Many design engineers in cross-domain industries have attended training classes of TRIZ to improve their innovative abilities in China. Most of them are successful, but others are not. So the latest target of the trai...Many design engineers in cross-domain industries have attended training classes of TRIZ to improve their innovative abilities in China. Most of them are successful, but others are not. So the latest target of the trainers is to improve the training process used now in industry in China and to make the engineers to understand the basic principles of TRIZ better. Based on the mass-engineer-oriented training model(MEOTM) and mechanical engineers’ design cases, a relationship between managing activities about the opportunities for innovation and the training process is set up. It is shown that the inventive problems come first from opportunity searching for engineers. A training and gate system for evaluation is developed to involve the managing activities of the companies in the training process. Then comparison between the general analogous process and the application of TRIZ is made, which shows the advantages and depth principles of TRIZ for the engineers to apply them confidently. Lastly a new process is formed in which opportunity searching, engineers training, inventive problems identifying and solving,and three redesign paths are connected seamlessly. The research proposes an opportunity-driven redesign path that cooperates the training and opportunity searching, which will be applied in future training classes to make more and more engineers to follow.展开更多
The paper investigates the learner-focused English as a Foreign Langue(EFL) curricular innovation in China Agricultural University(referred to as CAU in the following text) from the perspectives of curricular formulat...The paper investigates the learner-focused English as a Foreign Langue(EFL) curricular innovation in China Agricultural University(referred to as CAU in the following text) from the perspectives of curricular formulation,implementation and evaluation of EFL learning & teaching in the one-year duration from Autumn semester of 2016 to Spring semester of 2017.A discussion of the effects of the curricular restructuring is presented.展开更多
Currently, simultaneously ensuring the machining accuracy and efficiency of thin-walled structures especially high performance parts still remains a challenge. Existing compensating methods are mainly focusing on 3-ai...Currently, simultaneously ensuring the machining accuracy and efficiency of thin-walled structures especially high performance parts still remains a challenge. Existing compensating methods are mainly focusing on 3-aixs machining, which sometimes only take one given point as the compensative point at each given cutter location. This paper presents a redesigned surface based machining strategy for peripheral milling of thin-walled parts. Based on an improved cutting force/heat model and finite element method（FEM） simulation environment, a deflection error prediction model, which takes sequence of cutter contact lines as compensation targets, is established. And an iterative algorithm is presented to determine feasible cutter axis positions. The final redesigned surface is subsequently generated by skinning all discrete cutter axis vectors after compensating by using the proposed algorithm. The proposed machining strategy incorporates the thermo-mechanical coupled effect in deflection prediction, and is also validated with flank milling experiment by using five-axis machine tool. At the same time, the deformation error is detected by using three-coordinate measuring machine. Error prediction values and experimental results indicate that they have a good consistency and the proposed approach is able to significantly reduce the dimension error under the same machining conditions compared with conventional methods. The proposed machining strategy has potential in high-efficiency precision machining of thin-walled parts.展开更多
Human noroviruses(HuNoVs)are major foodborne pathogens that cause nonbacterial acute gastroenteritis worldwide.As the tissue-culture system for HuNoVs is not mature enough for routine detection of the virus,detection ...Human noroviruses(HuNoVs)are major foodborne pathogens that cause nonbacterial acute gastroenteritis worldwide.As the tissue-culture system for HuNoVs is not mature enough for routine detection of the virus,detection is mainly dependent on molecular approaches such as reverse transcription polymerase chain reaction(RT-PCR)and reverse transcription quantitative real-time polymerase chain reaction(RTqPCR).The widely used primers and probes for RT-qPCR were established in the early 2000s.As HuNoVs are highly variant viruses,viral genome mutations result in previously designed primers and/or probes that were perfectly matched working less efficiently over time.In this study,a new duplex RT-qPCR(ND-RT-qPCR)was designed for the detection of genogroup Ⅰ(GⅠ)and genogroup Ⅱ(GⅡ)HuNoVs based on an analysis of viral sequences added in the database