Objective:Verrucous epidermal nevus(VEN),seborrheic keratosis(SK),verruca plana(VP),verruca vulgaris(VV),and nevus sebaceous(NS)are common verrucous proliferative skin diseases with similar clinical appearances,often ...Objective:Verrucous epidermal nevus(VEN),seborrheic keratosis(SK),verruca plana(VP),verruca vulgaris(VV),and nevus sebaceous(NS)are common verrucous proliferative skin diseases with similar clinical appearances,often posing diagnostic challenges.Dermoscopy and reflectance confocal microscopy(RCM)can aid in their differentiation,yet their specific features under these tools have not been systematically described.This study aims to summarize and analyze the dermoscopic and RCM features of VEN,SK,VP,VV,and NS.Methods:A total of 121 patients with histopathologically confirmed verrucous proliferative skin diseases were enrolled.Dermoscopy and RCM imaging was used to observe and analyze the microscopic features of these conditions.Results:Under dermoscopy,the 5 diseases displayed distinct characteristics:VEN typically showed gyriform structures;SK was characterized by gyriform structures,comedo-like openings,and milia-like cysts;VP and VV featured dotted vessels and frogspawn-like structures;NS presented as brownish-yellow globules.RCM revealed shared features such as hyperkeratosis and acanthosis across all 5 diseases.Specific features included gyriform structures and elongated rete ridges in VEN;pseudocysts and gyriform structures in SK;evenly distributed ring-like structures in VP;vacuolated cells and papillomatous proliferation in VV;and frogspawn-like structures in NS.Conclusion:These 5 verrucous proliferative skin conditions exhibit distinguishable features under both dermoscopy and RCM.The combination of these 2 noninvasive imaging modalities holds significant clinical value for the differential diagnosis of verrucous proliferative skin diseases.展开更多
We report on two strategies to design and implement the galvanometer-based laser-scanning mechanisms for the realization of reflectance confocal microscopy(RCM) and stimulated Raman scattering(SRS) microscopy systems....We report on two strategies to design and implement the galvanometer-based laser-scanning mechanisms for the realization of reflectance confocal microscopy(RCM) and stimulated Raman scattering(SRS) microscopy systems. The RCM system uses a resonant galvanometer scanner driven by a home-built field-programmable gate array circuit with a novel dual-trigger mode and a home-built high-speed data acquisition card. The SRS system uses linear galvanometers with commercially available modules. We demonstrate video-rate high-resolution imaging at 11 frames per second of in vivo human skin with the RCM system and label-free biomolecular imaging of cancer cells with the SRS system. A comparison of the two strategies for controlling galvanometer scanners provides scientific and technical reference for future design and commercialization of various laser-scanning microscopes using galvanometers.展开更多
Objective:Extramammary Paget’s disease(EMPD)is often misdiagnosed as chronic dermatitis or eczema of the scrotum because of its clinical manifestations,leading to delayed diagnosis and treatment.Reflectance confocal ...Objective:Extramammary Paget’s disease(EMPD)is often misdiagnosed as chronic dermatitis or eczema of the scrotum because of its clinical manifestations,leading to delayed diagnosis and treatment.Reflectance confocal microscopy(RCM)and dermoscopy are widely used in clinical practice,enabling early diagnosis and treatment assessment of skin diseases,particularly which are easily misdiagnosed.This study aimed to summarize the RCM and dermoscopic features of EMPD which may provide evidence for diagnosis and evaluation of treatment effects.Methods:A retrospective analysis was conducted on skin lesions from 34 patients diagnosed with EMPD,confirmed through histopathological examination at the dermatology departments of Chengdu Second People’s Hospital and the Japan-China Friendship Hospital between January 2022 and December 2023.The analysis focused on typical changes of lesions,such as blood vessel patterns and background color under dermoscopy,as well as cellular morphology under RCM.Count data were presented as constituent ratios.Results:Among the included 34 patients,28 were male and 6 were female,and 104 skin lesions were identified.The lesions were classified into 3 types:36 red patches,41 dark red erosive patches,and 27 brown pigmented patches.Dermoscopic examination of EMPD revealed characteristic features,including milky-white globules(90.4%,94/104),irregular shiny white streaks(91.3%,95/104),dotted blood vessels(91.3%,95/104),and glomerular-like vessels(88.5%,92/104).RCM demonstrated epidermal structural disorganization(100.0%,104/104),vacuolated cells in the epidermis(94.2%,98/104),and Paget cells,either scattered or clustered(88.5%,92/104).The red macular lesions predominantly contained isolated vacuolated cells in the epidermis,the dark red erosive lesions exhibited Paget cell nests in the basal layer,and the brown pigmented lesions contained numerous dendritic cells.All the patients underwent wide surgical excision followed by RCM and dermoscopic examinations postoperatively.Conclusion:This study identified characteristic dermoscopic and RCM features in patients with EMPD,providing a foundation for noninvasive auxiliary diagnosis and guiding postoperative follow-up.Additionally,RCM characteristics of different lesion morphologies can help physicians more accurately assess surgical margins,leading to more precise surgical treatment options.展开更多
