OBJECTIVE:To evaluate the effects of Rongchang capsule and Xifeng capsule on pentylenetetrazoleinduced epilepsy in zebrafish larvae and to explore the possible mechanisms behind their actions.METHODS:We utilized a tra...OBJECTIVE:To evaluate the effects of Rongchang capsule and Xifeng capsule on pentylenetetrazoleinduced epilepsy in zebrafish larvae and to explore the possible mechanisms behind their actions.METHODS:We utilized a trajectory tracking system to monitor seizures in zebrafish larva to confirm that certain concentrations of Rongchang capsule and Xifeng capsule produce antiepileptic effects.c-fos expression was assessed by quantitative reverse transcription-polymerase chain reaction to validate the efficacy of the capsules.Rest/wake behavior and correlation analysis predicted the targets of Rongchang capsule and Xifeng capsule.RESULTS:Larval movement times and total distances traveled by zebrafish larvae experiencing pentylenetetrazole(PTZ)-induced seizures were decreasedbyvalproatetreatment.Rongchang(500μg/m L)and Xifeng(200μg/m L)rescued the epileptic behaviors and down-regulated c-fos expression in the brains of larvae,which indicated antiepileptic effects.The rest/wake behavioral profiles showed that Rongchang and Xifeng differentially decreased rest time at night and increased larval locomotor activities during the day.Based on correlation between the actions of the two capsules and known compounds,we predicted that they might change rest/wake behaviors by affecting serotonin,GABAergic and histamine signaling pathways.CONCLUSION:The efficacy of Rongchang capsule and Xifeng capsule in alleviating epilepsy-like behaviors and molecular responses was confirmed.Our study provides insight into the capsules'effect on epilepsy.展开更多
Functional near-infrared spectroscopy(fNIRS)was used to explore the effects of sedentary behavior on the brain functional connectivity characteristics of college students in the resting state after recovering from Cor...Functional near-infrared spectroscopy(fNIRS)was used to explore the effects of sedentary behavior on the brain functional connectivity characteristics of college students in the resting state after recovering from Corona Virus Disease 2019(COVID-19).Twenty-two college students with sedentary behavior and 22 college students with sedentary behavior and maintenance of exercise habits were included in the analysis;moreover,8 min fNIRS resting-state data were collected.Based on the concentrations of oxyhemoglobin(HbO2)and deoxyhemoglobin(HbR)in the time series,the resting-state functional connection strength of the two groups of subjects,including the prefrontal cortex(PFC)and the lower limb supplementary motor area(LS),as well as the functional activity and functional connections of the primary motor cortex(M1)were calculated.The following findings were demonstrated.(1)Functional connection analysis based on HbO2 demonstrated that in the comparison of the mean functional connection strength of homologous regions of interest(ROIs)between the sedentary group and the exercise group,there was no significant difference in the mean functional strength of the ROIs between the two groups(p>0:05).In the comparison of the mean functional connection strengths of the two groups of heterologous ROIs,the functional connection strengths of the right PFC and the right LS(p?0:0097),the left LS(p?0:0127),and the right M1(p?0:0305)in the sedentary group were significantly greater.The functional connection strength between the left PFC and the right LS(p?0:0312)and the left LS(p?0:0370)was significantly greater.Additionally,the functional connection strength between the right LS and the right M1(p?0:0370)and the left LS(p?0:0438)was significantly greater.