ／ 3 and3 ／ d ／ 5 mm,respectively.Fine root systems had the most pivotal influence on soil preferential flow in this forest ecosystem.In all experimental plots,RB content was the sum of RB from soil preferential pathways and the soil matrix in each soil depth.With respect to 6 soil depth gradient（0–10,10–20,20–30,30–40,40–50,50–60 cm） in each plot,the number of soil depth gradient that RB content was greater in soil preferential pathways than in the soil matrix was characterized,and the proportion was68.2 % in all plots.展开更多
Soil alkalinity is a major factor that restricts the growth of apple roots.To analyze the response of apple roots to alkali stress, the root structure and endogenous hormones of two apple rootstocks, Malus prunifolia ...Soil alkalinity is a major factor that restricts the growth of apple roots.To analyze the response of apple roots to alkali stress, the root structure and endogenous hormones of two apple rootstocks, Malus prunifolia (alkali-tolerant) and Malus hupehensis (alkali-sensitive), were compared. To understand alkali tolerance of M. prunifolia at the molecular level, transcriptome analysis was performed. When plants were cultured in alkaline conditions for 15 d, the root growth of M. hupehensis with weak alkali tolerance decreased significantly. Analysis of endogenous hormone levels showed that the concentrations of indole-3-acetic acid (IAA) and zeatin riboside (ZR) in M. hupehensis under alkali stress were lower than those in the control. However, the trend for IAA and ZR in M. prunifolia was the opposite. The concentration of abscisic acid (ABA) in the roots of the two apple rootstocks under alkali stress increased, but the concentration of ABA in the roots of M. prunifolia was higher than that in M. hupehensis. The expression of IAA-related genes ARF5, GH3.6, SAUR36, and SAUR32 and the Cytokinin (CTK)-related gene IPT5 in M. prunifolia was higher than those in the control, but the expression of these genes in M. hupehensis was lower than those in the control. The expression of ABA-related genes CIPK1 and AHK1 increased in the two apple rootstocks under alkali stress, but the expression of CIPK1 and AHK1 in M. prunifolia was higher than in M. hupehensis. These results demonstrated that under alkali stress, the increase of IAA, ZR, and ABA in roots and the increase of the expression of related genes promoted the growth of roots and improved the alkali tolerance of apple rootstocks.展开更多
Non-heading Chinese cabbage, a variety of Brassica campestris, is an important vegetable crop in the Yangtze River Basin of China. However,the immaturity of its stable transformation system and its low transformation ...Non-heading Chinese cabbage, a variety of Brassica campestris, is an important vegetable crop in the Yangtze River Basin of China. However,the immaturity of its stable transformation system and its low transformation efficiency limit gene function research on non-heading Chinese cabbage. Agrobacterium rhizogenes-mediated(ARM) transgenic technology is a rapid and effective transformation method that has not yet been established for non-heading Chinese cabbage plants. Here, we optimized conventional ARM approaches(one-step and two-step transformation methods) suitable for living non-heading Chinese cabbage plants in nonsterile environments. Transgenic roots in composite non-heading Chinese cabbage plants were identified using phenotypic detection, fluorescence observation, and PCR analysis. The transformation efficiency of a two-step method on four five-day-old non-heading Chinese cabbage seedlings(Suzhouqing, Huangmeigui, Wuyueman, and Sijiu Caixin) was 43.33%-51.09%, whereas using the stout hypocotyl resulted in a transformation efficiency of 54.88% for the 30-day-old Sijiu Caixin.The one-step method outperformed the two-step method;the transformation efficiency of different varieties was above 60%, and both methods can be used to obtain transgenic roots for functional studies within one month. Finally, optimized ARM transformation methods can easily,quickly, and effectively produce composite non-heading Chinese cabbage plants with transgenic roots, providing a reliable foundation for gene function research and non-heading Chinese cabbage genetic improvement breeding.展开更多
[Objective] Iron deficiency is one of the most important crop element deficiencies in the Loess Plateau of northwestern China. The selection for crop cultivars that are tolerant to low iron levels could be one of the ...[Objective] Iron deficiency is one of the most important crop element deficiencies in the Loess Plateau of northwestern China. The selection for crop cultivars that are tolerant to low iron levels could be one of the approaches to solving the problem and improving crop production. [Method] Three major apple root stock species （Malus prunifolia, Malus sieversii and Malus baccata） were selected to evaluate their tolerance to iron defciency in hydroponic system. A 3×2 factorial pot experiment was conducted with three replicates in a greenhouse at Gansu Agricultural University, Lanzhou, China. [Result] The SOD, POD and CAT activities in roots and stems of the 3 root stock species in Fe-defcient Hoagland solution decreased, however Malus sieversii got the less reduction and had better root architecture and growth than the other species. The aboveground biomass, plant height, chlorophyll content, total root length and lateral root number were correlated positively with iron-defciency stress tolerance. The species’ tolerance to iron-defciency from high to low was M. sieversii’s〉M. baccata’s〉M. prunifolia’s. Moreover, the improvement of some morphological features such as root length, above-ground biomass, plant height and lateral root number in apple could be conducive to breeding cultivars with tolerance to iron-defciency stress. [Conclusion] Malus sieversii had better tolerance to iron-defciency stress than the others in this study.展开更多
Based on the survey of 154 farmers in Guiyang City, we analyze the basic situation of building of grass-roots agricultural technology extension system in Guiyang City. Then we point out some problems in the building o...Based on the survey of 154 farmers in Guiyang City, we analyze the basic situation of building of grass-roots agricultural technology extension system in Guiyang City. Then we point out some problems in the building of grass-roots agricultural technology extension system in Guiyang City: the function of grass-roots agricultural technology extension center weakens; the content of agricultural technology extension is difficult to adapt to farmers' needs for technology; the extension mode of agricultural technology departments does not adapt to the needs of modern agriculture. In order to perfect the building of grass-roots agricultural technology extension system, the countermeasures and recommendations are put forth as follows: strengthening the input of funds, and ensuring that the basic work of public welfare agricultural technology extension is smoothly carried out; innovating upon the system, and improving the function of grass-roots agricultural technology extension center; implementing management on agricultural technology extension personnel's performance, and promoting the extension efficiency; strengthening the building of extension team in rural areas, and cultivating high-quality agricultural technology extension personnel; exploring the advanced service mode to meet farmers' needs.展开更多
