Ovarian aging is characterized by a progressive decline in oocyte quality and quantity with age.Icariin(ICA),a flavonoid compound derived from Epimedium species,has demonstrated potential as an agent for ovarian resto...Ovarian aging is characterized by a progressive decline in oocyte quality and quantity with age.Icariin(ICA),a flavonoid compound derived from Epimedium species,has demonstrated potential as an agent for ovarian restoration.In this study,a subcutaneous implantation system using gelatin methacryloyl(GelMA)hydrogel embedded with ICA was developed to restore ovarian function in aged female mice.Mice were assigned to receive subcutaneous implantation of GelMA alone(GelMA group),GelMA containing ICA(GelMA/ICA group),or a sham operation.Ovarian morphology,serum hormone levels,follicle counts across developmental stages,and reproductive outcomes were evaluated.In vitro fertilization(IVF)and embryo culture assays were performed to assess oocyte developmental potential,while a 10 day natural mating trial was conducted to determine fertility restoration.RNA sequencing(RNA-seq)and RT-qPCR were performed to elucidate the underlying molecular mechanisms.Results showed that GelMA/ICA treatment significantly increased ovarian index(0.19±0.01 vs.0.13±0.01,P<0.0001)and follicle numbers at all developmental stages,including primordial(383.33±151.65 vs.107.14±32.26,P<0.0001),primary(203.33±83.22 vs.91.43±27.04,P=0.003),and secondary follicles(154.17±52.00 vs.59.28±20.50,P=0.029)compared to the sham controls.Hormonal analyses revealed a significant reduction in serum follicle-stimulating hormone(FSH,11.97±3.53 vs.53.10±17.89 ng/mL,P=0.0008),accompanied by elevated anti-Müllerian hormone(AMH,22.97±2.26 vs.5.54±1.56 ng/mL,P<0.0001)and estradiol(E2,315.30±37.62 vs.168.5±14.78 pg/mL,P<0.0001).Oocyte yield and developmental potential improved significantly,as reflected by the increased number of superovulated MII oocytes(17.83±5.15 vs.4.83±4.79,P=0.0002),and higher proportions of two-cell(85.90%±6.16%vs.50.00%±10.00%,P=0.0009),four-cell(81.67%±9.76%vs.50.00%±10.00%,P=0.0061),and blastocyst stage embryos(64.25%±10.55%vs.23.33%±15.28%,P=0.0067).Live birth numbers were significantly increased following GelMA/ICA treatment(6.90±3.21 vs.1.72±2.05,P=0.0001).Transcriptomic analysis revealed up-regulation of genes associated with cytoskeletal organization(Vil1,Tubb3),lipid storage(Soat2,Plin4),oocyte maturation(Oosp2),and cytokine secretion(Cxcl12).Collectively,these findings suggest that GelMA/ICA hydrogels effectively reverse key hallmarks of ovarian aging and restore reproductive function in aged mice,offering a promising platform for fertility preservation and a novel therapeutic for future investigations into ovarian aging.展开更多
Accurately forecasting ecosystem services is critical for enhancing our understanding and improving management practices within nature reserves,particularly in light of climate change,land use/cover changes,and their ...Accurately forecasting ecosystem services is critical for enhancing our understanding and improving management practices within nature reserves,particularly in light of climate change,land use/cover changes,and their complex interactions.However,existing studies often fail to fully consider vegetation response,constituting a gap in the comprehensive assessment of changes in ecosystem services.Therefore,a coupled model framework integrating climate change,land use change,and vegetation dynamics was developed to allow for the simulation of dynamic ecosystem service scenarios throughout the twenty-first century.The Jiulianshan National Nature Reserve in Jiangxi Province was considered as the study area.The results showed that ecosystem services and their synergistic effects will be optimized under scenarios that emphasize strict protection of ecological lands and incorporating the SSP1-2.6 scenario.However,sustaining optimized ecosystem services poses significant challenges in scenarios characterized by resource-intensive development and ongoing climate warming,as in the SSP5-8.5 scenario.Notably,discernible variations exist in balancing and synergizing the management of ecosystem services across diverse land uses and forest types.Our study underscores the importance of integrating vegetation response into the framework of ecosystem service forecasting,which is essential for assisting nature reserves in effectively addressing the multifaceted risks associated with climate change and rapid socio-economic development.展开更多
Agricultural land development is a pivotal strategy for addressing the global food security crisis.Barren grassland,especially those in mountainous regions,constitutes critical areas where cultivation can substantiall...Agricultural land development is a pivotal strategy for addressing the global food security crisis.Barren grassland,especially those in mountainous regions,constitutes critical areas where cultivation can substantially enhance land resources.This study highlights the necessity for a precise correlation between land development initiatives and constraints in order to optimize efficiency and enhance the effectiveness of such projects,with the core being the seamless integration of land development engineering and techniques to eliminate agricultural constraints.This study employs a systems engineering approach to classify improvement factors into mobile and fixed categories,elucidating the integration methods of constraint factors.Adhering to the Wooden Barrel Principle,these constraints were rigorously analyzed based on soil quality,land topography,water availability,and agricultural infrastructure.An innovative method of engineering type combination is proposed,which effectively explains the correlation between natural factors combination,project type combination,and target factors combination.It provides a convenient way for the selection of barren grassland development projects and lays a foundation for land planning,development project establishment,program selection,engineering design,and budget preparation.Taking Tang County of China as an example,it is divided into 19 factor improvement areas,a quick reference table of engineering types is established,and 14 main types of engineering combinations are obtained,which lays a foundation for the application of theoretical framework in practice.展开更多
According to low earth orbit（LEO） satellite systems with users of different levels, a dynamic channel reservation scheme based on priorities is proposed. Dynamic calculation of the thresholds for reserved channels i...According to low earth orbit（LEO） satellite systems with users of different levels, a dynamic channel reservation scheme based on priorities is proposed. Dynamic calculation of the thresholds for reserved channels is the key of this strategy. In order to obtain the optimal thresholds, the traffic is predicted based on the high-speed deterministic movement property of LEO satellites firstly. Then, a channel allocation model based on Markov is established. Finally, the solution of the model is obtained based on the genetic algorithm. Without user location, this strategy effectively reduces handover failures and improves channel utilization by adjusting dynamically the thresholds according to traffic conditions. The simulation results show that the system＇s overall quality of service can be improved by this strategy.展开更多
