To study the influence of photoperiod on roots differentiation in the Tunisian grapevine (Vitis vinifera L.) cultivar Perle noir, roots and callus initiation were analyzed under three different conditions of day lengt...To study the influence of photoperiod on roots differentiation in the Tunisian grapevine (Vitis vinifera L.) cultivar Perle noir, roots and callus initiation were analyzed under three different conditions of day length: long day (LD), short day (SD) and darkness (D). The photoperiod influenced the number of callus and roots per cuttings;it has a significant effect on the roots and callus initiation. Expression profile analysis of six MADS-box genes (VTM8, VSEP2, VAG12, VAG17-1, VAG17-2 and VSOC1.3) during root and callus development is in agreement with the above-mentioned observation. The expression of the MADS-box genes during root and callus development fluctuated in a tissue-dependent manner. These data suggest that all genes are expressed in roots under three photoperiods. Total darkness gives the number of the most important root per cutting compared to the other two conditions. This photoperiodic condition gave the most important expression of the studied genes VAG12, VAG17-2, VAG17-1, VTM8 and VSEP2 transcripts were not found in callus grown in the dark or in LD conditions, respectively. VSOC1.3 transcripts were not found in callus grown in the dark or in SD conditions, respectively. Transcript abundance of VTM8 and VSOC1 was highest in LD.展开更多
F-box蛋白作为SCF(Skp1,Cullin and an F-box protein)复合体的成员,参与调节植物的生长发育过程。At5g22700为功能未知的F-box基因家族成员。本研究通过酵母双杂交分析At5g22700蛋白与ASK(Arabidopsis-SKP1-like)家族蛋白的相互作用,发...F-box蛋白作为SCF(Skp1,Cullin and an F-box protein)复合体的成员,参与调节植物的生长发育过程。At5g22700为功能未知的F-box基因家族成员。本研究通过酵母双杂交分析At5g22700蛋白与ASK(Arabidopsis-SKP1-like)家族蛋白的相互作用,发现At5g22700蛋白的F-box结构域与ASK4蛋白相互作用。实时定量PCR分析该基因在不同组织器官中的表达,发现该基因在根和花中的表达量最高,说明At5g22700可能在根和花的发育中具有重要作用。以At5g22700基因的T-DNA插入突变体和过量表达转基因株系为材料,分析不同光照条件下幼苗的表型,发现蓝光下At5g22700过量表达转基因幼苗的主根比野生型长。这些研究结果表明,At5g22700在植物体内可能形成SCF复合体,并在植物幼苗主根伸长生长中起促进作用。展开更多
[Objectives] To increase the survival rate of tissue culture seedlings of Dendrobium officinale,and optimize the conditions of rooting medium by the response surface methodology( RSM). [Methods]The effects of 6-BA con...[Objectives] To increase the survival rate of tissue culture seedlings of Dendrobium officinale,and optimize the conditions of rooting medium by the response surface methodology( RSM). [Methods]The effects of 6-BA concentration,NAA concentration,potato amount and the amount of mashed banana on the growth of seedlings were determined by single factor experiment and were analyzed by Box-Behnken design and response surface methodology. [Results]The optimal culture conditions: rooting medium 1/2 MS + 6-BA 0. 24 mg/L + mashed banana 87. 63 g/L + potato 89. 30 g/L + NAA 0. 52 mg/L + sucrose 20. 0 g/L + activated carbon 4. 0 g/L + agar 7. 0 g/L,p H 5. 8,and light intensity 2 000 Lx. [Conclusions]The model established by response surface methodology has a good predictability and could be used to optimize the conditions of tissue culture and rooting medium of D. officinale.展开更多
Little is known regarding the ability of protozoans to transfer phosphates and improve maize growth.The objective of this study was to determine whether Colpoda cucullus could improve the maize phosphorus(P) level by ...Little is known regarding the ability of protozoans to transfer phosphates and improve maize growth.The objective of this study was to determine whether Colpoda cucullus could improve the maize phosphorus(P) level by transferring phosphate.In this three-compartment root box study,the soil in the outer compartment was inoculated with the common ciliate,C.cucullus,together with the addition of either KH_(2)^(32)PO_(4),rock phosphate(RP),super phosphate(SP) or ammonium phosphate(AP),and then maize was grown in the inner compartment.The results showed that the maize plants grown in the soil inoculated with C.cucullus had much higher ^(32)P radioactivity than the control.Colpoda cucullus inoculation resulted in significant increases in dry matter by up to 25.07%,and nitrogen(N),P and potassium(K) absorption by 1–36%(P<0.05).Soil available P in the inner compartment of the root box was also enhanced by at least 30% due to the ciliate inoculation(P<0.05).It was therefore suggested that phosphates might be transported from the outer to inner compartments by the inoculated C.cucullus and then absorbed by the maize plant.展开更多
