The scientific community is continuously working to translate the novel biomedical techniques into effective medical treatments.CRISPR-Cas9 system(Clustered Regularly Interspaced Short Palindromic Repeats-9),commonly ...The scientific community is continuously working to translate the novel biomedical techniques into effective medical treatments.CRISPR-Cas9 system(Clustered Regularly Interspaced Short Palindromic Repeats-9),commonly known as the“molecular scissor”,represents a recently developed biotechnology able to improve the quality and the efficacy of traditional treatments,related to several human diseases,such as chronic diseases,neurodegenerative pathologies and,interestingly,oral diseases.Of course,dental medicine has notably increased the use of biotechnologies to ensure modern and conservative approaches:in this landscape,the use of CRISPR-Cas9 system may speed and personalize the traditional therapies,ensuring a good predictability of clinical results.The aim of this critical overview is to provide evidence on CRISPR efficacy,taking into specific account its applications in oral medicine.展开更多
In this paper, we consider the general quasi-differential expressions each of order n with complex coefficients and their formal adjoints on the interval (a,b). It is shown in direct sum spaces of functions defined on...In this paper, we consider the general quasi-differential expressions each of order n with complex coefficients and their formal adjoints on the interval (a,b). It is shown in direct sum spaces of functions defined on each of the separate intervals with the cases of one and two singular end-points and when all solutions of the equation and its adjoint are in (the limit circle case) that all well-posed extensions of the minimal operator have resolvents which are HilbertSchmidt integral operators and consequently have a wholly discrete spectrum. This implies that all the regularly solvable operators have all the standard essential spectra to be empty. These results extend those of formally symmetric expression studied in [1-10] and those of general quasi-differential expressions in [11-19].展开更多
Pulse diagnosis is an important basis for the diagnosis of Chinese medicine.It is the rich experience accumulated by the predecessors in the long and continuous practice,and it is a unique diagnosis method of Chinese ...Pulse diagnosis is an important basis for the diagnosis of Chinese medicine.It is the rich experience accumulated by the predecessors in the long and continuous practice,and it is a unique diagnosis method of Chinese medicine.There are many names of pulse conditions in traditional Chinese medicine.The four elements are analyzed and summarized in terms of position,number,shape,and momentum.The four elements are used as 28 kinds of pulses.Among them,the regularly intermittent pulse and the intermittent pulse are characterized by arrhythmia and intermittent stop of the pulse.Intermittent pulse means irregular intervals;when a pulse comes,Regularly Intermittent pulse have regular stops.This kind of phenomenon is similar to the bradyarrhythmia in modern medicine.The knot pulse is an irregular pulse stopping,which can be seen clinically in sinus arrest,second-degree type I sinus block,second-degree type I atrioventricular block,and escape beats and so on.On the other hand,Regularly Intermittent pulse is a regular pulse stopping,which can be clinically found in the second degree type II sinus block,the second degree type II sinus block,and the second degree type I atrioventricular block with a special 2:1 type of atrioventricular block type and so on.展开更多
Studies of wave-current interactions are vital for the safe design of structures.Regular waves in the presence of uniform,linear shear,and quadratic shear currents are explored by the High-Level Green-Naghdi model in ...Studies of wave-current interactions are vital for the safe design of structures.Regular waves in the presence of uniform,linear shear,and quadratic shear currents are explored by the High-Level Green-Naghdi model in this paper.The five-point central difference method is used for spatial discretization,and the fourth-order Adams predictor-corrector scheme is employed for marching in time.The domain-decomposition method is applied for the wave-current generation and absorption.The effects of currents on the wave profile and velocity field are examined under two conditions:the same velocity of currents at the still-water level and the constant flow volume of currents.Wave profiles and velocity fields demonstrate substantial differences in three types of currents owing to the diverse vertical distribution of current velocity and vorticity.Then,loads on small-scale vertical cylinders subjected to regular waves and three types of background currents with the same flow volume are investigated.The maximum load intensity and load fluctuation amplitude in uniform,linear shear,and quadratic shear currents increase sequentially.The stretched superposition method overestimates the maximum load intensity and load fluctuation amplitude in opposing currents and underestimates these values in following currents.The stretched superposition method obtains a poor approximation for strong nonlinear waves,particularly in the case of the opposing quadratic shear current.展开更多
For the multiplicative background risk model,a distortion-type risk measure is used to measure the tail risk of the portfolio under a scenario probability measure with multivariate regular variation.In this paper,we i...For the multiplicative background risk model,a distortion-type risk measure is used to measure the tail risk of the portfolio under a scenario probability measure with multivariate regular variation.In this paper,we investigate the tail asymptotics of the portfolio loss ∑_(i=1)^(d)R_(i)S,where the stand-alone risk vector R=(R_(1),...,R_(d))follows a multivariate regular variation and is independent of the background risk factor S.An explicit asymptotic formula is established for the tail distortion risk measure,and an example is given to illustrate our obtained results.展开更多
A Turkish national,known in Chinese as Tang Xiaoqiang,has been confirmed to have died in the earthquake that struck Turkey and Syria on Monday,February 6,2023.