In this paper, a novel signal-to-clipping noise ratio and least squares approximation tone reservation scheme(SCR-LSA TR) is proposed to reduce the peak-to-average power ratio for orthogonal frequency division multipl...In this paper, a novel signal-to-clipping noise ratio and least squares approximation tone reservation scheme(SCR-LSA TR) is proposed to reduce the peak-to-average power ratio for orthogonal frequency division multiplexing systems. During the SCR procedure, only the element with the maximal amplitude is picked for processing, which not only decreases the algorithm complexity, but also helps to overcome the BER deterioration. With the LSA method, the amplitude of the peak-cancelling signals can approximate to that of the original clipping noise as much as possible. Through the combination of the optimization factor in the LSA method, the classic SCR method can achieve better PAPR reduction with faster convergence. Simulation results show that the proposed SCR-LSA TR scheme has less in-band distortion and smaller out-of-band spectral radiation. The BER of the proposed scheme shows a better performance especially under the 16-QAM over the additive white Gaussian noise channel.展开更多
Call admission control (CAC) and resource reservation (RR) for mobile communication are two important factors that guarantee system efficiency and quality of service (QoS) required for different services in a very sca...Call admission control (CAC) and resource reservation (RR) for mobile communication are two important factors that guarantee system efficiency and quality of service (QoS) required for different services in a very scarce resource as the radio spectrum. A new scheme was proposed which extends the concepts of resource sharing and reservations for wideband code division multiple access (WCDMA) systems with a unique feature of soft capacity. Voice and data traffic were considered. The traffic is further classified into handoff and new requests. The reservation thresholds were dynamically adjusted according to the traffic pattern and mobility prediction in order to achieve the maximum channel utilization, while guaranteeing different QoS constraints. The performance of proposed scheme was evaluated using Markov models. New call blocking probability, handoff call dropping probability, and channel utilization were used as benchmarks for the proposed scheme.展开更多
Systemic lupus erythematosus(SLE)is a chronic autoimmune disease in which multiple organs are damaged that prevails in fertile women.Currently,glucocorticoids and immunosuppressants are widely used to treat SLE patien...Systemic lupus erythematosus(SLE)is a chronic autoimmune disease in which multiple organs are damaged that prevails in fertile women.Currently,glucocorticoids and immunosuppressants are widely used to treat SLE patients.However,ovarian dysfunction occurs following the use of these drugs in women with SLE.Here,we summarize recent progress in terms of understanding ovarian injury,the effects of drug application and strategies to improve ovarian function in women with SLE.This review could be helpful to precisely cure SLE in women desiring to have offspring.展开更多
Objective:To conduct a systematic examination and meta-analysis of the most reliable data from experimental studies evaluating the efficacy of autologous platelet-rich plasma(PRP)on low ovarian reserve.Methods:A compr...Objective:To conduct a systematic examination and meta-analysis of the most reliable data from experimental studies evaluating the efficacy of autologous platelet-rich plasma(PRP)on low ovarian reserve.Methods:A comprehensive search was performed utilizing pertinent search terms across electronic databases,including PubMed,Cochrane,and Google Scholar.We included studies that assigned infertile women with low ovarian reserve in experimental studies.Ovarian reserve parameters were measured before and after PRP injection into ovaries.The data of each study was retrieved and subsequently compiled.Results:Of 301 articles collected and reviewed,six studies were finally included in the meta-analysis.Following PRP injection,infertile women showed a non-significant increase in anti-Mullerian hormone(AMH)level(MD=0.10;95%CI-0.04,0.23),a significant increase in antral follicular count(AFC)(MD=1.88;95%CI 0.47,3.29),and a non-significant reduction in follicle-stimulating hormone(FSH)level(MD=-0.22;95%CI-8.32,7.87).Conclusions:Autologous PRP may increase AFC,but not AMH.Although it is found beneficial in enhancing ovarian reserve(AFC),further research with strong evidence is still required.展开更多
Nature reserves play a significant role in providing ecosystem services and are key sites for biodiversity conservation.The Tianchi Bogda Peak Natural Reserve(TBPNR),located in Xinjiang Uygur Autonomous Region,China,i...Nature reserves play a significant role in providing ecosystem services and are key sites for biodiversity conservation.The Tianchi Bogda Peak Natural Reserve(TBPNR),located in Xinjiang Uygur Autonomous Region,China,is an important ecological barrier area in the temperate arid zone.The evaluation of its important ecosystem services is of great significance to improve the management level and ecological protection efficiency of the reserve.In the present study,we assessed the spatiotemporal variations of four ecosystem services(including net primary productivity(NPP),water yield,soil conservation,and habitat quality)in the TBPNR from 2000 to 2020 based on the environmental and social data using the Integrated Valuation of Ecosystem Services and Trade-offs(InVEST)model.In addition,the coldspot and hotspot areas of ecosystem services were identified by hotspot analysis,and the trade-off and synergistic relationships between ecosystem services were analyzed using factor analysis in a geographic detector.During the study period,NPP and soil conservation values in the reserve increased by 48.20%and 25.56%,respectively;conversely,water yield decreased by 16.56%,and there was no significant change in habitat quality.Spatially,both NPP and habitat quality values were higher in the northern part and lower in the southern part,whereas water yield showed an opposite trend.Correlation analysis revealed that NPP showed a synergistic relationship with habitat quality and soil conservation,and exhibited a trade-off relationship with water yield.Water yield and habitat quality also had a trade-off relationship.NPP and habitat quality were affected by annual average temperature and Normalized Difference Vegetation Index(NDVI),respectively,while water yield and soil conservation were more affected by digital elevation model(DEM).Therefore,attention should be paid to the spatial distribution and dynamics of trade-off and synergistic relationships between ecosystem services in future ecological management.The findings of the present study provide a reference that could facilitate the sustainable utilization of ecosystem services in the typical fragile areas of Northwest China.展开更多
