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Design of a Patrol and Security Robot with Semantic Mapping and Obstacle Avoidance System Using RGB-D Camera and LiDAR
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作者 Shu-Yin Chiang Shin-En Huang 《Computers, Materials & Continua》 2026年第4期1735-1753,共19页
This paper presents an intelligent patrol and security robot integrating 2D LiDAR and RGB-D vision sensors to achieve semantic simultaneous localization and mapping(SLAM),real-time object recognition,and dynamic obsta... This paper presents an intelligent patrol and security robot integrating 2D LiDAR and RGB-D vision sensors to achieve semantic simultaneous localization and mapping(SLAM),real-time object recognition,and dynamic obstacle avoidance.The system employs the YOLOv7 deep-learning framework for semantic detection and SLAM for localization and mapping,fusing geometric and visual data to build a high-fidelity 2D semantic map.This map enables the robot to identify and project object information for improved situational awareness.Experimental results show that object recognition reached 95.4%mAP@0.5.Semantic completeness increased from 68.7%(single view)to 94.1%(multi-view)with an average position error of 3.1 cm.During navigation,the robot achieved 98.0%reliability,avoided moving obstacles in 90.0%of encounters,and replanned paths in 0.42 s on average.The integration of LiDAR-based SLAMwith deep-learning–driven semantic perception establishes a robust foundation for intelligent,adaptive,and safe robotic navigation in dynamic environments. 展开更多
关键词 RGB-D semantic mapping object recognition obstacle avoidance security robot
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Electroacoustic tomography with dual-frequency array for real-time monitoring of electroporation
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作者 Luke Xu Yifei Xu Liangzhong Xiang 《Journal of Innovative Optical Health Sciences》 2026年第1期45-55,共11页
Electroacoustic Tomography(EAT)is an imaging technique that detects ultrasound waves induced by electrical pulses,offering a solution for real-time electroporation monitoring.This study presents EAT system using a dua... Electroacoustic Tomography(EAT)is an imaging technique that detects ultrasound waves induced by electrical pulses,offering a solution for real-time electroporation monitoring.This study presents EAT system using a dual-frequency ultrasound array.The broadband nature of electroacoustic signals requires ultrasound detector to cover both the high-frequency range(around 6MHz)signals generated by small targets and the low-frequency range(around 1MHz)signals generated by large targets.In our EAT system,we use the 6 MHz array to detect high-frequency signals from the electrodes,and the 1 MHz array for the electrical field.To test this,we conducted simulations using COMSOL Multiphysics^(®) and MATLAB's k-Wave toolbox,followed by experiments using a custom-built setup with a dual-frequency transducer and real-time data acquisition.The results demonstrated that the dual-frequency EAT system could accurately and simultaneously monitor the electroporation process,effectively showing both the treatment area and electrode placement with the application of 1 kV electric pulses with 100 ns duration.The axial resolution of the 6MHz array for EAT was 0.45 mm,significantly better than the 2mm resolution achieved with the 1MHz array.These findings validate the potential of dual-frequency EAT as a superior method for real-time electroporation monitoring. 展开更多
关键词 DUAL-FREQUENCY electroacoustic imaging real-time ELECTROPORATION
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DFCOA:Distributed Formation Control and Obstacle Avoidance for Multi-UGV Systems
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作者 Md.Faishal Rahaman Xueyuan Li +3 位作者 Muhammad Amjad Ibrahim Gasimove Md.Shariful Islam S.M.Abul Bashar 《Computer Modeling in Engineering & Sciences》 2026年第2期922-954,共33页
Researchers are increasingly focused on enabling groups of multiple unmanned vehicles to operate cohesively in complex,real-world environments,where coordinated formation control and obstacle avoidance are essential f... Researchers are increasingly focused on enabling groups of multiple unmanned vehicles to operate cohesively in complex,real-world environments,where coordinated formation control and obstacle avoidance are essential for executing sophisticated collective tasks.This paper presents a Distributed Formation Control and Obstacle Avoidance(DFCOA)framework for multi-unmanned ground vehicles(UGV).DFCOA integrates a virtual leader structure for global guidance,an improved A^(*)path planning algorithm with an advanced cost function for efficient path planning,and a repulsive-force-based improved vector field histogram star(VFH^(*))technique for collision avoidance.The virtual leader generates a reference trajectory while enabling distributed execution;the improved A^(*)algorithm reduces planning time and number of nodes to determine