In order to induce tetraploid into Jinjiang oyster (Crassostrea rivularis), embryos were treated with physical (cold or heat shock) and chemical (chlorpromazinum or traditional Chinese medicine)methods in the first 3 ...In order to induce tetraploid into Jinjiang oyster (Crassostrea rivularis), embryos were treated with physical (cold or heat shock) and chemical (chlorpromazinum or traditional Chinese medicine)methods in the first 3 min before the cleavage of zygotes or at the onset of the first cleavage of one or two eggs. Measurements show that the induction rates of tetraploid in the embryos inspected are 28%, 30%, 28. 4% and 35. 8% for heat-shock (39℃, 3 min),cold-shock (10℃, 3min),chlorpromazinum (0. 375 mg/dm3, 10 min) and traditional Chinese medicine (50 g/dm3 )extract (57 cm3, 10 min),respectively. The tetraploid induction rate in the larvae treated with traditional Chinese medicine is 32. 5%.It is evident that the induction rate of tetraploid in embryos insected at an early stage is the most remarkable when treated. with traditional Chinese medicine.展开更多
A study of the genetic diversity within four subpopulations of Chimonobambusa rivularis Yi and adaptations to altitude, habitat and related factors in Qionglai City of Sichuan province in China, analyzed by random amp...A study of the genetic diversity within four subpopulations of Chimonobambusa rivularis Yi and adaptations to altitude, habitat and related factors in Qionglai City of Sichuan province in China, analyzed by random amplified polymorphic DNA technique. 22 random primers were selected in the amplification and 375 repetitive loci with 350 polymorphic loci were produced. The total average percentage of amplification loci was 93.3%. The genetic diversity of every subpopulation was medium on （the percentage of amplification loci was 49.33%-66.67%）. The genetic diversity of high altitude samples of Zhengtiantai was lower than that of low altitude Wutonggang＇s. There was no obvious differentiation between two subpopulations of Zhengtiantais. The correlation is very low between altitude and the amplification loci and the genetic distance, Pearson correlation coefficient was 0.431 and 0.488 （P 〈 0.01）. Through investigation, the primary cause of heredity multiplicity drops was that the habitat had been destroyed by tourism development and other human disturbance.展开更多
文摘In order to induce tetraploid into Jinjiang oyster (Crassostrea rivularis), embryos were treated with physical (cold or heat shock) and chemical (chlorpromazinum or traditional Chinese medicine)methods in the first 3 min before the cleavage of zygotes or at the onset of the first cleavage of one or two eggs. Measurements show that the induction rates of tetraploid in the embryos inspected are 28%, 30%, 28. 4% and 35. 8% for heat-shock (39℃, 3 min),cold-shock (10℃, 3min),chlorpromazinum (0. 375 mg/dm3, 10 min) and traditional Chinese medicine (50 g/dm3 )extract (57 cm3, 10 min),respectively. The tetraploid induction rate in the larvae treated with traditional Chinese medicine is 32. 5%.It is evident that the induction rate of tetraploid in embryos insected at an early stage is the most remarkable when treated. with traditional Chinese medicine.
文摘A study of the genetic diversity within four subpopulations of Chimonobambusa rivularis Yi and adaptations to altitude, habitat and related factors in Qionglai City of Sichuan province in China, analyzed by random amplified polymorphic DNA technique. 22 random primers were selected in the amplification and 375 repetitive loci with 350 polymorphic loci were produced. The total average percentage of amplification loci was 93.3%. The genetic diversity of every subpopulation was medium on （the percentage of amplification loci was 49.33%-66.67%）. The genetic diversity of high altitude samples of Zhengtiantai was lower than that of low altitude Wutonggang＇s. There was no obvious differentiation between two subpopulations of Zhengtiantais. The correlation is very low between altitude and the amplification loci and the genetic distance, Pearson correlation coefficient was 0.431 and 0.488 （P 〈 0.01）. Through investigation, the primary cause of heredity multiplicity drops was that the habitat had been destroyed by tourism development and other human disturbance.
