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血根碱调节RhoA/ROCK信号通路对糖尿病性骨质疏松症大鼠骨代谢和炎性损伤的影响
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作者 周飞 易松 +1 位作者 岳建彪 张上上 《免疫学杂志》 2026年第2期81-86,共6页
目的探究血根碱调节Ras同源基因家族成员A(RhoA)/Rho相关激酶(ROCK)信号通路对糖尿病性骨质疏松症大鼠骨代谢和炎性损伤的影响。方法取60只健康雄性8周龄SD大鼠,分为正常组(未予任何处理)、模型组(腹腔注射60 mg/kg 0.2%链脲佐菌素+骨... 目的探究血根碱调节Ras同源基因家族成员A(RhoA)/Rho相关激酶(ROCK)信号通路对糖尿病性骨质疏松症大鼠骨代谢和炎性损伤的影响。方法取60只健康雄性8周龄SD大鼠,分为正常组(未予任何处理)、模型组(腹腔注射60 mg/kg 0.2%链脲佐菌素+骨密度降低)、低剂量血根碱组(L-血根碱组)、高剂量血根碱组(H-血根碱组)、H-血根碱+抑制剂组,每组12只,L-血根碱组、H-血根碱组和H-血根碱+抑制剂组分别为造模成功大鼠予2.50 mg/(kg·d)血根碱灌胃+等量0.9%氯化钠注射液腹腔注射、6.25 mg/(kg·d)血根碱灌胃+等量0.9%氯化钠注射液腹腔注射、6.25 mg/(kg·d)血根碱灌胃+40 mg/(kg·d)Rhosin腹腔注射。各组大鼠处理8周后,使用双能X线吸收测定扫描系统测量骨密度;检测血清钙、骨钙素(OC)、骨保护素(OPG)、碱性磷酸酶(ALP)水平;行生物力学测试检测各组大鼠股骨最大载荷;HE染色观察各组大鼠胰腺组织病理学变化与股骨组织形态学;酶联免疫吸附试验检测各组大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)水平;Western blot实验检测各组大鼠股骨组织RhoA、ROCK1和ROCK2蛋白表达。结果与正常组相比,模型组大鼠骨密度和股骨最大载荷降低,胰岛和股骨组织被破坏,血清钙、OC、OPG含量及股骨组织RhoA、ROCK1、ROCK2蛋白表达降低,血清ALP、IL-1β、TNF-α、IL-6水平升高(P<0.05);与模型组相比,L-血根碱组、H-血根碱组大鼠骨密度和股骨最大载荷升高,胰岛和股骨组织损伤减轻,血清钙、OC、OPG含量及股骨组织RhoA、ROCK1、ROCK2蛋白表达升高,血清ALP、IL-1β、TNF-α、IL-6水平降低,且H-血根碱组上述指标变化更显著(P<0.05);与H-血根碱组相比,H-血根碱+抑制剂组逆转了血根碱对大鼠骨代谢和炎性损伤的影响。结论血根碱通过激活RhoA/ROCK信号通路改善糖尿病性骨质疏松症大鼠骨代谢,并减轻了其炎性损伤。 展开更多
关键词 骨质疏松症 糖尿病性 血根碱 大鼠 RhoA/rock信号通路 骨代谢 炎性损伤
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RhoA/ROCK信号通路在椎间盘退变中的作用
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作者 杜健强 郭子煜 +3 位作者 姚晨阳 冯其金 古恩鹏 王琳珏 《生理科学进展》 北大核心 2026年第1期52-59,共8页
椎间盘由髓核、纤维环和软骨终板组成,对维持脊柱正常生理功能至关重要。椎间盘退变(intervertebral disc degeneration,IDD)是导致腰背痛等脊柱退行性疾病的主要病理基础,给人们的健康状况造成极大的困扰。然而目前对IDD的分子机制仍... 椎间盘由髓核、纤维环和软骨终板组成,对维持脊柱正常生理功能至关重要。椎间盘退变(intervertebral disc degeneration,IDD)是导致腰背痛等脊柱退行性疾病的主要病理基础,给人们的健康状况造成极大的困扰。然而目前对IDD的分子机制仍然缺乏清晰的了解,导致缺乏有效的靶向干预措施。RAS同源家族成员A(RAS homolog family member A,RhoA)/Rho相关蛋白激酶(Rho-associated protein kinase,ROCK)信号通路是调节细胞收缩、迁移和生长的经典通路。其被激活后可参与调控细胞骨架重塑、细胞外基质代谢、生物钟节律、细胞表型改变、细胞衰老及死亡等环节,进而影响IDD的病理进程。深入探究RhoA/ROCK信号通路在IDD中的作用,不仅能揭示疾病发生的分子生物学机制,也有望为研发靶向该通路的治疗策略提供理论依据。 展开更多
关键词 椎间盘退变 RhoA/rock 信号传导 作用机制
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温阳利水方通过激活RhoA/ROCK通路改善慢性肺源性心脏病小鼠心肌细胞焦亡的机制
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作者 陈昕芳 李红 +2 位作者 苏士成 吴峰妹 黄辉 《辽宁中医杂志》 北大核心 2026年第2期204-208,F0003,共6页
目的探讨温阳利水方通过激活RhoA/ROCK通路改善慢性肺源性心脏病小鼠心肌细胞焦亡的机制。方法60只健康雄性C57BL/6小鼠用于实验研究。将小鼠暴露于烟雾和高压氧舱中诱导慢性肺源性心脏病(chronic pulmonary heart disease,CPHD)模型。... 目的探讨温阳利水方通过激活RhoA/ROCK通路改善慢性肺源性心脏病小鼠心肌细胞焦亡的机制。方法60只健康雄性C57BL/6小鼠用于实验研究。将小鼠暴露于烟雾和高压氧舱中诱导慢性肺源性心脏病(chronic pulmonary heart disease,CPHD)模型。将小鼠分为以下3组:对照组(正常条件下饲养的小鼠,接受清洁空气和0.9%氯化钠溶液作为模型组和温阳利水方组的对照,n=20),模型组(如前所述建立的CPF模型,n=20),温阳利水方组(在模型小鼠的基础上给予温阳利水方药剂治疗,n=20)。通过qPCR分析RhoA、ROCK1和ROCK2 mRNA表达。通过蛋白质印迹分析小鼠心肌组织p-MBS/t-MBS比值。通过酶联免疫吸附测定(ELISA)检测小鼠外周血炎症因子肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、白细胞介素1β(interleukin-1β,IL-1β)和白细胞介素6(interleukin-6,IL-6)水平。组织学分析小鼠心肌受损情况。通过超声心动图检测小鼠心肌功能。通过PI染色检测焦亡细胞。通过蛋白质印迹分析焦亡相关因子高迁移率族蛋白(HMGB1)和热休克蛋白70(HSP70)的表达。结果模型组RhoA、ROCK1和ROCK2 mRNA表达较对照组升高(P<0.05),温阳利水方组RhoA、ROCK1和ROCK2 mRNA表达较模型组降低(P<0.05)。模型组p-MBS/t-MBS比值较对照组升高(P<0.05),温阳利水方组p-MBS/t-MBS比值较模型组降低(P<0.05)。模型组TNF-α、IL-1β和IL-6水平较对照组升高(P<0.05),温阳利水方组TNF-α、IL-1β和IL-6水平较模型组降低(P<0.05)。HE染色显示,与对照组小鼠心肌相比,模型组小鼠心肌细胞排列紊乱,细胞肥大,炎症细胞浸润明显(P<0.05),与模型组相比,温阳利水方显著改善了CPHD引起的病理改变(P<0.05)。马松染色作为纤维化的病理检查表明,CPHD可导致小鼠严重的心肌纤维化,温阳利水方治疗可显著减少胶原沉积(P<0.05)。模型组小鼠左室收缩压(left ventricular systolic pressure,LVSP)、左室射血分数(left ventricular ejection fraction,LVEF)和心搏量(stroke volume,SV)较对照组降低(P<0.05),温阳利水方组LVSP、LVEF和SV较模型组升高(P<0.05)。模型组小鼠心肌细胞焦亡率较对照组升高(P<0.05),温阳利水方组小鼠心肌细胞焦亡率较模型组降低(P<0.05)。模型组HMGB1和HSP70的表达较对照组升高(P<0.05),温阳利水方组HMGB1和HSP70的蛋白表达较模型组降低(P<0.05)。结论温阳利水方通过激活RhoA/ROCK通路,显著改善了CPHD小鼠模型中心肌细胞焦亡和心肌结构及功能。 展开更多
关键词 温阳利水方 RhoA/rock CPHD 心肌细胞焦亡
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基于RhoA/ROCKⅡ通路探讨水蛭改善脑缺血/再灌注小鼠脑损伤的机制
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作者 赵磊 曹玉爽 +4 位作者 郭莉琛 柴丽娟 王少峡 袁庆 胡利民 《中国药理学通报》 北大核心 2026年第1期100-106,共7页
目的探究水蛭提取物(leech)调控Rho激酶(Rhoassociated coiled-coil-forming kinase,ROCK)通路保护大脑中动脉闭塞/再灌注(middle cerebral artery occlusion/reperfu-sion,MCAO/R)小鼠血脑屏障及其作用机制。方法采用大脑中动脉线栓法... 目的探究水蛭提取物(leech)调控Rho激酶(Rhoassociated coiled-coil-forming kinase,ROCK)通路保护大脑中动脉闭塞/再灌注(middle cerebral artery occlusion/reperfu-sion,MCAO/R)小鼠血脑屏障及其作用机制。方法采用大脑中动脉线栓法建立MCAO/R模型后,将SPF级C57BL/6(C57BL/6JNifdc)雄性小鼠,分为假手术(Sham)组、MCAO/R组、阿司匹林组(aspirin,13 mg·kg^(-1))、水蛭提取液组(leech,0.8、1.6、2.4 mL·kg^(-1))共6组。TTC染色观察脑梗死体积,mNSS评分评价神经缺损情况;HE染色及Nissl染色观察各组小鼠脑缺血半暗带病理改变;免疫荧光检测Laminin,PDGFRβ/Biotinylated LEL免疫荧光双染检测脑缺血半暗带周细胞与脑微血管内皮细胞解离情况;Western blot检测RhoA、ROCKⅡ蛋白,紧密连接相关蛋白ZO-1、Occludin、Clau-din-5,基底膜(BM)相关蛋白层粘连蛋白(LN)、纤维连接蛋白(FN)及金属基质蛋白酶MMP-2、MMP-9蛋白,以及p-MLC/MLC蛋白表达情况。结果Leech明显改善小鼠神经缺损,减轻脑梗死体积(P<0.05,P<0.01);Western blot结果显示,leech可上调ZO-1、Occludin、Claudin-5、Laminin、Fibronectin蛋白表达,降低p-MLC/MLC、RhoA、ROCKⅡ、MMP-2、MMP-9蛋白表达(P<0.05,P<0.01);免疫荧光结果显示,leech可明显提高Laminin及PDGFRβ/Biotinylated LEL免疫荧光双染的表达。结论Leech可以改善MCAO/R小鼠脑缺血半暗带BBB损伤,其机制可能是通过调节RhoA/ROCKⅡ信号通路有关。 展开更多