Genetic transformation experiments of the different explants from Citrus grandis cv. Changshou Shatian You infected with Agrobacterium rhizogenes were carried out in darkness or in light. The optimizing culture system...Genetic transformation experiments of the different explants from Citrus grandis cv. Changshou Shatian You infected with Agrobacterium rhizogenes were carried out in darkness or in light. The optimizing culture system of Ri T-DNA transformed roots for C. grandis cv. Changshou Shatian You was constructed as follows： After the ventral wounded striations on the single activation cotyledon were inoculated by A. rhizogenes A4 （logarithmic period）, they were cocultured at （25 ±2）℃ in darkness for 25-30 days; some transformed roots were generated from wounded striations of most cotyledons. The genetically transformed ratio is （83 ± 11）%. Axenic Ri T-DNA transformed roots （hairy roots） were harvested after five subcultures. Explants were activated on MT medium. The MS medium was used for subculture of transformed roots. Mass Ri T-DNA transformed roots in which the hormone was produced independently were harvested from this optimizing culture system. White, fresh Ri T-DNA transformed roots were （1.14 ±0.07） cm long, （0.73 ±0.04） mm wide, and the growth direction of transformed roots was negative geotropism.展开更多
Climate change has significantly increased the frequency and severity of droughts and risk of tree death worldwide.Differences in leaf habit,plant size,and species diversity are associated with differences in the risk...Climate change has significantly increased the frequency and severity of droughts and risk of tree death worldwide.Differences in leaf habit,plant size,and species diversity are associated with differences in the risk of drought-induced mortality,but the relative contributions of these factors to the risk of mortality are unclear.In a study of the mortality of tree and shrub species during the extreme drought of 2019 in a savanna ecosystem in Southwest China,we assessed the relative contributions of evergreen and deciduous leaf habit,plant size,and species richness and diversity to the mortality of shrubs and trees after the 2019 extreme drought.The deciduous species had significantly lower hydraulic safety margins than the coexisting evergreen species,resulting in a higher mortality risk.Additionally,species and individuals with taller canopies tended to have deeper root systems,an advantage during extreme drought that reduced mortality risk.Notably,mortality risk was largely independent of stand species richness and diversity.Overall,leaf habit and plant height were better predictors of mortality risk than species richness and diversity.These novel insights provide a better understanding of the mechanisms driving drought-induced mortality in the ecosystems with a low canopy and weak interspecific and intraspecific competition for shared resources.Leaf habit and tree size should be incorporated into hypotheses on the mechanisms underlying drought-induced tree mortality.展开更多
The Qinghai-Xizang Plateau of China faces challenges like thaw slumping,threatening slope stability and infrastructure.Understanding the mechanical properties of the roots of the dominant herbaceous plant species in t...The Qinghai-Xizang Plateau of China faces challenges like thaw slumping,threatening slope stability and infrastructure.Understanding the mechanical properties of the roots of the dominant herbaceous plant species in the alpine meadow layer of the permafrost regions on the Qinghai-Xizang Plateau is essential for evaluating their role in enhancing soil shear strength and mitigating slope deformation in these fragile environments.In this study,the roots of four dominant herbaceous plant species—Kobresia pygmaea,Kobresia humilis,Carex moorcroftii,and Leontopodium pusillum—that are widely distributed in the permafrost regions of the Qinghai-Xizang Plateau were explored to determine their mechanical properties and effects in enhancing soil shear strength.Through indoor single root tensile and root group tensile tests,we determined the root diameter,tensile force,tensile strength,tensile ratio,and strength frequency distributions.We also evaluated their contributions to inhibiting slope deformation and failure during the formation and development of thermal thaw slumps in the alpine meadow.The results showed that the distribution of the root diameter of the dominant plant species is mostly normal,while the tensile strength tends to be logarithmically normally distributed.The relationship between the root diameter and root tensile strength conforms to a power function.The theoretical tensile strength of the root group was calculated using the Wu-Waldron Model(WWM)and the Fiber Bundle Model(FBM)under the assumption that the cumulative single tensile strength of the root bundle is identical to the tensile strength of the root group in the WWM.The FBM considers three fracture modes:FBM-D(the tensile force on each single root is proportional to its diameter relative to the total sum of all the root diameters),FBM-S(the cross-sectional stress in the root bundle is uniform),and FBM-N(each tensile strength test of individual roots experiences an equal load).It was found that the model-calculated tensile strength of the root group was 162.60%higher than the test value.The model-derived tensile force of the root group from the FBM-D,FBM-S,and FBM-N was 73.10%,28.91%,and 13.47%higher than the test values,respectively.The additional cohesion of the soil provided by the roots was calculated to be 25.90-45.06 kPa using the modified WWM,67.05-38.15 kPa using the FBM-S,and 57.24-32.74 kPa using the FBM-N.These results not only provide a theoretical basis for further quantitative evaluation of the mechanical effects of the root systems of herbaceous plant species in reinforcing the surface soil but also have practical significance for the effective prevention and control of thermal thaw slumping disasters in the permafrost regions containing native alpine meadows on the Qinghai-Xizang Plateau using flexible plant protection measures.展开更多
Plant root systems,a crucial component of biogeotechnics,have been recognized as a promising and sustainable strategy to address novel challenges in geotechnical engineering,i.e.,climate change(Ng et al.,2022).Root-so...Plant root systems,a crucial component of biogeotechnics,have been recognized as a promising and sustainable strategy to address novel challenges in geotechnical engineering,i.e.,climate change(Ng et al.,2022).Root-soil composite and root-reinforced slopes have re-ceived widespread attention in recent decades,due to the ability of root to regulate soil properties through mechanical reinforcement and hy-draulic transpiration(Li&Duan,2023;Ni et al.,2024).Fig.1 provides a co-occurrence network plot of plant root-based soil reinforcement strategies published over the last decade,where three clusters are identified with different colors.On the left of the network map,clusters in red and blue are primarily driven by geotechnical investigations of vegetated slopes(i.e.,plant root reinforced slopes)and root-soil com-posite/root-permeated soils,as denoted by the terms like"model","test","slope","strength"and"vegetation",while the green cluster on the right side demonstrates botany-related domains,for instance,"plant growth",Indeed,the reinforcement of vegetated soil strength is com-plex and varies significantly with an abundance of factors,both me-chanically and hydraulically.Particularly,the impact of root mor-phology and architecture cannot be negligible,including keywords"root area ratio"root distribution""root morphology"root diame-ter"root density"in Fig.1 with the root size and root depth ranking foremost.展开更多
