[Objective] The aim was to explore biomass and growth of Cupressus lusitanica seedlings in order to provide technological supports for afforestation. [Method] The introduced Cupressus lusitanica seedlings at different...[Objective] The aim was to explore biomass and growth of Cupressus lusitanica seedlings in order to provide technological supports for afforestation. [Method] The introduced Cupressus lusitanica seedlings at different ages were re- searched to explore biomass （ground or underground） morphological shape, surface area and volume of a root system. [Result] As age grew, plant height and ground diameter increased significantly, varying dramatically upon seed provenance, and root-canopy ratio, root length, surface area and volume of seedlings were increas- ing. Specifically, seedlings at two-year-old and three-year-old kept a stable growth; moderate and big roots took higher proportions in a root system; underground parts for a plant required more photosynthetic products; root systems of young seedlings grew to moderate and big roots. [Conclusion] Ground biomass is of correlation with root system indices and the morphological characteristics of root systems are close to biomass. The accumulation capacity of biomass differed for seedlings from differ- ent provenances, even in the same environment and at the same time.展开更多
This study examined the isolation and differentiation of dorsal root ganglion progenitor cells for therapeutic use in neurodegenerative diseases. Rat embryonic dorsal root ganglia progenitors were isolated and purifie...This study examined the isolation and differentiation of dorsal root ganglion progenitor cells for therapeutic use in neurodegenerative diseases. Rat embryonic dorsal root ganglia progenitors were isolated and purified using the differential adhesion method combined with cytosine arabinoside treatment. After culture in serum-free medium supplemented with B27, basic fibroblast growth factor and epidermal growth factor, these cells remained viable and survived for more than 18 months in vitro. Most cells differentiated to neurons that were immunoreactive for gamma-aminobutyric acid and choline acetyltransferase as detected by immunohistochemical staining. In addition, nerve growth factor and neurotrophic tyrosine kinase receptor expression were also observed in dorsal root ganglion progenitors and differentiated cells. K252a, an inhibitor that blocks nerve growth factor-induced signaling, inhibited cell survival, suggesting the possible existence of a nerve growth factor autocrine loop in these proliferating cells.展开更多
基金Supported by National Program "948"(2009-4-17)~~
文摘[Objective] The aim was to explore biomass and growth of Cupressus lusitanica seedlings in order to provide technological supports for afforestation. [Method] The introduced Cupressus lusitanica seedlings at different ages were re- searched to explore biomass （ground or underground） morphological shape, surface area and volume of a root system. [Result] As age grew, plant height and ground diameter increased significantly, varying dramatically upon seed provenance, and root-canopy ratio, root length, surface area and volume of seedlings were increas- ing. Specifically, seedlings at two-year-old and three-year-old kept a stable growth; moderate and big roots took higher proportions in a root system; underground parts for a plant required more photosynthetic products; root systems of young seedlings grew to moderate and big roots. [Conclusion] Ground biomass is of correlation with root system indices and the morphological characteristics of root systems are close to biomass. The accumulation capacity of biomass differed for seedlings from differ- ent provenances, even in the same environment and at the same time.
基金supported by the Rhone Alps Region of France, MIRA grants for the collaboration,the Agence National de la Recherche (ANR) RIB,the CNRS (International program for Scientific Cooperation, "PICS")
文摘This study examined the isolation and differentiation of dorsal root ganglion progenitor cells for therapeutic use in neurodegenerative diseases. Rat embryonic dorsal root ganglia progenitors were isolated and purified using the differential adhesion method combined with cytosine arabinoside treatment. After culture in serum-free medium supplemented with B27, basic fibroblast growth factor and epidermal growth factor, these cells remained viable and survived for more than 18 months in vitro. Most cells differentiated to neurons that were immunoreactive for gamma-aminobutyric acid and choline acetyltransferase as detected by immunohistochemical staining. In addition, nerve growth factor and neurotrophic tyrosine kinase receptor expression were also observed in dorsal root ganglion progenitors and differentiated cells. K252a, an inhibitor that blocks nerve growth factor-induced signaling, inhibited cell survival, suggesting the possible existence of a nerve growth factor autocrine loop in these proliferating cells.
