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Fuzzy Adaptive Admittance Control of Hexapod Wheeled-Legged Robot Based on Real-Time Estimation
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作者 CHEN Mengqi LI Yan XU Yang 《Journal of Donghua University(English Edition)》 2025年第6期650-660,共11页
A fuzzy adaptive admittance control method based on real-time estimation is proposed for the motion of the hexapod wheeled-legged robot in various environments.Firstly,the mechanical structure of the robot is designed... A fuzzy adaptive admittance control method based on real-time estimation is proposed for the motion of the hexapod wheeled-legged robot in various environments.Firstly,the mechanical structure of the robot is designed,and a control system framework is proposed according to the different motion environments.To address the adaptability issue of the robot foot contact with the ground,a position-based admittance control method is proposed.Secondly,to improve the tracking performance of the robot foot contact force when the ground environment changes,a fuzzy adaptive admittance parameter adjustment method is proposed.Furthermore,to address the problem of sudden changes in the tracking difference of the foot contact force when the ground environment changes,a real-time estimation method is proposed to estimate the dynamic foot contact force.Finally,a simulation experiment is conducted in MATLAB and Simscape to verify the effectiveness of the robot motion control system,admittance control,fuzzy adaptive admittance parameters adjustment,and the realtime estimation method.Through multi-scenario experiments with the robot prototype,the control method demonstrates its effectiveness and adaptability in various environments. 展开更多
关键词 hexapod wheeled-legged robot dynamic foot contact force fuzzy adaptive real-time estimation admittance control
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Observer-Based Nonlinear Control of A Torque Motor with Perturbation Estimation 被引量:6
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作者 E Prempain 《International Journal of Automation and computing》 EI 2006年第1期84-90,共7页
This paper presents an observer-based nonlinear control method that was developed and implemented to provide accurate tracking control of a limited angle torque motor following a 50Hz reference waveform. The method is... This paper presents an observer-based nonlinear control method that was developed and implemented to provide accurate tracking control of a limited angle torque motor following a 50Hz reference waveform. The method is based on a robust nonlinear observer, which is used to estimate system states and perturbations and then employ input-output feedbazk linearization to compensate for the system nonlinearities and uncertainties. The estimation of system states and perturbations allows input-output linearization of the nonlinear system without an accurate mathematical model of nominal plant. The simulation results show that the observer-based nonlinear control method is superior in comparison with the conventional model-based state feedback linearizing controller. 展开更多
关键词 torque motor nonlinear observer fictitious state perturbation estimation.
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A real-time prediction method for tunnel boring machine cutter-head torque using bidirectional long short-term memory networks optimized by multi-algorithm 被引量:7
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作者 Xing Huang Quantai Zhang +4 位作者 Quansheng Liu Xuewei Liu Bin Liu Junjie Wang Xin Yin 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2022年第3期798-812,共15页
Based on data from the Jilin Water Diversion Tunnels from the Songhua River(China),an improved and real-time prediction method optimized by multi-algorithm for tunnel boring machine(TBM)cutter-head torque is presented... Based on data from the Jilin Water Diversion Tunnels from the Songhua River(China),an improved and real-time prediction method optimized by multi-algorithm for tunnel boring machine(TBM)cutter-head torque is presented.Firstly,a function excluding invalid and abnormal data is established to distinguish TBM operating state,and a feature selection method based on the SelectKBest algorithm is proposed.Accordingly,ten features that are most closely related to the cutter-head torque are selected as input variables,which,in descending order of influence,include the sum of motor torque,cutter-head power,sum of motor power,sum of motor current,advance rate,cutter-head pressure,total thrust force,penetration rate,cutter-head rotational velocity,and field penetration index.Secondly,a real-time cutterhead torque prediction model’s structure is developed,based on the bidirectional long short-term memory(BLSTM)network integrating the dropout algorithm to prevent overfitting.Then,an algorithm to optimize hyperparameters of model based on Bayesian and cross-validation is proposed.Early stopping and checkpoint algorithms are integrated to optimize the