Mitogen-activated protein kinases(MAPKs)are a family of proteins that constitute signaling pathways involved in processes that control gene expression,cell division, cell survival,apoptosis,metabolism,differentiation ...Mitogen-activated protein kinases(MAPKs)are a family of proteins that constitute signaling pathways involved in processes that control gene expression,cell division, cell survival,apoptosis,metabolism,differentiation and motility.The MAPK pathways can be divided into conventional and atypical MAPK pathways.The first group converts a signal into a cellular response through a relay of three consecutive phosphorylation events exerted by MAPK kinase kinases,MAPK kinase,and MAPK.Atypical MAPK pathways are not organized into this three-tiered cascade.MAPK that belongs to both conventional and atypical MAPK pathways can phosphorylate both non-protein kinase substrates and other protein kinases.The latter are referred to as MAPK-activated protein kinases.This review focuses on one such MAPK-activated protein kinase,MAPK-activated protein kinase 5(MK5)or p38-regulated/activated protein kinase(PRAK).This protein is highly conserved throughout the animal kingdom and seems to be the target of both conventional and atypical MAPK pathways.Recent findings on the regulation of the activity and subcellular localization,bona fide interaction partners and physiological roles of MK5/PRAK are discussed.展开更多
To elucidate the effect of calcite-regulated activated carbon(AC)structure on low-temperature denitrification performance of SCR catalysts,this work prepared a series of Mn-Ce/De-AC-xCaCO_(3)(x is the calcite content ...To elucidate the effect of calcite-regulated activated carbon(AC)structure on low-temperature denitrification performance of SCR catalysts,this work prepared a series of Mn-Ce/De-AC-xCaCO_(3)(x is the calcite content in coal)catalysts were prepared by the incipient wetness impregnation method,followed by acid washing to remove calcium-containing minerals.Comprehensive characterization and low-temperature denitrification tests revealed that calcite-induced structural modulation of coal-derived AC significantly enhances catalytic activity.Specifically,NO conversion increased from 88.3%of Mn-Ce/De-AC to 91.7%of Mn-Ce/De-AC-1CaCO_(3)(210℃).The improved SCR denitrification activity results from the enhancement of physicochemical properties including higher Mn^(4+)content and Ce^(4+)/Ce^(3+)ratio,an abundance of chemisorbed oxygen and acidic sites,which could strengthen the SCR reaction pathways(richer NH_(3)activated species and bidentate nitrate active species).Therefore,NO removal is enhanced.展开更多
Lithium–sulfur(Li–S)batteries are promisingcandidates for next-generation energy storagegiven their high energy density and potential low cost.Chemically activated carbon(CAC)is often used fortheir cathodes,because ...Lithium–sulfur(Li–S)batteries are promisingcandidates for next-generation energy storagegiven their high energy density and potential low cost.Chemically activated carbon(CAC)is often used fortheir cathodes,because it has a high specific surfacearea for sulfur loading.We have developed a pressurizedphysical activation(PPA)method that producedan activated carbon(PPAC)with a high specific surfacearea comparable to that of CAC.The pore structure of PPAC could be changed and its use as a cathode material for Li–Sbatteries was investigated.Battery tests at different capacity rates(C-rates)showed that it had a much improved high-rate performancewith a discharge capacity of 900 mAh/(g of sulfur)at 1 C,in contrast to only 600 mAh/(g of sulfur)for CAC.Porestructure analyses showed that PPAC prepared at a high activation temperature(1000℃)had unusual channel-like mesoporesbetween the microdomains that are the basic structural units of artificial carbon materials.These are connected to microporesdeveloped in each microdomain,and deliver ions from the surroundings to the internal pores and vice versa.The well-developedmicropores and mesopores of PPAC respectively ensured the high adsorption of lithium polysulfides and a high rate ofion diffusion.Compared to CAC,PPAC is a high-performance,low-cost cathode material that is promising for use in futureLi–S batteries.展开更多
Visible lighting and energy-saving are dual needs of energy efficiency and occupant comfort in modern buildings.In this study,a smart window based on phase-change material VO_(2) is designed and optimized to address t...Visible lighting and energy-saving are dual needs of energy efficiency and occupant comfort in modern buildings.In this study,a smart window based on phase-change material VO_(2) is designed and optimized to address the critical challenges in building energy management.The proposed phase-adaptive radiative(PAR)coating is a multilayer nanostructure consisting of TiO/VO_(2)2/TiO/Ag_(2) and polydimethylsiloxane(PDMS).For different VO_(2) phases,visible transmittance T_(vis)>0.6 and emissivity difference in the atmospheric window Δε_(AW)=0.422 can be achieved,which means the PAR window can transfer interior heat to the outside through thermal radiation for cooling or minimize thermal emission for insulation,while ensuring the transmission of visible light for natural daylighting.Compared to normal glass,the PAR window has an average temperature drop of 14.8℃.The year-round energy-saving calculation for four different cities in China indicates that the PAR window can save 22%-32% of the annual cooling and heating energy consumption by seamlessly transitioning between two phases of VO_(2)modes.The multi-objective optimization of the phase-adaptive radiative smart window provides a potential strategy for energy saving.展开更多
The development of polymeric materials that exhibit blue thermally activated delayed fluorescence(TADF)is of great interest for optoelectronic applications.However,achieving TADF in polymers often requires an elaborat...The development of polymeric materials that exhibit blue thermally activated delayed fluorescence(TADF)is of great interest for optoelectronic applications.However,achieving TADF in polymers often requires an elaborate monomer design.The high-energy local triplet state(^(3)LE)of carbazole complicates its application despite the molecular orbital arrangement being suitable for blue emission.Here,we present an approach to polymer design that makes it possible to solve this problem.We demonstrate the in situ formation of a TADF donor-acceptor system during Suzuki polycondensation,creating an extended carbazole-based donor matrix coupled with a triazine acceptor.The resulting polymer exhibited efficient TADF with a low energy gap(ΔE_(ST))value if a phenyl N-substituent,enabling essential electron delocalization,was present in the carbazole moiety.This work establishes a versatile platform for developing carbazole-based TADF polymers,overcoming the fundamental limitations that hinder their widespread application.展开更多
Soil greenhouse gas(GHG)emissions contribute profoundly to global warming;however,how plant detritus input alters GHG emissions is poorly understood.Here,we used detritus input and removal treatments(i.e.,DIRT:control...Soil greenhouse gas(GHG)emissions contribute profoundly to global warming;however,how plant detritus input alters GHG emissions is poorly understood.Here,we used detritus input and removal treatments(i.e.,DIRT:control,CK;double litter,DL;no roots with double litter,NRDL;no litter,NL;no roots,NR;no roots and no litter,NRNL)to assess the effects of litter and root inputs on soil CO_(2),CH_(4),and N_(2)O fluxes in soils in a coniferous(Pinus yunnanensis)and a broad-leaf forest(Quercus pannosa)in a subalpine region in southwestern China.Litter addition increased CO_(2) emissions on average 22.22%,but did not significantly alter CH_(4) uptake and N_(2)O emission compared to the CK.Litter removal(NL and NRNL)significantly reduced CO_(2) emissions on average 30.22%and N_(2)O emissions on average 31.16%from both forest soils,but did not significantly affect soil CH_(4) uptake.Root removal(NR and NRNL)generally decreased these three soil GHG fluxes.Changes inβ-1,4-glucosidase(BG)involved in C and phospholipid fatty acid(PLFAs)biomass were projected to influence CO_(2) emissions,while soil microclimates(temperature and moisture)combined with BG activity mainly regulated CH_(4) uptake.Alterations in dissolved organic nitrogen,microbial biomass nitrogen and BG were mainly responsible for changes in N_(2)O emissions.Interestingly,coniferous forest soil seemed to promote CH_(4) uptake more than the broad-leaf forest soil,but CO_(2) and N_(2)O fluxes were not significantly affected by the forest types.As expected,litter addition significantly increased the warming potential,while litter removal relatively lowered it.These findings revealed the divergent roles of plant detritus input and forest type in shaping soil GHG fluxes,thereby providing insights into forest management and predicting contributions of subalpine forests to global warming.展开更多
