Objective Antimicrobial resistance(AMR)has become a global concern and is especially severe in China.To effectively and reliably provide AMR data,we developed a new high-throughput real-time PCR assay based on microfl...Objective Antimicrobial resistance(AMR)has become a global concern and is especially severe in China.To effectively and reliably provide AMR data,we developed a new high-throughput real-time PCR assay based on microfluidic dynamic technology,and screened multiple AMR genes in broiler fecal samples.Methods A high-throughput real-time PCR system with an new designed integrated fluidic circuit assay were performed AMR gene detection.A total of 273 broiler fecal samples collected from two geographically separated farms were screened AMR genes.Results The new assay with limits of detection ranging from 40.9 to 8,000 copies/reaction.The sensitivity rate,specificity rate,positive predictive value,negative predictive value and correct indices were 99.30%,98.08%,95.31%,99.79%,and 0.9755,respectively.Utilizing this assay,we demonstrate that AMR genes are widely spread,with positive detection rates ranging from 0 to 97.07%in 273 broiler fecal samples.bla CTX-M,bla TEM,mcr-1,fex A,cfr,optr A,and int I1 showed over 80%prevalence.The dissemination of AMR genes was distinct between the two farms.Conclusions We successfully established a new high-throughput real-time PCR assay applicable to AMR gene surveillance from fecal samples.The widespread existence of AMR genes detected in broiler farms highlights the current and severe problem of AMR.展开更多
A new method jamming against Waveform Agile SAR/GMTI (Ground Moving Target Indication) is proposed, which is called the intermittent sampling motion scatter wave jamming. This jamming can form multi-false vivid target...A new method jamming against Waveform Agile SAR/GMTI (Ground Moving Target Indication) is proposed, which is called the intermittent sampling motion scatter wave jamming. This jamming can form multi-false vivid targets scene taking the real moving targets’ all scattering information. The intermittent sampling repeater can also repeat in the waveform agile SAR current pulses. As a clever jamming mode, this jamming may realize electronic counter measure (ECM) against wave- form agile SAR. Detailed discussions about the jamming performance are given. The processing output of the jamming is derived theoretically. The superiority of this jamming is analyzed in theory. The theoretical feasibility and validity are proved by simulation experiments.展开更多
A digital three-detector positron lifetime spectrometer was developed.It consists of a DRS4 waveform digitizing board and three La Br3scintillation detectors coupled to XP2020Q photomultiplier tubes.DRS4 waveform digi...A digital three-detector positron lifetime spectrometer was developed.It consists of a DRS4 waveform digitizing board and three La Br3scintillation detectors coupled to XP2020Q photomultiplier tubes.DRS4 waveform digitizing allows data sampling at up to 5 GSPS with high amplitude resolution,with good time scale linearity and stability.In the triple-coincidence,the new system could reach a 195 ps time resolution,which is better than the conventional analog apparatus with the same detectors.This spectrometer can be applied to the other scintillation timing measurements with picoseconds accuracy.展开更多
Flying plots detection has been the focus of relay protection in power system for a long time. With the promotion of Smart substation in our country, the number of SV devices is greatly increased. Abnormal data (flyin...Flying plots detection has been the focus of relay protection in power system for a long time. With the promotion of Smart substation in our country, the number of SV devices is greatly increased. Abnormal data (flying plot) caused by sampling device itself has brought tremendous pressure to the power system. The traditional flying plot detection algorithm has plenty of defects, such as low pertinence, low sensitivity and long sampling period. This paper proposes a new algorithm to identify flying plot by analyzing the wave form characteristics of sampling data. The traditional waveform recognition methods are combined in this algorithm. It has the concept of standard wave window and can distinguish flying plot in a short time. In addition, sine recovery algorithm is used to recover the flying plot. This paper uses PSCAD software to verify the validity of this algorithm. Simulation results show that the proposed method has high reliability.展开更多
