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A novel method for geometric quality assurance of rock joint replicas in direct shear testing-Part 1:Derivation of quality assurance parameters and geometric reproducibility 被引量:1
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作者 J.Larsson F.Johansson +3 位作者 D.Mas Ivars E.Johnson M.Flansbjer N.W.Portal 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2023年第9期2193-2208,共16页
Since each rock joint is unique by nature,the utilization of replicas in direct shear testing is required to carry out experimental parameter studies.However,information about the ability of the replicas to simulate t... Since each rock joint is unique by nature,the utilization of replicas in direct shear testing is required to carry out experimental parameter studies.However,information about the ability of the replicas to simulate the shear mechanical behavior of the rock joint and their dispersion in direct shear testing is lacking.With the aim to facilitate generation of high-quality direct shear test data from replicas,a novel component in the testing procedure is introduced by presenting two parameters for geometric quality assurance.The parameters are derived from surface comparisons of three-dimensional(3D)scanning data of the rock joint and its replicas.The first parameter,smf,captures morphological deviations between the replica and the rock joint surfaces.smf is derived as the standard deviation of the deviations between the coordinate points of the replica and the rock joint.Four sources of errors introduced in the replica manufacturing process employed in this study could be identified.These errors could be minimized,yielding replicas with smf0.06 mm.The second parameter is a vector,VHp100,which describes deviations with respect to the shear direction.It is the projection of the 100 mm long normal vector of the best-fit plane of the replica joint surface to the corresponding plane of the rock joint.VHp100was found to be less than or equal to 0.36 mm in this study.Application of these two geometric quality assurance parameters demonstrates that it is possible to manufacture replicas with high geometric similarity to the rock joint.In a subsequent paper(part 2),smf and VHp100 are incorporated in a novel quality assurance method,in which the parameters shall be evaluated prior to direct shear testing.Replicas having parameter values below established thresholds shall have a known and narrow dispersion and imitate the shear mechanical behavior of the rock joint. 展开更多
关键词 Three-dimensional(3D)scanning Geometric reproducibility Geometric quality assurance replicas Rock joint Surface comparisons
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Novel algorithm for distributed replicas management based on dynamic programming 被引量:1
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作者 Wang Tao Lu Xianliang Hou Mengshu 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2006年第3期669-672,共4页
Replicas can improve the data reliability in distributed system. However, the traditional algorithms for replica management are based on the assumption that all replicas have the uniform reliability, which is inaccura... Replicas can improve the data reliability in distributed system. However, the traditional algorithms for replica management are based on the assumption that all replicas have the uniform reliability, which is inaccurate in some actual systems. To address such problem, a novel algorithm is proposed based on dynamic programming to manage the number and distribution of replicas in different nodes. By using Markov model, replicas management is organized as a multi-phase process, and the recursion equations are provided. In this algorithm, the heterogeneity of nodes, the expense for maintaining replicas and the engaged space have been considered. Under these restricted conditions, this algorithm realizes high data reliability in a distributed system. The results of case analysis prove the feasibility of the algorithm. 展开更多
关键词 DISTRIBUTED replicas MARKOV dynamic programming.
