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Scheduling transactions in mobile distributed real-time database systems 被引量:1
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作者 雷向东 赵跃龙 +1 位作者 陈松乔 袁晓莉 《Journal of Central South University of Technology》 EI 2008年第4期545-551,共7页
A DMVOCC-MVDA (distributed multiversion optimistic concurrency control with multiversion dynamic adjustment) protocol was presented to process mobile distributed real-time transaction in mobile broadcast environment... A DMVOCC-MVDA (distributed multiversion optimistic concurrency control with multiversion dynamic adjustment) protocol was presented to process mobile distributed real-time transaction in mobile broadcast environments. At the mobile hosts, all transactions perform local pre-validation. The local pre-validation process is carried out against the committed transactions at the server in the last broadcast cycle. Transactions that survive in local pre-validation must be submitted to the server for local final validation. The new protocol eliminates conflicts between mobile read-only and mobile update transactions, and resolves data conflicts flexibly by using multiversion dynamic adjustment of serialization order to avoid unnecessary restarts of transactions. Mobile read-only transactions can be committed with no-blocking, and respond time of mobile read-only transactions is greatly shortened. The tolerance of mobile transactions of disconnections from the broadcast channel is increased. In global validation mobile distributed transactions have to do check to ensure distributed serializability in all participants. The simulation results show that the new concurrency control protocol proposed offers better performance than other protocols in terms of miss rate, restart rate, commit rate. Under high work load (think time is ls) the miss rate of DMVOCC-MVDA is only 14.6%, is significantly lower than that of other protocols. The restart rate of DMVOCC-MVDA is only 32.3%, showing that DMVOCC-MVDA can effectively reduce the restart rate of mobile transactions. And the commit rate of DMVOCC-MVDA is up to 61.2%, which is obviously higher than that of other protocols. 展开更多
关键词 mobile distributed real-time database systems muliversion optimistic concurrency control multiversion dynamic adjustment pre-validation multiversion data broadcast
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A dynamic crash recovery scheme for distributed real-time database systems
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作者 肖迎元 刘云生 +2 位作者 刘小峰 廖国琼 王洪亚 《Journal of Shanghai University(English Edition)》 CAS 2006年第6期510-516,共7页
Recovery performance in the event of failures is very important for distributed real-time database systems. This paper presents a time-cognizant logging-based crash recovery scheme (TCLCRS) that aims at distributed ... Recovery performance in the event of failures is very important for distributed real-time database systems. This paper presents a time-cognizant logging-based crash recovery scheme (TCLCRS) that aims at distributed real-time databases, which adopts a main memory database as its ground support. In our scheme, each site maintains a real-time log for local transactions and the subtransactions, which execute at the site, and execte local checkpointing independently. Log records are stored in non-volatile high- speed store, which is divided into four different partitions based on transaction classes. During restart recovery after a site crash, partitioned crash recovery strategy is adopted to ensure that the site can be brought up before the entire local secondary database is reloaded in main memory. The partitioned crash recovery strategy not only guarantees the internal consistency to be recovered, but also guarantee the temporal consistency and recovery of the sates of physical world influenced by uncommitted transactions. Combined with two- phase commit protocol, TCLCRS can guarantee failure atomicity of distributed real-time transactions. 展开更多
关键词 distributed real-time database system partitioned real-time logging partitioned crash recovery.
