Long-time fermentation has always been one of the reasons restricting the development of straw biological pulping.This study aimed to develop a novel straw pulp film with shortened solid-state fermentation time with l...Long-time fermentation has always been one of the reasons restricting the development of straw biological pulping.This study aimed to develop a novel straw pulp film with shortened solid-state fermentation time with less than 20%mass loss rate by bio-pulping synergistic treatment of straw fibers with deep eutectic solvent(DES)and Streptomyces rochei(S.rochei).Results illustrated that at 3%S.rochei concentration with 7-day fermentation,both cellulose and hemicellulose enzyme activities of the treated rice straw fiber reached peak values with a fiber mass loss rate of 17.01%.Microstructural morphology revealed that S.rochei colonization initiated on straw surfaces and progressively penetrated internal structures,resulting in surface loosening and distinct disruption of cell wall tissues within vascular bundles in transverse sections.The treated rice straw strip indicated a maximum tensile strength of 46.22 MPa for(Bacteria)BA 3%at day 7,attributed to optimized synergistic effects of microfibril angle(MFA)and cellulose/hemicellulose relative content ratio.The modified straw pulp film exhibited significant enhancement in the tensile index(44.9%increase),burst index(10.3%increase),and tear index(60%increase)compared to untreated groups.This work demonstrated the important role ofDES and S.rochei bio-pulping synergistic treatment in improving rice straw pulp performance,suggesting an eco-friendly,novel,and efficient biomass pretreatment technology for potential application prospects in sustainable agricultural mulching materials.展开更多
根据供试放线菌‘D74’菌株的菌落形态特征及16S r RNA基因序列分析,确定其为娄彻氏链霉菌(Streptomyces rochei)。采用皿内拮抗试验研究表明,‘D74’对魔芋软腐病菌有较强的拮抗作用,拮抗圈直径为11.0~18.3 mm。用‘D74’无菌发酵滤液1...根据供试放线菌‘D74’菌株的菌落形态特征及16S r RNA基因序列分析,确定其为娄彻氏链霉菌(Streptomyces rochei)。采用皿内拮抗试验研究表明,‘D74’对魔芋软腐病菌有较强的拮抗作用,拮抗圈直径为11.0~18.3 mm。用‘D74’无菌发酵滤液10倍稀释液处理甜瓜种子,其发芽率、简明活力指数、胚轴和胚根长度分别较对照增加34.9%、82.4%、66.4%和31.5%(P【0.05)。盆栽条件下,拌土单接菌剂D74和菌剂与病原菌混接处理对收获期魔芋的相对防效分别为100%和17.0%。菌剂D74在单接和混接条件下能提高盆栽魔芋叶片光合作用、叶片绿色度(SPAD)、生物量及产量,亦能改善魔芋球茎品质。田间小区试验条件下,菌剂D74穴施,魔芋叶片光合指标、生物量及产量较对照增加。研究表明,放线菌‘D74’对魔芋软腐病菌有较强抑制作用,‘D74’活菌制剂对魔芋有良好的防病促生效果,对魔芋品质也有显著的改善作用。展开更多
以苹果树腐烂病菌为靶标菌,通过对峙法和生长速率法对分离自苹果树根际土壤的放线菌进行筛选,对筛选出的拮抗菌株通过形态学和分子生物学特征进行鉴定,并测定了拮抗菌ZZ-9发酵滤液对苹果树腐烂病菌孢子萌发和菌丝生长的影响及离体枝条防...以苹果树腐烂病菌为靶标菌,通过对峙法和生长速率法对分离自苹果树根际土壤的放线菌进行筛选,对筛选出的拮抗菌株通过形态学和分子生物学特征进行鉴定,并测定了拮抗菌ZZ-9发酵滤液对苹果树腐烂病菌孢子萌发和菌丝生长的影响及离体枝条防效.结果表明:经对峙初筛,15株放线菌对苹果树腐烂病菌具有抑菌作用,占所分离株数的18.8%,其中抑制率>50%的有8株.复筛结果表明,ZZ-9对腐烂病菌抑制率最高,达96.4%,显著高于其他菌株;通过培养特征、生理生化特性及16S r DNA序列分析将菌株ZZ-9初步鉴定为娄彻氏链霉菌,其在Gen Bank上的序列登录号为KT986228;不同稀释倍数的ZZ-9发酵滤液对腐烂病孢子萌发及菌丝生长均有明显的抑制作用,其中50倍发酵滤液对孢子萌发抑制率和菌丝生长抑制率均达80%以上,且受抑制菌丝颜色加深,分支增多,末端膨大、畸形,出现原生质浓缩与释放现象;离体枝条防效试验表明,菌株ZZ-9发酵原液对苹果树腐烂病防效可达75%以上,表明该菌株可作为防治苹果树腐烂病的生防菌株.展开更多
基金funded by the Agricultural Science and Technology Innovation Fund of Jiangsu Province[Grant/Award Number:CX(24)1008]Key Research and Development Program Project of Henan Province(251111110100)National State Science Foundation of China[Grant/Award Number:21808093].