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Application of a recombinant replicase to localize the Trionyx sinensis hemorrhagic syndrome virus and evaluate its effects on antiviral genes of T.sinensis 被引量:1
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作者 Sunjian LYU Xuemei YUAN +5 位作者 Li LIU Haiqi ZHANG Zhe YU Xiaoying HANG Weida SHI Yinglei WU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2021年第4期295-304,共10页
Trionyx sinensis Hemorrhagic Syndrome Virus(TSHSV)is an arterivirus newly discovered in Chinese softshell turtles.Little is known about the effect of antibodies against the virus or the distribution of the virus in di... Trionyx sinensis Hemorrhagic Syndrome Virus(TSHSV)is an arterivirus newly discovered in Chinese softshell turtles.Little is known about the effect of antibodies against the virus or the distribution of the virus in different organs of infected turtles.In this study,a partial protein of TSHSV-HP4 was produced using a prokaryotic expression system,and its polyclonal antibody was generated.The polyclonal antibody was confirmed by western blot and dot enzyme-linked immunosorbent assay(dot-ELISA).The distribution of TSHSV in different organs of T.sinensis was examined by immunohistochemistry(IHC)and the expression of immune-related genes was analyzed using quantitative real-time polymerase chain reaction(qRT-PCR).The results indicated that the recombinant TSHSV-HP4 protein was successfully expressed,and the generated polyclonal antibody showed specific binding to viral particles in the lung tissues of infected turtles.The IHC assay indicated that the virus was highly localized in various cells,including intestinal lymphocytes,enterocytes,kidney epithelial cells,spleen cells,lung macrophages,and cardiomyocytes.The qRT-PCR analysis revealed that TSHSV was detected in all organs tested,including the lungs,liver,kidneys,spleen,and heart.The numbers of viral mRNA copies in lung and heart tissues were significantly higher in the virus-antibody group than in the virus group.The interferonstimulated genes(ISGs),myxovirus resistance protein 2(MX2)and radical S-adenosyl methionine domain containing 2(RSAD2)were highly upregulated in all groups of infected turtles.Antibody-dependent enhancement(ADE)seemed to occur after stimulation by the polyclonal antibody,because significantly greater expression of the two genes was detected in the virus-antibody group than in the virus group.Overall,these results are important in understanding the cell localization of TSHSV and the immune response of infected turtles. 展开更多
关键词 Trionyx sinensis Hemorrhagic Syndrome Virus(TSHSV) replicasE Virus localization Immune genes
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A novel method for geometric quality assurance of rock joint replicas in direct shear testing-Part 2:Validation and mechanical replicability
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作者 J.Larsson F.Johansson +3 位作者 D.Mas Ivars E.Johnson M.Flansbjer N.W.Portal 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2023年第9期2209-2223,共15页
Each rock joint is unique by nature which means that utilization of replicas in direct shear tests is required in experimental parameter studies.However,a method to acquire knowledge about the ability of the replicas ... Each rock joint is unique by nature which means that utilization of replicas in direct shear tests is required in experimental parameter studies.However,a method to acquire knowledge about the ability of the replicas to imitate the shear mechanical behavior of the rock joint and their dispersion in direct shear testing is lacking.In this study,a novel method is presented for geometric quality assurance of replicas.The aim is to facilitate generation of high-quality direct shear testing data as a prerequisite for reliable subsequent analyses of the results.In Part 1 of this study,two quality assurance parameters,smf and V_(Hp100),are derived and their usefulness for evaluation of geometric deviations,i.e.geometric reproducibility,is shown.In Part 2,the parameters are validated by showing a correlation between the parameters and the shear mechanical behavior,which qualifies the parameters for usage in the quality assurance method.Unique results from direct shear tests presenting comparisons between replicas and the rock joint show that replicas fulfilling proposed threshold values of σ_(mf)<0.06 mm and|V_(Hp100)|<0.2 mm have a narrow dispersion and imitate the shear mechanical behavior of the rock joint in all aspects apart from having a slightly lower peak shear strength.The wear in these replicas,which have similar morphology as the rock joint,is in the same areas as in the rock joint.The wear is slightly larger in the rock joint and therefore the discrepancy in peak shear strength derives from differences in material properties,possibly from differences in toughness.It is shown by application of the suggested method that the quality assured replicas manufactured following the process employed in this study phenomenologically capture the shear strength characteristics,which makes them useful in parameter studies. 展开更多
关键词 Three-dimensional(3D)scanning Contact area measurements Direct shear testing Geometric quality assurance Mechanical replicability replicas Rock joint
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Tree Automata for Extracting Consensus from Partial Replicas of a Structured Document
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作者 Maurice Tchoupé Tchendji Milliam M. Zekeng Ndadji 《Journal of Software Engineering and Applications》 2017年第5期432-456,共25页