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Quasi Serializable Concurrency Control in Distributed Real-Time Database Systems
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作者 党德鹏 Liu Yunsheng 《High Technology Letters》 EI CAS 2003年第1期72-76,共5页
This paper formally defines and analyses the new notion of correctness called quasi serializability, and then outlines corresponding concurrency control protocol QDHP for distributed real-time databases. Finally, thro... This paper formally defines and analyses the new notion of correctness called quasi serializability, and then outlines corresponding concurrency control protocol QDHP for distributed real-time databases. Finally, through a series of simulation studies, it shows that using the new concurrency control protocol the performance of distributed real-time databases can be much improved. 展开更多
关键词 distributed real-time database systems relaxing serializability real-tirne concurrency control
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Validation concurrency control protocol in parallel real-time database systems 被引量:3
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作者 LEI Xiang-dong(雷向东) YUAN Xiao-li(袁晓莉) 《Journal of Central South University of Technology》 2002年第3期197-201,共5页
In parallel real-time database systems, concurrency control protocols must satisfy time constraints as well as the integrity constraints. The authors present a validation concurrency control(VCC) protocol, which can e... In parallel real-time database systems, concurrency control protocols must satisfy time constraints as well as the integrity constraints. The authors present a validation concurrency control(VCC) protocol, which can enhance the performance of real-time concurrency control mechanism by reducing the number of transactions that might miss their deadlines, and compare the performance of validation concurrency control protocol with that of HP2PL(High priority two phase locking) protocol and OCC-TI-WAIT-50(Optimistic concurrency control-time interval-wait-50) protocol under shared-disk architecture by simulation. The simulation results reveal that the protocol the author presented can effectively reduce the number of transactions restarting which might miss their deadlines and performs better than HP2PL and OCC-TI-WAIT-50. It works well when arrival rate of transaction is lesser than threshold. However, due to resource contention the percentage of missing deadline increases sharply when arrival rate is greater than the threshold. 展开更多
关键词 PARALLEL database system real-time database CONCURRENCY control VALIDATION TRANSACTIONS
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Weak Serializable Concurrency Control in Distributed Real-Time Database Systems
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作者 党德鹏 刘云生 潘琳 《Journal of Shanghai University(English Edition)》 CAS 2002年第4期325-330,共6页
Most of the proposed concurrency control protocols for real time database systems are based on serializability theorem. Owing to the unique characteristics of real time database applications and the importance of sa... Most of the proposed concurrency control protocols for real time database systems are based on serializability theorem. Owing to the unique characteristics of real time database applications and the importance of satisfying the timing constraints of transactions, serializability is too strong as a correctness criterion and not suitable for real time databases in most cases. On the other hand, relaxed serializability including epsilon serializability and similarity serializability can allow more real time transactions to satisfy their timing constraints, but database consistency may be sacrificed to some extent. We thus propose the use of weak serializability(WSR) that is more relaxed than conflicting serializability while database consistency is maintained. In this paper, we first formally define the new notion of correctness called weak serializability. After the necessary and sufficient conditions for weak serializability are shown, corresponding concurrency control protocol WDHP(weak serializable distributed high priority protocol) is outlined for distributed real time databases, where a new lock mode called mask lock mode is proposed for simplifying the condition of global consistency. Finally, through a series of simulation studies, it is shown that using the new concurrency control protocol the performance of distributed real time databases can be greatly improved. 展开更多
关键词 distributed real time database systems relaxed serializability real time concurrency control read only transactions.
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Database of Eu^(2+)and Ce^(3+)Doped Phosphors for Development of Violet-light Excited White LEDs