A series of blue and blue‑green Ir(Ⅲ)complexes has been investigated theoretically to explore their electronic structures,photophysical properties,efficiency roll‑off effect,and thermal activation delayed fluorescenc...A series of blue and blue‑green Ir(Ⅲ)complexes has been investigated theoretically to explore their electronic structures,photophysical properties,efficiency roll‑off effect,and thermal activation delayed fluorescence(TADF)properties.All calculations were performed using density functional theory(DFT)and time‑dependent density functional theory(TDDFT).Calculations for electronic structures,frontier molecular orbital characteristics(which determine the efficiency roll‑off effect of the complexes),and photophysical properties were conducted using the Gaussian 09 software package.The calculation of spin‑orbit coupling matrix elements<T|HSOC|S>,which determine the TADF properties of the complexes,was performed using the ORCA software package.The calculation results show that the auxiliary ligand tetraphenylimidodiphosphinate(tpip),a strong electron‑withdrawing group,can mitigate the efficiency roll‑off effect of the complex.Furthermore,TADF is observed in one of the designed complexes,(F_(3)Phppy)_(2)Ir(tpip),where F_(3)Phppy=2‑[4‑(2,4,6‑trifluorophenyl)phenyl]pyridine.展开更多
OBJECTIVE: Exploring the effect of Optimized New Shengmai powder(优化新生脉散方, ONSMP) on myocardial fibrosis in heart failure(HF) based on rat sarcoma(RAS)/rapidly accelerated fibrosarcoma(RAF)/mitogen-activated pro...OBJECTIVE: Exploring the effect of Optimized New Shengmai powder(优化新生脉散方, ONSMP) on myocardial fibrosis in heart failure(HF) based on rat sarcoma(RAS)/rapidly accelerated fibrosarcoma(RAF)/mitogen-activated protein kinase kinase(MEK)/extracellular regulated protein kinases(ERK) signaling pathway. METHODS: Randomized 70 Sprague-Dawley rats into sham(n = 10) and operation(n = 60) groups, then established the HF rat by ligating the left anterior descending branch of the coronary artery. We randomly divided the operation group rats into the model, ONSMP [including low(L), medium(M), and high(H) dose], and enalapril groups. After the 4-week drug intervention, echocardiography examines the cardiac function and calculates the ratios of the whole/left heart to the rat's body weight. Finally, we observed the degree of myocardial fibrosis by pathological sections, determined myocardium collagen(COL) Ⅰ and COL Ⅲ content by enzyme-linked immunosorbent assay, detected the m RNA levels of COL Ⅰ, COL Ⅲ, α-smooth muscle actin(α-SMA), and c-Fos proto-oncogene(c-Fos) by universal real-time, and detected the protein expression of p-RAS, p-RAF, p-MEK1/2, p-ERK1/2, p-ETS-like-1 transcription factor(p-ELK1), p-c-Fos, α-SMA, COL Ⅰ, and COL Ⅲ by Western blot. RESULTS: ONSMP can effectively improve HF rat's cardiac function, decrease cardiac organ coefficient, COL volume fraction, and COL Ⅰ/Ⅲ content, down-regulate the m RNA of COL Ⅰ/Ⅲ, α-SMA and c-Fos, and the protein of p-RAS, p-RAF, p-MEK1/2, p-ERK1/2, p-ELK1, c-Fos, COL Ⅰ/Ⅲ, and α-SMA. CONCLUSIONS: ONSMP can effectively reduce myocardial fibrosis in HF rats, and the mechanism may be related to the inhibition of the RAS/RAF/MEK/ERK signaling pathway.展开更多
Precipitation is often used for the preparation of La(OH)_(3)with precipitants of liquid alkali and ammonia.To solve the problems of high cost and wastewater pollution caused by common precipitants,the active MgO synt...Precipitation is often used for the preparation of La(OH)_(3)with precipitants of liquid alkali and ammonia.To solve the problems of high cost and wastewater pollution caused by common precipitants,the active MgO synthesized by pyrolysis was used as the precipitant to prepare La(OH)_(3).The species distribution of LaCl_(3)and LaCl_(3)-MgCl_(2)mixed system solution was calculated,and the kinetic analysis of the precipi-tation process was carried out to confirm the key factors influencing the precipitation of La(OH)_(3).The results show that La(OH)_(3)with D_(50)of 5.57μm,a specific surface area of 25.70 m^(2)/g,a rod-like shape,and MgO content of 0.044 wt%,was successfully prepared by adding active MgO.The precipitation ratio of La reaches 99.92%.The La(OH)_(3)precipitation is controlled by the diffusion process.The activity of MgO has a significant influence on MgO content in the precipitate.The preparation of La(OH)_(3)by active MgO provides a potential way for an eco-friendly preparation method of rare earth.展开更多
Activated carbons derived from corncob （CACs） were prepared by pyrolysis carbonization and KOH activation. Through modifying activation conditions, samples with large pore volume and ultrahigh BET specific surface a...Activated carbons derived from corncob （CACs） were prepared by pyrolysis carbonization and KOH activation. Through modifying activation conditions, samples with large pore volume and ultrahigh BET specific surface area could be obtained. The sample achieved the highest hydrogen uptake capacity of 5.80 wt% at 40 bar and -196℃ The as-obtained samples were characterized by N2-sorption, transmission electron microscopy （TEM） and scanning electron microscopy （SEM）. Besides, thermogravimetric analysis was also employed to investigate the activation behavior of CACs. Detailed investigation on the activation parameters reveals that moderate activation temperature and heating rate are favorable for preparing CACs with high surface area, large pore volume and optimal pore size distribution. Meanwhile, the micropore volume between 0.65 nm and 0.85 nm along with BET surface area and total pore volume has great effects on hydrogen uptake capacities. The present results indicate that CACs are the most promising materials for hydrogen storage application.展开更多
BACKGROUND Sepsis is a major medical challenge.Magnolol is an active constituent of Houpu that improves tissue function and exerts strong anti-endotoxin and anti-inflammatory effects,but the mechanism by which it redu...BACKGROUND Sepsis is a major medical challenge.Magnolol is an active constituent of Houpu that improves tissue function and exerts strong anti-endotoxin and anti-inflammatory effects,but the mechanism by which it reduces intestinal inflammation in sepsis is yet unclear.AIM To assess the protective effect of magnolol on intestinal mucosal epithelial cells in sepsis and elucidate the underlying mechanisms.METHODS Enzyme-linked immunosorbent assay was used to measure tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),IL-6,and regulated on activation,normal T-cell expressed and secreted(RANTES)levels in serum and ileal tissue in animal studies.The histopathological changes of the ileal mucosa in different groups were observed under a microscope.Cell Counting Kit-8 and cell permeability assays were used to determine the concentration of drug-containing serum that did not affect the activity of Caco2 cells but inhibited lipopolysaccharide(LPS)-induced decrease in permeability.Immunofluorescence and Western blot assays were used to detect the levels of RANTES,inhibitor of nuclear factor kappa-B kinaseβ(IKKβ),phosphorylated IKKβ(p-IKKβ),inhibitor of nuclear factor kappa-B kinaseα(IκBα),p65,and p-p65 proteins in different groups in vitro.RESULTS In rats treated with LPS by intravenous tail injection in the presence or absence of magnolol,magnolol inhibited the expression of proinflammatory cytokines,IL-1β,IL-6,and TNF-αin a dose-dependent manner.In addition,magnolol suppressed the production of RANTES in LPS-stimulated sepsis rats.Moreover,in vitro studies suggested that magnolol inhibited the increase of p65 nucleation,thereby markedly downregulating the production of the phosphorylated form of IKKβin LPS-treated Caco2 cells.Specifically,magnolol inhibited the translocation of the transcription factor nuclear factor-kappa B(NF-κB)from the cytosol into the nucleus and down-regulated the expression level of the chemokine RANTES in LPS-stimulated Caco2 cells.CONCLUSION Magnolol down-regulates RANTES levels by inhibiting the LPS/NF-κB signaling pathways,thereby suppressing IL-1β,IL-6,and TNF-αexpression to alleviate the mucosal barrier dysfunction in sepsis.展开更多