In an asynchronous cooperative editing workflow of a structured document, each of the co-authors receives in the different phases of the editing process, a copy of the document to insert its contribution. For confiden... In an asynchronous cooperative editing workflow of a structured document, each of the co-authors receives in the different phases of the editing process, a copy of the document to insert its contribution. For confidentiality reasons, this copy may be only a partial replica containing only parts of the (global) document which are of demonstrated interest for the considered co-author. Note that some parts may be a demonstrated interest over a co-author;they will therefore be accessible concurrently. When it’s synchronization time (e.g. at the end of an asynchronous editing phase of the process), we want to merge all contributions of all authors in a single document. Due to the asynchronism of edition and to the potential existence of the document parts offering concurrent access, conflicts may arise and make partial replicas unmergeable in their entirety: they are inconsistent, meaning that they contain conflictual parts. The purpose of this paper is to propose a merging approach said by consensus of such partial replicas using tree automata. Specifically, from the partial replicas updates, we build a tree automaton that accepts exactly the consensus documents. These documents are the maximum prefixes containing no conflict of partial replicas merged. 展开更多
关键词 Structured Documents WORKFLOW of Cooperative Edition MERGING PARTIAL replicas CONFLICT CONSENSUS Tree AUTOMATA AUTOMATA Product Lazy Evaluation
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Carbon Replicas of Porous Concrete Obtained by Chemical Vapor Deposition—Some Aspects of the Synthesis Mechanism
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作者 Olaf Klepel Nina Danneberg +1 位作者 Matthias Suckow Marcel Erlitz 《Materials Sciences and Applications》 2017年第8期614-627,共14页
In this contribution, the template assisted synthesis of porous carbons by chemical vapor deposition in porous concrete templates has been described for the first time. Porous concrete made templates can be obtained i... In this contribution, the template assisted synthesis of porous carbons by chemical vapor deposition in porous concrete templates has been described for the first time. Porous concrete made templates can be obtained in almost any geometrical shape and are therefore attractive templates to prepare porous carbon monoliths. The carbon deposition process in porous concrete follows a three-stage-course consisting in an initial period, a period of fast carbon deposition and a period of slow carbon deposition. The carbon growth within the template pores occurs obviously plug-like from the inner to the outer sphere. Any continuous covering of the template pore walls by carbon could not be observed. In difference to porous concrete, the carbon deposition in silica gel is strongly accompanied by mass transfer limitations. For porous concrete, such strong effect has not been observed obviously due to its hierarchical pore system. The template materials have been loaded with carbon by chemical vapor deposition in a flow reactor. The process of the template pore filling has been characterized by the time dependence of the template mass gain. The materials have been characterized by means of X-ray tomography and nitrogen adsorption at 77 K, respectively. 展开更多
关键词 CARBON REPLICA TEMPLATE POROUS Concrete Chemical Vapor DEPOSITION
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Effects of mutated replicase and movement protein genes on attenuation of tobacco mosaic virus 被引量:1
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作者 杨恭 邱并生 +1 位作者 魏军亚 刘广超 《Science China(Life Sciences)》 SCIE CAS 2001年第6期628-636,共9页
Our previous reports showed that one opal mutation (UGA) and one ochre mutation (UAA) respectively located in the replicase and movement protein (MP) genes of the attenuated tomato mosaic virus K(ToMV-K) contribute to... Our previous reports showed that one opal mutation (UGA) and one ochre mutation (UAA) respectively located in the replicase and movement protein (MP) genes of the attenuated tomato mosaic virus K(ToMV-K) contribute to the viral attenuation. To explore a wider application of this attenuation pattern to other plant viruses, we have constructed three mutants which respectively contain one opal mutation of the replicase gene and/or one ochre mutation of the MP using PCR-mediated site-directed mutagenesis from a virulent tobacco mosaic virus isolated from China (TMV-Cv). Plant infection performed by in vitro transcripts revealed that the MP truncated mutant TMV-Cvmp and the replicase-MP truncated mutant TMV-Cvrase-mp were infectious on both local lesion (Nicotiana tabacum cv. Xanthi NC) and systemic (N. tabacum cv. K326) host plants, while the replicase truncated mutant TMV-Cvrase was non-infectious. The K326 plant infected by TMV-Cvrease-mp displayed on|y a little mild mosaic. By electronic microscopy (EM), plant re-inoculation, RNA Dot-blot, RT-PCR and sequencing we demonstrated that the progeny viruses of TMV-Cvmp and TMV-Cvrease-mp shared similar morphological character with TMV-Cv, owned the abilities to infect, replicate and propagate in the assayed plants, and maintained the mutated sites during infection. These data showed that both the opal and the ochre mutations are able to cooperatively induce the attenuated phenotypes of TMV-Cvrase-mp on plants, indicating that the mutation pattern of ToMV-K could be used to attenuate other virulent plant viruses. 展开更多
关键词 tobacco mosaic virus (TMV) replicasE movement protein (MP) ATTENUATION mutation.