The article contains a list of 14 structured technical sheets on roots, rhizomes or tubers that will serve as guidance in improving the quality of health of people. The used method was through an active search for lit...The article contains a list of 14 structured technical sheets on roots, rhizomes or tubers that will serve as guidance in improving the quality of health of people. The used method was through an active search for literature that brought the observed aspects to the structuring of the technical sheets, i.e., literature that contains data related to composition, active ingredients, interaction (when described in the consulted literature), health condition in which they may be useful in helath care by various health professionals and anyone who may be of interest. The data obtained shows that there is a need for a careful evaluation of the foods (that contain roots, rhizomes, or tubers) used by polymedicated people due to possible interactions and incompatibilities that may exist.展开更多
Root resorption is a significant complication in orthodontic treatment,with thin roots and reciprocal movement being recognized as high-risk factors.This paper reports a case of a 19-year-old female patient who underw...Root resorption is a significant complication in orthodontic treatment,with thin roots and reciprocal movement being recognized as high-risk factors.This paper reports a case of a 19-year-old female patient who underwent orthodontic treatment for dental irregularity.The patient had thin roots in the maxillary lateral incisors 12 and 22.During treatment,tooth 22 experienced reciprocal movement of labial expansion followed by retraction,while tooth 12 adopted passive ligation to reduce reciprocal movement.After 23 months of straight-wire extraction treatment,good occlusal relationships were achieved,but significant root resorption occurred in teeth 12 and 22,with tooth 22 showing more severe resorption.This case confirms the synergistic effect between thin roots and reciprocal movement,demonstrating that thin roots are more sensitive to reciprocal movement stimulation,producing a synergistic amplification effect.Additionally,standardized nursing guidance and patient compliance management play important roles in reducing resorption risk.This case emphasizes the importance of pretreatment risk assessment,individualized treatment strategy formulation,and comprehensive nursing intervention throughout treatment,providing reference for clinical prevention of root resorption.展开更多
Root system architecture has often been overlooked in plant research despite its critical role in plant adaptation to environmental conditions.This study focused on the root system architecture of the desert shrub Rea...Root system architecture has often been overlooked in plant research despite its critical role in plant adaptation to environmental conditions.This study focused on the root system architecture of the desert shrub Reaumuria soongorica in the Alxa steppe desert,Northwest China.Plant samples were collected during May-September 2019.Using excavation methods,in situ measurements,and root scanning techniques,we analyzed the root distribution,topology,and branching patterns of R.soongorica across an age sequence of 7-51 a.Additionally,we investigated the allometric relationships of root collar diameter with total coarse root length,biomass,and topological parameters.The results showed that the roots of R.soongorica were predominantly concentrated in shallow soil layers(10-50 cm),with lateral root branching and biomass allocation increasing with shrub age.The root topology exhibited a herringbone-like structure,with average topological and modified topological indices of 0.89 and 0.96,respectively,both of which adjusted with shrub age.The root system displayed a self-similar branching pattern,maintaining a constant cross-sectional area ratio of 1.13 before and after branching,deviating from the area-preserving rule.These adaptive traits allow R.soongorica to efficiently expand its nutrient acquisition zone,minimize internal competition,and optimize resource uptake from the upper soil layers.Furthermore,significant linear relationships were observed between log10-transformed root collar diameter and log10-transformed total coarse root length,biomass,and topological parameters.These findings advance non-destructive approaches for studying root characteristics and contribute to the development of root-related models.Besides,this study provides new insights into the adaptive strategies of R.soongorica under extreme drought conditions,offering valuable guidance for species selection and cultivation in desert restoration efforts.展开更多
Two decades ago,an important development philosophy emerged that would redefine prosperity:“Lucid waters and lush mountains are invaluable assets.”Known as the“Two Mountains”theory,it challenged the conventional t...Two decades ago,an important development philosophy emerged that would redefine prosperity:“Lucid waters and lush mountains are invaluable assets.”Known as the“Two Mountains”theory,it challenged the conventional trade-off between development and the environment by arguing that they are inseparable.展开更多
BACKGROUND The numbers of mandibular first premolar roots and root canals vary,and the incidence of three roots and three canals is 0.09%.CASE SUMMARY In this article,we review the root and root canal conditions for t...BACKGROUND The numbers of mandibular first premolar roots and root canals vary,and the incidence of three roots and three canals is 0.09%.CASE SUMMARY In this article,we review the root and root canal conditions for the mandibular first premolar and report the case of a mandibular left first premolar with three roots and three canals in a male patient,with suggestions for clinical diagnosis and treatment.The patient was referred by an orthodontist for the extraction of the tooth.Preoperative cone-beam computed tomography examination revealed that it had three roots.Under local anesthesia,the extraction socket was carefully expanded,and the tooth was successfully removed intact using forceps.The procedure was uneventful,with no root fractures,postoperative bleeding,or sensory abnormality observed.CONCLUSION The mandibular first premolar is characterized by multiple roots and canal variations that can increase the difficulty of treatment.展开更多
As French Tibetologist Alexandra David-Neel noted,"For many centuries the history of Xizang has been inseparable from that of China."①In the long course of China's five-thousand-year history,Xizang and ...As French Tibetologist Alexandra David-Neel noted,"For many centuries the history of Xizang has been inseparable from that of China."①In the long course of China's five-thousand-year history,Xizang and the Central Plains have,through economic exchanges,political interactions and cultural contacts,developed a strong bond.These historically rooted close links are a reflection of the intermingled distribution,cultural inclusiveness.展开更多
