Electron–hole(e–h)recombination is a fundamental process that governs energy dissipation and device efficiency in semiconductors.In two-dimensional(2D)materials,the formation of tightly bound excitons makes exciton-...Electron–hole(e–h)recombination is a fundamental process that governs energy dissipation and device efficiency in semiconductors.In two-dimensional(2D)materials,the formation of tightly bound excitons makes exciton-mediated e–h recombination the dominant decay pathway.In this work,nonradiative e–h recombination within excitons in monolayer MoS2 is investigated using first-principles simulations that combine nonadiabatic molecular dynamics with𝐺𝑊and real-time Bethe–Salpeter equation(BSE)propagation.A two-step process is identified:rapid intervalley redistribution induced by exchange interaction,followed by slower phonon-assisted recombination facilitated by exciton binding.By selectively removing the screened Coulomb and exchange terms from the BSE Hamiltonian,their respective contributions are disentangled—exchange interaction is found to increase the number of accessible recombination pathways,while binding reduces the excitation energy and enhances nonradiative decay.A reduction in recombination lifetime by over an order of magnitude is observed due to the excitonic many-body effects.These findings provide microscopic insights for understanding and tuning exciton lifetimes in 2D transition-metal dichalcogenides.展开更多
Objective:Patients with homologous recombination deficiency(HRD)demonstrate distinct clinicopathological and prognostic features.However,standardised and clinically validated HRD detection methodologies specifically t...Objective:Patients with homologous recombination deficiency(HRD)demonstrate distinct clinicopathological and prognostic features.However,standardised and clinically validated HRD detection methodologies specifically tailored for non-small cell lung cancer(NSCLC)have yet to be established.Further research is needed to clarify the precise role and clinical implications of HRD in NSCLC.Methods:A cohort of 580 treatment-naive NSCLC patients was retrospectively enrolled.Comprehensive genomic profiling(CGP)was performed for all patients,and HRD status was evaluated using two genomic scar score(GSS)-based algorithms:a machine learning-based GSS(ML-GSS)and a continuous linear regression-based GSS(CLR-GSS).To assess the diagnostic performance(sensitivity and specificity)of the ML-GSS and CLR-GSS algorithms for HRD detection,immunohistochemical(IHC)staining was conducted for two HRD-related biomarkers:Schlafen 11(SLFN11)and RAD51.Survival analysis,including progression-free survival(PFS),along with multivariable Cox proportional hazards models,was performed to compare the prognostic value of the two HRD algorithms.Results:Among all patients,146(25.2%)and 46(7.9%)were classified as HRD-positive(HRD+)by ML-GSS and CLR-GSS,respectively.Using SLFN11 IHC expression as the reference standard,comparative analysis demonstrated that ML-GSS exhibited significantly higher sensitivity but lower specificity than CLR-GSS.This trend was consistently observed in RAD51 staining analysis.Compared to HRD-negative(HRD-)patients,MLGSS-defined HRD+cases displayed distinct clinicopathological and genomic features,including a higher prevalence of homologous recombination(HR)-related genes mutations,BRCA1/2 mutations,TP53 mutations,elevated tumor mutation burden(TMB),and increased copy number variations(CNVs).In contrast,CLR-GSSdefined HRD+patients were only enriched for BRCA1/2 mutations,TP53 mutations,and elevated TMB.Furthermore,ML-GSS-defined HRD+status was associated with significantly worse prognosis following first-line therapy compared to HRD-patients.Univariate and multivariable Cox analyses identified ML-GSS-defined HRD+and TP53 mutations as significant predictors and independent risk factors,respectively.No such associations were observed in the CLR-GSS-defined HRD+cohort.Conclusions:ML-GSS demonstrated superior performance to CLR-GSS in assessing chromosomal instability(CIN)and showed greater clinical utility.We recommend the ML-GSS algorithm as a robust and clinically validated tool for HRD/CIN evaluation in NSCLC.Furthermore,ML-GSS-defined HRD+status was identified as both a significant predictor and an independent risk factor.展开更多