Full waveform inversion(FWI) has been increasingly more and more important in seismology to better understand the interior structure of the Earth. FWI, by taking advantage of both the traveltime and amplitude in the d...Full waveform inversion(FWI) has been increasingly more and more important in seismology to better understand the interior structure of the Earth. FWI, by taking advantage of both the traveltime and amplitude in the data, provides high-resolution model parameters of the earth which can produce images with high resolution. However, this inversion method conventionally suffers from non-uniqueness due to many local minima of the objective function and large computing costs. In this study, we propose a new FWI method in a semi-random framework by integrating the ensemble Kalman filter and uniform sampling without replacement. Numerical results demonstrate that the new method can achieve highresolution results and a wider convergence domain. Accordingly, the new method overcomes the disadvantage of conventional FWIs that depend strongly on the initial model.展开更多
针对间歇采样转发干扰产生的假目标和目标高速运动产生的多普勒频移导致雷达脉压性能急剧下降的问题,提出一种高多普勒容限的线性调频离散相位编码(linear frequency modulation-discrete phase coding,LFM-DPC)复合调制相干波形集设计...针对间歇采样转发干扰产生的假目标和目标高速运动产生的多普勒频移导致雷达脉压性能急剧下降的问题,提出一种高多普勒容限的线性调频离散相位编码(linear frequency modulation-discrete phase coding,LFM-DPC)复合调制相干波形集设计方法。在一定多普勒频移范围内,以最小化未转发信号自模糊函数旁瓣能量以及未转发信号与转发信号互模糊函数能量建立优化模型,并设计一种基于KKT(Karush-Kuhn-Tucker)最优性条件的迭代算法对模型求解。仿真实验表明,相比于遗传算法和单一调制的LFM和DPC信号,基于KKT最优性条件的交替迭代优化算法优化的LFM-DPC波形集有更好的抗间歇采样转发干扰性能。展开更多
With the recent development of diversity electro-optic sampling(DEOS),significant progress has been made in the range of applicability of single-shot EOS measurements,allowing broadband THz waveforms to be captured in...With the recent development of diversity electro-optic sampling(DEOS),significant progress has been made in the range of applicability of single-shot EOS measurements,allowing broadband THz waveforms to be captured in a single shot over large temporal windows.In addition to the decrease in acquisition time compared to standard multishot data acquisition,this technique allows measurements on systems far from equilibrium with large shotto-shot noise or with irreversible or poorly repeatable dynamics.Although DEOS has been demonstrated and verified for linearly polarized THz waveforms,we investigate the effects resulting from the presence of a secondary polarization component.This imposes new challenges for accurate waveform reconstruction,and opens the opportunity to measure out complex polarization states such as arbitrary elliptically polarized THz field.We demonstrate a single-shot diversity-electro-optic-sampling-based approach to capture both x-and y-THz fields simultaneously with a single(110)-cut EO crystal for THz polarimetry and ellipsometry over a wide range of frequencies.展开更多
The precise and timely extraction of railway signals is crucial for the creation of railway electronic maps.This paper introduces a novel real-time detection approach for dynamically adjusting railway signals,leveragi...The precise and timely extraction of railway signals is crucial for the creation of railway electronic maps.This paper introduces a novel real-time detection approach for dynamically adjusting railway signals,leveraging an enhanced Real-Time DEtection TRansformer(RT-DETR)model.The enhancement involves the integration of a vision Transformer with Dynamically Quantifiable Sampling Attention Mechanism(DQSAM)into the ResNet50 backbone of the RT-DETR framework,thereby enhancing the model’s efficiency and accuracy in handling intricate visual tasks.Secondly,an ultra-lightweight and effective Dynamic Grouping upSampler(DyGSample)is inserted into the efficient hybrid encode module as the up-sampling part.This operator can effectively upsample the feature graph without increasing the computational burden,and improve the model resolution and detail capture ability.In addition,in order to solve the problem of deep layer of model network and high operating cost,a new bounding box similarity loss function of rotation intersection over union based on minimum point distance is adopted in this paper,which takes into account all relevant factors of existing loss functions,namely overlapping or non-overlapping regions,center point distance,width and height deviation,and simplifies the calculation process.As a lightweight signal detection model with ultra-fast,high real-time,and high precision,the detection accuracy of this method is improved from 90.21%to 97.45%,which proves the superior performance and effectiveness of the improved real-time dynamic adjustment RT-DETR model in railway signal extraction.展开更多