The ultra-low permeability reservoir is regarded as an important energy source for oil and gas resource development and is attracting more and more attention.In this work,the active silica nanofuids were prepared by m...The ultra-low permeability reservoir is regarded as an important energy source for oil and gas resource development and is attracting more and more attention.In this work,the active silica nanofuids were prepared by modifed active silica nanoparticles and surfactant BSSB-12.The dispersion stability tests showed that the hydraulic radius of nanofuids was 58.59 nm and the zeta potential was−48.39 mV.The active nanofuids can simultaneously regulate liquid-liquid interface and solid-liquid interface.The nanofuids can reduce the oil/water interfacial tension(IFT)from 23.5 to 6.7 mN/m,and the oil/water/solid contact angle was altered from 42°to 145°.The spontaneous imbibition tests showed that the oil recovery of 0.1 wt%active nanofuids was 20.5%and 8.5%higher than that of 3 wt%NaCl solution and 0.1 wt%BSSB-12 solution.Finally,the efects of nanofuids on dynamic contact angle,dynamic interfacial tension and moduli were studied from the adsorption behavior of nanofuids at solid-liquid and liquid-liquid interface.The oil detaching and transporting are completed by synergistic efect of wettability alteration and interfacial tension reduction.The fndings of this study can help in better understanding of active nanofuids for EOR in ultra-low permeability reservoirs.展开更多
AIM:To investigate the regulation of mindin expression and the signaling pathway involved during inflammation.METHODS:C57BL/6 mice were treated with 3% dextran sulfate sodium (DSS) in drinking water for 6 d to induce ...AIM:To investigate the regulation of mindin expression and the signaling pathway involved during inflammation.METHODS:C57BL/6 mice were treated with 3% dextran sulfate sodium (DSS) in drinking water for 6 d to induce acute colitis,and then the colon was harvested for histological analysis or for RNA isolation.mRNA expression of mindin and nuclear factor (NF)-κB p65 was analyzed by quantitative real time polymerase chain reaction (RT-PCR) and mindin expression construct was conf irmed by Western blotting.Mouse macrophage and intestinal epithelial lineage cells were stimulated with different cytokines and toll-like receptor (TLR) ligands,before pNF-κB-luciferase activity was assessed using the Dual-Luciferase reporter assay system.RESULTS:mRNA expression of mindin was upregulated 4.7 ± 1.1 fold compared with the baseline during DSS-induced intestinal inflammation in the mice.Stimulation with CpG-ODN (a known TLR-9 ligand) induced 4.2 ± 0.3 fold upregulation of mindin expression in RAW 264.7 cells.Full-length of mindin was cloned from cDNA of mouse mesenteric lymph node,then the pCMV-Mindin-Flag expression vector was established and the protein expression level was confi rmed.Transfection of the mindin construct and stimulation with CpG-ODN signifi cantly increased the NF-κB-luciferase activity by 2.5 ± 0.3 and 4.5 ± 0.5 fold in RAW264.7 and CMT93 cells,respectively (P < 0.01).CONCLUSION:Mindin expression is upregulated during intestinal inflammation and may induce NF-κB promoter activation in a TLR-9 mediated manner.展开更多
Taxation,the primary source of fiscal revenue,has profound implications in guiding resource allocation,promoting economic growth,adjusting social wealth distribution,and enhancing cultural influence.The development of...Taxation,the primary source of fiscal revenue,has profound implications in guiding resource allocation,promoting economic growth,adjusting social wealth distribution,and enhancing cultural influence.The development of e-taxation provides a enhanced security for taxation,but it still faces the risk of inefficiency and tax data leakage.As a decentralized ledger,blockchain provides an effective solution for protecting tax data and avoiding tax-related errors and fraud.The introduction of blockchain into e-taxation protocols can ensure the public verification of taxes.However,balancing taxpayer identity privacy with regulation remains a challenge.In this paper,we propose a blockchain-based anonymous and regulatory e-taxation protocol.This protocol ensures the supervision and tracking of malicious taxpayers while maintaining honest taxpayer identity privacy,reduces the storage needs for public key certificates in the public key infrastructure,and enables selfcertification of taxpayers’public keys and addresses.We formalize the security model of unforgeability for transactions,anonymity for honest taxpayers,and traceability for malicious taxpayers.Security analysis shows that the proposed protocol satisfies unforgeability,anonymity,and traceability.The experimental results of time consumption show that the protocol is feasible in practical applications.展开更多
A plant fermentation was carried out by Lactobaccilli against the Rhizome from Pueraria milifica (f-PMF). This material was evidenced by safe in animal toxic study. The main aim of this study was to revise the traditi...A plant fermentation was carried out by Lactobaccilli against the Rhizome from Pueraria milifica (f-PMF). This material was evidenced by safe in animal toxic study. The main aim of this study was to revise the traditional way of hot water extraction to fermentation so as to use up the original material and finding new activity. We tried to show the new activity through phytoestrogen and immune-competent cells from the host that administrated either of original remedy and the new fermented sample, plus activated water SRE. In mice, compromised host was prepared by cancer chemotherapeutic agent (Mitomycin-C). After administration of f-PMF to immno-suppressed animals, the effects by both samples were augmented by lymphocyte in number and functions, macrophage activities, anti-oxidative activity. However, the intense of effect was much more by fermented one but not by conventional one. The anti-oxidative assay was also carried out ex-vivo system by peritoneal macrophage that we proposed as suitable system for evaluating anti-oxidative assay. In our clinical study by 20 healthy volunteers, granulocyte and lymphocyte ratio was regulated as neutral in peripheral white blood cells, increasing one, two and three weeks after the administration of f-PMF. We have found the significant regulation of blood chemical factors that were important makers for the lifestyle-related diseases. The mechanism of augmentation by probing directory with immuno-electrophoretic method, generating new complement component, especially found by alternative pathway of complement. So we discussed the process concerning designed f-PMF molecule for activation of complement component and bound for the biological activity of each physical component. In a limited condition, fewer numbers of volunteers, the breast size was tending to increase along with the administration time. Including these evidences, we discussed the possibility of this traditional ethnic medicine, originally found and spread in the highland area in Thailand and Myanmar.展开更多
Anaerobic ammonia oxidation(Anammox)is an economical and sustainablewastewater nitrogen removal technology,and its application in the mainstream process is the inevitable trend of the development of Anammox.However,ho...Anaerobic ammonia oxidation(Anammox)is an economical and sustainablewastewater nitrogen removal technology,and its application in the mainstream process is the inevitable trend of the development of Anammox.However,how to effectively enriching Anammox bacteria from the activated sludge remains challenging and restricts its extensive applications.In this study,the rapid and efficient enrichment of Anammox bacteriawas achieved by raising the reflux ratio and nitrogen loading rate(NLR)using conventional activated sludge as the inoculant.In the screening phase(days 1–90),the reflux ratio was increased to discharge partial floc sludge,resulting in the relative abundance of Candidatus Brocadiaceae increased from0.04%to 22.54%,which effectively reduced thematrix and spatial competition between other microorganisms and Anammox bacteria.On day 90,the stoichiometric ratio of the Anammox process closely approached the theoretical value of 1:1.32:0.26,indicating that the Anammox reaction was the primary nitrogen removal process in the system.In the enrichment phase(days 91–238),the NLR increased from 0.43 to 1.20 kgN/(m^(3)·d)and removal efficiency was 71.89%,resulting in the relative abundance of Candidatus Brocadiaceae increased to 61.27%on day 180.The reactor operated steadily from days 444 to 498,maintaining the nitrogen removal rate(NRR)of 3.00 kgN/(m^(3)·d)and achieving successful sludge granulation with the particle size of 392.4μm.In short,this study provided a simple and efficient approach for enriching Anammox bacteria from the activated sludge,supporting to start an Anammox process efficiently.展开更多