Optimizing root system architecture(RSA)is essential for plants because of its critical role in acquiring water and nutrients from the soil.However,the subterranean nature of roots complicates the measurement of RSA t...Optimizing root system architecture(RSA)is essential for plants because of its critical role in acquiring water and nutrients from the soil.However,the subterranean nature of roots complicates the measurement of RSA traits.Recently developed rhizobox methods allow for the rapid acquisition of root images.Nevertheless,effective and precise approaches for extracting RSA features from these images remain underdeveloped.Deep learning(DL)technology can enhance image segmentation and facilitate RSA trait extraction.However,comprehensive pipelines that integrate DL technologies into image-based root phenotyping techniques are still scarce,hampering their implementation.To address this challenge,we present a reproducible pipeline(faCRSA)for automated RSA traits analysis,consisting of three modules:(1)the RSA traits extraction module functions to segment soil-root images and calculate RSA traits.A lightweight convolutional neural network(CNN)named RootSeg was proposed for efficient and accurate segmentation;(2)the data storage module,which stores image and text data from other modules;and(3)the web application module,which allows researchers to analyze data online in a user-friendly manner.The correlation coefficients(R^(2))of total root length,root surface area,and root volume calculated from faCRSA and manually measured results were 0.96**,0.97**,and 0.93**,respectively,with root mean square errors(RMSE)of 8.13 cm,1.68 cm^(2),and 0.05 cm^(3),processed at a rate of 9.74 s per image,indicating satisfying accuracy.faCRSA has also demonstrated satisfactory performance in dynamically monitoring root system changes under various stress conditions,such as drought or waterlogging.The detailed code and deployable package of faCRSA are provided for researchers with the potential to replace manual and semi-automated methods.展开更多
INTRODUCTION Cardiovascular system involvement is an important determinant of long-term prognosis in patients with systemic lupus erythematosus(SLE).Aneurysmal dilatation of the aortic root combined with Stanford type...INTRODUCTION Cardiovascular system involvement is an important determinant of long-term prognosis in patients with systemic lupus erythematosus(SLE).Aneurysmal dilatation of the aortic root combined with Stanford type A aortic dissection(TAAD)is a highly catastrophic complication in these patients.展开更多
文摘[Objective]To systematically isolate and purify the polysaccharide from the mycelium of Streptomyces rochei D74(SRP),elucidate its fine structure,and evaluate the effect of the purified polysaccharide fraction on the growth of Salvia miltiorrhiza hairy roots and the biosynthesis of tanshinones,along with the underlying mechanism.[Methods]The crude polysaccharide was extracted using hot water,which was followed by ethanol precipitation and deproteinization via the Sevag method.Further purification was performed using DEAE-52 anionexchange chromatography and Sephadex G-100 gel filtration chromatography.The physicochemical properties and structural features of the main active fraction,SRP-W-2,were systematically characterized by Fourier transform infrared spectroscopy(FTIR),high performance liquid chromatography-mass spectrometry(HPLC-MS),and nuclear magnetic resonance(NMR).The effects of SRP-W-2 on hairy root growth and the biosynthesis of tanshinones were assessed by measuring biomass,tanshinone content,and the expression levels of key biosynthetic genes.[Results]SRP-W-2 was obtained with a yield of 2.41%.It was primarily composed of glucose and galactose at a molar ratio of 12.53:1.Structural analysis revealed that the backbone of SRP-W-2 consisted of→4)-α-D-Glcp-(1→and→4)-α-D-Galp-(1→residues,with branching points at→4,6)-α-D-Glcp-(1→and→4,6)-α-D-Galp-(1→.The side chain was identified asα-D-Glcp-(1→4)-α-DGlcp-(1→.Bioactivity assays demonstrated that SRP-W-2 significantly enhanced both the biomass of S.miltiorrhiza hairy roots and the accumulation of tanshinones.After 15 d of treatment with 50 mg/L SRP-W-2,the dry weight of the hairy roots increased by 37.52%.Meanwhile,the content of cryptotanshinone(CT),dihydrotanshinone I(DT-I),tanshinone I(T-I),and tanshinone IIA(TIIA)was increased by 19.0-fold,6.4-fold,2.8-fold,and 4.8-fold,respectively.Gene expression analysis further indicated that SRP-W-2 up-regulated key genes involved in the tanshinone biosynthetic pathway,including HMGR,DXS,DXR,and GGPPS.[Conclusion]The polysaccharide fraction SRP-W-2 from S.rochei D74 simultaneously promoted the growth of S.miltiorrhiza hairy roots and the biosynthesis of tanshinones,demonstrating its potential as an effective elicitor.This study provided a new strategy for the utilization and development of S.miltiorrhiza resources.
基金supported financially by the National Key Research&Development Program of China(2021YFD1700200)the National Natural Science Foundation of China(32402686)+3 种基金the Earmarked Fund for China Agriculture Research System(CARS-22)the Fundamental Research Funds for Central Non-profit Scientific Institution,China(1610132022013)the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciencesthe China National Crop Germplasm Resources Platform for Green Manure(NICGR-2024-19)。
文摘Green manuring is essential for improving soil quality and nutrient uptake.With the gradual depletion of phosphorus(P)resources,more attention is being paid to the role of green manures in cultivation systems,such as maize-green manure intercropping,to find possible pathways for enhancing soil P utilization.A maize-green manure intercropping experiment was started in 2009 to investigate the effects and mechanisms for enhancing P uptake and yield in maize.Three species of green manures(hairy vetch(HV),needle leaf pea(NP),sweet pea(SP))and a sole maize treatment(CK)were used,resulting in four treatments(CK,HVT,NPT,and SPT)in the experiment.During 2020-2023,the intercropping treatments enhanced maize yields in 2020 and 2021,particularly in HVT with increases of 13.7%(1.96 t ha^(-1))and 13.0%(2.13 t ha^(-1))compared with CK,respectively.Grain P accumulation of maize was significantly higher in the intercropping treatments than CK in 2020,2021,and 2023,and with an average increase of 10.6%over the four years(5.2% for NPT,10.8% for SPT and 15.9% for HVT)compared with CK.Intercropping promoted maize growth with a greater root length density and a higher organic acid release rate.HVT changed the soil properties more dramatically than the other treatments,with increases in the acid phosphatase and alkaline phosphatase activities of 29.8 and 38.5%,respectively,in the topsoil(0-15 cm),while the soil p H was reduced by 0.37 units compared to CK(p H=8.44).Intercropping treatments facilitated the conversion of non-labile P to mod-labile P and stimulated the growth of soil bacteria in the topsoil.Compared with CK,the relative abundance of Gemmatimonadota,known for accumulating polyphosphate,and Actinobacteriota,a prominent source of bioactive compounds,increased significantly in the intercropping treatments,especially in HVT and SPT.A PLS-PM analysis showed that intercropping promoted soil P mobilization and the enrichment of beneficial bacteria by regulating maize root morphology and physiology.Our results highlight that maize-green manure intercropping optimizes root traits,soil properties and bacterial composition,which contribute to greater maize P uptake and yield,providing an effective strategy for sustainable crop production.
