Taxation,the primary source of fiscal revenue,has profound implications in guiding resource allocation,promoting economic growth,adjusting social wealth distribution,and enhancing cultural influence.The development of...Taxation,the primary source of fiscal revenue,has profound implications in guiding resource allocation,promoting economic growth,adjusting social wealth distribution,and enhancing cultural influence.The development of e-taxation provides a enhanced security for taxation,but it still faces the risk of inefficiency and tax data leakage.As a decentralized ledger,blockchain provides an effective solution for protecting tax data and avoiding tax-related errors and fraud.The introduction of blockchain into e-taxation protocols can ensure the public verification of taxes.However,balancing taxpayer identity privacy with regulation remains a challenge.In this paper,we propose a blockchain-based anonymous and regulatory e-taxation protocol.This protocol ensures the supervision and tracking of malicious taxpayers while maintaining honest taxpayer identity privacy,reduces the storage needs for public key certificates in the public key infrastructure,and enables selfcertification of taxpayers’public keys and addresses.We formalize the security model of unforgeability for transactions,anonymity for honest taxpayers,and traceability for malicious taxpayers.Security analysis shows that the proposed protocol satisfies unforgeability,anonymity,and traceability.The experimental results of time consumption show that the protocol is feasible in practical applications.展开更多
On the basis of discussing the influencing mode of plant moisture stress on plant physiological process and the division of soil moisture availability range, the water suction values partitioning soil moisture were pu...On the basis of discussing the influencing mode of plant moisture stress on plant physiological process and the division of soil moisture availability range, the water suction values partitioning soil moisture were put forward, and then the corresponding water moistures under water stress were obtained by conversing together with characteristic curve of water moisture.展开更多
The development of high-performance structural and functional materials is vital in many industrial fields.High-and medium-entropy alloys(H/MEAs)with superior comprehensive properties owing to their specific microstru...The development of high-performance structural and functional materials is vital in many industrial fields.High-and medium-entropy alloys(H/MEAs)with superior comprehensive properties owing to their specific microstructures are promising candidates for structural materials.More importantly,multitudinous efforts have been made to regulate the microstructures and the properties of H/MEAs to further expand their industrial applications.The various heterostructures have enormous potential for the development of H/MEAs with outstanding performance.Herein,multiple heterogeneous structures with single and hierarchical heterogeneities were discussed in detail.Moreover,preparation methods for compositional inhomogeneity,bimodal structures,dualphase structures,lamella/layered structures,harmonic structures(core-shell),multiscale precipitates and heterostructures coupled with specific microstructures in H/MEAs were also systematically reviewed.The deformation mechanisms induced by the different heterostructures were thoroughly discussed to explore the relationship between the heterostructures and the optimized properties of H/MEAs.The contributions of the heterostructures and advanced microstructures to the H/MEAs were comprehensively elucidated to further improve the properties of the alloys.Finally,this review discussed the future challenges of high-performance H/MEAs for industrial applications and provides feasible methods for optimizing heterostructures to enhance the comprehensive properties of H/MEAs.展开更多
The orientation of precipitates was regulated by an improved twinning-aging-detwinning(TAD)process in Mg-Sn sheets.A structure with specific proportions of dual-precipitates was regulated,significantly enhancing the m...The orientation of precipitates was regulated by an improved twinning-aging-detwinning(TAD)process in Mg-Sn sheets.A structure with specific proportions of dual-precipitates was regulated,significantly enhancing the material’s strength.The formation mechanism of the dual-precipitates was investigated,and the evolution of precipitate orientation was confirmed.A combined SEM,EBSD and TEM method was developed to identify the coupling relationship between complex grain orientation and precipitate orientation.The orientation relationship between precipitates and the matrix could be quantitatively analyzed in the same region.Subsequently,the Orowan model was refined to quantify the strength enhancement effect following the orientation regulation of precipitates.The TAD sample containing dual-precipitates exhibited a 99.1%increase in TYS,equivalent to an increase of 108 MPa,compared to unregulated samples A.According to Orowan calculations,the theoretical strength increment from the three strengthening mechanisms was 113 MPa,closely matching the experimental increment of 108 MPa.Notably,the materials with a specific proportion of the dual-precipitates showed a substantial increase in strength.展开更多
To advance the precise regulation and high-value utilization of halloysite nanotubes(HNTs),this work systematically investigated five treatment strategies,including calcination,acid treatment,alkali treatment,acid tre...To advance the precise regulation and high-value utilization of halloysite nanotubes(HNTs),this work systematically investigated five treatment strategies,including calcination,acid treatment,alkali treatment,acid treatment of calcined HNTs,and alkali treatment of calcined HNTs,to modulate their structural and application properties.The structural characteristics,surface properties,and methylene blue(MB)adsorption capacity of HNTs under multiple treatments were systematically analyzed.Calcination at varying temperatures modified the crystal structure,morphology,and surface properties of HNTs,with higher calcination temperatures reducing their reactivity towards MB.Moderate acid treatment expanded the lumen and decreased the surface potential of HNTs,significantly enhancing MB adsorption capacity.In contrast,alkali treatment dispersed the multilayered walls of HNTs and raised surface potential,reducing MB affinity.Acid treatment of calcined HNTs effectively increased their specific surface areas by leaching most of Al while maintaining the tubular structure,thereby maximizing MB adsorption.Alkali treatment of calcined HNTs destroyed the tubular structure and resulted in poor MB adsorption.HNTs pre-calcined at 600℃ for 3 h and acid-treated at 60℃ for 8 h exhibited an optimal specific surface area of443 m^(2)·g^(-1)and an MB adsorption capacity of 190 mg·g^(-1).Kinetic and Arrhenius equation fittings indicated that chemical reactions control interactions of acids and alkalis with HNTs.This study provides a comprehensive comparison and analysis of five treatment methods,offering insights into regulating the structures and surface properties of HNTs by controlling the treatment condition,thereby laying a foundation for their efficient utilization in practical applications.展开更多
5-Aminolevulinic acid(ALA)is a natural plant growth regulator that promotes plant freezing tolerance.The WRKY family consists of plantspecific transcription factors(TFs)associated with abiotic stress responses.Up to n...5-Aminolevulinic acid(ALA)is a natural plant growth regulator that promotes plant freezing tolerance.The WRKY family consists of plantspecific transcription factors(TFs)associated with abiotic stress responses.Up to now,whether WRKYs are involved in ALA-induced plant freezing tolerance and the underlying mechanism is not clear.In this study,we found that pretreatment with 50 mg·L^(-1) ALA one week earlier significantly increased the freezing tolerance of nectarine(Prunus persica var.nectarina)pistils with higher antioxidant enzyme activity and osmotic solutes when the floral twigs were stressed by-3℃ for 6 h.ALA also enhanced the expression of PpWRKY18,PpCBF1,PpCOR1,and several genes encoding antioxidant enzymes(such as superoxide dismutase,peroxidase,and catalase)and pyrroline-5-carboxylate synthase(P5CS).When PpWRKY18 was overexpressed in tobacco,the transgenic plants exhibited greater freezing tolerance,which was further promoted by exogenous ALA.Y2H,Pull-down,BiFC,and LCI analyses revealed that PpWRKY18 interacts with PpCBF1,promoting the latter transcriptional activity.Additionally,Y1H experiments showed that PpWRKY18 directly binds to the promoter of PpPOD41 while PpCBF1 binds to the promoters of PpP5CS1 and PpCOR1,activating the target gene expressions.Furthermore,we established a yeast library using the promoter of PpWRKY18 as the bait to screen the upstream regulatory factors.By library screening,Y1H,DLR,and EMSA,we found that PpC3H37,a zinc finger protein,was responsive to chilling and ALA treatment,and as a transcription factor,it activated PpWRKY18 expression by directly binding to the promoter.Taken together,we reveal a regulatory network where ALA induces upregulation of PpC3H37 expression,which positively regulates PpWRKY18 expression.Subsequently,the regulatory pathway diverges into two branches.The first is CBF-dependent,where PpCBF1 interacts with PpWRKY18,binding the promoters of PpP5CS1 and PpCOR1.The second is CBF-independent,where PpWRKY18 directly binds the promoter of PpPOD41 to upregulate the gene expression and increase the antioxidant enzyme activity and freezing tolerance.These findings provide a novel insight of the mechanism of ALA in regulating the cold hardiness of nectarine pistil.展开更多