关键词 水蛭 脑缺血/再灌注损伤 RhoA/rockⅡ信号通路 血脑屏障 紧密连接 基质金属蛋白酶
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FOXK1通过上调ROCK1表达促进食管鳞状细胞癌的恶性生物学行为
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作者 武俊红 杨霞 +4 位作者 许环琛 王歆皓 胡照坤 路军涛 郭炜 《临床与实验病理学杂志》 北大核心 2026年第1期29-37,共9页
目的探讨FOXK1在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)中的表达、功能及其潜在的分子调控机制。方法常规培养ESCC细胞系KYSE-150、KYSE-170和TE-1;并使用转染试剂将核酸和质粒转染至各组细胞。结合数据库分析和qRT-... 目的探讨FOXK1在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)中的表达、功能及其潜在的分子调控机制。方法常规培养ESCC细胞系KYSE-150、KYSE-170和TE-1;并使用转染试剂将核酸和质粒转染至各组细胞。结合数据库分析和qRT-PCR检测FOXK1在ESCC组织和细胞中的表达;开展生物信息学分析预测下游靶基因,并以ROCK1为候选基因,采用qRT-PCR检测其表达,双荧光素酶报告实验验证FOXK1对ROCK1启动子的转录调控作用。通过平板克隆实验、划痕愈合实验及Transwell小室实验检测各组细胞的克隆形成、迁移及侵袭能力。应用qRT-PCR和Western blot法检测各组细胞中上皮-间质转化(epithelialmesenchymal transition,EMT)关键分子的表达;免疫荧光实验检测F-actin的荧光强度。结果FOXK1在ESCC组织和细胞中呈高表达,其中KYSE-150、KYSE-170和TE-1细胞的表达量分别约为正常食管上皮细胞的40.0倍、17.9倍和24.8倍(P<0.05)。ROCK1为FOXK1的潜在下游靶基因,两者表达水平呈正相关(P<0.05),且ROCK1在ESCC细胞系中亦高表达。过表达及敲低FOXK1会相应上调或下调ROCK1的表达,并证实FOXK1可结合ROCK1启动子发挥转录调控作用。功能方面,过表达FOXK1促进ESCC细胞增殖、迁移和侵袭,敲低ROCK1抑制上述表型,两者同时作用可部分逆转由FOXK1过表达所引起的促肿瘤效应。机制层面,敲低ROCK1使EMT进程关键分子CDH2、vimentin和ZEB1的mRNA及蛋白水平均下降;过表达FOXK1增强F-actin的聚合,敲低ROCK1削弱F-actin的聚合,两者同时作用可部分逆转FOXK1对F-actin的促聚合效应。结论FOXK1通过上调ROCK1的表达促进ESCC细胞的迁移和侵袭,提示FOXK1可能作为ESCC的潜在诊断和治疗靶点。 展开更多
关键词 食管肿瘤 鳞状细胞癌 FOXK1 rock1 上皮-间质转化 细胞骨架
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基于RhoA/ROCK1通路探讨花旗松素对缺氧/复氧诱导的心肌细胞损伤的影响
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作者 徐颖 李丽华 +1 位作者 刘晨 张宁 《河北医学》 2026年第2期240-247,共8页
目的:基于RhoA/ROCK1通路,探讨花旗松素(TAX)对缺氧/复氧(H/R)诱导的心肌细胞损伤的影响。方法:CCK-8筛选TAX处理H/R诱导H9C2的最佳浓度;H9C2细胞分为正常组、H/R组、H/R+TAX组、H/R+ROCK抑制剂(Y-27632)组、H/R+TAX+RhoA激活剂溶血磷脂... 目的:基于RhoA/ROCK1通路,探讨花旗松素(TAX)对缺氧/复氧(H/R)诱导的心肌细胞损伤的影响。方法:CCK-8筛选TAX处理H/R诱导H9C2的最佳浓度;H9C2细胞分为正常组、H/R组、H/R+TAX组、H/R+ROCK抑制剂(Y-27632)组、H/R+TAX+RhoA激活剂溶血磷脂酸(LPA)组。台盼蓝染色检测各组H9C2细胞存活率;试剂盒检测各组H9C2细胞LDH释放率;流式细胞仪和TUENL染色检测各组H9C2细胞凋亡率;DCFH-DA法和ELISA检测各组H9C2细胞氧化应激和炎症反应;Western blot检测各组H9C2细胞RhoA/ROCK1通路相关蛋白表达。结果:与对照组比较,H/R组H9C2细胞存活率明显降低(P<0.05);与H/R组比较,H/R+TAX 20μmoL/L组和H/R+TAX 40μmoL/L组H9C2细胞存活率明显升高(P<0.05);与H/R+TAX 40μmoL/L组比较,H/R+TAX 80μmoL/L组H9C2细胞存活率明显降低(P<0.05)。与对照组比较,H/R组H9C2细胞存活率、GSH-Px下调,LDH释放率、凋亡率以及TUENL阳性细胞数、ROS荧光强度、MDA、NOX4、IL-6、TNF-α、NLRP3、ICAM-1、RhoA、ROCK1、p-MLC2/MLC2上调(P<0.05);与H/R组比较,H/R+TAX组和H/R+Y-27632组H9C2细胞存活率、GSH-Px上调,LDH释放率、凋亡率以及TUENL阳性细胞数、ROS荧光强度、MDA、NOX4、IL-6、TNF-α、NLRP3、ICAM-1、ROCK1、p-MLC2/MLC2下调(P<0.05);与H/R+TAX组比较,H/R+TAX+LPA组H9C2细胞存活率、GSH-Px下调,LDH释放率、凋亡率以及TUENL阳性细胞数、ROS荧光强度、MDA、NOX4、IL-6、TNF-α、NLRP3、ICAM-1、RhoA、ROCK1、p-MLC2/MLC2上调(P<0.05)。结论:TAX通过抑制RhoA/ROCK1通路调控氧化应激、炎症反应和细胞凋亡,缓解H/R诱导的心肌细胞损伤。 展开更多
关键词 花旗松素 缺氧/复氧 心肌细胞 RhoA/rock1通路
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Stress-path dependency of rock shear strength influencedby shear surface integrity:Experimental and numerical investigations
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作者 Tao Li Qiang Zhang +3 位作者 Guilei Han Hongying Wang Linpo Shi Sizhe Ye 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第2期1073-1089,共17页
Excavation causes stress redistribution and affects the stress path during the shearing process of rock.The shear strength of rock varies under different stress paths,and the presence of defects reduces the shear stre... Excavation causes stress redistribution and affects the stress path during the shearing process of rock.The shear strength of rock varies under different stress paths,and the presence of defects reduces the shear strength.To further investigate this phenomenon,this study investigates the shear behaviour of rocks with different shear surface integrities under the influenceof different stress paths through laboratory tests and numerical simulations.The results indicate that the shear strength depends on the stress path and a decrease in the shear surface integrity reduces the degree of dependence.The cohesion and friction angle of the Mohr‒Coulomb criterion decrease with weakening of the shear surface integrity.For different stress paths,the direct shear strength is always greater than that of other shear stress paths.The pattern of changes in the acoustic emission count and cumulative count indirectly reflectsthe above findings.Numerical simulations further indicate that the different principal stress states and normal suppression effects during the shearing process lead to changes in the factors of crack propagation,resulting in different mechanical behaviours under various stress paths.For rocks with different integrity levels,the main reason for the different path dependences of shear strength is that the size of the area affected by shear is different.Shear failure will concentrate on the shear plane when the normal inhibition effect is greater.This study explores the mechanism of rock shear behaviour,providing a theoretical basis for establishing more accurate constitutive models and strength criteria. 展开更多