BACKGROUND In recent years,many studies have shown that proteasome 26S subunit non-ATPase 6(PSMD6)plays an important role in the occurrence and development of malignant tumours.Unfortunately,there are no reports on th...BACKGROUND In recent years,many studies have shown that proteasome 26S subunit non-ATPase 6(PSMD6)plays an important role in the occurrence and development of malignant tumours.Unfortunately,there are no reports on the evaluation of the potential role of PSMD6 in hepatocellular carcinoma(HCC).AIM To comprehensively evaluate the overexpression pattern and clinical significance of PSMD6 in HCC tissues.METHODS This study integrated PSMD6 mRNA expression profiles from 4672 HCC and 3667 non-HCC tissues,along with immunohistochemical scores from 383 HCC and adjacent tissues,to assess PSMD6 overexpression in HCC.Clustered regularly interspaced short palindromic repeats knockout technology evaluated PSMD6’s essential role in HCC cell growth.Functional enrichment analysis explored the molecular mechanism of PSMD6 abnormalities in HCC.Drug sensitivity analysis and molecular docking analysed the effect of abnormal expression of PSMD6 on the drug sensitivity of HCC cells.RESULTS The results of 41 external and two internal datasets showed that PSMD6 mRNA(SMD=0.26,95%CI:0.09-0.42,P<0.05)and protein(SMD=2.85,95%CI:1.19-4.50,P<0.05)were significantly overexpressed in HCC tissues.The integrated analysis results showed that PSMD6 had a significant overexpression pattern in HCC tissues(SMD=0.40,95%CI:0.15-0.66,P<0.05).PSMD6 knockout inhibited HCC cell growth(chronos scores<-1).Functional enrichment implicated ribosome biogenesis and RNA splicing.Significant enrichment of signalling pathways such as RNA degradation,ribosomes,and chemical carcinogenesis—reactive oxygen species.Drug sensitivity analysis and a molecular docking model showed that high expression of PSMD6 was associated with the tolerance of HCC cells to drugs such as ML323,sepantronium bromide,and GDC0810.Overexpressed PSMD6 effectively distinguished HCC tissues(AUC=0.75,95%CI:0.71-0.79).CONCLUSION This study was the first to discover that PSMD6 was overexpressed in HCC tissues.PSMD6 is essential for the growth of HCC cells and may be involved in ribosome biogenesis and RNA splicing.展开更多
Gastric cancer(GC)remains one of the leading causes of cancer-related mortality worldwide,necessitating innovative approaches for its diagnosis and treatment.Clustered regularly interspaced short palindromic repeats(C...Gastric cancer(GC)remains one of the leading causes of cancer-related mortality worldwide,necessitating innovative approaches for its diagnosis and treatment.Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPRassociated protein 9(Cas9),a revolutionary gene-editing technology,has emerged as a powerful tool for unraveling the molecular mechanisms underlying GC and for advancing precision medicine strategies.This review explores the current applications of CRISPR/Cas9 in GC research,including the identification of oncogenes and tumor suppressors,modeling tumor microenvironment interactions,and developing gene-based therapies.We highlight recent breakthroughs in genome editing that have enhanced our understanding of GC pathogenesis and resistance mechanisms to conventional therapies.Additionally,we discuss the potential of CRISPR/Cas9 for therapeutic gene editing in GC,addressing challenges such as off-target effects,delivery methods,and ethical considerations.By summarizing the progress and limitations of CRISPR/Cas9 in GC,this review aims to provide a comprehensive perspective on how this transformative technology could shape future strategies for the prevention,diagnosis,and treatment of GC.展开更多
A recent study by Qin et al emphasized the potential of zinc finger protein 71(ZNF71)as a promising biomarker for hepatocellular carcinoma(HCC).The authors offered valuable insights into the relationship between ZNF71...A recent study by Qin et al emphasized the potential of zinc finger protein 71(ZNF71)as a promising biomarker for hepatocellular carcinoma(HCC).The authors offered valuable insights into the relationship between ZNF71 and various clinical and pathological stages of HCC.However,several limitations are required to be addressed to improve the findings.These limitations include concerns regarding patient selection,the generalizability of the results,and the necessity for functional validation to establish ZNF71’s specific role in the progression of HCC.Furthermore,statistical issues related to multiple comparisons,confounding variables,and the inherent heterogeneity of high-throughput datasets warrant careful consideration.Future research should focus on multi-institutional cohorts,utilize in vivo models,and compare ZNF71 with established biomarkers to strengthen the clinical relevance of ZNF71.展开更多
BACKGROUND Although thymopoietin(TMPO)has been elucidated to be overexpressed in cancers,its underlying mechanisms are not yet fully understood.AIM To investigate the expression and clinical significance of TMPO in pa...BACKGROUND Although thymopoietin(TMPO)has been elucidated to be overexpressed in cancers,its underlying mechanisms are not yet fully understood.AIM To investigate the expression and clinical significance of TMPO in papillary thyroid carcinoma(PTC).METHODS Databases such as Gene Expression Omnibus,The Cancer Genome Atlas Proand summary receiver operating characteristic curves were plotted to evaluate diagnostic performance.A Gene Set Enrichment Analysis enrichment analysis was conducted to identify TMPO-related signaling pathways.A protein interaction network was constructed to identify hub genes.The impact of TMPO on PTC cell proliferation and the effects of its knockout were analyzed using clustered regularly interspaced short palindromic repeats(CRISPR)knockout screening and the Cancer Cell Line Encyclopedia database.RESULTS The TMPO protein was significantly overexpressed in PTC tissues,primarily localized in the cytoplasm and nuclear membrane.The mRNA level analysis showed mild overexpression of TMPO in PTC tissues,with a certain discriminatory value(area under the curve=0.66).TMPO may promote cancer through involvement in cell adhesion,focal adhesion,leukocyte migration,and multiple cancer-related signaling pathways.Additionally,CRISPR gene knockout experiments confirmed that TMPO knockout significantly inhibited the proliferation of PTC cell lines,indicating its important role in tumor growth.CONCLUSION TMPO is overexpressed in PTC and may serve as a therapeutic target and molecular biomarker for PTC.展开更多