Root architecture is crucial for plant survival under drought,yet how water stress signals shape root development has remained unclear.Recently,Roy et al.(Science)revealed that drought-induced accumulation of reactive...Root architecture is crucial for plant survival under drought,yet how water stress signals shape root development has remained unclear.Recently,Roy et al.(Science)revealed that drought-induced accumulation of reactive oxygen species(ROS)in root nuclei triggers the auxin repressor IAA3 to form complexes(multimerization)that enable the recruitment of the co-repressor TOPLESS(TPL),thereby suppressing auxin signaling and blocking lateral root initiation.ROS signaling acts rapidly,within minutes of water deprivation,compared to slower ABA responses,allowing plants to quickly adjust lateral root growth.This discovery reveals a new redoxhormone regulatory mechanism that enables plants to fine-tune root branching during transient drought.Beyond advancing our understanding of adaptive root responses,these insights highlight potential molecular targets for developing crops with improved resilience under water limitation.展开更多
Constructing multi-dimensional hydrogen bond(H-bond)regulated single-molecule systems with multiemission remains a challenge.Herein,we report the design of a new excited-state intramolecular proton transfer(ESIPT)feat...Constructing multi-dimensional hydrogen bond(H-bond)regulated single-molecule systems with multiemission remains a challenge.Herein,we report the design of a new excited-state intramolecular proton transfer(ESIPT)featured chromophore(HBT-DPI)that shows flexible emission tunability via the multidimensional regulation of intra-and intermolecular H-bonds.The feature of switchable intramolecular Hbonds is induced via incorporating several hydrogen bond acceptors and donors into one single HBT-DPI molecule,allowing the“turn on/off”of ESIPT process by forming isomers with distinct intramolecular Hbonds configurations.In response to different external H-bonding environments,the obtained four types of crystal/cocrystals vary in the contents of isomers and the molecular packing modes,which are mainly guided by the intermolecular H-bonds,exhibiting non-emissive features or emissions ranging from green to orange.Utilizing the feature of intermolecular H-bond guided molecular packing,we demonstrate the utility of this fluorescent material for visualizing hydrophobic/hydrophilic areas on large-scale heterogeneous surfaces of modified poly(1,1-difluoroethylene)(PVDF)membranes and quantitatively estimating the surface hydrophobicity,providing a new approach for hydrophobicity/hydrophilicity monitoring and measurement.Overall,this study represents a new design strategy for constructing multi-dimensional hydrogen bond regulated ESIPT-based fluorescent materials that enable multiple emissions and unique applications.展开更多
Mitogen-activated protein kinases(MAPKs)are a family of proteins that constitute signaling pathways involved in processes that control gene expression,cell division, cell survival,apoptosis,metabolism,differentiation ...Mitogen-activated protein kinases(MAPKs)are a family of proteins that constitute signaling pathways involved in processes that control gene expression,cell division, cell survival,apoptosis,metabolism,differentiation and motility.The MAPK pathways can be divided into conventional and atypical MAPK pathways.The first group converts a signal into a cellular response through a relay of three consecutive phosphorylation events exerted by MAPK kinase kinases,MAPK kinase,and MAPK.Atypical MAPK pathways are not organized into this three-tiered cascade.MAPK that belongs to both conventional and atypical MAPK pathways can phosphorylate both non-protein kinase substrates and other protein kinases.The latter are referred to as MAPK-activated protein kinases.This review focuses on one such MAPK-activated protein kinase,MAPK-activated protein kinase 5(MK5)or p38-regulated/activated protein kinase(PRAK).This protein is highly conserved throughout the animal kingdom and seems to be the target of both conventional and atypical MAPK pathways.Recent findings on the regulation of the activity and subcellular localization,bona fide interaction partners and physiological roles of MK5/PRAK are discussed.展开更多
A DC regulated power supply with numerical control based on single chip microcomputer （SCM） is designed. SCM is the main controller and output voltage o{ DC power supply can be set by keyboard. The analog voltage ca...A DC regulated power supply with numerical control based on single chip microcomputer （SCM） is designed. SCM is the main controller and output voltage o{ DC power supply can be set by keyboard. The analog voltage can be obtained through D/A converter （DAC0832） so that different voltages can be provided by operational amplifier. The output voltage varies from 0 V to 12 V with the incremental value of 0. 1 V. The actual output voltage is shown in the nixietube. This DC regulated power supply is characterized by simple structure and easy operation.展开更多
With 7-year-old Korla fragrant pear trees as the experimental material, different root-zone irrigation patterns were arranged to study the effects of soil moisture on twig water potential of Korla fragrant pear trees ...With 7-year-old Korla fragrant pear trees as the experimental material, different root-zone irrigation patterns were arranged to study the effects of soil moisture on twig water potential of Korla fragrant pear trees at different growth stages. The results showed that under the condition of regulated deficit irrigation, the diurnal dynamics of twig water potential of Korla fragrant pear trees was V shaped at different growth stages, and the twig water potential of Korla fragrant pear trees reached the minimum during 14：00-16：00. At different growth stages, the twig water potential of Korla fragrant pear trees under drought stress was significantly lower than that of pear trees irrigated normally. Under both drought stress and normal irrigation, the diurnal variation of twig water potential of Korla fragrant pear trees during the flowering period was most gentle, that during the fruit-setting and mature periods showed some ups and downs, and that during the fruit enlargement period was greater. Under the experimental conditions, the twig water potential of Korla fragrant pear trees was positively correlated with soil moisture content, and the functional relationships between them at different growth stages were studied by regression analysis. In addition, the limits of twig water potential and soil moisture content for normal growth of Korla fragrant pear trees at different growth stages were determined.展开更多
This study demonstrates that our previously reported polywraplex, a synthetic siRNA carrier consisting of a uni-molecular polyplex core of customizable size and a self-assembled triblock copolymer envelop, may be cons...This study demonstrates that our previously reported polywraplex, a synthetic siRNA carrier consisting of a uni-molecular polyplex core of customizable size and a self-assembled triblock copolymer envelop, may be constructed using dendrimers as the crosslinking junctions. Replacing the branched low molecular weight PEI with polyamidoamine(PAMAM) dendrimer in the zeta potential regulated polymerization resulted in the similar network structured cationic polymer with electron microscopically visible crosslinking junctions. This visibility may offer a convenient way to characterize the molecular structure of the rationally designed networked siRNA-packing cationic polymer without altering its chemical properties and biologic functions. A series of physical-chemical characterizations and biological assays, comprising size, zeta potential, pre-phagocytic siRNA leaking and degradation, and silencing of functional genes, confirmed that the advanced properties of polywraplexes remained with the dendrimer junctions. Although sixth generation PAMAM dendrimer was used as the crosslinking junctions in the size-customizable polymerization for electron microscopic observation, lower generation dendrimer should also work in case more practical and structurally defined cationic polymer is needed.展开更多