training process.Finally,a BLSTMbased real-time cutter-head torque prediction model is developed,which fully utilizes the previous time-series tunneling information.The mean absolute percentage error(MAPE)of the model in the verification section is 7.3%,implying that the presented model is suitable for real-time cutter-head torque prediction.Furthermore,an incremental learning method based on the above base model is introduced to improve the adaptability of the model during the TBM tunneling.Comparison of the prediction performance between the base and incremental learning models in the same tunneling section shows that:(1)the MAPE of the predicted results of the BLSTM-based real-time cutter-head torque prediction model remains below 10%,and both the coefficient of determination(R^(2))and correlation coefficient(r)between measured and predicted values exceed 0.95;and(2)the incremental learning method is suitable for realtime cutter-head torque prediction and can effectively improve the prediction accuracy and generalization capacity of the model during the excavation process. 展开更多
关键词 Tunnel boring machine(TBM) real-time cutter-head torque prediction Bidirectional long short-term memory (BLSTM) Bayesian optimization Multi-algorithm fusion optimization Incremental learning
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Restoring force correction based on online discrete tangent stiffness estimation method for real-time hybrid simulation 被引量:2
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作者 Huang Liang Guo Tong +1 位作者 Chen Cheng Chen Menghui 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2018年第4期805-820,共16页
In real-time hybrid simulation(RTHS), it is difficult if not impossible to completely erase the error in restoring force due to actuator response delay using existing displacement-based compensation methods. This pa... In real-time hybrid simulation(RTHS), it is difficult if not impossible to completely erase the error in restoring force due to actuator response delay using existing displacement-based compensation methods. This paper proposes a new force correction method based on online discrete tangent stiffness estimation(online DTSE) to provide accurate online estimation of the instantaneous stiffness of the physical substructure. Following the discrete curve parameter recognition theory, the online DTSE method estimates the instantaneous stiffness mainly through adaptively building a fuzzy segment with the latest measurements, constructing several strict bounding lines of the segment and calculating the slope of the strict bounding lines, which significantly improves the calculation efficiency and accuracy for the instantaneous stiffness estimation. The results of both computational simulation and real-time hybrid simulation show that:(1) the online DTSE method has high calculation efficiency, of which the relatively short computation time will not interrupt RTHS; and(2) the online DTSE method provides better estimation for the instantaneous stiffness, compared with other existing estimation methods. Due to the quick and accurate estimation of instantaneous stiffness, the online DTSE method therefore provides a promising technique to correct restoring forces in RTHS. 展开更多
关键词 online discrete tangent stiffness estimation restoring force correction fuzzy segment parameter updating real-time hybrid simulation
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Torque estimation for robotic joint with harmonic drive transmission based on system dynamic characteristics 被引量:1
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作者 ZHU Minghong XIAO Shu YU Fei 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2022年第6期1320-1331,共12页
In the applications of joint control and robot movement,the joint torque estimation has been treated as an effective technique and widely used.Researches are made to analyze the kinematic and compliance model of the r... In the applications of joint control and robot movement,the joint torque estimation has been treated as an effective technique and widely used.Researches are made to analyze the kinematic and compliance model of the robot joint with harmonic drive to acquire high precision torque output.Through analyzing the structures of the harmonic drive and experiment apparatus,a scheme of the proposed joint torque estimation method based on both the dynamic characteristics and unscented Kalman filter(UKF)is designed and built.Based on research and scheme,torque estimation methods in view of only harmonic drive compliance model and compliance model with the Kalman filter are simulated as guidance and reference to promote the research on the torque estimation technique.Finally,a promoted torque estimation method depending on both harmonic drive compliance model and UKF is designed,and simulation results compared with the measurements of a commercial torque sensor,have verified the effectiveness of the proposed method. 展开更多
关键词 harmonic drive torque estimation compliance model unscented Kalman filter(UKF)
