Retrogradation of semi-dry rice noodles severely reduced their eating quality during storage.Three commonly used modified starches(oxidized starch,acetylated starch,and hydroxypropyl starch)were applied to investigate...Retrogradation of semi-dry rice noodles severely reduced their eating quality during storage.Three commonly used modified starches(oxidized starch,acetylated starch,and hydroxypropyl starch)were applied to investigate the anti-retrogradation effects of semi-dry rice noodles during cold storage.Loss of water content,migration of water,and increase in relative crystallinity,retrogradation enthalpy,and hardness could be retarded by adding chemically modified starches,especially hydroxypropyl starches.The effect of hydroxypropyl starch addition levels(2%,4%,6%,8%,and 10%)on the properties of rice flour and the edible quality of semi-dry rice noodles was further evaluated.The water solubility index of rice flour decreased with the addition of hydroxypropyl starch,while the swelling power showed the opposite trend.The quality of semi-dry rice noodles were improved with the addition of hydroxypropyl starch.Compared to the control,semi-dried rice noodles with 8%hydroxypropyl starch possessed superior properties,i.e.lower cooking loss(decreasing from 12.89%to 6.62%),lower adhesiveness(decreasing from 5.40 to 4.31 g·s),and higher hardness(rising from 10.89 to 13.81 N).These findings demonstrated that the incorporation of hydroxypropyl starch is a promising strategy for the preparation of semi-dry rice noodles with satisfactory cooking and edible qualities as well as a long shelf life.展开更多
Starch retrogradation is a primary contributor to the staling of bread.The impact of the surfactant glyceryl monostearate(GMS)on the structure and retrogradation characteristics of wheat starch gels was explored durin...Starch retrogradation is a primary contributor to the staling of bread.The impact of the surfactant glyceryl monostearate(GMS)on the structure and retrogradation characteristics of wheat starch gels was explored during storage at 4℃ for 2 h and 5 d.In this research,the incorporation of GMS(0.14%,W/W,based on flour weight)significantly reduced the crumb firmness(P<0.05).Molecular dynamics simulation vividly demonstrated the formation process of the single-helical amylose-GMS complex within 150 ns during the short-term retrogradation process of cooling the gelatinized starch for 2 h.X-ray diffraction analysis revealed that GMS slightly decreased the relative crystallinity of starch from 8.9%to 7.8%during long-term retrogradation.The reduction in R1047/1022 detected by Fourier-transform infrared spectroscopy indicated that GMS could reduce the degree of retrogradation.Solid-state ^(13)C NMR analysis showed a characteristic resonance peak at 31.7 ppm for the GMSstarch complex.This study indicates that GMS holds great application potential in retarding starch retrogradation.展开更多
The polysaccharides, such as κ-carrageenan, ι-carrageenan, agarose (agar), gellan gum, amylose, curdlan, alginate, and deacetylated rhamsan gum, in water changed into an ice-like structure with hydrogen bonding betw...The polysaccharides, such as κ-carrageenan, ι-carrageenan, agarose (agar), gellan gum, amylose, curdlan, alginate, and deacetylated rhamsan gum, in water changed into an ice-like structure with hydrogen bonding between polymer and water molecules, and between water-water molecules even at a concentration range of 0.1% - 1.0% (W/V) at room temperature, resulting in gelation. Such dramatic changes from liquid into gels have been understood at the molecular level in principles. In this review, we describe the structure-function relationship of starch on the view point of rheological aspects and discuss gelatinization and retrogradation mechanism including water molecules at molecular level. The starch molecules (amylose and amylopectin) play a dominant role in the center of the tetrahedral cavities occupied by water molecules, and the arrangement is partially similar to a tetrahedral structure in a gelatinization process. The arrangement should lead to a cooperative effect stabilizing extended regions of ice-like water with hydrogen bonding on the surface of the polymer molecules, where hemiacetal oxygen and hydroxyl groups might participate in hydrogen bonding with water molecules. Thus, a more extended ice-like hydrogen bonding within water molecules might be achieved in a retrogradation process. Though many investigations not only include starch gelatinization and retrogradaion, but also the gelling properties of the polysaccharides have been undertaken to elucidate the structure-function relationship, no other researchers have established mechanism at the molecular level. There is reasonable consistency in our investigations.展开更多
The heat intolerance and retrogradation behavior of native wheat starch(WS)are undesirable for starch-based gel foods,therefore the combinations of WS and hydrocolloids have attracted extensive attentions.The objectiv...The heat intolerance and retrogradation behavior of native wheat starch(WS)are undesirable for starch-based gel foods,therefore the combinations of WS and hydrocolloids have attracted extensive attentions.The objectives of this study were to investigate the effects of konjac glucomannan(KGM)with different degree of deacetylation(DD)on the pasting,rheological and retrogradation properties of WS.Results showed that deacetyl-konjac glucomannan(DKGM)increased the peak viscosity(PV),trough viscosity(TV)and final viscosity(FV),while decreased the breakdown(BD)values of WS,suggesting the enhancement of the viscosity and thermal stability of WS paste.The raised storage modulus(G′)of WS gels with DKGM1(DD,48.23%)demonstrated that the partial removal of acetyl groups from KGM chains effectively promoted the formation of gel network,accompanied by increased elasticity.After storing at 4℃ for 7 days,the hardness of gels increased up 369%,while supplement with DKGM significantly(p<0.05)decreased the hardness.These data demonstrated that DKGM delayed the process of starch retrogradation,which was further verified by LF-NMR analysis.Besides,the addition of DKGM reduced the retrogradation rate,relative crystallinity and the orderliness of WS-DKGM gels with the prolonging of storage at 4℃,all validating the hindrance of starch retrogradation by DKGM.Therefore,these data suggested that partial deacetylation of KGM could effectively improve the pasting,rheological characteristics and impede the retrogradation of WS.展开更多
To lower the retrogradation and digestibility of waxy corn starch for different food applications,a novel thermostable GtfC type 4,6-α-glucanotransferase without N-and C-terminals(GsGtfC)from Geobacillus sp.12AMOR1 w...To lower the retrogradation and digestibility of waxy corn starch for different food applications,a novel thermostable GtfC type 4,6-α-glucanotransferase without N-and C-terminals(GsGtfC)from Geobacillus sp.12AMOR1 was used.Waxy corn starch of 50 g/L was incubated with GsGtfC of 40-100 U/g substrate at 65℃and pH 5.5 for 1 h.Its molecular weight,iodine affinity,XRD crystallinity,and FTIR ratio of heights of bands at 1047 and 1022 cm^(-1) decreased,but ratio of DP<6 to DP≥25 branches and degree of branching increased.GsGtfC cleavedα-1,4-glycosidic bonds and inducedα-1,6-branching points to produce reuteran-likes polymers,which is different from Exiguobacterium sibiricum GtfC enzyme cleavingα-1,4-glycosidic bonds and synthesizing consecutiveα-1,6-glycosidic bonds to produce isomalto/malto-oligosaccharides.GsGtfC modified waxy corn starch had significantly lower DSC retrogradation enthalpies during the storage at 4℃for 3-14 days and significantly lower released glucose during the incubation with mammalian mucosalα-glucosidase at 37℃for 10-360 min.GsGtfC at 100 U/g substrate increased slowly digestible portion from 11.07%to 24.11%.展开更多
This letter reports a gravitational redshift measurement experiment using a satellite-based compact passive hydrogen maser(PHM)in a lunar distant retrograde orbit(DRO).In March 2024,the Chinese Academy of Sciences lau...This letter reports a gravitational redshift measurement experiment using a satellite-based compact passive hydrogen maser(PHM)in a lunar distant retrograde orbit(DRO).In March 2024,the Chinese Academy of Sciences launched the DRO-A/B twin satellites,which entered a DRO in July 2024.This orbit has a geocentric distance of approximately 300,000–450,000 kilometers and a 2:1 resonance ratio.Employing microwave dual one-way ranging(DOWR),satellite-ground time-frequency comparisons were successfully achieved in April 2025 using the PHM aboard the DRO-A satellite.This study validated the in-orbit performance of the compact PHM and supported tests of the Einstein Equivalence Principle.The gravitational redshift measurement result is(8.74±4.17)×10^(−3).As the world’s first fundamental physics experiment to deploy PHMs in a lunar DRO,this study provides significant new engineering approaches for testing gravitational theories in cislunar space.展开更多