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Achievable Rate Analysis and Optimization of Double-RIS Assisted Spatially Correlated MIMO with Statistical CSI
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作者 Xu Kaizhe Guo Jiajia +2 位作者 Zhang Jun Jin Shi Ma Shaodan 《China Communications》 2025年第1期139-157,共19页
Reconfigurable intelligent surface(RIS)is a novel meta-material which can form a smart radio environment by dynamically altering reflection directions of the impinging electromagnetic waves.In the prior literature,the... Reconfigurable intelligent surface(RIS)is a novel meta-material which can form a smart radio environment by dynamically altering reflection directions of the impinging electromagnetic waves.In the prior literature,the inter-RIS links which also contribute to the performance of the whole system are usually neglected when multiple RISs are deployed.In this paper we investigate a general double-RIS assisted multiple-input multiple-output(MIMO)wireless communication system under spatially correlated non line-of-sight propagation channels,where the cooperation of the double RISs is also considered.The design objective is to maximize the achievable ergodic rate based on full statistical channel state information(CSI).Specifically,we firstly present a closedform asymptotic expression for the achievable ergodic rate by utilizing replica method from statistical physics.Then a full statistical CSI-enabled optimal design is proposed which avoids high pilot training overhead compared to instantaneous CSI-enabled design.To further reduce the signal processing overhead and lower the complexity for practical realization,a common-phase scheme is proposed to design the double RISs.Simulation results show that the derived asymptotic ergodic rate is quite accurate even for small-sized antenna arrays.And the proposed optimization algorithm can achieve substantial gain at the expense of a low overhead and complexity.Furthermore,the cooperative double-RIS assisted MIMO framework is proven to achieve superior ergodic rate performance and high communication reliability under harsh propagation environment. 展开更多
关键词 common reflection pattern double RISs large system analysis MIMO replica method
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Algorithms for online fault tolerance server consolidation
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作者 Boyu Li Bin Wu +6 位作者 Meng Shen Hao Peng Weisheng Li Hong Zhang Jie Gan Zhihong Tian Guangquan Xu 《Digital Communications and Networks》 2025年第2期514-523,共10页
We study a novel replication mechanism to ensure service continuity against multiple simultaneous server failures.In this mechanism,each item represents a computing task and is replicated intoξ+1 servers for some int... We study a novel replication mechanism to ensure service continuity against multiple simultaneous server failures.In this mechanism,each item represents a computing task and is replicated intoξ+1 servers for some integerξ≥1,with workloads specified by the amount of required resources.If one or more servers fail,the affected workloads can be redirected to other servers that host replicas associated with the same item,such that the service is not interrupted by the failure of up toξservers.This requires that any feasible assignment algorithm must reserve some capacity in each server to accommodate the workload redirected from