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Runtime environment for reflective real-time component 被引量:1
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作者 黄靖 卢炎生 《Journal of Shanghai University(English Edition)》 CAS 2008年第1期52-60,共9页
Reflective real-time component model is a special component model, which can identify timing constraint characteristics of component and support dynamic design-time amendment of real-time component according to users... Reflective real-time component model is a special component model, which can identify timing constraint characteristics of component and support dynamic design-time amendment of real-time component according to users' requirements. The reflective real-time component runtime environment is a bearing space and reflective infrastructure for this special component model. It consists of three parts and manages the lifecycle and various relevant services of reflective real-time component. In this paper its mechanism and relevant key techniques in design and realization are formally specified with the communicating sequential processing (CSP) and the extended timed communicating sequential processing (TCSP). Finally a prototype is established. Experimental study shows that this runtime environment can introduce a relevant reflective infrastructure guaranteeing dynamic and real-time features of software component. 展开更多
关键词 software component real-time runtime environment REFLECTION
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Cavity ring-down spectroscopy CO gas sensor integrating principal component analysis with savitzky-golay filtering
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作者 GUO Zi-long SHI Cheng-rui +4 位作者 DONG Yuan-yuan ZHANG Lei SUN Xiao-yuan SUN Jing-jing ZHOU Sheng 《中国光学(中英文)》 北大核心 2026年第1期179-189,共11页
The Savitzky-Golay(SG)filter,which employs polynomial least-squares approximations to smooth data and estimate derivatives,is widely used for processing noisy data.However,noise suppression by the SG filter is recogni... The Savitzky-Golay(SG)filter,which employs polynomial least-squares approximations to smooth data and estimate derivatives,is widely used for processing noisy data.However,noise suppression by the SG filter is recognized to be limited at data boundaries and high frequencies,which can significantly reduce the signal-to-noise ratio(SNR).To solve this problem,a novel method synergistically integrating Principal Component Analysis(PCA)with SG filtering is proposed in this paper.This approach avoids the is-sue of excessive smoothing associated with larger window sizes.The proposed PCA-SG filtering algorithm was applied to a CO gas sensing system based on Cavity Ring-Down Spectroscopy(CRDS).The perform-ance of the PCA-SG filtering algorithm is demonstrated through comparison with Moving Average Filtering(MAF),Wavelet Transformation(WT),Kalman Filtering(KF),and the SG filter.The results demonstrate that the proposed algorithm exhibits superior noise reduction capabilities compared to the other algorithms evaluated.The SNR of the ring-down signal was improved from 11.8612 dB to 29.0913 dB,and the stand-ard deviation of the extracted ring-down time constant was reduced from 0.037μs to 0.018μs.These results confirm that the proposed PCA-SG filtering algorithm effectively improves the smoothness of the ring-down curve data,demonstrating its feasibility. 展开更多
关键词 cavity ring-down spectroscopy CO gas sensor principal component analysis Savitzky-Golay filter
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Electroacoustic tomography with dual-frequency array for real-time monitoring of electroporation
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作者 Luke Xu Yifei Xu Liangzhong Xiang 《Journal of Innovative Optical Health Sciences》 2026年第1期45-55,共11页
Electroacoustic Tomography(EAT)is an imaging technique that detects ultrasound waves induced by electrical pulses,offering a solution for real-time electroporation monitoring.This study presents EAT system using a dua... Electroacoustic Tomography(EAT)is an imaging technique that detects ultrasound waves induced by electrical pulses,offering a solution for real-time electroporation monitoring.This study presents EAT system using a dual-frequency ultrasound array.The broadband nature of electroacoustic signals requires ultrasound detector to cover both the high-frequency range(around 6MHz)signals generated by small targets and the low-frequency range(around 1MHz)signals generated by large targets.In our EAT system,we use the 6 MHz array to detect high-frequency signals from the electrodes,and the 1 MHz array for the electrical field.To test this,we conducted simulations using COMSOL Multiphysics^(®) and MATLAB's k-Wave toolbox,followed by experiments using a custom-built setup with a dual-frequency transducer and real-time data acquisition.The results demonstrated that the dual-frequency EAT system could accurately and simultaneously monitor the electroporation process,effectively showing both the treatment area and electrode placement with the application of 1 kV electric pulses with 100 ns duration.The axial resolution of the 6MHz array for EAT was 0.45 mm,significantly better than the 2mm resolution achieved with the 1MHz array.These findings validate the potential of dual-frequency EAT as a superior method for real-time electroporation monitoring. 展开更多
