In order to explicit the environmental activity of heavy metals affected by different organic acids in soil,a batch incubation experiment was explored to investigate the influence of high relative molecular mass organ...In order to explicit the environmental activity of heavy metals affected by different organic acids in soil,a batch incubation experiment was explored to investigate the influence of high relative molecular mass organic acid(HMWOA)(humic acid and fulvic acid)and low relative molecular mass organic acid(LMWOA)(threonic acid and oxalic acid)on the release or immobilization of Pb,Cu and Cd in soils.Results showed that LMWOA,especially threonic acid,had a good performance in the release of Pb,Cu and Cd from soils,and decrease in the fractions of HOAc-extractable,reducible and oxidable Pb,Cu and Cd.Conversely,HMWOA,especially humic acid,decreased the release of Pb,Cu and Cd,while it increased the fractions of HOAc-extractable,reducible and oxidable Pb,Cu and Cd,indicating that HMWOA can immobilize heavy metals.The release of Pb,Cu and Cd caused by LMWOA was attributed to the dissociation of soil organic matter and amorphous iron oxides since the total organic carbon and the water-soluble iron increased.The immobilization of Pb,Cu and Cd by HMWOA was attributed to the adsorption onto HMWOA followed by amorphous iron since HMWOA resulted in a significant decrease of zeta potential and an increase of amorphous iron oxide.It can be concluded that LMWOA has a potential application in soil washing remediation,while HMWOA can be used in the immobilization remediation for heavy metals contaminated soils.展开更多
Fatty acid metabolism is responsible not only for oilseed metabolism but also for plant responses to abiotic stresses. In this study, three novel genes related to fatty acid degradation designated GhACX, Gh4CL, and Gh...Fatty acid metabolism is responsible not only for oilseed metabolism but also for plant responses to abiotic stresses. In this study, three novel genes related to fatty acid degradation designated GhACX, Gh4CL, and GhMFP, respectively, were isolated from Gossypium hirsutum acc. TM-1. The phylogenetic analysis revealed that amino acid sequences of GhACXand GhMFP have the highest homology with those from Vitis vinifera, and Gh4CL has a closer genetic relationship with that from Camellia sinensis. Tissue- and organ-specific analysis showed that the three genes expressed widely in all the tested tissues, including ovules and fiber at different developing stages, with expressed preferentially in some organs. Among them, GhACX showed the most abundant transcripts in seeds at 25 d post anthesis (DPA), however, GhMFP and Gh4CL have the strongest expression level in ovules on the day of anthesis. Based on real-time quantitative RT-PCR, the three genes were differentially regulated when induced under wounding, methyl jasmonate (MeJA), cold, and abscisic acid (ABA) treatments. The characterization and expression pattern of three novel fatty acid degradation related genes will aid both to understand the roles of fatty acid degradation related genes as precursor in stress stimuli and to elucidate the physiological function in cotton oilseed metabolism.展开更多
Objective To investigate the relationship between baseline serum uric acid and the severity of coronary artery disease(CAD)in the first-degree relatives or nonfirst-degree relatives of men with type 2 diabetes.Methods...Objective To investigate the relationship between baseline serum uric acid and the severity of coronary artery disease(CAD)in the first-degree relatives or nonfirst-degree relatives of men with type 2 diabetes.Methods Three hundred and eighty-one men with negative coronary angiography for the first time were divided into diabetes and non-diabetes groups and followed-up for展开更多
In object-oriented database systems(OODBSs),the traditional transaction models are no longer suit- able because of the difference between the object-oriented data model(OODM)and the conventional da- ta models(e.g.rela...In object-oriented database systems(OODBSs),the traditional transaction models are no longer suit- able because of the difference between the object-oriented data model(OODM)and the conventional da- ta models(e.g.relational data model).In this paper,transaction models for advanced databuse applica- tions are reviewed and their shortcomings are analyzed.Exchangeability of operations is proposed in- stead of commuativity and recoverability for using more semantics in transaction management.As a result,an object-oriented transaction model(in short,OOTM)is presented.It is not modeled for some special application,but directly based on object-oriented paradigms.A transaction is regarded as an interpretation of a method.Each transaction(even subtransactions)keeps relative ACID(Atomicity, Consistency,Isolation,Durability )properties,therefore the special problems appearing in OODBSs such as“long transactions”,“visibility of inconsistent database state”can be solved.展开更多