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Model Predictive Direct Torque Control of Permanent Magnet Synchronous Motor (PMSM) with Online Parameter Estimation Based on Extended Kalman Filter 被引量:1
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作者 Gang Yang Xiao Jiang Shuaishuai Lv 《International Journal of Communications, Network and System Sciences》 2022年第7期79-93,共15页
Aiming at the torque and flux ripples in the direct torque control and the time-varying parameters for permanent magnet synchronous motor (PMSM), a model predictive direct torque control with online parameter estimati... Aiming at the torque and flux ripples in the direct torque control and the time-varying parameters for permanent magnet synchronous motor (PMSM), a model predictive direct torque control with online parameter estimation based on the extended Kalman filter for PMSM is designed. By predicting the errors of torque and flux based on the model and the current states of the system, the optimal voltage vector is selected to minimize the error of torque and flux. The stator resistance and inductance are estimated online via EKF to reduce the effect of model error and the current estimation can reduce the error caused by measurement noise. The stability of the EKF is proved in theory. The simulation experiment results show the method can estimate the motor parameters, reduce the torque, and flux ripples and improve the performance of direct torque control for permanent magnet synchronous motor (PMSM). 展开更多
关键词 Model Predictive Direct torque Control Extended Kalman Filter Parameter estimation Permanent Magnet Synchronous Motor Filter’s Stability
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Sensor-less Terrain Estimation and Torque Control of a Wheeled Mobile Robot 被引量:1
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作者 J.D.C.C RATHNAPALA M.U.B JAYATHILAKE +2 位作者 R.M.U.K.H.M.K RATHNAYAKE B.G.L.T SAMARANAYAKE Nalin HARISCHANDRA 《Instrumentation》 2019年第2期41-48,共8页
As unmanned electric wheeled mobile robots have been increasingly applied to high-speed operations in unknown environments,the wheel slip becomes a problem when the robot is either accelerating,decelerating,or turning... As unmanned electric wheeled mobile robots have been increasingly applied to high-speed operations in unknown environments,the wheel slip becomes a problem when the robot is either accelerating,decelerating,or turning at high speed.Ignoring the effect of wheel slip may cause the mobile robot to deviate from the desired path.In this paper a recently proposed method is implemented to estimate the surface conditions encountered by an unmanned wheeled mobile robot,without using extra sensors.The method is simple,economical and needs less processing power than for other methods.A reaction torque observer is used to obtain the rolling resistance torque and it is applied to a wheeled mobile robot to obtain the surface condition in real-time for each wheel.The slip information is observed by comparing the reaction torque of each wheel.The obtained slip information is then used to control the torque of both wheels using a torque controller.Wheel slip is minimized by controlling the torque of each wheel.Minimizing the slip improves the ability of the unmanned electric wheeled mobile robot to navigate in the desired path in an unknown environment,regardless of the nature of the surface. 展开更多
关键词 WHEELED Mobile Robot Reaction torque OBSERVER DISTURBANCE OBSERVER TERRAIN estimation
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A Real-time Updated Model Predictive Control Strategy for Batch Processes Based on State Estimation
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作者 杨国军 李秀喜 钱宇 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2014年第3期318-329,共12页
Nonlinear model predictive control(NMPC) is an appealing control technique for improving the performance of batch processes, but its implementation in industry is not always possible due to its heavy on-line computati... Nonlinear model predictive control(NMPC) is an appealing control technique for improving the performance of batch processes, but its implementation in industry is not always possible due to its heavy on-line computation. To facilitate the implementation of NMPC in batch processes, we propose a real-time updated model predictive control method based on state estimation. The method includes two strategies: a multiple model building strategy and a real-time model updated strategy. The multiple model building strategy is to produce a series of sim-plified models to reduce the on-line computational complexity of NMPC. The real-time model updated strategy is to update the simplified models to keep the accuracy of the models describing dynamic process behavior. The me-thod is validated with a typical batch reactor. Simulation studies show that the new method is efficient and robust with respect to model mismatch and changes in process parameters. 展开更多