关键词 DUAL-FREQUENCY electroacoustic imaging real-time ELECTROPORATION
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Study on Active Components from the Stems of Polyalthia plagioneura(II)
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作者 Bingjing LIU Guikun TANG 《Agricultural Biotechnology》 2026年第1期63-64,69,共3页
[Objectives]This study was conducted to isolate and identify the components from stems of Polyalthia plagioneura.[Methods]The compounds were isolated and purified by silica gel column,Sephadex LH-20,and C_(18) chromat... [Objectives]This study was conducted to isolate and identify the components from stems of Polyalthia plagioneura.[Methods]The compounds were isolated and purified by silica gel column,Sephadex LH-20,and C_(18) chromatography.Their chemical structures were elucidated on the basis of physicochemical properties and spectral data.[Results]Five compounds were isolated and identified as:di(2-ethylhexyl)phthalate(1),cinnamic anhydride(2),phthalic acid(3),citric acid(4),and syringaldehyde(5).[Conclusions]All compounds were isolated from this plant for the first time. 展开更多
关键词 Polyalthia plagioneura Chemical component STEROID
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Block-Wise Sliding Recursive Wavelet Transform and Its Application in Real-Time Vehicle-Induced Signal Separation
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作者 Jie Li Nan An Youliang Ding 《Structural Durability & Health Monitoring》 2026年第1期1-22,共22页
Vehicle-induced response separation is a crucial issue in structural health monitoring(SHM).This paper proposes a block-wise sliding recursive wavelet transform algorithm to meet the real-time processing requirements ... Vehicle-induced response separation is a crucial issue in structural health monitoring(SHM).This paper proposes a block-wise sliding recursive wavelet transform algorithm to meet the real-time processing requirements of monitoring data.To extend the separation target from a fixed dataset to a continuously updating data stream,a block-wise sliding framework is first developed.This framework is further optimized considering the characteristics of real-time data streams,and its advantage in computational efficiency is theoretically demonstrated.During the decomposition and reconstruction processes,information from neighboring data blocks is fully utilized to reduce algorithmic complexity.In addition,a delay-setting strategy is introduced for each processing window to mitigate boundary effects,thereby balancing accuracy and efficiency.Simulated signal experiments are conducted to determine the optimal delay configuration and to verify the algorithm’s superior performance,achieving a lower Root Mean Square Error(RMSE)and only 0.0249 times the average computational time compared with the original algorithm.Furthermore,strain signals from the Lieshi River Bridge are employed to validate the method.The proposed algorithm successfully separates the static trend from vehicle-induced responses in real time across different sampling frequencies,demonstrating its effectiveness and applicability in real-time bridge monitoring. 展开更多
关键词 Wavelet transform vehicle-induced signal separation real-time structure monitoring
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Microstructure and properties of HPDC AM60 based alloys for super-sized integrated automotive components
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作者 Jing WANG Xin GUO +8 位作者 Jiang-feng SONG Dang-he SHI He-cong XIE Chun-yu LI Heng-rui HU Gong CHEN Bin JIANG Dong-xia XIANG Fu-sheng PAN 《Transactions of Nonferrous Metals Society of China》 2026年第2期433-450,共18页
The commercial AM60(Mg−6Al−0.3Mn)die-casting alloy was modified through Mn,Ce,and La micro-alloying,each at a content below 0.2 wt.%.SEM,TEM,and Micro-CT were employed to characterize the microstructures and propertie... The commercial AM60(Mg−6Al−0.3Mn)die-casting alloy was modified through Mn,Ce,and La micro-alloying,each at a content below 0.2 wt.%.SEM,TEM,and Micro-CT were employed to characterize the microstructures and properties of AM60 based alloys.AM60-0.2La alloy showed excellent mechanical properties.The ultimate tensile strength,yield strength,and elongation of(288.0±1.7)MPa,(158.0±1.0)MPa,and(22.0±3.0)%were achieved in AM60-0.2La alloy.Besides,AM60-0.2La alloy exhibited the best corrosion resistance(0.29 mm/a)and fluidity among the investigated four alloys.The excellent mechanical properties and corrosion resistance are mainly attributed to the grain refinement strengthening,low porosity,and low content of large shrinkage porosity,promising for super-sized integrated automotive components. 展开更多
关键词 magnesium alloys microstructure mechanical properties high-pressure die-casting integrated automotive components