关键词 rock integrity Stress path Shear strength Crack propagation Principal stress state
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Acoustic emission characteristics of rock salt under multistage creep-fatigue loading at various temperatures
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作者 Hang Li Chunhe Yang +5 位作者 Hongling Ma Jiangyu Fang Rui Liang Wentao Li Kai Zhao J.J.K.Daemen 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第1期364-379,共16页
With the growing global demand for energy,deep underground salt caverns are emerging as a potential solution for large-scale energy storage.In this study,multistage cyclic loading tests were conducted on rock salt at ... With the growing global demand for energy,deep underground salt caverns are emerging as a potential solution for large-scale energy storage.In this study,multistage cyclic loading tests were conducted on rock salt at different temperatures in combination with real-time acoustic emission(AE)monitoring.The results show that the cumulative AE count increases stepwise with increasing cyclic stress.The peak frequency is concentrated primarily in the medium-frequency range,exhibiting a band distribution across low-,medium-,and high-frequency ranges.As the temperature increases,the proportion of low-frequency signals decreases from 14.32%to 5.76%,whereas the proportion of medium-frequency signals increases from 85.48%to 94.1%.The proportion of high-frequency signals remains relatively constant between 0.1%and 0.2%.The amplitude-count relationship of the AE signals demonstrates a strong negative power-law correlation.Furthermore,with increasing temperature,the negative power-law exponent of the amplitude gradually decreases,with the b value decreasing from 1.096 to 0.837 and the a value decreasing from 7.4871 to 6.6982.Under all four temperature conditions,the dominant failure mode in rock salt is tensile cracking.However,as the temperature increases,the proportion of tensile cracks decreases from 88.59%to 75.12%,whereas the proportion of shear cracks at 80℃is nearly double that at 20℃.This finding indicates that as the temperature increases,the ductility of the material increases,and the crack propagation mode shifts from tensile to shear.This research provides valuable insights for the design and stability assessment of salt cavern reservoirs for deep underground energy storage systems. 展开更多
关键词 rock salt Cyclic loading Temperature Acoustic emission(AE) Crack type
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Petrogenesis and tectonic significance of the Paleoproterozoic Chibaisong meta-mafic rocks in the northeastern Jiao-Liao-Ji Belt
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作者 ZHANG Jirui YU Hongchao +2 位作者 LIU Jin ZHANG Hongxiang ZHANG Yuanzhuhui 《Global Geology》 2026年第1期68-85,共18页
The Jiao-Liao-Ji Belt within the North China Craton had undergone a complex tectonic evolution,marked by extensive Paleoproterozoic magmatism that produced a diverse range of mafic to felsic magmatic rocks.These event... The Jiao-Liao-Ji Belt within the North China Craton had undergone a complex tectonic evolution,marked by extensive Paleoproterozoic magmatism that produced a diverse range of mafic to felsic magmatic rocks.These events provide valuable geological records for understanding the tectonic evolution of the Jiao-Liao-Ji Belt.This study focuses on the Paleoproterozoic Chibaisong meta-gabbro in southern Jilin,investigating the source of mafic magma,petrogenesis,and tectonic setting through systematic petrological analysis,zircon U-Pb dating,and geochemical studies.The findings contribute to constraining the dynamic mechanisms of Paleoproterozoic extensional rifting in the Jiao-Liao-Ji orogenic belt.Geochemical data indicate that the samples exhibit low SiO₂(47.99–50.66 wt.%),TiO₂(0.75–3.20 wt.%),Nb(3.22–8.09 ppm),and Ta(0.22–0.51 ppm)contents,along with high TFeO₃(11.97–15.82 wt.%)and MgO(5.67–12.66 wt.%)concentrations.They also show low Nb/La ratios and high(Th/Nb)N values,consistent with the geochemical characteristics of tholeiitic basalts.The samples display low total rare earth element concentrations,weak or absent Eu anomalies,slight enrichment in light rare earth elements,relatively flat heavy rare earth element patterns,and depletion in Nb,Ta,and Ti.The meta-gabbro from southern Jilin exhibits geochemical similarities to Paleoproterozoic metamafic rocks from other regions of the Jiao-Liao-Ji Belt,resembling enriched mid-ocean ridge basalts(E-MORB)with Nb,Ta,and Ti depletion.Integration of previous studies and geochemical data suggests that the 2.16–2.10 Ga metamafic rocks in the Jiao-Liao-Ji Belt experienced contamination by older crustal material.These results indicate that the 2.16–2.10 Ga metamafic rocks are unlikely to be associated with island arc,continental arc,or mid-ocean ridge settings.Instead,they are more likely related to intraplate magmatism associated with continental rifting. 展开更多