Single-stranded DNA-binding proteins(SSBs)play essential roles in the replication,recombination and repair processes of organellar DNA molecules.In Arabidopsis thaliana,SSBs are encoded by a small family of two genes(...Single-stranded DNA-binding proteins(SSBs)play essential roles in the replication,recombination and repair processes of organellar DNA molecules.In Arabidopsis thaliana,SSBs are encoded by a small family of two genes(SSB1 and SSB2).However,the functional divergence of these two SSB copies in plants remains largely unknown,and detailed studies regarding their roles in the replication and recombination of organellar genomes are still incomplete.In this study,phylogenetic,gene structure and protein motif analyses all suggested that SSB1 and SSB2 probably diverged during the early evolution of seed plants.Based on accurate long-read sequencing results,ssb1 and ssb2 mutants had decreased copy numbers for both mitochondrial DNA(mtDNA)and plastid DNA(ptDNA),accompanied by a slight increase in structural rearrangements mediated by intermediate-sized repeats in mt genome and small-scale variants in both genomes.Our findings provide an important foundation for further investigating the effects of DNA dosage in the regulation of mutation frequencies in plant organellar genomes.展开更多
BACKGROUND Patients with colorectal cancer(CRC)exhibiting microsatellite instability(MSI)-high generally demonstrate a favorable response to immunotherapy.In contrast,the efficacy of immunotherapy in microsatellite-st...BACKGROUND Patients with colorectal cancer(CRC)exhibiting microsatellite instability(MSI)-high generally demonstrate a favorable response to immunotherapy.In contrast,the efficacy of immunotherapy in microsatellite-stable(MSS)CRC patients is considerably restricted.This study sought to evaluate the effectiveness of immu-notherapy in MSS patients characterized by homologous recombination defi-ciency(HRD)as opposed to those with homologous recombination proficiency(HRP).AIM To investigate and compare the clinicopathological characteristics,treatment modalities,and outcomes between the HRD and HRP groups in CRC.METHODS Next-generation sequencing was performed on 268 CRC patients to identify tumor-associated genetic alterations and assess their HRD scores and MSI status.Patients with HRD-related gene alterations or an HRD score≥30 were classified into the HRD group,while the remaining patients were assigned to the HRP group.Clinical data,including staging and treatment regimens,were collected for analysis.Cox regression and Kaplan-Meier survival curves were employed to evaluate whether the HRD group demonstrated improved survival outcomes following immunotherapy treatment.RESULTS Among the 268 patients,64 were classified into the HRD group,which had a higher proportion of early-stage CRC diagnoses compared to the HRP group.Kaplan-Meier survival curves indicated significantly better survival rates in the HRD group compared to the HRP group across all cohorts,as well as among MSS patients treated with immunotherapy(P<0.05).CONCLUSION This study demonstrates that CRC patients with HRD have a more favorable prognosis and suggests that HRD status could serve as a predictive marker for immunotherapy response in MSS patients.展开更多
Genome rearrangement is an important process that leads to genetic diversity,including mutation-related insertions,deletions,or inversions in the genome[1,2].