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Comprehensive quality evaluation and principal component analysis of Crassostrea gigas from North Yellow Sea,China:characterizing nutritional,flavor,and functional profiles
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作者 Shanqin HUO Jixing PENG +3 位作者 Xinnan ZHAO Yichen LIN Xudong LI Zhijun TAN 《Journal of Oceanology and Limnology》 2026年第1期406-418,共13页
Crassostrea gigas has good taste and high nutritional value;however,there are few assessments of comprehensive and panoramic analyses of the nutritional quality of the northern oyster.To study the nutritional characte... Crassostrea gigas has good taste and high nutritional value;however,there are few assessments of comprehensive and panoramic analyses of the nutritional quality of the northern oyster.To study the nutritional characteristics of C.gigas from different sources(ploidy,region,size,and culture mode),C.gigas from various ploidy(diploid and triploid),regions(Rushan,Off-site fattening,and Rongcheng),sizes(small,medium,and large)and culture modes(nearshore and offshore)were selected for comparative analyses.The nutritional components(moisture,protein,fat,and mineral),flavor substances(taste amino acids,nucleotides,and succinic acid),and functional indices(eicosapentaenoic acid(EPA),docosahexaenoic acid(DHA),and taurine)of C.gigas were determined.Principal component analysis(PCA)was used to comprehensively evaluate the oysters and investigate the variations in nutritional quality.The PCA results indicate that protein,essential fatty acids,selenium,zinc,taste amino acids,taurine,EPA,and DHA were core components contributing to 82.25%of the cumulative variance,providing a more comprehensive reflection of the nutrient composition of C.gigas.The extensive quality rankings for the C.gigas were as follows:diploid>triploid,Rushan>fattening>Rongcheng,medium>large>small,and offshore>nearshore.The score rank revealed that diploid oysters of medium-size from Rushan demonstrated superior nutritional quality compared to other tested samples.This is the first comprehensive and systematic investigation of C.gigas in northern China to reveal the feature of nutrients,flavor,and functional components.The study provided data support for the culture,consumption,processing,research,and nutritional quality improvement of oyster industry. 展开更多
关键词 Crassostrea gigas nutritional quality principal component analysis comprehensive evaluation
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Multi-pass intermittent local loading process of large-scale rib-web component:Forming characteristics and implementing apparatus
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作者 Dawei ZHANG Peng DONG +2 位作者 Jingxiang LI Zijian YU Shengdun ZHAO 《Chinese Journal of Aeronautics》 2026年第1期601-625,共25页
The multi-pass intermittent local loading process,which features a more flexible processing path,can further enhance the second material distribution during local loading,improve the formability of components,and redu... The multi-pass intermittent local loading process,which features a more flexible processing path,can further enhance the second material distribution during local loading,improve the formability of components,and reduce forming loads.However,the absence of compatible forming equipment makes it difficult to control the constraint in the unloaded zones during the forming process.This difficulty complicates coordination and control of deformation,particularly for asymmetric rib-web components.Additionally,the current implementation involves multi-fire heating,a long process flow,and high energy consumption,which limits the popularization and application of the local loading process.In this study,a new multi-pass local loading hydraulic forming apparatus that can quickly and reliably switch between heavy-load deformation and low-load constraint for different local loading sub-dies was developed.A 10-tonne laboratory prototype was developed,and the forming characteristics during the forming process as well as the response characteristics of the hydraulic system during the multi-pass intermittent local loading of rib-web component were investigated using numerical simulations and physical experiments.Results indicated that,compared to a whole loading process with the same initial geometry of billet,the total forming load(i.e.,the sum of loaded and restrained loads)is reduced by more than 40%with the local loading process,and by nearly 50%with multi-pass local loading.The multi-pass local loading process allows for more effective control of material flow compared to single-pass local loading,leading to improved cavity filling and reduced flow line disturbance.For a large-scale,complex titanium alloy bulkhead,the cavity filling problem was addressed by optimizing the multi-pass local loading path with an unequal thickness billet.The dynamic performance of the multi-pass local loading hydraulic system was found to be robust,with stable pressure transitions during motion and load switching for the sub-die(s).The dynamic characteristic of the hydraulic cylinder when switching from non-moving/unloaded state to a moving/loading state are consistent whether a load is present or not.However,the dynamic characteristics differ when switching from a moving/loading state to non-moving/unloaded state,showing opposite behavior.The developed hydraulic drive mechanism provides a way for implementation of multi-pass local loading without auxiliary operation and extra heating.The results of the study provide a foundation for the industrial production of large-scale,complex components with reduced force requirement and low-energy consumption. 展开更多