Due to the complex geological processes of Qinghai-Tibet Plateau,numerous deposits,especially the large-scale ancient landslide deposits,are characteristic features of the valleys incised in southwestern China.Intense...Due to the complex geological processes of Qinghai-Tibet Plateau,numerous deposits,especially the large-scale ancient landslide deposits,are characteristic features of the valleys incised in southwestern China.Intense water level fluctuations since 2011 in Maoergai Reservoir,China,registered the reactivation of Xierguazi ancient landslide,and presented a significant risk to neighboring facilities.Based on detailed field survey and drilling exploration,the landslide was divided into Zone A and Zone B,and other characterizations of landslide were studied as well.To precisely measure the extent of landslide displacement during filling and drawdown stage,surface displacement monitoring system was deployed on the landslide.The monitoring analyses data reveal that reservoir fluctuation is the dominant factor influencing landslide displacement,especially during drawdown stage.Moreover,a future sliding is anticipated in Zone A,while a creep had already existed in Zone B.A reservoir regulation was then established using the lead-lag correlation between reservoir fluctuation and landslide displacement and landslide stability analysis.In the end,the follow-up deformation monitoring demonstrates that the reservoir regulation controlled the landslide effectively.Landslide control by reservoir regulation in Maoergai can serve as a case study for other settlements involved in similar construction activities.展开更多
Employing multiple metals for synergistic electronic structure regulation emerges as a promising approach to develop highly efficient and robust electrocatalysts for hydrogen evolution at ampere levels.In this study,a...Employing multiple metals for synergistic electronic structure regulation emerges as a promising approach to develop highly efficient and robust electrocatalysts for hydrogen evolution at ampere levels.In this study,a series of Schreibersite-type intermetallic compounds,particularly Mo_(2)Fe_(0.8)Ru_(0.2)P,are synthesized through high-temperature solid-phase synthesis.Experimental results demonstrate that the integration of Ru significantly improves the kinetics of proton adsorption and desorption during the hydrogen evolution reaction(HER).Additionally,density functional theory(DFT)calculations and X-ray absorption near edge structure(XANES)analyses effectively corroborate the pronounced d-orbital hybridization of Fe within the structure,which facilitates the transfer of hydroxide ions and the maintenance of material durability during alkaline HER processes.Remarkably,Mo_(2)Fe_(0.8)Ru_(0.2)P exhibits superior alkaline HER activity,characterized by an overpotential of merely 48 mV at a current density of 10 mA cm^(-2).After prolonged operation of 1000 h at high current densities(1.1 A cm^(-2)),the activity decline remains minimal,under 4%(with overpotential increasing from 258 mV to 268 mV).These results demonstrate the potential of strategically combining metallic elements to design high-performance industrial-grade electrocatalysts.展开更多
Reproductive hormones associated with the hypothalamic-pituitary-gonadal(HPG)axis are closely linked to bone homeostasis.In this study,we demonstrate that Gonadotropin inhibitory hormone(GnIH,one of the key reproducti...Reproductive hormones associated with the hypothalamic-pituitary-gonadal(HPG)axis are closely linked to bone homeostasis.In this study,we demonstrate that Gonadotropin inhibitory hormone(GnIH,one of the key reproductive hormones upstream of the HPG axis)plays an indispensable role in regulating bone homeostasis and maintaining bone mass.We find that deficiency of GnIH or its receptor Gpr147 leads to a significant reduction in bone mineral density(BMD)in mice primarily by enhancement of osteoclast activation in vivo and in vitro.Mechanistically,GnIH/Gpr147 inhibits osteoclastogenesis by the PI3K/AKT,MAPK,NF-κB and Nfatc1 signaling pathways.Furthermore,GnIH treatment was able to alleviate bone loss in aging,ovariectomy(OVX)or LPS-induced mice.Moreover,the therapy using green light promotes the release of GnIH and rescues OVX-induced bone loss.In humans,serum GnIH increases and bone resorption markers decrease after green light exposure.Therefore,our study elucidates that GnIH plays an important role in maintaining bone homeostasis via modulating osteoclast differentiation and demonstrates the potential of GnIH therapy or green light therapy in preventing osteoporosis.展开更多
Swelling-activated Cl- currents, I(ci,swell), were measured during hyposmotic shock in white Leghorn embryonic chick heart cells using the whole-cell recording of patch-clamp technique. Genistein, an inhibitor of prot...Swelling-activated Cl- currents, I(ci,swell), were measured during hyposmotic shock in white Leghorn embryonic chick heart cells using the whole-cell recording of patch-clamp technique. Genistein, an inhibitor of protein tyrosine kinase (PTK), suppressed I(ci,swell), Under isosmotic condition phorbol 12-myristate 13-acetate (PMA), an activator of PKC, elicited the Cl~ current similar to that in hyposmotic solution, whereas hyposmotic shock did not elicit I(ci,swell) in chelerythrine chloride(an inhibitor of PKC)-treated cells. Con-focal microscopy experiments using FITC-phalloidin as a fluorescent label of F-actin showed that the actin network was moved from cortical region of the cell to the center after hyposmotic shock as compared with the image under isosmotic condition. When the cells were treated with cytochalasin B (CB) or cytochalasin D (CD) under isosmotic condition the disruption of the F-actin integrity was observed, and I(ci,Sweii) was not elicited. With combination treatment of CB with PMA, hyposmotic solution could not elicited I(Ci,swell), The results suggested that the role of PTK, probably receptor tyrosine kinase, for regulation of I(ci,sweii) appeared to be at upstream site related to the role of F-actin. Then PKC signal pathway was activated somehow and finally change in the polymerization state of cytoskeleton led to activate the swelling-activated Cl- channels. These results demonstrate clearly that PTK, PKC and F-actin are important factors for regulation of I(Ci,swell) in embryonic chick heart cells as compared with often controversial results reported in different cell types.展开更多
文摘Mitogen-activated protein kinases(MAPKs)are a family of proteins that constitute signaling pathways involved in processes that control gene expression,cell division, cell survival,apoptosis,metabolism,differentiation and motility.The MAPK pathways can be divided into conventional and atypical MAPK pathways.The first group converts a signal into a cellular response through a relay of three consecutive phosphorylation events exerted by MAPK kinase kinases,MAPK kinase,and MAPK.Atypical MAPK pathways are not organized into this three-tiered cascade.MAPK that belongs to both conventional and atypical MAPK pathways can phosphorylate both non-protein kinase substrates and other protein kinases.The latter are referred to as MAPK-activated protein kinases.This review focuses on one such MAPK-activated protein kinase,MAPK-activated protein kinase 5(MK5)or p38-regulated/activated protein kinase(PRAK).This protein is highly conserved throughout the animal kingdom and seems to be the target of both conventional and atypical MAPK pathways.Recent findings on the regulation of the activity and subcellular localization,bona fide interaction partners and physiological roles of MK5/PRAK are discussed.