Metal halide perovskites have rapidly emerged as outstanding semiconductors for laser applications.Surface plasmon resonances of metals offer a platform for improving the perovskite lasing properties of metal halide p...Metal halide perovskites have rapidly emerged as outstanding semiconductors for laser applications.Surface plasmon resonances of metals offer a platform for improving the perovskite lasing properties of metal halide perovskites by accelerating radiative recombination.However,the constraint on degrees of freedom of perovskite-metal interactions in two dimensions keeps us from getting a full picture of plasmon-involved carrier dynamics and reaching the optimum perovskite lasing performance.Here we report a strategy of synthesizing quantitative coassemblies of perovskite and metal nanocrystals for studying the effect of surface plasmons on carrier dynamics in depth and exploring plasmon-enhanced perovskite lasing performance.Within the coassembly,each metal nanocrystal supports localized surface plasmon resonances capable of accelerating radiative recombination of all adjacent perovskite nanocrystals in three dimensions.The quantitative coassemblies disclose the evolution of radiative and nonradiative recombination processes in perovskite nanocrystals with the plasmon modes,identifying an optimal metal nanocrystal content for fulfilling the highest radiative efficiency in perovskite nanocrystals.By virtue of accelerated radiative recombination,the coassemblies of perovskite and metal nanocrystals allowed for the construction of microlaser arrays with enhanced performance including low thresholds and ultrafast outputs.This work fundamentally advances the perovskite-metal systems for plasmonically enhancing perovskite optoelectronic performance.展开更多
In this study, RT-PCR was performed on lung tissue samples from sick pigs in a suspected outbreak of porcine reproductive and respiratory syndrome (PRRS) at a pig farm in Mianyang City, Sichuan Province, China. Positi...In this study, RT-PCR was performed on lung tissue samples from sick pigs in a suspected outbreak of porcine reproductive and respiratory syndrome (PRRS) at a pig farm in Mianyang City, Sichuan Province, China. Positive samples were inoculated into Marc-145 cells to observe lesions. The Marc-145 cells with cytopathic lesions were identified by indirect immunofluorescence. The whole genome sequences of the isolated and purified strains were amplified by RT-PCR and analyzed for homology and genetic evolution. A strain of porcine reproductive and respiratory syndrome virus (PRRSV), named SCMY2023 (GenBank No. PQ179742), was successfully isolated. SCMY2023 has a genome length of 15,321 base pairs (without a poly A tail). Nucleotide and amino acid homology analyses suggest that this strain belongs to Lineage 8, a variant of the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) prevalent in China. Recombination and genetic evolution analyses indicate that this isolate is a PRRSV variant that recombined with HuN-ZZ (Lineage 8, 98.79% homology) on the backbone of the SCSN2020 strain (Lineage 8, 99.35% homology) in the recombination region from 4407 to 13,107 nucleotides (ORF1a to ORF3). In-depth study of the genetic recombination of this isolate can provide a reference for the prevention and control of PRRS.展开更多
目的探讨小剂量多巴胺联合重组人脑利钠肽(recombinant human brain natriuretic peptide,rhBNP)治疗心肌梗死(MI)后心力衰竭(HF)的临床效果及对患者系统性免疫炎症指数(SII)、三酰甘油葡萄糖乘积(TyG)指数的影响。方法选取64例MI后HF患...目的探讨小剂量多巴胺联合重组人脑利钠肽(recombinant human brain natriuretic peptide,rhBNP)治疗心肌梗死(MI)后心力衰竭(HF)的临床效果及对患者系统性免疫炎症指数(SII)、三酰甘油葡萄糖乘积(TyG)指数的影响。方法选取64例MI后HF患者,按随机数字表法分为对照组与观察组各32例,患者在入院后均给予常规治疗,对照组在常规治疗基础上给予rhBNP治疗,观察组在常规治疗基础上给予小剂量多巴胺联合rhBNP治疗。治疗7d后,观察2组患者血清SII、TyG指数与心功能指标[左室射血分数(LVEF)、前负荷率(EDFR)、外周血管阻力(SVR)]变化及临床效果、不良反应和主要不良心血管事件(MACE)发生情况。结果观察组患者治疗后SII、TyG水平低于对照组(P<0.05);心功能指标LVEF、SVR高于对照组,EDFR低于对照组(P<0.05)。观察组治疗总有效率(96.88%)高于对照组(84.38%)(P<0.05);治疗后6个月MACE发生率观察组(3.13%)低于对照组(18.75%)(P<0.05);用药不良反应发生率2组间比较差异无统计学意义(P>0.05)。结论采取小剂量多巴胺联合rhBNP用药方案治疗MI后HF,改善患者临床疗效、炎症状态及心功能较单一使用rhBNP效果更好,不明显增加不良事件发生率,值得基层医院参考应用。展开更多
Many eukaryotic genes are members of multi-gene families due to gene duplications, which generate new copies that allow functional divergence. However, the relationship between
The published article titled“Truncated Bid Overexpression Induced by Recombinant Adenovirus Cre/LoxP System Suppresses the Tumorigenic Potential of CD133+Ovarian Cancer Stem Cells”has been retracted from Oncology Re...The published article titled“Truncated Bid Overexpression Induced by Recombinant Adenovirus Cre/LoxP System Suppresses the Tumorigenic Potential of CD133+Ovarian Cancer Stem Cells”has been retracted from Oncology Research,Vol.25,No.4,2017,pp.595–603.展开更多