基金supported by the National Natural Science Foundation of China [Grant agreement 31502124]the National Science and Technology Major Project of China [Grant agreement 2018ZX10733402]
文摘Objective Antimicrobial resistance(AMR)has become a global concern and is especially severe in China.To effectively and reliably provide AMR data,we developed a new high-throughput real-time PCR assay based on microfluidic dynamic technology,and screened multiple AMR genes in broiler fecal samples.Methods A high-throughput real-time PCR system with an new designed integrated fluidic circuit assay were performed AMR gene detection.A total of 273 broiler fecal samples collected from two geographically separated farms were screened AMR genes.Results The new assay with limits of detection ranging from 40.9 to 8,000 copies/reaction.The sensitivity rate,specificity rate,positive predictive value,negative predictive value and correct indices were 99.30%,98.08%,95.31%,99.79%,and 0.9755,respectively.Utilizing this assay,we demonstrate that AMR genes are widely spread,with positive detection rates ranging from 0 to 97.07%in 273 broiler fecal samples.bla CTX-M,bla TEM,mcr-1,fex A,cfr,optr A,and int I1 showed over 80%prevalence.The dissemination of AMR genes was distinct between the two farms.Conclusions We successfully established a new high-throughput real-time PCR assay applicable to AMR gene surveillance from fecal samples.The widespread existence of AMR genes detected in broiler farms highlights the current and severe problem of AMR.
文摘A new method jamming against Waveform Agile SAR/GMTI (Ground Moving Target Indication) is proposed, which is called the intermittent sampling motion scatter wave jamming. This jamming can form multi-false vivid targets scene taking the real moving targets’ all scattering information. The intermittent sampling repeater can also repeat in the waveform agile SAR current pulses. As a clever jamming mode, this jamming may realize electronic counter measure (ECM) against wave- form agile SAR. Detailed discussions about the jamming performance are given. The processing output of the jamming is derived theoretically. The superiority of this jamming is analyzed in theory. The theoretical feasibility and validity are proved by simulation experiments.
基金Supported by the National Natural Science Foundation of China(Nos.11175171,10835006,11105139 and 10975133)
文摘A digital three-detector positron lifetime spectrometer was developed.It consists of a DRS4 waveform digitizing board and three La Br3scintillation detectors coupled to XP2020Q photomultiplier tubes.DRS4 waveform digitizing allows data sampling at up to 5 GSPS with high amplitude resolution,with good time scale linearity and stability.In the triple-coincidence,the new system could reach a 195 ps time resolution,which is better than the conventional analog apparatus with the same detectors.This spectrometer can be applied to the other scintillation timing measurements with picoseconds accuracy.
文摘Flying plots detection has been the focus of relay protection in power system for a long time. With the promotion of Smart substation in our country, the number of SV devices is greatly increased. Abnormal data (flying plot) caused by sampling device itself has brought tremendous pressure to the power system. The traditional flying plot detection algorithm has plenty of defects, such as low pertinence, low sensitivity and long sampling period. This paper proposes a new algorithm to identify flying plot by analyzing the wave form characteristics of sampling data. The traditional waveform recognition methods are combined in this algorithm. It has the concept of standard wave window and can distinguish flying plot in a short time. In addition, sine recovery algorithm is used to recover the flying plot. This paper uses PSCAD software to verify the validity of this algorithm. Simulation results show that the proposed method has high reliability.