the shortest path from the starting position to the goal;and the improved VFH^(*)uses 2D LiDAR data with inter-agent repulsive force to simultaneously avoid collision with obstacles and maintain safe inter-vehicle distances.The formation stability of the proposed DFCOA reaches 95.8%and 94.6%in two scenarios,with root mean square(RMS)centroid errors of 0.9516 and 1.0008 m,respectively.Velocity tracking is precise(velocity centroid error RMS of 0.2699 and 0.1700 m/s),and linear velocities closely match the desired 0.3 m/s.Safety metrics showed average collision risks of 0.7773 and 0.5143,with minimum inter-vehicle distances of 0.4702 and 0.8763 m,confirming collision-free navigation of four UGVs.DFCOA outperforms conventional methods in formation stability,path efficiency,and scalability,proving its suitability for decentralized multi-UGV applications. 展开更多
关键词 Formation control obstacle avoidance virtual leader path planning multi UGV collaboration
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Semi-supervised Risk Assessment Research for Intelligent Vehicles Inspired by Collective Biological Risk-avoidance Behaviors
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作者 Hongyu Hu Zhonghua Xiong +2 位作者 Zhengyi Li Tianjun Sun Rui Ran 《Journal of Bionic Engineering》 2026年第1期225-238,共14页
To address the critical challenge of risk perception and assessment for autonomous vehicles in dynamic interactive envi-ronments,this study proposes a semi-supervised spatiotemporal interaction risk cognition network ... To address the critical challenge of risk perception and assessment for autonomous vehicles in dynamic interactive envi-ronments,this study proposes a semi-supervised spatiotemporal interaction risk cognition network with attention mecha-nism(SS-SIRCN),inspired by the behavioral adaptation patterns of biological groups under external threats.First,by thoroughly analyzing the dynamic interaction characteristics exhibited by typical biological collectives when exposed to risk,the study reveals the underlying patterns of trajectory changes influenced by external danger.Then,an attention-based spatiotemporal risk cognition network is designed to establish a mapping between driving behavior features and potential driving risks.Finally,a semi-supervised learning framework is employed to enable risk assessment for autono-mous vehicles using only a small amount of labeled data.Experimental results on real-world vehicle trajectory datasets demonstrate that the proposed method achieves a risk prediction accuracy of 90.76%,outperforming other baseline models in performance. 展开更多
关键词 Escape behaviour Predator avoidance Brain-like intelligent decision-making Attention mechanism Driving risk Automated driving
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Block-Wise Sliding Recursive Wavelet Transform and Its Application in Real-Time Vehicle-Induced Signal Separation
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作者 Jie Li Nan An Youliang Ding 《Structural Durability & Health Monitoring》 2026年第1期1-22,共22页
Vehicle-induced response separation is a crucial issue in structural health monitoring(SHM).This paper proposes a block-wise sliding recursive wavelet transform algorithm to meet the real-time processing requirements ... Vehicle-induced response separation is a crucial issue in structural health monitoring(SHM).This paper proposes a block-wise sliding recursive wavelet transform algorithm to meet the real-time processing requirements of monitoring data.To extend the separation target from a fixed dataset to a continuously updating data stream,a block-wise sliding framework is first developed.This framework is further optimized considering the characteristics of real-time data streams,and its advantage in computational efficiency is theoretically demonstrated.During the decomposition and reconstruction processes,information from neighboring data blocks is fully utilized to reduce algorithmic complexity.In addition,a delay-setting strategy is introduced for each processing window to mitigate boundary effects,thereby balancing accuracy and efficiency.Simulated signal experiments are conducted to determine the optimal delay configuration and to verify the algorithm’s superior performance,achieving a lower Root Mean Square Error(RMSE)and only 0.0249 times the average computational time compared with the original algorithm.Furthermore,strain signals from the Lieshi River Bridge are employed to validate the method.The proposed algorithm successfully separates the static trend from vehicle-induced responses in real time across different sampling frequencies,demonstrating its effectiveness and applicability in real-time bridge monitoring. 展开更多
关键词 Wavelet transform vehicle-induced signal separation real-time structure monitoring
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A Bio-inspired Bubble Artificial Muscles and TacTip Perception-driven Tri-legged Robot for Obstacle Avoidance