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作者 ZHOU Zichun ZHANG Xiang +1 位作者 MING Chen SUN Yiyang 《无机材料学报》 北大核心 2026年第3期393-400,共8页
Commercial phosphor-converted white LEDs(pc-WLEDs)face two inherent limitations,namely blue light hazard and low color rendering index,due to the use of blue LEDs as excitation source.To address these challenges,viole... Commercial phosphor-converted white LEDs(pc-WLEDs)face two inherent limitations,namely blue light hazard and low color rendering index,due to the use of blue LEDs as excitation source.To address these challenges,violet LEDs are proposed as an alternative solution.Currently,phosphors that can be efficiently excited by violet light(with wavelengths from 400 to 420 nm)remain under development still.In this study,we utilize large language models to construct a comprehensive database of Eu^(2+)and Ce^(3+)doped phosphors for discovering novel violet-excited phosphors.A total of 822 phosphor data entries,including elemental compositions,crystal structures and excitation/emission wavelengths,have been extracted and validated from 9551 research papers.Compared with Ce^(3+)doped phosphors,the Eu^(2+)are in general more suited for violet-excited phosphors,as well as red-emitting phosphors.In particular,Eu^(2+)doped nitrides and sulfides are worth of exploration for violet-excited phosphors.This database is expected to be useful in the future development of phosphors for pc-WLEDs based on artificial intelligence methods.The datasets in this article are listed in Science Data Bank at http://doi.org/10.57760/sciencedb.34314. 展开更多
关键词 PHOSPHOR violet LED large language model database
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Electroacoustic tomography with dual-frequency array for real-time monitoring of electroporation
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作者 Luke Xu Yifei Xu Liangzhong Xiang 《Journal of Innovative Optical Health Sciences》 2026年第1期45-55,共11页
Electroacoustic Tomography(EAT)is an imaging technique that detects ultrasound waves induced by electrical pulses,offering a solution for real-time electroporation monitoring.This study presents EAT system using a dua... Electroacoustic Tomography(EAT)is an imaging technique that detects ultrasound waves induced by electrical pulses,offering a solution for real-time electroporation monitoring.This study presents EAT system using a dual-frequency ultrasound array.The broadband nature of electroacoustic signals requires ultrasound detector to cover both the high-frequency range(around 6MHz)signals generated by small targets and the low-frequency range(around 1MHz)signals generated by large targets.In our EAT system,we use the 6 MHz array to detect high-frequency signals from the electrodes,and the 1 MHz array for the electrical field.To test this,we conducted simulations using COMSOL Multiphysics^(®) and MATLAB's k-Wave toolbox,followed by experiments using a custom-built setup with a dual-frequency transducer and real-time data acquisition.The results demonstrated that the dual-frequency EAT system could accurately and simultaneously monitor the electroporation process,effectively showing both the treatment area and electrode placement with the application of 1 kV electric pulses with 100 ns duration.The axial resolution of the 6MHz array for EAT was 0.45 mm,significantly better than the 2mm resolution achieved with the 1MHz array.These findings validate the potential of dual-frequency EAT as a superior method for real-time electroporation monitoring. 展开更多
关键词 DUAL-FREQUENCY electroacoustic imaging real-time ELECTROPORATION
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Analysis of a dverse drug reactions a ssociated with ceftazidime/a vibactam using the FAERS database
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作者 Qiu-Lan Mo Juan-Juan Xie +1 位作者 Rong-Hui Wang Qiu-Gui Wei 《Medical Data Mining》 2026年第1期3-12,共10页
Background:The purpose of this study was to analyze and classify adverse drug events(ADEs)related to ceftazidime/avibactam reported in the Food and Drug Administration Adverse Event Reporting System(FAERS)database and... Background:The purpose of this study was to analyze and classify adverse drug events(ADEs)related to ceftazidime/avibactam reported in the Food and Drug Administration Adverse Event Reporting System(FAERS)database and to evaluate their potential safety signals since the drug’s market introduction.Methods:This analysis systematically extracted and filtered FAERS data for ceftazidime/avibactam from its market launch in 2015 to the last quarter of 2024,utilizing the Medical Dictionary for Regulatory Activities(MedDRA)terminology for ADE recoding.The analysis employed the reporting odds ratio(ROR)method to assess the strength of ADE signals and to