基金supported by the Tianjin Key Medical Discipline(Specialty)Construct Project,No.TJYXZDXK-027A(to SF)the National Key Research andDevelopment Project of Stem Cell and Transformation Research,No.2019YFA0112100(to SF)+2 种基金Tianjin Natural Science Foundation’s Youth Project for DiverseInvestments,No.21JCQNJC01300(to BF)the National Natural Science Foundation of China(Youth Program),No.82102563(to BF)Tianjin Major Science andTechnology Special Projects and Engineering Projects,No.21ZXJBSY00080(to YR).
文摘Few studies have investigated alterations in the immune cell microenvironment of the dorsal root ganglia following spinal cord injury and whether these modifications facilitate axonal regeneration.In this study,we used a single-cell RNA sequencing dataset to create a comprehensive profile of the diverse cell types in the dorsal root ganglia and spinal cord of a mid-thoracic contusion injury model in cynomolgus monkeys.Cell communication analysis indicated that specific signaling events among various dorsal root ganglia cell types occur in response to spinal cord injury.Single-cell analysis using dimensionality reduction clustering identified distinct molecular signatures for nine cell types,including macrophage subpopulations,and differential gene expression profiles between dorsal root ganglia cells and spinal cord cells following spinal cord injury.The macrophage subpopulations were categorized into 11 clusters(MC0-MC10)based on differentially expressed genes,with the top 10 genes being ABCA6,RBMS3,EBF1,LAMA4,ANTXR2,LAMA2,SOX5,FOXP2,GHR,and APOD.MC0,MC1,and MC2 constituted the predominant macrophage populations.MC4,MC6,and MC9 were nearly absent in the spinal cord,but exhibited significant increases in the dorsal root ganglia post-spinal cord injury.Notably,these subpopulations possess a strong capacity for regulating axonal regeneration.The developmental progression of dorsal root ganglia macrophages after spinal cord injury was elucidated using cell trajectory and pseudo-time analyses.Genes such as EBF1(MC6 and MC9 marker),RBMS3(MC6 and MC9 marker),and ABCA6(MC6 marker)showed high expression levels in the critical pathways of macrophage function.Through ligand-receptor pair analysis,we determined that the effects of macrophages on microglia are predominantly mediated through interaction pairs(e.g.,SPP1-CD44,LAMC1-CD44,and FN1-CD44),potentially facilitating specific cellular communications within the immune microenvironment.The single-cell RNA sequencing dataset used in this study represents the first comprehensive transcriptional analysis of the dorsal root ganglia after spinal cord injury in cynomolgus monkeys,encompassing nearly all cell types within the dorsal root ganglia region.Using this dataset,we evaluated diverse subtypes of macrophages in the post-spinal cord injury dorsal root ganglia area and examined the signaling pathways that facilitate interactions among immune response-related macrophages in the dorsal root ganglia.Findings from this study provide a theoretical basis for understanding how the immune microenvironment influences the regenerative capacity of dorsal root ganglia neurons after spinal cord injury and offer novel insights into the complex processes underlying the pathobiology of spinal cord injury.
基金supported by a grant from the Natural Science Foundation of China(41271044)
文摘To characterize effects of plant roots on preferential flow（PF）,we measured root length density（RLD）and root biomass（RB） in Jiufeng National Forest Park,Beijing,China.Comparisons were made for RLD and RB between soil preferential pathways and soil matrices.RLD and RB declined with the increasing soil depth（0–10,10–20,20–30,30–40,40–50,50–60 cm） in all experimental plots.RLD was greater in soil preferential pathways than in the surrounding soil matrix and was 69.5,75.0 and72.2 % for plant roots of diameter（d） ／1,1 ／ d ／ 3 and3 ／ d ／ 5 mm,respectively.Fine root systems had the most pivotal influence on soil preferential flow in this forest ecosystem.In all experimental plots,RB content was the sum of RB from soil preferential pathways and the soil matrix in each soil depth.With respect to 6 soil depth gradient（0–10,10–20,20–30,30–40,40–50,50–60 cm） in each plot,the number of soil depth gradient that RB content was greater in soil preferential pathways than in the soil matrix was characterized,and the proportion was68.2 % in all plots.
基金supported by the earmarked fund for the China Agriculture Research System (CARS-27)
文摘Soil alkalinity is a major factor that restricts the growth of apple roots.To analyze the response of apple roots to alkali stress, the root structure and endogenous hormones of two apple rootstocks, Malus prunifolia (alkali-tolerant) and Malus hupehensis (alkali-sensitive), were compared. To understand alkali tolerance of M. prunifolia at the molecular level, transcriptome analysis was performed. When plants were cultured in alkaline conditions for 15 d, the root growth of M. hupehensis with weak alkali tolerance decreased significantly. Analysis of endogenous hormone levels showed that the concentrations of indole-3-acetic acid (IAA) and zeatin riboside (ZR) in M. hupehensis under alkali stress were lower than those in the control. However, the trend for IAA and ZR in M. prunifolia was the opposite. The concentration of abscisic acid (ABA) in the roots of the two apple rootstocks under alkali stress increased, but the concentration of ABA in the roots of M. prunifolia was higher than that in M. hupehensis. The expression of IAA-related genes ARF5, GH3.6, SAUR36, and SAUR32 and the Cytokinin (CTK)-related gene IPT5 in M. prunifolia was higher than those in the control, but the expression of these genes in M. hupehensis was lower than those in the control. The expression of ABA-related genes CIPK1 and AHK1 increased in the two apple rootstocks under alkali stress, but the expression of CIPK1 and AHK1 in M. prunifolia was higher than in M. hupehensis. These results demonstrated that under alkali stress, the increase of IAA, ZR, and ABA in roots and the increase of the expression of related genes promoted the growth of roots and improved the alkali tolerance of apple rootstocks.