Recombinant tissue plasminogen activator is commonly used for hematoma evacuation in minimally invasive surgery following intracerebral hemorrhage.However,during minimally invasive surgery,recombinant tissue plasminog...Recombinant tissue plasminogen activator is commonly used for hematoma evacuation in minimally invasive surgery following intracerebral hemorrhage.However,during minimally invasive surgery,recombinant tissue plasminogen activator may come into contact with brain tissue.Therefore,a thorough assessment of its safety is required.In this study,we established a mouse model of intracerebral hemorrhage induced by type VII collagenase.We observed that the administration of recombinant tissue plasminogen activator without hematoma aspiration significantly improved the neurological function of mice with intracerebral hemorrhage,reduced pathological damage,and lowered the levels of apoptosis and autophagy in the tissue surrounding the hematoma.In an in vitro model of intracerebral hemorrhage using primary cortical neurons induced by hemin,the administration of recombinant tissue plasminogen activator suppressed neuronal apoptosis,autophagy,and endoplasmic reticulum stress.Transcriptome sequencing analysis revealed that recombinant tissue plasminogen activator upregulated the phosphoinositide 3-kinase/RAC-alpha serine/threonine-protein kinase/mammalian target of rapamycin pathway in neurons.Moreover,the phosphoinositide 3-kinase inhibitor LY294002 abrogated the neuroprotective effects of recombinant tissue plasminogen activator in inhibiting excessive apoptosis,autophagy,and endoplasmic reticulum stress.Furthermore,to specify the domain of recombinant tissue plasminogen activator responsible for its neuroprotective effects,various inhibitors were used to target distinct domains.It has been revealed that the epidermal growth factor receptor inhibitor AG-1478 reversed the effect of recombinant tissue plasminogen activator on the phosphoinositide 3-kinase/RAC-alpha serine/threonineprotein kinase/mammalian target of rapamycin pathway.These findings suggest that recombinant tissue plasminogen activator exerts a direct neuroprotective effect on neurons following intracerebral hemorrhage,possibly through activation of the phosphoinositide 3-kinase/RAC-alpha serine/threonine-protein kinase/mammalian target of rapamycin pathway.展开更多
DNA is the genetic material of all cells, containing coded information about cellular molecules and processes. DNA consists of two polynucleofide strands twisted around each other in a double helix. The first step in ...DNA is the genetic material of all cells, containing coded information about cellular molecules and processes. DNA consists of two polynucleofide strands twisted around each other in a double helix. The first step in cellular division is to replicate DNA so that copies can be distributed to daughter cells. Additionally, DNA is involved in transcribing proteins that direct cell growth and activities. However, DNA is tightly packed into genes and chromosomes. In order for replication or transcription to take place, DNA must firstly unpack itself so that it can interact with enzymes. DNA packing can be visualized as two very long strands that have been intertwined millions of times, tied into knots, and subjected to successive coiling. However, replication and transcription are much easier to accomplish if the DNA is neatly arranged rather than tangled up in knots. Enzymes are essential to unpacking DNA. Enzymes act to slice through individual knots and reconnect strands in a more orderly way. Hypothesizing that Termination of DNA replication proteins gave rise to those of eukaryotes during evolution, we chose the DNA polymerase （which infects microalgae） as the basis of this analysis, as it represents a primitive recombination. We show that it has significant similarity with replicative DNA polymerases of eukaryotes and certain of their large DNA. Sequence alignment confirms this similarity and establishes the presence of highly conserved domains in the polymerase amino terminus. Subsequent reconstruction of a phylogenetic tree indicates that these algal DNA are near the root of the containing all recombination. DNA polymerase delta members but that this does not contain the polymerases of other DNA. We consider arguments for the polarity of this relationship and present the hypothesis that the replication genes of DNA. DNA can be visualized as a complicated knot that must be unknotted by enzymes in order for replication or transcription to occur. It is perhaps not surprising then that connections between mathematical knot theory and biology have been discovered. By thinking of DNA as a knot, we can use knot theory to estimate how hard DNA is to unknot. This can help us estimate properties of the enzymes that unknot DNA.展开更多