BACKGROUND The prevalence of colorectal cancer(CRC)in younger people is increasing.Despite advances in precision medicine,the challenges of drug resistance and high costs persist.Nitidine chloride(NC)has pharmacologic...BACKGROUND The prevalence of colorectal cancer(CRC)in younger people is increasing.Despite advances in precision medicine,the challenges of drug resistance and high costs persist.Nitidine chloride(NC)has pharmacological potential,and kinesin family member 20A(KIF20A)is overexpressed in various tumors;however,their interaction in CRC remains unexplored.AIM To investigate the KIF20A expression characteristics in CRC cells and determine whether it is a potential target gene for NC in inhibiting CRC treatment.METHODS Single-cell RNA sequencing(scRNA-seq),spatial transcriptomics,and mRNA expression profiling were used to analyze KIF20A expression in CRC cells.Immunohistochemical staining was used to verify KIF20A expression in 416 clinical samples(208 CRC tissue samples and 208 noncancerous control tissue samples).Clustered regularly interspaced short palindromic repeats(CRISPR)technology was used to evaluate the impact of knocking out KIF20A on CRC cell growth.Molecular docking was applied to analyze NC–KIF20A binding.Finally,RNA sequencing and functional enrichment analysis were performed to explore the mechanism of action of NC in CRC cells.RESULTS Treating HCT116 cells with NC was found to significantly downregulate KIF20A(P<0.05),and the molecular docking analysis revealed high-affinity binding between NC and KIF20A(binding energy=-9.6 kcal/mol).The scRNA-seq,spatial transcriptomics,and mRNA expression profiling results confirmed the significantly high expression of KIF20A in CRC tissues(standardized mean difference=1.33,95%confidence interval:0.885-1.77,summary receiver operating characteristic curve area=0.94).The immunohistochemical analysis of the clinical samples showed high KIF20A expression in the CRC tissues(P<0.05),with significant correlation between the level of expression and gender,tumor size,and tumor grade(P<0.05).Knocking out KIF20A significantly inhibited the growth of various CRC cell lines(CRISPR score<-0.3).The functional enrichment analysis indicated that NC may inhibit CRC by disrupting several biological processes,such as mitotic nuclear division,chromosome segregation,and microtubule binding.CONCLUSION Our results indicate that NC binds to KIF20A with high affinity and downregulates its expression in CRC cells,leading to reduced proliferation.Hence,NC has promise as a therapeutic agent in the treatment of CRC,and targeting KIF20A also has potential as a therapeutic strategy.Further KIF20A knockout studies are needed to confirm the binding specificity and mechanistic roles of NC in CRC.展开更多
BACKGROUND Hepatocellular carcinoma(HCC)remains a lethal malignancy due to its molecular complexity and chemoresistance.Rac family small GTPase 3(RAC3),a tumorigenic GTPase understudied in HCC,drives recurrence via E2...BACKGROUND Hepatocellular carcinoma(HCC)remains a lethal malignancy due to its molecular complexity and chemoresistance.Rac family small GTPase 3(RAC3),a tumorigenic GTPase understudied in HCC,drives recurrence via E2F transcription factor 1(E2F1)-mediated transcriptional activation.This study integrates multiomics and clustered regularly interspaced short palindromic repeats(CRISPR)screening to delineate RAC3’s roles.RAC3 overexpression correlates with advanced HCC and patient age,while its knockout suppresses proliferation.Mechanistically,RAC3 dysregulates cell-cycle checkpoints through E2F1 binding.Pharmacological RAC3 inhibition disrupts tumor growth and synergizes with chemotherapy to overcome resistance.AIM To explore RAC3’s expression,clinical links,and HCC mechanisms via multiomics and functional genomics.METHODS Multiomic integration of The Cancer Genome Atlas(TCGA),Gene Expression Omnibus,and Genotype-Tissue Expression datasets was performed to analyze RAC3 mRNA expression.Immunohistochemistry quantified RAC3 protein in 108 HCC/adjacent tissue pairs.Kaplan–Meier/Cox regression assessed prognostic significance using TCGA data.CRISPR screening validated RAC3’s necessity for HCC proliferation.Functional enrichment identified associated pathways;hTFtarget/JASPAR predicted transcription factors,validated via chromatin immunoprecipitation sequencing(ChIP-seq).RESULTS RAC3 exhibited significant mRNA and protein overexpression in HCC tissues,which was correlated with advanced tumor stages and reduced overall survival rates(hazard ratio=1.82,95%CI:1.31–2.53).Genetic ablation of RAC3 suppressed HCC cell proliferation across 16 cell lines.Pathway analysis revealed RAC3’s predominant involvement in cell-cycle regulation,DNA replication,and nucleocytoplasmic transport.Mechanistic investigations identified E2F1 as a pivotal upstream transcriptional regulator,and ChIP-seq analysis validated its direct binding to the RAC3 promoter region.These findings suggest that RAC3 drives HCC progression through E2F1-mediated cell-cycle dysregulation.CONCLUSION This study identified RAC3 as a key HCC oncogenic driver;its overexpression links to poor prognosis/resistance.Targeting the RAC3/E2F1 axis offers a new therapy,which highlights RAC3 as a biomarker/target.展开更多
An H-polygon is a polygon with corners in the set of vertices of a tiling of R~2 by regular hexagons of unit edge.It is known that any H-triangle with exactly k interior H-points can have at most 3k+7 boundary H-point...An H-polygon is a polygon with corners in the set of vertices of a tiling of R~2 by regular hexagons of unit edge.It is known that any H-triangle with exactly k interior H-points can have at most 3k+7 boundary H-points.In this note we prove that any convex H-quadrilateral with exactly k interior H-points can have at most 3k+7 boundary H-points.展开更多
The finite volume method was applied to numerically simulate the bottom pressure field induced by regular waves,vehicles in calm water and vehicles in regular waves.The solution of Navier-Stokes(N-S)equations in the v...The finite volume method was applied to numerically simulate the bottom pressure field induced by regular waves,vehicles in calm water and vehicles in regular waves.The solution of Navier-Stokes(N-S)equations in the vicinity of numerical wave tank's boundary was forced towards the wave theoretical solution by incorporating momentum source terms,thereby reducing adverse effects such as wave reflection.Simulations utilizing laminar flow,turbulent flow,and ideal fluid models were all found capable of effectively capturing the waveform and bottom pressure of regular waves,agreeing well with experimental data.In predicting the bottom pressure field of the submerged vehicle,turbulent simulations considering fluid viscosity and boundary layer development provided more accurate predictions for the stern region than inviscid simulations.Due to sphere's diffractive effect,the sphere's bottom pressure field in waves is not a linear superposition of the wave's and the sphere's bottom pressure field.However,a slender submerged vehicle exhibits a weaker diffractive effect on waves,thus the submerged vehicle's bottom pressure field in waves can be approximated as a linear superposition of the wave's and the submerged vehicle's bottom pressure field,which simplifies computation and analysis.展开更多
On a compact Riemann surface with finite punctures P_(1),…P_(k),we define toric curves as multivalued,totallyunramified holomorphic maps to P^(n)with monodromy in a maximal torus of PSU(n+1).Toric solutions to SU(n+1...On a compact Riemann surface with finite punctures P_(1),…P_(k),we define toric curves as multivalued,totallyunramified holomorphic maps to P^(n)with monodromy in a maximal torus of PSU(n+1).Toric solutions to SU(n+1)Todasystems on X\{P_(1);…;P_(k)}are recognized by the associated toric curves in.We introduce character n-ensembles as-tuples of meromorphic one-forms with simple poles and purely imaginary periods,generating toric curves on minus finitelymany points.On X,we establish a correspondence between character-ensembles and toric solutions to the SU(n+1)system with finitely many cone singularities.Our approach not only broadens seminal solutions with two conesingularities on the Riemann sphere,as classified by Jost-Wang(Int.Math.Res.Not.,2002,(6):277-290)andLin-Wei-Ye(Invent.Math.,2012,190(1):169-207),but also advances beyond the limits of Lin-Yang-Zhong’s existencetheorems(J.Differential Geom.,2020,114(2):337-391)by introducing a new solution class.展开更多