Objective: To compare the functional outcomes of patients who had parotid masseter fascia reserved and unreserved flap elevating scheme in parotidectomy, especially the morbidity of Frey's syndrome. Methods: Twent...Objective: To compare the functional outcomes of patients who had parotid masseter fascia reserved and unreserved flap elevating scheme in parotidectomy, especially the morbidity of Frey's syndrome. Methods: Twenty-three patients (group A) had parotid masseter fascia reserved in the parotidectomy, 32 patients (group B) had parotid masseter fascia unreserved in the operation. Compare the morbidity of gustatory sweating syndrome of the two groups. Results: Follow-up after 6 months to 2.5 years, gustatory sweating syndrome occurred in 4 patients of group A (17%), 17 patients in group B (57%). Conclusion: Reservation technic of the parotid masseter fascial in parotidectomy could prevent regeneration of parasympathetic nerve and thus prevent Frey's syndrome post-operation.展开更多
This paper had described the influence of restoration and reservation of Xi'an Qujiang Pond Ruins Park on urban landscape with an emphasis,proposed to promote urban landscape development by making use of cultural ...This paper had described the influence of restoration and reservation of Xi'an Qujiang Pond Ruins Park on urban landscape with an emphasis,proposed to promote urban landscape development by making use of cultural ruins. It analyzed the good and bad effects of Xi'an Qujiang Pond Ruins Park on urban landscape and concluded that cultural ruins should be used to shape new outlook of urban landscape,so as to discuss the momentous significance of cultural ruins in urban landscape.展开更多
[Objective] The aim was to explore the spatial and temporal distribution characteristics of wind energy resource and preservation in central and west area of Hexi Corridor. [Method] By dint of the wind speed data from...[Objective] The aim was to explore the spatial and temporal distribution characteristics of wind energy resource and preservation in central and west area of Hexi Corridor. [Method] By dint of the wind speed data from 1955 to 2007 in ten meteorological observation station in central and west area of Hexi Corridor,and special wind tower fine data from January to December in 2007,the distribution and reserves of the region＇s wind energy resources were studied. [Result] The results showed that environmental wind speed was relatively stable in central and west Hexi Corridor. There were no distinct changes in climate characteristics distribution. There were regional differences in the distribution of wind energy,and there was a large numerical area of wind energy in Gazhou County and Yumen City; Wind energy in the region generally was higher. The wind energy density was above 100 w/m2 in the 10 m layer,around 140 w/m2 in most places,and was more than 200 w/m2 in the large number area. The wind grew in vertical direction along with the linear growth of height. Each 10 m high wind increased to 15 w/m2 averagely,50m layer wind energy was greater than the general 240 w/m2 and there were obvious changes on daily and annual with wind energy in central and west area of Hexi Corridor. The duration from March to May was a wind energy-intensive stage,10m height from the ground in the wind around 10：00 in low-value. After growing from 11：00,it met the day largest number at 18：00,and then reduced gradually. Effective wind speed hours in the region in general were more than 6 200 h,and the value in the large areas was close to 7 600 h. [Conclusion] The study laid basis for the development and application of wind energy in central and west area of Hexi Corridor.展开更多
Dune barrier systems represent highly sought-after coastal landscapes for tourism and urban development around the world.However,a century ago,they were considered hazardous environments due to their great dynamic nat...Dune barrier systems represent highly sought-after coastal landscapes for tourism and urban development around the world.However,a century ago,they were considered hazardous environments due to their great dynamic nature.As a result,stabilization practices were considered necessary.The systematic introduction of fast-growing exotic trees helped stabilize the sand,making it easier for tourism urbanization to take place,but also leading to erosion processes.This paper aims to assess long-term changes in vegetation cover over a large temperate barrier in Argentina.This complex region includes urban resorts,afforestation zones,and protected areas.A GIS-based geospatial analysis was conducted using a large satellite database(>350 images),and the future evolution of the vegetation was modeled.The results revealed two primary spatiotemporal patterns associated with a gradual expansion of vegetation cover,accompanied by a concurrent reduction in sandy areas.In 1986,the dune area comprised 75%more surface than vegetation,whereas in 2021,it represented 60%less than vegetation.Furthermore,the 2050 scenario suggests a potential 40%reduction of dunes in certain areas.It is necessary to enhance management actions aimed at maintaining dune mobility and ensuring local and regional sediment balance.Long-term management strategies must focus on restoring native plant communities and controlling invasive species,and avoiding new dune fixation initiatives based on the introduction of exotic species.展开更多
[Objective] The aim was to explore the effects of cultivation and seed reservation methods on quality and disease/insect damages of maca seed in cold highland areas,providing scientific references for cultivation and ...[Objective] The aim was to explore the effects of cultivation and seed reservation methods on quality and disease/insect damages of maca seed in cold highland areas,providing scientific references for cultivation and seed reservation and planning of maca vareity.[Method] Yellow,purple and black maca were selected and cultivated in the region with an elevation of 3 000 m to be experimental materials and cultivation and seed reservation methods were as follows：maca seed reservation in situ,seed reservation in original soils maca grown after selection of maca balls,and seed reservation in the region with an elevation of 2 700 m after selection of maca ball,seed reservation in greenhouses in the region with an elevation of 2 400 m after selection of maca ball,and growing selected balls in greenhouses and transplanting to the farmlands with an elevation of 2 400 m.[Result] The cultivation and seed reservation methods had significant or extremely significant effects on maca phenological phase;maca in different colors were treated by different cultivation and seed reservation methods,showing insignificant effects on disease and insect damages.[Conclusion] It can be concluded that the selected maca balls stored for 15 d as per the method grown in the region with an elevation of 2 700 m will improve seed quality and reduce disease or insect damages.展开更多