关键词 Jiao-Liao-Ji Belt North China Craton PALEOPROTEROZOIC southern Jilin region metamafic rocks
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Improved expert system of rockburst intensity level prediction based on machine learning and data-driven:Supported by 1114 rockburst cases in 197 rock underground projects
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作者 PANG Hong-li GONG Feng-qiang +1 位作者 GAO Ming-zhong DAI Jin-hao 《Journal of Central South University》 2026年第1期335-356,共22页
Accurate prediction of rockburst intensity levels is crucial for ensuring the safety of deep hard rock engineering construction.This paper introduced an expert system for rockburst intensity level prediction that empl... Accurate prediction of rockburst intensity levels is crucial for ensuring the safety of deep hard rock engineering construction.This paper introduced an expert system for rockburst intensity level prediction that employs machine learning algorithms as the basis for its inference rules.The system comprises four modules:a database,a repository,an inference engine,and an interpreter.A database containing 1114 rockburst cases was used to construct 357 datasets that serve as the repository for the expert system.Additionally,19 types of machine learning algorithms were used to establish 6783 micro-models to construct cognitive rules within the inference engine.By integrating probability theory and marginal analysis,a fuzzy scoring method based on the SoftMax function was developed and applied to the interpreter for rockburst intensity level prediction,effectively restoring the continuity of rockburst characteristics.The research results indicate that ensemble algorithms based on decision trees are more effective in capturing the characteristics of rockburst.Key factors for accurate prediction of rockburst intensity include uniaxial compressive strength,elastic energy index,the maximum principal stress,tangential stress,and their composite indicators.The accuracy of the proposed rockburst intensity level prediction expert system was verified using 20 engineering rockburst cases,with predictions aligning closely with the actual rockburst intensity levels. 展开更多
关键词 rock mechanics rockBURST rockburst intensity level prediction expert system machine learning supervised learning
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Rock strength assessment in tectonically deformed calcareous rocks integrating equotip,ultrasound velocity,and geo-structural fracture analysis
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作者 Elisa Mammoliti Danica Jablonská +3 位作者 Antonio Ferretti Michele Morici Stefano Mazzoli Mirko Francioni 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第1期196-213,共18页
In complex geological environments,the analysis of drill cores to determine rock strength can be challenging due to the wide variability in the degree of fracturing,leading to subjectivity in the collection of represe... In complex geological environments,the analysis of drill cores to determine rock strength can be challenging due to the wide variability in the degree of fracturing,leading to subjectivity in the collection of representative samples for uniaxial compressive strength testing.This study evaluates non-destructive techniques on calcareous rocks with different tectonic deformations,including Equotip hardness,ultrasound P-wave velocity,thin section analysis,and calcimetry,integrated with photogrammetric fracture analysis.The investigated carbonate rock samples are sourced from drill cores derived from the Umbria-Marche fold and thrust belt(northern Apennines,Italy),including a gently dipping limb of an anticline,a hinge zone of an anticline,and a fault zone associated with a thrust.Fracture intensity,quantified by the P21 parameter using photogrammetric techniques on pre-loading rock samples,is assessed alongside macroscopic identification of discontinuities(such as stylolites,veins,and joints)using marker colours to monitor failures during uniaxial compression testing.Empirical correlations depicted by single and multi-linear relationships indicate a strong dependence between the mechanical and physical properties of limestones.Both Equotip and P-wave velocity are influenced by fracture intensity,but P-wave velocity varies significantly with discontinuity orientation,especially at 45°-90°.To refine uniaxial compressive strength predictions and mitigate multicollinearity,statistical approaches,including linear and multilinear regression,Principal Component Analysis and Gaussian Process Regression,were tested.Findings improve the reliability of non-destructive techniques for assessing rock strength in structurally complex settings,with implications for geotechnical applications. 展开更多