The prevalence of Solenopsis invicta is associated with habitat diversity,with a preference for various habitats ranked in the following order:ponds,wastelands,green belts,roads,parks,farmlands,and villages.Consequent...The prevalence of Solenopsis invicta is associated with habitat diversity,with a preference for various habitats ranked in the following order:ponds,wastelands,green belts,roads,parks,farmlands,and villages.Consequently,management strategies should be tailored to each habitat.There are two occurrence peaks of S.invicta throughout the year,specifically from March to April and from September to October.It is essential to emphasize prevention and control measures during the active periods of S.invicta.To enhance the effectiveness of these measures,supplementary control should be implemented within 10-15 d following the initial prevention and control efforts.Furthermore,the efficacy of prevention and control is notably higher on the second to third day immediately following rainfall.This study aimed to evaluate the efficacy of various agents in controlling S.invicta.A comparative analysis was conducted on the efficacy of three different brands of agents against S.invicta.The findings indicated that a concentration of 0.08%-0.10%indoxacarb exhibited a comparable extermination effect on S.invicta worker ants.However,agents with larger particle sizes demonstrated a reduced efficacy in exterminating S.invicta nests.展开更多
Toric patch is a kind of rational multisided patch,which is associated with a finite integer lattice points set A.A set of weights is defined which depend on a parameter according to regular decomposition of A.When al...Toric patch is a kind of rational multisided patch,which is associated with a finite integer lattice points set A.A set of weights is defined which depend on a parameter according to regular decomposition of A.When all weights of the patch tend to infinity,we obtain the limiting form of toric patch which is called its regular control surface.The diferent weights may induce the diferent regular control surfaces of the same toric patch.It prompts us to consider that how many regular control surfaces of a toric patch.In this paper,we study the regular decompositions of A by using integer programming method firstly,and then provide the relationship between all regular decompositions of A and corresponding state polytope.Moreover,we present that the number of regular control surfaces of a toric patch associated with A is equal to the number of regular decompositions of A.An algorithm to calculate the number of regular control surfaces of toric patch is provided.The algorithm also presents a method to construct all of the regular control surfaces of a toric patch.At last,the application of proposed result in shape deformation is demonstrated by several examples.展开更多
Absorption compensation is a process involving the exponential amplification of reflection amplitudes.This process amplifies the seismic signal and noise,thereby substantially reducing the signal-tonoise ratio of seis...Absorption compensation is a process involving the exponential amplification of reflection amplitudes.This process amplifies the seismic signal and noise,thereby substantially reducing the signal-tonoise ratio of seismic data.Therefore,this paper proposes a multichannel inversion absorption compensation method based on structure tensor regularization.First,the structure tensor is utilized to extract the spatial inclination of seismic signals,and the spatial prediction filter is designed along the inclination direction.The spatial prediction filter is then introduced into the regularization condition of multichannel inversion absorption compensation,and the absorption compensation is realized under the framework of multichannel inversion theory.The spatial predictability of seismic signals is also introduced into the objective function of absorption compensation inversion.Thus,the inversion system can effectively suppress the noise amplification effect during absorption compensation and improve the recovery accuracy of high-frequency signals.Synthetic and field data tests are conducted to demonstrate the accuracy and effectiveness of the proposed method.展开更多
Energy resolution calibration is crucial for gamma-ray spectral analysis,as measured using a scintillation detector.A locally constrained regularization method was proposed to determine the resolution calibration para...Energy resolution calibration is crucial for gamma-ray spectral analysis,as measured using a scintillation detector.A locally constrained regularization method was proposed to determine the resolution calibration parameters.First,a Monte Carlo simulation model consistent with an actual measurement system was constructed to obtain the energy deposition distribution in the scintillation crystal.Subsequently,the regularization objective function is established based on weighted least squares and additional constraints.Additional constraints were designed using a special weighting scheme based on the incident gamma-ray energies.Subsequently,an intelligent algorithm was introduced to search for the optimal resolution calibration parameters by minimizing the objective function.The most appropriate regularization parameter was determined through mathematical experiments.When the regularization parameter was 30,the calibrated results exhibited the minimum RMSE.Simulations and test pit experiments were conducted to verify the performance of the proposed method.The simulation results demonstrate that the proposed algorithm can determine resolution calibration parameters more accurately than the traditional weighted least squares,and the test pit experimental results show that the R-squares between the calibrated and measured spectra are larger than 0.99.The accurate resolution calibration parameters determined by the proposed method lay the foundation for gamma-ray spectral processing and simulation benchmarking.展开更多
文摘The scientific community is continuously working to translate the novel biomedical techniques into effective medical treatments.CRISPR-Cas9 system(Clustered Regularly Interspaced Short Palindromic Repeats-9),commonly known as the“molecular scissor”,represents a recently developed biotechnology able to improve the quality and the efficacy of traditional treatments,related to several human diseases,such as chronic diseases,neurodegenerative pathologies and,interestingly,oral diseases.Of course,dental medicine has notably increased the use of biotechnologies to ensure modern and conservative approaches:in this landscape,the use of CRISPR-Cas9 system may speed and personalize the traditional therapies,ensuring a good predictability of clinical results.The aim of this critical overview is to provide evidence on CRISPR efficacy,taking into specific account its applications in oral medicine.