BACKGROUND Post-endoscopic retrograde cholangiopancreatography pancreatitis(PEP)is a prevalent and potentially serious complication in patients undergoing endoscopic retrograde cholangiopancreatography.AIM To comprehe...BACKGROUND Post-endoscopic retrograde cholangiopancreatography pancreatitis(PEP)is a prevalent and potentially serious complication in patients undergoing endoscopic retrograde cholangiopancreatography.AIM To comprehensively assess the efficacy of indomethacin therapy in reducing PEP risk.METHODS We searched PubMed,EMBASE,Scopus,and Cochrane Library databases to identify randomized controlled trials(RCTs)that compared rectal indomethacin with a control group to prevent PEP.Duplicates were removed,and studies were included based on the established inclusion criteria.We used the Cochrane Collaboration’s tool to assess the risk of bias in the RCTs.A random-effects model was applied to produce pooled risk ratios(RRs)with 95%confidence intervals(CIs).RESULTS We included a total of 30 RCTs involving 16977 patients.Compared to the control group,rectal indomethacin showed comparable rates of overall PEP(PEP;RR=0.85,95%CI:0.69-1.04,I2=79%)with no statistically significant difference of RR in mild(RR=0.92,95%CI:0.74-1.14),moderate(RR=0.78,95%CI:0.59-1.02),or severe PEP(RR=1.12,95%CI:0.75-1.67).There was also no difference in cases of adverse events(RR=0.97,95%CI:0.69-1.35),abdominal pain(RR=1.14,95%CI:0.80-1.62),bleeding(RR=1.07,95%CI:0.70-1.63),or mortality(RR=0.86,95%CI:0.56-1.33)between the two groups.Subgroup analyses were also performed.CONCLUSION Rectal indomethacin appears to be safe and may offer benefit in selected high-risk patients,though findings should be interpreted with caution due to high heterogeneity.展开更多
Background:Biliary stent placement during endoscopic retrograde cholangiopancreatography(ERCP)is important for drainage in common bile duct(CBD)strictures,while the stent length is associated with many stent-related c...Background:Biliary stent placement during endoscopic retrograde cholangiopancreatography(ERCP)is important for drainage in common bile duct(CBD)strictures,while the stent length is associated with many stent-related complications.We aimed to develop an artificial intelligence(AI)model for stent length selection during ERCP.Methods:Images of the patients who underwent ERCP and were diagnosed with CBD strictures were collected.Training involved identifying and delineating the duodenoscope,CBD and guidewire,calculating the pixel distance of the target guidewire and determining the required biliary stent length based on the diameter of the duodenoscope.The performance of the model,accuracy for length calculation and the assistance for endoscopists were validated using the testing set.Results:A total of 794 images from 431 patients were included and data augmentation was conducted.The mean intersection over union(mIoU)for duodenoscope,CBD and guidewire were 90.46%,84.79%and 84.64%,respectively.The accuracy in identifying the strictures was 97.58%(121/124).The accuracy for stent length calculation achieved 85.95%(104/121)with an error margin of±1 cm.The mean absolute error(MAE)and mean relative error(MRE)of the AI model was 0.81 cm and 0.13,respectively.The AI model could reduce approximately 202 mGycm^(2)of the radiation exposure for each patient.It significantly improved both MAE and MRE for less experienced endoscopists(P=0.01 and P=0.02,respectively).Conclusions:The AI model could accurately identify duodenoscope,CBD and guidewire,enabling accurate strictures identification and stent length selection.展开更多
Endoscopic retrograde cholangiopancreatography(ERCP)in patients with surgically altered anatomy remains a challenging field in therapeutic endoscopy due to the complex anatomical reconstructions that limit access to t...Endoscopic retrograde cholangiopancreatography(ERCP)in patients with surgically altered anatomy remains a challenging field in therapeutic endoscopy due to the complex anatomical reconstructions that limit access to the biliary tree.Over the past two decades,device-assisted enteroscopy(DAE),including singleballoon,double-balloon,and motorized spiral enteroscopy,has expanded the feasibility of ERCP in this population,with overall technical success rates generally reported between 70%and 90%.Nevertheless,these techniques are technically demanding,time-consuming,and frequently affected by limited reach and unstable positioning.More recently,interventional endoscopic ultrasound(EUS)-guided procedures have emerged as highly effective alternatives,significantly improving clinical outcomes in selected patients,particularly in those with long-limb Roux-en-Y reconstructions where conventional methods are less effective.Percutaneous transhepatic biliary drainage continues to represent a valuable salvage option when endoscopic approaches fail,though it is associated with a greater burden of reinterventions and adverse events.This minireview provides a comprehensive overview of the main endoscopic strategies for biliary drainage in altered anatomy,focusing on technical considerations,efficacy,and safety profiles of DAE-assisted ERCP,EUS-guided interventions,and motorized systems.The evolving landscape of biliary drainage in this setting highlights the need for tailored treatment strategies,multidisciplinary collaboration,referral to high-volume centers,and further prospective studies to refine patient selection and optimize clinical outcomes.展开更多
To perform various functions in the body,skeletal muscle is controlled and coordinated as a whole by nerves.However,there has been little research into whether the nerve control characteristics of different muscles ar...To perform various functions in the body,skeletal muscle is controlled and coordinated as a whole by nerves.However,there has been little research into whether the nerve control characteristics of different muscles are different,and the importance of these potential differences.In the present study,we used a three-dimensional imaging of solvent-cleared organ-compatible multi-tracer technique to explore the spatial distribution patterns of sensory and sympathetic neurons that innervate limb muscles.We integrated transcriptome sequencing datasets from mouse limb muscles in public databases and performed correlation analysis with neuronal spatial distribution data to reveal the unique effects of different types of neurons on muscle functional pathways.In terms of spatial distribution patterns,sympathetic neurons exhibited a more concentrated distribution than sensory and motor neurons.In addition,the neuronal innervation of limb muscles exhibited four different characteristics:sympathetic neuron-rich muscle,sensory neuron-rich muscle,neuron-sparse muscle,and motor neuron-rich muscle.Sensory neuron density was mainly associated with muscle contractile structure and cell pH,whereas sympathetic neuron density was associated with protein kinase activity,muscle vasculature,muscle calcium-dependent protein kinase activity,lipid transport,and vesicle release.Motor neuron density was mainly associated with protein kinase activity,cell adhesion,oxidoreductase activity,and exocytosis.These findings may contribute to a deeper understanding of how nerves cooperate to endow muscles with diverse physiological functions,thereby providing new insights and experimental evidence for the treatment of various neuromuscular diseases.展开更多
Aging is characterized by a decreased autophagic activity contributing to the intracellular deposition of damaged organelles and macromolecules.Autophagy is particularly challenging in neurons since autophagic vesicle...Aging is characterized by a decreased autophagic activity contributing to the intracellular deposition of damaged organelles and macromolecules.Autophagy is particularly challenging in neurons since autophagic vesicles are formed at the axonal tip and must be transported to the soma where final degradation occurs.Here,we examined if axonal transport of autophagic vesicles is altered during aging.We employed two-photon microscopy for in vivo imaging in the optic nerve of young and aged rats.In old animals(>18 months old),retrograde autophagic vesicle transport was significantly reduced with regard to motility and velocity.While activation of autophagy was decreased,expression of key proteins of the autophagy-lysosomal pathway including p62 and procathepsin D and the number of autophagolysosomes was increased.Maturation of autophagic vesicles was shifted to more distal regions of the axon and axonal lysosomal clearing was impaired.In a pull-down assay,the protein binding between dynein and dynactin was decreased by half,which could explain the retrograde axonal transport effects.Taken together,retrograde axonal autophagic vesicle transport in vivo is diminished during aging accompanied by decreased autophagy activation,alterations of the lysosomal pathway,and a reduced dynein-dynactin binding.展开更多