基金Supported by the Science and Technology Cooperation and Exchange special project of Cooperation of Shanxi Province(202404041101014)the Fundamental Research Program of Shanxi Province(202403021212333)+3 种基金the Joint Funds of the National Natural Science Foundation of China(U24A20555)the Lvliang Key R&D of University-Local Cooperation(2023XDHZ10)the Initiation Fund for Doctoral Research of Taiyuan University of Science and Technology(20242026)the Outstanding Doctor Funding Award of Shanxi Province(20242080).
文摘To elucidate the effect of calcite-regulated activated carbon(AC)structure on low-temperature denitrification performance of SCR catalysts,this work prepared a series of Mn-Ce/De-AC-xCaCO_(3)(x is the calcite content in coal)catalysts were prepared by the incipient wetness impregnation method,followed by acid washing to remove calcium-containing minerals.Comprehensive characterization and low-temperature denitrification tests revealed that calcite-induced structural modulation of coal-derived AC significantly enhances catalytic activity.Specifically,NO conversion increased from 88.3%of Mn-Ce/De-AC to 91.7%of Mn-Ce/De-AC-1CaCO_(3)(210℃).The improved SCR denitrification activity results from the enhancement of physicochemical properties including higher Mn^(4+)content and Ce^(4+)/Ce^(3+)ratio,an abundance of chemisorbed oxygen and acidic sites,which could strengthen the SCR reaction pathways(richer NH_(3)activated species and bidentate nitrate active species).Therefore,NO removal is enhanced.
文摘Lithium–sulfur(Li–S)batteries are promisingcandidates for next-generation energy storagegiven their high energy density and potential low cost.Chemically activated carbon(CAC)is often used fortheir cathodes,because it has a high specific surfacearea for sulfur loading.We have developed a pressurizedphysical activation(PPA)method that producedan activated carbon(PPAC)with a high specific surfacearea comparable to that of CAC.The pore structure of PPAC could be changed and its use as a cathode material for Li–Sbatteries was investigated.Battery tests at different capacity rates(C-rates)showed that it had a much improved high-rate performancewith a discharge capacity of 900 mAh/(g of sulfur)at 1 C,in contrast to only 600 mAh/(g of sulfur)for CAC.Porestructure analyses showed that PPAC prepared at a high activation temperature(1000℃)had unusual channel-like mesoporesbetween the microdomains that are the basic structural units of artificial carbon materials.These are connected to microporesdeveloped in each microdomain,and deliver ions from the surroundings to the internal pores and vice versa.The well-developedmicropores and mesopores of PPAC respectively ensured the high adsorption of lithium polysulfides and a high rate ofion diffusion.Compared to CAC,PPAC is a high-performance,low-cost cathode material that is promising for use in futureLi–S batteries.
基金supported by the Fundamental Research Funds for the Provincial Universities (Grant No.2024-KYYWF-0141)the National Natural Science Foundation of China (Grant Nos.52406076,52227813)+1 种基金the National Key Research and Development Program of China (Grant No.2022YFE0133900)the China Postdoctoral Science Foundation (Grant No.2023M740905)。
文摘Visible lighting and energy-saving are dual needs of energy efficiency and occupant comfort in modern buildings.In this study,a smart window based on phase-change material VO_(2) is designed and optimized to address the critical challenges in building energy management.The proposed phase-adaptive radiative(PAR)coating is a multilayer nanostructure consisting of TiO/VO_(2)2/TiO/Ag_(2) and polydimethylsiloxane(PDMS).For different VO_(2) phases,visible transmittance T_(vis)>0.6 and emissivity difference in the atmospheric window Δε_(AW)=0.422 can be achieved,which means the PAR window can transfer interior heat to the outside through thermal radiation for cooling or minimize thermal emission for insulation,while ensuring the transmission of visible light for natural daylighting.Compared to normal glass,the PAR window has an average temperature drop of 14.8℃.The year-round energy-saving calculation for four different cities in China indicates that the PAR window can save 22%-32% of the annual cooling and heating energy consumption by seamlessly transitioning between two phases of VO_(2)modes.The multi-objective optimization of the phase-adaptive radiative smart window provides a potential strategy for energy saving.
基金supported by the Russian Science Foundation (No. 25-13-00300)the Centre for Optical and Laser Materials Research (SPbSU) and Saint Petersburg State University for measuring the quantum yields of luminescence within the framework of research project(No.125021902439-8)。
文摘The development of polymeric materials that exhibit blue thermally activated delayed fluorescence(TADF)is of great interest for optoelectronic applications.However,achieving TADF in polymers often requires an elaborate monomer design.The high-energy local triplet state(^(3)LE)of carbazole complicates its application despite the molecular orbital arrangement being suitable for blue emission.Here,we present an approach to polymer design that makes it possible to solve this problem.We demonstrate the in situ formation of a TADF donor-acceptor system during Suzuki polycondensation,creating an extended carbazole-based donor matrix coupled with a triazine acceptor.The resulting polymer exhibited efficient TADF with a low energy gap(ΔE_(ST))value if a phenyl N-substituent,enabling essential electron delocalization,was present in the carbazole moiety.This work establishes a versatile platform for developing carbazole-based TADF polymers,overcoming the fundamental limitations that hinder their widespread application.
基金supported by the National Natural Science Foundation of China(32130069)the National Key Research and Development Program of China(2024YFF1306700)the Scientific Research Foundation of Education Department of Yunnan Province(2024Y004).