关键词 Non-destructive testing Uniaxial compressive strength Equotip Ultrasound pulse velocity FRACTURING Calcareous rocks
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Fracture characteristics and fracture interface buckling mechanism of cantilever rock mass under non-uniformly distributed load
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作者 Wenlong Shen Ziqiang Chen +7 位作者 Meng Wang Jianbiao Bai Zhengyuan Qin Tongqiang Xiao Ningkang Meng Juntao Liu Yan Gai Hua Nan 《International Journal of Mining Science and Technology》 2026年第2期375-397,共23页
This study examined non-uniform loading in goaf cantilever rock masses via testing,modeling,and mechanical analysis to solve instantaneous fracture and section buckling from mining abutment pressure.The study investig... This study examined non-uniform loading in goaf cantilever rock masses via testing,modeling,and mechanical analysis to solve instantaneous fracture and section buckling from mining abutment pressure.The study investigates the non-uniform load gradient effect on fracture characteristics,including load characteristics,fracture location,fracture distribution,and section roughness.A digital model for fracture interface buckling analysis was developed,elucidating the influence of non-uniform load gradients on Fracture Interface Curvature(FIC),Buckling Rate of Change(BRC),and Buckling Domain Field(BDF).The findings reveal that nonlinear tensile stress concentration and abrupt tensile-compressive-shear strain mutations under non-uniform loading are fundamental mechanisms driving fracture path buckling in cantilever rock mass structures.The buckling process of rock mass under non-uniform load can be divided into two stages:low load gradient and high gradient load.In the stage of low gradient load,the buckling behavior is mainly reflected in the compression-shear fracture of the edge.In the stage of high gradient load,a buckling band along the loading direction is gradually formed in the rock mass.These buckling principles establish a theoretical basis for accurately characterizing bearing fractures,fracture interface instability,and vibration sources within overlying cantilever rock masses in goaf. 展开更多
关键词 Cantilever rock mass Non-uniformly distributed load Fracture characteristics Buckling fracture Digital model
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Process analysis of columnar dangerous rock mass collapse in the Three Gorges Reservoir Area based on 3D MPM
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作者 CHENG Shulou HUANG Bolin +3 位作者 ZHANG Peng LI Qiuwang DONG Xingchen Qin Panpan 《Journal of Mountain Science》 2026年第1期237-253,共17页
The Three Gorges Reservoir area hosts numerous high-risk columnar rock masses,but predicting their full-process of collapse dynamics in narrow-deep terrains remains challenging.This study investigated the Changdongzi ... The Three Gorges Reservoir area hosts numerous high-risk columnar rock masses,but predicting their full-process of collapse dynamics in narrow-deep terrains remains challenging.This study investigated the Changdongzi columnar dangerous rock mass(CCDRM)through field surveys and simulated its collapse dynamics with Material Point Method(MPM),and analyzed the collapse-diffusionsurge generation mechanisms.Field investigations revealed a 0.8 m-thick lotus-like extrusion structure at the base,severely degraded by cyclic water fluctuations,predisposing the rock mass to base-crushing failure.Numerical simulations indicate collapse velocities up to 42 m/s,amplified spreading with a 318 m deposition range and 11 m maximum thickness,and two major collisions causing 50%-60%velocity reduction.Gully topography buffers kinetic energy,retaining 5%debris on slopes and channeling 7%into the river.High-speed river impact generates a 20 m-diameter cavity,producing an 11 m surge,identifying the Guandukou Town waterfront 1.2 km downstream as a high-risk zone.These results clarify the collapse mechanisms of columnar dangerous rock mass and inform its hazard mitigation strategies. 展开更多
关键词 Dangerous rock mass Material Point Method Three-dimensional numerical simulation Disaster chain SURGE
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Energy relief effect of real-time drilling to prevent rockburst in high-stress rock