after 2010.Using long transcribed viral RNAs,the results demonstrate that the sensitivity of ND-RT-qPCR is as low as one genomic copy for both GⅠ and GⅡ HuNoVs.The performance of ND-RT-qPCR was further evaluated by a comparison with the commonly used Kageyama primer/probe sets for RT-qPCR(Kageyama RT-qPCR)for 23 HuNoV-positive clinical samples.All five GⅠ samples were registered as positive by ND-RT-qPCR,whereas only two samples were registered as positive by Kageyama RT-qPCR.All 18 GⅡ samples were registered as positive by ND-RT-qPCR,while 17 samples were registered as positive by Kageyama RT-qPCR.The sensitivity reflected by the quantification cycle(Cq)value was lower in ND-RT-qPCR than in Kageyama RT-qPCR.Our data suggest that ND-RT-qPCR could be a good fit for the detection of current strains of HuNoVs.展开更多
The rapid development of urbanization and rapid growth of urban population have brought about various urban problems, and the development of cities to the ground and underground has become an inevitable choice. Underg...The rapid development of urbanization and rapid growth of urban population have brought about various urban problems, and the development of cities to the ground and underground has become an inevitable choice. Underground street is a kind of underground space types, and it is also an important urban recreational resources and tourism resources. The evaluation of underground street users, especially the residents and tourists, has important reference value for the recreational resources’ redevelopment of underground street. Firstly, this paper reviews the relevant research literature on underground street and Post-Occupancy Evaluation theory, then chooses Diyi Underground Street, Chengdu, China, as the research object, and conducts a questionnaire survey and in-depth interviews on residents’ shopping intention from five dimensions of underground street environment, decoration, commercial form, public facilities and other aspects. Finally, the survey data are analyzed quantitatively. The results show that some influence factors such as air quality, daylighting, decoration style etc. greatly affect the willingness of residents to enter the street. Therefore, residents’ recreational experience should be emphasized during the underground street re-planning according to the above factors.展开更多
Horizontal press as an important part of hydro-forming machine is used to output the horizontal force to keep the high internal pressure during tube hydro-forming. However,the horizontal press frame is usually mounted...Horizontal press as an important part of hydro-forming machine is used to output the horizontal force to keep the high internal pressure during tube hydro-forming. However,the horizontal press frame is usually mounted on the press bed and not pre-stressed. Meanwhile it will be subjected to the reaction force caused by liquid pressure. Stresses are concentrated severely on the assemble region due to deformation,and total fatigue life will decrease. In order to predict the total fatigue life of the frame,the simulations are used firstly to determine to stress concentration region,and then strain gauge measurements are carried out under different loads. Next,the methods of statistical probability are conducted to calculate the fatigue life based on long-term load history. Finally a structure with the considerable longer fatigue life is redesigned.展开更多
In improving the competitiveness of business organi sa tion in the 21st century, minimising cost and increasing productivity are no lon ger factors that could promise the success. The changes in customer trends whic h...In improving the competitiveness of business organi sa tion in the 21st century, minimising cost and increasing productivity are no lon ger factors that could promise the success. The changes in customer trends whic h focusing more on product or service customising, high quality and short delive ry times are additional crucial factors that organisation should be aware of. T hese factors have direct relations on how the management could utilise the capab ility of its supply chain management (SCM). The importance of SCM in organisati on specifically to manufacturer is to play a vital role in managing the flow of material and information along the chain from suppliers to customers. In a trad itional way, SCM is mainly manage by the production department in organisation b ut with the advancement in information technology, resulted the changes in worki ng environment. One of the ways that could be implemented in decision making p rocess in order to improve the effectiveness and efficiency of the decision is t hrough collaborative environment. Due to this situation, there is a need to rede sign the existing SCM in order to optimise its capability and functionality in t he way to ensure the organisation competitiveness is sustainable. A minor or ma jor alignment to SCM business processes should be done in order to streamline th e flow of information, which could also affect the flow of materials. Factors s uch as SCM