基金supported by the National Key Research and Development Program(2017YFC1500301)the National Natural Science Foundation of China(U1839206,91730306)
文摘Full waveform inversion(FWI) has been increasingly more and more important in seismology to better understand the interior structure of the Earth. FWI, by taking advantage of both the traveltime and amplitude in the data, provides high-resolution model parameters of the earth which can produce images with high resolution. However, this inversion method conventionally suffers from non-uniqueness due to many local minima of the objective function and large computing costs. In this study, we propose a new FWI method in a semi-random framework by integrating the ensemble Kalman filter and uniform sampling without replacement. Numerical results demonstrate that the new method can achieve highresolution results and a wider convergence domain. Accordingly, the new method overcomes the disadvantage of conventional FWIs that depend strongly on the initial model.
文摘针对间歇采样转发干扰产生的假目标和目标高速运动产生的多普勒频移导致雷达脉压性能急剧下降的问题,提出一种高多普勒容限的线性调频离散相位编码(linear frequency modulation-discrete phase coding,LFM-DPC)复合调制相干波形集设计方法。在一定多普勒频移范围内,以最小化未转发信号自模糊函数旁瓣能量以及未转发信号与转发信号互模糊函数能量建立优化模型,并设计一种基于KKT(Karush-Kuhn-Tucker)最优性条件的迭代算法对模型求解。仿真实验表明,相比于遗传算法和单一调制的LFM和DPC信号,基于KKT最优性条件的交替迭代优化算法优化的LFM-DPC波形集有更好的抗间歇采样转发干扰性能。
文摘With the recent development of diversity electro-optic sampling(DEOS),significant progress has been made in the range of applicability of single-shot EOS measurements,allowing broadband THz waveforms to be captured in a single shot over large temporal windows.In addition to the decrease in acquisition time compared to standard multishot data acquisition,this technique allows measurements on systems far from equilibrium with large shotto-shot noise or with irreversible or poorly repeatable dynamics.Although DEOS has been demonstrated and verified for linearly polarized THz waveforms,we investigate the effects resulting from the presence of a secondary polarization component.This imposes new challenges for accurate waveform reconstruction,and opens the opportunity to measure out complex polarization states such as arbitrary elliptically polarized THz field.We demonstrate a single-shot diversity-electro-optic-sampling-based approach to capture both x-and y-THz fields simultaneously with a single(110)-cut EO crystal for THz polarimetry and ellipsometry over a wide range of frequencies.
基金supported by the Hunan Provincial Natural Science Foundation of China(Nos.2025JJ70018 and 2025JJ70057)the Hunan Provincial Key Research and Development Program(No.2024JK2065).
文摘The precise and timely extraction of railway signals is crucial for the creation of railway electronic maps.This paper introduces a novel real-time detection approach for dynamically adjusting railway signals,leveraging an enhanced Real-Time DEtection TRansformer(RT-DETR)model.The enhancement involves the integration of a vision Transformer with Dynamically Quantifiable Sampling Attention Mechanism(DQSAM)into the ResNet50 backbone of the RT-DETR framework,thereby enhancing the model’s efficiency and accuracy in handling intricate visual tasks.Secondly,an ultra-lightweight and effective Dynamic Grouping upSampler(DyGSample)is inserted into the efficient hybrid encode module as the up-sampling part.This operator can effectively upsample the feature graph without increasing the computational burden,and improve the model resolution and detail capture ability.In addition,in order to solve the problem of deep layer of model network and high operating cost,a new bounding box similarity loss function of rotation intersection over union based on minimum point distance is adopted in this paper,which takes into account all relevant factors of existing loss functions,namely overlapping or non-overlapping regions,center point distance,width and height deviation,and simplifies the calculation process.As a lightweight signal detection model with ultra-fast,high real-time,and high precision,the detection accuracy of this method is improved from 90.21%to 97.45%,which proves the superior performance and effectiveness of the improved real-time dynamic adjustment RT-DETR model in railway signal extraction.