In accordance with the World Health Organization data,cancer remains at the forefront of fatal diseases.An upward trend in cancer incidence and mortality has been observed globally,emphasizing that efforts in developi...In accordance with the World Health Organization data,cancer remains at the forefront of fatal diseases.An upward trend in cancer incidence and mortality has been observed globally,emphasizing that efforts in developing detection and treatment methods should continue.The diagnostic path typically begins with learning the medical history of a patient;this is followed by basic blood tests and imaging tests to indicate where cancer may be located to schedule a needle biopsy.Prompt initiation of diagnosis is crucial since delayed cancer detection entails higher costs of treatment and hospitalization.Thus,there is a need for novel cancer detection methods such as liquid biopsy,elastography,synthetic biosensors,fluorescence imaging,and reflectance confocal microscopy.Conventional therapeutic methods,although still common in clinical practice,pose many limitations and are unsatisfactory.Nowadays,there is a dynamic advancement of clinical research and the development of more precise and effective methods such as oncolytic virotherapy,exosome-based therapy,nanotechnology,dendritic cells,chimeric antigen receptors,immune checkpoint inhibitors,natural product-based therapy,tumor-treating fields,and photodynamic therapy.The present paper compares available data on conventional and modern methods of cancer detection and therapy to facilitate an understanding of this rapidly advancing field and its future directions.As evidenced,modern methods are not without drawbacks;there is still a need to develop new detection strategies and therapeutic approaches to improve sensitivity,specificity,safety,and efficacy.Nevertheless,an appropriate route has been taken,as confirmed by the approval of some modern methods by the Food and Drug Administration.展开更多
Introduction:Eruptive pseudoangiomatosis(EP)is a rare type of cutaneous exanthema characterized by red angioma-like papules.An association of EP with viral infection has been reported.We herein describe 4 pediatric pa...Introduction:Eruptive pseudoangiomatosis(EP)is a rare type of cutaneous exanthema characterized by red angioma-like papules.An association of EP with viral infection has been reported.We herein describe 4 pediatric patients who showed a new variant of EP-associated rash with atypical clinical features and positivity for parvovirus B19 infection.Case presentation:Pediatric patients showed a new variant of EP-associated rash with atypical clinical features by dermoscopy and reflectance confocal microscopy.The papules were localized to the face(cheeks,forehead,and eyelids).Dermoscopy showed multiple red dots suggesting dilated capillaries surrounded by reddish structureless areas;and in vivo reflectance confocal microscopy(RCM)showed perivascular inflammation around the dermal papillae.However,characteristic perilesional halo was absent.In all patients,serology revealed evidence of active infection by parvovirus B19.Discussion:Few cases of a facial pseudoangiomatous rash that is related to parvovirus B19 infection and lacks the typical anemic halo have been reported in the literature.Conclusion:These cases provide new information on the clinical spectrum of EP and its association with parvovirus.展开更多
基金supported by the Project of Health Committee of Hunan Province(D202304128868),China.
文摘Objective:Verrucous epidermal nevus(VEN),seborrheic keratosis(SK),verruca plana(VP),verruca vulgaris(VV),and nevus sebaceous(NS)are common verrucous proliferative skin diseases with similar clinical appearances,often posing diagnostic challenges.Dermoscopy and reflectance confocal microscopy(RCM)can aid in their differentiation,yet their specific features under these tools have not been systematically described.This study aims to summarize and analyze the dermoscopic and RCM features of VEN,SK,VP,VV,and NS.Methods:A total of 121 patients with histopathologically confirmed verrucous proliferative skin diseases were enrolled.Dermoscopy and RCM imaging was used to observe and analyze the microscopic features of these conditions.Results:Under dermoscopy,the 5 diseases displayed distinct characteristics:VEN typically showed gyriform structures;SK was characterized by gyriform structures,comedo-like openings,and milia-like cysts;VP and VV featured dotted vessels and frogspawn-like structures;NS presented as brownish-yellow globules.RCM revealed shared features such as hyperkeratosis and acanthosis across all 5 diseases.Specific features included gyriform structures and elongated rete ridges in VEN;pseudocysts and gyriform structures in SK;evenly distributed ring-like structures in VP;vacuolated cells and papillomatous proliferation in VV;and frogspawn-like structures in NS.Conclusion:These 5 verrucous proliferative skin conditions exhibit distinguishable features under both dermoscopy and RCM.The combination of these 2 noninvasive imaging modalities holds significant clinical value for the differential diagnosis of verrucous proliferative skin diseases.
基金the China Scholar・ship Council(No.201904910117)Jilin Province Talent Development Fund[2018]853 awarded to F.Wang。
文摘We report on two strategies to design and implement the galvanometer-based laser-scanning mechanisms for the realization of reflectance confocal microscopy(RCM) and stimulated Raman scattering(SRS) microscopy systems. The RCM system uses a resonant galvanometer scanner driven by a home-built field-programmable gate array circuit with a novel dual-trigger mode and a home-built high-speed data acquisition card. The SRS system uses linear galvanometers with commercially available modules. We demonstrate video-rate high-resolution imaging at 11 frames per second of in vivo human skin with the RCM system and label-free biomolecular imaging of cancer cells with the SRS system. A comparison of the two strategies for controlling galvanometer scanners provides scientific and technical reference for future design and commercialization of various laser-scanning microscopes using galvanometers.
