Objective To investigate the distribution of bra in-derived neurotrophic factor(BDNF) protein in the rabbit retina. Methods Immune response material in the retina was observed using BDNF antibody by the method of i...Objective To investigate the distribution of bra in-derived neurotrophic factor(BDNF) protein in the rabbit retina. Methods Immune response material in the retina was observed using BDNF antibody by the method of immunohistochemistry. Results BDNF gene expression was mainly found in the RGCs, a lso in innernuclei cells and outernuclei cells in rabbit retina. Conclusion RGC is not only the target cell of BDNF, but also express the BDNF protein. BDNF from multi-sources participates in the regulati on of RGCs.展开更多
目的分析老年慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)患者抑郁状况及与临床情况的相关性。方法选择2014年1月-2015年2月354例老年住院COPD患者,通过医院焦虑抑郁情绪测量表(hospital anxiety and depression sca...目的分析老年慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)患者抑郁状况及与临床情况的相关性。方法选择2014年1月-2015年2月354例老年住院COPD患者,通过医院焦虑抑郁情绪测量表(hospital anxiety and depression scale,HAD)进行抑郁状态评估,收集临床资料和肺功能数据并进行相关分析。结果 354例老年住院COPD患者中,抑郁组(HAD≥11分)306例,非抑郁组(HAD<11分)48例。抑郁组和非抑郁组患者年龄、第1秒用力呼气容积(forced expiratory volume in one second,FEV1)、慢性阻塞性肺疾病评估测试(COPD assessment test,CAT)差异无统计学意义(P>0.05);抑郁组患者体质指数(body mass index,BMI)、急性加重次数、FEV1%pred高于非抑郁组(均P<0.05)。相关分析提示CAT和抑郁评分呈正相关(r=0.44,P=0.011);年龄和抑郁评分无相关性(r=0.025,P=0.861);BMI、FEV1%pred和抑郁呈负相关(r=-0.467,P=0.007;r=-0.28,P=0.02)。结论老年住院COPD患者抑郁发病率高,其发生与CAT呈正相关,与BMI、FEV1%pred呈负相关。展开更多
A modification of the mRNA differential display method was used to an alyze differential gene expression during early embryonic development in the mou se. This method detects appropriate changes in the temporal patter...A modification of the mRNA differential display method was used to an alyze differential gene expression during early embryonic development in the mou se. This method detects appropriate changes in the temporal pattern of expressio n of amplicons and proteins by DD PCR and SDS PAGE analysis. In addition, it i ndicates that the activity of genes during early embryonic development was tempo ral; different degrees of activation→expression→translation occurring with cha nging spatio temporal and environmental conditions. The selectivity of gene exp r ession and levels of transcription and translation are a key controlled regulat ed position during early embryonic development in the mouse .展开更多
文摘Objective To investigate the distribution of bra in-derived neurotrophic factor(BDNF) protein in the rabbit retina. Methods Immune response material in the retina was observed using BDNF antibody by the method of immunohistochemistry. Results BDNF gene expression was mainly found in the RGCs, a lso in innernuclei cells and outernuclei cells in rabbit retina. Conclusion RGC is not only the target cell of BDNF, but also express the BDNF protein. BDNF from multi-sources participates in the regulati on of RGCs.
文摘目的分析老年慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)患者抑郁状况及与临床情况的相关性。方法选择2014年1月-2015年2月354例老年住院COPD患者,通过医院焦虑抑郁情绪测量表(hospital anxiety and depression scale,HAD)进行抑郁状态评估,收集临床资料和肺功能数据并进行相关分析。结果 354例老年住院COPD患者中,抑郁组(HAD≥11分)306例,非抑郁组(HAD<11分)48例。抑郁组和非抑郁组患者年龄、第1秒用力呼气容积(forced expiratory volume in one second,FEV1)、慢性阻塞性肺疾病评估测试(COPD assessment test,CAT)差异无统计学意义(P>0.05);抑郁组患者体质指数(body mass index,BMI)、急性加重次数、FEV1%pred高于非抑郁组(均P<0.05)。相关分析提示CAT和抑郁评分呈正相关(r=0.44,P=0.011);年龄和抑郁评分无相关性(r=0.025,P=0.861);BMI、FEV1%pred和抑郁呈负相关(r=-0.467,P=0.007;r=-0.28,P=0.02)。结论老年住院COPD患者抑郁发病率高,其发生与CAT呈正相关,与BMI、FEV1%pred呈负相关。
文摘A modification of the mRNA differential display method was used to an alyze differential gene expression during early embryonic development in the mou se. This method detects appropriate changes in the temporal pattern of expressio n of amplicons and proteins by DD PCR and SDS PAGE analysis. In addition, it i ndicates that the activity of genes during early embryonic development was tempo ral; different degrees of activation→expression→translation occurring with cha nging spatio temporal and environmental conditions. The selectivity of gene exp r ession and levels of transcription and translation are a key controlled regulat ed position during early embryonic development in the mouse .