On-road driving emissions of six liquefied natural gas(LNG) and diesel semi-trailer towing vehicles(STTVs) which met China Emission Standard IV and V were tested using Portable Emission Measurement System(PEMS) in nor...On-road driving emissions of six liquefied natural gas(LNG) and diesel semi-trailer towing vehicles(STTVs) which met China Emission Standard IV and V were tested using Portable Emission Measurement System(PEMS) in northern China.Emission characteristics of these vehicles under real driving conditions were analyzed and proved that on-road emissions of heavy-duty vehicles(HDVs) were underestimated in the past.There were large differences among LNG and diesel vehicles, which also existed between China V vehicles and China IV vehicles.Emission factors showed the highest level under real driving conditions, which probably be caused by frequent acceleration, deceleration, and start-stop.NOx emission factors ranged from 2.855 to 20.939 g/km based on distance-traveled and 6.719–90.557 g/kg based on fuel consumption during whole tests, which were much higher than previous researches on chassis dynamometer.It was inferred from tests that the fuel consumption rate of the test vehicles had a strong correlation with NOx emission, and the exhaust temperature also affected the efficiency of Selected Catalytic Reduction(SCR) aftertreatment system, thus changing the NOx emission greatly.THC emission factors of LNG vehicles were 2.012–10.636 g/km, which were much higher than that of diesel vehicles(0.029–0.185 g/km).Unburned CH4 may be an important reason for this phenomenon.Further on-road emission tests, especially CH4 emission test should be carried out in subsequent research.In addition, the Particulate Number(PN) emission factors of diesel vehicles were at a very high level during whole tests, and Diesel Particulate Filter(DPF)should be installed to reduce PN emission.展开更多
The effects of continuously regenerating diesel particulate filter （CRDPF） systems on regulated gaseous emissions, and number-size distribution and mass of particles emanated from a diesel engine have been investiga...The effects of continuously regenerating diesel particulate filter （CRDPF） systems on regulated gaseous emissions, and number-size distribution and mass of particles emanated from a diesel engine have been investigated in this study. Two CRDPF units （CRDPF-1 and CRDPF-2） with different specifications were separately retrofitted to the engine running with European steady-state cycle （ESC）. An electrical low pressure impactor （ELPI） was used for particle number-size distribution measurement and mass estimation. The conversion/reduction rate （RcR） of hydrocarbons （HC） and carbon monoxide （CO） across CRDPF-1 was 83% and 96.3%, respectively. Similarly, the RCR of HC and CO and across CRDPF-2 was 91.8% and 99.1%, respectively. The number concentration of particles and their concentration peaks; nuclei mode, accumulation mode and total particles; and particle mass were highly reduced with the CRDPF units. The nuclei mode particles at downstream of CRDPF-1 and CRDPF-2 decreased by 99.9% to 100% and 97.8% to 99.8% respectively; and the particle mass reduced by 73% to 92.2% and 35.3% to 72.4%, respectively, depending on the engine conditions. In addition, nuclei mode particles increased with the increasing of engine speed due to the heterogeneous nucleation initiated by the higher exhaust temperature, while accumulation mode particles were higher at higher loads due to the decrease in the air-to-fuel ratio （A/F） at higher loads.展开更多
BACKGROUND Sepsis is a major medical challenge.Magnolol is an active constituent of Houpu that improves tissue function and exerts strong anti-endotoxin and anti-inflammatory effects,but the mechanism by which it redu...BACKGROUND Sepsis is a major medical challenge.Magnolol is an active constituent of Houpu that improves tissue function and exerts strong anti-endotoxin and anti-inflammatory effects,but the mechanism by which it reduces intestinal inflammation in sepsis is yet unclear.AIM To assess the protective effect of magnolol on intestinal mucosal epithelial cells in sepsis and elucidate the underlying mechanisms.METHODS Enzyme-linked immunosorbent assay was used to measure tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),IL-6,and regulated on activation,normal T-cell expressed and secreted(RANTES)levels in serum and ileal tissue in animal studies.The histopathological changes of the ileal mucosa in different groups were observed under a microscope.Cell Counting Kit-8 and cell permeability assays were used to determine the concentration of drug-containing serum that did not affect the activity of Caco2 cells but inhibited lipopolysaccharide(LPS)-induced decrease in permeability.Immunofluorescence and Western blot assays were used to detect the levels of RANTES,inhibitor of nuclear factor kappa-B kinaseβ(IKKβ),phosphorylated IKKβ(p-IKKβ),inhibitor of nuclear factor kappa-B kinaseα(IκBα),p65,and p-p65 proteins in different groups in vitro.RESULTS In rats treated with LPS by intravenous tail injection in the presence or absence of magnolol,magnolol inhibited the expression of proinflammatory cytokines,IL-1β,IL-6,and TNF-αin a dose-dependent manner.In addition,magnolol suppressed the production of RANTES in LPS-stimulated sepsis rats.Moreover,in vitro studies suggested that magnolol inhibited the increase of p65 nucleation,thereby markedly downregulating the production of the phosphorylated form of IKKβin LPS-treated Caco2 cells.Specifically,magnolol inhibited the translocation of the transcription factor nuclear factor-kappa B(NF-κB)from the cytosol into the nucleus and down-regulated the expression level of the chemokine RANTES in LPS-stimulated Caco2 cells.CONCLUSION Magnolol down-regulates RANTES levels by inhibiting the LPS/NF-κB signaling pathways,thereby suppressing IL-1β,IL-6,and TNF-αexpression to alleviate the mucosal barrier dysfunction in sepsis.展开更多
BACKGROUND： Glucose-regulated protein 78 （GRP78）, a marker of endoplasmic reticulum stress, can prolong cell survival. Alternatively, CCAAT enhancer-binding protein homologous protein （CHOP）, a transcription fact...BACKGROUND： Glucose-regulated protein 78 （GRP78）, a marker of endoplasmic reticulum stress, can prolong cell survival. Alternatively, CCAAT enhancer-binding protein homologous protein （CHOP）, a transcription factor specific for endoplasmic reticulum stress, can cause cell cycle arrest and cell apoptosis. OBJECTIVE： To study the protective effects of serum containing natural cerebrolysin on endoplasmic reticulum stress in tunicamycin-induced neuronal PC12 cells, and analyze the influence on GRP78 and CHOP expressions. DESIGN, TIME AND SETTING： A parallel controlled study was performed at the Institute of Integrated Western and Traditional Chinese Medicine, Shenzhen Hospital, Southern Medical University, between March 2006 and August 2008. MATERIALS： Adult Sprague-Dawley rats were perfused with natural Cerebrolysin aqueous extract （0.185 g/kg/d） to produce serum containing natural Cerebrolysin. Physiological saline was used to produce blank serum. PC12 cell line was provided by Shanghai Institute of Cell Biology, Chinese Academy of Science. Tunicamycin was provided by Sigma （St. Louis, USA）, and natural Cerebrolysin, containing ginseng, rhizoma gastrodiae, and gingko leaf （1：2：2）, by Shengzhen Institute of Integrated Western and Traditional Chinese Medicine. METHODS： PC12 cells were treated with DMEM culture media containing 10% blank serum （normal control group）, tunicamycin （1 μg/mL; model group）, and 5%, 10%, and 15% serum containing natural cerebrolysin and tunicamycin （1 μ g/mL; low-, moderate-, and high-dose serum containing natural cerebrotysin groups）, for 2 hours. MAIN OUTCOME MEASURES： PC12 cells were treated with tunicamycin for 48 hours after which apoptosis was measured using the TUNEL method to calculate apoptotic index. GRP78 expression was detected using immunocytochemistry. After 24 hours of treatment with tunicamycin, GRP78 and CHOP mRNA expressions were measured using RT-PCR. RESULTS： The apoptotic index and CHOP mRNA expression were in the model group and three cerebrolysin groups were significantly increased when compared to the normal control group （P 〈 0.05）. In contrast, GRP78 mRNA and protein expressions were significantly decreased （P 〈 0.05）. CONCLUSION： Serum containing natural cerebrolysin significantly reduced apoptosis in neuronal PC12 cells following tunicamycin-induced endoplasmic reticulum stress. These results may be related to an up-regulation of GRP78 expression and down-regulation of CHOP expression, both of which displayed dose-dependent effects.展开更多