基金supported by the National Key Research and Development Program of China (Grant Nos.2024YFA1409800 for J.Z.and2024YFA1408603 for Q.Z.)the National Natural Science Foundation of China (Grant Nos.12125408,12334004for J.Z.,and 12174363 for Q.Z.)+1 种基金the Innovation Program for Quantum Science and Technology (Grant No.2021ZD0303306 for J.Z.)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB0450101 for J.Z.)。
文摘Electron–hole(e–h)recombination is a fundamental process that governs energy dissipation and device efficiency in semiconductors.In two-dimensional(2D)materials,the formation of tightly bound excitons makes exciton-mediated e–h recombination the dominant decay pathway.In this work,nonradiative e–h recombination within excitons in monolayer MoS2 is investigated using first-principles simulations that combine nonadiabatic molecular dynamics with𝐺𝑊and real-time Bethe–Salpeter equation(BSE)propagation.A two-step process is identified:rapid intervalley redistribution induced by exchange interaction,followed by slower phonon-assisted recombination facilitated by exciton binding.By selectively removing the screened Coulomb and exchange terms from the BSE Hamiltonian,their respective contributions are disentangled—exchange interaction is found to increase the number of accessible recombination pathways,while binding reduces the excitation energy and enhances nonradiative decay.A reduction in recombination lifetime by over an order of magnitude is observed due to the excitonic many-body effects.These findings provide microscopic insights for understanding and tuning exciton lifetimes in 2D transition-metal dichalcogenides.
基金supported by the National High Level Hospital Clinical Research Funding(No.BJ-2019-195)the National High Level Hospital Clinical Research Funding(No.BJ-2023-090)。
文摘Objective:Patients with homologous recombination deficiency(HRD)demonstrate distinct clinicopathological and prognostic features.However,standardised and clinically validated HRD detection methodologies specifically tailored for non-small cell lung cancer(NSCLC)have yet to be established.Further research is needed to clarify the precise role and clinical implications of HRD in NSCLC.Methods:A cohort of 580 treatment-naive NSCLC patients was retrospectively enrolled.Comprehensive genomic profiling(CGP)was performed for all patients,and HRD status was evaluated using two genomic scar score(GSS)-based algorithms:a machine learning-based GSS(ML-GSS)and a continuous linear regression-based GSS(CLR-GSS).To assess the diagnostic performance(sensitivity and specificity)of the ML-GSS and CLR-GSS algorithms for HRD detection,immunohistochemical(IHC)staining was conducted for two HRD-related biomarkers:Schlafen 11(SLFN11)and RAD51.Survival analysis,including progression-free survival(PFS),along with multivariable Cox proportional hazards models,was performed to compare the prognostic value of the two HRD algorithms.Results:Among all patients,146(25.2%)and 46(7.9%)were classified as HRD-positive(HRD+)by ML-GSS and CLR-GSS,respectively.Using SLFN11 IHC expression as the reference standard,comparative analysis demonstrated that ML-GSS exhibited significantly higher sensitivity but lower specificity than CLR-GSS.This trend was consistently observed in RAD51 staining analysis.Compared to HRD-negative(HRD-)patients,MLGSS-defined HRD+cases displayed distinct clinicopathological and genomic features,including a higher prevalence of homologous recombination(HR)-related genes mutations,BRCA1/2 mutations,TP53 mutations,elevated tumor mutation burden(TMB),and increased copy number variations(CNVs).In contrast,CLR-GSSdefined HRD+patients were only enriched for BRCA1/2 mutations,TP53 mutations,and elevated TMB.Furthermore,ML-GSS-defined HRD+status was associated with significantly worse prognosis following first-line therapy compared to HRD-patients.Univariate and multivariable Cox analyses identified ML-GSS-defined HRD+and TP53 mutations as significant predictors and independent risk factors,respectively.No such associations were observed in the CLR-GSS-defined HRD+cohort.Conclusions:ML-GSS demonstrated superior performance to CLR-GSS in assessing chromosomal instability(CIN)and showed greater clinical utility.We recommend the ML-GSS algorithm as a robust and clinically validated tool for HRD/CIN evaluation in NSCLC.Furthermore,ML-GSS-defined HRD+status was identified as both a significant predictor and an independent risk factor.