operational, structural and even its managerial are among the issues that are critical in redesigning its business process. Each of these factors has its own attributes such as lead-times, complexity, frequency and organisationa l setting that could lead in improving organisational competitiveness. In additi on, by identifying these factors, it would help the management to plan and desig n the collaborative SCM that would effectively correspond to the changes in busi ness and customer trends. The intention of the paper is to promote a list of fa ctors and attributes, which are critical in redesigning an existing SCM in order to shift its environment to become collaborative SCM. By utilising these facto rs and attributes, a model for redesigning SCM into collaborative environment is currently developed by the author and will be used in the next stage of his res earch.展开更多
The particular characteristics of Rapid Prototyping technologies, both in terms of constrains and opportunities, often require the reconfiguration of the product model to obtain the best compliance with the product fu...The particular characteristics of Rapid Prototyping technologies, both in terms of constrains and opportunities, often require the reconfiguration of the product model to obtain the best compliance with the product functionalities and performances. Within this field of research, a knowledge-based tool named Design GuideLines Collaborative Framework (DGLs-CF) was developed to support both the designers defining the product consistently with the manufacturing technologies and the manufacturers defining the building setup consistently with the product requirements. Present work is focused on enhancing the DGLs-CF knowledge base and on updating the DGLs-CF knowledge management by using the information gathered on some RP technologies. The added-value of this research is represented by an improvement in the Redesign/Reconfig- uration Package, the final result of the DGLs-CF adoption. This is a list of actions to be performed on the product model and on the process parameters to avoid the limitations of the technology and to exploit at best its opportunities.展开更多
<em>Kaba</em> is considered to be a kind of traditional apparel worn by the women in Ghana. Though, it’s worn to accentuate female modesty, chastity and elegance among the women folks. The modern day woma...<em>Kaba</em> is considered to be a kind of traditional apparel worn by the women in Ghana. Though, it’s worn to accentuate female modesty, chastity and elegance among the women folks. The modern day woman faces a lot of physical and emotional challenges when it comes to breastfeeding. There is a need to redesign and improve upon the <em>kaba</em> apparel for breastfeeding purposes. This study, therefore, sought to redesign functional <em>kaba</em> apparel for breastfeeding mothers. Based on the apparel design framework of Lamb and Kallal, design requirements were identified from breastfeeding mothers and incorporated into the designed apparel. Five prototype designs were made and tested by three selected mothers towards effective breastfeeding. Findings revealed that the prototype apparels made of fasteners and openings in the horizontal, diagonal and vertical directions with zippers and buttons were said to be suitable, comfortable to use and hence acceptable for implementation. This added functional part of the apparel boosted the comfort and confidence of the mother without compromising on their expressive values.展开更多
At present, whether internally or abroad, whether in the theory or business, there is a lack of a unified understanding about the basic concepts of structural system and theoretical category of enterprise business mod...At present, whether internally or abroad, whether in the theory or business, there is a lack of a unified understanding about the basic concepts of structural system and theoretical category of enterprise business model, and its theoretical analysis framework has not been built up, it is the exploratory phase of the model research in the mass. This study aims to analyze the formation mechanism, action mechanism, and evolution mechanism about the business model from the perspective of structure, and designs the enterprise business model architecture and forms a system, besides, explores approaches and strategies about enterprise carrying out business model innovation, and does beneficial bedding in order to use and improve the theoretical system in the process of the enterprise management practice. The significance of this study is to provide analytical methods and tools for the business model questions in the enterprise management practice. This paper firstly defined the connotation of enterprise business model and summarized the seven characteristics of the corporate business model. On the basis of outlining the enterprise business model design theory, the structural design of the corporate business model is mainly discussed. This paper designed the structure of the business model on modular perspective, proposed four constituent units and nine elements of enterprise business model structure system. Through the design of the enterprise business model structure and analysis of the elements that constitute structure, provide the basis for the transformation of innovative enterprise business model, or both enterprise business models, avoid the lack of elements of the enterprise business model structure and lead to business failure. Finally, the paper concludes with the five core strategies for the implementation of the enterprise business model design.展开更多