(2)Functional connection analysis based on HbR demonstrated that there was no significant difference in functional connection strength between the sedentary group and the exercise group(p>0:05)or between the sedentary group and the exercise group(p>0:05).Similarly,there was no significant difference in the mean functional connection strength of the homologous and heterologous ROIs of the two groups.Additionally,there was no significant difference in the mean ROIs functional strength between the two groups(p>0:05).Experimental results and graphical analysis based on functional connectivity indicate that in this experiment,college student participants who exhibited sedentary behaviors showed an increase in fNIRS signals.Increase in fNIRS signals among college students exhibiting sedentary behaviors may be linked to their status post-SARS-CoV-2 infection and the sedentary context,potentially contributing to the strengthened functional connectivity in the resting-state cortical brain network.Conversely,the fNIRS signals decreased for the participants with exercise behaviors,who maintained reasonable exercise routines under the same conditions as their sedentary counterparts.The results may suggest that exercise behaviors have the potential to mitigate and reduce the impacts of sedentary behavior on the resting-state cortical brain network.展开更多
目的利用静息态功能磁共振成像探讨重度抑郁障碍(MDD)伴自伤行为患者的脑部自发神经活动与认知情况的关系。方法选取2023年6~12月就诊于南方医科大学南方医院精神心理科且符合DSM-5中MDD诊断标准的成年患者,分为伴自伤组(MDD+NSSI,n=29...目的利用静息态功能磁共振成像探讨重度抑郁障碍(MDD)伴自伤行为患者的脑部自发神经活动与认知情况的关系。方法选取2023年6~12月就诊于南方医科大学南方医院精神心理科且符合DSM-5中MDD诊断标准的成年患者,分为伴自伤组(MDD+NSSI,n=29)和不伴自伤组(MDD-NSSI,n=34),并招募年龄、性别及受教育程度与之匹配健康对照组(n=31)。所有被试完成资料收集、精神评估(HAMD-24、HAMA、C-SSRS和FASM)和认知测试(ANT)。通过对被试原始图像数据进行预处理并提取fALFF值,分析3组间脑功能活动差异有统计学意义的脑区特征,并分析这些脑区信号与临床数据的相关性,探索脑功能异常与临床表现的潜在联系。结果MDD+NSSI组在左额中回、右颞中回处fALFF值增高(P<0.05),在右侧前扣带和旁扣带脑回处降低(P<0.05);MDD-NSSI组在右前楔叶处降低(P<0.05)。MDD+NSSI组左额中回与HAMD呈正相关(r=0.418,P=0.024),MDD-NSSI组右前楔叶与HAMD呈正相关(r=0.357,P=0.038);MDD+NSSI组左额中回与C-SSRS one year呈正相关(r=0.396,P=0.034),右颞中回的与C-SSRS life time呈负相关(r=-0.390,P=0.037),右前扣带和旁扣带脑回与ANT警觉网络效率呈负相关(r=-0.473,P=0.010)。结论MDD+NSSI患者左侧额中回与右侧颞中回的神经活动异常可能反映冲动与情绪处理的变化,前扣带及旁扣带脑回异常具有跨年龄段一致性并影响警觉性;楔前叶异常仅见于MDD NSSI患者,提示两亚型存在不同的情绪和认知处理机制。展开更多
基金Supported by the Chinese National Natural Science Foundation of China:In situ microscopic Analysis and manipulator for Life Science(No.61327802)the Chinese National Natural Science Foundation of China:Functional Nanoparticle Drug Vector for Zebrafish Development and Biocompatible Assessmen(No.81501589)+3 种基金the Chinese National Natural Science Foundation of China:Development of a cross-scale fast AFM system for life science(No.61127006)the Chinese National Natural Science Foundation of China:Study on the Method of Automatic Nano-manipulation for Cell-Oriented Accurate Displacement(No.61633012)the Tianjin Science Technology Research Funds of China:the function of Rab23 gene in zebrafish development(No.14JCQNJC09600)the National Basic Research Program of China:The Basic and Frontier of the New Topological Structure of the Molecular-based Functional Carbon Materials(No.2015CB856500)
文摘OBJECTIVE:To evaluate the effects of Rongchang capsule and Xifeng capsule on pentylenetetrazoleinduced epilepsy in zebrafish larvae and to explore the possible mechanisms behind their actions.METHODS:We utilized a trajectory tracking system to monitor seizures in zebrafish larva to confirm that certain concentrations of Rongchang capsule and Xifeng capsule produce antiepileptic effects.c-fos expression was assessed by quantitative reverse transcription-polymerase chain reaction to validate the efficacy of the capsules.Rest/wake behavior and correlation analysis predicted the targets of Rongchang capsule and Xifeng capsule.RESULTS:Larval movement times and total distances traveled by zebrafish larvae experiencing pentylenetetrazole(PTZ)-induced seizures were decreasedbyvalproatetreatment.Rongchang(500μg/m L)and Xifeng(200μg/m L)rescued the epileptic behaviors and down-regulated c-fos expression in the brains of larvae,which indicated antiepileptic effects.The rest/wake behavioral profiles showed that Rongchang and Xifeng differentially decreased rest time at night and increased larval locomotor activities during the day.Based on correlation between the actions of the two capsules and known compounds,we predicted that they might change rest/wake behaviors by affecting serotonin,GABAergic and histamine signaling pathways.CONCLUSION:The efficacy of Rongchang capsule and Xifeng capsule in alleviating epilepsy-like behaviors and molecular responses was confirmed.Our study provides insight into the capsules'effect on epilepsy.
基金sponsored by the 2022 Heilongjiang Province Education and Teaching Reform Research General Project(Grant Number:SJGY20220644)the Research Funding for Ph.D.Talent Introduction and Research Start-up Fees Project at Harbin Sport University(Grant Number:RC21-202206).