文摘Soil greenhouse gas(GHG)emissions contribute profoundly to global warming;however,how plant detritus input alters GHG emissions is poorly understood.Here,we used detritus input and removal treatments(i.e.,DIRT:control,CK;double litter,DL;no roots with double litter,NRDL;no litter,NL;no roots,NR;no roots and no litter,NRNL)to assess the effects of litter and root inputs on soil CO_(2),CH_(4),and N_(2)O fluxes in soils in a coniferous(Pinus yunnanensis)and a broad-leaf forest(Quercus pannosa)in a subalpine region in southwestern China.Litter addition increased CO_(2) emissions on average 22.22%,but did not significantly alter CH_(4) uptake and N_(2)O emission compared to the CK.Litter removal(NL and NRNL)significantly reduced CO_(2) emissions on average 30.22%and N_(2)O emissions on average 31.16%from both forest soils,but did not significantly affect soil CH_(4) uptake.Root removal(NR and NRNL)generally decreased these three soil GHG fluxes.Changes inβ-1,4-glucosidase(BG)involved in C and phospholipid fatty acid(PLFAs)biomass were projected to influence CO_(2) emissions,while soil microclimates(temperature and moisture)combined with BG activity mainly regulated CH_(4) uptake.Alterations in dissolved organic nitrogen,microbial biomass nitrogen and BG were mainly responsible for changes in N_(2)O emissions.Interestingly,coniferous forest soil seemed to promote CH_(4) uptake more than the broad-leaf forest soil,but CO_(2) and N_(2)O fluxes were not significantly affected by the forest types.As expected,litter addition significantly increased the warming potential,while litter removal relatively lowered it.These findings revealed the divergent roles of plant detritus input and forest type in shaping soil GHG fluxes,thereby providing insights into forest management and predicting contributions of subalpine forests to global warming.
文摘A series of blue and blue‑green Ir(Ⅲ)complexes has been investigated theoretically to explore their electronic structures,photophysical properties,efficiency roll‑off effect,and thermal activation delayed fluorescence(TADF)properties.All calculations were performed using density functional theory(DFT)and time‑dependent density functional theory(TDDFT).Calculations for electronic structures,frontier molecular orbital characteristics(which determine the efficiency roll‑off effect of the complexes),and photophysical properties were conducted using the Gaussian 09 software package.The calculation of spin‑orbit coupling matrix elements<T|HSOC|S>,which determine the TADF properties of the complexes,was performed using the ORCA software package.The calculation results show that the auxiliary ligand tetraphenylimidodiphosphinate(tpip),a strong electron‑withdrawing group,can mitigate the efficiency roll‑off effect of the complex.Furthermore,TADF is observed in one of the designed complexes,(F_(3)Phppy)_(2)Ir(tpip),where F_(3)Phppy=2‑[4‑(2,4,6‑trifluorophenyl)phenyl]pyridine.
基金Innovation Team Development Program of the Ministry of Education:Research on the Prevention and Treatment of Cardiovascular Diseases with Traditional Chinese Medicine (IRT-16R54)。
文摘OBJECTIVE: Exploring the effect of Optimized New Shengmai powder(优化新生脉散方, ONSMP) on myocardial fibrosis in heart failure(HF) based on rat sarcoma(RAS)/rapidly accelerated fibrosarcoma(RAF)/mitogen-activated protein kinase kinase(MEK)/extracellular regulated protein kinases(ERK) signaling pathway. METHODS: Randomized 70 Sprague-Dawley rats into sham(n = 10) and operation(n = 60) groups, then established the HF rat by ligating the left anterior descending branch of the coronary artery. We randomly divided the operation group rats into the model, ONSMP [including low(L), medium(M), and high(H) dose], and enalapril groups. After the 4-week drug intervention, echocardiography examines the cardiac function and calculates the ratios of the whole/left heart to the rat's body weight. Finally, we observed the degree of myocardial fibrosis by pathological sections, determined myocardium collagen(COL) Ⅰ and COL Ⅲ content by enzyme-linked immunosorbent assay, detected the m RNA levels of COL Ⅰ, COL Ⅲ, α-smooth muscle actin(α-SMA), and c-Fos proto-oncogene(c-Fos) by universal real-time, and detected the protein expression of p-RAS, p-RAF, p-MEK1/2, p-ERK1/2, p-ETS-like-1 transcription factor(p-ELK1), p-c-Fos, α-SMA, COL Ⅰ, and COL Ⅲ by Western blot. RESULTS: ONSMP can effectively improve HF rat's cardiac function, decrease cardiac organ coefficient, COL volume fraction, and COL Ⅰ/Ⅲ content, down-regulate the m RNA of COL Ⅰ/Ⅲ, α-SMA and c-Fos, and the protein of p-RAS, p-RAF, p-MEK1/2, p-ERK1/2, p-ELK1, c-Fos, COL Ⅰ/Ⅲ, and α-SMA. CONCLUSIONS: ONSMP can effectively reduce myocardial fibrosis in HF rats, and the mechanism may be related to the inhibition of the RAS/RAF/MEK/ERK signaling pathway.
基金the National Key Research and Development Program of China(2022YFB3504503)the National Natural Science Foundation of China(52274355)the Gansu Province Science and Technology Major Special Project,China(22ZD6GD061).
文摘Precipitation is often used for the preparation of La(OH)_(3)with precipitants of liquid alkali and ammonia.To solve the problems of high cost and wastewater pollution caused by common precipitants,the active MgO synthesized by pyrolysis was used as the precipitant to prepare La(OH)_(3).The species distribution of LaCl_(3)and LaCl_(3)-MgCl_(2)mixed system solution was calculated,and the kinetic analysis of the precipi-tation process was carried out to confirm the key factors influencing the precipitation of La(OH)_(3).The results show that La(OH)_(3)with D_(50)of 5.57μm,a specific surface area of 25.70 m^(2)/g,a rod-like shape,and MgO content of 0.044 wt%,was successfully prepared by adding active MgO.The precipitation ratio of La reaches 99.92%.The La(OH)_(3)precipitation is controlled by the diffusion process.The activity of MgO has a significant influence on MgO content in the precipitate.The preparation of La(OH)_(3)by active MgO provides a potential way for an eco-friendly preparation method of rare earth.
基金supported by the National High Technology Research and Development Program of China(863 Program)(2012AA053305)the International Cooperation Project from Ministry of Science and Technology of China(2010DFA64080)
文摘Activated carbons derived from corncob （CACs） were prepared by pyrolysis carbonization and KOH activation. Through modifying activation conditions, samples with large pore volume and ultrahigh BET specific surface area could be obtained. The sample achieved the highest hydrogen uptake capacity of 5.80 wt% at 40 bar and -196℃ The as-obtained samples were characterized by N2-sorption, transmission electron microscopy （TEM） and scanning electron microscopy （SEM）. Besides, thermogravimetric analysis was also employed to investigate the activation behavior of CACs. Detailed investigation on the activation parameters reveals that moderate activation temperature and heating rate are favorable for preparing CACs with high surface area, large pore volume and optimal pore size distribution. Meanwhile, the micropore volume between 0.65 nm and 0.85 nm along with BET surface area and total pore volume has great effects on hydrogen uptake capacities. The present results indicate that CACs are the most promising materials for hydrogen storage application.
基金Basic Public Welfare Research Foundation of Zhejiang Province,China,No.GD21H290001and Traditional Chinese Medicine Science and Technology Project Foundation of Zhejiang Province,China,No.2020ZB072.