Housing is dynamic. It changes with time. A sample of 60 houses in the Shagari Low Cost housing scheme in Kanra Namoda was studied in order to find out the characteristics of changes made by the users. Physical observ...Housing is dynamic. It changes with time. A sample of 60 houses in the Shagari Low Cost housing scheme in Kanra Namoda was studied in order to find out the characteristics of changes made by the users. Physical observations and questionnaires were used as the major instruments of data collection. The results show that 91% of occupants have made various changes to the original designs without the help of professional designers, and some of the changes have completely transformed the outlook of the houses in locational planning, materials and form. This implies that a post architectural design phase exists, suggesting that the design process can be divided into two phases; the initial design by the architect and the subsequent redesign by the user. The paper presents preliminary findings on the general characteristics of user redesign and concludes that a clear process can be established. The architectural design process can, therefore, be expanded to include the user redesign process in order to reflect the entire lifespan of the building.展开更多
Redesign of motorized and non-motorized transport in cities and sustainable mobility possibilities and utilization of already occupied areas for motorized and non-motorized transport in cities. Reserved area for publi...Redesign of motorized and non-motorized transport in cities and sustainable mobility possibilities and utilization of already occupied areas for motorized and non-motorized transport in cities. Reserved area for public transport may be the redesign much more to take advantage of without taking up new space in cities. Redesigned solutions shown in the work of public mass transport and the redesign of non-motorized transport, bicycle and pedestrian paths point to the improved use and safety of movement of passengers, cyclists and pedestrians. This paper presents five redesigned concept designs as improving existing forms of transport and movement of cyclists and pedestrians in cities. Redesigned conceptual designs of motorized and non-motorized transport in cities should serve as ideas for the growing problems of urban development in the segment of insufficient surface for pedestrians and cyclists, as well as sustainable mobility transport people in the city.展开更多
Biotechnological strategies for plastic depolymerization and recycling have emerged as transformative approaches to combat the global plastic pollution crisis,aligning with the principles of a sustainable and circular...Biotechnological strategies for plastic depolymerization and recycling have emerged as transformative approaches to combat the global plastic pollution crisis,aligning with the principles of a sustainable and circular economy.Despite advances in engineering PET hydrolases,the degradation process is frequently compromised by product inhibition and the heterogeneity of final products,thereby obstructing subsequent PET recondensation and impeding the synthesis of high-value derivatives.In this work,we utilized previously devised computational strategies to redesign a thermostable DuraMHETase,achieving an apparent melting temperature of 72℃ in complex with MHET and a 6-fold higher in total turnover number(TTN)toward MHET than the wild-type enzyme at 60℃.The fused enzyme system composed of DuraMHETase and TurboPETase demonstrated higher efficiency than other PET hydrolases and the separated dual enzyme systems.Furthermore,we identified both exo-and endo-PETase activities in DuraMHETase,whereas the endo-activity was previously unobserved at ambient temperatures.These results expand the functional scope of MHETase beyond mere intermediate hydrolysis,and may provide guidance for the development of more synergistic approaches to plastic biodepolymerization and recycling.展开更多