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作者 Chaoqun Xiang Zhengwei Zhong +3 位作者 Wenqiang Wu Xiaocong Chen Yisheng Guan Tao Zou 《Journal of Bionic Engineering》 2026年第1期175-191,共17页
Legged robots have considerable potential for traversing unstructured situations;nonetheless,their inflexible frameworks often constrain adaptability and obstacle negotiation.The study article presents a revolutionary... Legged robots have considerable potential for traversing unstructured situations;nonetheless,their inflexible frameworks often constrain adaptability and obstacle negotiation.The study article presents a revolutionary Soft Tri-Legged Robot(STLR)that improves movement and obstacle-avoidance skills by using a bio-inspired pneumatic artificial muscle(Bubble Artificial Muscles)and a bio-inspired tactile sensor(TacTip).The STLR is activated by BAMs,which are flexible,pneu-matic-driven actuators that provide fine control over forward,backward,and steering movements.Obstacle identification and avoidance are facilitated by the TacTip sensor,which delivers tactile input for traversing unstructured terrains.We delineate the mechanical features of the BAMs,assess the functionality of the robot's legs,and elaborate on the incorpora-tion of the tactile sensing system.Experimental results demonstrate that the STLR can effectively achieve multi-directional flexible movement and obstacle avoidance through a cross-modal perception-actuation mechanism.This study highlights the promise of soft robotics for search and rescue,medical aid,and autonomous exploration,while delineating difficulties and opportunities for future improvements in functionality and efficiency. 展开更多
关键词 Legged robot Bio-inspired bubble artificial muscles Bio-inspired TacTip sensor Foot tactile perception Obstacle avoidance
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Deep reinforcement learning-based adaptive collision avoidance method for UAV in joint operational airspace
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作者 Yan Shen Xuejun Zhang +1 位作者 Yan Li Weidong Zhang 《Defence Technology(防务技术)》 2026年第2期142-159,共18页
As joint operations have become a key trend in modern military development,unmanned aerial vehicles(UAVs)play an increasingly important role in enhancing the intelligence and responsiveness of combat systems.However,t... As joint operations have become a key trend in modern military development,unmanned aerial vehicles(UAVs)play an increasingly important role in enhancing the intelligence and responsiveness of combat systems.However,the heterogeneity of aircraft,partial observability,and dynamic uncertainty in operational airspace pose significant challenges to autonomous collision avoidance using traditional methods.To address these issues,this paper proposes an adaptive collision avoidance approach for UAVs based on deep reinforcement learning.First,a unified uncertainty model incorporating dynamic wind fields is constructed to capture the complexity of joint operational environments.Then,to effectively handle the heterogeneity between manned and unmanned aircraft and the limitations of dynamic observations,a sector-based partial observation mechanism is designed.A Dynamic Threat Prioritization Assessment algorithm is also proposed to evaluate potential collision threats from multiple dimensions,including time to closest approach,minimum separation distance,and aircraft type.Furthermore,a Hierarchical Prioritized Experience Replay(HPER)mechanism is introduced,which classifies experience samples into high,medium,and low priority levels to preferentially sample critical experiences,thereby improving learning efficiency and accelerating policy convergence.Simulation results show that the proposed HPER-D3QN algorithm outperforms existing methods in terms of learning speed,environmental adaptability,and robustness,significantly enhancing collision avoidance performance and convergence rate.Finally,transfer experiments on a high-fidelity battlefield airspace simulation platform validate the proposed method's deployment potential and practical applicability in complex,real-world joint operational scenarios. 展开更多
关键词 Unmanned aerial vehicle Collision avoidance Deep reinforcement learning Joint operational airspace Hierarchical prioritized experience replay
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Energy relief effect of real-time drilling to prevent rockburst in high-stress rock
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作者 Zhichao He Fengqiang Gong +2 位作者 Li Ren Weimin Yang Xuezhen Wu 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第2期1460-1475,共16页