identify significant diseases associated with infections,the hepatobiliary system,the urinary system,and the nervous system.Results:A review of 540 adverse reaction reports revealed significant signals of adverse effects related to infections,hepatobiliary disorders,urinary system issues,and neurological impairments,including pathogen resistance,liver and kidney function impairment,encephalopathy,thrombocytopenia,and toxic epidermal necrolysis.However,these issues require further clinical attention.Conclusion:Ceftazidime/avibactam is associated with a range of adverse reactions,necessitating enhanced clinical monitoring,particularly in patients with underlying liver or kidney dysfunction.Continuous risk assessment and vigilant monitoring are critical for its clinical use.However,this study is limited by inherent reporting biases and confounders associated with the spontaneous reporting database(FAERS).Future research should validate these signals through prospective cohort and mechanistic studies and explore personalized risk management strategies for high-risk populations. 展开更多
关键词 ceftazidime/avibactam FAERS database adverse drug event
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Block-Wise Sliding Recursive Wavelet Transform and Its Application in Real-Time Vehicle-Induced Signal Separation
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作者 Jie Li Nan An Youliang Ding 《Structural Durability & Health Monitoring》 2026年第1期1-22,共22页
Vehicle-induced response separation is a crucial issue in structural health monitoring(SHM).This paper proposes a block-wise sliding recursive wavelet transform algorithm to meet the real-time processing requirements ... Vehicle-induced response separation is a crucial issue in structural health monitoring(SHM).This paper proposes a block-wise sliding recursive wavelet transform algorithm to meet the real-time processing requirements of monitoring data.To extend the separation target from a fixed dataset to a continuously updating data stream,a block-wise sliding framework is first developed.This framework is further optimized considering the characteristics of real-time data streams,and its advantage in computational efficiency is theoretically demonstrated.During the decomposition and reconstruction processes,information from neighboring data blocks is fully utilized to reduce algorithmic complexity.In addition,a delay-setting strategy is introduced for each processing window to mitigate boundary effects,thereby balancing accuracy and efficiency.Simulated signal experiments are conducted to determine the optimal delay configuration and to verify the algorithm’s superior performance,achieving a lower Root Mean Square Error(RMSE)and only 0.0249 times the average computational time compared with the original algorithm.Furthermore,strain signals from the Lieshi River Bridge are employed to validate the method.The proposed algorithm successfully separates the static trend from vehicle-induced responses in real time across different sampling frequencies,demonstrating its effectiveness and applicability in real-time bridge monitoring. 展开更多
关键词 Wavelet transform vehicle-induced signal separation real-time structure monitoring
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Energy relief effect of real-time drilling to prevent rockburst in high-stress rock
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作者 Zhichao He Fengqiang Gong +2 位作者 Li Ren Weimin Yang Xuezhen Wu 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第2期1460-1475,共16页
To investigate the energy relief effect of real-time drilling in preventing rockburst in high-stress rock,a series of high-stress real-time drilling uniaxial compression tests were conducted on red sandstone specimens... To investigate the energy relief effect of real-time drilling in preventing rockburst in high-stress rock,a series of high-stress real-time drilling uniaxial compression tests were conducted on red sandstone specimens using the SG4500 drilling rig.Results showed that the mechanical behavior(i.e.peak strength and rockburst intensity)of the rock was weakened under high-stress real-time drilling and exhibited a downward trend as the drilling diameter increased.The real-time drilling energy dissipation index(ERD)was proposed to characterize the energy relief during high-stress real-time drilling.The ERD exhibited a linear increase with the real-time drilling diameter.Furthermore,the elastic strain energy of post-drilling rock showed a linear relationship with the square of stress across different stress levels,which also applied to the peak elastic strain energy and the square of peak stress.This findingreveals the intrinsic link between the weakening effect of peak elastic strain energy and peak strength due to high-stress real-time drilling,confirmingthe consistency between energy relief and pressure relief effects.By establishing relationships among rockburst proneness,peak elastic strain energy,and peak strength,it was demonstrated that high-stress real-time drilling reduces rockburst proneness through energy dissipation.Specifically,both peak elastic strain energy and rockburst proneness decreased with larger drill bit diameters,consistent with reductions in peak strength,rockburst intensity,and fractal dimensions of high-stress real-time drilled rock.These results validate the energy relief mechanism of real-time drilling in mitigating rockburst risks. 展开更多