Avulsion injury of one or more spinal ventral roots induces a critical loss of motoneurons,followed by irreversible locomotor function impairment ranging from inadequate limb movement to complete paralysis of the limb...Avulsion injury of one or more spinal ventral roots induces a critical loss of motoneurons,followed by irreversible locomotor function impairment ranging from inadequate limb movement to complete paralysis of the limb.Recent surgical techniques facilitate improvement of limb function,but it remains to be determined exactly how many motoneurons are needed to survive and grow new axons to achieve sufficient muscle reinnervation.The aim of this study was to determine the minimum motoneuron quantity required to reinnervate the denervated skeletal muscles of the limb and produce a functionally satisfactory locomotor pattern.Since none of the commercially available methods and equipment were able to provide a quantifiable and in-depth analysis of the motor pattern of the entire hind limb,we have developed and applied a sensitive movement recording and analyzing system in order to determine the threshold of satisfactory functional reinnervation;we combined video-based footprint analysis and hind limb motion analysis to achieve a new and reliable assessment.Sprague-Dawley rats underwent a lumbar 4-5 ventral root avulsion,and their L4 ventral roots were subsequently reimplanted.The animals received different doses of riluzole treatment in order to rescue incremental numbers of the damaged motoneuron pool.We were able to assess one rear-view and six lateral parameters of the hind limb movement pattern by measuring specific joint angles,footprint,and gait parameters in single video frames.Four months after the operation,we performed Fast Blue retrograde tracing to label and count the reinnervating motoneurons.We then compared the numbers of reinnervating motoneurons and the functional improvement.Our results confirmed a strong relationship between functional restoration of the original movement pattern and morphological reinnervation;approximately 30%of the original motor pool was able to produce a useful locomotor pattern.We believe that our knowledge of the minimal motoneuron numbers required to reinnervate target muscles may help plan the segmental redistribution of the motoneuron pools for reinnervation surgeries.展开更多
Hydrophilic colloid-based edible curcumin nanoemulsion coatings was exploited to keep fried rice stabilization in the freeze-thaw cycles(FTCs),including texture,moisture loss,color and other aspects of the quality cha...Hydrophilic colloid-based edible curcumin nanoemulsion coatings was exploited to keep fried rice stabilization in the freeze-thaw cycles(FTCs),including texture,moisture loss,color and other aspects of the quality characteristics.Fried rice were coated with nanoemulsion what was obtained by flaxseed gum(FG),egg white protein(EWP),Olive oil(O),and Curcumin(Cur),namely as CFEO.The scanning electron microscopy(SEM)results showed that the CFEO nanoemulsion coating had a more homogeneous and dense surface structure.In the fried rice quality determination,the hardness of the CFEO nanoemulsion coating was significantly less than the CONTROL group in each cycle.Alternatively,for moisture loss,CONTROL group significantly accelerating the rate of loss.For the color index,the CFEO group had outstanding performance in delaying the increase of the total color difference(ΔE)and brown index(BI),additionally,significantly delayed the decline in whiteness index(WI).Retrogradation enthalpy,crystallinity and short range order analysis demonstrated the contribution of the CFEO group in the delayed starch retrogradation,and further assisted in explaining the changes in texture properties.In addition,the CFEO also played a stabilizing role in preventing the fluctuation of the T_(2) water distribution.Therefore,curcumin nanoemulsion as the coating of fried rice is appropriate and can delay the decline of quality in freeze-thaw cycle.展开更多
In the present study,we investigated the effects of sourdough on the staling rate of bread from the perspectives of starch retrogradation during storage and gluten depolymerization in bread dough.Three sourdough types...In the present study,we investigated the effects of sourdough on the staling rate of bread from the perspectives of starch retrogradation during storage and gluten depolymerization in bread dough.Three sourdough types,which were fermented using Weissella cibaria,Latilactobacillus sakei,and Fructilactobacillus sanfranciscensis strains,were added to bread dough at a 25%ratio.Compared with regular bread,sourdough bread(SDB)exhibited a lower hardness value and slower staling rate during a 7-day storage period.The relative crystallinities and enthalpies revealed that sourdough addition inhibited amylopectin recrystallization.Furthermore,sourdough-induced acid conditions promoted glutenin macropolymer depolymerization;and the dough containing sourdough(SD-D)showed a decrease in viscoelastic properties and changes on microstructure,reflecting a weakened gluten network structure.Among the three sourdough types,L.sakei-fermented sourdough exhibited the lowest staling rate;this might be owing to its higher gluten depolymerization degree,lower α-helix and intermolecular β-sheet contents,and decreased viscoelastic properties.Our study findings provide a theoretical basis for using sourdough fermentation with different strains to develop clean label bread and improve bread quality in the future.展开更多
BACKGROUND Endoscopic retrograde cholangiopancreatography(ERCP)is an invasive endoscopic procedure used mainly to treat hepato-pancreato-biliary(HPB)diseases.The need for ERCP has increased with the rising number of H...BACKGROUND Endoscopic retrograde cholangiopancreatography(ERCP)is an invasive endoscopic procedure used mainly to treat hepato-pancreato-biliary(HPB)diseases.The need for ERCP has increased with the rising number of HPB diseases over the past decade.Thus,due to increased demand,ERCP is performed at more centers.Currently,it is performed by general surgeons,gastroenterology and invasive radiology specialists in the United States and Europe as recommended by the British Society of Gastroenterology(BSG).AIM To present the results of ERCP procedures from fourteen surgical centers in Türkiye.METHODS Fourteen surgical centers performing ERCP were included in the present study.The age,gender,ERCP indication,success status,post-ERCP complications,ERCP reports and the files of 66993 patients who underwent ERCP were collected from the participating centers.The results are discussed according to the targets declared by the BSG,which are volume load per annum,proportion of successful cannulation(>85%),bile duct clearance rate(>75%),stenting rate for strictures(>80%)and complications(<6%).RESULTS A total of 66993 ERCP procedures were performed in the centers included in the study up to August 2024.29250(43.6%)of the procedures were performed urgently,especially for suppurative cholangitis,biliary tract injuries,etc.The remaining 37743(56.4%)cases were performed electively.50.2%of the patients were female and 49.8%were male.The average ages were 56.5 years for women and 55.9 years for men.General anesthesia was used in 84.1%of the patients while sedation was used in 15.9%.The indications were bile duct stone(78.7%),pancreatic tumor(3.9%),papillary tumor(3.3%),cholangiocarcinoma(2.6%),Oddi sphincter dysfunction(2.4%),bile leakage after cholecystectomy(2%),bile leakage after hydatid cyst surgery(1.9%),biliary stricture(1.7%),and other diseases(3.1%).Hyperamylasemia and post-ERCP pancreatitis were the most common complications as observed in 8.1%of the patients.They were usually self-limited and responded to supportive measures.The frequency of the other complications was also consistent with the literature.CONCLUSION There is a huge shortage of ERCP endoscopists worldwide due to insufficient ERCP training and centers especially in developing and underdeveloped countries.As patients requiring ERCP usually present to surgical practitioners,the incorporation of surgeons into this training program is an effective and reliable solution.The BSG recommends the incorporation of surgeons and radiologists in addition to gastroenterology specialists.This study is the first to present the results of ERCP procedures from fourteen surgical centers throughout Türkiye.The results suggest that the surgical centers included were able to achieve the targets set by the BSG.This study demonstrated that the surgical ERCP units in the present work have reached satisfactory results and provided a reliable and successful ERCP service.There are currently no issues regarding the validity and appropriateness of the surgeons performing ERCP.Therefore,ERCP training should be encouraged in surgeons and more surgical ERCP centers should be provided.展开更多
Background:The choice of a basket or a balloon catheter during endoscopic retrograde cholangiopancreatography(ERCP)for the clearance of pancreatic duct stones in patients with chronic pancreatitis(CP)remains controver...Background:The choice of a basket or a balloon catheter during endoscopic retrograde cholangiopancreatography(ERCP)for the clearance of pancreatic duct stones in patients with chronic pancreatitis(CP)remains controversial.This study compared the efficacy and safety of these two devices for pancreatic duct stone extractions.Methods:We compared the efficacy and safety of basket and balloon catheters for pancreatic stone extractions.We enrolled CP patients who underwent ERCP for the first time at Changhai Hospital,Naval Medical University between February 2012 and December 2021.After propensity score matching(1:1),101 patients were included in each group.The primary outcome was the rate of pancreatic stone clearance.Secondary outcomes included the rate of adverse events during hospitalization,long-term pain relief,and quality of life after one year follow-up period.Results:The rate of complete clearance was comparable between the two groups(86.1%vs.84.2%,P=0.692).In patients with stones≥2 cm before extracorporeal shock wave lithotripsy(ESWL),the rate of complete clearance was significantly higher in the balloon catheter group when compared to the basket catheter group[100%(19/19)vs.70.0%(14/20),P=0.031].In the multivariate logistic analysis,ESWL prior to stone extraction was the only independent predictor of complete clearance[with ESWL 58.4%(264/452)vs.without ESWL 41.6%(188/452),odds ratio=2.3,95%confidence interval:1.2–4.3;P=0.013].No significant differences between groups were found regarding the rates of adverse events during hospitalization,quality of life,and pain relief after one year of follow-up.Conclusions:Basket and balloon catheters showed similar efficacy and safety for pancreatic stone extractions.However,the balloon catheter was superior to the basket catheter if the pancreatic stone size was≥2 cm before ESWL.展开更多