基金funded by National Natural Science Foundation of China (Grant No.32072575)Postgraduate Research & Practice Innovation Program of Jiangsu Province (Grant No.KYCX20_0588)National Vegetable Industry Technology System (Grant No.CARS-23-A16)。
文摘Non-heading Chinese cabbage, a variety of Brassica campestris, is an important vegetable crop in the Yangtze River Basin of China. However,the immaturity of its stable transformation system and its low transformation efficiency limit gene function research on non-heading Chinese cabbage. Agrobacterium rhizogenes-mediated(ARM) transgenic technology is a rapid and effective transformation method that has not yet been established for non-heading Chinese cabbage plants. Here, we optimized conventional ARM approaches(one-step and two-step transformation methods) suitable for living non-heading Chinese cabbage plants in nonsterile environments. Transgenic roots in composite non-heading Chinese cabbage plants were identified using phenotypic detection, fluorescence observation, and PCR analysis. The transformation efficiency of a two-step method on four five-day-old non-heading Chinese cabbage seedlings(Suzhouqing, Huangmeigui, Wuyueman, and Sijiu Caixin) was 43.33%-51.09%, whereas using the stout hypocotyl resulted in a transformation efficiency of 54.88% for the 30-day-old Sijiu Caixin.The one-step method outperformed the two-step method;the transformation efficiency of different varieties was above 60%, and both methods can be used to obtain transgenic roots for functional studies within one month. Finally, optimized ARM transformation methods can easily,quickly, and effectively produce composite non-heading Chinese cabbage plants with transgenic roots, providing a reliable foundation for gene function research and non-heading Chinese cabbage genetic improvement breeding.
基金Supported by University Research Project of Education Dpartment(2018A-035)~~
文摘[Objective] Iron deficiency is one of the most important crop element deficiencies in the Loess Plateau of northwestern China. The selection for crop cultivars that are tolerant to low iron levels could be one of the approaches to solving the problem and improving crop production. [Method] Three major apple root stock species （Malus prunifolia, Malus sieversii and Malus baccata） were selected to evaluate their tolerance to iron defciency in hydroponic system. A 3×2 factorial pot experiment was conducted with three replicates in a greenhouse at Gansu Agricultural University, Lanzhou, China. [Result] The SOD, POD and CAT activities in roots and stems of the 3 root stock species in Fe-defcient Hoagland solution decreased, however Malus sieversii got the less reduction and had better root architecture and growth than the other species. The aboveground biomass, plant height, chlorophyll content, total root length and lateral root number were correlated positively with iron-defciency stress tolerance. The species’ tolerance to iron-defciency from high to low was M. sieversii’s〉M. baccata’s〉M. prunifolia’s. Moreover, the improvement of some morphological features such as root length, above-ground biomass, plant height and lateral root number in apple could be conducive to breeding cultivars with tolerance to iron-defciency stress. [Conclusion] Malus sieversii had better tolerance to iron-defciency stress than the others in this study.
文摘Based on the survey of 154 farmers in Guiyang City, we analyze the basic situation of building of grass-roots agricultural technology extension system in Guiyang City. Then we point out some problems in the building of grass-roots agricultural technology extension system in Guiyang City: the function of grass-roots agricultural technology extension center weakens; the content of agricultural technology extension is difficult to adapt to farmers' needs for technology; the extension mode of agricultural technology departments does not adapt to the needs of modern agriculture. In order to perfect the building of grass-roots agricultural technology extension system, the countermeasures and recommendations are put forth as follows: strengthening the input of funds, and ensuring that the basic work of public welfare agricultural technology extension is smoothly carried out; innovating upon the system, and improving the function of grass-roots agricultural technology extension center; implementing management on agricultural technology extension personnel's performance, and promoting the extension efficiency; strengthening the building of extension team in rural areas, and cultivating high-quality agricultural technology extension personnel; exploring the advanced service mode to meet farmers' needs.
基金The research was supported by the Science Fund of China Post Ph.D （2003034492）Science Foundation of Chongqing Educational Committee （040216）, China.
文摘Genetic transformation experiments of the different explants from Citrus grandis cv. Changshou Shatian You infected with Agrobacterium rhizogenes were carried out in darkness or in light. The optimizing culture system of Ri T-DNA transformed roots for C. grandis cv. Changshou Shatian You was constructed as follows： After the ventral wounded striations on the single activation cotyledon were inoculated by A. rhizogenes A4 （logarithmic period）, they were cocultured at （25 ±2）℃ in darkness for 25-30 days; some transformed roots were generated from wounded striations of most cotyledons. The genetically transformed ratio is （83 ± 11）%. Axenic Ri T-DNA transformed roots （hairy roots） were harvested after five subcultures. Explants were activated on MT medium. The MS medium was used for subculture of transformed roots. Mass Ri T-DNA transformed roots in which the hormone was produced independently were harvested from this optimizing culture system. White, fresh Ri T-DNA transformed roots were （1.14 ±0.07） cm long, （0.73 ±0.04） mm wide, and the growth direction of transformed roots was negative geotropism.
基金supported by the National Natural Science Foundation of China(31600479,32371576)the Key R&D Program of Yunnan Province(202403AC100028)+1 种基金the Youth Academic and Technical Leading Talent Reserve Program in Yunnan Province(202405AC350012)the 14th Five-Year Plans of Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences(XTBG-1450101,E3ZKFF2B01).