文摘In this paper, we consider the general quasi-differential expressions each of order n with complex coefficients and their formal adjoints on the interval (a,b). It is shown in direct sum spaces of functions defined on each of the separate intervals with the cases of one and two singular end-points and when all solutions of the equation and its adjoint are in (the limit circle case) that all well-posed extensions of the minimal operator have resolvents which are HilbertSchmidt integral operators and consequently have a wholly discrete spectrum. This implies that all the regularly solvable operators have all the standard essential spectra to be empty. These results extend those of formally symmetric expression studied in [1-10] and those of general quasi-differential expressions in [11-19].
基金National Natural Science Foundation of China(81874404)。
文摘Pulse diagnosis is an important basis for the diagnosis of Chinese medicine.It is the rich experience accumulated by the predecessors in the long and continuous practice,and it is a unique diagnosis method of Chinese medicine.There are many names of pulse conditions in traditional Chinese medicine.The four elements are analyzed and summarized in terms of position,number,shape,and momentum.The four elements are used as 28 kinds of pulses.Among them,the regularly intermittent pulse and the intermittent pulse are characterized by arrhythmia and intermittent stop of the pulse.Intermittent pulse means irregular intervals;when a pulse comes,Regularly Intermittent pulse have regular stops.This kind of phenomenon is similar to the bradyarrhythmia in modern medicine.The knot pulse is an irregular pulse stopping,which can be seen clinically in sinus arrest,second-degree type I sinus block,second-degree type I atrioventricular block,and escape beats and so on.On the other hand,Regularly Intermittent pulse is a regular pulse stopping,which can be clinically found in the second degree type II sinus block,the second degree type II sinus block,and the second degree type I atrioventricular block with a special 2:1 type of atrioventricular block type and so on.
基金Supported by the Development and Application Project of Ship CAE Software.
文摘Studies of wave-current interactions are vital for the safe design of structures.Regular waves in the presence of uniform,linear shear,and quadratic shear currents are explored by the High-Level Green-Naghdi model in this paper.The five-point central difference method is used for spatial discretization,and the fourth-order Adams predictor-corrector scheme is employed for marching in time.The domain-decomposition method is applied for the wave-current generation and absorption.The effects of currents on the wave profile and velocity field are examined under two conditions:the same velocity of currents at the still-water level and the constant flow volume of currents.Wave profiles and velocity fields demonstrate substantial differences in three types of currents owing to the diverse vertical distribution of current velocity and vorticity.Then,loads on small-scale vertical cylinders subjected to regular waves and three types of background currents with the same flow volume are investigated.The maximum load intensity and load fluctuation amplitude in uniform,linear shear,and quadratic shear currents increase sequentially.The stretched superposition method overestimates the maximum load intensity and load fluctuation amplitude in opposing currents and underestimates these values in following currents.The stretched superposition method obtains a poor approximation for strong nonlinear waves,particularly in the case of the opposing quadratic shear current.
基金supported by the National Key Research and Development Plan(No.2016YFC0800100)the NSFC of China(Nos.11671374,71771203,71631006).
文摘For the multiplicative background risk model,a distortion-type risk measure is used to measure the tail risk of the portfolio under a scenario probability measure with multivariate regular variation.In this paper,we investigate the tail asymptotics of the portfolio loss ∑_(i=1)^(d)R_(i)S,where the stand-alone risk vector R=(R_(1),...,R_(d))follows a multivariate regular variation and is independent of the background risk factor S.An explicit asymptotic formula is established for the tail distortion risk measure,and an example is given to illustrate our obtained results.
文摘A Turkish national,known in Chinese as Tang Xiaoqiang,has been confirmed to have died in the earthquake that struck Turkey and Syria on Monday,February 6,2023.
基金Supported by National Natural Science Foundation of China,No.82160762Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine Scientific Research Project,No.GXZYA20230267+2 种基金China Undergraduate Innovation and Entrepreneurship Training Program,No.S202410598060XChina Undergraduate Innovation and Entrepreneurship Training Program,No.X202410598360Future Academic Star of Guangxi Medical University,No.WLXSZX24074.