Biliary leaks can arise as a consequence of cholecystectomy,liver surgery,liver transplant,or,less frequently,trauma.Early identification and characterization of these leaks are crucial,as they can significantly enhan...Biliary leaks can arise as a consequence of cholecystectomy,liver surgery,liver transplant,or,less frequently,trauma.Early identification and characterization of these leaks are crucial,as they can significantly enhance patient outcomes by reducing morbidity and mortality.Traditionally,surgical repair has been the standard treatment;however,advancements in endoscopic techniques and tools have established endoscopic retrograde cholangiopancreatography(ERCP)as the primary approach for managing these often-complicated cases.Interventions such as sphincterotomy,nasobiliary drainage,and stent placement aim to alleviate the pressure within the bile duct,facilitating depressurization and promoting leak healing.Alongside ERCP,endoscopic ultrasound is playing an increasingly vital role in addressing challenging cases.Ongoing improvements in endoscopic technologies and methodologies offer promising prospects,often minimizing the need for invasive surgical interventions.Nonetheless,the management of biliary leaks continues to pose significant challenges for clinicians.An optimal approach for patients experiencing bile leakage should be determined on a case-by-case basis and discussed within a multidisciplinary team involving radiologists,endoscopists,and surgeons.This comprehensive review aims to elucidate the role of endoscopy in the management of various types of biliary leaks,providing clinicians with practical insights to navigate this complex field.展开更多
Implementing the flyby to Near-Earth Asteroids (NEAs) with the potential impact risks to the Earth allows for obtaining detailed physical parameters, thereby supporting the high-precision orbit prediction and planetar...Implementing the flyby to Near-Earth Asteroids (NEAs) with the potential impact risks to the Earth allows for obtaining detailed physical parameters, thereby supporting the high-precision orbit prediction and planetary defense strategy. Different from those conducted asteroid flyby missions, in the 12th China Trajectory Optimization Competition (CTOC-12), a NEAs flyby trajectory design problem using reusable probes that depart from a Lunar Distant Retrograde Orbit (DRO) station in the cislunar space was released. The objective was flyby to as many NEAs as possible using up to 20 probes within a total of 10 years. The ∑ team proposed a solution that can explore 47 NEAs using 11 probes, ranking the first in the competition. In this paper, the methods and results from the winning team are introduced, including mission analysis and preliminary design, and low-energy transfer trajectory optimization. In particular, a round-trip trajectory is divided into three phases: deep space transfer, indirect transfer between the Earth to DRO, and DRO phasing and rendezvous. With the combination of global optimization and local optimization algorithms, the required velocity increments to change the orbital planes are effectively reduced, thus increasing the number of the explored NEAs. The final solution of our team is presented and the results are compared with those of the top three teams. The competition demonstrates that the regularization of flyby missions from the cislunar space to explore NEAs with the potential impact risks to the Earth is the feasible and promising.展开更多
BACKGROUND Pancreaticobiliary maljunction(PBM)is a rare congenital abnormality in pancreaticobiliary duct development.PBM is commonly found in children,and it often leads to acute pancreatitis and other diseases as a ...BACKGROUND Pancreaticobiliary maljunction(PBM)is a rare congenital abnormality in pancreaticobiliary duct development.PBM is commonly found in children,and it often leads to acute pancreatitis and other diseases as a result of pancreaticobiliary reflux.Roux-en-Y choledochojejunostomy is a common surgical method for the treatment of PBM,but there are several associated complications that may occur after this operation.CASE SUMMARY The patient,a 12-year-old female,was hospitalized nearly 20 times in 2021 for recurrent acute pancreatitis.In 2022,she was diagnosed with PBM and underwent laparoscopic common bile duct resection and Roux-en-Y choledochojejunostomy in a tertiary hospital.In the first year after surgery,the patient had more than 10 recurrent acute pancreatitis episodes.After undergoing abdominal computed tomography and other examinations,she was diagnosed with“residual bile duct stones and recurrent acute pancreatitis”.On January 30,2024,the patient was admitted to our hospital due to recurrent upper abdominal pain and was cured through endoscopic retrograde cholangiopancreatography.CONCLUSION This article reports a case of a child with distal residual common bile duct stones and recurrent acute pancreatitis after Roux-en-Y choledochojejunostomy for PBM.The patient was cured through endoscopic retrograde cholangiopancreatography.展开更多
BACKGROUND Common bile duct stones pose a high risk of recurrence or disease progression if not promptly treated.However,there is still no optimal treatment approach.AIM To investigate the clinical efficacy of modifie...BACKGROUND Common bile duct stones pose a high risk of recurrence or disease progression if not promptly treated.However,there is still no optimal treatment approach.AIM To investigate the clinical efficacy of modified pancreatic duct stent drainage in endoscopic retrograde cholangiopancreatography(ERCP)for treating common bile duct stones.METHODS This retrospective study included 175 patients with common bile duct stones treated at Taizhou Fourth People’s Hospital between January 1,2021,and November 30,2023.The patients were divided into three groups-the modified pancreatic duct stent drainage group(59 cases),the nasobiliary drainage group(58 cases),and the standard biliary drainage group(58 cases).Preoperative general clinical data,laboratory indicators,and the visual analog scale(VAS)at two time points(24 hours before and after surgery)were compared,along with postoperative complications across the three groups.RESULTS Serum levels of aspartate aminotransferase,alanine aminotransferase,alkaline phosphatase,gamma-glutamyltransferase,total bilirubin,direct bilirubin,Creactive protein,and amylase were significantly lower in the modified pancreatic duct stent drainage group and the standard biliary drainage group than those in the nasobiliary drainage group(P<0.05).However,no statistically significant differences were observed in white blood cells,hemoglobin,or neutrophil levels among the three groups(P>0.05).The standard biliary drainage group had significantly lower VAS scores[(4.36±1.18)points]than those for the modified pancreatic duct stent drainage group[(4.92±1.68)points](P=0.033),and the nasobiliary drainage group[(5.54±1.24)points](P=0.017).There were no statistically significant differences in complication rates across the three groups(P>0.05).CONCLUSION Compared to standard biliary drainage and nasobiliary drainage,the modified pancreatic duct stent used during ERCP for patients with bile duct stones significantly reduced hepatocyte injury,improved liver function parameters,alleviated inflammation and pain,enhanced patient comfort,and demonstrated superior safety.展开更多
基金funded by the fund of national natural science foundation of China(32101996,32160530)the training plan for academic and technical leaders of major disciplines in Jiangxi province(20232BCJ23024)+1 种基金the key research and development program of Jiangxi province(20232BBF60023)“Shuangqian Project”of Jiangxi Province(jxsq2023201042)。
文摘Retrogradation of semi-dry rice noodles severely reduced their eating quality during storage.Three commonly used modified starches(oxidized starch,acetylated starch,and hydroxypropyl starch)were applied to investigate the anti-retrogradation effects of semi-dry rice noodles during cold storage.Loss of water content,migration of water,and increase in relative crystallinity,retrogradation enthalpy,and hardness could be retarded by adding chemically modified starches,especially hydroxypropyl starches.The effect of hydroxypropyl starch addition levels(2%,4%,6%,8%,and 10%)on the properties of rice flour and the edible quality of semi-dry rice noodles was further evaluated.The water solubility index of rice flour decreased with the addition of hydroxypropyl starch,while the swelling power showed the opposite trend.The quality of semi-dry rice noodles were improved with the addition of hydroxypropyl starch.Compared to the control,semi-dried rice noodles with 8%hydroxypropyl starch possessed superior properties,i.e.lower cooking loss(decreasing from 12.89%to 6.62%),lower adhesiveness(decreasing from 5.40 to 4.31 g·s),and higher hardness(rising from 10.89 to 13.81 N).These findings demonstrated that the incorporation of hydroxypropyl starch is a promising strategy for the preparation of semi-dry rice noodles with satisfactory cooking and edible qualities as well as a long shelf life.
