Vibration behaviors of bogie hunting motion contain key information that dominates the dynamic performance of rail vehicles,in which the eigenvalue of each mode reflects the damping ratio and the natural frequency.Thi...Vibration behaviors of bogie hunting motion contain key information that dominates the dynamic performance of rail vehicles,in which the eigenvalue of each mode reflects the damping ratio and the natural frequency.This paper focuses on the root loci curves of bogie hunting motion,starting from a rigid bogie,then to a bogie with flexible primary suspension.With regard to the rigid bogie,analytical formulas for the eigenvalues,the critical speed as well as the corresponding hunting frequency are derived and verified.While for the flexible bogie,the root loci curves are calculated numerically.The study shows that both free rigid bogie and free wheelset are dynamically unstable at any speed.The critical speed increases with diminished wheel-rail conicity,track gauge,and wheelset and bogie inertia,and with increased wheelbase and wheel radius.The dominating factors such as the stiffness of the primary suspension and the wheel-rail conicity should be optimized for a practical design.The influences of the damping coefficients and the variations of creep coefficients are negligible.The motor suspension affects the root loci curves and the critical speed significantly.Both inappropriate motor suspension design and rigidly suspended motor reduce the critical speed.The increase of critical speed by a motor suspension can only be achieved when the lower natural frequency of the motor-bogie frame-wheelsets system coincides with or is close to the hunting frequency.Special care should be taken for the design of motor suspension,the first is to avoid the decreased damping ratio in a certain speed range below the critical speed and the second is that the variations of parameters should not induce the rapid reduction of the critical speed.The main feature of the present study is that the root loci curves,which are derived as analytical formulas or calculated numerically,are used to study the vibrational behaviors of bogie hunting motion.Both the influencing laws of the dominating parameters and the principles regarding the motor suspension are significant for the stability design of modem railway vehicles which may use innovative structures/materials as well as modem control and monitoring technologies.展开更多
Root system architecture (RSA) plays an important role in phosphorus (P) acquisition, but enhancing P use efficiency (PUE) in maize via genetic manipulation of RSA has not yet been reported. Here, using a maize ...Root system architecture (RSA) plays an important role in phosphorus (P) acquisition, but enhancing P use efficiency (PUE) in maize via genetic manipulation of RSA has not yet been reported. Here, using a maize recombinant inbred line (RIL) population, we investigated the genetic relationships between PUE and RSA, and developed P-efficient lines by selection of quantitative trait loci (QTLs) that coincide for both traits. In low-P (LP) fields, P uptake efficiency (PupE) was more closely correlated with PUE (r = 0.48 -0.54), and RSA in hydroponics was significantly related to PupE (r=0.25-0.30) but not to P utilization efficiency (PutE). QTL analysis detected a chromosome region where two QTLs for PUE, three for PupE and three for RSA were assigned into two QTL clusters, Cl-bin3.04a and Cl-bin3.04b. These QTLs had favorable effects from alleles derived from the large-rooted and high-PupE parent. Marker-assisted selection (MAS) identified nine advanced backcross-derived lines carrying Cl-bin3.04a or Cl-bin3.04b that displayed mean increases of 22%-26% in PUE in LP fields. Furthermore, a line L224 pyramiding Cl- binB.04a and Cl-bin3.04b showed enhanced PupE, relying mainly on changes in root morphology, rather than root physiology, under both hydroponic and field conditions. These results highlight the physiological and genetic contributions of RSA to maize PupE, and provide a successful study case of developing P-efficient crops through QTL-based selection.展开更多
Introgression lines (ILs) are valuable materials for identifying quantitative trait loci (QTLs), evaluating genetic interactions, and marker assisted breeding. A set of 430 ILs (BC5F3) containing segments from u...Introgression lines (ILs) are valuable materials for identifying quantitative trait loci (QTLs), evaluating genetic interactions, and marker assisted breeding. A set of 430 ILs (BC5F3) containing segments from upland tropical japonica cultivar IRAT109 in a lowland temperate japonica cultivar Yuefu background were developed. One hundred and seventy-six polymorphic markers were used to identify introgressed segments. No segment from IRAT109 was found in 160 lines. Introgressed segments of the other 270 lines covered 99.1% of the donor genome. The mean number of introgressed donor segments per individual was 3.3 with an average length of 14.4 cM. QTL analysis was conducted on basal root thickness (BRT) of the 270 ILs grown under irrigated lowland, upland and hydroponic conditions. A total of 22 QTLs affecting BRT were identified, six QTLs (qBRT3.1, qBRT3.2, qBRT6.1, qBRTS.2, qBRT9.1, and qBRT9.2) were consistently expressed under at least two environments (location and water regime), and qBRT7.2 was a new BRT QTL identified under lowland conditions. IL255 containing qBRT9.1 showed an increase of 10.09% and 7.07% BRT over cultivar Yuefu when grown under upland and lowland conditions, respectively. Using a population of 304 F2:3 lines derived from the cross IL255 Yuefu, qBRT9.1 was validated and mapped to a 1.2 cM interval between RM24271 and RM566. The presence of qBRT9.1 explained 12% of BRT variation. The results provide upland rice ILs and BRT QTLs for analyzing the genetic basis of drought resistance, detecting favorable genes from upland rice, and rice drought resistance breeding.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.51805452,and 51935002)the Independent Research Project of State Key Laboratory of Traction Power(Grant No.2020TPL-T02).