potential failed servers without overloading,and determining the optimal method for reserving capacity becomes a key issue.Unlike existing algorithms that assume that no two servers share replicas of more than one item,we first formulate capacity reservation for a general arbitrary scenario.Due to the combinatorial nature of this problem,finding the optimal solution is difficult.To this end,we propose a Generalized and Simple Calculating Reserved Capacity(GSCRC)algorithm,with a time complexity only related to the number of items packed in the server.In conjunction with GSCRC,we propose a robust replica packing algorithm with capacity optimization(RobustPack),which aims to minimize the number of servers hosting replicas and tolerate multiple server failures.Through theoretical analysis and experimental evaluations,we show that the RobustPack algorithm can achieve better performance. 展开更多
关键词 Cloud computing Server consolidation REPLICA Fault tolerance
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The effect of thermal-hydro-mechanical coupling on grouting in a single fracture under coal mine flowing water conditions
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作者 Dingyang Zhang Dangping Liu 《Deep Underground Science and Engineering》 2025年第2期264-277,共14页
Groundwater inrush is a hazard that always occurs during underground mining.Grouting is one of the most effective processes to seal underground water inflow for hazard prevention.In this study,grouting experiments are... Groundwater inrush is a hazard that always occurs during underground mining.Grouting is one of the most effective processes to seal underground water inflow for hazard prevention.In this study,grouting experiments are conducted by using a visualized transparent single-fracture replica with plane roughness.Image processing and analysis are performed to investigate the thermo–hydro–mechanical coupling effect on the grouting diffusion under coal mine flowing water conditions.The results show that higher ambient temperature leads to shorter initial gel time of chemical grout and leads to a better relative sealing efficiency in the case of a lower flow rate.However,with a higher water flow rate,the relative sealing efficiency is gradually reduced under higher temperature conditions.The grouting pressure,the seepage pressure,and the temperature are measured.The results reveal that the seepage pressure shows a positive correlation with the grouting pressure,while the temperature change shows a negative correlation with the seepage pressure and the grouting pressure.The“equivalent grouting point offset”effect of grouting shows an eccentric elliptical diffusion with larger grouting distance and width under lower temperature conditions. 展开更多
关键词 a single-fracture replica dynamic water flow grout diffusion sealing efficiency water inrush
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Recent advances in fabrication of monolayer colloidal crystals and their inverse replicas 被引量:6
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作者 YE XiaoZhou QI LiMin 《Science China Chemistry》 SCIE EI CAS 2014年第1期58-69,共12页