文摘BACKGROUND In recent years,many studies have shown that proteasome 26S subunit non-ATPase 6(PSMD6)plays an important role in the occurrence and development of malignant tumours.Unfortunately,there are no reports on the evaluation of the potential role of PSMD6 in hepatocellular carcinoma(HCC).AIM To comprehensively evaluate the overexpression pattern and clinical significance of PSMD6 in HCC tissues.METHODS This study integrated PSMD6 mRNA expression profiles from 4672 HCC and 3667 non-HCC tissues,along with immunohistochemical scores from 383 HCC and adjacent tissues,to assess PSMD6 overexpression in HCC.Clustered regularly interspaced short palindromic repeats knockout technology evaluated PSMD6’s essential role in HCC cell growth.Functional enrichment analysis explored the molecular mechanism of PSMD6 abnormalities in HCC.Drug sensitivity analysis and molecular docking analysed the effect of abnormal expression of PSMD6 on the drug sensitivity of HCC cells.RESULTS The results of 41 external and two internal datasets showed that PSMD6 mRNA(SMD=0.26,95%CI:0.09-0.42,P<0.05)and protein(SMD=2.85,95%CI:1.19-4.50,P<0.05)were significantly overexpressed in HCC tissues.The integrated analysis results showed that PSMD6 had a significant overexpression pattern in HCC tissues(SMD=0.40,95%CI:0.15-0.66,P<0.05).PSMD6 knockout inhibited HCC cell growth(chronos scores<-1).Functional enrichment implicated ribosome biogenesis and RNA splicing.Significant enrichment of signalling pathways such as RNA degradation,ribosomes,and chemical carcinogenesis—reactive oxygen species.Drug sensitivity analysis and a molecular docking model showed that high expression of PSMD6 was associated with the tolerance of HCC cells to drugs such as ML323,sepantronium bromide,and GDC0810.Overexpressed PSMD6 effectively distinguished HCC tissues(AUC=0.75,95%CI:0.71-0.79).CONCLUSION This study was the first to discover that PSMD6 was overexpressed in HCC tissues.PSMD6 is essential for the growth of HCC cells and may be involved in ribosome biogenesis and RNA splicing.
文摘Gastric cancer(GC)remains one of the leading causes of cancer-related mortality worldwide,necessitating innovative approaches for its diagnosis and treatment.Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPRassociated protein 9(Cas9),a revolutionary gene-editing technology,has emerged as a powerful tool for unraveling the molecular mechanisms underlying GC and for advancing precision medicine strategies.This review explores the current applications of CRISPR/Cas9 in GC research,including the identification of oncogenes and tumor suppressors,modeling tumor microenvironment interactions,and developing gene-based therapies.We highlight recent breakthroughs in genome editing that have enhanced our understanding of GC pathogenesis and resistance mechanisms to conventional therapies.Additionally,we discuss the potential of CRISPR/Cas9 for therapeutic gene editing in GC,addressing challenges such as off-target effects,delivery methods,and ethical considerations.By summarizing the progress and limitations of CRISPR/Cas9 in GC,this review aims to provide a comprehensive perspective on how this transformative technology could shape future strategies for the prevention,diagnosis,and treatment of GC.
文摘A recent study by Qin et al emphasized the potential of zinc finger protein 71(ZNF71)as a promising biomarker for hepatocellular carcinoma(HCC).The authors offered valuable insights into the relationship between ZNF71 and various clinical and pathological stages of HCC.However,several limitations are required to be addressed to improve the findings.These limitations include concerns regarding patient selection,the generalizability of the results,and the necessity for functional validation to establish ZNF71’s specific role in the progression of HCC.Furthermore,statistical issues related to multiple comparisons,confounding variables,and the inherent heterogeneity of high-throughput datasets warrant careful consideration.Future research should focus on multi-institutional cohorts,utilize in vivo models,and compare ZNF71 with established biomarkers to strengthen the clinical relevance of ZNF71.
基金Supported by Guangxi Zhuang Autonomous Region Health Commission Scientific Research Project,No.Z-A20220521Guangxi Higher Education Undergraduate Teaching Reform Project,No.2022JGA147The National College Students’Innovation and Entrepreneurship Training Program,No.202310598042.
文摘BACKGROUND Although thymopoietin(TMPO)has been elucidated to be overexpressed in cancers,its underlying mechanisms are not yet fully understood.AIM To investigate the expression and clinical significance of TMPO in papillary thyroid carcinoma(PTC).METHODS Databases such as Gene Expression Omnibus,The Cancer Genome Atlas Proand summary receiver operating characteristic curves were plotted to evaluate diagnostic performance.A Gene Set Enrichment Analysis enrichment analysis was conducted to identify TMPO-related signaling pathways.A protein interaction network was constructed to identify hub genes.The impact of TMPO on PTC cell proliferation and the effects of its knockout were analyzed using clustered regularly interspaced short palindromic repeats(CRISPR)knockout screening and the Cancer Cell Line Encyclopedia database.RESULTS The TMPO protein was significantly overexpressed in PTC tissues,primarily localized in the cytoplasm and nuclear membrane.The mRNA level analysis showed mild overexpression of TMPO in PTC tissues,with a certain discriminatory value(area under the curve=0.66).TMPO may promote cancer through involvement in cell adhesion,focal adhesion,leukocyte migration,and multiple cancer-related signaling pathways.Additionally,CRISPR gene knockout experiments confirmed that TMPO knockout significantly inhibited the proliferation of PTC cell lines,indicating its important role in tumor growth.CONCLUSION TMPO is overexpressed in PTC and may serve as a therapeutic target and molecular biomarker for PTC.
基金Supported by the Promoting Project of Basic Capacity for Young and Middle-aged University Teachers in Guangxi,No.2025KY0164Youth Science Foundation of Guangxi Medical University,No.GXMUYSF202423Guangxi Zhuang Autonomous Region Health Commission Scientific Research Project,No.Z-A20220415 and No.Z20210442.