Plant cell walls are a critical site where plants and pathogens continuously struggle for physiological domi-nance.Here we show that dynamic remodeling of pectin methylesterification of plant cell walls is a compo-nen...Plant cell walls are a critical site where plants and pathogens continuously struggle for physiological domi-nance.Here we show that dynamic remodeling of pectin methylesterification of plant cell walls is a compo-nent of the physiological and co-evolutionary struggles between hosts and pathogens.A pectin methyles-terase(PsPME1)secreted by Phytophthora sojae decreases the degree of pectin methylesterification,thus synergizing with an endo-polygalacturonase(PsPG1)to weaken plant cell walls.To counter PsPME1-mediated susceptibility,a plant-derived pectin methylesterase inhibitor protein,GmPMl1,protects pectin to maintain a high methylesterification status.GmPMl1 protects plant cell walls from enzymatic degrada-tion by inhibiting both soybean and P.sojae pectin methylesterases during infection.However,constitutive expression of GmPMl1 disrupted the trade-off between host growth and defense responses.We therefore used AlphaFold structure tools to design a modified form of GmPMI1(GmPMI1R)that specifically targets and inhibits pectin methylesterases secreted from pathogens but notfrom plants.Transient expression of GmPMi1R enhanced plant resistance to oomycete and fungal pathogens.In summary,our work highlights the biochemical modification of the cell wall as an important focal point in the physiological and co-evolutionary conflict between hosts and microbes,providing an important proof of concept that Al-driven structure-based tools can accelerate the development of new strategies for plant protection.展开更多
基金Supported by National Natural Science Foundation of China(Grant Nos.51675159,51305123)
文摘Many design engineers in cross-domain industries have attended training classes of TRIZ to improve their innovative abilities in China. Most of them are successful, but others are not. So the latest target of the trainers is to improve the training process used now in industry in China and to make the engineers to understand the basic principles of TRIZ better. Based on the mass-engineer-oriented training model(MEOTM) and mechanical engineers’ design cases, a relationship between managing activities about the opportunities for innovation and the training process is set up. It is shown that the inventive problems come first from opportunity searching for engineers. A training and gate system for evaluation is developed to involve the managing activities of the companies in the training process. Then comparison between the general analogous process and the application of TRIZ is made, which shows the advantages and depth principles of TRIZ for the engineers to apply them confidently. Lastly a new process is formed in which opportunity searching, engineers training, inventive problems identifying and solving,and three redesign paths are connected seamlessly. The research proposes an opportunity-driven redesign path that cooperates the training and opportunity searching, which will be applied in future training classes to make more and more engineers to follow.
文摘The paper investigates the learner-focused English as a Foreign Langue(EFL) curricular innovation in China Agricultural University(referred to as CAU in the following text) from the perspectives of curricular formulation,implementation and evaluation of EFL learning & teaching in the one-year duration from Autumn semester of 2016 to Spring semester of 2017.A discussion of the effects of the curricular restructuring is presented.
基金supported by Key Program of National Natural Science Foundation of China (Grant No. 50835001) General Program of National Natural Science Foundation of China (Grant No. 50775023)Program for New Century Excellent Talents of Ministry of Education of China (Grant No. NCET-08-081)
文摘Currently, simultaneously ensuring the machining accuracy and efficiency of thin-walled structures especially high performance parts still remains a challenge. Existing compensating methods are mainly focusing on 3-aixs machining, which sometimes only take one given point as the compensative point at each given cutter location. This paper presents a redesigned surface based machining strategy for peripheral milling of thin-walled parts. Based on an improved cutting force/heat model and finite element method（FEM） simulation environment, a deflection error prediction model, which takes sequence of cutter contact lines as compensation targets, is established. And an iterative algorithm is presented to determine feasible cutter axis positions. The final redesigned surface is subsequently generated by skinning all discrete cutter axis vectors after compensating by using the proposed algorithm. The proposed machining strategy incorporates the thermo-mechanical coupled effect in deflection prediction, and is also validated with flank milling experiment by using five-axis machine tool. At the same time, the deformation error is detected by using three-coordinate measuring machine. Error prediction values and experimental results indicate that they have a good consistency and the proposed approach is able to significantly reduce the dimension error under the same machining conditions compared with conventional methods. The proposed machining strategy has potential in high-efficiency precision machining of thin-walled parts.
基金supported by the Ministry of Science and Technology of China(2017YFC1601200)the National Natural Science Foundation of China(31772078)the Agri-X Interdisciplinary Fund of Shanghai Jiao Tong University(2017).
