目的:应用斑点追踪技术评价和比较左心衰伴有和不伴有肺动脉高压患者的右心室功能。方法:收集扩张型心肌病患者50名。根据超声所测得的肺动脉收缩压,分为无肺动脉高压组25名(SPAP<40 mm Hg),和合并肺动脉高压组25名(SPAP≥40 mm Hg)...目的:应用斑点追踪技术评价和比较左心衰伴有和不伴有肺动脉高压患者的右心室功能。方法:收集扩张型心肌病患者50名。根据超声所测得的肺动脉收缩压,分为无肺动脉高压组25名(SPAP<40 mm Hg),和合并肺动脉高压组25名(SPAP≥40 mm Hg)。右心室局部和整体的收缩功能用二维斑点追踪技术的多参数进行评价。结果 :与不合并肺动脉高压者相比,扩张型心肌病合并肺动脉高压者右心室的应变和三尖瓣环收缩期位移(TAPSE)均显著减低。TAPSE与肺动脉收缩压和左心室E/e比值呈负相关。结论:左心疾病相关性肺高压可以加重扩张型心肌病患者右心功能的损害。展开更多
Background Tetralogy of Fallot （TOF） is the most common malformation of children with an incidence of approximately 10% of congenital heart disease patients. There can be a wide spectrum to the severity of the anato...Background Tetralogy of Fallot （TOF） is the most common malformation of children with an incidence of approximately 10% of congenital heart disease patients. There can be a wide spectrum to the severity of the anatomic defects, which include ventricular septal defect, aortic override, right ventricular outflow tract obstruction, and right ventricular hypertrophy. We examined the relationship between right ventricular hypertrophy in patients with TOF and the gene expression of factors in the mitogen-activated protein kinase （MAPK） signal pathway. Methods To gain insight into the characteristic gene（s） involved in molecular mechanisms of right ventricular hypertrophy in TOF, differential mRNA and micro RNA expression profiles were assessed using expression-based micro array technology on right ventricular biopsies from young TOF patients who underwent primary correction and on normal heart tissue. We then analyzed the gene expression of the MAPK signal pathway using reverse transcription-polymerase chain reaction （RT-PCR） in normals and TOF patients. Results Using the micro RNA chip V3.0 and human whole genome oligonucleotide microarray VI.0 to detect the gene expression, we found 1068 genes showing altered expression of at least two-fold in TOF patients compared to the normal hearts, and 47 micro RNAs that showed a significant difference of at least two-fold in TOF patients. We then analyzed these mRNAs and micro RNAs by target gene predicting software Microcosm Targets version 5.0, and determined those mRNA highly relevant to the right ventricular hypertrophy by RT-PCR method. There were obvious differences in the gene expression of factors in the MAPK signal pathway when using RT-PCR, which was consistent to the results of the cDNA microarray.Conclusion The upregulation of genes in the MAPK signal pathway may be the key events that contribute to right ventricular hypertrophy and stunted angiogenesis in patients with TOF.展开更多
文摘目的:应用斑点追踪技术评价和比较左心衰伴有和不伴有肺动脉高压患者的右心室功能。方法:收集扩张型心肌病患者50名。根据超声所测得的肺动脉收缩压,分为无肺动脉高压组25名(SPAP<40 mm Hg),和合并肺动脉高压组25名(SPAP≥40 mm Hg)。右心室局部和整体的收缩功能用二维斑点追踪技术的多参数进行评价。结果 :与不合并肺动脉高压者相比,扩张型心肌病合并肺动脉高压者右心室的应变和三尖瓣环收缩期位移(TAPSE)均显著减低。TAPSE与肺动脉收缩压和左心室E/e比值呈负相关。结论:左心疾病相关性肺高压可以加重扩张型心肌病患者右心功能的损害。
文摘Background Tetralogy of Fallot （TOF） is the most common malformation of children with an incidence of approximately 10% of congenital heart disease patients. There can be a wide spectrum to the severity of the anatomic defects, which include ventricular septal defect, aortic override, right ventricular outflow tract obstruction, and right ventricular hypertrophy. We examined the relationship between right ventricular hypertrophy in patients with TOF and the gene expression of factors in the mitogen-activated protein kinase （MAPK） signal pathway. Methods To gain insight into the characteristic gene（s） involved in molecular mechanisms of right ventricular hypertrophy in TOF, differential mRNA and micro RNA expression profiles were assessed using expression-based micro array technology on right ventricular biopsies from young TOF patients who underwent primary correction and on normal heart tissue. We then analyzed the gene expression of the MAPK signal pathway using reverse transcription-polymerase chain reaction （RT-PCR） in normals and TOF patients. Results Using the micro RNA chip V3.0 and human whole genome oligonucleotide microarray VI.0 to detect the gene expression, we found 1068 genes showing altered expression of at least two-fold in TOF patients compared to the normal hearts, and 47 micro RNAs that showed a significant difference of at least two-fold in TOF patients. We then analyzed these mRNAs and micro RNAs by target gene predicting software Microcosm Targets version 5.0, and determined those mRNA highly relevant to the right ventricular hypertrophy by RT-PCR method. There were obvious differences in the gene expression of factors in the MAPK signal pathway when using RT-PCR, which was consistent to the results of the cDNA microarray.Conclusion The upregulation of genes in the MAPK signal pathway may be the key events that contribute to right ventricular hypertrophy and stunted angiogenesis in patients with TOF.