关键词 Forming characteristics Hydraulic system Intermittent local loading process Material flow Rib-web component
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Two-Stage LightGBM Framework for Cost-Sensitive Prediction of Impending Failures of Component X in Scania Trucks
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作者 Si-Woo Kim Yong Soo Kim 《Computers, Materials & Continua》 2026年第3期1222-1240,共19页
Predictive maintenance(PdM)is vital for ensuring the reliability,safety,and cost efficiency of heavyduty vehicle fleets.However,real-world sensor data are often highly imbalanced,noisy,and temporally irregular,posing ... Predictive maintenance(PdM)is vital for ensuring the reliability,safety,and cost efficiency of heavyduty vehicle fleets.However,real-world sensor data are often highly imbalanced,noisy,and temporally irregular,posing significant challenges to model robustness and deployment.Using multivariate time-series data from Scania trucks,this study proposes a novel PdM framework that integrates efficient feature summarization with cost-sensitive hierarchical classification.First,the proposed last_k_summary method transforms recent operational records into compact statistical and trend-based descriptors while preserving missingness,allowing LightGBM to leverage its inherent split rules without ad-hoc imputation.Then,a two-stage LightGBM framework is developed for fault detection and severity classification:Stage A performs safety-prioritized fault screening(normal vs.fault)with a false-negativeweighted objective,and Stage B refines the detected faults into four severity levels through a cascaded hierarchy of binary classifiers.Under the official cost matrix of the IDA Industrial Challenge,the framework achieves total misclassification costs of 36,113(validation)and 36,314(test),outperforming XGBoost and Bi-LSTM by 3.8%-13.5%while maintaining high recall for the safety-critical class(0.83 validation,0.77 test).These results demonstrate that the proposed approach not only improves predictive accuracy but also provides a practical and deployable PdM solution that reduces maintenance cost,enhances fleet safety,and supports data-driven decision-making in industrial environments. 展开更多
关键词 Predictive maintenance two-stage classification cost-based evaluation LightGBM scania component X dataset
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Energy relief effect of real-time drilling to prevent rockburst in high-stress rock
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作者 Zhichao He Fengqiang Gong +2 位作者 Li Ren Weimin Yang Xuezhen Wu 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第2期1460-1475,共16页
To investigate the energy relief effect of real-time drilling in preventing rockburst in high-stress rock,a series of high-stress real-time drilling uniaxial compression tests were conducted on red sandstone specimens... To investigate the energy relief effect of real-time drilling in preventing rockburst in high-stress rock,a series of high-stress real-time drilling uniaxial compression tests were conducted on red sandstone specimens using the SG4500 drilling rig.Results showed that the mechanical behavior(i.e.peak strength and rockburst intensity)of the rock was weakened under high-stress real-time drilling and exhibited a downward trend as the drilling diameter increased.The real-time drilling energy dissipation index(ERD)was proposed to characterize the energy relief during high-stress real-time drilling.The ERD exhibited a linear increase with the real-time drilling diameter.Furthermore,the elastic strain energy of post-drilling rock showed a linear relationship with the square of stress across different stress levels,which also applied to the peak elastic strain energy and the square of peak stress.This findingreveals the intrinsic link between the weakening effect of peak elastic strain energy and peak strength due to high-stress real-time drilling,confirmingthe consistency between energy relief and pressure relief effects.By establishing relationships among rockburst proneness,peak elastic strain energy,and peak strength,it was demonstrated that high-stress real-time drilling reduces rockburst proneness through energy dissipation.Specifically,both peak elastic strain energy and rockburst proneness decreased with larger drill bit diameters,consistent with reductions in peak strength,rockburst intensity,and fractal dimensions of high-stress real-time drilled rock.These results validate the energy relief mechanism of real-time drilling in mitigating rockburst risks. 展开更多