文摘To study the influence of photoperiod on roots differentiation in the Tunisian grapevine (Vitis vinifera L.) cultivar Perle noir, roots and callus initiation were analyzed under three different conditions of day length: long day (LD), short day (SD) and darkness (D). The photoperiod influenced the number of callus and roots per cuttings;it has a significant effect on the roots and callus initiation. Expression profile analysis of six MADS-box genes (VTM8, VSEP2, VAG12, VAG17-1, VAG17-2 and VSOC1.3) during root and callus development is in agreement with the above-mentioned observation. The expression of the MADS-box genes during root and callus development fluctuated in a tissue-dependent manner. These data suggest that all genes are expressed in roots under three photoperiods. Total darkness gives the number of the most important root per cutting compared to the other two conditions. This photoperiodic condition gave the most important expression of the studied genes VAG12, VAG17-2, VAG17-1, VTM8 and VSEP2 transcripts were not found in callus grown in the dark or in LD conditions, respectively. VSOC1.3 transcripts were not found in callus grown in the dark or in SD conditions, respectively. Transcript abundance of VTM8 and VSOC1 was highest in LD.
文摘F-box蛋白作为SCF(Skp1,Cullin and an F-box protein)复合体的成员,参与调节植物的生长发育过程。At5g22700为功能未知的F-box基因家族成员。本研究通过酵母双杂交分析At5g22700蛋白与ASK(Arabidopsis-SKP1-like)家族蛋白的相互作用,发现At5g22700蛋白的F-box结构域与ASK4蛋白相互作用。实时定量PCR分析该基因在不同组织器官中的表达,发现该基因在根和花中的表达量最高,说明At5g22700可能在根和花的发育中具有重要作用。以At5g22700基因的T-DNA插入突变体和过量表达转基因株系为材料,分析不同光照条件下幼苗的表型,发现蓝光下At5g22700过量表达转基因幼苗的主根比野生型长。这些研究结果表明,At5g22700在植物体内可能形成SCF复合体,并在植物幼苗主根伸长生长中起促进作用。
基金Supported by Scientific and Technological Planning Project of Pu'er City of Yunnan Province(2012kj007)
文摘[Objectives] To increase the survival rate of tissue culture seedlings of Dendrobium officinale,and optimize the conditions of rooting medium by the response surface methodology( RSM). [Methods]The effects of 6-BA concentration,NAA concentration,potato amount and the amount of mashed banana on the growth of seedlings were determined by single factor experiment and were analyzed by Box-Behnken design and response surface methodology. [Results]The optimal culture conditions: rooting medium 1/2 MS + 6-BA 0. 24 mg/L + mashed banana 87. 63 g/L + potato 89. 30 g/L + NAA 0. 52 mg/L + sucrose 20. 0 g/L + activated carbon 4. 0 g/L + agar 7. 0 g/L,p H 5. 8,and light intensity 2 000 Lx. [Conclusions]The model established by response surface methodology has a good predictability and could be used to optimize the conditions of tissue culture and rooting medium of D. officinale.
基金funded by the National Natural Science Foundation of China (41877059)。
文摘Little is known regarding the ability of protozoans to transfer phosphates and improve maize growth.The objective of this study was to determine whether Colpoda cucullus could improve the maize phosphorus(P) level by transferring phosphate.In this three-compartment root box study,the soil in the outer compartment was inoculated with the common ciliate,C.cucullus,together with the addition of either KH_(2)^(32)PO_(4),rock phosphate(RP),super phosphate(SP) or ammonium phosphate(AP),and then maize was grown in the inner compartment.The results showed that the maize plants grown in the soil inoculated with C.cucullus had much higher ^(32)P radioactivity than the control.Colpoda cucullus inoculation resulted in significant increases in dry matter by up to 25.07%,and nitrogen(N),P and potassium(K) absorption by 1–36%(P<0.05).Soil available P in the inner compartment of the root box was also enhanced by at least 30% due to the ciliate inoculation(P<0.05).It was therefore suggested that phosphates might be transported from the outer to inner compartments by the inoculated C.cucullus and then absorbed by the maize plant.