Clarifying the mechanisms through which coal mining affects groundwater storage(GWS)variations is crucial for water resource conservation and sustainable development.The Ordos Mining Region in China,a key energy base ...Clarifying the mechanisms through which coal mining affects groundwater storage(GWS)variations is crucial for water resource conservation and sustainable development.The Ordos Mining Region in China,a key energy base in China with significant strategic importance,has undergone intensive coal mining activities that have substantially disrupted regional groundwater circulation.This study integrated data from the Gravity Recovery and Climate Experiment Satellite(GRACE)and Famine Early Warning Systems Network(FEWS NET)Land Data Assimilation System(FLDAS)models,combined with weighted downscaling methodology and water balance principles,to reconstruct high-resolution(0.01°)terrestrial water storage(TWS)and GWS changes in the Ordos Mining Region,China from April 2002 to December 2021.The accuracy of GWS variations were validated through pumping test measurements.Subsequently,Geodetector analysis was implemented to quantify the contributions of natural and anthropogenic factors to groundwater storage dynamics.Key findings include:1)TWS in the study area showed a fluctuating but overall decreasing trend,with a total reduction of 8901.11 mm during study period.The most significant annual decrease occurred in 2021,reaching 1696.77 mm.2)GWS exhibited an accelerated decline,with an average annual change rate of 44.35 mm/yr,totaling a decrease of 887.05 mm.The lowest annual groundwater storage level was recorded in 2020,reaching 185.69 mm.3)Precipitation(PRE)contributed the most to GWS variation(q=0.52),followed by coal mining water consumption(MWS)(q=0.41).The interaction between PRE and MWS exhibited a nonlinear enhancement effect on GWS changes(0.54).The synergistic effect of natural hydrological factors has a great influence on the change of GWS,but coal mining water consumption will continue to reduce GWS.These findings provide critical references for the management and regulation of groundwater resource in mining regions.展开更多
Objective To evaluate the association of GGN repeat polymorphism of androgen receptor(AR)with ovarian reserve and ovarian response in controlled ovarian stimulation(COS).Methods This genetic association study was cond...Objective To evaluate the association of GGN repeat polymorphism of androgen receptor(AR)with ovarian reserve and ovarian response in controlled ovarian stimulation(COS).Methods This genetic association study was conducted among a total of 361 women aged≤40 years with basal FSH≤12 U/L undergoing the GnRH-agonist long protocol for COS in a university affiliated IVF center.GGN repeat in the AR gene was analyzed with Sanger sequencing.The primary endpoint was the number of antral follicle counts(AFCs),and the secondary endpoints were stimulation days,total dose of gonadotropin(Gn)used,total number of retrieved oocytes,ovarian sensitivity index,and follicular output rate.Results The GGN repeat in exon 1 of the AR gene ranged from 13 to 24,and the median repeat length was 22.Based on the genotypes(S for GGN repeats<22,L for GGN repeats≥22),the patients were divided into 3 groups:SS,SL,and LL.Generalized regression analysis indicated that the number of AFCs in group SS was significantly lower than those in group SL(adjusted β=1.8,95%CI:0.2-3.4,P=0.024)and group LL(adjusted β=1.5,95%CI:0.2-2.7,P=0.021).No significant difference was observed in the number of AFCs between group SL and group LL(P>0.05).Generalized regression analysis indicated no significant differences in ovarian stimulation parameters among the 3 groups,either before or after adjusting for confounding factors(P>0.05).Conclusion GGN repeat length on the AR gene is associated with AFC but not with ovarian response in Chinese women,indicating that AR gene polymorphisms may affect ovarian reserve.展开更多
[Objective] The aim is to conduct systematic diagnoses on the eco-vulnerability in Daqinggou National Natural Reserve. [Method] A kind of ＂problem pile＂ is proposed about 58 interrelated problems of ecological vulne...[Objective] The aim is to conduct systematic diagnoses on the eco-vulnerability in Daqinggou National Natural Reserve. [Method] A kind of ＂problem pile＂ is proposed about 58 interrelated problems of ecological vulnerability by field survey and questionnaires and a ladder diagram of interrelations among the problems is established. The root problems, basic problems, core problems, interrelation and solving approaches are proposed, as well. [Result] It can be concluded from system diagnosis that fragile ecosystem of the Reserve can be observed from poor disasterresistibility, lower species diffusivity, lower system metabolism and poor system restorability. According to system diagnoses, management planning and control subsystem is key for protection of system vulnerability and solving different problems in process-oriented system. Conservation of the Reserve and ecological education on tourists are indispensible. It can be concluded from diagnoses that plant updating, evolution and ill growth in the Reserve are the focus of the vulnerable ecosystem, which influence species diffusivity, system metabolism and restorability and constitute central nodes of process-oriented and effect-oriented subsystems. [Conclusion] The research provides references for solving vulnerability issues of conservation and tourism management in Daqinggou National Nature Reserve.展开更多
The concept of the brain cognitive reserve is derived from the well-acknowledged notion that the degree of brain damage does not always match the severity of clinical symptoms and neurological/cognitive outcomes.It ha...The concept of the brain cognitive reserve is derived from the well-acknowledged notion that the degree of brain damage does not always match the severity of clinical symptoms and neurological/cognitive outcomes.It has been suggested that the size of the brain(brain reserve) and the extent of neural connections acquired through life(neural reserve) set a threshold beyond which noticeable impairments occur.In contrast,cognitive reserve refers to the brain's ability to adapt and reo rganize stru cturally and functionally to resist damage and maintain function,including neural reserve and brain maintenance,resilience,and compensation(Verkhratsky and Zorec,2024).展开更多
CHINA Gaoligongshan National Natural Reserve Rising to over 4,000 metres above sea level in western Yunnan Province,Gaoligong Mountain forms part of the southern Hengduan Mountain Range and is renowned for its rich bi...CHINA Gaoligongshan National Natural Reserve Rising to over 4,000 metres above sea level in western Yunnan Province,Gaoligong Mountain forms part of the southern Hengduan Mountain Range and is renowned for its rich biodiversity and vibrant cultural landscape.Encompassing a vast protected area across the Tengchong and Baoshan regions,the Gaoligongshan National Natural Reserve is a secluded ecological treasure trove.In April 2000,the reserve joined the UNESCO World Network of Biosphere Reserves.展开更多
基金supported by the National Natural Science Foundation of China(82271671)Nanjing Drum Tower Hospital Academic Innovation Peak Fund(2024-DF-02)+4 种基金Clinical Trials from Nanjing Drum Tower Hospital(2023-LCYJ-MS-05)Nanjing International Science and Technology Cooperation Program(202201027)to L.D.Research Project of State Key Laboratory of Reproductive Medicine and Offspring Health(SKLRM-2022D2)Changzhou Medical Center of Nanjing Medical University(CMCM202203)Clinical Trials from Nanjing Drum Tower Hospital(2022-LCYJ-ZD-02)to H.S.