关键词 Rock mechanics ROCKBURST real-time drilling Drilling energy relief Energy storage capacity Rockburst proneness
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Experimental study on real-time monitoring of surrounding rock 3D wave velocity structure and failure zone in deep tunnels
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作者 Hongyun Yang Chuandong Jiang +4 位作者 Yong Li Zhi Lin Xiang Wang Yifei Wu Wanlin Feng 《International Journal of Mining Science and Technology》 2026年第2期423-437,共15页
An innovative real-time monitoring method for surrounding rock damage based on microseismic time-lapse double-difference tomography is proposed for delayed dynamic damage identification and insufficient detection of a... An innovative real-time monitoring method for surrounding rock damage based on microseismic time-lapse double-difference tomography is proposed for delayed dynamic damage identification and insufficient detection of adverse geological conditions in deep-buried tunnel construction.The installation techniques for microseismic sensors were optimized by mounting sensors at bolt ends which significantly improves signal-to-noise ratio(SNR)and anti-interference capability compared to conventional borehole placement.Subsequently,a 3D wave velocity evolution model that incorporates construction-induced disturbances was established,enabling the first visualization of spatiotemporal variations in surrounding rock wave velocity.It finds significant wave velocity reduction near the tunnel face,with roof and floor damage zones extending 40–50 m;wave velocities approaching undisturbed levels at 15 m ahead of the working face and on the laterally undisturbed side;pronounced spatial asymmetry in wave velocity distribution—values on the left side exceed those on the right,with a clear stress concentration or transition zone located 10–15 m;and systematically lower velocities behind the face than in front,indicating asymmetric rock damage development.These results provide essential theoretical support and practical guidance for optimizing dynamic construction strategies,enabling real-time adjustment of support parameters,and establishing safety early warning systems in deep-buried tunnel engineering. 展开更多
关键词 Deep-buried tunnel Microseismic monitoring Wave velocity tomography Surrounding rock damage zone real-time monitoring
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A Hybrid Deep Learning Approach for Real-Time Cheating Behaviour Detection in Online Exams Using Video Captured Analysis
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作者 Dao Phuc Minh Huy Gia Nhu Nguyen Dac-Nhuong Le 《Computers, Materials & Continua》 2026年第3期1179-1198,共20页
Online examinations have become a dominant assessment mode,increasing concerns over academic integrity.To address the critical challenge of detecting cheating behaviours,this study proposes a hybrid deep learning appr... Online examinations have become a dominant assessment mode,increasing concerns over academic integrity.To address the critical challenge of detecting cheating behaviours,this study proposes a hybrid deep learning approach that combines visual detection and temporal behaviour classification.The methodology utilises object detection models—You Only Look Once(YOLOv12),Faster Region-based Convolutional Neural Network(RCNN),and Single Shot Detector(SSD)MobileNet—integrated with classification models such as Convolutional Neural Networks(CNN),Bidirectional Gated Recurrent Unit(Bi-GRU),and CNN-LSTM(Long Short-Term Memory).Two distinct datasets were used:the Online Exam Proctoring(EOP)dataset from Michigan State University and the School of Computer Science,Duy Tan Unievrsity(SCS-DTU)dataset collected in a controlled classroom setting.A diverse set of cheating behaviours,including book usage,unauthorised interaction,internet access,and mobile phone use,was categorised.Comprehensive experiments evaluated the models based on accuracy,precision,recall,training time,inference speed,and memory usage.We evaluate nine detector-classifier pairings under a unified budget and score them via a calibrated harmonic mean of detection and classification accuracies,enabling deployment-oriented selection under latency and memory constraints.Macro-Precision/Recall/F1 and Receiver Operating Characteristic-Area Under the Curve(ROC-AUC)are reported for the top configurations,revealing consistent advantages of object-centric pipelines for fine-grained cheating cues.The highest overall score is achieved by YOLOv12+CNN(97.15%accuracy),while SSD-MobileNet+CNN provides the best speed-efficiency trade-off for edge devices.This research provides valuable insights into selecting and deploying appropriate deep learning models for maintaining exam integrity under varying resource constraints. 展开更多
关键词 Online exam proctoring cheating behavior detection deep learning real-time monitoring object detection human behavior recognition