Genetically engineered transgenic animals and plants have proven to be extremely useful for analyzing biochemical and developmental processes.Promoters responding to chemical inducers will be powerful tools for basic ...Genetically engineered transgenic animals and plants have proven to be extremely useful for analyzing biochemical and developmental processes.Promoters responding to chemical inducers will be powerful tools for basic research in molecular biology and biotechnological applications.Various chemical inducible systems based on activation and inactivation of the target gene had been described.The transfer of regulatory elements from prokaryotes,insects,and mammals has opened new avenues to construct chemically inducible promoters that differ in their ability to regulate the temporal and spatial expression patterns,and this will dramatically increase the application of transgenic technology.This review provides an overview on regulation of gene expression,promoter activating systems,promoter inactivation systems,inducible gene over expression,and inducible anti suppression.展开更多
BACKGROUND Invasion and migration are the irreversible stages of colorectal cancer(CRC).The key is to find a sensitive,reliable molecular marker that can predict the migration of CRC at an early stage.N-myc downstream...BACKGROUND Invasion and migration are the irreversible stages of colorectal cancer(CRC).The key is to find a sensitive,reliable molecular marker that can predict the migration of CRC at an early stage.N-myc downstream regulated gene 1(NDRG1)is a multifunctional gene that has been tentatively reported to have a strong relationship with tumor invasion and migration,however the current molecular role of NDRG1 in CRC remains unknown.AIM To explore the role of NDRG1 in the development of CRC.METHODS NDRG1 stably over-expressed Caco2 cell line was established by lentiviral infection and NDRG1 knock-out Caco2 cell line was established by CRISPR/Cas9.Furthermore,the mRNA and protein levels of NDRG1 in Caco2 cells after NDRG1 over-expression and knockout were detected by real-time polymerase chain reaction and western blot.The cell proliferation rate was measured by the cell counting kit-8 method;cell cycle and apoptosis were detected by flow cytometry;invasion and migration ability were detected by the 24-transwell method.RESULTS NDRG1 over-expression inhibited Caco2 proliferation and the cell cycle could be arrested at the G1/S phase when NDRG1 was over-expressed,while the number of cells in the G2 phase was significantly increased when NDRG1 was knocked out.This suggests that NDRG1 inhibited the proliferation of Caco2 cells by arresting the cell cycle in the G1/S phase.Our data also demonstrated that NDRG1 promotes early cell apoptosis.Invasion and migration of cells were extensively inhibited when NDRG1 was over-expressed.CONCLUSION NDRG1 inhibits tumor progression in Caco2 cells which may represent a potential novel therapeutic strategy for the treatment of CRC.展开更多
Groundwater reservoir is a kind of important engineering, which can optimize water resources arran- gement by means of artificial regulation. Regulated water is the blood and value performance of groundwater reservoir...Groundwater reservoir is a kind of important engineering, which can optimize water resources arran- gement by means of artificial regulation. Regulated water is the blood and value performance of groundwater reservoir. To resolve the problem of real-time quantification of regulated water, the paper analyzed sources and compositions of regulated water in detail. Then, under the conditions of satisfying water demand inside research area, the paper analyzed quantity available and regulation coefficient of different regulated water and established a formula to calculate regulated water. At last, based on a pore groundwater reservoir in the middle reaches of the Yinma River, Jilin Province, the paper calculated regulated water with the formula and the result shows that the method is feasible. With some constraint conditions, the formula can be adopted in other similar areas.展开更多
Taking advantage of the fast-growing knowledge of RNA-binding proteins(RBPs)we review the signature of downregulated genes for RBPs in the transcriptome of induced pluripotent stem cell neurons(iNeurons)modelling the ...Taking advantage of the fast-growing knowledge of RNA-binding proteins(RBPs)we review the signature of downregulated genes for RBPs in the transcriptome of induced pluripotent stem cell neurons(iNeurons)modelling the neurodevelopmental Rubinstein Taybi Syndrome(RSTS)caused by mutations in the genes encoding CBP/p300 acetyltransferases.We discuss top and functionally connected downregulated genes sorted to“RNA processing”and“Ribonucleoprotein complex biogenesis”Gene Ontology clusters.The first set of downregulated RBPs includes members of hnRNHP(A1,A2B1,D,G,H2-H1,MAGOHB,PAPBC),core subunits of U small nuclear ribonucleoproteins and Serine-Arginine splicing regulators families,acting in precursor messenger RNA alternative splicing and processing.Consistent with literature findings on reduced transcript levels of serine/arginine repetitive matrix 4(SRRM4)protein,the main regulator of the neural-specific microexons splicing program upon depletion of Ep300 and Crebbp in mouse neurons,RSTS iNeurons show downregulated genes for proteins impacting this network.We link downregulated genes to neurological disorders including the new HNRNPH1-related intellectual disability syndrome with clinical overlap to RSTS.The set of downregulated genes for Ribosome biogenesis includes several components of ribosomal subunits and nucleolar proteins,such NOP58 and fibrillarin that form complexes with snoRNAs with a central role in guiding post-transcriptional modifications needed for rRNA maturation.These nucleolar proteins are“dual”players as fibrillarin is also required for epigenetic regulation of ribosomal genes and conversely NOP58-associated snoRNA levels are under the control of NOP58 interactor BMAL1,a transcriptional regulator of the circadian rhythm.Additional downregulated genes for“dual specificity”RBPs such as RUVBL1 and METTL1 highlight the links between chromatin and the RBP-ome and the contribution of perturbations in their cross-talk to RSTS.We underline the hub position of CBP/p300 in chromatin regulation,the impact of its defect on neurons’post-transcriptional regulation of gene expression and the potential use of epidrugs in therapeutics of RBP-caused neurodevelopmental disorders.展开更多
文摘Taxation,the primary source of fiscal revenue,has profound implications in guiding resource allocation,promoting economic growth,adjusting social wealth distribution,and enhancing cultural influence.The development of e-taxation provides a enhanced security for taxation,but it still faces the risk of inefficiency and tax data leakage.As a decentralized ledger,blockchain provides an effective solution for protecting tax data and avoiding tax-related errors and fraud.The introduction of blockchain into e-taxation protocols can ensure the public verification of taxes.However,balancing taxpayer identity privacy with regulation remains a challenge.In this paper,we propose a blockchain-based anonymous and regulatory e-taxation protocol.This protocol ensures the supervision and tracking of malicious taxpayers while maintaining honest taxpayer identity privacy,reduces the storage needs for public key certificates in the public key infrastructure,and enables selfcertification of taxpayers’public keys and addresses.We formalize the security model of unforgeability for transactions,anonymity for honest taxpayers,and traceability for malicious taxpayers.Security analysis shows that the proposed protocol satisfies unforgeability,anonymity,and traceability.The experimental results of time consumption show that the protocol is feasible in practical applications.