文摘BACKGROUND The prevalence of colorectal cancer(CRC)in younger people is increasing.Despite advances in precision medicine,the challenges of drug resistance and high costs persist.Nitidine chloride(NC)has pharmacological potential,and kinesin family member 20A(KIF20A)is overexpressed in various tumors;however,their interaction in CRC remains unexplored.AIM To investigate the KIF20A expression characteristics in CRC cells and determine whether it is a potential target gene for NC in inhibiting CRC treatment.METHODS Single-cell RNA sequencing(scRNA-seq),spatial transcriptomics,and mRNA expression profiling were used to analyze KIF20A expression in CRC cells.Immunohistochemical staining was used to verify KIF20A expression in 416 clinical samples(208 CRC tissue samples and 208 noncancerous control tissue samples).Clustered regularly interspaced short palindromic repeats(CRISPR)technology was used to evaluate the impact of knocking out KIF20A on CRC cell growth.Molecular docking was applied to analyze NC–KIF20A binding.Finally,RNA sequencing and functional enrichment analysis were performed to explore the mechanism of action of NC in CRC cells.RESULTS Treating HCT116 cells with NC was found to significantly downregulate KIF20A(P<0.05),and the molecular docking analysis revealed high-affinity binding between NC and KIF20A(binding energy=-9.6 kcal/mol).The scRNA-seq,spatial transcriptomics,and mRNA expression profiling results confirmed the significantly high expression of KIF20A in CRC tissues(standardized mean difference=1.33,95%confidence interval:0.885-1.77,summary receiver operating characteristic curve area=0.94).The immunohistochemical analysis of the clinical samples showed high KIF20A expression in the CRC tissues(P<0.05),with significant correlation between the level of expression and gender,tumor size,and tumor grade(P<0.05).Knocking out KIF20A significantly inhibited the growth of various CRC cell lines(CRISPR score<-0.3).The functional enrichment analysis indicated that NC may inhibit CRC by disrupting several biological processes,such as mitotic nuclear division,chromosome segregation,and microtubule binding.CONCLUSION Our results indicate that NC binds to KIF20A with high affinity and downregulates its expression in CRC cells,leading to reduced proliferation.Hence,NC has promise as a therapeutic agent in the treatment of CRC,and targeting KIF20A also has potential as a therapeutic strategy.Further KIF20A knockout studies are needed to confirm the binding specificity and mechanistic roles of NC in CRC.
基金Supported by National Natural Science Foundation of China,No.82260581.
文摘BACKGROUND Hepatocellular carcinoma(HCC)remains a lethal malignancy due to its molecular complexity and chemoresistance.Rac family small GTPase 3(RAC3),a tumorigenic GTPase understudied in HCC,drives recurrence via E2F transcription factor 1(E2F1)-mediated transcriptional activation.This study integrates multiomics and clustered regularly interspaced short palindromic repeats(CRISPR)screening to delineate RAC3’s roles.RAC3 overexpression correlates with advanced HCC and patient age,while its knockout suppresses proliferation.Mechanistically,RAC3 dysregulates cell-cycle checkpoints through E2F1 binding.Pharmacological RAC3 inhibition disrupts tumor growth and synergizes with chemotherapy to overcome resistance.AIM To explore RAC3’s expression,clinical links,and HCC mechanisms via multiomics and functional genomics.METHODS Multiomic integration of The Cancer Genome Atlas(TCGA),Gene Expression Omnibus,and Genotype-Tissue Expression datasets was performed to analyze RAC3 mRNA expression.Immunohistochemistry quantified RAC3 protein in 108 HCC/adjacent tissue pairs.Kaplan–Meier/Cox regression assessed prognostic significance using TCGA data.CRISPR screening validated RAC3’s necessity for HCC proliferation.Functional enrichment identified associated pathways;hTFtarget/JASPAR predicted transcription factors,validated via chromatin immunoprecipitation sequencing(ChIP-seq).RESULTS RAC3 exhibited significant mRNA and protein overexpression in HCC tissues,which was correlated with advanced tumor stages and reduced overall survival rates(hazard ratio=1.82,95%CI:1.31–2.53).Genetic ablation of RAC3 suppressed HCC cell proliferation across 16 cell lines.Pathway analysis revealed RAC3’s predominant involvement in cell-cycle regulation,DNA replication,and nucleocytoplasmic transport.Mechanistic investigations identified E2F1 as a pivotal upstream transcriptional regulator,and ChIP-seq analysis validated its direct binding to the RAC3 promoter region.These findings suggest that RAC3 drives HCC progression through E2F1-mediated cell-cycle dysregulation.CONCLUSION This study identified RAC3 as a key HCC oncogenic driver;its overexpression links to poor prognosis/resistance.Targeting the RAC3/E2F1 axis offers a new therapy,which highlights RAC3 as a biomarker/target.
文摘An H-polygon is a polygon with corners in the set of vertices of a tiling of R~2 by regular hexagons of unit edge.It is known that any H-triangle with exactly k interior H-points can have at most 3k+7 boundary H-points.In this note we prove that any convex H-quadrilateral with exactly k interior H-points can have at most 3k+7 boundary H-points.
文摘The finite volume method was applied to numerically simulate the bottom pressure field induced by regular waves,vehicles in calm water and vehicles in regular waves.The solution of Navier-Stokes(N-S)equations in the vicinity of numerical wave tank's boundary was forced towards the wave theoretical solution by incorporating momentum source terms,thereby reducing adverse effects such as wave reflection.Simulations utilizing laminar flow,turbulent flow,and ideal fluid models were all found capable of effectively capturing the waveform and bottom pressure of regular waves,agreeing well with experimental data.In predicting the bottom pressure field of the submerged vehicle,turbulent simulations considering fluid viscosity and boundary layer development provided more accurate predictions for the stern region than inviscid simulations.Due to sphere's diffractive effect,the sphere's bottom pressure field in waves is not a linear superposition of the wave's and the sphere's bottom pressure field.However,a slender submerged vehicle exhibits a weaker diffractive effect on waves,thus the submerged vehicle's bottom pressure field in waves can be approximated as a linear superposition of the wave's and the submerged vehicle's bottom pressure field,which simplifies computation and analysis.