基金the financial supports from the National Natural Science Foundation of China(No.U20A20267)the National Key R&D Program of China(Nos.2020YFC1808002,2021YFC1809203)。
文摘In order to explicit the environmental activity of heavy metals affected by different organic acids in soil,a batch incubation experiment was explored to investigate the influence of high relative molecular mass organic acid(HMWOA)(humic acid and fulvic acid)and low relative molecular mass organic acid(LMWOA)(threonic acid and oxalic acid)on the release or immobilization of Pb,Cu and Cd in soils.Results showed that LMWOA,especially threonic acid,had a good performance in the release of Pb,Cu and Cd from soils,and decrease in the fractions of HOAc-extractable,reducible and oxidable Pb,Cu and Cd.Conversely,HMWOA,especially humic acid,decreased the release of Pb,Cu and Cd,while it increased the fractions of HOAc-extractable,reducible and oxidable Pb,Cu and Cd,indicating that HMWOA can immobilize heavy metals.The release of Pb,Cu and Cd caused by LMWOA was attributed to the dissociation of soil organic matter and amorphous iron oxides since the total organic carbon and the water-soluble iron increased.The immobilization of Pb,Cu and Cd by HMWOA was attributed to the adsorption onto HMWOA followed by amorphous iron since HMWOA resulted in a significant decrease of zeta potential and an increase of amorphous iron oxide.It can be concluded that LMWOA has a potential application in soil washing remediation,while HMWOA can be used in the immobilization remediation for heavy metals contaminated soils.
基金financially supported in part by the National Basic Research Program of China (2011CB109300)the National Transgenic Program, China (2011ZX005-004)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions,China
文摘Fatty acid metabolism is responsible not only for oilseed metabolism but also for plant responses to abiotic stresses. In this study, three novel genes related to fatty acid degradation designated GhACX, Gh4CL, and GhMFP, respectively, were isolated from Gossypium hirsutum acc. TM-1. The phylogenetic analysis revealed that amino acid sequences of GhACXand GhMFP have the highest homology with those from Vitis vinifera, and Gh4CL has a closer genetic relationship with that from Camellia sinensis. Tissue- and organ-specific analysis showed that the three genes expressed widely in all the tested tissues, including ovules and fiber at different developing stages, with expressed preferentially in some organs. Among them, GhACX showed the most abundant transcripts in seeds at 25 d post anthesis (DPA), however, GhMFP and Gh4CL have the strongest expression level in ovules on the day of anthesis. Based on real-time quantitative RT-PCR, the three genes were differentially regulated when induced under wounding, methyl jasmonate (MeJA), cold, and abscisic acid (ABA) treatments. The characterization and expression pattern of three novel fatty acid degradation related genes will aid both to understand the roles of fatty acid degradation related genes as precursor in stress stimuli and to elucidate the physiological function in cotton oilseed metabolism.
文摘Objective To investigate the relationship between baseline serum uric acid and the severity of coronary artery disease(CAD)in the first-degree relatives or nonfirst-degree relatives of men with type 2 diabetes.Methods Three hundred and eighty-one men with negative coronary angiography for the first time were divided into diabetes and non-diabetes groups and followed-up for
文摘In object-oriented database systems(OODBSs),the traditional transaction models are no longer suit- able because of the difference between the object-oriented data model(OODM)and the conventional da- ta models(e.g.relational data model).In this paper,transaction models for advanced databuse applica- tions are reviewed and their shortcomings are analyzed.Exchangeability of operations is proposed in- stead of commuativity and recoverability for using more semantics in transaction management.As a result,an object-oriented transaction model(in short,OOTM)is presented.It is not modeled for some special application,but directly based on object-oriented paradigms.A transaction is regarded as an interpretation of a method.Each transaction(even subtransactions)keeps relative ACID(Atomicity, Consistency,Isolation,Durability )properties,therefore the special problems appearing in OODBSs such as“long transactions”,“visibility of inconsistent database state”can be solved.