文摘On the basis of discussing the influencing mode of plant moisture stress on plant physiological process and the division of soil moisture availability range, the water suction values partitioning soil moisture were put forward, and then the corresponding water moistures under water stress were obtained by conversing together with characteristic curve of water moisture.
基金National Natural Science Foundation of China(52261032,51861021,51661016)Science and Technology Plan of Gansu Province(21YF5GA074)+2 种基金Public Welfare Project of Zhejiang Natural Science Foundation(LGG22E010008)Wenzhou Basic Public Welfare Scientific Research Project(G2023020)Incubation Program of Excellent Doctoral Dissertation-Lanzhou University of Technology。
文摘The development of high-performance structural and functional materials is vital in many industrial fields.High-and medium-entropy alloys(H/MEAs)with superior comprehensive properties owing to their specific microstructures are promising candidates for structural materials.More importantly,multitudinous efforts have been made to regulate the microstructures and the properties of H/MEAs to further expand their industrial applications.The various heterostructures have enormous potential for the development of H/MEAs with outstanding performance.Herein,multiple heterogeneous structures with single and hierarchical heterogeneities were discussed in detail.Moreover,preparation methods for compositional inhomogeneity,bimodal structures,dualphase structures,lamella/layered structures,harmonic structures(core-shell),multiscale precipitates and heterostructures coupled with specific microstructures in H/MEAs were also systematically reviewed.The deformation mechanisms induced by the different heterostructures were thoroughly discussed to explore the relationship between the heterostructures and the optimized properties of H/MEAs.The contributions of the heterostructures and advanced microstructures to the H/MEAs were comprehensively elucidated to further improve the properties of the alloys.Finally,this review discussed the future challenges of high-performance H/MEAs for industrial applications and provides feasible methods for optimizing heterostructures to enhance the comprehensive properties of H/MEAs.
基金National Natural Science Foundation of China(52374405)Joint Innovaion Fund Project of Lanzhou Jiaotong University and Corresponding Supporting University(LH2024018)State Key Laboratory Open Project(Q110824Q02001)。
文摘The orientation of precipitates was regulated by an improved twinning-aging-detwinning(TAD)process in Mg-Sn sheets.A structure with specific proportions of dual-precipitates was regulated,significantly enhancing the material’s strength.The formation mechanism of the dual-precipitates was investigated,and the evolution of precipitate orientation was confirmed.A combined SEM,EBSD and TEM method was developed to identify the coupling relationship between complex grain orientation and precipitate orientation.The orientation relationship between precipitates and the matrix could be quantitatively analyzed in the same region.Subsequently,the Orowan model was refined to quantify the strength enhancement effect following the orientation regulation of precipitates.The TAD sample containing dual-precipitates exhibited a 99.1%increase in TYS,equivalent to an increase of 108 MPa,compared to unregulated samples A.According to Orowan calculations,the theoretical strength increment from the three strengthening mechanisms was 113 MPa,closely matching the experimental increment of 108 MPa.Notably,the materials with a specific proportion of the dual-precipitates showed a substantial increase in strength.
基金Tural Science Foundation of China(No.52274255)the Young Scientists Fund of the National Natural ScienceFoundation of China(No.52404276)+3 种基金Fundamental Re-search Funds for the Central Universities,China(Nos.N2301003,N2201008,N2201004,and N2301025)Liaon-ingRevitalizationTalentsProgram,China(No.XLYC2202028)Postdoctoral Foundation of NortheasternUniversity,Young Elite Scientists Sponsorship Program byChina Association for Science and Technology(No.2022QNRC001)and China Postdoctoral Science Founda-tion(No.2022M720025)。
文摘To advance the precise regulation and high-value utilization of halloysite nanotubes(HNTs),this work systematically investigated five treatment strategies,including calcination,acid treatment,alkali treatment,acid treatment of calcined HNTs,and alkali treatment of calcined HNTs,to modulate their structural and application properties.The structural characteristics,surface properties,and methylene blue(MB)adsorption capacity of HNTs under multiple treatments were systematically analyzed.Calcination at varying temperatures modified the crystal structure,morphology,and surface properties of HNTs,with higher calcination temperatures reducing their reactivity towards MB.Moderate acid treatment expanded the lumen and decreased the surface potential of HNTs,significantly enhancing MB adsorption capacity.In contrast,alkali treatment dispersed the multilayered walls of HNTs and raised surface potential,reducing MB affinity.Acid treatment of calcined HNTs effectively increased their specific surface areas by leaching most of Al while maintaining the tubular structure,thereby maximizing MB adsorption.Alkali treatment of calcined HNTs destroyed the tubular structure and resulted in poor MB adsorption.HNTs pre-calcined at 600℃ for 3 h and acid-treated at 60℃ for 8 h exhibited an optimal specific surface area of443 m^(2)·g^(-1)and an MB adsorption capacity of 190 mg·g^(-1).Kinetic and Arrhenius equation fittings indicated that chemical reactions control interactions of acids and alkalis with HNTs.This study provides a comprehensive comparison and analysis of five treatment methods,offering insights into regulating the structures and surface properties of HNTs by controlling the treatment condition,thereby laying a foundation for their efficient utilization in practical applications.
基金supported by the Natural Science Foundation of China(Grant Nos.32230097 and 32172512)the Jiangsu Special Fund for Frontier Foundation Research of Carbon Peaking and Carbon Neutralization(Grant No.BK20220005)+1 种基金the Jiangsu Agricultural Science and Technology Innovation Fund[Grant No.CX(20)2023]a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.