关键词 batch process exothermic batch reactor nonlinear model predictive control state estimation real-time model update
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Complete genome sequence of a Rodent Torque teno virus in Hainan Island, China and establishment of a real-time PCR for detecting Rodent Torque teno virus 3
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作者 Yue Wu Shan-Shan Wang +12 位作者 Wen-Qi Wang Huan-Huan Zhou Jin-Long Chen Yu-Fang Yi Tian-Ming Ma Xiu-Ji Cui Yi Huang Gao-Yu Wang Ruo-Yan Peng Xiao-Yuan Hu Chang-Hua He Gang Lu Fei-Fei Yin 《Journal of Hainan Medical University》 2019年第4期1-6,共6页
Objective:To perform whole-genome sequencing and phylogenetic analysis of the local endemic strain of Rodent Torque teno virus (RoTTV), RoTTV3-HMU1, found in Rattus norvegicus, Haikou City, Hainan Province, and establ... Objective:To perform whole-genome sequencing and phylogenetic analysis of the local endemic strain of Rodent Torque teno virus (RoTTV), RoTTV3-HMU1, found in Rattus norvegicus, Haikou City, Hainan Province, and establish a SYBR Green I based real-time PCR detection assay for RoTTV3.Methods: Based on the high-throughput genome sequencing analysis, specific primers were designed and the whole genome sequence was amplified by PCR and Sanger sequencing. Specific detection primers were designed based on the conserved sequences of RoTTV3. The recombinant plasmid contained the whole genome of RoTTV3-HMU1 was constructed as a standard control. The experimental conditions were optimized and the real-time PCR detection assay of RoTTV3 was established.Results: The genomic sequence of RoTTV carried by Rattus norvegicus in Haikou City was successfully sequenced. Phylogenetic analysis indicated that the virus belongs to the RoTTV3 genotype. In this experiment, the real-time PCR detection method of RoTTV3 was established. The standard curve generated had a wide dynamic range from 1×(102-108) copies/μL, with a linear correlation (R2=1.000). The melting curve analysis using SYBR Green showed only one specific melting peak and no primer-dimmers represented. The detection limit was 100 copies/reaction.Discussion: This study was the first report of the RoTTV in Hainan Islands, and its phylogenetic analysis was of great significance to the origin and evolution of RoTTV. The RoTTV3 real-time PCR detection method established in this experiment has a high sensitivity and good specificity, which lays a technical foundation for the epidemiological investigation of RoTTV3. 展开更多
关键词 RODENT torque teno virus WHOLE-GENOME SEQUENCING real-time PCR detection ASSAY
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Electromagnetic Torque Estimation of a Three-Phase Induction Motor for Setting the Feedrate of a Milling Machine
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作者 Elida Fernanda Xavier Julio Simplicio Amaud da Silva +1 位作者 Cicero da Rocha Souto Isaac Soares de Freitas 《Journal of Mechanics Engineering and Automation》 2014年第6期511-520,共10页
In this paper, it presents a project of a fuzzy controller and a neural estimator to control a coordinate table powered by three-phase induction motor, aiming to implement an intelligent milling system. The position/s... In this paper, it presents a project of a fuzzy controller and a neural estimator to control a coordinate table powered by three-phase induction motor, aiming to implement an intelligent milling system. The position/speed control is performed using vector techniques of three-phase induction machines. The estimation of the motor electromagnetic torque is used for setting the feedrate of the table. The speed control is developed using TS (Takagi-Sugeno) fuzzy logic model and electromagnetic torque estimation using neural network type LMS (least mean square) algorithm. The induction motor is powered by a frequency inverter driven by a DSP (digital signal processor). Control strategies are implemented in DSP. Simulation results are presented for evaluating the performance of the system. 展开更多
关键词 MACHINING speed control torque estimation fuzzy logic neural network
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Real-Time Maqam Estimation Model in Max/MSP Configured for the Nāy
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作者 Fadi M. Al-Ghawanmeh Mohammad T. Al-Ghawanmeh Mohammad W. Abed 《International Journal of Communications, Network and System Sciences》 2016年第2期39-52,共16页