文摘Long-time fermentation has always been one of the reasons restricting the development of straw biological pulping.This study aimed to develop a novel straw pulp film with shortened solid-state fermentation time with less than 20%mass loss rate by bio-pulping synergistic treatment of straw fibers with deep eutectic solvent(DES)and Streptomyces rochei(S.rochei).Results illustrated that at 3%S.rochei concentration with 7-day fermentation,both cellulose and hemicellulose enzyme activities of the treated rice straw fiber reached peak values with a fiber mass loss rate of 17.01%.Microstructural morphology revealed that S.rochei colonization initiated on straw surfaces and progressively penetrated internal structures,resulting in surface loosening and distinct disruption of cell wall tissues within vascular bundles in transverse sections.The treated rice straw strip indicated a maximum tensile strength of 46.22 MPa for(Bacteria)BA 3%at day 7,attributed to optimized synergistic effects of microfibril angle(MFA)and cellulose/hemicellulose relative content ratio.The modified straw pulp film exhibited significant enhancement in the tensile index(44.9%increase),burst index(10.3%increase),and tear index(60%increase)compared to untreated groups.This work demonstrated the important role ofDES and S.rochei bio-pulping synergistic treatment in improving rice straw pulp performance,suggesting an eco-friendly,novel,and efficient biomass pretreatment technology for potential application prospects in sustainable agricultural mulching materials.
文摘根据供试放线菌‘D74’菌株的菌落形态特征及16S r RNA基因序列分析,确定其为娄彻氏链霉菌(Streptomyces rochei)。采用皿内拮抗试验研究表明,‘D74’对魔芋软腐病菌有较强的拮抗作用,拮抗圈直径为11.0~18.3 mm。用‘D74’无菌发酵滤液10倍稀释液处理甜瓜种子,其发芽率、简明活力指数、胚轴和胚根长度分别较对照增加34.9%、82.4%、66.4%和31.5%(P【0.05)。盆栽条件下,拌土单接菌剂D74和菌剂与病原菌混接处理对收获期魔芋的相对防效分别为100%和17.0%。菌剂D74在单接和混接条件下能提高盆栽魔芋叶片光合作用、叶片绿色度(SPAD)、生物量及产量,亦能改善魔芋球茎品质。田间小区试验条件下,菌剂D74穴施,魔芋叶片光合指标、生物量及产量较对照增加。研究表明,放线菌‘D74’对魔芋软腐病菌有较强抑制作用,‘D74’活菌制剂对魔芋有良好的防病促生效果,对魔芋品质也有显著的改善作用。
文摘以苹果树腐烂病菌为靶标菌,通过对峙法和生长速率法对分离自苹果树根际土壤的放线菌进行筛选,对筛选出的拮抗菌株通过形态学和分子生物学特征进行鉴定,并测定了拮抗菌ZZ-9发酵滤液对苹果树腐烂病菌孢子萌发和菌丝生长的影响及离体枝条防效.结果表明:经对峙初筛,15株放线菌对苹果树腐烂病菌具有抑菌作用,占所分离株数的18.8%,其中抑制率>50%的有8株.复筛结果表明,ZZ-9对腐烂病菌抑制率最高,达96.4%,显著高于其他菌株;通过培养特征、生理生化特性及16S r DNA序列分析将菌株ZZ-9初步鉴定为娄彻氏链霉菌,其在Gen Bank上的序列登录号为KT986228;不同稀释倍数的ZZ-9发酵滤液对腐烂病孢子萌发及菌丝生长均有明显的抑制作用,其中50倍发酵滤液对孢子萌发抑制率和菌丝生长抑制率均达80%以上,且受抑制菌丝颜色加深,分支增多,末端膨大、畸形,出现原生质浓缩与释放现象;离体枝条防效试验表明,菌株ZZ-9发酵原液对苹果树腐烂病防效可达75%以上,表明该菌株可作为防治苹果树腐烂病的生防菌株.