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作者 Zhichao He Fengqiang Gong +2 位作者 Li Ren Weimin Yang Xuezhen Wu 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第2期1460-1475,共16页
To investigate the energy relief effect of real-time drilling in preventing rockburst in high-stress rock,a series of high-stress real-time drilling uniaxial compression tests were conducted on red sandstone specimens... To investigate the energy relief effect of real-time drilling in preventing rockburst in high-stress rock,a series of high-stress real-time drilling uniaxial compression tests were conducted on red sandstone specimens using the SG4500 drilling rig.Results showed that the mechanical behavior(i.e.peak strength and rockburst intensity)of the rock was weakened under high-stress real-time drilling and exhibited a downward trend as the drilling diameter increased.The real-time drilling energy dissipation index(ERD)was proposed to characterize the energy relief during high-stress real-time drilling.The ERD exhibited a linear increase with the real-time drilling diameter.Furthermore,the elastic strain energy of post-drilling rock showed a linear relationship with the square of stress across different stress levels,which also applied to the peak elastic strain energy and the square of peak stress.This findingreveals the intrinsic link between the weakening effect of peak elastic strain energy and peak strength due to high-stress real-time drilling,confirmingthe consistency between energy relief and pressure relief effects.By establishing relationships among rockburst proneness,peak elastic strain energy,and peak strength,it was demonstrated that high-stress real-time drilling reduces rockburst proneness through energy dissipation.Specifically,both peak elastic strain energy and rockburst proneness decreased with larger drill bit diameters,consistent with reductions in peak strength,rockburst intensity,and fractal dimensions of high-stress real-time drilled rock.These results validate the energy relief mechanism of real-time drilling in mitigating rockburst risks. 展开更多
关键词 rock mechanics rockBURST Real-time drilling Drilling energy relief Energy storage capacity rockburst proneness
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Exploring the thermal evolution of highly-to over-mature source rocks using Raman spectroscopy and geochemical signatures
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作者 Fu-hua Shang Xiao-peng Sun +3 位作者 Shu-wei Ma Yu-tong Pang Guan-qun Zhou Ke Miao 《China Geology》 2026年第1期75-85,共11页
This study investigated the heterogeneous responses of organic matter(OM)in highly-to over-mature source rocks during thermal maturation.An integrated analysis was conducted on the Raman spectroscopic and geochemical ... This study investigated the heterogeneous responses of organic matter(OM)in highly-to over-mature source rocks during thermal maturation.An integrated analysis was conducted on the Raman spectroscopic and geochemical signatures of shales from the Lower Silurian Longmaxi Formation and the Lower Cambrian Qiongzhusi Formation,as well as anthracites from the Lower Permian Shanxi–Formation and the Upper Carboniferous Taiyuan Formation(collectively referred to as the Shanxi Taiyuan Formations).Additionally,burial and thermal evolution modeling was employed to support the analysis.A systematic assessment of Raman spectral parameters(e.g.,the positions and intensity ratio of the D and G bands)revealed robust correlations between the thermal history patterns of source rocks and molecular structural evolution parameters.The subsequent mechanistic quantification demonstrated that the maturation state of the source rocks was subjected to the hierarchical control of three principal factors:Peak heating temperature,the duration of sustained thermal intensity,and effective maturation duration.In addition,comparative analyses demonstrated that the anthracites attained higher structural ordering under sustained thermal conditions.This contrasts with the disordered carbon matrices observed in the intermittently heated shales.Raman spectroscopy further revealed broader variations in the D and G band intensities of the Longmaxi Formation compared to the Qiongzhusi Formation.This difference is associated with their different thermal histories.The thermal burial histories confirm that shales in the Longmaxi Formation underwent thermal exposure at lower peak temperatures over a shorter duration compared to those in the Qiongzhusi Formation.Finally,this study established a maturity calibration model for over-mature source rocks through a systematic correlation between Raman peak height ratios(R_(D/G))and vitrinite reflectance(R_(o)). 展开更多
关键词 Raman spectroscopy Overmature source rock Qiongzhusi Formation Longmaxi Formation ANTHRACITE Mineral exploration engineering