文摘Human noroviruses(HuNoVs)are major foodborne pathogens that cause nonbacterial acute gastroenteritis worldwide.As the tissue-culture system for HuNoVs is not mature enough for routine detection of the virus,detection is mainly dependent on molecular approaches such as reverse transcription polymerase chain reaction(RT-PCR)and reverse transcription quantitative real-time polymerase chain reaction(RTqPCR).The widely used primers and probes for RT-qPCR were established in the early 2000s.As HuNoVs are highly variant viruses,viral genome mutations result in previously designed primers and/or probes that were perfectly matched working less efficiently over time.In this study,a new duplex RT-qPCR(ND-RT-qPCR)was designed for the detection of genogroup Ⅰ(GⅠ)and genogroup Ⅱ(GⅡ)HuNoVs based on an analysis of viral sequences added in the database after 2010.Using long transcribed viral RNAs,the results demonstrate that the sensitivity of ND-RT-qPCR is as low as one genomic copy for both GⅠ and GⅡ HuNoVs.The performance of ND-RT-qPCR was further evaluated by a comparison with the commonly used Kageyama primer/probe sets for RT-qPCR(Kageyama RT-qPCR)for 23 HuNoV-positive clinical samples.All five GⅠ samples were registered as positive by ND-RT-qPCR,whereas only two samples were registered as positive by Kageyama RT-qPCR.All 18 GⅡ samples were registered as positive by ND-RT-qPCR,while 17 samples were registered as positive by Kageyama RT-qPCR.The sensitivity reflected by the quantification cycle(Cq)value was lower in ND-RT-qPCR than in Kageyama RT-qPCR.Our data suggest that ND-RT-qPCR could be a good fit for the detection of current strains of HuNoVs.
文摘The rapid development of urbanization and rapid growth of urban population have brought about various urban problems, and the development of cities to the ground and underground has become an inevitable choice. Underground street is a kind of underground space types, and it is also an important urban recreational resources and tourism resources. The evaluation of underground street users, especially the residents and tourists, has important reference value for the recreational resources’ redevelopment of underground street. Firstly, this paper reviews the relevant research literature on underground street and Post-Occupancy Evaluation theory, then chooses Diyi Underground Street, Chengdu, China, as the research object, and conducts a questionnaire survey and in-depth interviews on residents’ shopping intention from five dimensions of underground street environment, decoration, commercial form, public facilities and other aspects. Finally, the survey data are analyzed quantitatively. The results show that some influence factors such as air quality, daylighting, decoration style etc. greatly affect the willingness of residents to enter the street. Therefore, residents’ recreational experience should be emphasized during the underground street re-planning according to the above factors.
基金Sponsored by the High-End CNC Machine Tools and Basic Manufacturing Equipment Technology Major Project(Grant No.2011ZX04001-011)
文摘Horizontal press as an important part of hydro-forming machine is used to output the horizontal force to keep the high internal pressure during tube hydro-forming. However,the horizontal press frame is usually mounted on the press bed and not pre-stressed. Meanwhile it will be subjected to the reaction force caused by liquid pressure. Stresses are concentrated severely on the assemble region due to deformation,and total fatigue life will decrease. In order to predict the total fatigue life of the frame,the simulations are used firstly to determine to stress concentration region,and then strain gauge measurements are carried out under different loads. Next,the methods of statistical probability are conducted to calculate the fatigue life based on long-term load history. Finally a structure with the considerable longer fatigue life is redesigned.