基金supported by grants from the National Natural Science Foundation of China(32170238,32400191)Guangdong Basic and Applied Basic Research Foundation(2023A1515111029)+2 种基金the Science,Technology and Innovation Commission of Shenzhen Municipality(RCYX20200714114538196)the Chinese Academy of Agricultural Sciences Elite Youth Program(grant 110243160001007)the Guangdong Pearl River Talent Program(2021QN02N792)。
文摘Single-stranded DNA-binding proteins(SSBs)play essential roles in the replication,recombination and repair processes of organellar DNA molecules.In Arabidopsis thaliana,SSBs are encoded by a small family of two genes(SSB1 and SSB2).However,the functional divergence of these two SSB copies in plants remains largely unknown,and detailed studies regarding their roles in the replication and recombination of organellar genomes are still incomplete.In this study,phylogenetic,gene structure and protein motif analyses all suggested that SSB1 and SSB2 probably diverged during the early evolution of seed plants.Based on accurate long-read sequencing results,ssb1 and ssb2 mutants had decreased copy numbers for both mitochondrial DNA(mtDNA)and plastid DNA(ptDNA),accompanied by a slight increase in structural rearrangements mediated by intermediate-sized repeats in mt genome and small-scale variants in both genomes.Our findings provide an important foundation for further investigating the effects of DNA dosage in the regulation of mutation frequencies in plant organellar genomes.
基金Supported by Natural Science Foundation of Guangdong Province,No.2021A1515011146 and No.2023A1515010785Key Areas Research and Development Programs of Guangdong Province,No.2023B1111050009.
文摘BACKGROUND Patients with colorectal cancer(CRC)exhibiting microsatellite instability(MSI)-high generally demonstrate a favorable response to immunotherapy.In contrast,the efficacy of immunotherapy in microsatellite-stable(MSS)CRC patients is considerably restricted.This study sought to evaluate the effectiveness of immu-notherapy in MSS patients characterized by homologous recombination defi-ciency(HRD)as opposed to those with homologous recombination proficiency(HRP).AIM To investigate and compare the clinicopathological characteristics,treatment modalities,and outcomes between the HRD and HRP groups in CRC.METHODS Next-generation sequencing was performed on 268 CRC patients to identify tumor-associated genetic alterations and assess their HRD scores and MSI status.Patients with HRD-related gene alterations or an HRD score≥30 were classified into the HRD group,while the remaining patients were assigned to the HRP group.Clinical data,including staging and treatment regimens,were collected for analysis.Cox regression and Kaplan-Meier survival curves were employed to evaluate whether the HRD group demonstrated improved survival outcomes following immunotherapy treatment.RESULTS Among the 268 patients,64 were classified into the HRD group,which had a higher proportion of early-stage CRC diagnoses compared to the HRP group.Kaplan-Meier survival curves indicated significantly better survival rates in the HRD group compared to the HRP group across all cohorts,as well as among MSS patients treated with immunotherapy(P<0.05).CONCLUSION This study demonstrates that CRC patients with HRD have a more favorable prognosis and suggests that HRD status could serve as a predictive marker for immunotherapy response in MSS patients.
基金supported by grants(92168103,32171417,2019CXJQ01)from the National Nature Science Foundation of China,Shanghai Municipal GovernmentPeak Disciplines(Type IV)of Institutions of Higher Learning in Shanghai.
文摘Genome rearrangement is an important process that leads to genetic diversity,including mutation-related insertions,deletions,or inversions in the genome[1,2].