关键词 Rock mechanics ROCKBURST real-time drilling Drilling energy relief Energy storage capacity Rockburst proneness
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Experimental study on real-time monitoring of surrounding rock 3D wave velocity structure and failure zone in deep tunnels
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作者 Hongyun Yang Chuandong Jiang +4 位作者 Yong Li Zhi Lin Xiang Wang Yifei Wu Wanlin Feng 《International Journal of Mining Science and Technology》 2026年第2期423-437,共15页
An innovative real-time monitoring method for surrounding rock damage based on microseismic time-lapse double-difference tomography is proposed for delayed dynamic damage identification and insufficient detection of a... An innovative real-time monitoring method for surrounding rock damage based on microseismic time-lapse double-difference tomography is proposed for delayed dynamic damage identification and insufficient detection of adverse geological conditions in deep-buried tunnel construction.The installation techniques for microseismic sensors were optimized by mounting sensors at bolt ends which significantly improves signal-to-noise ratio(SNR)and anti-interference capability compared to conventional borehole placement.Subsequently,a 3D wave velocity evolution model that incorporates construction-induced disturbances was established,enabling the first visualization of spatiotemporal variations in surrounding rock wave velocity.It finds significant wave velocity reduction near the tunnel face,with roof and floor damage zones extending 40–50 m;wave velocities approaching undisturbed levels at 15 m ahead of the working face and on the laterally undisturbed side;pronounced spatial asymmetry in wave velocity distribution—values on the left side exceed those on the right,with a clear stress concentration or transition zone located 10–15 m;and systematically lower velocities behind the face than in front,indicating asymmetric rock damage development.These results provide essential theoretical support and practical guidance for optimizing dynamic construction strategies,enabling real-time adjustment of support parameters,and establishing safety early warning systems in deep-buried tunnel engineering. 展开更多
关键词 Deep-buried tunnel Microseismic monitoring Wave velocity tomography Surrounding rock damage zone real-time monitoring
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A Hybrid Deep Learning Approach for Real-Time Cheating Behaviour Detection in Online Exams Using Video Captured Analysis
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作者 Dao Phuc Minh Huy Gia Nhu Nguyen Dac-Nhuong Le 《Computers, Materials & Continua》 2026年第3期1179-1198,共20页
Online examinations have become a dominant assessment mode,increasing concerns over academic integrity.To address the critical challenge of detecting cheating behaviours,this study proposes a hybrid deep learning appr... Online examinations have become a dominant assessment mode,increasing concerns over academic integrity.To address the critical challenge of detecting cheating behaviours,this study proposes a hybrid deep learning approach that combines visual detection and temporal behaviour classification.The methodology utilises object detection models—You Only Look Once(YOLOv12),Faster Region-based Convolutional Neural Network(RCNN),and Single Shot Detector(SSD)MobileNet—integrated with classification models such as Convolutional Neural Networks(CNN),Bidirectional Gated Recurrent Unit(Bi-GRU),and CNN-LSTM(Long Short-Term Memory).Two distinct datasets were used:the Online Exam Proctoring(EOP)dataset from Michigan State University and the School of Computer Science,Duy Tan Unievrsity(SCS-DTU)dataset collected in a controlled classroom setting.A diverse set of cheating behaviours,including book usage,unauthorised interaction,internet access,and mobile phone use,was categorised.Comprehensive experiments evaluated the models based on accuracy,precision,recall,training time,inference speed,and memory usage.We evaluate nine detector-classifier pairings under a unified budget and score them via a calibrated harmonic mean of detection and classification accuracies,enabling deployment-oriented selection under latency and memory constraints.Macro-Precision/Recall/F1 and Receiver Operating Characteristic-Area Under the Curve(ROC-AUC)are reported for the top configurations,revealing consistent advantages of object-centric pipelines for fine-grained cheating cues.The highest overall score is achieved by YOLOv12+CNN(97.15%accuracy),while SSD-MobileNet+CNN provides the best speed-efficiency trade-off for edge devices.This research provides valuable insights into selecting and deploying appropriate deep learning models for maintaining exam integrity under varying resource constraints. 展开更多