Monolayer colloidal crystals(MCCs)are two-dimensional(2D)colloidal crystals consisting of a monolayer of monodisperse colloidal particles arrayed with a 2D periodic order.In recent years,MCCs have attracted intensive ... Monolayer colloidal crystals(MCCs)are two-dimensional(2D)colloidal crystals consisting of a monolayer of monodisperse colloidal particles arrayed with a 2D periodic order.In recent years,MCCs have attracted intensive interest because they can act as 2D photonic crystals and be used as versatile templates for fabrication of various 2D nanostructure arrays.In this review,we provide an overview of the recent progress in the controllable fabrication of MCCs and their inverse replicas.First,some newly-developed methods for the self-assembly of MCCs based on different strategies including interfacial assembly and convective assembly are introduced.Second,some representative novel methods regarding the fabrication of various functional2D inverse replicas of MCCs,such as 2D arrays of nanobowls,nanocaps,and hollow spheres,as well as 2D monolayer inverse opals(MIOs),are described.In addition,the potential applications of MCCs and their inverse replicas are discussed. 展开更多
关键词 MONOLAYER COLLOIDAL crystals 2D INVERSE replica nanosphere lithography self-assembly replication fabrication method
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Creating technical heritage object replicas in a virtual environment
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作者 Olga EGOROVA Dmitry SHCHERBININ 《Frontiers of Mechanical Engineering》 SCIE CSCD 2016年第1期108-115,共8页
The paper presents innovative informatics methods for creating virtual technical heritage replicas, which are of significant scientific and practical importance not only to researchers but to the public in general. By... The paper presents innovative informatics methods for creating virtual technical heritage replicas, which are of significant scientific and practical importance not only to researchers but to the public in general. By performing 3D modeling and animation of aircrafts, spaceships, architectural-engineering buildings, and other technical objects, the process of teaming is achieved while promoting the preservation of the replicas for future generations. Modem approaches based on the wide usage of computer technologies attract a greater number of young people to explore the history of science and technology and renew their interest in the field of mechanical engineering. 展开更多
关键词 3D document history of science and technology technical knowledge POPULARIZATION virtual environment REPLICA mechanism and machine science (MMS)
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转PRSV复制酶基因T_2代番木瓜植株的抗病性测定 被引量:27
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作者 阮小蕾 李华平 周国辉 《华南农业大学学报》 CAS CSCD 北大核心 2004年第4期12-15,共4页
转PRSV复制酶基因的番木瓜植株,其自交及杂交二代植株经PCR检测和Southern blot杂交的结果表明:目的基因整合在番木瓜的染色体上.人工接种PRSV的Ys、Vb、Sm株系于分子检测阳性的7~8叶期植株,转基因植株均表现高抗.在定植田间的9个月中... 转PRSV复制酶基因的番木瓜植株,其自交及杂交二代植株经PCR检测和Southern blot杂交的结果表明:目的基因整合在番木瓜的染色体上.人工接种PRSV的Ys、Vb、Sm株系于分子检测阳性的7~8叶期植株,转基因植株均表现高抗.在定植田间的9个月中,转基因植株无一发病,而对照则全部发病. 展开更多
关键词 转PRSV复制酶基因 T2代番木瓜 植株 抗病性测定
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番木瓜环斑病毒复制酶基因的克隆和序列分析 被引量:8
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作者 叶长明 陈谷 +2 位作者 黄俊潮 于湄 李宝健 《中山大学学报(自然科学版)》 CAS CSCD 1996年第6期125-127,共3页
用RT-PCR技术从PRV-AL中分离到复制酶(RP)基因,将基因克隆进载体pUC18,用双脱氧链终止法测定了基因序列,表明其全长为1602bp,与国内外报道的HA5-1、YK和Sm的RP基因相比,同源性分别达82.... 用RT-PCR技术从PRV-AL中分离到复制酶(RP)基因,将基因克隆进载体pUC18,用双脱氧链终止法测定了基因序列,表明其全长为1602bp,与国内外报道的HA5-1、YK和Sm的RP基因相比,同源性分别达82.80%,95.07%和91.83%. 展开更多
关键词 番木瓜 环斑病毒 复制酶 基因 无性系 序列分析
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改造的马铃薯Y病毒复制酶基因介导高度抗病性 被引量:18