Automatic maqam estimation is considered significant toward improving multimedia live music performances and automatic accompaniment. This contribution proposed a real-time maqam estimation model developed in the visu... Automatic maqam estimation is considered significant toward improving multimedia live music performances and automatic accompaniment. This contribution proposed a real-time maqam estimation model developed in the visual programming language MAX/MSP and configured for the nāydukah. The model’s design stood on basic formulas of Arab music maqamat as explained in theory and applied in practice. The model consisted of different layers of competition;the first was for the identification of the instant tonic of the melodic figure, and the second was for the recognition of its identifying E (E, E half-flat and E flat). Those two competitions were used to estimate the maqam in real-time. Then, accumulated estimation results were used to estimate the maqam in longer durations;five-second and full duration. The model was evaluated using professionally performed nāy improvisations. Results reflected a success in estimating all the studied maqamat when the full improvisation was considered. In addition, results were very good for real-time and five-second estimation where average estimation confidence was 75.98% and 80.04%, respectively. 展开更多
关键词 real-time Music Systems Arab Music Maqam estimation Nāy Music Signal Processing
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Position Sensorless Torque Estimation of Synchronous Reluctance Motors Based on Extended-Flux Model without Core-Loss Measurement
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作者 Masaru Hasegawa Hiroki Kitahara Atsushi Matsumoto 《Journal of Energy and Power Engineering》 2012年第9期1480-1487,共8页
This paper proposes an extended-flux model with core-loss resistance of SynRMs (synchronous reluctance motors) and precise torque estimation without core-loss measurement and position encoder. The proposed torque es... This paper proposes an extended-flux model with core-loss resistance of SynRMs (synchronous reluctance motors) and precise torque estimation without core-loss measurement and position encoder. The proposed torque estimation is useful for precise MTPA (maximum torque per ampere) control of position sensorless controlled SynRMs, which is achieved with the assistance of active and reactive powers. 展开更多
关键词 Synchronous reluctance motors maximum torque per ampere control torque estimation magnetic saturation positionsensorless.
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Real-time monitoring of disc cutter wear in tunnel boring machines:A sound and vibration sensor-based approach with machine learning technique 被引量:1
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作者 Mohammad Amir Akhlaghi Raheb Bagherpour Seyed Hadi Hoseinie 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第3期1700-1722,共23页
Large portions of the tunnel boring machine(TBM)construction cost are attributed to disc cutter consumption,and assessing the disc cutter's wear level can help determine the optimal time to replace the disc cutter... Large portions of the tunnel boring machine(TBM)construction cost are attributed to disc cutter consumption,and assessing the disc cutter's wear level can help determine the optimal time to replace the disc cutter.Therefore,the need to monitor disc cutter wear in real-time has emerged as a technical challenge for TBMs.In this study,real-time disc cutter wear monitoring is developed based on sound and vibration sensors.For this purpose,the microphone and accelerometer were used to record the sound and vibration signals of cutting three different types of rocks with varying abrasions on a laboratory scale.The relationship between disc cutter wear and the sound and vibration signal was determined by comparing the measurements of disc cutter wear with the signal plots for each sample.The features extracted from the signals showed that the sound and vibration signals are impacted by the progression of disc wear during the rock-cutting process.The signal features obtained from the rock-cutting operation were utilized to verify the machine learning techniques.The results showed that the multilayer perceptron(MLP),random subspace-based decision tree(RS-DT),DT,and random forest(RF)methods could predict the wear level of the disc cutter with an accuracy of 0.89,0.951,0.951,and 0.927,respectively.Based on the accuracy of the models and the confusion matrix,it was found that the RS-DT model has the best estimate for predicting the level of disc wear.This research has developed a method that can potentially determine when to replace a tool and assess disc wear in real-time. 展开更多
关键词 TBM disc cutter WEAR SOUND VIBRATION Machine learning real-time wear estimation
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A novel control method for turboshaft engine with variable rotor speed based on the Ngdot estimator through LQG/LTR and rotor predicted torque feedforward 被引量:5
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作者 Yong WANG Qian’gang ZHENG +1 位作者 Zhigui XU Haibo ZHANG 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2020年第7期1867-1876,共10页