基金supported by the National Natural Science Foundation of China Youth Project(No.82103100)the Chengdu Municipal Health Commission Research Project(No.2021201).
文摘Objective:Extramammary Paget’s disease(EMPD)is often misdiagnosed as chronic dermatitis or eczema of the scrotum because of its clinical manifestations,leading to delayed diagnosis and treatment.Reflectance confocal microscopy(RCM)and dermoscopy are widely used in clinical practice,enabling early diagnosis and treatment assessment of skin diseases,particularly which are easily misdiagnosed.This study aimed to summarize the RCM and dermoscopic features of EMPD which may provide evidence for diagnosis and evaluation of treatment effects.Methods:A retrospective analysis was conducted on skin lesions from 34 patients diagnosed with EMPD,confirmed through histopathological examination at the dermatology departments of Chengdu Second People’s Hospital and the Japan-China Friendship Hospital between January 2022 and December 2023.The analysis focused on typical changes of lesions,such as blood vessel patterns and background color under dermoscopy,as well as cellular morphology under RCM.Count data were presented as constituent ratios.Results:Among the included 34 patients,28 were male and 6 were female,and 104 skin lesions were identified.The lesions were classified into 3 types:36 red patches,41 dark red erosive patches,and 27 brown pigmented patches.Dermoscopic examination of EMPD revealed characteristic features,including milky-white globules(90.4%,94/104),irregular shiny white streaks(91.3%,95/104),dotted blood vessels(91.3%,95/104),and glomerular-like vessels(88.5%,92/104).RCM demonstrated epidermal structural disorganization(100.0%,104/104),vacuolated cells in the epidermis(94.2%,98/104),and Paget cells,either scattered or clustered(88.5%,92/104).The red macular lesions predominantly contained isolated vacuolated cells in the epidermis,the dark red erosive lesions exhibited Paget cell nests in the basal layer,and the brown pigmented lesions contained numerous dendritic cells.All the patients underwent wide surgical excision followed by RCM and dermoscopic examinations postoperatively.Conclusion:This study identified characteristic dermoscopic and RCM features in patients with EMPD,providing a foundation for noninvasive auxiliary diagnosis and guiding postoperative follow-up.Additionally,RCM characteristics of different lesion morphologies can help physicians more accurately assess surgical margins,leading to more precise surgical treatment options.
文摘In accordance with the World Health Organization data,cancer remains at the forefront of fatal diseases.An upward trend in cancer incidence and mortality has been observed globally,emphasizing that efforts in developing detection and treatment methods should continue.The diagnostic path typically begins with learning the medical history of a patient;this is followed by basic blood tests and imaging tests to indicate where cancer may be located to schedule a needle biopsy.Prompt initiation of diagnosis is crucial since delayed cancer detection entails higher costs of treatment and hospitalization.Thus,there is a need for novel cancer detection methods such as liquid biopsy,elastography,synthetic biosensors,fluorescence imaging,and reflectance confocal microscopy.Conventional therapeutic methods,although still common in clinical practice,pose many limitations and are unsatisfactory.Nowadays,there is a dynamic advancement of clinical research and the development of more precise and effective methods such as oncolytic virotherapy,exosome-based therapy,nanotechnology,dendritic cells,chimeric antigen receptors,immune checkpoint inhibitors,natural product-based therapy,tumor-treating fields,and photodynamic therapy.The present paper compares available data on conventional and modern methods of cancer detection and therapy to facilitate an understanding of this rapidly advancing field and its future directions.As evidenced,modern methods are not without drawbacks;there is still a need to develop new detection strategies and therapeutic approaches to improve sensitivity,specificity,safety,and efficacy.Nevertheless,an appropriate route has been taken,as confirmed by the approval of some modern methods by the Food and Drug Administration.
文摘Introduction:Eruptive pseudoangiomatosis(EP)is a rare type of cutaneous exanthema characterized by red angioma-like papules.An association of EP with viral infection has been reported.We herein describe 4 pediatric patients who showed a new variant of EP-associated rash with atypical clinical features and positivity for parvovirus B19 infection.Case presentation:Pediatric patients showed a new variant of EP-associated rash with atypical clinical features by dermoscopy and reflectance confocal microscopy.The papules were localized to the face(cheeks,forehead,and eyelids).Dermoscopy showed multiple red dots suggesting dilated capillaries surrounded by reddish structureless areas;and in vivo reflectance confocal microscopy(RCM)showed perivascular inflammation around the dermal papillae.However,characteristic perilesional halo was absent.In all patients,serology revealed evidence of active infection by parvovirus B19.Discussion:Few cases of a facial pseudoangiomatous rash that is related to parvovirus B19 infection and lacks the typical anemic halo have been reported in the literature.Conclusion:These cases provide new information on the clinical spectrum of EP and its association with parvovirus.