文摘Functional near-infrared spectroscopy(fNIRS)was used to explore the effects of sedentary behavior on the brain functional connectivity characteristics of college students in the resting state after recovering from Corona Virus Disease 2019(COVID-19).Twenty-two college students with sedentary behavior and 22 college students with sedentary behavior and maintenance of exercise habits were included in the analysis;moreover,8 min fNIRS resting-state data were collected.Based on the concentrations of oxyhemoglobin(HbO2)and deoxyhemoglobin(HbR)in the time series,the resting-state functional connection strength of the two groups of subjects,including the prefrontal cortex(PFC)and the lower limb supplementary motor area(LS),as well as the functional activity and functional connections of the primary motor cortex(M1)were calculated.The following findings were demonstrated.(1)Functional connection analysis based on HbO2 demonstrated that in the comparison of the mean functional connection strength of homologous regions of interest(ROIs)between the sedentary group and the exercise group,there was no significant difference in the mean functional strength of the ROIs between the two groups(p>0:05).In the comparison of the mean functional connection strengths of the two groups of heterologous ROIs,the functional connection strengths of the right PFC and the right LS(p?0:0097),the left LS(p?0:0127),and the right M1(p?0:0305)in the sedentary group were significantly greater.The functional connection strength between the left PFC and the right LS(p?0:0312)and the left LS(p?0:0370)was significantly greater.Additionally,the functional connection strength between the right LS and the right M1(p?0:0370)and the left LS(p?0:0438)was significantly greater.(2)Functional connection analysis based on HbR demonstrated that there was no significant difference in functional connection strength between the sedentary group and the exercise group(p>0:05)or between the sedentary group and the exercise group(p>0:05).Similarly,there was no significant difference in the mean functional connection strength of the homologous and heterologous ROIs of the two groups.Additionally,there was no significant difference in the mean ROIs functional strength between the two groups(p>0:05).Experimental results and graphical analysis based on functional connectivity indicate that in this experiment,college student participants who exhibited sedentary behaviors showed an increase in fNIRS signals.Increase in fNIRS signals among college students exhibiting sedentary behaviors may be linked to their status post-SARS-CoV-2 infection and the sedentary context,potentially contributing to the strengthened functional connectivity in the resting-state cortical brain network.Conversely,the fNIRS signals decreased for the participants with exercise behaviors,who maintained reasonable exercise routines under the same conditions as their sedentary counterparts.The results may suggest that exercise behaviors have the potential to mitigate and reduce the impacts of sedentary behavior on the resting-state cortical brain network.
文摘目的利用静息态功能磁共振成像探讨重度抑郁障碍(MDD)伴自伤行为患者的脑部自发神经活动与认知情况的关系。方法选取2023年6~12月就诊于南方医科大学南方医院精神心理科且符合DSM-5中MDD诊断标准的成年患者,分为伴自伤组(MDD+NSSI,n=29)和不伴自伤组(MDD-NSSI,n=34),并招募年龄、性别及受教育程度与之匹配健康对照组(n=31)。所有被试完成资料收集、精神评估(HAMD-24、HAMA、C-SSRS和FASM)和认知测试(ANT)。通过对被试原始图像数据进行预处理并提取fALFF值,分析3组间脑功能活动差异有统计学意义的脑区特征,并分析这些脑区信号与临床数据的相关性,探索脑功能异常与临床表现的潜在联系。结果MDD+NSSI组在左额中回、右颞中回处fALFF值增高(P<0.05),在右侧前扣带和旁扣带脑回处降低(P<0.05);MDD-NSSI组在右前楔叶处降低(P<0.05)。MDD+NSSI组左额中回与HAMD呈正相关(r=0.418,P=0.024),MDD-NSSI组右前楔叶与HAMD呈正相关(r=0.357,P=0.038);MDD+NSSI组左额中回与C-SSRS one year呈正相关(r=0.396,P=0.034),右颞中回的与C-SSRS life time呈负相关(r=-0.390,P=0.037),右前扣带和旁扣带脑回与ANT警觉网络效率呈负相关(r=-0.473,P=0.010)。结论MDD+NSSI患者左侧额中回与右侧颞中回的神经活动异常可能反映冲动与情绪处理的变化,前扣带及旁扣带脑回异常具有跨年龄段一致性并影响警觉性;楔前叶异常仅见于MDD NSSI患者,提示两亚型存在不同的情绪和认知处理机制。