文摘Vibration behaviors of bogie hunting motion contain key information that dominates the dynamic performance of rail vehicles,in which the eigenvalue of each mode reflects the damping ratio and the natural frequency.This paper focuses on the root loci curves of bogie hunting motion,starting from a rigid bogie,then to a bogie with flexible primary suspension.With regard to the rigid bogie,analytical formulas for the eigenvalues,the critical speed as well as the corresponding hunting frequency are derived and verified.While for the flexible bogie,the root loci curves are calculated numerically.The study shows that both free rigid bogie and free wheelset are dynamically unstable at any speed.The critical speed increases with diminished wheel-rail conicity,track gauge,and wheelset and bogie inertia,and with increased wheelbase and wheel radius.The dominating factors such as the stiffness of the primary suspension and the wheel-rail conicity should be optimized for a practical design.The influences of the damping coefficients and the variations of creep coefficients are negligible.The motor suspension affects the root loci curves and the critical speed significantly.Both inappropriate motor suspension design and rigidly suspended motor reduce the critical speed.The increase of critical speed by a motor suspension can only be achieved when the lower natural frequency of the motor-bogie frame-wheelsets system coincides with or is close to the hunting frequency.Special care should be taken for the design of motor suspension,the first is to avoid the decreased damping ratio in a certain speed range below the critical speed and the second is that the variations of parameters should not induce the rapid reduction of the critical speed.The main feature of the present study is that the root loci curves,which are derived as analytical formulas or calculated numerically,are used to study the vibrational behaviors of bogie hunting motion.Both the influencing laws of the dominating parameters and the principles regarding the motor suspension are significant for the stability design of modem railway vehicles which may use innovative structures/materials as well as modem control and monitoring technologies.
基金financially supported by the National Key Research and Development Program of China(No.2016YFD0100700)the National Science Foundation of China(Nos.31572186 and 31421092)
文摘Root system architecture (RSA) plays an important role in phosphorus (P) acquisition, but enhancing P use efficiency (PUE) in maize via genetic manipulation of RSA has not yet been reported. Here, using a maize recombinant inbred line (RIL) population, we investigated the genetic relationships between PUE and RSA, and developed P-efficient lines by selection of quantitative trait loci (QTLs) that coincide for both traits. In low-P (LP) fields, P uptake efficiency (PupE) was more closely correlated with PUE (r = 0.48 -0.54), and RSA in hydroponics was significantly related to PupE (r=0.25-0.30) but not to P utilization efficiency (PutE). QTL analysis detected a chromosome region where two QTLs for PUE, three for PupE and three for RSA were assigned into two QTL clusters, Cl-bin3.04a and Cl-bin3.04b. These QTLs had favorable effects from alleles derived from the large-rooted and high-PupE parent. Marker-assisted selection (MAS) identified nine advanced backcross-derived lines carrying Cl-bin3.04a or Cl-bin3.04b that displayed mean increases of 22%-26% in PUE in LP fields. Furthermore, a line L224 pyramiding Cl- binB.04a and Cl-bin3.04b showed enhanced PupE, relying mainly on changes in root morphology, rather than root physiology, under both hydroponic and field conditions. These results highlight the physiological and genetic contributions of RSA to maize PupE, and provide a successful study case of developing P-efficient crops through QTL-based selection.
基金funded by the National Basic Research Program of China(No.2010CB1125904)the National Key Technologies R&D Program of China(No.2009BADA2B01)+2 种基金the National Natural Science Foundation of China(No. 31061140458)"948"Project of MOA(No.2011-G2B)the Key Project of Transgenic Crop Improvement of China (No.2009ZX08009-073B)
文摘Introgression lines (ILs) are valuable materials for identifying quantitative trait loci (QTLs), evaluating genetic interactions, and marker assisted breeding. A set of 430 ILs (BC5F3) containing segments from upland tropical japonica cultivar IRAT109 in a lowland temperate japonica cultivar Yuefu background were developed. One hundred and seventy-six polymorphic markers were used to identify introgressed segments. No segment from IRAT109 was found in 160 lines. Introgressed segments of the other 270 lines covered 99.1% of the donor genome. The mean number of introgressed donor segments per individual was 3.3 with an average length of 14.4 cM. QTL analysis was conducted on basal root thickness (BRT) of the 270 ILs grown under irrigated lowland, upland and hydroponic conditions. A total of 22 QTLs affecting BRT were identified, six QTLs (qBRT3.1, qBRT3.2, qBRT6.1, qBRTS.2, qBRT9.1, and qBRT9.2) were consistently expressed under at least two environments (location and water regime), and qBRT7.2 was a new BRT QTL identified under lowland conditions. IL255 containing qBRT9.1 showed an increase of 10.09% and 7.07% BRT over cultivar Yuefu when grown under upland and lowland conditions, respectively. Using a population of 304 F2:3 lines derived from the cross IL255 Yuefu, qBRT9.1 was validated and mapped to a 1.2 cM interval between RM24271 and RM566. The presence of qBRT9.1 explained 12% of BRT variation. The results provide upland rice ILs and BRT QTLs for analyzing the genetic basis of drought resistance, detecting favorable genes from upland rice, and rice drought resistance breeding.