基金supported by the National Natural Science Foundation of China(Nos.52272186,22090023 and 22375207)Beijing Institute of Technology Research Fund Program for Young Scholars(No.XSQD-6120220081)
文摘Metal halide perovskites have rapidly emerged as outstanding semiconductors for laser applications.Surface plasmon resonances of metals offer a platform for improving the perovskite lasing properties of metal halide perovskites by accelerating radiative recombination.However,the constraint on degrees of freedom of perovskite-metal interactions in two dimensions keeps us from getting a full picture of plasmon-involved carrier dynamics and reaching the optimum perovskite lasing performance.Here we report a strategy of synthesizing quantitative coassemblies of perovskite and metal nanocrystals for studying the effect of surface plasmons on carrier dynamics in depth and exploring plasmon-enhanced perovskite lasing performance.Within the coassembly,each metal nanocrystal supports localized surface plasmon resonances capable of accelerating radiative recombination of all adjacent perovskite nanocrystals in three dimensions.The quantitative coassemblies disclose the evolution of radiative and nonradiative recombination processes in perovskite nanocrystals with the plasmon modes,identifying an optimal metal nanocrystal content for fulfilling the highest radiative efficiency in perovskite nanocrystals.By virtue of accelerated radiative recombination,the coassemblies of perovskite and metal nanocrystals allowed for the construction of microlaser arrays with enhanced performance including low thresholds and ultrafast outputs.This work fundamentally advances the perovskite-metal systems for plasmonically enhancing perovskite optoelectronic performance.
文摘In this study, RT-PCR was performed on lung tissue samples from sick pigs in a suspected outbreak of porcine reproductive and respiratory syndrome (PRRS) at a pig farm in Mianyang City, Sichuan Province, China. Positive samples were inoculated into Marc-145 cells to observe lesions. The Marc-145 cells with cytopathic lesions were identified by indirect immunofluorescence. The whole genome sequences of the isolated and purified strains were amplified by RT-PCR and analyzed for homology and genetic evolution. A strain of porcine reproductive and respiratory syndrome virus (PRRSV), named SCMY2023 (GenBank No. PQ179742), was successfully isolated. SCMY2023 has a genome length of 15,321 base pairs (without a poly A tail). Nucleotide and amino acid homology analyses suggest that this strain belongs to Lineage 8, a variant of the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) prevalent in China. Recombination and genetic evolution analyses indicate that this isolate is a PRRSV variant that recombined with HuN-ZZ (Lineage 8, 98.79% homology) on the backbone of the SCSN2020 strain (Lineage 8, 99.35% homology) in the recombination region from 4407 to 13,107 nucleotides (ORF1a to ORF3). In-depth study of the genetic recombination of this isolate can provide a reference for the prevention and control of PRRS.
文摘目的探讨小剂量多巴胺联合重组人脑利钠肽(recombinant human brain natriuretic peptide,rhBNP)治疗心肌梗死(MI)后心力衰竭(HF)的临床效果及对患者系统性免疫炎症指数(SII)、三酰甘油葡萄糖乘积(TyG)指数的影响。方法选取64例MI后HF患者,按随机数字表法分为对照组与观察组各32例,患者在入院后均给予常规治疗,对照组在常规治疗基础上给予rhBNP治疗,观察组在常规治疗基础上给予小剂量多巴胺联合rhBNP治疗。治疗7d后,观察2组患者血清SII、TyG指数与心功能指标[左室射血分数(LVEF)、前负荷率(EDFR)、外周血管阻力(SVR)]变化及临床效果、不良反应和主要不良心血管事件(MACE)发生情况。结果观察组患者治疗后SII、TyG水平低于对照组(P<0.05);心功能指标LVEF、SVR高于对照组,EDFR低于对照组(P<0.05)。观察组治疗总有效率(96.88%)高于对照组(84.38%)(P<0.05);治疗后6个月MACE发生率观察组(3.13%)低于对照组(18.75%)(P<0.05);用药不良反应发生率2组间比较差异无统计学意义(P>0.05)。结论采取小剂量多巴胺联合rhBNP用药方案治疗MI后HF,改善患者临床疗效、炎症状态及心功能较单一使用rhBNP效果更好,不明显增加不良事件发生率,值得基层医院参考应用。
文摘Many eukaryotic genes are members of multi-gene families due to gene duplications, which generate new copies that allow functional divergence. However, the relationship between
文摘The published article titled“Truncated Bid Overexpression Induced by Recombinant Adenovirus Cre/LoxP System Suppresses the Tumorigenic Potential of CD133+Ovarian Cancer Stem Cells”has been retracted from Oncology Research,Vol.25,No.4,2017,pp.595–603.