In order to compensate for the disturbance of wide variation in rotor demanded torque on power turbine speed and realize the fast response control of turboshaft engine during variable rotor speed,a cascade PID control... In order to compensate for the disturbance of wide variation in rotor demanded torque on power turbine speed and realize the fast response control of turboshaft engine during variable rotor speed,a cascade PID control method based on the acceleration estimator of gas turbine speed(Ngdot)and rotor predicted torque feedforward is proposed.Firstly,a two-speed Dual Clutch Transmission(DCT)model is applied in the integrated rotor/turboshaft engine system to achieve variable rotor speed.Then,an online estimation method of Ngdot based on the Linear Quadratic Gaussian with Loop Transfer Recovery(LQG/LTR)is proposed for power turbine speed cascade control.Finally,according to the cascade PID controller based on Ngdot estimator,a rotor demanded torque predicted method based on the Min-batch Gradient Descent-Neural Network(MGD-NN)is put forward to compromise the influence of rotor torque interference.The simulation results show that compared with cascade PID controller based on Ngdot estimator and the one combined with collective pitch feedforward control,the novel control method proposed can reduce the overshoot of power turbine speed by more than 20%,which possesses faster response,superior dynamic effect and satisfactory robustness performance.The control method proposed can realize the fast response control of turboshaft engine with variable rotor speed better. 展开更多
关键词 LQG/LTR Ngdot estimator Rotor predicted torque feedforward Turboshaft engine Variable rotor speed
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RRVPE:A Robust and Real-Time Visual-Inertial-GNSS Pose Estimator for Aerial Robot Navigation 被引量:4
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作者 ZHANG Chi YANG Zhong +5 位作者 XU Hao LIAO Luwei ZHU Tang LI Guotao YANG Xin ZHANG Qiuyan 《Wuhan University Journal of Natural Sciences》 CAS CSCD 2023年第1期20-28,共9页
Self-localization and orientation estimation are the essential capabilities for mobile robot navigation.In this article,a robust and real-time visual-inertial-GNSS(Global Navigation Satellite System)tightly coupled po... Self-localization and orientation estimation are the essential capabilities for mobile robot navigation.In this article,a robust and real-time visual-inertial-GNSS(Global Navigation Satellite System)tightly coupled pose estimation(RRVPE)method for aerial robot navigation is presented.The aerial robot carries a front-facing stereo camera for self-localization and an RGB-D camera to generate 3D voxel map.Ulteriorly,a GNSS receiver is used to continuously provide pseudorange,Doppler frequency shift and universal time coordinated(UTC)pulse signals to the pose estimator.The proposed system leverages the Kanade Lucas algorithm to track Shi-Tomasi features in each video frame,and the local factor graph solution process is bounded in a circumscribed container,which can immensely abandon the computational complexity in nonlinear optimization procedure.The proposed robot pose estimator can achieve camera-rate(30 Hz)performance on the aerial robot companion computer.We thoroughly experimented the RRVPE system in both simulated and practical circumstances,and the results demonstrate dramatic advantages over the state-of-the-art robot pose estimators. 展开更多
关键词 computer vision visual-inertial-GNSS(Global Navigation Satellite System)pose estimation real-time autonomous navigation sensor fusion robotics
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An Improved Proportional Integral Estimator of the Stator Resistance for a Direct Torque Controlled Induction Motor
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作者 Keltoum Benlarbi Lakhdar Mokrani Leila Mokhnache 《Journal of Energy and Power Engineering》 2013年第11期2144-2154,共11页
In this paper, an improved PI (proportional integral) stator resistance estimation for a DTC (direct torque controlled) induction motor is proposed. This estimation method is based on an on-line stator resistance ... In this paper, an improved PI (proportional integral) stator resistance estimation for a DTC (direct torque controlled) induction motor is proposed. This estimation method is based on an on-line stator resistance correction regarding the variations of the stator current estimation error. In fact, the input variable of the P1 estimator is the stator current estimation error. The main idea is to tune accurately the stator resistance value relatively to the evolution of the stator current estimation error gradient to avoid the drive instability and ensure the tracking of the actual value of the stator resistance. But there is an unavoidable steady state error between the filtered stator current modulus and its estimated value from the dq model of the machine which is due to pseudo random commutations of the inverter switches. This may deteriorate the performance of the proposed fuzzy stator resistance estimator. An offset has been introduced in order to overcome this problem, for different speed command values and load torques. Simulation results show that the proposed estimator was able to successfully track the actual value of the stator resistance lbr different operating conditions. 展开更多
关键词 Direct torque control PI estimator stator resistance tuning PWM inverter offset compensation.
