Each species within the order Thysanoptera has a distinct mitochondrial gene order displaying a high level of gene rearrangement,particularly massive in Phlaeothripidae.To understand the evolutionary patterns of rearr...Each species within the order Thysanoptera has a distinct mitochondrial gene order displaying a high level of gene rearrangement,particularly massive in Phlaeothripidae.To understand the evolutionary patterns of rearrangement in this family,we sequenced the complete mitochondrial genome of Psephenothrips eriobotryae,a notable potential pest of loquat trees.Its mitogenome is a circular DNA molecule(15,413 bp in size)composed of 37 genes:13 PCGs,22 tRNAs,two rRNAs,and two putative control regions(CRs).By comparing with the ancestral mitogenome of arthropods,P.eriobotryae exhibits notable gene rearrangement variations.However,it shares four conserved gene blocks with three closely related species in Phlaeothripinae.In the phylogenetic tree,P.eriobotryae was clustered with these three leaffeeding Phlaeothripinae species.The presence of more similar conserved gene blocks probably indicates a strong correlation between gene rearrangements and the phylogeny within Phlaeothripidae.Furthermore,the mitogenome of P.eriobotryae shows some characteristics:(a)two putative CRs in which CR2 is the copy of partial CR1 with 99.62%sequence similarity,and CR1 consists of two 237 bp repeat unites;(b)high A+T content of 82.6%in overall base composition;(c)trn S1 lacking a complete dihydrouridine(DHU)arm;(d)the protein coding gene,atp8 started with TTG instead of the more common ATN,while nad3 terminated by TAG rather than TAA;(e)translocations and inversions observed in fifteen of the 37 genes.展开更多
The complete mitochondrial genome （mitogenome） of Gampsocleis gratiosa was determined. The 15,929 bp in the size of G. gratiosa mitogenome contains a typical gene content, base composition, and codon usage found in ...The complete mitochondrial genome （mitogenome） of Gampsocleis gratiosa was determined. The 15,929 bp in the size of G. gratiosa mitogenome contains a typical gene content, base composition, and codon usage found in metazoan. All 13 protein coding genes （PCGs） of the G gratiosa mitogenome start with a typical ATN codon. The usual termination codons （TAA and TAG） were found from 10 PCGs. However, the atp6, had4, and had5 had incomplete termination codon （T）. The anticodons of all tRNAs are identical to those observed in Drosophila yakuba and Locusta migratoria, and can be folded in the form of a typical clover leaf structure except for trnS （AGN）. The secondary structure of trnS （AGN） was drawn according with the Steinberg-Cedergren tertiary structure. The A＋T content （67.4%） of the A＋T-rich region is relatively lower among the mitogenome regions, in contrast, it usually contains the highest A＋T content for most insects. Two isolated sequence repeat regions （202 bp） were found in the A＋T-rich region with mapping and secondary structure information.展开更多
基金supported by the Natural Science Basic Research Program of Shaanxi Province(2023-JC-QN-0178)the National Natural Sciences Foundation of China(31702042,32260122)+2 种基金the Youth Innovation Team of Shaanxi University(2023-77)the Science and Technology Planning Project of Inner Mongolia Autonomous Region(2021GG0112)the Higher School Science and Technology Research Major Projects of the Inner Mongolia Autonomous Region(NJZZ22604)。
文摘Each species within the order Thysanoptera has a distinct mitochondrial gene order displaying a high level of gene rearrangement,particularly massive in Phlaeothripidae.To understand the evolutionary patterns of rearrangement in this family,we sequenced the complete mitochondrial genome of Psephenothrips eriobotryae,a notable potential pest of loquat trees.Its mitogenome is a circular DNA molecule(15,413 bp in size)composed of 37 genes:13 PCGs,22 tRNAs,two rRNAs,and two putative control regions(CRs).By comparing with the ancestral mitogenome of arthropods,P.eriobotryae exhibits notable gene rearrangement variations.However,it shares four conserved gene blocks with three closely related species in Phlaeothripinae.In the phylogenetic tree,P.eriobotryae was clustered with these three leaffeeding Phlaeothripinae species.The presence of more similar conserved gene blocks probably indicates a strong correlation between gene rearrangements and the phylogeny within Phlaeothripidae.Furthermore,the mitogenome of P.eriobotryae shows some characteristics:(a)two putative CRs in which CR2 is the copy of partial CR1 with 99.62%sequence similarity,and CR1 consists of two 237 bp repeat unites;(b)high A+T content of 82.6%in overall base composition;(c)trn S1 lacking a complete dihydrouridine(DHU)arm;(d)the protein coding gene,atp8 started with TTG instead of the more common ATN,while nad3 terminated by TAG rather than TAA;(e)translocations and inversions observed in fifteen of the 37 genes.
基金supported by the National Natural Science Foundation of China(No.30470238 and 30670279)Innovation Foundation of the Graduate Student Cultivation of the Shaanxi Normal University(No.2006CXB003).
文摘The complete mitochondrial genome （mitogenome） of Gampsocleis gratiosa was determined. The 15,929 bp in the size of G. gratiosa mitogenome contains a typical gene content, base composition, and codon usage found in metazoan. All 13 protein coding genes （PCGs） of the G gratiosa mitogenome start with a typical ATN codon. The usual termination codons （TAA and TAG） were found from 10 PCGs. However, the atp6, had4, and had5 had incomplete termination codon （T）. The anticodons of all tRNAs are identical to those observed in Drosophila yakuba and Locusta migratoria, and can be folded in the form of a typical clover leaf structure except for trnS （AGN）. The secondary structure of trnS （AGN） was drawn according with the Steinberg-Cedergren tertiary structure. The A＋T content （67.4%） of the A＋T-rich region is relatively lower among the mitogenome regions, in contrast, it usually contains the highest A＋T content for most insects. Two isolated sequence repeat regions （202 bp） were found in the A＋T-rich region with mapping and secondary structure information.