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Real-time decision support for bolter recovery safety:Long short-term memory network-driven aircraft sequencing
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作者 Wei Han Changjiu Li +4 位作者 Xichao Su Yong Zhang Fang Guo Tongtong Yu Xuan Li 《Defence Technology(防务技术)》 2026年第2期184-205,共22页
The highly dynamic nature,strong uncertainty,and coupled multiple safety constraints inherent in carrier aircraft recovery operations pose severe challenges for real-time decision-making.Addressing bolter scenarios,th... The highly dynamic nature,strong uncertainty,and coupled multiple safety constraints inherent in carrier aircraft recovery operations pose severe challenges for real-time decision-making.Addressing bolter scenarios,this study proposes an intelligent decision-making framework based on a deep long short-term memory Q-network.This framework transforms the real-time sequencing for bolter recovery problem into a partially observable Markov decision process.It employs a stacked long shortterm memory network to accurately capture the long-range temporal dependencies of bolter event chains and fuel consumption.Furthermore,it integrates a prioritized experience replay training mechanism to construct a safe and adaptive scheduling system capable of millisecond-level real-time decision-making.Experimental demonstrates that,within large-scale mass recovery scenarios,the framework achieves zero safety violations in static environments and maintains a fuel safety violation rate below 10%in dynamic scenarios,with single-step decision times at the millisecond level.The model exhibits strong generalization capability,effectively responding to unforeseen emergent situations—such as multiple bolters and fuel emergencies—without requiring retraining.This provides robust support for efficient carrier-based aircraft recovery operations. 展开更多
关键词 Carrier-based aircraft Recovery scheduling Deep reinforcement learning Long short-term memory networks Dynamic real-time decision-making
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Real-time lane departure warning system based on principal component analysis of grayscale distribution and risk evaluation model 被引量:4
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作者 张伟伟 宋晓琳 张桂香 《Journal of Central South University》 SCIE EI CAS 2014年第4期1633-1642,共10页
A technology for unintended lane departure warning was proposed. As crucial information, lane boundaries were detected based on principal component analysis of grayscale distribution in search bars of given number and... A technology for unintended lane departure warning was proposed. As crucial information, lane boundaries were detected based on principal component analysis of grayscale distribution in search bars of given number and then each search bar was tracked using Kalman filter between frames. The lane detection performance was evaluated and demonstrated in ways of receiver operating characteristic, dice similarity coefficient and real-time performance. For lane departure detection, a lane departure risk evaluation model based on lasting time and frequency was effectively executed on the ARM-based platform. Experimental results indicate that the algorithm generates satisfactory lane detection results under different traffic and lighting conditions, and the proposed warning mechanism sends effective warning signals, avoiding most false warning. 展开更多
关键词 lane departure warning system lane detection lane tracking principal component analysis risk evaluation model ARM-based real-time system
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奶牛乳房炎病原体三重Real-time PCR检测方法的建立及应用
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作者 郭思宇 高雅欣 +5 位作者 纪佳豪 李梓豪 刘文扬 徐博 王三毛 李睿文 《动物医学进展》 北大核心 2025年第12期39-44,共6页
为了建立同时检测奶牛临床型乳房炎中肺炎克雷伯菌(Kp)、产色葡萄球菌(Sc)和牛支原体(Mb),基于Kp ZKIR基因、Sc sodA基因和Mb opp D/F基因设计特异性引物,建立三重实时定量荧光PCR方法(real-time PCR)。试验采用在单一real-time PCR检... 为了建立同时检测奶牛临床型乳房炎中肺炎克雷伯菌(Kp)、产色葡萄球菌(Sc)和牛支原体(Mb),基于Kp ZKIR基因、Sc sodA基因和Mb opp D/F基因设计特异性引物,建立三重实时定量荧光PCR方法(real-time PCR)。试验采用在单一real-time PCR检测方法的基础上对三重real-time PCR检测方法进行优化,并确定退火条件为60℃,肺炎克雷伯菌、产色葡萄球菌以及牛支原体上、下游引物浓度为20μmol/L、荧光探针浓度为10μmol/L。结果表明,该方法对标准品pUC57-ZKIR-Kp、pUC57-sodA-Sc、pUC57-opp D/F-Mb最低检测限分别为1.55×10^(2) copies/μL、1.44×10^(2) copies/μL、1.34×10^(2) copies/μL,灵敏度高;仅对Kp、Sc、Mb这3种病原产生荧光曲线,对其他病原无交叉反应,特异性强;其中组内、组间变异系数均小于2%,重复性良好。利用建立的多重real-time PCR对233份临床样品进行检测,Kp、Sc、Mb检出率分别为73.09%、21.97%、6.72%,与单一real-time PCR方法检测结果一致。说明建立的多重real-time PCR在实际应用中具有灵敏度高、特异性强、重复性良好、检测速度快等优点,可为奶牛临床型乳房炎病原的快速检测、临床诊断和流行病学调查提供有效检测手段。 展开更多
关键词 临床型乳房炎 三重real-time PCR 肺炎克雷伯菌 产色葡萄球菌 牛支原体
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Therapeutic components of acupuncture stimulation based on characteristics of sensory nerve and nervous signaling pathway 被引量:1
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作者 Hyo-Sun Wie Seung-Nam Kim 《Journal of Integrative Medicine》 2025年第2期106-112,共7页