To investigate the energy relief effect of real-time drilling in preventing rockburst in high-stress rock,a series of high-stress real-time drilling uniaxial compression tests were conducted on red sandstone specimens... To investigate the energy relief effect of real-time drilling in preventing rockburst in high-stress rock,a series of high-stress real-time drilling uniaxial compression tests were conducted on red sandstone specimens using the SG4500 drilling rig.Results showed that the mechanical behavior(i.e.peak strength and rockburst intensity)of the rock was weakened under high-stress real-time drilling and exhibited a downward trend as the drilling diameter increased.The real-time drilling energy dissipation index(ERD)was proposed to characterize the energy relief during high-stress real-time drilling.The ERD exhibited a linear increase with the real-time drilling diameter.Furthermore,the elastic strain energy of post-drilling rock showed a linear relationship with the square of stress across different stress levels,which also applied to the peak elastic strain energy and the square of peak stress.This findingreveals the intrinsic link between the weakening effect of peak elastic strain energy and peak strength due to high-stress real-time drilling,confirmingthe consistency between energy relief and pressure relief effects.By establishing relationships among rockburst proneness,peak elastic strain energy,and peak strength,it was demonstrated that high-stress real-time drilling reduces rockburst proneness through energy dissipation.Specifically,both peak elastic strain energy and rockburst proneness decreased with larger drill bit diameters,consistent with reductions in peak strength,rockburst intensity,and fractal dimensions of high-stress real-time drilled rock.These results validate the energy relief mechanism of real-time drilling in mitigating rockburst risks. 展开更多
关键词 Rock mechanics ROCKBURST real-time drilling Drilling energy relief Energy storage capacity Rockburst proneness
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Experimental study on real-time monitoring of surrounding rock 3D wave velocity structure and failure zone in deep tunnels
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作者 Hongyun Yang Chuandong Jiang +4 位作者 Yong Li Zhi Lin Xiang Wang Yifei Wu Wanlin Feng 《International Journal of Mining Science and Technology》 2026年第2期423-437,共15页
An innovative real-time monitoring method for surrounding rock damage based on microseismic time-lapse double-difference tomography is proposed for delayed dynamic damage identification and insufficient detection of a... An innovative real-time monitoring method for surrounding rock damage based on microseismic time-lapse double-difference tomography is proposed for delayed dynamic damage identification and insufficient detection of adverse geological conditions in deep-buried tunnel construction.The installation techniques for microseismic sensors were optimized by mounting sensors at bolt ends which significantly improves signal-to-noise ratio(SNR)and anti-interference capability compared to conventional borehole placement.Subsequently,a 3D wave velocity evolution model that incorporates construction-induced disturbances was established,enabling the first visualization of spatiotemporal variations in surrounding rock wave velocity.It finds significant wave velocity reduction near the tunnel face,with roof and floor damage zones extending 40–50 m;wave velocities approaching undisturbed levels at 15 m ahead of the working face and on the laterally undisturbed side;pronounced spatial asymmetry in wave velocity distribution—values on the left side exceed those on the right,with a clear stress concentration or transition zone located 10–15 m;and systematically lower velocities behind the face than in front,indicating asymmetric rock damage development.These results provide essential theoretical support and practical guidance for optimizing dynamic construction strategies,enabling real-time adjustment of support parameters,and establishing safety early warning systems in deep-buried tunnel engineering. 展开更多
关键词 Deep-buried tunnel Microseismic monitoring Wave velocity tomography Surrounding rock damage zone real-time monitoring
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A Hybrid Deep Learning Approach for Real-Time Cheating Behaviour Detection in Online Exams Using Video Captured Analysis
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作者 Dao Phuc Minh Huy Gia Nhu Nguyen Dac-Nhuong Le 《Computers, Materials & Continua》 2026年第3期1179-1198,共20页
Online examinations have become a dominant assessment mode,increasing concerns over academic integrity.To address the critical challenge of detecting cheating behaviours,this study proposes a hybrid deep learning appr... Online examinations have become a dominant assessment mode,increasing concerns over academic integrity.To address the critical challenge of detecting cheating behaviours,this study proposes a hybrid deep learning approach that combines visual detection and temporal behaviour classification.The methodology utilises object detection models—You Only Look Once(YOLOv12),Faster Region-based Convolutional Neural Network(RCNN),and Single Shot Detector(SSD)MobileNet—integrated with classification models such as Convolutional Neural Networks(CNN),Bidirectional Gated Recurrent Unit(Bi-GRU),and CNN-LSTM(Long Short-Term Memory).Two distinct datasets were used:the Online