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Experimental study on real-time monitoring of surrounding rock 3D wave velocity structure and failure zone in deep tunnels
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作者 Hongyun Yang Chuandong Jiang +4 位作者 Yong Li Zhi Lin Xiang Wang Yifei Wu Wanlin Feng 《International Journal of Mining Science and Technology》 2026年第2期423-437,共15页
An innovative real-time monitoring method for surrounding rock damage based on microseismic time-lapse double-difference tomography is proposed for delayed dynamic damage identification and insufficient detection of a... An innovative real-time monitoring method for surrounding rock damage based on microseismic time-lapse double-difference tomography is proposed for delayed dynamic damage identification and insufficient detection of adverse geological conditions in deep-buried tunnel construction.The installation techniques for microseismic sensors were optimized by mounting sensors at bolt ends which significantly improves signal-to-noise ratio(SNR)and anti-interference capability compared to conventional borehole placement.Subsequently,a 3D wave velocity evolution model that incorporates construction-induced disturbances was established,enabling the first visualization of spatiotemporal variations in surrounding rock wave velocity.It finds significant wave velocity reduction near the tunnel face,with roof and floor damage zones extending 40–50 m;wave velocities approaching undisturbed levels at 15 m ahead of the working face and on the laterally undisturbed side;pronounced spatial asymmetry in wave velocity distribution—values on the left side exceed those on the right,with a clear stress concentration or transition zone located 10–15 m;and systematically lower velocities behind the face than in front,indicating asymmetric rock damage development.These results provide essential theoretical support and practical guidance for optimizing dynamic construction strategies,enabling real-time adjustment of support parameters,and establishing safety early warning systems in deep-buried tunnel engineering. 展开更多
关键词 Deep-buried tunnel Microseismic monitoring Wave velocity tomography Surrounding rock damage zone Real-time monitoring
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Multi-scale quantitative study on cemented tailings and waste-rock backfill under different loading rates
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作者 YIN Sheng-hua CHEN Jun-wei +4 位作者 YAN Ze-peng ZENG Jia-lu ZHOU Yun YANG Jian ZHANG Fu-shun 《Journal of Central South University》 2026年第1期357-374,共18页
The development of metallic mineral resources generates a significant amount of solid waste,such as tailings and waste rock.Cemented tailings and waste-rock backfill(CTWB)is an effective method for managing and dispos... The development of metallic mineral resources generates a significant amount of solid waste,such as tailings and waste rock.Cemented tailings and waste-rock backfill(CTWB)is an effective method for managing and disposing of this mining waste.This study employs a macro-meso-micro testing method to investigate the effects of the waste rock grading index(WGI)and loading rate(LR)on the uniaxial compressive strength(UCS),pore structure,and micromorphology of CTWB materials.Pore structures were analyzed using scanning electron microscopy(SEM)and mercury intrusion porosimetry(MIP).The particles(pores)and cracks analysis system(PCAS)software was used to quantitatively characterize the multi-scale micropores in the SEM images.The key findings indicate that the macroscopic results(UCS)of CTWB materials correspond to the microscopic results(pore structure and micromorphology).Changes in porosity largely depend on the conditions of waste rock grading index and loading rate.The inclusion of waste rock initially increases and then decreases the UCS,while porosity first decreases and then increases,with a critical waste rock grading index of 0.6.As the loading rate increases,UCS initially rises and then falls,while porosity gradually increases.Based on MIP and SEM results,at waste rock grading index 0.6,the most probable pore diameters,total pore area(TPA),pore number(PN),maximum pore area(MPA),and area probability distribution index(APDI)are minimized,while average pore form factor(APF)and fractal dimension of pore porosity distribution(FDPD)are maximized,indicating the most compact pore structure.At a loading rate of 12.0 mm/min,the most probable pore diameters,TPA,PN,MPA,APF,and APDI reach their maximum values,while FDPD reaches its minimum value.Finally,the mechanism of CTWB materials during compression is analyzed,based on the quantitative results of UCS and porosity.The research findings play a crucial role in ensuring the successful application of CTWB materials in deep metal mines. 展开更多