Acupuncture,a therapeutic practice rooted in traditional Chinese medicine and integrated with modern neuroscience,achieves its effects by stimulating sensory nerves at specific anatomical points known as acupoints.Thi... Acupuncture,a therapeutic practice rooted in traditional Chinese medicine and integrated with modern neuroscience,achieves its effects by stimulating sensory nerves at specific anatomical points known as acupoints.This review systematically explores the therapeutic components of acupuncture,emphasizing the interplay between sensory nerve characteristics and neural signaling pathways.Key factors such as acupoint location,needling depth,stimulation intensity,retention time,and the induction of sensations(e.g.,Deqi)are analyzed for their roles in neural activation and clinical outcomes.The review highlights how variations in spinal segment targeting,tissue-specific receptor activation,and stimulation modalities(e.g.,manual acupuncture,electroacupuncture,moxibustion)influence therapeutic effects.Emerging evidence underscores the significance of ion channels,dermatomes,myotomes,and genespecific pathways in mediating systemic effects.Additionally,the differential roles of mechanical,thermal and nociceptive stimuli and the temporal dynamics of sensory and immune responses are addressed.While insights from animal models have advanced our understanding,their translation to clinical practice requires further investigation.This comprehensive review identifies critical parameters for optimizing acupuncture therapy,advocating for individualized treatment strategies informed by neuroanatomical and neurophysiological principles,ultimately enhancing its precision and efficacy in modern medicine. 展开更多
关键词 Sensory nerve ACUPUNCTURE Nervous system Therapeutic component Acupuncture point MERIDIANS
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Real-time monitoring of disc cutter wear in tunnel boring machines:A sound and vibration sensor-based approach with machine learning technique 被引量:1
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作者 Mohammad Amir Akhlaghi Raheb Bagherpour Seyed Hadi Hoseinie 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第3期1700-1722,共23页
Large portions of the tunnel boring machine(TBM)construction cost are attributed to disc cutter consumption,and assessing the disc cutter's wear level can help determine the optimal time to replace the disc cutter... Large portions of the tunnel boring machine(TBM)construction cost are attributed to disc cutter consumption,and assessing the disc cutter's wear level can help determine the optimal time to replace the disc cutter.Therefore,the need to monitor disc cutter wear in real-time has emerged as a technical challenge for TBMs.In this study,real-time disc cutter wear monitoring is developed based on sound and vibration sensors.For this purpose,the microphone and accelerometer were used to record the sound and vibration signals of cutting three different types of rocks with varying abrasions on a laboratory scale.The relationship between disc cutter wear and the sound and vibration signal was determined by comparing the measurements of disc cutter wear with the signal plots for each sample.The features extracted from the signals showed that the sound and vibration signals are impacted by the progression of disc wear during the rock-cutting process.The signal features obtained from the rock-cutting operation were utilized to verify the machine learning techniques.The results showed that the multilayer perceptron(MLP),random subspace-based decision tree(RS-DT),DT,and random forest(RF)methods could predict the wear level of the disc cutter with an accuracy of 0.89,0.951,0.951,and 0.927,respectively.Based on the accuracy of the models and the confusion matrix,it was found that the RS-DT model has the best estimate for predicting the level of disc wear.This research has developed a method that can potentially determine when to replace a tool and assess disc wear in real-time. 展开更多
关键词 TBM disc cutter WEAR SOUND VIBRATION Machine learning real-time wear estimation
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Research on multi-scale simulation and dynamic verification of high dynamic MEMS components in additive manufacturing 被引量:1
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作者 Sining Lv Hengzhen Feng +2 位作者 Wenzhong Lou Chuan Xiao Shiyi Li 《Defence Technology(防务技术)》 2025年第5期275-291,共17页
Metal Additive Manufacturing(MAM) technology has become an important means of rapid prototyping precision manufacturing of special high dynamic heterogeneous complex parts. In response to the micromechanical defects s... Metal Additive Manufacturing(MAM) technology has become an important means of rapid prototyping precision manufacturing of special high dynamic heterogeneous complex parts. In response to the micromechanical defects such as porosity issues, significant deformation, surface cracks, and challenging control of surface morphology encountered during the selective laser melting(SLM) additive manufacturing(AM) process of specialized Micro Electromechanical System(MEMS) components, multiparameter optimization and micro powder melt pool/macro-scale mechanical properties control