关键词 Online exam proctoring cheating behavior detection deep learning real-time monitoring object detection human behavior recognition
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Real-time decision support for bolter recovery safety:Long short-term memory network-driven aircraft sequencing
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作者 Wei Han Changjiu Li +4 位作者 Xichao Su Yong Zhang Fang Guo Tongtong Yu Xuan Li 《Defence Technology(防务技术)》 2026年第2期184-205,共22页
The highly dynamic nature,strong uncertainty,and coupled multiple safety constraints inherent in carrier aircraft recovery operations pose severe challenges for real-time decision-making.Addressing bolter scenarios,th... The highly dynamic nature,strong uncertainty,and coupled multiple safety constraints inherent in carrier aircraft recovery operations pose severe challenges for real-time decision-making.Addressing bolter scenarios,this study proposes an intelligent decision-making framework based on a deep long short-term memory Q-network.This framework transforms the real-time sequencing for bolter recovery problem into a partially observable Markov decision process.It employs a stacked long shortterm memory network to accurately capture the long-range temporal dependencies of bolter event chains and fuel consumption.Furthermore,it integrates a prioritized experience replay training mechanism to construct a safe and adaptive scheduling system capable of millisecond-level real-time decision-making.Experimental demonstrates that,within large-scale mass recovery scenarios,the framework achieves zero safety violations in static environments and maintains a fuel safety violation rate below 10%in dynamic scenarios,with single-step decision times at the millisecond level.The model exhibits strong generalization capability,effectively responding to unforeseen emergent situations—such as multiple bolters and fuel emergencies—without requiring retraining.This provides robust support for efficient carrier-based aircraft recovery operations. 展开更多
关键词 Carrier-based aircraft Recovery scheduling Deep reinforcement learning Long short-term memory networks Dynamic real-time decision-making
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An Efficient Crash Recovery Technique for Real-Time Main Memory Database 被引量:3
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作者 XIAOYing-yuan LIUYun-sheng LIAOGuo-qiong LIANGPing 《Wuhan University Journal of Natural Sciences》 CAS 2005年第1期61-64,共4页
This paper presents an efficient recovery scheme suitable for real-time mainmemory database. In the recovery scheme, log records are stored in non-volatile RAM which is dividedinto four different partitions based on t... This paper presents an efficient recovery scheme suitable for real-time mainmemory database. In the recovery scheme, log records are stored in non-volatile RAM which is dividedinto four different partitions based on transaction types. Similarly, a main memory database isdivided into four partitions based data types. When the using ratio of log store area exceeds thethreshold value, checkpoint procedure is triggered. During executing checkpoint procedure, someuseless log records are deleted. During restart recovery after a crash, partition reloading policyis adopted to assure that critical data are reloaded and restored in advance, so that the databasesystem can be brought up before the entire database is reloaded into main memory. Therefore downtime is obvionsly reduced. Simulation experiments show our recovery scheme obviously improves thesystem performance, and does a favor to meet the dtadlints of real-time transactions. 展开更多
关键词 real-time main memory database crash recovery log scheme
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The Multi-level Recovery of Main-memory Real-time Database Systems with ECBH
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作者 ZOU Yong-gui, GE Jun-wei (College of Computer Science and Technology, Chonqqing University of Posts and Telecommunications, Chongqing 400065, P.R. China) 《The Journal of China Universities of Posts and Telecommunications》 EI CSCD 2003年第1期15-24,共10页