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作者 项瑜 杨兰英 +1 位作者 彭学贤 莽克强 《生物工程学报》 CAS CSCD 北大核心 1996年第3期258-265,共8页
提取马铃薯Y病毒中国分离株(PVY-C)的mRNA作为模板,随机六聚脱氧核苷酸和寡聚dT为引物合成了单链cDNA。通过聚合酶链式反应(PCR)获得了PVY-C的核内含体b(NIb)全长cDNA克隆。在对其进行全序列分析的基础上,构建了PVY-C NIb基因全长,5'... 提取马铃薯Y病毒中国分离株(PVY-C)的mRNA作为模板,随机六聚脱氧核苷酸和寡聚dT为引物合成了单链cDNA。通过聚合酶链式反应(PCR)获得了PVY-C的核内含体b(NIb)全长cDNA克隆。在对其进行全序列分析的基础上,构建了PVY-C NIb基因全长,5'端缺失381个碱基和NIb反义RNA三种不同形式高等植物表达载体。在土壤农杆菌LBA4404的介导下,转化烟草生产品种NC89,获得了所有三种表达载体的转基因植株。通过分子生物学检测和抗性分析发现不同形式的NIb基因序列的转基因植株对马铃薯Y病毒表现不同程度的抗性。其中,以5'端缺失的NIb的基因转化植株表现最好,从总共20个这类转化株系中筛选到4个株系至少在100μg/ml PVY-C接种浓度下,表现完全的抗病效果。从总共39个全长NIb基因转化株系中,仅有一个株系,在100μg/ml PVY-C的攻毒接种下具有完全的抗病性。所有33个NIb基因反义RNA的转化植株中,无一株系表现完全的抗病效果,但是有部分株系能不同程度地延缓或减轻发病程度,并有部分植株在发病后50d左右有恢复健康的趋势。虽然能够在上述3种形式的NIb基因序列的转基因植物中检测到相应的RNA的转录产物,但是均未能检测到其相应的蛋白表达产物。 展开更多
关键词 烟草 马铃薯Y病毒 复制酶基因 转基因植物
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一种基于P码的频域直接捕获算法的研究 被引量:18
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作者 田明浩 冯永新 潘成胜 《电子学报》 EI CAS CSCD 北大核心 2007年第3期549-552,共4页
GPS(Global Positioning System)系统采用典型的CDMA(Code Division Mutiple Access)体制,目前扩频PRN码主要是用于标准定位服务的C/A码和用于精确定位服务的P码.P码信号具有很强的抗干扰和保密能力,而P码的捕获通常是利用C/A码来完成的... GPS(Global Positioning System)系统采用典型的CDMA(Code Division Mutiple Access)体制,目前扩频PRN码主要是用于标准定位服务的C/A码和用于精确定位服务的P码.P码信号具有很强的抗干扰和保密能力,而P码的捕获通常是利用C/A码来完成的,因此,实现P码的直接捕获对我国未来发展自己的卫星定位系统具有重要意义.本文从研究信号直接捕获算法角度出发,提出了一种新的P码快速直接捕获算法,并通过仿真实验验证了算法的有效性.仿真结果表明,该算法有效地提高了捕获效率. 展开更多
关键词 GPS(Global POSITIONING Systern) P码 XFAST(Extended REPLICA FOLDING ACQUISITION Search Technique) 均值法 相关
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病毒复制酶基因Nib8和ERF转录因子W17基因枪法共转化小麦 被引量:12
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作者 刘永伟 徐兆师 +4 位作者 杜丽璞 徐惠君 李连城 马有志 陈明 《作物学报》 CAS CSCD 北大核心 2007年第9期1548-1552,共5页
将对小麦黄花叶病毒表现高抗的复制酶基因Nib8和具有广谱抗病性的ERF基因W17分别构建到单子叶高效组成型表达载体上,采用基因枪共转化法转化到小麦品种扬麦12和扬麦16中,PCR检测共获得Nib8基因的阳性转基因植株42株,W17基因的阳性转基... 将对小麦黄花叶病毒表现高抗的复制酶基因Nib8和具有广谱抗病性的ERF基因W17分别构建到单子叶高效组成型表达载体上,采用基因枪共转化法转化到小麦品种扬麦12和扬麦16中,PCR检测共获得Nib8基因的阳性转基因植株42株,W17基因的阳性转基因植株48株,及两个功能基因均为阳性的转基因植株6株。以扬麦12为受体的转化率分别为1.53%(Nib8)、4.87%(W17)和0.42%(Nib8+W17);以扬麦16为受体的转化率分别为2.05%(Nib8)、0.86%(W17)和0.20%(Nib8+W17)。对2个功能基因都呈阳性的6个植株进行Southern blotting分析,进一步证实功能基因已经整合到小麦基因组中。本研究为获得具有综合抗病性的小麦新材料奠定了基础。 展开更多
关键词 小麦 基因枪 ERF转录因子 复制酶基因
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转复制酶基因抗病毒烟草的研究 被引量:9
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作者 朱小平 陈曦 +4 位作者 周翼衡 鲍时来 鲁润龙 王丽莉 尹路明 《中国烟草学报》 EI CAS CSCD 1998年第1期20-23,共4页
将携带烟草花叶病毒(TMV)54KD蛋白基因和黄瓜花叶病毒(CMV)3’端缺失0.9kb的复制酶基因片段的植物表达载体pBICT,通过很痛农杆菌311SE系,利用叶圆盘法转化烟草,在含有卡那霉素的选择培养基上诱导生芽、生根,得再生植株53株。... 将携带烟草花叶病毒(TMV)54KD蛋白基因和黄瓜花叶病毒(CMV)3’端缺失0.9kb的复制酶基因片段的植物表达载体pBICT,通过很痛农杆菌311SE系,利用叶圆盘法转化烟草,在含有卡那霉素的选择培养基上诱导生芽、生根,得再生植株53株。移入大田后,选取5株,提取植物总DNA,Southern杂交检测,结果5株中均有复制酶基因的整合,且转基因植株在大田中表现了良好的抗TMV和CMV能力。 展开更多
关键词 烟草花叶病 黄瓜花叶病 根癌农杆菌 复制酶基因 转基因植株
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烟草抗烟草花叶病毒基因N的研究 被引量:10
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作者 张恒木 陈剑平 程晔 《浙江农业学报》 CSCD 2001年第2期55-60,共6页
烟草的N基因是一个较为理想的抗烟草花叶病毒 )tobaccomosaicvirus,TMV)基因 ,它和TMV之间的互作是植物和病毒互作领域里一个经典的系统。烟草N基因已经分离 ,TMV基因组结构和功能已经研究得很清楚 ,这为进一步研究两者的互作奠定了基... 烟草的N基因是一个较为理想的抗烟草花叶病毒 )tobaccomosaicvirus,TMV)基因 ,它和TMV之间的互作是植物和病毒互作领域里一个经典的系统。烟草N基因已经分离 ,TMV基因组结构和功能已经研究得很清楚 ,这为进一步研究两者的互作奠定了基础。本文介绍了这一领域取得的最新进展和存在的问题。 展开更多
关键词 N基因 烟草 烟草花叶病毒 抗性基因 结构 基因表达 互作 抗病反应 信号传导
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