simulation of specialized components are conducted. The optimal parameters obtained through highprecision preparation and machining of components and static/high dynamic verification are: laser power of 110 W, laser speed of 600 mm/s, laser diameter of 75 μm, and scanning spacing of 50 μm. The density of the subordinate components under this reference can reach 99.15%, the surface hardness can reach 51.9 HRA, the yield strength can reach 550 MPa, the maximum machining error of the components is 4.73%, and the average surface roughness is 0.45 μm. Through dynamic hammering and high dynamic firing verification, SLM components meet the requirements for overload resistance. The results have proven that MEM technology can provide a new means for the processing of MEMS components applied in high dynamic environments. The parameters obtained in the conclusion can provide a design basis for the additive preparation of MEMS components. 展开更多
关键词 Additive manufacturing High dynamic MEMS components Multiscale control Process optimization High dynamic verification
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Simultaneous identification of multiple animal-derived components in meat and meat products by using MNP marker based on high-throughput sequencing 被引量:1
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作者 Yan Yi Zhanyue Jiang +9 位作者 Lixia Ma Xiaoni Hou Lun Li Deping Ye Juanlan Du Hai Peng Guoquan Han Huaiping Li Jiangwen Tang Lihua Zhou 《Food Science and Human Wellness》 2025年第4期1566-1575,共10页
In order to save manpower and time costs,and to achieve simultaneous detection of multiple animal-derived components in meat and meat products,this study used multiple nucleotide polymorphism(MNP)marker technology bas... In order to save manpower and time costs,and to achieve simultaneous detection of multiple animal-derived components in meat and meat products,this study used multiple nucleotide polymorphism(MNP)marker technology based on the principle of high-throughput sequencing,and established a multi-locus 10 animalderived components identification method of cattle,goat,sheep,donkey,horse,chicken,duck,goose,pigeon,quail in meat and meat products.The specific loci of each species could be detected and the species could be accurately identified,including 5 loci for cattle and duck,3 loci for sheep,9 loci for chicken and horse,10 loci for goose and pigeon,6 loci for quail and 1 locus for donkey and goat,and an adulteration model was established to simulate commercially available samples.The results showed that the method established in this study had high throughput,good repeatability and accuracy,and was able to identify 10 animalderived components simultaneously with 100%repeatability accuracy.The detection limit was 0.1%(m/m)in simulated samples of chicken,duck and horse.Using the method established in this study to test commercially available samples,4 samples from 14 commercially available samples were detected to be inconsistent with the labels,of which 2 did not contain the target ingredient and 2 were adulterated with small amounts of other ingredients. 展开更多
关键词 Meat and meat products Multiple nucleotide polymorphism marker method High-throughput sequencing Animal-derived component identification
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Prediction of joint roughness coefficient via hybrid machine learning model combined with principal components analysis 被引量:1
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作者 Shijie Xie Hang Lin +2 位作者 Tianxing Ma Kang Peng Zhen Sun 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第4期2291-2306,共16页
Joint roughness coefficient(JRC)is the most commonly used parameter for quantifying surface roughness of rock discontinuities in practice.The system composed of multiple roughness statistical parameters to measure JRC... Joint roughness coefficient(JRC)is the most commonly used parameter for quantifying surface roughness of rock discontinuities in practice.The system composed of multiple roughness statistical parameters to measure JRC is a nonlinear system with a lot of overlapping information.In this paper,a dataset of eight roughness statistical parameters covering 112 digital joints is established.Then,the principal component analysis method is introduced to extract the significant information,which solves the information overlap problem of roughness characterization.Based on the two principal components of extracted features,the white shark optimizer algorithm was introduced to optimize the extreme gradient boosting model,and a new machine learning(ML)prediction model was established.The prediction accuracy of the new model and the other 17 models was measured using statistical metrics.The results show that the prediction result of the new model is more consistent with the real JRC value,with higher recognition accuracy and generalization ability. 展开更多
关键词 Rock discontinuities Joint roughness coefficient(JRC) Roughness characterization Principal components analysis(PCA) Machine learning
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