文摘BACKGROUND Sepsis is a major medical challenge.Magnolol is an active constituent of Houpu that improves tissue function and exerts strong anti-endotoxin and anti-inflammatory effects,but the mechanism by which it reduces intestinal inflammation in sepsis is yet unclear.AIM To assess the protective effect of magnolol on intestinal mucosal epithelial cells in sepsis and elucidate the underlying mechanisms.METHODS Enzyme-linked immunosorbent assay was used to measure tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),IL-6,and regulated on activation,normal T-cell expressed and secreted(RANTES)levels in serum and ileal tissue in animal studies.The histopathological changes of the ileal mucosa in different groups were observed under a microscope.Cell Counting Kit-8 and cell permeability assays were used to determine the concentration of drug-containing serum that did not affect the activity of Caco2 cells but inhibited lipopolysaccharide(LPS)-induced decrease in permeability.Immunofluorescence and Western blot assays were used to detect the levels of RANTES,inhibitor of nuclear factor kappa-B kinaseβ(IKKβ),phosphorylated IKKβ(p-IKKβ),inhibitor of nuclear factor kappa-B kinaseα(IκBα),p65,and p-p65 proteins in different groups in vitro.RESULTS In rats treated with LPS by intravenous tail injection in the presence or absence of magnolol,magnolol inhibited the expression of proinflammatory cytokines,IL-1β,IL-6,and TNF-αin a dose-dependent manner.In addition,magnolol suppressed the production of RANTES in LPS-stimulated sepsis rats.Moreover,in vitro studies suggested that magnolol inhibited the increase of p65 nucleation,thereby markedly downregulating the production of the phosphorylated form of IKKβin LPS-treated Caco2 cells.Specifically,magnolol inhibited the translocation of the transcription factor nuclear factor-kappa B(NF-κB)from the cytosol into the nucleus and down-regulated the expression level of the chemokine RANTES in LPS-stimulated Caco2 cells.CONCLUSION Magnolol down-regulates RANTES levels by inhibiting the LPS/NF-κB signaling pathways,thereby suppressing IL-1β,IL-6,and TNF-αexpression to alleviate the mucosal barrier dysfunction in sepsis.
基金This work was fnancially supported by National Natural Science Foundation of China(52074333,51874337)Taishan Scholar Foundation of Shandong Province(tspd20161004)Fundamental Research Funds for the Central Universities(19CX07001A).
文摘The ultra-low permeability reservoir is regarded as an important energy source for oil and gas resource development and is attracting more and more attention.In this work,the active silica nanofuids were prepared by modifed active silica nanoparticles and surfactant BSSB-12.The dispersion stability tests showed that the hydraulic radius of nanofuids was 58.59 nm and the zeta potential was−48.39 mV.The active nanofuids can simultaneously regulate liquid-liquid interface and solid-liquid interface.The nanofuids can reduce the oil/water interfacial tension(IFT)from 23.5 to 6.7 mN/m,and the oil/water/solid contact angle was altered from 42°to 145°.The spontaneous imbibition tests showed that the oil recovery of 0.1 wt%active nanofuids was 20.5%and 8.5%higher than that of 3 wt%NaCl solution and 0.1 wt%BSSB-12 solution.Finally,the efects of nanofuids on dynamic contact angle,dynamic interfacial tension and moduli were studied from the adsorption behavior of nanofuids at solid-liquid and liquid-liquid interface.The oil detaching and transporting are completed by synergistic efect of wettability alteration and interfacial tension reduction.The fndings of this study can help in better understanding of active nanofuids for EOR in ultra-low permeability reservoirs.
基金Supported by National Natural Science Foundation of China,No. 30750013
文摘AIM:To investigate the regulation of mindin expression and the signaling pathway involved during inflammation.METHODS:C57BL/6 mice were treated with 3% dextran sulfate sodium (DSS) in drinking water for 6 d to induce acute colitis,and then the colon was harvested for histological analysis or for RNA isolation.mRNA expression of mindin and nuclear factor (NF)-κB p65 was analyzed by quantitative real time polymerase chain reaction (RT-PCR) and mindin expression construct was conf irmed by Western blotting.Mouse macrophage and intestinal epithelial lineage cells were stimulated with different cytokines and toll-like receptor (TLR) ligands,before pNF-κB-luciferase activity was assessed using the Dual-Luciferase reporter assay system.RESULTS:mRNA expression of mindin was upregulated 4.7 ± 1.1 fold compared with the baseline during DSS-induced intestinal inflammation in the mice.Stimulation with CpG-ODN (a known TLR-9 ligand) induced 4.2 ± 0.3 fold upregulation of mindin expression in RAW 264.7 cells.Full-length of mindin was cloned from cDNA of mouse mesenteric lymph node,then the pCMV-Mindin-Flag expression vector was established and the protein expression level was confi rmed.Transfection of the mindin construct and stimulation with CpG-ODN signifi cantly increased the NF-κB-luciferase activity by 2.5 ± 0.3 and 4.5 ± 0.5 fold in RAW264.7 and CMT93 cells,respectively (P < 0.01).CONCLUSION:Mindin expression is upregulated during intestinal inflammation and may induce NF-κB promoter activation in a TLR-9 mediated manner.
文摘Taxation,the primary source of fiscal revenue,has profound implications in guiding resource allocation,promoting economic growth,adjusting social wealth distribution,and enhancing cultural influence.The development of e-taxation provides a enhanced security for taxation,but it still faces the risk of inefficiency and tax data leakage.As a decentralized ledger,blockchain provides an effective solution for protecting tax data and avoiding tax-related errors and fraud.The introduction of blockchain into e-taxation protocols can ensure the public verification of taxes.However,balancing taxpayer identity privacy with regulation remains a challenge.In this paper,we propose a blockchain-based anonymous and regulatory e-taxation protocol.This protocol ensures the supervision and tracking of malicious taxpayers while maintaining honest taxpayer identity privacy,reduces the storage needs for public key certificates in the public key infrastructure,and enables selfcertification of taxpayers’public keys and addresses.We formalize the security model of unforgeability for transactions,anonymity for honest taxpayers,and traceability for malicious taxpayers.Security analysis shows that the proposed protocol satisfies unforgeability,anonymity,and traceability.The experimental results of time consumption show that the protocol is feasible in practical applications.
文摘A plant fermentation was carried out by Lactobaccilli against the Rhizome from Pueraria milifica (f-PMF). This material was evidenced by safe in animal toxic study. The main aim of this study was to revise the traditional way of hot water extraction to fermentation so as to use up the original material and finding new activity. We tried to show the new activity through phytoestrogen and immune-competent cells from the host that administrated either of original remedy and the new fermented sample, plus activated water SRE. In mice, compromised host was prepared by cancer chemotherapeutic agent (Mitomycin-C). After administration of f-PMF to immno-suppressed animals, the effects by both samples were augmented by lymphocyte in number and functions, macrophage activities, anti-oxidative activity. However, the intense of effect was much more by fermented one but not by conventional one. The anti-oxidative assay was also carried out ex-vivo system by peritoneal macrophage that we proposed as suitable system for evaluating anti-oxidative assay. In our clinical study by 20 healthy volunteers, granulocyte and lymphocyte ratio was regulated as neutral in peripheral white blood cells, increasing one, two and three weeks after the administration of f-PMF. We have found the significant regulation of blood chemical factors that were important makers for the lifestyle-related diseases. The mechanism of augmentation by probing directory with immuno-electrophoretic method, generating new complement component, especially found by alternative pathway of complement. So we discussed the process concerning designed f-PMF molecule for activation of complement component and bound for the biological activity of each physical component. In a limited condition, fewer numbers of volunteers, the breast size was tending to increase along with the administration time. Including these evidences, we discussed the possibility of this traditional ethnic medicine, originally found and spread in the highland area in Thailand and Myanmar.
基金supported by the National Natural Science Foundation of China(No.52070149)Shaanxi Innovative Research Team for Key Science and Technology(No.2023-CXTD-36)+1 种基金Shaanxi Province Key Program for International S&T Cooperation Projects(No.2024GH-ZDXM-04)the Bureau of Science and Technology of Xi’an City of China(No.23SFSF0011).