Storing the whole database in the main-memory is a common method to processreal-time transaction in real-time database systems. The recovery mechanism of Main-memory Real-timeDatabase Systems (MMRTDBS) should reflect ... Storing the whole database in the main-memory is a common method to processreal-time transaction in real-time database systems. The recovery mechanism of Main-memory Real-timeDatabase Systems (MMRTDBS) should reflect the characteristics of the main-memory database andreal-time database because their structures are quite different from other conventional databasesystems. In this paper, therefore, we propose a multi-level recovery mechanism for main-memoryreal-time database systems with Extendable Chained Bucket Hashing (ECBH). Owing to the occurrence ofreal-time data in real-time systems, we should also consider it in our recovery mechanism .According to our performance test, this mechanism can improve the transaction concurrency, reducingtransactions ' deadline missing rate. 展开更多
关键词 DBMS multi-level recovery HASH main- memory database real-time
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基于Agricultural Animal Omics Database(AAOD)数据库挖掘绵羊GDF9基因变异
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作者 潘晔君 刘耿 +2 位作者 潘传英 张清峰 蓝贤勇 《畜牧兽医杂志》 2025年第3期1-9,共9页
目前利用大数据和组学数据库提高绵羊繁殖性能成为解决当前产业发展的关键之一。绵羊生长分化因子9(GDF9)基因对卵泡发育、排卵率及产羔数起着至关重要的作用,也是目前确定的绵羊多胎性状的主效基因之一;虽然其部分重要突变位点与产羔... 目前利用大数据和组学数据库提高绵羊繁殖性能成为解决当前产业发展的关键之一。绵羊生长分化因子9(GDF9)基因对卵泡发育、排卵率及产羔数起着至关重要的作用,也是目前确定的绵羊多胎性状的主效基因之一;虽然其部分重要突变位点与产羔数有关,但绵羊该基因所有可能基因变异如何挖掘仍然是一个尚待解决的问题。近年来,西北农林科技大学动物科技学院姜雨团队开发出Agricultural Animal Omics Database(AAOD)数据库,它整合了全球公共数据及中国本地特色绵羊品种的高深度基因组数据,涵盖50多个品种的遗传变异(SNPs、InDels)、表观标记及表型关联信息,并实现每月动态更新。基于此,本研究旨在利用AAOD数据库挖掘绵羊GDF9基因变异,并期望深入解析GDF9基因的分子结构、表达模式、富集分析、挖掘新的与繁殖力相关的突变位点及其在绵羊繁殖力中的潜在作用。这些结果将为基因编辑技术改良绵羊繁殖力提供新的潜在位点,将为提高绵羊繁殖力提供了遗传改良的理论依据与策略。 展开更多
关键词 绵羊 GDF9基因 Agricultural Animal Omics database(AAOD)数据库 单核苷酸多态性 插入缺失
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奶牛乳房炎病原体三重Real-time PCR检测方法的建立及应用
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作者 郭思宇 高雅欣 +5 位作者 纪佳豪 李梓豪 刘文扬 徐博 王三毛 李睿文 《动物医学进展》 北大核心 2025年第12期39-44,共6页
为了建立同时检测奶牛临床型乳房炎中肺炎克雷伯菌(Kp)、产色葡萄球菌(Sc)和牛支原体(Mb),基于Kp ZKIR基因、Sc sodA基因和Mb opp D/F基因设计特异性引物,建立三重实时定量荧光PCR方法(real-time PCR)。试验采用在单一real-time PCR检... 为了建立同时检测奶牛临床型乳房炎中肺炎克雷伯菌(Kp)、产色葡萄球菌(Sc)和牛支原体(Mb),基于Kp ZKIR基因、Sc sodA基因和Mb opp D/F基因设计特异性引物,建立三重实时定量荧光PCR方法(real-time PCR)。试验采用在单一real-time PCR检测方法的基础上对三重real-time PCR检测方法进行优化,并确定退火条件为60℃,肺炎克雷伯菌、产色葡萄球菌以及牛支原体上、下游引物浓度为20μmol/L、荧光探针浓度为10μmol/L。结果表明,该方法对标准品pUC57-ZKIR-Kp、pUC57-sodA-Sc、pUC57-opp D/F-Mb最低检测限分别为1.55×10^(2) copies/μL、1.44×10^(2) copies/μL、1.34×10^(2) copies/μL,灵敏度高;仅对Kp、Sc、Mb这3种病原产生荧光曲线,对其他病原无交叉反应,特异性强;其中组内、组间变异系数均小于2%,重复性良好。利用建立的多重real-time PCR对233份临床样品进行检测,Kp、Sc、Mb检出率分别为73.09%、21.97%、6.72%,与单一real-time PCR方法检测结果一致。说明建立的多重real-time PCR在实际应用中具有灵敏度高、特异性强、重复性良好、检测速度快等优点,可为奶牛临床型乳房炎病原的快速检测、临床诊断和流行病学调查提供有效检测手段。 展开更多
关键词 临床型乳房炎 三重real-time PCR 肺炎克雷伯菌 产色葡萄球菌 牛支原体
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Prospects for Construction New Metamorphic Rock Database in Big Data Epoch 被引量:1
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作者 Bo Liu Mingguo Zhai 《Journal of Earth Science》 2025年第2期450-459,共10页
Research into metamorphism plays a pivotal role in reconstructing the evolution of continent,particularly through the study of ancient rocks that are highly susceptible to metamorphic alterations due to multiple tecto... Research into metamorphism plays a pivotal role in reconstructing the evolution of continent,particularly through the study of ancient rocks that are highly susceptible to metamorphic alterations due to multiple tectonic activities.In the big data era,the establishment of new data platforms and the application of big data methods have become a focus for metamorphic rocks.Significant progress has been made in creating specialized databases,compiling comprehensive datasets,and utilizing data analytics to address complex scientific questions.However,many existing databases are inadequate in meeting the specific requirements of metamorphic research,resulting from a substantial amount of valuable data remaining uncollected.Therefore,constructing new databases that can cope with the development of the data era is necessary.This article provides an extensive review of existing databases related to metamorphic rocks and discusses data-driven studies in this.Accordingly,several crucial factors that need to be taken into consideration in the establishment of specialized metamorphic databases are identified,aiming to leverage data-driven applications to achieve broader scientific objectives in metamorphic research. 展开更多
关键词 metamorphic rock database big data data-driven research PETROLOGY