关键词 cemented backfill waste rock loading rate multi-scale analysis mercury intrusion porosimetry pore structure MICROMORPHOLOGY
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Automated recognition of rock discontinuity in underground engineering using geometric feature analysis
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作者 Adili Rusuli Xiaojun Li +1 位作者 Yuyun Wang Yi Rui 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第2期1016-1033,共18页
Discontinuities in rock masses critically impact the stability and safety of underground engineering.Mainstream discontinuities identificationmethods,which rely on normal vector estimation and clustering algorithms,su... Discontinuities in rock masses critically impact the stability and safety of underground engineering.Mainstream discontinuities identificationmethods,which rely on normal vector estimation and clustering algorithms,suffer from accuracy degradation,omission of critical discontinuities when orientation density is unevenly distributed,and need manual intervention.To overcome these limitations,this paper introduces a novel discontinuities identificationmethod based on geometric feature analysis of rock mass.By analyzing spatial distribution variability of point cloud and integrating an adaptive region growing algorithm,the method accurately detects independent discontinuities under complex geological conditions.Given that rock mass orientations typically follow a Fisher distribution,an adaptive hierarchical clustering algorithm based on statistical analysis is employed to automatically determine the optimal number of structural sets,eliminating the need for preset clusters or thresholds inherent in traditional methods.The proposed approach effectively handles diverse rock mass shapes and sizes,leveraging both local and global geometric features to minimize noise interference.Experimental validation on three real-world rock mass models,alongside comparisons with three conventional directional clustering algorithms,demonstrates superior accuracy and robustness in identifying optimal discontinuity sets.The proposed method offers a reliable and efficienttool for discontinuities detection and grouping in underground engineering,significantlyenhancing design and construction outcomes. 展开更多
关键词 Underground engineering rock mass discontinuity Orientation grouping Fisher distribution 3D point cloud Automated recognition
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Quantifying and mapping the heterogeneity of rock joint roughness and shear strength for rapid field assessment
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作者 Changshuo Wang Chen Huang +4 位作者 Rui Yong Guangjian Liu Pengju An Zhongjun Ma Jibo Qin 《International Journal of Mining Science and Technology》 2026年第1期149-167,共19页
Rock mass stability is significantly influenced by the heterogeneity of rock joint roughness and shear strength.While modern technology facilitates assessing roughness heterogeneity,evaluating shear strength heterogen... Rock mass stability is significantly influenced by the heterogeneity of rock joint roughness and shear strength.While modern technology facilitates assessing roughness heterogeneity,evaluating shear strength heterogeneity remains challenging.To address this,this study first captures the morphology of large-scale(1000 mm × 1000 mm) slate and granite joints via 3D laser scanning.Analysis of these surfaces and corresponding push/pull tests on carved specimens revealed a potential correlation between the heterogeneity of roughness and shear strength.A comparative evaluation of five statistical metrics identified information entropy(Hs) as the most robust indicator for quantifying rock joint heterogeneity.Further analysis using Hsreveals that the heterogeneity is anisotropic and,critically,that shear strength heterogeneity is governed not only by roughness heterogeneity but is also significantly influenced by the mean roughness value,normal stress,and intact rock tensile strength.Consequently,a simple comparison of roughness Hsvalues is insufficient for reliably comparing shear strength heterogeneity.To overcome this limitation,a theoretical framework is developed to explicitly map fundamental roughness statistics(mean and heterogeneity) to shear strength heterogeneity.This framework culminates in a practical workflow that allows for the rapid,field-based assessment of shear strength heterogeneity using readily obtainable rock joint roughness data. 展开更多
关键词 rock joint heterogeneity ROUGHNESS Shear strength Information entropy Push/pull test Rapid field assessment
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