基金supported by the Zhejiang Provincial Natural Science Foundation of China(LQ23C200003)National Natural Science Foundation of China(32202076)Natural Science Foundation of Shandong Province(Project No.ZR2023QC083).
文摘Starch retrogradation is a primary contributor to the staling of bread.The impact of the surfactant glyceryl monostearate(GMS)on the structure and retrogradation characteristics of wheat starch gels was explored during storage at 4℃ for 2 h and 5 d.In this research,the incorporation of GMS(0.14%,W/W,based on flour weight)significantly reduced the crumb firmness(P<0.05).Molecular dynamics simulation vividly demonstrated the formation process of the single-helical amylose-GMS complex within 150 ns during the short-term retrogradation process of cooling the gelatinized starch for 2 h.X-ray diffraction analysis revealed that GMS slightly decreased the relative crystallinity of starch from 8.9%to 7.8%during long-term retrogradation.The reduction in R1047/1022 detected by Fourier-transform infrared spectroscopy indicated that GMS could reduce the degree of retrogradation.Solid-state ^(13)C NMR analysis showed a characteristic resonance peak at 31.7 ppm for the GMSstarch complex.This study indicates that GMS holds great application potential in retarding starch retrogradation.
文摘The polysaccharides, such as κ-carrageenan, ι-carrageenan, agarose (agar), gellan gum, amylose, curdlan, alginate, and deacetylated rhamsan gum, in water changed into an ice-like structure with hydrogen bonding between polymer and water molecules, and between water-water molecules even at a concentration range of 0.1% - 1.0% (W/V) at room temperature, resulting in gelation. Such dramatic changes from liquid into gels have been understood at the molecular level in principles. In this review, we describe the structure-function relationship of starch on the view point of rheological aspects and discuss gelatinization and retrogradation mechanism including water molecules at molecular level. The starch molecules (amylose and amylopectin) play a dominant role in the center of the tetrahedral cavities occupied by water molecules, and the arrangement is partially similar to a tetrahedral structure in a gelatinization process. The arrangement should lead to a cooperative effect stabilizing extended regions of ice-like water with hydrogen bonding on the surface of the polymer molecules, where hemiacetal oxygen and hydroxyl groups might participate in hydrogen bonding with water molecules. Thus, a more extended ice-like hydrogen bonding within water molecules might be achieved in a retrogradation process. Though many investigations not only include starch gelatinization and retrogradaion, but also the gelling properties of the polysaccharides have been undertaken to elucidate the structure-function relationship, no other researchers have established mechanism at the molecular level. There is reasonable consistency in our investigations.
基金This study was supported by the National Key Research and Development Program of China during the 13th Five-Year Plan(2018YFD0400501)Key Research and development plan for Colleges and Universities in Henan Province(20A550013).
文摘The heat intolerance and retrogradation behavior of native wheat starch(WS)are undesirable for starch-based gel foods,therefore the combinations of WS and hydrocolloids have attracted extensive attentions.The objectives of this study were to investigate the effects of konjac glucomannan(KGM)with different degree of deacetylation(DD)on the pasting,rheological and retrogradation properties of WS.Results showed that deacetyl-konjac glucomannan(DKGM)increased the peak viscosity(PV),trough viscosity(TV)and final viscosity(FV),while decreased the breakdown(BD)values of WS,suggesting the enhancement of the viscosity and thermal stability of WS paste.The raised storage modulus(G′)of WS gels with DKGM1(DD,48.23%)demonstrated that the partial removal of acetyl groups from KGM chains effectively promoted the formation of gel network,accompanied by increased elasticity.After storing at 4℃ for 7 days,the hardness of gels increased up 369%,while supplement with DKGM significantly(p<0.05)decreased the hardness.These data demonstrated that DKGM delayed the process of starch retrogradation,which was further verified by LF-NMR analysis.Besides,the addition of DKGM reduced the retrogradation rate,relative crystallinity and the orderliness of WS-DKGM gels with the prolonging of storage at 4℃,all validating the hindrance of starch retrogradation by DKGM.Therefore,these data suggested that partial deacetylation of KGM could effectively improve the pasting,rheological characteristics and impede the retrogradation of WS.
基金This work was sponsored in part by National Natural Science Foundation of China(NSFC-31671801,31371749)the key R&D program(21ZGN38)from Changchun science and technology bureau,the special project of industrial independent innovation capability(2020C036-7)from Jilin provincial development and reform commission+1 种基金the science and technology research planning project(JJKH20220609KJ)from Jilin provincial department of educationthe scholar climbing project(ZKP202006 and ZKP202016)from Changchun University.
文摘To lower the retrogradation and digestibility of waxy corn starch for different food applications,a novel thermostable GtfC type 4,6-α-glucanotransferase without N-and C-terminals(GsGtfC)from Geobacillus sp.12AMOR1 was used.Waxy corn starch of 50 g/L was incubated with GsGtfC of 40-100 U/g substrate at 65℃and pH 5.5 for 1 h.Its molecular weight,iodine affinity,XRD crystallinity,and FTIR ratio of heights of bands at 1047 and 1022 cm^(-1) decreased,but ratio of DP<6 to DP≥25 branches and degree of branching increased.GsGtfC cleavedα-1,4-glycosidic bonds and inducedα-1,6-branching points to produce reuteran-likes polymers,which is different from Exiguobacterium sibiricum GtfC enzyme cleavingα-1,4-glycosidic bonds and synthesizing consecutiveα-1,6-glycosidic bonds to produce isomalto/malto-oligosaccharides.GsGtfC modified waxy corn starch had significantly lower DSC retrogradation enthalpies during the storage at 4℃for 3-14 days and significantly lower released glucose during the incubation with mammalian mucosalα-glucosidase at 37℃for 10-360 min.GsGtfC at 100 U/g substrate increased slowly digestible portion from 11.07%to 24.11%.