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鼠源TTV病毒海口株的基因组分析及real-time PCR检测方法的建立 被引量:1
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作者 吴悦 王珊珊 +12 位作者 王文琪 周换换 陈锦龙 易玉芳 马天明 崔秀吉 黄艺 王高玉 彭箬岩 胡小媛 何昌华 吕刚 尹飞飞 《海南医学院学报》 CAS 2019年第4期241-246,共6页
目的:对海南省海口市褐家鼠体内发现的鼠源torque teno virus(RoTTV)地方流行株RoTTV3-HMU1进行全基因组扩增测序及遗传进化分析,并建立一种基于SYBR GreenⅠ荧光染料检测RoTTV3病毒的real-time PCR检测方法。方法:基于高通量宏病毒组... 目的:对海南省海口市褐家鼠体内发现的鼠源torque teno virus(RoTTV)地方流行株RoTTV3-HMU1进行全基因组扩增测序及遗传进化分析,并建立一种基于SYBR GreenⅠ荧光染料检测RoTTV3病毒的real-time PCR检测方法。方法:基于高通量宏病毒组测序分析结果设计特异性扩增引物,采用PCR方法进行全基因组序列拼接。并根据RoTTV3保守序列通过生物信息学分析设计特异性引物,以构建的含ddrp基因的重组质粒为标准品,优化实验条件,建立RoTTV的real-time PCR检测方法。结果:成功拼接获得一株海南省海口市褐家鼠携带的RoTTV的基因组序列,遗传进化分析表明该病毒属于RoTTV3基因型。本实验建立RoTTV3 real-time PCR检测方法,标准曲线在1×10~2~1×10~8拷贝/μL浓度时呈现良好线性关系,其扩增系数为1.000,溶解曲线只出现1特异性峰,检测灵敏度达到10~2个拷贝。结论:本实验首次获得海口地区RoTTV基因组序列,并对其进行遗传进化分析,对RoTTV的起源及进化研究具有重要的意义。本实验建立的RoTTV3 real-time PCR检测方法灵敏度高、特异性好,为RoTTV3的流行病学调查奠定技术基础。 展开更多
关键词 鼠源torque teno VIRUS 基因组扩增测序 real-time PCR检测
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A NEW HYBRID WAVELET-KALMAN FILTER METHOD FOR THE ESTIMATION OF DYNAMIC SYSTEM 被引量:2
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作者 Wen Chenglin Xie Jin +1 位作者 Zhou Funa Wen Chuanbo 《Journal of Electronics(China)》 2006年第1期139-143,共5页
A new hybrid wavelet-Kalman filter method for the estimation of dynamic system is developed, Using this method, the real-time multiscale estimation of the dynamic system is implemented, and the observation equation es... A new hybrid wavelet-Kalman filter method for the estimation of dynamic system is developed, Using this method, the real-time multiscale estimation of the dynamic system is implemented, and the observation equation established is for the object signal itself rather than its wavelet decomposition. The simulation results show that this method can be used to estimate the object's state of the stacked system, which is the foundation of multiscale data fusion; besides the performance of the new algorithm developed in the letter is almost optimal. 展开更多
关键词 real-time Multiscale estimation Dynamic system Wavelet transform Kalman filter
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FPGA Implementation of Extended Kalman Filter for Parameters Estimation of Railway Wheelset 被引量:1
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作者 Khakoo Mal Tayab Din Memon +1 位作者 Imtiaz Hussain Kalwar Bhawani Shankar Chowdhry 《Computers, Materials & Continua》 SCIE EI 2023年第2期3351-3370,共20页