文摘Climate change has significantly increased the frequency and severity of droughts and risk of tree death worldwide.Differences in leaf habit,plant size,and species diversity are associated with differences in the risk of drought-induced mortality,but the relative contributions of these factors to the risk of mortality are unclear.In a study of the mortality of tree and shrub species during the extreme drought of 2019 in a savanna ecosystem in Southwest China,we assessed the relative contributions of evergreen and deciduous leaf habit,plant size,and species richness and diversity to the mortality of shrubs and trees after the 2019 extreme drought.The deciduous species had significantly lower hydraulic safety margins than the coexisting evergreen species,resulting in a higher mortality risk.Additionally,species and individuals with taller canopies tended to have deeper root systems,an advantage during extreme drought that reduced mortality risk.Notably,mortality risk was largely independent of stand species richness and diversity.Overall,leaf habit and plant height were better predictors of mortality risk than species richness and diversity.These novel insights provide a better understanding of the mechanisms driving drought-induced mortality in the ecosystems with a low canopy and weak interspecific and intraspecific competition for shared resources.Leaf habit and tree size should be incorporated into hypotheses on the mechanisms underlying drought-induced tree mortality.
基金supported by the Qinghai Science and Technology Department Project(2025-QY-225)the National Natural Science Foundation of China(42267024)the Second Comprehensive Scientific Investigation and Research Project of the Qinghai-Xizang Plateau(2019QZKK0905).
文摘The Qinghai-Xizang Plateau of China faces challenges like thaw slumping,threatening slope stability and infrastructure.Understanding the mechanical properties of the roots of the dominant herbaceous plant species in the alpine meadow layer of the permafrost regions on the Qinghai-Xizang Plateau is essential for evaluating their role in enhancing soil shear strength and mitigating slope deformation in these fragile environments.In this study,the roots of four dominant herbaceous plant species—Kobresia pygmaea,Kobresia humilis,Carex moorcroftii,and Leontopodium pusillum—that are widely distributed in the permafrost regions of the Qinghai-Xizang Plateau were explored to determine their mechanical properties and effects in enhancing soil shear strength.Through indoor single root tensile and root group tensile tests,we determined the root diameter,tensile force,tensile strength,tensile ratio,and strength frequency distributions.We also evaluated their contributions to inhibiting slope deformation and failure during the formation and development of thermal thaw slumps in the alpine meadow.The results showed that the distribution of the root diameter of the dominant plant species is mostly normal,while the tensile strength tends to be logarithmically normally distributed.The relationship between the root diameter and root tensile strength conforms to a power function.The theoretical tensile strength of the root group was calculated using the Wu-Waldron Model(WWM)and the Fiber Bundle Model(FBM)under the assumption that the cumulative single tensile strength of the root bundle is identical to the tensile strength of the root group in the WWM.The FBM considers three fracture modes:FBM-D(the tensile force on each single root is proportional to its diameter relative to the total sum of all the root diameters),FBM-S(the cross-sectional stress in the root bundle is uniform),and FBM-N(each tensile strength test of individual roots experiences an equal load).It was found that the model-calculated tensile strength of the root group was 162.60%higher than the test value.The model-derived tensile force of the root group from the FBM-D,FBM-S,and FBM-N was 73.10%,28.91%,and 13.47%higher than the test values,respectively.The additional cohesion of the soil provided by the roots was calculated to be 25.90-45.06 kPa using the modified WWM,67.05-38.15 kPa using the FBM-S,and 57.24-32.74 kPa using the FBM-N.These results not only provide a theoretical basis for further quantitative evaluation of the mechanical effects of the root systems of herbaceous plant species in reinforcing the surface soil but also have practical significance for the effective prevention and control of thermal thaw slumping disasters in the permafrost regions containing native alpine meadows on the Qinghai-Xizang Plateau using flexible plant protection measures.
基金supported by Natural Science Foundation of Chongqing(No.CSTB2022NSCQ-LZX0001)High-end Foreign Expert Introduction program(No.G2022165004L)+1 种基金High-end Foreign Expert Introduction program(No.DL2021165001L)The fi-nancial supports are gratefully acknowledged.
文摘Plant root systems,a crucial component of biogeotechnics,have been recognized as a promising and sustainable strategy to address novel challenges in geotechnical engineering,i.e.,climate change(Ng et al.,2022).Root-soil composite and root-reinforced slopes have re-ceived widespread attention in recent decades,due to the ability of root to regulate soil properties through mechanical reinforcement and hy-draulic transpiration(Li&Duan,2023;Ni et al.,2024).Fig.1 provides a co-occurrence network plot of plant root-based soil reinforcement strategies published over the last decade,where three clusters are identified with different colors.On the left of the network map,clusters in red and blue are primarily driven by geotechnical investigations of vegetated slopes(i.e.,plant root reinforced slopes)and root-soil com-posite/root-permeated soils,as denoted by the terms like"model","test","slope","strength"and"vegetation",while the green cluster on the right side demonstrates botany-related domains,for instance,"plant growth",Indeed,the reinforcement of vegetated soil strength is com-plex and varies significantly with an abundance of factors,both me-chanically and hydraulically.Particularly,the impact of root mor-phology and architecture cannot be negligible,including keywords"root area ratio"root distribution""root morphology"root diame-ter"root density"in Fig.1 with the root size and root depth ranking foremost.
文摘The article contains a list of 14 structured technical sheets on roots, rhizomes or tubers that will serve as guidance in improving the quality of health of people. The used method was through an active search for literature that brought the observed aspects to the structuring of the technical sheets, i.e., literature that contains data related to composition, active ingredients, interaction (when described in the consulted literature), health condition in which they may be useful in helath care by various health professionals and anyone who may be of interest. The data obtained shows that there is a need for a careful evaluation of the foods (that contain roots, rhizomes, or tubers) used by polymedicated people due to possible interactions and incompatibilities that may exist.