文摘This letter reports a gravitational redshift measurement experiment using a satellite-based compact passive hydrogen maser(PHM)in a lunar distant retrograde orbit(DRO).In March 2024,the Chinese Academy of Sciences launched the DRO-A/B twin satellites,which entered a DRO in July 2024.This orbit has a geocentric distance of approximately 300,000–450,000 kilometers and a 2:1 resonance ratio.Employing microwave dual one-way ranging(DOWR),satellite-ground time-frequency comparisons were successfully achieved in April 2025 using the PHM aboard the DRO-A satellite.This study validated the in-orbit performance of the compact PHM and supported tests of the Einstein Equivalence Principle.The gravitational redshift measurement result is(8.74±4.17)×10^(−3).As the world’s first fundamental physics experiment to deploy PHMs in a lunar DRO,this study provides significant new engineering approaches for testing gravitational theories in cislunar space.
文摘BACKGROUND Post-endoscopic retrograde cholangiopancreatography pancreatitis(PEP)is a prevalent and potentially serious complication in patients undergoing endoscopic retrograde cholangiopancreatography.AIM To comprehensively assess the efficacy of indomethacin therapy in reducing PEP risk.METHODS We searched PubMed,EMBASE,Scopus,and Cochrane Library databases to identify randomized controlled trials(RCTs)that compared rectal indomethacin with a control group to prevent PEP.Duplicates were removed,and studies were included based on the established inclusion criteria.We used the Cochrane Collaboration’s tool to assess the risk of bias in the RCTs.A random-effects model was applied to produce pooled risk ratios(RRs)with 95%confidence intervals(CIs).RESULTS We included a total of 30 RCTs involving 16977 patients.Compared to the control group,rectal indomethacin showed comparable rates of overall PEP(PEP;RR=0.85,95%CI:0.69-1.04,I2=79%)with no statistically significant difference of RR in mild(RR=0.92,95%CI:0.74-1.14),moderate(RR=0.78,95%CI:0.59-1.02),or severe PEP(RR=1.12,95%CI:0.75-1.67).There was also no difference in cases of adverse events(RR=0.97,95%CI:0.69-1.35),abdominal pain(RR=1.14,95%CI:0.80-1.62),bleeding(RR=1.07,95%CI:0.70-1.63),or mortality(RR=0.86,95%CI:0.56-1.33)between the two groups.Subgroup analyses were also performed.CONCLUSION Rectal indomethacin appears to be safe and may offer benefit in selected high-risk patients,though findings should be interpreted with caution due to high heterogeneity.
基金supported by grants from the Taishan Scholars Program of Shandong Province(tsqn202312333)the National Natural Science Foundation of China(82470695).
文摘Background:Biliary stent placement during endoscopic retrograde cholangiopancreatography(ERCP)is important for drainage in common bile duct(CBD)strictures,while the stent length is associated with many stent-related complications.We aimed to develop an artificial intelligence(AI)model for stent length selection during ERCP.Methods:Images of the patients who underwent ERCP and were diagnosed with CBD strictures were collected.Training involved identifying and delineating the duodenoscope,CBD and guidewire,calculating the pixel distance of the target guidewire and determining the required biliary stent length based on the diameter of the duodenoscope.The performance of the model,accuracy for length calculation and the assistance for endoscopists were validated using the testing set.Results:A total of 794 images from 431 patients were included and data augmentation was conducted.The mean intersection over union(mIoU)for duodenoscope,CBD and guidewire were 90.46%,84.79%and 84.64%,respectively.The accuracy in identifying the strictures was 97.58%(121/124).The accuracy for stent length calculation achieved 85.95%(104/121)with an error margin of±1 cm.The mean absolute error(MAE)and mean relative error(MRE)of the AI model was 0.81 cm and 0.13,respectively.The AI model could reduce approximately 202 mGycm^(2)of the radiation exposure for each patient.It significantly improved both MAE and MRE for less experienced endoscopists(P=0.01 and P=0.02,respectively).Conclusions:The AI model could accurately identify duodenoscope,CBD and guidewire,enabling accurate strictures identification and stent length selection.
文摘Endoscopic retrograde cholangiopancreatography(ERCP)in patients with surgically altered anatomy remains a challenging field in therapeutic endoscopy due to the complex anatomical reconstructions that limit access to the biliary tree.Over the past two decades,device-assisted enteroscopy(DAE),including singleballoon,double-balloon,and motorized spiral enteroscopy,has expanded the feasibility of ERCP in this population,with overall technical success rates generally reported between 70%and 90%.Nevertheless,these techniques are technically demanding,time-consuming,and frequently affected by limited reach and unstable positioning.More recently,interventional endoscopic ultrasound(EUS)-guided procedures have emerged as highly effective alternatives,significantly improving clinical outcomes in selected patients,particularly in those with long-limb Roux-en-Y reconstructions where conventional methods are less effective.Percutaneous transhepatic biliary drainage continues to represent a valuable salvage option when endoscopic approaches fail,though it is associated with a greater burden of reinterventions and adverse events.This minireview provides a comprehensive overview of the main endoscopic strategies for biliary drainage in altered anatomy,focusing on technical considerations,efficacy,and safety profiles of DAE-assisted ERCP,EUS-guided interventions,and motorized systems.The evolving landscape of biliary drainage in this setting highlights the need for tailored treatment strategies,multidisciplinary collaboration,referral to high-volume centers,and further prospective studies to refine patient selection and optimize clinical outcomes.
基金supported by the National Natural Science Foundation of China,No.82072162(to XY).
文摘To perform various functions in the body,skeletal muscle is controlled and coordinated as a whole by nerves.However,there has been little research into whether the nerve control characteristics of different muscles are different,and the importance of these potential differences.In the present study,we used a three-dimensional imaging of solvent-cleared organ-compatible multi-tracer technique to explore the spatial distribution patterns of sensory and sympathetic neurons that innervate limb muscles.We integrated transcriptome sequencing datasets from mouse limb muscles in public databases and performed correlation analysis with neuronal spatial distribution data to reveal the unique effects of different types of neurons on muscle functional pathways.In terms of spatial distribution patterns,sympathetic neurons exhibited a more concentrated distribution than sensory and motor neurons.In addition,the neuronal innervation of limb muscles exhibited four different characteristics:sympathetic neuron-rich muscle,sensory neuron-rich muscle,neuron-sparse muscle,and motor neuron-rich muscle.Sensory neuron density was mainly associated with muscle contractile structure and cell pH,whereas sympathetic neuron density was associated with protein kinase activity,muscle vasculature,muscle calcium-dependent protein kinase activity,lipid transport,and vesicle release.Motor neuron density was mainly associated with protein kinase activity,cell adhesion,oxidoreductase activity,and exocytosis.These findings may contribute to a deeper understanding of how nerves cooperate to endow muscles with diverse physiological functions,thereby providing new insights and experimental evidence for the treatment of various neuromuscular diseases.
基金China Scholarship Council(CSCto XL)and a generous heritage donation from Bettina Fischer,Germany(to JCK).
文摘Aging is characterized by a decreased autophagic activity contributing to the intracellular deposition of damaged organelles and macromolecules.Autophagy is particularly challenging in neurons since autophagic vesicles are formed at the axonal tip and must be transported to the soma where final degradation occurs.Here,we examined if axonal transport of autophagic vesicles is altered during aging.We employed two-photon microscopy for in vivo imaging in the optic nerve of young and aged rats.In old animals(>18 months old),retrograde autophagic vesicle transport was significantly reduced with regard to motility and velocity.While activation of autophagy was decreased,expression of key proteins of the autophagy-lysosomal pathway including p62 and procathepsin D and the number of autophagolysosomes was increased.Maturation of autophagic vesicles was shifted to more distal regions of the axon and axonal lysosomal clearing was impaired.In a pull-down assay,the protein binding between dynein and dynactin was decreased by half,which could explain the retrograde axonal transport effects.Taken together,retrograde axonal autophagic vesicle transport in vivo is diminished during aging accompanied by decreased autophagy activation,alterations of the lysosomal pathway,and a reduced dynein-dynactin binding.