文摘5-Aminolevulinic acid(ALA)is a natural plant growth regulator that promotes plant freezing tolerance.The WRKY family consists of plantspecific transcription factors(TFs)associated with abiotic stress responses.Up to now,whether WRKYs are involved in ALA-induced plant freezing tolerance and the underlying mechanism is not clear.In this study,we found that pretreatment with 50 mg·L^(-1) ALA one week earlier significantly increased the freezing tolerance of nectarine(Prunus persica var.nectarina)pistils with higher antioxidant enzyme activity and osmotic solutes when the floral twigs were stressed by-3℃ for 6 h.ALA also enhanced the expression of PpWRKY18,PpCBF1,PpCOR1,and several genes encoding antioxidant enzymes(such as superoxide dismutase,peroxidase,and catalase)and pyrroline-5-carboxylate synthase(P5CS).When PpWRKY18 was overexpressed in tobacco,the transgenic plants exhibited greater freezing tolerance,which was further promoted by exogenous ALA.Y2H,Pull-down,BiFC,and LCI analyses revealed that PpWRKY18 interacts with PpCBF1,promoting the latter transcriptional activity.Additionally,Y1H experiments showed that PpWRKY18 directly binds to the promoter of PpPOD41 while PpCBF1 binds to the promoters of PpP5CS1 and PpCOR1,activating the target gene expressions.Furthermore,we established a yeast library using the promoter of PpWRKY18 as the bait to screen the upstream regulatory factors.By library screening,Y1H,DLR,and EMSA,we found that PpC3H37,a zinc finger protein,was responsive to chilling and ALA treatment,and as a transcription factor,it activated PpWRKY18 expression by directly binding to the promoter.Taken together,we reveal a regulatory network where ALA induces upregulation of PpC3H37 expression,which positively regulates PpWRKY18 expression.Subsequently,the regulatory pathway diverges into two branches.The first is CBF-dependent,where PpCBF1 interacts with PpWRKY18,binding the promoters of PpP5CS1 and PpCOR1.The second is CBF-independent,where PpWRKY18 directly binds the promoter of PpPOD41 to upregulate the gene expression and increase the antioxidant enzyme activity and freezing tolerance.These findings provide a novel insight of the mechanism of ALA in regulating the cold hardiness of nectarine pistil.
文摘Root architecture is crucial for plant survival under drought,yet how water stress signals shape root development has remained unclear.Recently,Roy et al.(Science)revealed that drought-induced accumulation of reactive oxygen species(ROS)in root nuclei triggers the auxin repressor IAA3 to form complexes(multimerization)that enable the recruitment of the co-repressor TOPLESS(TPL),thereby suppressing auxin signaling and blocking lateral root initiation.ROS signaling acts rapidly,within minutes of water deprivation,compared to slower ABA responses,allowing plants to quickly adjust lateral root growth.This discovery reveals a new redoxhormone regulatory mechanism that enables plants to fine-tune root branching during transient drought.Beyond advancing our understanding of adaptive root responses,these insights highlight potential molecular targets for developing crops with improved resilience under water limitation.
基金supported by the National Key R&D Program of China(No.2021YFC2103600)the National Natural Science Foundation of China(Nos.21878156,21978131,22275085,and 22278224)+2 种基金the Natural Science Foundation of Jiangsu Province(Nos.BK20200089 and BK20200691)the Project of Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the State Key Laboratory of Materials-Oriented Chemical Engineering(No.KL21-08).
文摘Constructing multi-dimensional hydrogen bond(H-bond)regulated single-molecule systems with multiemission remains a challenge.Herein,we report the design of a new excited-state intramolecular proton transfer(ESIPT)featured chromophore(HBT-DPI)that shows flexible emission tunability via the multidimensional regulation of intra-and intermolecular H-bonds.The feature of switchable intramolecular Hbonds is induced via incorporating several hydrogen bond acceptors and donors into one single HBT-DPI molecule,allowing the“turn on/off”of ESIPT process by forming isomers with distinct intramolecular Hbonds configurations.In response to different external H-bonding environments,the obtained four types of crystal/cocrystals vary in the contents of isomers and the molecular packing modes,which are mainly guided by the intermolecular H-bonds,exhibiting non-emissive features or emissions ranging from green to orange.Utilizing the feature of intermolecular H-bond guided molecular packing,we demonstrate the utility of this fluorescent material for visualizing hydrophobic/hydrophilic areas on large-scale heterogeneous surfaces of modified poly(1,1-difluoroethylene)(PVDF)membranes and quantitatively estimating the surface hydrophobicity,providing a new approach for hydrophobicity/hydrophilicity monitoring and measurement.Overall,this study represents a new design strategy for constructing multi-dimensional hydrogen bond regulated ESIPT-based fluorescent materials that enable multiple emissions and unique applications.
文摘Mitogen-activated protein kinases(MAPKs)are a family of proteins that constitute signaling pathways involved in processes that control gene expression,cell division, cell survival,apoptosis,metabolism,differentiation and motility.The MAPK pathways can be divided into conventional and atypical MAPK pathways.The first group converts a signal into a cellular response through a relay of three consecutive phosphorylation events exerted by MAPK kinase kinases,MAPK kinase,and MAPK.Atypical MAPK pathways are not organized into this three-tiered cascade.MAPK that belongs to both conventional and atypical MAPK pathways can phosphorylate both non-protein kinase substrates and other protein kinases.The latter are referred to as MAPK-activated protein kinases.This review focuses on one such MAPK-activated protein kinase,MAPK-activated protein kinase 5(MK5)or p38-regulated/activated protein kinase(PRAK).This protein is highly conserved throughout the animal kingdom and seems to be the target of both conventional and atypical MAPK pathways.Recent findings on the regulation of the activity and subcellular localization,bona fide interaction partners and physiological roles of MK5/PRAK are discussed.
文摘A DC regulated power supply with numerical control based on single chip microcomputer （SCM） is designed. SCM is the main controller and output voltage o{ DC power supply can be set by keyboard. The analog voltage can be obtained through D/A converter （DAC0832） so that different voltages can be provided by operational amplifier. The output voltage varies from 0 V to 12 V with the incremental value of 0. 1 V. The actual output voltage is shown in the nixietube. This DC regulated power supply is characterized by simple structure and easy operation.
文摘With 7-year-old Korla fragrant pear trees as the experimental material, different root-zone irrigation patterns were arranged to study the effects of soil moisture on twig water potential of Korla fragrant pear trees at different growth stages. The results showed that under the condition of regulated deficit irrigation, the diurnal dynamics of twig water potential of Korla fragrant pear trees was V shaped at different growth stages, and the twig water potential of Korla fragrant pear trees reached the minimum during 14：00-16：00. At different growth stages, the twig water potential of Korla fragrant pear trees under drought stress was significantly lower than that of pear trees irrigated normally. Under both drought stress and normal irrigation, the diurnal variation of twig water potential of Korla fragrant pear trees during the flowering period was most gentle, that during the fruit-setting and mature periods showed some ups and downs, and that during the fruit enlargement period was greater. Under the experimental conditions, the twig water potential of Korla fragrant pear trees was positively correlated with soil moisture content, and the functional relationships between them at different growth stages were studied by regression analysis. In addition, the limits of twig water potential and soil moisture content for normal growth of Korla fragrant pear trees at different growth stages were determined.