It is necessary to know the status of adhesion conditions between wheel and rail for efficient accelerating and decelerating of railroad vehicle.The proper estimation of adhesion conditions and their real-time impleme... It is necessary to know the status of adhesion conditions between wheel and rail for efficient accelerating and decelerating of railroad vehicle.The proper estimation of adhesion conditions and their real-time implementation is considered a challenge for scholars.In this paper,the development of simulation model of extended Kalman filter(EKF)in MATLAB/Simulink is presented to estimate various railway wheelset parameters in different contact conditions of track.Due to concurrent in nature,the Xilinx®System-on-Chip Zynq Field Programmable Gate Array(FPGA)device is chosen to check the onboard estimation ofwheel-rail interaction parameters by using the National Instruments(NI)myRIO®development board.The NImyRIO®development board is flexible to deal with nonlinearities,uncertain changes,and fastchanging dynamics in real-time occurring in wheel-rail contact conditions during vehicle operation.The simulated dataset of the railway nonlinear wheelsetmodel is tested on FPGA-based EKF with different track conditions and with accelerating and decelerating operations of the vehicle.The proposed model-based estimation of railway wheelset parameters is synthesized on FPGA and its simulation is carried out for functional verification on FPGA.The obtained simulation results are aligned with the simulation results obtained through MATLAB.To the best of our knowledge,this is the first time study that presents the implementation of a model-based estimation of railway wheelset parameters on FPGA and its functional verification.The functional behavior of the FPGA-based estimator shows that these results are the addition of current knowledge in the field of the railway. 展开更多
关键词 Adhesion force extended kalman filter FPGA implementation railway wheelset real-time estimation wheel-rail interaction
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一种基于real-time PCR技术的TTV检测方法的建立及应用
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作者 贾毅博 王高玉 +4 位作者 邓宛心 林彩云 杨华 陈运春 尹飞飞 《海南医学院学报》 CAS 北大核心 2024年第7期489-497,共9页
目的:本研究旨在开发一种具有更高灵敏度和特异性的TTV检测技术,为揭示TTV在多种疾病过程中的作用提供重要的技术支持。方法:为了更精确、灵敏的检测TTV,本研究分析了目前公布的所有亚型的TTV基因序列,在此基础上建立了一种基于UTR区域... 目的:本研究旨在开发一种具有更高灵敏度和特异性的TTV检测技术,为揭示TTV在多种疾病过程中的作用提供重要的技术支持。方法:为了更精确、灵敏的检测TTV,本研究分析了目前公布的所有亚型的TTV基因序列,在此基础上建立了一种基于UTR区域的real-time PCR检测方法,并与文献报道应用较为广泛的PCR检测方法进行了对比。结果:本研究建立的方法在1×10^(7)~1×10^(1) copies/μL标准品浓度范围内具有良好的线性关系,相关系数为1.000,斜率为-3.446,检测下限为1×10^(1) copies/μL。重复性试验结果显示,组内变异系数为7.22%,表明本方法重复性、稳定性较强。针对30份临床样本,使用本研究建立的real-time PCR检测方法及目前被多个研究所使用的4套引物进行对比。结果表明,本研究所建立的方法灵敏度显著高于文献中报道的4种方法(P<0.01);Sanger测序结果表明,本方法检测出的30份阳性样本均为TTV,检测特异性为100%。结论:本研究采用基于TaqMan探针的real-time PCR检测方法,检测灵敏性高、覆盖基因型范围广,尤其对于TTV病毒载量较低的情况下能够进行定量检测,对于TTV病毒的致病性及作为免疫标志物的应用提供重要的技术支持。 展开更多
关键词 torque teno virus 基因组扩增测序 real-time PCR检测
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