Protoplasts of Page tangelo (Citrus reticulata Blanco×C. paradisi Macf.) cell suspension culture were electrically fused with mesophyll protoplasts isolated from trifoliate orange (Poncirus trifoliata (L.) Raf.)....Protoplasts of Page tangelo (Citrus reticulata Blanco×C. paradisi Macf.) cell suspension culture were electrically fused with mesophyll protoplasts isolated from trifoliate orange (Poncirus trifoliata (L.) Raf.). More than 150 plantlets regenerated after 4-5 months of culture. The regenerated plants were trifoliate with well developed root systems. Root_tip chromosome counting of more than 20 randomly selected plants revealed that they were all tetraploids (2n=4x=36). RAPD analysis of 7 randomly selected plants verified their hybridity. Inoculation of citrus Phytophthora parasitica Dastar toxin on leaves of somatic hybrids and both parental genotypes showed that Page tangelo was moderately susceptible, and trifoliate orange was highly resistant while the somatic hybrids were resistant. The potential of this somatic hybrid as rootstock is also discussed.展开更多
The bushy root-2(brt-2)tomato mutant has twisting roots,and slower plant development.Here we used whole genome resequencing and genetic mapping to show that brt-2 is caused by a serine to cysteine(S75C)substitution in...The bushy root-2(brt-2)tomato mutant has twisting roots,and slower plant development.Here we used whole genome resequencing and genetic mapping to show that brt-2 is caused by a serine to cysteine(S75C)substitution in the DNA binding domain(DBD)of a heat shock factor class B(HsfB)encoded by SolycHsfB4a.This gene is orthologous to the Arabidopsis SCHIZORIZA gene,also known as AtHsfB4.The brt-2 phenotype is very similar to Arabidopsis lines in which the function of AtHsfB4 is altered:a proliferation of lateral root cap and root meristematic tissues,and a tendency for lateral root cap cells to easily separate.The brt-2 S75C mutation is unusual because all other reported amino acid substitutions in the highly conserved DBD of eukaryotic heat shock factors are dominant negative mutations,but brt-2 is recessive.We further show through reciprocal grafting that brt-2 exerts its effects predominantly through the root genotype even through BRT-2 is expressed at similar levels in both root and shoot meristems.Since AtHsfB4 is induced by root knot nematodes(RKN),and loss-of-function mutants of this gene are resistant to RKNs,BRT-2 could be a target gene for RKN resistance,an important trait in tomato rootstock breeding.Gene&accession numbers SolycHsfB4a-Solyc04g078770.展开更多
文摘Protoplasts of Page tangelo (Citrus reticulata Blanco×C. paradisi Macf.) cell suspension culture were electrically fused with mesophyll protoplasts isolated from trifoliate orange (Poncirus trifoliata (L.) Raf.). More than 150 plantlets regenerated after 4-5 months of culture. The regenerated plants were trifoliate with well developed root systems. Root_tip chromosome counting of more than 20 randomly selected plants revealed that they were all tetraploids (2n=4x=36). RAPD analysis of 7 randomly selected plants verified their hybridity. Inoculation of citrus Phytophthora parasitica Dastar toxin on leaves of somatic hybrids and both parental genotypes showed that Page tangelo was moderately susceptible, and trifoliate orange was highly resistant while the somatic hybrids were resistant. The potential of this somatic hybrid as rootstock is also discussed.
基金The research was supported by BBSRC-UKRI fundingthe RootLINK(BB/L01954X/1)project focused on the“Understanding the Genetic Basis of Traits for Rootstock Improvement in Vegetable Crops”.
文摘The bushy root-2(brt-2)tomato mutant has twisting roots,and slower plant development.Here we used whole genome resequencing and genetic mapping to show that brt-2 is caused by a serine to cysteine(S75C)substitution in the DNA binding domain(DBD)of a heat shock factor class B(HsfB)encoded by SolycHsfB4a.This gene is orthologous to the Arabidopsis SCHIZORIZA gene,also known as AtHsfB4.The brt-2 phenotype is very similar to Arabidopsis lines in which the function of AtHsfB4 is altered:a proliferation of lateral root cap and root meristematic tissues,and a tendency for lateral root cap cells to easily separate.The brt-2 S75C mutation is unusual because all other reported amino acid substitutions in the highly conserved DBD of eukaryotic heat shock factors are dominant negative mutations,but brt-2 is recessive.We further show through reciprocal grafting that brt-2 exerts its effects predominantly through the root genotype even through BRT-2 is expressed at similar levels in both root and shoot meristems.Since AtHsfB4 is induced by root knot nematodes(RKN),and loss-of-function mutants of this gene are resistant to RKNs,BRT-2 could be a target gene for RKN resistance,an important trait in tomato rootstock breeding.Gene&accession numbers SolycHsfB4a-Solyc04g078770.