文摘In improving the competitiveness of business organi sa tion in the 21st century, minimising cost and increasing productivity are no lon ger factors that could promise the success. The changes in customer trends whic h focusing more on product or service customising, high quality and short delive ry times are additional crucial factors that organisation should be aware of. T hese factors have direct relations on how the management could utilise the capab ility of its supply chain management (SCM). The importance of SCM in organisati on specifically to manufacturer is to play a vital role in managing the flow of material and information along the chain from suppliers to customers. In a trad itional way, SCM is mainly manage by the production department in organisation b ut with the advancement in information technology, resulted the changes in worki ng environment. One of the ways that could be implemented in decision making p rocess in order to improve the effectiveness and efficiency of the decision is t hrough collaborative environment. Due to this situation, there is a need to rede sign the existing SCM in order to optimise its capability and functionality in t he way to ensure the organisation competitiveness is sustainable. A minor or ma jor alignment to SCM business processes should be done in order to streamline th e flow of information, which could also affect the flow of materials. Factors s uch as SCM operational, structural and even its managerial are among the issues that are critical in redesigning its business process. Each of these factors has its own attributes such as lead-times, complexity, frequency and organisationa l setting that could lead in improving organisational competitiveness. In additi on, by identifying these factors, it would help the management to plan and desig n the collaborative SCM that would effectively correspond to the changes in busi ness and customer trends. The intention of the paper is to promote a list of fa ctors and attributes, which are critical in redesigning an existing SCM in order to shift its environment to become collaborative SCM. By utilising these facto rs and attributes, a model for redesigning SCM into collaborative environment is currently developed by the author and will be used in the next stage of his res earch.
文摘The particular characteristics of Rapid Prototyping technologies, both in terms of constrains and opportunities, often require the reconfiguration of the product model to obtain the best compliance with the product functionalities and performances. Within this field of research, a knowledge-based tool named Design GuideLines Collaborative Framework (DGLs-CF) was developed to support both the designers defining the product consistently with the manufacturing technologies and the manufacturers defining the building setup consistently with the product requirements. Present work is focused on enhancing the DGLs-CF knowledge base and on updating the DGLs-CF knowledge management by using the information gathered on some RP technologies. The added-value of this research is represented by an improvement in the Redesign/Reconfig- uration Package, the final result of the DGLs-CF adoption. This is a list of actions to be performed on the product model and on the process parameters to avoid the limitations of the technology and to exploit at best its opportunities.
文摘<em>Kaba</em> is considered to be a kind of traditional apparel worn by the women in Ghana. Though, it’s worn to accentuate female modesty, chastity and elegance among the women folks. The modern day woman faces a lot of physical and emotional challenges when it comes to breastfeeding. There is a need to redesign and improve upon the <em>kaba</em> apparel for breastfeeding purposes. This study, therefore, sought to redesign functional <em>kaba</em> apparel for breastfeeding mothers. Based on the apparel design framework of Lamb and Kallal, design requirements were identified from breastfeeding mothers and incorporated into the designed apparel. Five prototype designs were made and tested by three selected mothers towards effective breastfeeding. Findings revealed that the prototype apparels made of fasteners and openings in the horizontal, diagonal and vertical directions with zippers and buttons were said to be suitable, comfortable to use and hence acceptable for implementation. This added functional part of the apparel boosted the comfort and confidence of the mother without compromising on their expressive values.
文摘At present, whether internally or abroad, whether in the theory or business, there is a lack of a unified understanding about the basic concepts of structural system and theoretical category of enterprise business model, and its theoretical analysis framework has not been built up, it is the exploratory phase of the model research in the mass. This study aims to analyze the formation mechanism, action mechanism, and evolution mechanism about the business model from the perspective of structure, and designs the enterprise business model architecture and forms a system, besides, explores approaches and strategies about enterprise carrying out business model innovation, and does beneficial bedding in order to use and improve the theoretical system in the process of the enterprise management practice. The significance of this study is to provide analytical methods and tools for the business model questions in the enterprise management practice. This paper firstly defined the connotation of enterprise business model and summarized the seven characteristics of the corporate business model. On the basis of outlining the enterprise business model design theory, the structural design of the corporate business model is mainly discussed. This paper designed the structure of the business model on modular perspective, proposed four constituent units and nine elements of enterprise business model structure system. Through the design of the enterprise business model structure and analysis of the elements that constitute structure, provide the basis for the transformation of innovative enterprise business model, or both enterprise business models, avoid the lack of elements of the enterprise business model structure and lead to business failure. Finally, the paper concludes with the five core strategies for the implementation of the enterprise business model design.