文摘Avulsion injury of one or more spinal ventral roots induces a critical loss of motoneurons,followed by irreversible locomotor function impairment ranging from inadequate limb movement to complete paralysis of the limb.Recent surgical techniques facilitate improvement of limb function,but it remains to be determined exactly how many motoneurons are needed to survive and grow new axons to achieve sufficient muscle reinnervation.The aim of this study was to determine the minimum motoneuron quantity required to reinnervate the denervated skeletal muscles of the limb and produce a functionally satisfactory locomotor pattern.Since none of the commercially available methods and equipment were able to provide a quantifiable and in-depth analysis of the motor pattern of the entire hind limb,we have developed and applied a sensitive movement recording and analyzing system in order to determine the threshold of satisfactory functional reinnervation;we combined video-based footprint analysis and hind limb motion analysis to achieve a new and reliable assessment.Sprague-Dawley rats underwent a lumbar 4-5 ventral root avulsion,and their L4 ventral roots were subsequently reimplanted.The animals received different doses of riluzole treatment in order to rescue incremental numbers of the damaged motoneuron pool.We were able to assess one rear-view and six lateral parameters of the hind limb movement pattern by measuring specific joint angles,footprint,and gait parameters in single video frames.Four months after the operation,we performed Fast Blue retrograde tracing to label and count the reinnervating motoneurons.We then compared the numbers of reinnervating motoneurons and the functional improvement.Our results confirmed a strong relationship between functional restoration of the original movement pattern and morphological reinnervation;approximately 30%of the original motor pool was able to produce a useful locomotor pattern.We believe that our knowledge of the minimal motoneuron numbers required to reinnervate target muscles may help plan the segmental redistribution of the motoneuron pools for reinnervation surgeries.
基金financial supports from National Key R&D Program of China(No.2018YFD0700303)Jiangsu Province Key Laboratory Project of Advanced Food Manufacturing Equipment and Technology(No.FMZ202003)National First-Class Discipline Program of Food Science and Technology(No.JUFSTR20180205).
文摘Hydrophilic colloid-based edible curcumin nanoemulsion coatings was exploited to keep fried rice stabilization in the freeze-thaw cycles(FTCs),including texture,moisture loss,color and other aspects of the quality characteristics.Fried rice were coated with nanoemulsion what was obtained by flaxseed gum(FG),egg white protein(EWP),Olive oil(O),and Curcumin(Cur),namely as CFEO.The scanning electron microscopy(SEM)results showed that the CFEO nanoemulsion coating had a more homogeneous and dense surface structure.In the fried rice quality determination,the hardness of the CFEO nanoemulsion coating was significantly less than the CONTROL group in each cycle.Alternatively,for moisture loss,CONTROL group significantly accelerating the rate of loss.For the color index,the CFEO group had outstanding performance in delaying the increase of the total color difference(ΔE)and brown index(BI),additionally,significantly delayed the decline in whiteness index(WI).Retrogradation enthalpy,crystallinity and short range order analysis demonstrated the contribution of the CFEO group in the delayed starch retrogradation,and further assisted in explaining the changes in texture properties.In addition,the CFEO also played a stabilizing role in preventing the fluctuation of the T_(2) water distribution.Therefore,curcumin nanoemulsion as the coating of fried rice is appropriate and can delay the decline of quality in freeze-thaw cycle.
基金supported by the National Key R&D Program of China(2022YFF1100503,2022YFD2100300/2022YFD2100303)the National Natural Science Foundation of China(No.32001617)+1 种基金the fund of Cultivation Project of Double First-Class Disciplines of Food Science and Engineering,Beijing Technology&Business University(No.19008022213)the fund of Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment&Technology(FM-202306).
文摘In the present study,we investigated the effects of sourdough on the staling rate of bread from the perspectives of starch retrogradation during storage and gluten depolymerization in bread dough.Three sourdough types,which were fermented using Weissella cibaria,Latilactobacillus sakei,and Fructilactobacillus sanfranciscensis strains,were added to bread dough at a 25%ratio.Compared with regular bread,sourdough bread(SDB)exhibited a lower hardness value and slower staling rate during a 7-day storage period.The relative crystallinities and enthalpies revealed that sourdough addition inhibited amylopectin recrystallization.Furthermore,sourdough-induced acid conditions promoted glutenin macropolymer depolymerization;and the dough containing sourdough(SD-D)showed a decrease in viscoelastic properties and changes on microstructure,reflecting a weakened gluten network structure.Among the three sourdough types,L.sakei-fermented sourdough exhibited the lowest staling rate;this might be owing to its higher gluten depolymerization degree,lower α-helix and intermolecular β-sheet contents,and decreased viscoelastic properties.Our study findings provide a theoretical basis for using sourdough fermentation with different strains to develop clean label bread and improve bread quality in the future.
文摘BACKGROUND Endoscopic retrograde cholangiopancreatography(ERCP)is an invasive endoscopic procedure used mainly to treat hepato-pancreato-biliary(HPB)diseases.The need for ERCP has increased with the rising number of HPB diseases over the past decade.Thus,due to increased demand,ERCP is performed at more centers.Currently,it is performed by general surgeons,gastroenterology and invasive radiology specialists in the United States and Europe as recommended by the British Society of Gastroenterology(BSG).AIM To present the results of ERCP procedures from fourteen surgical centers in Türkiye.METHODS Fourteen surgical centers performing ERCP were included in the present study.The age,gender,ERCP indication,success status,post-ERCP complications,ERCP reports and the files of 66993 patients who underwent ERCP were collected from the participating centers.The results are discussed according to the targets declared by the BSG,which are volume load per annum,proportion of successful cannulation(>85%),bile duct clearance rate(>75%),stenting rate for strictures(>80%)and complications(<6%).RESULTS A total of 66993 ERCP procedures were performed in the centers included in the study up to August 2024.29250(43.6%)of the procedures were performed urgently,especially for suppurative cholangitis,biliary tract injuries,etc.The remaining 37743(56.4%)cases were performed electively.50.2%of the patients were female and 49.8%were male.The average ages were 56.5 years for women and 55.9 years for men.General anesthesia was used in 84.1%of the patients while sedation was used in 15.9%.The indications were bile duct stone(78.7%),pancreatic tumor(3.9%),papillary tumor(3.3%),cholangiocarcinoma(2.6%),Oddi sphincter dysfunction(2.4%),bile leakage after cholecystectomy(2%),bile leakage after hydatid cyst surgery(1.9%),biliary stricture(1.7%),and other diseases(3.1%).Hyperamylasemia and post-ERCP pancreatitis were the most common complications as observed in 8.1%of the patients.They were usually self-limited and responded to supportive measures.The frequency of the other complications was also consistent with the literature.CONCLUSION There is a huge shortage of ERCP endoscopists worldwide due to insufficient ERCP training and centers especially in developing and underdeveloped countries.As patients requiring ERCP usually present to surgical practitioners,the incorporation of surgeons into this training program is an effective and reliable solution.The BSG recommends the incorporation of surgeons and radiologists in addition to gastroenterology specialists.This study is the first to present the results of ERCP procedures from fourteen surgical centers throughout Türkiye.The results suggest that the surgical centers included were able to achieve the targets set by the BSG.This study demonstrated that the surgical ERCP units in the present work have reached satisfactory results and provided a reliable and successful ERCP service.There are currently no issues regarding the validity and appropriateness of the surgeons performing ERCP.Therefore,ERCP training should be encouraged in surgeons and more surgical ERCP centers should be provided.