基金supported by the National Natural Science Foundation of China,Nos.92148206,82071330(both to ZT)a grant from the Major Program of Hubei Province,No.2023BAA005(to ZT)+1 种基金a grant from the Key Research and Discovery Program of Hubei Province,No.2021BCA109(to ZT)the Research Foundation of Tongji Hospital,No.2022B37(to PZ)。
文摘Recombinant tissue plasminogen activator is commonly used for hematoma evacuation in minimally invasive surgery following intracerebral hemorrhage.However,during minimally invasive surgery,recombinant tissue plasminogen activator may come into contact with brain tissue.Therefore,a thorough assessment of its safety is required.In this study,we established a mouse model of intracerebral hemorrhage induced by type VII collagenase.We observed that the administration of recombinant tissue plasminogen activator without hematoma aspiration significantly improved the neurological function of mice with intracerebral hemorrhage,reduced pathological damage,and lowered the levels of apoptosis and autophagy in the tissue surrounding the hematoma.In an in vitro model of intracerebral hemorrhage using primary cortical neurons induced by hemin,the administration of recombinant tissue plasminogen activator suppressed neuronal apoptosis,autophagy,and endoplasmic reticulum stress.Transcriptome sequencing analysis revealed that recombinant tissue plasminogen activator upregulated the phosphoinositide 3-kinase/RAC-alpha serine/threonine-protein kinase/mammalian target of rapamycin pathway in neurons.Moreover,the phosphoinositide 3-kinase inhibitor LY294002 abrogated the neuroprotective effects of recombinant tissue plasminogen activator in inhibiting excessive apoptosis,autophagy,and endoplasmic reticulum stress.Furthermore,to specify the domain of recombinant tissue plasminogen activator responsible for its neuroprotective effects,various inhibitors were used to target distinct domains.It has been revealed that the epidermal growth factor receptor inhibitor AG-1478 reversed the effect of recombinant tissue plasminogen activator on the phosphoinositide 3-kinase/RAC-alpha serine/threonineprotein kinase/mammalian target of rapamycin pathway.These findings suggest that recombinant tissue plasminogen activator exerts a direct neuroprotective effect on neurons following intracerebral hemorrhage,possibly through activation of the phosphoinositide 3-kinase/RAC-alpha serine/threonine-protein kinase/mammalian target of rapamycin pathway.
文摘DNA is the genetic material of all cells, containing coded information about cellular molecules and processes. DNA consists of two polynucleofide strands twisted around each other in a double helix. The first step in cellular division is to replicate DNA so that copies can be distributed to daughter cells. Additionally, DNA is involved in transcribing proteins that direct cell growth and activities. However, DNA is tightly packed into genes and chromosomes. In order for replication or transcription to take place, DNA must firstly unpack itself so that it can interact with enzymes. DNA packing can be visualized as two very long strands that have been intertwined millions of times, tied into knots, and subjected to successive coiling. However, replication and transcription are much easier to accomplish if the DNA is neatly arranged rather than tangled up in knots. Enzymes are essential to unpacking DNA. Enzymes act to slice through individual knots and reconnect strands in a more orderly way. Hypothesizing that Termination of DNA replication proteins gave rise to those of eukaryotes during evolution, we chose the DNA polymerase （which infects microalgae） as the basis of this analysis, as it represents a primitive recombination. We show that it has significant similarity with replicative DNA polymerases of eukaryotes and certain of their large DNA. Sequence alignment confirms this similarity and establishes the presence of highly conserved domains in the polymerase amino terminus. Subsequent reconstruction of a phylogenetic tree indicates that these algal DNA are near the root of the containing all recombination. DNA polymerase delta members but that this does not contain the polymerases of other DNA. We consider arguments for the polarity of this relationship and present the hypothesis that the replication genes of DNA. DNA can be visualized as a complicated knot that must be unknotted by enzymes in order for replication or transcription to occur. It is perhaps not surprising then that connections between mathematical knot theory and biology have been discovered. By thinking of DNA as a knot, we can use knot theory to estimate how hard DNA is to unknot. This can help us estimate properties of the enzymes that unknot DNA.