文摘Ovarian aging is characterized by a progressive decline in oocyte quality and quantity with age.Icariin(ICA),a flavonoid compound derived from Epimedium species,has demonstrated potential as an agent for ovarian restoration.In this study,a subcutaneous implantation system using gelatin methacryloyl(GelMA)hydrogel embedded with ICA was developed to restore ovarian function in aged female mice.Mice were assigned to receive subcutaneous implantation of GelMA alone(GelMA group),GelMA containing ICA(GelMA/ICA group),or a sham operation.Ovarian morphology,serum hormone levels,follicle counts across developmental stages,and reproductive outcomes were evaluated.In vitro fertilization(IVF)and embryo culture assays were performed to assess oocyte developmental potential,while a 10 day natural mating trial was conducted to determine fertility restoration.RNA sequencing(RNA-seq)and RT-qPCR were performed to elucidate the underlying molecular mechanisms.Results showed that GelMA/ICA treatment significantly increased ovarian index(0.19±0.01 vs.0.13±0.01,P<0.0001)and follicle numbers at all developmental stages,including primordial(383.33±151.65 vs.107.14±32.26,P<0.0001),primary(203.33±83.22 vs.91.43±27.04,P=0.003),and secondary follicles(154.17±52.00 vs.59.28±20.50,P=0.029)compared to the sham controls.Hormonal analyses revealed a significant reduction in serum follicle-stimulating hormone(FSH,11.97±3.53 vs.53.10±17.89 ng/mL,P=0.0008),accompanied by elevated anti-Müllerian hormone(AMH,22.97±2.26 vs.5.54±1.56 ng/mL,P<0.0001)and estradiol(E2,315.30±37.62 vs.168.5±14.78 pg/mL,P<0.0001).Oocyte yield and developmental potential improved significantly,as reflected by the increased number of superovulated MII oocytes(17.83±5.15 vs.4.83±4.79,P=0.0002),and higher proportions of two-cell(85.90%±6.16%vs.50.00%±10.00%,P=0.0009),four-cell(81.67%±9.76%vs.50.00%±10.00%,P=0.0061),and blastocyst stage embryos(64.25%±10.55%vs.23.33%±15.28%,P=0.0067).Live birth numbers were significantly increased following GelMA/ICA treatment(6.90±3.21 vs.1.72±2.05,P=0.0001).Transcriptomic analysis revealed up-regulation of genes associated with cytoskeletal organization(Vil1,Tubb3),lipid storage(Soat2,Plin4),oocyte maturation(Oosp2),and cytokine secretion(Cxcl12).Collectively,these findings suggest that GelMA/ICA hydrogels effectively reverse key hallmarks of ovarian aging and restore reproductive function in aged mice,offering a promising platform for fertility preservation and a novel therapeutic for future investigations into ovarian aging.
基金supported by the National Natural Science Foundation of China (32160292, 32471681, and 32201575)Jiangxi “Double Thousand Plan”(jxsq2020101080)+1 种基金the Natural Science Foundation of Jiangxi Province (20224BAB205008, 20224BAB213033, and 20242BAB2538)Jiangxi Province Science and Technology+Water Resources” Joint Plan Project (2023KSG01001 and 2022KSG01010)
文摘Accurately forecasting ecosystem services is critical for enhancing our understanding and improving management practices within nature reserves,particularly in light of climate change,land use/cover changes,and their complex interactions.However,existing studies often fail to fully consider vegetation response,constituting a gap in the comprehensive assessment of changes in ecosystem services.Therefore,a coupled model framework integrating climate change,land use change,and vegetation dynamics was developed to allow for the simulation of dynamic ecosystem service scenarios throughout the twenty-first century.The Jiulianshan National Nature Reserve in Jiangxi Province was considered as the study area.The results showed that ecosystem services and their synergistic effects will be optimized under scenarios that emphasize strict protection of ecological lands and incorporating the SSP1-2.6 scenario.However,sustaining optimized ecosystem services poses significant challenges in scenarios characterized by resource-intensive development and ongoing climate warming,as in the SSP5-8.5 scenario.Notably,discernible variations exist in balancing and synergizing the management of ecosystem services across diverse land uses and forest types.Our study underscores the importance of integrating vegetation response into the framework of ecosystem service forecasting,which is essential for assisting nature reserves in effectively addressing the multifaceted risks associated with climate change and rapid socio-economic development.
基金funded by Science and Technology Project of Hebei Education Department[QN2023085].
文摘Agricultural land development is a pivotal strategy for addressing the global food security crisis.Barren grassland,especially those in mountainous regions,constitutes critical areas where cultivation can substantially enhance land resources.This study highlights the necessity for a precise correlation between land development initiatives and constraints in order to optimize efficiency and enhance the effectiveness of such projects,with the core being the seamless integration of land development engineering and techniques to eliminate agricultural constraints.This study employs a systems engineering approach to classify improvement factors into mobile and fixed categories,elucidating the integration methods of constraint factors.Adhering to the Wooden Barrel Principle,these constraints were rigorously analyzed based on soil quality,land topography,water availability,and agricultural infrastructure.An innovative method of engineering type combination is proposed,which effectively explains the correlation between natural factors combination,project type combination,and target factors combination.It provides a convenient way for the selection of barren grassland development projects and lays a foundation for land planning,development project establishment,program selection,engineering design,and budget preparation.Taking Tang County of China as an example,it is divided into 19 factor improvement areas,a quick reference table of engineering types is established,and 14 main types of engineering combinations are obtained,which lays a foundation for the application of theoretical framework in practice.