文摘Anaerobic ammonia oxidation(Anammox)is an economical and sustainablewastewater nitrogen removal technology,and its application in the mainstream process is the inevitable trend of the development of Anammox.However,how to effectively enriching Anammox bacteria from the activated sludge remains challenging and restricts its extensive applications.In this study,the rapid and efficient enrichment of Anammox bacteriawas achieved by raising the reflux ratio and nitrogen loading rate(NLR)using conventional activated sludge as the inoculant.In the screening phase(days 1–90),the reflux ratio was increased to discharge partial floc sludge,resulting in the relative abundance of Candidatus Brocadiaceae increased from0.04%to 22.54%,which effectively reduced thematrix and spatial competition between other microorganisms and Anammox bacteria.On day 90,the stoichiometric ratio of the Anammox process closely approached the theoretical value of 1:1.32:0.26,indicating that the Anammox reaction was the primary nitrogen removal process in the system.In the enrichment phase(days 91–238),the NLR increased from 0.43 to 1.20 kgN/(m^(3)·d)and removal efficiency was 71.89%,resulting in the relative abundance of Candidatus Brocadiaceae increased to 61.27%on day 180.The reactor operated steadily from days 444 to 498,maintaining the nitrogen removal rate(NRR)of 3.00 kgN/(m^(3)·d)and achieving successful sludge granulation with the particle size of 392.4μm.In short,this study provided a simple and efficient approach for enriching Anammox bacteria from the activated sludge,supporting to start an Anammox process efficiently.
基金the National Natural Science Foundation of China(No.41807292)the Opening Fund of the State Key Laboratory of Geohazard Prevention and Geoenvironment Protection(Chengdu University of Technology)(Nos.SKLGP2017K001,SKLGP2018K003)。
文摘Due to the complex geological processes of Qinghai-Tibet Plateau,numerous deposits,especially the large-scale ancient landslide deposits,are characteristic features of the valleys incised in southwestern China.Intense water level fluctuations since 2011 in Maoergai Reservoir,China,registered the reactivation of Xierguazi ancient landslide,and presented a significant risk to neighboring facilities.Based on detailed field survey and drilling exploration,the landslide was divided into Zone A and Zone B,and other characterizations of landslide were studied as well.To precisely measure the extent of landslide displacement during filling and drawdown stage,surface displacement monitoring system was deployed on the landslide.The monitoring analyses data reveal that reservoir fluctuation is the dominant factor influencing landslide displacement,especially during drawdown stage.Moreover,a future sliding is anticipated in Zone A,while a creep had already existed in Zone B.A reservoir regulation was then established using the lead-lag correlation between reservoir fluctuation and landslide displacement and landslide stability analysis.In the end,the follow-up deformation monitoring demonstrates that the reservoir regulation controlled the landslide effectively.Landslide control by reservoir regulation in Maoergai can serve as a case study for other settlements involved in similar construction activities.
基金supported by Research Grants of the NRF(2023R1A2C2003823,RS-2024-00405818)funded by the National Research Foundation under the Ministry of Science,ICT&Future,Korea。
文摘Employing multiple metals for synergistic electronic structure regulation emerges as a promising approach to develop highly efficient and robust electrocatalysts for hydrogen evolution at ampere levels.In this study,a series of Schreibersite-type intermetallic compounds,particularly Mo_(2)Fe_(0.8)Ru_(0.2)P,are synthesized through high-temperature solid-phase synthesis.Experimental results demonstrate that the integration of Ru significantly improves the kinetics of proton adsorption and desorption during the hydrogen evolution reaction(HER).Additionally,density functional theory(DFT)calculations and X-ray absorption near edge structure(XANES)analyses effectively corroborate the pronounced d-orbital hybridization of Fe within the structure,which facilitates the transfer of hydroxide ions and the maintenance of material durability during alkaline HER processes.Remarkably,Mo_(2)Fe_(0.8)Ru_(0.2)P exhibits superior alkaline HER activity,characterized by an overpotential of merely 48 mV at a current density of 10 mA cm^(-2).After prolonged operation of 1000 h at high current densities(1.1 A cm^(-2)),the activity decline remains minimal,under 4%(with overpotential increasing from 258 mV to 268 mV).These results demonstrate the potential of strategically combining metallic elements to design high-performance industrial-grade electrocatalysts.
基金National Key Research and Development Program of China(2023YFB3810200 to J.L.)National Natural Science Foundation of China(92168204,82225030 to J.L.)Fundamental Research Funds for the Central Universities(22120210586 to J.L.)。
文摘Reproductive hormones associated with the hypothalamic-pituitary-gonadal(HPG)axis are closely linked to bone homeostasis.In this study,we demonstrate that Gonadotropin inhibitory hormone(GnIH,one of the key reproductive hormones upstream of the HPG axis)plays an indispensable role in regulating bone homeostasis and maintaining bone mass.We find that deficiency of GnIH or its receptor Gpr147 leads to a significant reduction in bone mineral density(BMD)in mice primarily by enhancement of osteoclast activation in vivo and in vitro.Mechanistically,GnIH/Gpr147 inhibits osteoclastogenesis by the PI3K/AKT,MAPK,NF-κB and Nfatc1 signaling pathways.Furthermore,GnIH treatment was able to alleviate bone loss in aging,ovariectomy(OVX)or LPS-induced mice.Moreover,the therapy using green light promotes the release of GnIH and rescues OVX-induced bone loss.In humans,serum GnIH increases and bone resorption markers decrease after green light exposure.Therefore,our study elucidates that GnIH plays an important role in maintaining bone homeostasis via modulating osteoclast differentiation and demonstrates the potential of GnIH therapy or green light therapy in preventing osteoporosis.
基金This work was supported by National Natural Science Foundation of China (No. 30070205 and No. 39730150).
文摘Swelling-activated Cl- currents, I(ci,swell), were measured during hyposmotic shock in white Leghorn embryonic chick heart cells using the whole-cell recording of patch-clamp technique. Genistein, an inhibitor of protein tyrosine kinase (PTK), suppressed I(ci,swell), Under isosmotic condition phorbol 12-myristate 13-acetate (PMA), an activator of PKC, elicited the Cl~ current similar to that in hyposmotic solution, whereas hyposmotic shock did not elicit I(ci,swell) in chelerythrine chloride(an inhibitor of PKC)-treated cells. Con-focal microscopy experiments using FITC-phalloidin as a fluorescent label of F-actin showed that the actin network was moved from cortical region of the cell to the center after hyposmotic shock as compared with the image under isosmotic condition. When the cells were treated with cytochalasin B (CB) or cytochalasin D (CD) under isosmotic condition the disruption of the F-actin integrity was observed, and I(ci,Sweii) was not elicited. With combination treatment of CB with PMA, hyposmotic solution could not elicited I(Ci,swell), The results suggested that the role of PTK, probably receptor tyrosine kinase, for regulation of I(ci,sweii) appeared to be at upstream site related to the role of F-actin. Then PKC signal pathway was activated somehow and finally change in the polymerization state of cytoskeleton led to activate the swelling-activated Cl- channels. These results demonstrate clearly that PTK, PKC and F-actin are important factors for regulation of I(Ci,swell) in embryonic chick heart cells as compared with often controversial results reported in different cell types.