文摘Root resorption is a significant complication in orthodontic treatment,with thin roots and reciprocal movement being recognized as high-risk factors.This paper reports a case of a 19-year-old female patient who underwent orthodontic treatment for dental irregularity.The patient had thin roots in the maxillary lateral incisors 12 and 22.During treatment,tooth 22 experienced reciprocal movement of labial expansion followed by retraction,while tooth 12 adopted passive ligation to reduce reciprocal movement.After 23 months of straight-wire extraction treatment,good occlusal relationships were achieved,but significant root resorption occurred in teeth 12 and 22,with tooth 22 showing more severe resorption.This case confirms the synergistic effect between thin roots and reciprocal movement,demonstrating that thin roots are more sensitive to reciprocal movement stimulation,producing a synergistic amplification effect.Additionally,standardized nursing guidance and patient compliance management play important roles in reducing resorption risk.This case emphasizes the importance of pretreatment risk assessment,individualized treatment strategy formulation,and comprehensive nursing intervention throughout treatment,providing reference for clinical prevention of root resorption.
基金funded by the Guangxi Science and Technology Plan Project(Guike AD22080050)the Basic Research Ability Improvement Project of Young and Middle-aged Teachers of Universities in Guangxi(2022KY0386)+1 种基金the Opening Foundation of Key Laboratory of Environment Change and Resources Use in Beibu Gulf,Ministry of Education,Nanning Normal University(NNNU-KLOP-K2202)the National Natural Science Foundation of China(42471055).
文摘Root system architecture has often been overlooked in plant research despite its critical role in plant adaptation to environmental conditions.This study focused on the root system architecture of the desert shrub Reaumuria soongorica in the Alxa steppe desert,Northwest China.Plant samples were collected during May-September 2019.Using excavation methods,in situ measurements,and root scanning techniques,we analyzed the root distribution,topology,and branching patterns of R.soongorica across an age sequence of 7-51 a.Additionally,we investigated the allometric relationships of root collar diameter with total coarse root length,biomass,and topological parameters.The results showed that the roots of R.soongorica were predominantly concentrated in shallow soil layers(10-50 cm),with lateral root branching and biomass allocation increasing with shrub age.The root topology exhibited a herringbone-like structure,with average topological and modified topological indices of 0.89 and 0.96,respectively,both of which adjusted with shrub age.The root system displayed a self-similar branching pattern,maintaining a constant cross-sectional area ratio of 1.13 before and after branching,deviating from the area-preserving rule.These adaptive traits allow R.soongorica to efficiently expand its nutrient acquisition zone,minimize internal competition,and optimize resource uptake from the upper soil layers.Furthermore,significant linear relationships were observed between log10-transformed root collar diameter and log10-transformed total coarse root length,biomass,and topological parameters.These findings advance non-destructive approaches for studying root characteristics and contribute to the development of root-related models.Besides,this study provides new insights into the adaptive strategies of R.soongorica under extreme drought conditions,offering valuable guidance for species selection and cultivation in desert restoration efforts.
文摘Two decades ago,an important development philosophy emerged that would redefine prosperity:“Lucid waters and lush mountains are invaluable assets.”Known as the“Two Mountains”theory,it challenged the conventional trade-off between development and the environment by arguing that they are inseparable.
文摘BACKGROUND The numbers of mandibular first premolar roots and root canals vary,and the incidence of three roots and three canals is 0.09%.CASE SUMMARY In this article,we review the root and root canal conditions for the mandibular first premolar and report the case of a mandibular left first premolar with three roots and three canals in a male patient,with suggestions for clinical diagnosis and treatment.The patient was referred by an orthodontist for the extraction of the tooth.Preoperative cone-beam computed tomography examination revealed that it had three roots.Under local anesthesia,the extraction socket was carefully expanded,and the tooth was successfully removed intact using forceps.The procedure was uneventful,with no root fractures,postoperative bleeding,or sensory abnormality observed.CONCLUSION The mandibular first premolar is characterized by multiple roots and canal variations that can increase the difficulty of treatment.
文摘As French Tibetologist Alexandra David-Neel noted,"For many centuries the history of Xizang has been inseparable from that of China."①In the long course of China's five-thousand-year history,Xizang and the Central Plains have,through economic exchanges,political interactions and cultural contacts,developed a strong bond.These historically rooted close links are a reflection of the intermingled distribution,cultural inclusiveness.
基金supported by the projects of the National Key Research and Development Program of China(2024YFD2301305)Jiangsu Innovation Support Program for International Science and Technology Cooperation Project(BZ2023049)+2 种基金the projects of the National Natural Science Foundation of China(32272213)the China Agriculture Research System(CARS-03)Jiangsu Collaborative Innovation Center for Modern Crop Production(JCIC-MCP).
文摘Optimizing root system architecture(RSA)is essential for plants because of its critical role in acquiring water and nutrients from the soil.However,the subterranean nature of roots complicates the measurement of RSA traits.Recently developed rhizobox methods allow for the rapid acquisition of root images.Nevertheless,effective and precise approaches for extracting RSA features from these images remain underdeveloped.Deep learning(DL)technology can enhance image segmentation and facilitate RSA trait extraction.However,comprehensive pipelines that integrate DL technologies into image-based root phenotyping techniques are still scarce,hampering their implementation.To address this challenge,we present a reproducible pipeline(faCRSA)for automated RSA traits analysis,consisting of three modules:(1)the RSA traits extraction module functions to segment soil-root images and calculate RSA traits.A lightweight convolutional neural network(CNN)named RootSeg was proposed for efficient and accurate segmentation;(2)the data storage module,which stores image and text data from other modules;and(3)the web application module,which allows researchers to analyze data online in a user-friendly manner.The correlation coefficients(R^(2))of total root length,root surface area,and root volume calculated from faCRSA and manually measured results were 0.96**,0.97**,and 0.93**,respectively,with root mean square errors(RMSE)of 8.13 cm,1.68 cm^(2),and 0.05 cm^(3),processed at a rate of 9.74 s per image,indicating satisfying accuracy.faCRSA has also demonstrated satisfactory performance in dynamically monitoring root system changes under various stress conditions,such as drought or waterlogging.The detailed code and deployable package of faCRSA are provided for researchers with the potential to replace manual and semi-automated methods.
文摘INTRODUCTION Cardiovascular system involvement is an important determinant of long-term prognosis in patients with systemic lupus erythematosus(SLE).Aneurysmal dilatation of the aortic root combined with Stanford type A aortic dissection(TAAD)is a highly catastrophic complication in these patients.