基金supported by the National Natural Science Foundation of China(7130108161373137)+1 种基金the Natural Science Foundation of Jiangsu Province(BK20130877BK2012833)
文摘According to low earth orbit（LEO） satellite systems with users of different levels, a dynamic channel reservation scheme based on priorities is proposed. Dynamic calculation of the thresholds for reserved channels is the key of this strategy. In order to obtain the optimal thresholds, the traffic is predicted based on the high-speed deterministic movement property of LEO satellites firstly. Then, a channel allocation model based on Markov is established. Finally, the solution of the model is obtained based on the genetic algorithm. Without user location, this strategy effectively reduces handover failures and improves channel utilization by adjusting dynamically the thresholds according to traffic conditions. The simulation results show that the system＇s overall quality of service can be improved by this strategy.
基金support by the National Natural Science Foundation of China (61401360)the Fundamental Research Funds for the Central Universities (3102017zy026)+1 种基金the Natural Science Basic Research Plan in Shaanxi Province of China (2016JM6017)the Scientific Research Program Funded by Shaanxi Provincial Education Department (16JK1702)
文摘In this paper, a novel signal-to-clipping noise ratio and least squares approximation tone reservation scheme(SCR-LSA TR) is proposed to reduce the peak-to-average power ratio for orthogonal frequency division multiplexing systems. During the SCR procedure, only the element with the maximal amplitude is picked for processing, which not only decreases the algorithm complexity, but also helps to overcome the BER deterioration. With the LSA method, the amplitude of the peak-cancelling signals can approximate to that of the original clipping noise as much as possible. Through the combination of the optimization factor in the LSA method, the classic SCR method can achieve better PAPR reduction with faster convergence. Simulation results show that the proposed SCR-LSA TR scheme has less in-band distortion and smaller out-of-band spectral radiation. The BER of the proposed scheme shows a better performance especially under the 16-QAM over the additive white Gaussian noise channel.
文摘Call admission control (CAC) and resource reservation (RR) for mobile communication are two important factors that guarantee system efficiency and quality of service (QoS) required for different services in a very scarce resource as the radio spectrum. A new scheme was proposed which extends the concepts of resource sharing and reservations for wideband code division multiple access (WCDMA) systems with a unique feature of soft capacity. Voice and data traffic were considered. The traffic is further classified into handoff and new requests. The reservation thresholds were dynamically adjusted according to the traffic pattern and mobility prediction in order to achieve the maximum channel utilization, while guaranteeing different QoS constraints. The performance of proposed scheme was evaluated using Markov models. New call blocking probability, handoff call dropping probability, and channel utilization were used as benchmarks for the proposed scheme.
基金Supported by the National Natural Science Foundation of China,No.82271843Key Project supported by Medical Science and Technology Development Foundation,Nanjing Department of Health,No.ZKX20019.
文摘Systemic lupus erythematosus(SLE)is a chronic autoimmune disease in which multiple organs are damaged that prevails in fertile women.Currently,glucocorticoids and immunosuppressants are widely used to treat SLE patients.However,ovarian dysfunction occurs following the use of these drugs in women with SLE.Here,we summarize recent progress in terms of understanding ovarian injury,the effects of drug application and strategies to improve ovarian function in women with SLE.This review could be helpful to precisely cure SLE in women desiring to have offspring.
文摘Objective:To conduct a systematic examination and meta-analysis of the most reliable data from experimental studies evaluating the efficacy of autologous platelet-rich plasma(PRP)on low ovarian reserve.Methods:A comprehensive search was performed utilizing pertinent search terms across electronic databases,including PubMed,Cochrane,and Google Scholar.We included studies that assigned infertile women with low ovarian reserve in experimental studies.Ovarian reserve parameters were measured before and after PRP injection into ovaries.The data of each study was retrieved and subsequently compiled.Results:Of 301 articles collected and reviewed,six studies were finally included in the meta-analysis.Following PRP injection,infertile women showed a non-significant increase in anti-Mullerian hormone(AMH)level(MD=0.10;95%CI-0.04,0.23),a significant increase in antral follicular count(AFC)(MD=1.88;95%CI 0.47,3.29),and a non-significant reduction in follicle-stimulating hormone(FSH)level(MD=-0.22;95%CI-8.32,7.87).Conclusions:Autologous PRP may increase AFC,but not AMH.Although it is found beneficial in enhancing ovarian reserve(AFC),further research with strong evidence is still required.
基金This research was funded by the Key Laboratory for Sustainable Development of Xinjiang's Historical and Cultural Tourism,Xinjiang University,China(LY2022-06)the Tianchi Talent Project.
文摘Nature reserves play a significant role in providing ecosystem services and are key sites for biodiversity conservation.The Tianchi Bogda Peak Natural Reserve(TBPNR),located in Xinjiang Uygur Autonomous Region,China,is an important ecological barrier area in the temperate arid zone.The evaluation of its important ecosystem services is of great significance to improve the management level and ecological protection efficiency of the reserve.In the present study,we assessed the spatiotemporal variations of four ecosystem services(including net primary productivity(NPP),water yield,soil conservation,and habitat quality)in the TBPNR from 2000 to 2020 based on the environmental and social data using the Integrated Valuation of Ecosystem Services and Trade-offs(InVEST)model.In addition,the coldspot and hotspot areas of ecosystem services were identified by hotspot analysis,and the trade-off and synergistic relationships between ecosystem services were analyzed using factor analysis in a geographic detector.During the study period,NPP and soil conservation values in the reserve increased by 48.20%and 25.56%,respectively;conversely,water yield decreased by 16.56%,and there was no significant change in habitat quality.Spatially,both NPP and habitat quality values were higher in the northern part and lower in the southern part,whereas water yield showed an opposite trend.Correlation analysis revealed that NPP showed a synergistic relationship with habitat quality and soil conservation,and exhibited a trade-off relationship with water yield.Water yield and habitat quality also had a trade-off relationship.NPP and habitat quality were affected by annual average temperature and Normalized Difference Vegetation Index(NDVI),respectively,while water yield and soil conservation were more affected by digital elevation model(DEM).Therefore,attention should be paid to the spatial distribution and dynamics of trade-off and synergistic relationships between ecosystem services in future ecological management.The findings of the present study provide a reference that could facilitate the sustainable utilization of ecosystem services in the typical fragile areas of Northwest China.