基金supported by the National Natural Science Foundation of China(11931009,12271495,11971450,and 12071449)Anhui Initiative in Quantum Information Technologies(AHY150200)the Project of Stable Support for Youth Team in Basic Research Field,Chinese Academy of Sciences(YSBR-001).
文摘On a compact Riemann surface with finite punctures P_(1),…P_(k),we define toric curves as multivalued,totallyunramified holomorphic maps to P^(n)with monodromy in a maximal torus of PSU(n+1).Toric solutions to SU(n+1)Todasystems on X\{P_(1);…;P_(k)}are recognized by the associated toric curves in.We introduce character n-ensembles as-tuples of meromorphic one-forms with simple poles and purely imaginary periods,generating toric curves on minus finitelymany points.On X,we establish a correspondence between character-ensembles and toric solutions to the SU(n+1)system with finitely many cone singularities.Our approach not only broadens seminal solutions with two conesingularities on the Riemann sphere,as classified by Jost-Wang(Int.Math.Res.Not.,2002,(6):277-290)andLin-Wei-Ye(Invent.Math.,2012,190(1):169-207),but also advances beyond the limits of Lin-Yang-Zhong’s existencetheorems(J.Differential Geom.,2020,114(2):337-391)by introducing a new solution class.
基金Supported by 2024 Red Imported Fire Ant Prevention and Control Project of Huizhou City.
文摘The prevalence of Solenopsis invicta is associated with habitat diversity,with a preference for various habitats ranked in the following order:ponds,wastelands,green belts,roads,parks,farmlands,and villages.Consequently,management strategies should be tailored to each habitat.There are two occurrence peaks of S.invicta throughout the year,specifically from March to April and from September to October.It is essential to emphasize prevention and control measures during the active periods of S.invicta.To enhance the effectiveness of these measures,supplementary control should be implemented within 10-15 d following the initial prevention and control efforts.Furthermore,the efficacy of prevention and control is notably higher on the second to third day immediately following rainfall.This study aimed to evaluate the efficacy of various agents in controlling S.invicta.A comparative analysis was conducted on the efficacy of three different brands of agents against S.invicta.The findings indicated that a concentration of 0.08%-0.10%indoxacarb exhibited a comparable extermination effect on S.invicta worker ants.However,agents with larger particle sizes demonstrated a reduced efficacy in exterminating S.invicta nests.
基金Supported by the National Natural Science Foundation of China(12001327,12071057)。
文摘Toric patch is a kind of rational multisided patch,which is associated with a finite integer lattice points set A.A set of weights is defined which depend on a parameter according to regular decomposition of A.When all weights of the patch tend to infinity,we obtain the limiting form of toric patch which is called its regular control surface.The diferent weights may induce the diferent regular control surfaces of the same toric patch.It prompts us to consider that how many regular control surfaces of a toric patch.In this paper,we study the regular decompositions of A by using integer programming method firstly,and then provide the relationship between all regular decompositions of A and corresponding state polytope.Moreover,we present that the number of regular control surfaces of a toric patch associated with A is equal to the number of regular decompositions of A.An algorithm to calculate the number of regular control surfaces of toric patch is provided.The algorithm also presents a method to construct all of the regular control surfaces of a toric patch.At last,the application of proposed result in shape deformation is demonstrated by several examples.
基金funded by the National Key R&D Program of China(Grant no.2018YFA0702504)the Sinopec research project(P22162).
文摘Absorption compensation is a process involving the exponential amplification of reflection amplitudes.This process amplifies the seismic signal and noise,thereby substantially reducing the signal-tonoise ratio of seismic data.Therefore,this paper proposes a multichannel inversion absorption compensation method based on structure tensor regularization.First,the structure tensor is utilized to extract the spatial inclination of seismic signals,and the spatial prediction filter is designed along the inclination direction.The spatial prediction filter is then introduced into the regularization condition of multichannel inversion absorption compensation,and the absorption compensation is realized under the framework of multichannel inversion theory.The spatial predictability of seismic signals is also introduced into the objective function of absorption compensation inversion.Thus,the inversion system can effectively suppress the noise amplification effect during absorption compensation and improve the recovery accuracy of high-frequency signals.Synthetic and field data tests are conducted to demonstrate the accuracy and effectiveness of the proposed method.
基金supported by the National Natural Science Foundation of China(No.41804141)。
文摘Energy resolution calibration is crucial for gamma-ray spectral analysis,as measured using a scintillation detector.A locally constrained regularization method was proposed to determine the resolution calibration parameters.First,a Monte Carlo simulation model consistent with an actual measurement system was constructed to obtain the energy deposition distribution in the scintillation crystal.Subsequently,the regularization objective function is established based on weighted least squares and additional constraints.Additional constraints were designed using a special weighting scheme based on the incident gamma-ray energies.Subsequently,an intelligent algorithm was introduced to search for the optimal resolution calibration parameters by minimizing the objective function.The most appropriate regularization parameter was determined through mathematical experiments.When the regularization parameter was 30,the calibrated results exhibited the minimum RMSE.Simulations and test pit experiments were conducted to verify the performance of the proposed method.The simulation results demonstrate that the proposed algorithm can determine resolution calibration parameters more accurately than the traditional weighted least squares,and the test pit experimental results show that the R-squares between the calibrated and measured spectra are larger than 0.99.The accurate resolution calibration parameters determined by the proposed method lay the foundation for gamma-ray spectral processing and simulation benchmarking.