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Real-time monitoring of disc cutter wear in tunnel boring machines:A sound and vibration sensor-based approach with machine learning technique 被引量:1
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作者 Mohammad Amir Akhlaghi Raheb Bagherpour Seyed Hadi Hoseinie 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第3期1700-1722,共23页
Large portions of the tunnel boring machine(TBM)construction cost are attributed to disc cutter consumption,and assessing the disc cutter's wear level can help determine the optimal time to replace the disc cutter... Large portions of the tunnel boring machine(TBM)construction cost are attributed to disc cutter consumption,and assessing the disc cutter's wear level can help determine the optimal time to replace the disc cutter.Therefore,the need to monitor disc cutter wear in real-time has emerged as a technical challenge for TBMs.In this study,real-time disc cutter wear monitoring is developed based on sound and vibration sensors.For this purpose,the microphone and accelerometer were used to record the sound and vibration signals of cutting three different types of rocks with varying abrasions on a laboratory scale.The relationship between disc cutter wear and the sound and vibration signal was determined by comparing the measurements of disc cutter wear with the signal plots for each sample.The features extracted from the signals showed that the sound and vibration signals are impacted by the progression of disc wear during the rock-cutting process.The signal features obtained from the rock-cutting operation were utilized to verify the machine learning techniques.The results showed that the multilayer perceptron(MLP),random subspace-based decision tree(RS-DT),DT,and random forest(RF)methods could predict the wear level of the disc cutter with an accuracy of 0.89,0.951,0.951,and 0.927,respectively.Based on the accuracy of the models and the confusion matrix,it was found that the RS-DT model has the best estimate for predicting the level of disc wear.This research has developed a method that can potentially determine when to replace a tool and assess disc wear in real-time. 展开更多
关键词 TBM disc cutter WEAR SOUND VIBRATION Machine learning real-time wear estimation
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IoT-Based Real-Time Medical-Related Human Activity Recognition Using Skeletons and Multi-Stage Deep Learning for Healthcare 被引量:1
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作者 Subrata Kumer Paul Abu Saleh Musa Miah +3 位作者 Rakhi Rani Paul Md.EkramulHamid Jungpil Shin Md Abdur Rahim 《Computers, Materials & Continua》 2025年第8期2513-2530,共18页
The Internet of Things(IoT)and mobile technology have significantly transformed healthcare by enabling real-time monitoring and diagnosis of patients.Recognizing Medical-Related Human Activities(MRHA)is pivotal for he... The Internet of Things(IoT)and mobile technology have significantly transformed healthcare by enabling real-time monitoring and diagnosis of patients.Recognizing Medical-Related Human Activities(MRHA)is pivotal for healthcare systems,particularly for identifying actions critical to patient well-being.However,challenges such as high computational demands,low accuracy,and limited adaptability persist in Human Motion Recognition(HMR).While some studies have integrated HMR with IoT for real-time healthcare applications,limited research has focused on recognizing MRHA as essential for effective patient monitoring.This study proposes a novel HMR method tailored for MRHA detection,leveraging multi-stage deep learning techniques integrated with IoT.The approach employs EfficientNet to extract optimized spatial features from skeleton frame sequences using seven Mobile Inverted Bottleneck Convolutions(MBConv)blocks,followed by Convolutional Long Short Term Memory(ConvLSTM)to capture spatio-temporal patterns.A classification module with global average pooling,a fully connected layer,and a dropout layer generates the final predictions.The model is evaluated on the NTU RGB+D 120 and HMDB51 datasets,focusing on MRHA such as sneezing,falling,walking,sitting,etc.It achieves 94.85%accuracy for cross-subject evaluations and 96.45%for cross-view evaluations on NTU RGB+D 120,along with 89.22%accuracy on HMDB51.Additionally,the system integrates IoT capabilities using a Raspberry Pi and GSM module,delivering real-time alerts via Twilios SMS service to caregivers and patients.This scalable and efficient solution bridges the gap between HMR and IoT,advancing patient monitoring,improving healthcare outcomes,and reducing costs. 展开更多
关键词 real-time human motion recognition(HMR) ENConvLSTM EfficientNet ConvLSTM skeleton data NTU RGB+D 120 dataset MRHA
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