基金supported by grants from the National Nat-ural Science Foundation of China(82222012 and 82120108006)Scientific Innovation Program of Shanghai Municipal Education Committee(201901070007E00052)。
文摘Background:The choice of a basket or a balloon catheter during endoscopic retrograde cholangiopancreatography(ERCP)for the clearance of pancreatic duct stones in patients with chronic pancreatitis(CP)remains controversial.This study compared the efficacy and safety of these two devices for pancreatic duct stone extractions.Methods:We compared the efficacy and safety of basket and balloon catheters for pancreatic stone extractions.We enrolled CP patients who underwent ERCP for the first time at Changhai Hospital,Naval Medical University between February 2012 and December 2021.After propensity score matching(1:1),101 patients were included in each group.The primary outcome was the rate of pancreatic stone clearance.Secondary outcomes included the rate of adverse events during hospitalization,long-term pain relief,and quality of life after one year follow-up period.Results:The rate of complete clearance was comparable between the two groups(86.1%vs.84.2%,P=0.692).In patients with stones≥2 cm before extracorporeal shock wave lithotripsy(ESWL),the rate of complete clearance was significantly higher in the balloon catheter group when compared to the basket catheter group[100%(19/19)vs.70.0%(14/20),P=0.031].In the multivariate logistic analysis,ESWL prior to stone extraction was the only independent predictor of complete clearance[with ESWL 58.4%(264/452)vs.without ESWL 41.6%(188/452),odds ratio=2.3,95%confidence interval:1.2–4.3;P=0.013].No significant differences between groups were found regarding the rates of adverse events during hospitalization,quality of life,and pain relief after one year of follow-up.Conclusions:Basket and balloon catheters showed similar efficacy and safety for pancreatic stone extractions.However,the balloon catheter was superior to the basket catheter if the pancreatic stone size was≥2 cm before ESWL.
文摘Biliary leaks can arise as a consequence of cholecystectomy,liver surgery,liver transplant,or,less frequently,trauma.Early identification and characterization of these leaks are crucial,as they can significantly enhance patient outcomes by reducing morbidity and mortality.Traditionally,surgical repair has been the standard treatment;however,advancements in endoscopic techniques and tools have established endoscopic retrograde cholangiopancreatography(ERCP)as the primary approach for managing these often-complicated cases.Interventions such as sphincterotomy,nasobiliary drainage,and stent placement aim to alleviate the pressure within the bile duct,facilitating depressurization and promoting leak healing.Alongside ERCP,endoscopic ultrasound is playing an increasingly vital role in addressing challenging cases.Ongoing improvements in endoscopic technologies and methodologies offer promising prospects,often minimizing the need for invasive surgical interventions.Nonetheless,the management of biliary leaks continues to pose significant challenges for clinicians.An optimal approach for patients experiencing bile leakage should be determined on a case-by-case basis and discussed within a multidisciplinary team involving radiologists,endoscopists,and surgeons.This comprehensive review aims to elucidate the role of endoscopy in the management of various types of biliary leaks,providing clinicians with practical insights to navigate this complex field.
基金supported by the Special Funding Project for Space Debris and Near-Earth Asteroids Defense Research, China (No. KJSP2023020303)Beijing Municipal Science and Technology Commission, China (No. Z181100002918004)the Youth Innovation Promotion Association, Chinese Academy of Sciences (No. 2022146)
文摘Implementing the flyby to Near-Earth Asteroids (NEAs) with the potential impact risks to the Earth allows for obtaining detailed physical parameters, thereby supporting the high-precision orbit prediction and planetary defense strategy. Different from those conducted asteroid flyby missions, in the 12th China Trajectory Optimization Competition (CTOC-12), a NEAs flyby trajectory design problem using reusable probes that depart from a Lunar Distant Retrograde Orbit (DRO) station in the cislunar space was released. The objective was flyby to as many NEAs as possible using up to 20 probes within a total of 10 years. The ∑ team proposed a solution that can explore 47 NEAs using 11 probes, ranking the first in the competition. In this paper, the methods and results from the winning team are introduced, including mission analysis and preliminary design, and low-energy transfer trajectory optimization. In particular, a round-trip trajectory is divided into three phases: deep space transfer, indirect transfer between the Earth to DRO, and DRO phasing and rendezvous. With the combination of global optimization and local optimization algorithms, the required velocity increments to change the orbital planes are effectively reduced, thus increasing the number of the explored NEAs. The final solution of our team is presented and the results are compared with those of the top three teams. The competition demonstrates that the regularization of flyby missions from the cislunar space to explore NEAs with the potential impact risks to the Earth is the feasible and promising.
文摘BACKGROUND Pancreaticobiliary maljunction(PBM)is a rare congenital abnormality in pancreaticobiliary duct development.PBM is commonly found in children,and it often leads to acute pancreatitis and other diseases as a result of pancreaticobiliary reflux.Roux-en-Y choledochojejunostomy is a common surgical method for the treatment of PBM,but there are several associated complications that may occur after this operation.CASE SUMMARY The patient,a 12-year-old female,was hospitalized nearly 20 times in 2021 for recurrent acute pancreatitis.In 2022,she was diagnosed with PBM and underwent laparoscopic common bile duct resection and Roux-en-Y choledochojejunostomy in a tertiary hospital.In the first year after surgery,the patient had more than 10 recurrent acute pancreatitis episodes.After undergoing abdominal computed tomography and other examinations,she was diagnosed with“residual bile duct stones and recurrent acute pancreatitis”.On January 30,2024,the patient was admitted to our hospital due to recurrent upper abdominal pain and was cured through endoscopic retrograde cholangiopancreatography.CONCLUSION This article reports a case of a child with distal residual common bile duct stones and recurrent acute pancreatitis after Roux-en-Y choledochojejunostomy for PBM.The patient was cured through endoscopic retrograde cholangiopancreatography.
文摘BACKGROUND Common bile duct stones pose a high risk of recurrence or disease progression if not promptly treated.However,there is still no optimal treatment approach.AIM To investigate the clinical efficacy of modified pancreatic duct stent drainage in endoscopic retrograde cholangiopancreatography(ERCP)for treating common bile duct stones.METHODS This retrospective study included 175 patients with common bile duct stones treated at Taizhou Fourth People’s Hospital between January 1,2021,and November 30,2023.The patients were divided into three groups-the modified pancreatic duct stent drainage group(59 cases),the nasobiliary drainage group(58 cases),and the standard biliary drainage group(58 cases).Preoperative general clinical data,laboratory indicators,and the visual analog scale(VAS)at two time points(24 hours before and after surgery)were compared,along with postoperative complications across the three groups.RESULTS Serum levels of aspartate aminotransferase,alanine aminotransferase,alkaline phosphatase,gamma-glutamyltransferase,total bilirubin,direct bilirubin,Creactive protein,and amylase were significantly lower in the modified pancreatic duct stent drainage group and the standard biliary drainage group than those in the nasobiliary drainage group(P<0.05).However,no statistically significant differences were observed in white blood cells,hemoglobin,or neutrophil levels among the three groups(P>0.05).The standard biliary drainage group had significantly lower VAS scores[(4.36±1.18)points]than those for the modified pancreatic duct stent drainage group[(4.92±1.68)points](P=0.033),and the nasobiliary drainage group[(5.54±1.24)points](P=0.017).There were no statistically significant differences in complication rates across the three groups(P>0.05).CONCLUSION Compared to standard biliary drainage and nasobiliary drainage,the modified pancreatic duct stent used during ERCP for patients with bile duct stones significantly reduced hepatocyte injury,improved liver function parameters,alleviated inflammation and pain,enhanced patient comfort,and demonstrated superior safety.