基金the grant of the Natural Science Foundation of China(Grant nos.81373352 and 81690262)。
文摘This study demonstrates that our previously reported polywraplex, a synthetic siRNA carrier consisting of a uni-molecular polyplex core of customizable size and a self-assembled triblock copolymer envelop, may be constructed using dendrimers as the crosslinking junctions. Replacing the branched low molecular weight PEI with polyamidoamine(PAMAM) dendrimer in the zeta potential regulated polymerization resulted in the similar network structured cationic polymer with electron microscopically visible crosslinking junctions. This visibility may offer a convenient way to characterize the molecular structure of the rationally designed networked siRNA-packing cationic polymer without altering its chemical properties and biologic functions. A series of physical-chemical characterizations and biological assays, comprising size, zeta potential, pre-phagocytic siRNA leaking and degradation, and silencing of functional genes, confirmed that the advanced properties of polywraplexes remained with the dendrimer junctions. Although sixth generation PAMAM dendrimer was used as the crosslinking junctions in the size-customizable polymerization for electron microscopic observation, lower generation dendrimer should also work in case more practical and structurally defined cationic polymer is needed.
基金supported by the National Key Research and Development Program of China(No.2016YFC0208005)the Open Research Program of State Key Laboratory of Engine Combustion(No.K2018-11).
文摘On-road driving emissions of six liquefied natural gas(LNG) and diesel semi-trailer towing vehicles(STTVs) which met China Emission Standard IV and V were tested using Portable Emission Measurement System(PEMS) in northern China.Emission characteristics of these vehicles under real driving conditions were analyzed and proved that on-road emissions of heavy-duty vehicles(HDVs) were underestimated in the past.There were large differences among LNG and diesel vehicles, which also existed between China V vehicles and China IV vehicles.Emission factors showed the highest level under real driving conditions, which probably be caused by frequent acceleration, deceleration, and start-stop.NOx emission factors ranged from 2.855 to 20.939 g/km based on distance-traveled and 6.719–90.557 g/kg based on fuel consumption during whole tests, which were much higher than previous researches on chassis dynamometer.It was inferred from tests that the fuel consumption rate of the test vehicles had a strong correlation with NOx emission, and the exhaust temperature also affected the efficiency of Selected Catalytic Reduction(SCR) aftertreatment system, thus changing the NOx emission greatly.THC emission factors of LNG vehicles were 2.012–10.636 g/km, which were much higher than that of diesel vehicles(0.029–0.185 g/km).Unburned CH4 may be an important reason for this phenomenon.Further on-road emission tests, especially CH4 emission test should be carried out in subsequent research.In addition, the Particulate Number(PN) emission factors of diesel vehicles were at a very high level during whole tests, and Diesel Particulate Filter(DPF)should be installed to reduce PN emission.
基金supported by the National Natural Science Foundation of China (No. 40805053)
文摘The effects of continuously regenerating diesel particulate filter （CRDPF） systems on regulated gaseous emissions, and number-size distribution and mass of particles emanated from a diesel engine have been investigated in this study. Two CRDPF units （CRDPF-1 and CRDPF-2） with different specifications were separately retrofitted to the engine running with European steady-state cycle （ESC）. An electrical low pressure impactor （ELPI） was used for particle number-size distribution measurement and mass estimation. The conversion/reduction rate （RcR） of hydrocarbons （HC） and carbon monoxide （CO） across CRDPF-1 was 83% and 96.3%, respectively. Similarly, the RCR of HC and CO and across CRDPF-2 was 91.8% and 99.1%, respectively. The number concentration of particles and their concentration peaks; nuclei mode, accumulation mode and total particles; and particle mass were highly reduced with the CRDPF units. The nuclei mode particles at downstream of CRDPF-1 and CRDPF-2 decreased by 99.9% to 100% and 97.8% to 99.8% respectively; and the particle mass reduced by 73% to 92.2% and 35.3% to 72.4%, respectively, depending on the engine conditions. In addition, nuclei mode particles increased with the increasing of engine speed due to the heterogeneous nucleation initiated by the higher exhaust temperature, while accumulation mode particles were higher at higher loads due to the decrease in the air-to-fuel ratio （A/F） at higher loads.
基金Basic Public Welfare Research Foundation of Zhejiang Province,China,No.GD21H290001and Traditional Chinese Medicine Science and Technology Project Foundation of Zhejiang Province,China,No.2020ZB072.
文摘BACKGROUND Sepsis is a major medical challenge.Magnolol is an active constituent of Houpu that improves tissue function and exerts strong anti-endotoxin and anti-inflammatory effects,but the mechanism by which it reduces intestinal inflammation in sepsis is yet unclear.AIM To assess the protective effect of magnolol on intestinal mucosal epithelial cells in sepsis and elucidate the underlying mechanisms.METHODS Enzyme-linked immunosorbent assay was used to measure tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),IL-6,and regulated on activation,normal T-cell expressed and secreted(RANTES)levels in serum and ileal tissue in animal studies.The histopathological changes of the ileal mucosa in different groups were observed under a microscope.Cell Counting Kit-8 and cell permeability assays were used to determine the concentration of drug-containing serum that did not affect the activity of Caco2 cells but inhibited lipopolysaccharide(LPS)-induced decrease in permeability.Immunofluorescence and Western blot assays were used to detect the levels of RANTES,inhibitor of nuclear factor kappa-B kinaseβ(IKKβ),phosphorylated IKKβ(p-IKKβ),inhibitor of nuclear factor kappa-B kinaseα(IκBα),p65,and p-p65 proteins in different groups in vitro.RESULTS In rats treated with LPS by intravenous tail injection in the presence or absence of magnolol,magnolol inhibited the expression of proinflammatory cytokines,IL-1β,IL-6,and TNF-αin a dose-dependent manner.In addition,magnolol suppressed the production of RANTES in LPS-stimulated sepsis rats.Moreover,in vitro studies suggested that magnolol inhibited the increase of p65 nucleation,thereby markedly downregulating the production of the phosphorylated form of IKKβin LPS-treated Caco2 cells.Specifically,magnolol inhibited the translocation of the transcription factor nuclear factor-kappa B(NF-κB)from the cytosol into the nucleus and down-regulated the expression level of the chemokine RANTES in LPS-stimulated Caco2 cells.CONCLUSION Magnolol down-regulates RANTES levels by inhibiting the LPS/NF-κB signaling pathways,thereby suppressing IL-1β,IL-6,and TNF-αexpression to alleviate the mucosal barrier dysfunction in sepsis.