文摘Housing is dynamic. It changes with time. A sample of 60 houses in the Shagari Low Cost housing scheme in Kanra Namoda was studied in order to find out the characteristics of changes made by the users. Physical observations and questionnaires were used as the major instruments of data collection. The results show that 91% of occupants have made various changes to the original designs without the help of professional designers, and some of the changes have completely transformed the outlook of the houses in locational planning, materials and form. This implies that a post architectural design phase exists, suggesting that the design process can be divided into two phases; the initial design by the architect and the subsequent redesign by the user. The paper presents preliminary findings on the general characteristics of user redesign and concludes that a clear process can be established. The architectural design process can, therefore, be expanded to include the user redesign process in order to reflect the entire lifespan of the building.
文摘Redesign of motorized and non-motorized transport in cities and sustainable mobility possibilities and utilization of already occupied areas for motorized and non-motorized transport in cities. Reserved area for public transport may be the redesign much more to take advantage of without taking up new space in cities. Redesigned solutions shown in the work of public mass transport and the redesign of non-motorized transport, bicycle and pedestrian paths point to the improved use and safety of movement of passengers, cyclists and pedestrians. This paper presents five redesigned concept designs as improving existing forms of transport and movement of cyclists and pedestrians in cities. Redesigned conceptual designs of motorized and non-motorized transport in cities should serve as ideas for the growing problems of urban development in the segment of insufficient surface for pedestrians and cyclists, as well as sustainable mobility transport people in the city.
文摘Biotechnological strategies for plastic depolymerization and recycling have emerged as transformative approaches to combat the global plastic pollution crisis,aligning with the principles of a sustainable and circular economy.Despite advances in engineering PET hydrolases,the degradation process is frequently compromised by product inhibition and the heterogeneity of final products,thereby obstructing subsequent PET recondensation and impeding the synthesis of high-value derivatives.In this work,we utilized previously devised computational strategies to redesign a thermostable DuraMHETase,achieving an apparent melting temperature of 72℃ in complex with MHET and a 6-fold higher in total turnover number(TTN)toward MHET than the wild-type enzyme at 60℃.The fused enzyme system composed of DuraMHETase and TurboPETase demonstrated higher efficiency than other PET hydrolases and the separated dual enzyme systems.Furthermore,we identified both exo-and endo-PETase activities in DuraMHETase,whereas the endo-activity was previously unobserved at ambient temperatures.These results expand the functional scope of MHETase beyond mere intermediate hydrolysis,and may provide guidance for the development of more synergistic approaches to plastic biodepolymerization and recycling.
基金supported bythe National Key Research and Development Program of China(2022YFF1001500)the National Natural Science Foundation of China(32102172)and(31721004)+1 种基金the China National Postdoctoral Program for Innovative Talents(BX2021130)the China Postdoctoral Science Foundation(2021M700074).
文摘Plant cell walls are a critical site where plants and pathogens continuously struggle for physiological domi-nance.Here we show that dynamic remodeling of pectin methylesterification of plant cell walls is a compo-nent of the physiological and co-evolutionary struggles between hosts and pathogens.A pectin methyles-terase(PsPME1)secreted by Phytophthora sojae decreases the degree of pectin methylesterification,thus synergizing with an endo-polygalacturonase(PsPG1)to weaken plant cell walls.To counter PsPME1-mediated susceptibility,a plant-derived pectin methylesterase inhibitor protein,GmPMl1,protects pectin to maintain a high methylesterification status.GmPMl1 protects plant cell walls from enzymatic degrada-tion by inhibiting both soybean and P.sojae pectin methylesterases during infection.However,constitutive expression of GmPMl1 disrupted the trade-off between host growth and defense responses.We therefore used AlphaFold structure tools to design a modified form of GmPMI1(GmPMI1R)that specifically targets and inhibits pectin methylesterases secreted from pathogens but notfrom plants.Transient expression of GmPMi1R enhanced plant resistance to oomycete and fungal pathogens.In summary,our work highlights the biochemical modification of the cell wall as an important focal point in the physiological and co-evolutionary conflict between hosts and microbes,providing an important proof of concept that Al-driven structure-based tools can accelerate the development of new strategies for plant protection.