文摘Objective: To compare the functional outcomes of patients who had parotid masseter fascia reserved and unreserved flap elevating scheme in parotidectomy, especially the morbidity of Frey's syndrome. Methods: Twenty-three patients (group A) had parotid masseter fascia reserved in the parotidectomy, 32 patients (group B) had parotid masseter fascia unreserved in the operation. Compare the morbidity of gustatory sweating syndrome of the two groups. Results: Follow-up after 6 months to 2.5 years, gustatory sweating syndrome occurred in 4 patients of group A (17%), 17 patients in group B (57%). Conclusion: Reservation technic of the parotid masseter fascial in parotidectomy could prevent regeneration of parasympathetic nerve and thus prevent Frey's syndrome post-operation.
文摘This paper had described the influence of restoration and reservation of Xi'an Qujiang Pond Ruins Park on urban landscape with an emphasis,proposed to promote urban landscape development by making use of cultural ruins. It analyzed the good and bad effects of Xi'an Qujiang Pond Ruins Park on urban landscape and concluded that cultural ruins should be used to shape new outlook of urban landscape,so as to discuss the momentous significance of cultural ruins in urban landscape.
基金Supported by National Natural Science Fund (41075008)Chinese Meteorological Climate Changes Program (280200S011000)Gansu Meteorological Bureau Climate Science and Research Program(2011-09)~~
文摘[Objective] The aim was to explore the spatial and temporal distribution characteristics of wind energy resource and preservation in central and west area of Hexi Corridor. [Method] By dint of the wind speed data from 1955 to 2007 in ten meteorological observation station in central and west area of Hexi Corridor,and special wind tower fine data from January to December in 2007,the distribution and reserves of the region＇s wind energy resources were studied. [Result] The results showed that environmental wind speed was relatively stable in central and west Hexi Corridor. There were no distinct changes in climate characteristics distribution. There were regional differences in the distribution of wind energy,and there was a large numerical area of wind energy in Gazhou County and Yumen City; Wind energy in the region generally was higher. The wind energy density was above 100 w/m2 in the 10 m layer,around 140 w/m2 in most places,and was more than 200 w/m2 in the large number area. The wind grew in vertical direction along with the linear growth of height. Each 10 m high wind increased to 15 w/m2 averagely,50m layer wind energy was greater than the general 240 w/m2 and there were obvious changes on daily and annual with wind energy in central and west area of Hexi Corridor. The duration from March to May was a wind energy-intensive stage,10m height from the ground in the wind around 10：00 in low-value. After growing from 11：00,it met the day largest number at 18：00,and then reduced gradually. Effective wind speed hours in the region in general were more than 6 200 h,and the value in the large areas was close to 7 600 h. [Conclusion] The study laid basis for the development and application of wind energy in central and west area of Hexi Corridor.
基金supported by the Ministry of Science,Technology and Innovation of Argentina trough the project“Topografía,escurrimiento superficial y monitoreo de playas en el Partido de Villa Gesell,provincia de Buenos Aires”(Impact.AR N°106-RESOL-2022-224-APN-SACT#MCT)by the Nacional Council of Scientific and Technical Research(CONICET)through the project“Impactos de la reversión del nivel del mar en Buenos Aires,Argentina”(PIP 21/2311220200100041CO-RESOL-2021-1639-APN-DIR#CONICET)。
文摘Dune barrier systems represent highly sought-after coastal landscapes for tourism and urban development around the world.However,a century ago,they were considered hazardous environments due to their great dynamic nature.As a result,stabilization practices were considered necessary.The systematic introduction of fast-growing exotic trees helped stabilize the sand,making it easier for tourism urbanization to take place,but also leading to erosion processes.This paper aims to assess long-term changes in vegetation cover over a large temperate barrier in Argentina.This complex region includes urban resorts,afforestation zones,and protected areas.A GIS-based geospatial analysis was conducted using a large satellite database(>350 images),and the future evolution of the vegetation was modeled.The results revealed two primary spatiotemporal patterns associated with a gradual expansion of vegetation cover,accompanied by a concurrent reduction in sandy areas.In 1986,the dune area comprised 75%more surface than vegetation,whereas in 2021,it represented 60%less than vegetation.Furthermore,the 2050 scenario suggests a potential 40%reduction of dunes in certain areas.It is necessary to enhance management actions aimed at maintaining dune mobility and ensuring local and regional sediment balance.Long-term management strategies must focus on restoring native plant communities and controlling invasive species,and avoiding new dune fixation initiatives based on the introduction of exotic species.
文摘[Objective] The aim was to explore the effects of cultivation and seed reservation methods on quality and disease/insect damages of maca seed in cold highland areas,providing scientific references for cultivation and seed reservation and planning of maca vareity.[Method] Yellow,purple and black maca were selected and cultivated in the region with an elevation of 3 000 m to be experimental materials and cultivation and seed reservation methods were as follows：maca seed reservation in situ,seed reservation in original soils maca grown after selection of maca balls,and seed reservation in the region with an elevation of 2 700 m after selection of maca ball,seed reservation in greenhouses in the region with an elevation of 2 400 m after selection of maca ball,and growing selected balls in greenhouses and transplanting to the farmlands with an elevation of 2 400 m.[Result] The cultivation and seed reservation methods had significant or extremely significant effects on maca phenological phase;maca in different colors were treated by different cultivation and seed reservation methods,showing insignificant effects on disease and insect damages.[Conclusion] It can be concluded that the selected maca balls stored for 15 d as per the method grown in the region with an elevation of 2 700 m will improve seed quality and reduce disease or insect damages.
