目的:探究花生红衣原花青素(peanut red proanthocyanidins,PRP)对氧化低密度脂蛋白(ox-LDL)诱导的血管平滑肌细胞(Vascular Smooth Muscle Cells,VSMCs)增殖的抑制作用及其调节NF-κB(Nuclear Factor kappa-light-chain-enhancer of ac...目的:探究花生红衣原花青素(peanut red proanthocyanidins,PRP)对氧化低密度脂蛋白(ox-LDL)诱导的血管平滑肌细胞(Vascular Smooth Muscle Cells,VSMCs)增殖的抑制作用及其调节NF-κB(Nuclear Factor kappa-light-chain-enhancer of activated B cells)通路的潜在机制。方法:采用体外细胞培养模型,将VSMCs分为3组:对照组(Control)、氧化低密度脂蛋白诱导组(ox-LDL)、氧化低密度脂蛋白诱导+花生红衣原花青素组(ox-LDL+PRP)。采用CCK-8法评估细胞增殖情况,流式细胞仪分析细胞周期,应用ELISA检测炎症因子TNF-α和IL-6的表达,Western blot检测NF-κB通路相关蛋白的表达情况。结果:与ox-LDL模型组相比,原花青素处理的VSMCs表现出显著的增殖抑制效果,细胞活力值明显下降(P<0.05);细胞周期检测显示:花生红衣原花青素处理组细胞在G1期的比例显著增加,而S期和G2/M期的比例则减少;同时花生红衣原花青素处理组NF-κB通路相关蛋白p65的表达水平显著下调(P<0.05),TNF-α、IL-6表达下降(均P<0.05)。结论:花生红衣原花青素能够通过抑制NF-κB通路的激活,有效减轻ox-LDL诱导的VSMCs炎症反应,抑制VSMCs增殖。展开更多
Cesium heteropolysalts Cs3PMo12O40 and HCs3PVMo11O40 were synthesized by modifying the preparation conditions in order to get materials with a much higher surface area than the original Keggin-type heteropolyacids(H3P...Cesium heteropolysalts Cs3PMo12O40 and HCs3PVMo11O40 were synthesized by modifying the preparation conditions in order to get materials with a much higher surface area than the original Keggin-type heteropolyacids(H3PMo12O40 and H4PVMo11O40).These solids were used as carriers for the dispersion of H4PVMo11O40 heteropolyacid by the incipient wetness impregnation technique.The textural and structural properties of supports and catalysts were examined by scanning electron microscopy,N2 adsorption-desorption isotherms and Raman spectroscopy.The supported catalysts were studied before and after red/ox pretreatments by X-ray photoelectron spectroscopy,which showed that both the surface composition and oxidized to reduced species ratio depend on the used carrier.The catalytic performances of these novel supported catalysts in the selective oxidation of isobutane to methacrylic acid and methacrolein were studied.The best catalytic properties were obtained when H4PVMo11O40 was supported on HCs3PVMo11O40.The isobutane conversion and yield of the desired oxygenates increased along the unsupported H4PVMo11O40【H4PVMo11O40/Cs3PMo12O40【H4PVMo11O40/HCs3PVMo11O40 series.展开更多
文摘目的:探究花生红衣原花青素(peanut red proanthocyanidins,PRP)对氧化低密度脂蛋白(ox-LDL)诱导的血管平滑肌细胞(Vascular Smooth Muscle Cells,VSMCs)增殖的抑制作用及其调节NF-κB(Nuclear Factor kappa-light-chain-enhancer of activated B cells)通路的潜在机制。方法:采用体外细胞培养模型,将VSMCs分为3组:对照组(Control)、氧化低密度脂蛋白诱导组(ox-LDL)、氧化低密度脂蛋白诱导+花生红衣原花青素组(ox-LDL+PRP)。采用CCK-8法评估细胞增殖情况,流式细胞仪分析细胞周期,应用ELISA检测炎症因子TNF-α和IL-6的表达,Western blot检测NF-κB通路相关蛋白的表达情况。结果:与ox-LDL模型组相比,原花青素处理的VSMCs表现出显著的增殖抑制效果,细胞活力值明显下降(P<0.05);细胞周期检测显示:花生红衣原花青素处理组细胞在G1期的比例显著增加,而S期和G2/M期的比例则减少;同时花生红衣原花青素处理组NF-κB通路相关蛋白p65的表达水平显著下调(P<0.05),TNF-α、IL-6表达下降(均P<0.05)。结论:花生红衣原花青素能够通过抑制NF-κB通路的激活,有效减轻ox-LDL诱导的VSMCs炎症反应,抑制VSMCs增殖。
文摘Cesium heteropolysalts Cs3PMo12O40 and HCs3PVMo11O40 were synthesized by modifying the preparation conditions in order to get materials with a much higher surface area than the original Keggin-type heteropolyacids(H3PMo12O40 and H4PVMo11O40).These solids were used as carriers for the dispersion of H4PVMo11O40 heteropolyacid by the incipient wetness impregnation technique.The textural and structural properties of supports and catalysts were examined by scanning electron microscopy,N2 adsorption-desorption isotherms and Raman spectroscopy.The supported catalysts were studied before and after red/ox pretreatments by X-ray photoelectron spectroscopy,which showed that both the surface composition and oxidized to reduced species ratio depend on the used carrier.The catalytic performances of these novel supported catalysts in the selective oxidation of isobutane to methacrylic acid and methacrolein were studied.The best catalytic properties were obtained when H4PVMo11O40 was supported on HCs3PVMo11O40.The isobutane conversion and yield of the desired oxygenates increased along the unsupported H4PVMo11O40【H4PVMo11O40/Cs3PMo12O40【H4PVMo11O40/HCs3PVMo11O40 series.