基金Supported by:Scientific and Technological Foundation of the National Administration of Traditional Chinese Medicine of China,No.02-03LP41the Scientific and Technological Key Project of Guangdong Province,No. 2006B35630007
文摘BACKGROUND： Glucose-regulated protein 78 （GRP78）, a marker of endoplasmic reticulum stress, can prolong cell survival. Alternatively, CCAAT enhancer-binding protein homologous protein （CHOP）, a transcription factor specific for endoplasmic reticulum stress, can cause cell cycle arrest and cell apoptosis. OBJECTIVE： To study the protective effects of serum containing natural cerebrolysin on endoplasmic reticulum stress in tunicamycin-induced neuronal PC12 cells, and analyze the influence on GRP78 and CHOP expressions. DESIGN, TIME AND SETTING： A parallel controlled study was performed at the Institute of Integrated Western and Traditional Chinese Medicine, Shenzhen Hospital, Southern Medical University, between March 2006 and August 2008. MATERIALS： Adult Sprague-Dawley rats were perfused with natural Cerebrolysin aqueous extract （0.185 g/kg/d） to produce serum containing natural Cerebrolysin. Physiological saline was used to produce blank serum. PC12 cell line was provided by Shanghai Institute of Cell Biology, Chinese Academy of Science. Tunicamycin was provided by Sigma （St. Louis, USA）, and natural Cerebrolysin, containing ginseng, rhizoma gastrodiae, and gingko leaf （1：2：2）, by Shengzhen Institute of Integrated Western and Traditional Chinese Medicine. METHODS： PC12 cells were treated with DMEM culture media containing 10% blank serum （normal control group）, tunicamycin （1 μg/mL; model group）, and 5%, 10%, and 15% serum containing natural cerebrolysin and tunicamycin （1 μ g/mL; low-, moderate-, and high-dose serum containing natural cerebrotysin groups）, for 2 hours. MAIN OUTCOME MEASURES： PC12 cells were treated with tunicamycin for 48 hours after which apoptosis was measured using the TUNEL method to calculate apoptotic index. GRP78 expression was detected using immunocytochemistry. After 24 hours of treatment with tunicamycin, GRP78 and CHOP mRNA expressions were measured using RT-PCR. RESULTS： The apoptotic index and CHOP mRNA expression were in the model group and three cerebrolysin groups were significantly increased when compared to the normal control group （P 〈 0.05）. In contrast, GRP78 mRNA and protein expressions were significantly decreased （P 〈 0.05）. CONCLUSION： Serum containing natural cerebrolysin significantly reduced apoptosis in neuronal PC12 cells following tunicamycin-induced endoplasmic reticulum stress. These results may be related to an up-regulation of GRP78 expression and down-regulation of CHOP expression, both of which displayed dose-dependent effects.
文摘Genetically engineered transgenic animals and plants have proven to be extremely useful for analyzing biochemical and developmental processes.Promoters responding to chemical inducers will be powerful tools for basic research in molecular biology and biotechnological applications.Various chemical inducible systems based on activation and inactivation of the target gene had been described.The transfer of regulatory elements from prokaryotes,insects,and mammals has opened new avenues to construct chemically inducible promoters that differ in their ability to regulate the temporal and spatial expression patterns,and this will dramatically increase the application of transgenic technology.This review provides an overview on regulation of gene expression,promoter activating systems,promoter inactivation systems,inducible gene over expression,and inducible anti suppression.
基金the National Natural Science Foundation of China,No.81260361Incubation Project of Mianyang Central Hospital,No.2020FH05.
文摘BACKGROUND Invasion and migration are the irreversible stages of colorectal cancer(CRC).The key is to find a sensitive,reliable molecular marker that can predict the migration of CRC at an early stage.N-myc downstream regulated gene 1(NDRG1)is a multifunctional gene that has been tentatively reported to have a strong relationship with tumor invasion and migration,however the current molecular role of NDRG1 in CRC remains unknown.AIM To explore the role of NDRG1 in the development of CRC.METHODS NDRG1 stably over-expressed Caco2 cell line was established by lentiviral infection and NDRG1 knock-out Caco2 cell line was established by CRISPR/Cas9.Furthermore,the mRNA and protein levels of NDRG1 in Caco2 cells after NDRG1 over-expression and knockout were detected by real-time polymerase chain reaction and western blot.The cell proliferation rate was measured by the cell counting kit-8 method;cell cycle and apoptosis were detected by flow cytometry;invasion and migration ability were detected by the 24-transwell method.RESULTS NDRG1 over-expression inhibited Caco2 proliferation and the cell cycle could be arrested at the G1/S phase when NDRG1 was over-expressed,while the number of cells in the G2 phase was significantly increased when NDRG1 was knocked out.This suggests that NDRG1 inhibited the proliferation of Caco2 cells by arresting the cell cycle in the G1/S phase.Our data also demonstrated that NDRG1 promotes early cell apoptosis.Invasion and migration of cells were extensively inhibited when NDRG1 was over-expressed.CONCLUSION NDRG1 inhibits tumor progression in Caco2 cells which may represent a potential novel therapeutic strategy for the treatment of CRC.
基金Under the auspices of Scientific & Technological Development Project of Science & Technology Office, JilinProvince (No. 200104032)
文摘Groundwater reservoir is a kind of important engineering, which can optimize water resources arran- gement by means of artificial regulation. Regulated water is the blood and value performance of groundwater reservoir. To resolve the problem of real-time quantification of regulated water, the paper analyzed sources and compositions of regulated water in detail. Then, under the conditions of satisfying water demand inside research area, the paper analyzed quantity available and regulation coefficient of different regulated water and established a formula to calculate regulated water. At last, based on a pore groundwater reservoir in the middle reaches of the Yinma River, Jilin Province, the paper calculated regulated water with the formula and the result shows that the method is feasible. With some constraint conditions, the formula can be adopted in other similar areas.
基金This work was supported by Italian Ministery of Health RC 08C921 to LL,Istituto Auxologico Italiano,IRCCs.
文摘Taking advantage of the fast-growing knowledge of RNA-binding proteins(RBPs)we review the signature of downregulated genes for RBPs in the transcriptome of induced pluripotent stem cell neurons(iNeurons)modelling the neurodevelopmental Rubinstein Taybi Syndrome(RSTS)caused by mutations in the genes encoding CBP/p300 acetyltransferases.We discuss top and functionally connected downregulated genes sorted to“RNA processing”and“Ribonucleoprotein complex biogenesis”Gene Ontology clusters.The first set of downregulated RBPs includes members of hnRNHP(A1,A2B1,D,G,H2-H1,MAGOHB,PAPBC),core subunits of U small nuclear ribonucleoproteins and Serine-Arginine splicing regulators families,acting in precursor messenger RNA alternative splicing and processing.Consistent with literature findings on reduced transcript levels of serine/arginine repetitive matrix 4(SRRM4)protein,the main regulator of the neural-specific microexons splicing program upon depletion of Ep300 and Crebbp in mouse neurons,RSTS iNeurons show downregulated genes for proteins impacting this network.We link downregulated genes to neurological disorders including the new HNRNPH1-related intellectual disability syndrome with clinical overlap to RSTS.The set of downregulated genes for Ribosome biogenesis includes several components of ribosomal subunits and nucleolar proteins,such NOP58 and fibrillarin that form complexes with snoRNAs with a central role in guiding post-transcriptional modifications needed for rRNA maturation.These nucleolar proteins are“dual”players as fibrillarin is also required for epigenetic regulation of ribosomal genes and conversely NOP58-associated snoRNA levels are under the control of NOP58 interactor BMAL1,a transcriptional regulator of the circadian rhythm.Additional downregulated genes for“dual specificity”RBPs such as RUVBL1 and METTL1 highlight the links between chromatin and the RBP-ome and the contribution of perturbations in their cross-talk to RSTS.We underline the hub position of CBP/p300 in chromatin regulation,the impact of its defect on neurons’post-transcriptional regulation of gene expression and the potential use of epidrugs in therapeutics of RBP-caused neurodevelopmental disorders.