基金Under the National Key R&D Program Key Project(No.2021YFC3201201)National Natural Science Foundation of China(No.52360032)+2 种基金Basic Scientific Research Business Fee Project of Colleges And Universities Directly Under the Inner Mongolia Autonomous Region(No.JBYYWF2022001)Development Plan of Innovation Team of Colleges And Universities in Inner Mongolia Autonomous Region(No.NMGIRT2313)the Innovation Team of‘Grassland Talents’。
文摘Clarifying the mechanisms through which coal mining affects groundwater storage(GWS)variations is crucial for water resource conservation and sustainable development.The Ordos Mining Region in China,a key energy base in China with significant strategic importance,has undergone intensive coal mining activities that have substantially disrupted regional groundwater circulation.This study integrated data from the Gravity Recovery and Climate Experiment Satellite(GRACE)and Famine Early Warning Systems Network(FEWS NET)Land Data Assimilation System(FLDAS)models,combined with weighted downscaling methodology and water balance principles,to reconstruct high-resolution(0.01°)terrestrial water storage(TWS)and GWS changes in the Ordos Mining Region,China from April 2002 to December 2021.The accuracy of GWS variations were validated through pumping test measurements.Subsequently,Geodetector analysis was implemented to quantify the contributions of natural and anthropogenic factors to groundwater storage dynamics.Key findings include:1)TWS in the study area showed a fluctuating but overall decreasing trend,with a total reduction of 8901.11 mm during study period.The most significant annual decrease occurred in 2021,reaching 1696.77 mm.2)GWS exhibited an accelerated decline,with an average annual change rate of 44.35 mm/yr,totaling a decrease of 887.05 mm.The lowest annual groundwater storage level was recorded in 2020,reaching 185.69 mm.3)Precipitation(PRE)contributed the most to GWS variation(q=0.52),followed by coal mining water consumption(MWS)(q=0.41).The interaction between PRE and MWS exhibited a nonlinear enhancement effect on GWS changes(0.54).The synergistic effect of natural hydrological factors has a great influence on the change of GWS,but coal mining water consumption will continue to reduce GWS.These findings provide critical references for the management and regulation of groundwater resource in mining regions.
文摘Objective To evaluate the association of GGN repeat polymorphism of androgen receptor(AR)with ovarian reserve and ovarian response in controlled ovarian stimulation(COS).Methods This genetic association study was conducted among a total of 361 women aged≤40 years with basal FSH≤12 U/L undergoing the GnRH-agonist long protocol for COS in a university affiliated IVF center.GGN repeat in the AR gene was analyzed with Sanger sequencing.The primary endpoint was the number of antral follicle counts(AFCs),and the secondary endpoints were stimulation days,total dose of gonadotropin(Gn)used,total number of retrieved oocytes,ovarian sensitivity index,and follicular output rate.Results The GGN repeat in exon 1 of the AR gene ranged from 13 to 24,and the median repeat length was 22.Based on the genotypes(S for GGN repeats<22,L for GGN repeats≥22),the patients were divided into 3 groups:SS,SL,and LL.Generalized regression analysis indicated that the number of AFCs in group SS was significantly lower than those in group SL(adjusted β=1.8,95%CI:0.2-3.4,P=0.024)and group LL(adjusted β=1.5,95%CI:0.2-2.7,P=0.021).No significant difference was observed in the number of AFCs between group SL and group LL(P>0.05).Generalized regression analysis indicated no significant differences in ovarian stimulation parameters among the 3 groups,either before or after adjusting for confounding factors(P>0.05).Conclusion GGN repeat length on the AR gene is associated with AFC but not with ovarian response in Chinese women,indicating that AR gene polymorphisms may affect ovarian reserve.
基金Supported by State Forestry Administration"Public Welfare Program"(201204205)~~
文摘[Objective] The aim is to conduct systematic diagnoses on the eco-vulnerability in Daqinggou National Natural Reserve. [Method] A kind of ＂problem pile＂ is proposed about 58 interrelated problems of ecological vulnerability by field survey and questionnaires and a ladder diagram of interrelations among the problems is established. The root problems, basic problems, core problems, interrelation and solving approaches are proposed, as well. [Result] It can be concluded from system diagnosis that fragile ecosystem of the Reserve can be observed from poor disasterresistibility, lower species diffusivity, lower system metabolism and poor system restorability. According to system diagnoses, management planning and control subsystem is key for protection of system vulnerability and solving different problems in process-oriented system. Conservation of the Reserve and ecological education on tourists are indispensible. It can be concluded from diagnoses that plant updating, evolution and ill growth in the Reserve are the focus of the vulnerable ecosystem, which influence species diffusivity, system metabolism and restorability and constitute central nodes of process-oriented and effect-oriented subsystems. [Conclusion] The research provides references for solving vulnerability issues of conservation and tourism management in Daqinggou National Nature Reserve.
文摘The concept of the brain cognitive reserve is derived from the well-acknowledged notion that the degree of brain damage does not always match the severity of clinical symptoms and neurological/cognitive outcomes.It has been suggested that the size of the brain(brain reserve) and the extent of neural connections acquired through life(neural reserve) set a threshold beyond which noticeable impairments occur.In contrast,cognitive reserve refers to the brain's ability to adapt and reo rganize stru cturally and functionally to resist damage and maintain function,including neural reserve and brain maintenance,resilience,and compensation(Verkhratsky and Zorec,2024).
文摘CHINA Gaoligongshan National Natural Reserve Rising to over 4,000 metres above sea level in western Yunnan Province,Gaoligong Mountain forms part of the southern Hengduan Mountain Range and is renowned for its rich biodiversity and vibrant cultural landscape.Encompassing a vast protected area across the Tengchong and Baoshan regions,the Gaoligongshan National Natural Reserve is a secluded ecological treasure trove.In April 2000,the reserve joined the UNESCO World Network of Biosphere Reserves.