Exam Proctoring(EOP)dataset from Michigan State University and the School of Computer Science,Duy Tan Unievrsity(SCS-DTU)dataset collected in a controlled classroom setting.A diverse set of cheating behaviours,including book usage,unauthorised interaction,internet access,and mobile phone use,was categorised.Comprehensive experiments evaluated the models based on accuracy,precision,recall,training time,inference speed,and memory usage.We evaluate nine detector-classifier pairings under a unified budget and score them via a calibrated harmonic mean of detection and classification accuracies,enabling deployment-oriented selection under latency and memory constraints.Macro-Precision/Recall/F1 and Receiver Operating Characteristic-Area Under the Curve(ROC-AUC)are reported for the top configurations,revealing consistent advantages of object-centric pipelines for fine-grained cheating cues.The highest overall score is achieved by YOLOv12+CNN(97.15%accuracy),while SSD-MobileNet+CNN provides the best speed-efficiency trade-off for edge devices.This research provides valuable insights into selecting and deploying appropriate deep learning models for maintaining exam integrity under varying resource constraints. 展开更多
关键词 Online exam proctoring cheating behavior detection deep learning real-time monitoring object detection human behavior recognition
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Real-time decision support for bolter recovery safety:Long short-term memory network-driven aircraft sequencing
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作者 Wei Han Changjiu Li +4 位作者 Xichao Su Yong Zhang Fang Guo Tongtong Yu Xuan Li 《Defence Technology(防务技术)》 2026年第2期184-205,共22页
The highly dynamic nature,strong uncertainty,and coupled multiple safety constraints inherent in carrier aircraft recovery operations pose severe challenges for real-time decision-making.Addressing bolter scenarios,th... The highly dynamic nature,strong uncertainty,and coupled multiple safety constraints inherent in carrier aircraft recovery operations pose severe challenges for real-time decision-making.Addressing bolter scenarios,this study proposes an intelligent decision-making framework based on a deep long short-term memory Q-network.This framework transforms the real-time sequencing for bolter recovery problem into a partially observable Markov decision process.It employs a stacked long shortterm memory network to accurately capture the long-range temporal dependencies of bolter event chains and fuel consumption.Furthermore,it integrates a prioritized experience replay training mechanism to construct a safe and adaptive scheduling system capable of millisecond-level real-time decision-making.Experimental demonstrates that,within large-scale mass recovery scenarios,the framework achieves zero safety violations in static environments and maintains a fuel safety violation rate below 10%in dynamic scenarios,with single-step decision times at the millisecond level.The model exhibits strong generalization capability,effectively responding to unforeseen emergent situations—such as multiple bolters and fuel emergencies—without requiring retraining.This provides robust support for efficient carrier-based aircraft recovery operations. 展开更多
关键词 Carrier-based aircraft Recovery scheduling Deep reinforcement learning Long short-term memory networks Dynamic real-time decision-making
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Extended dynamic system modulation for real-time obstacle avoidance 被引量:5
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作者 Zhide ZHANG Zhengjie WANG Jin YU 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2022年第12期212-225,共14页
In this paper,a novel real-time obstacle avoidance method based on Dynamic System(DS),is proposed.The proposed method ensures the impenetrability of multiple convex obstacles by online modulating the original velocity... In this paper,a novel real-time obstacle avoidance method based on Dynamic System(DS),is proposed.The proposed method ensures the impenetrability of multiple convex obstacles by online modulating the original velocity field of the DS.It can be applied to perform obstacle avoidance in the state space of the DS with both autonomous and non-autonomous DS-based controllers.While realizing the obstacle avoidance,the equilibrium points of the original DS can be saved.The modulation matrix form is extended based on the earlier dynamic system modulation methods of the literature.The asymmetric modulation provided by this method allows the modulated DS to satisfy the dynamic constraints of a class of DSs.In addition,the proposed method has the inherent ability of multiple-obstacle avoidance and the direction selectivity of avoidance maneuver.Moreover,to solve the simultaneous guidance and obstacle avoidance problem,a guidance law for Unmanned Aerial Vehicles(UAVs)based on the proposed method,is designed.Finally,a numerical simulation is performed to analyze the performance of the proposed method and the obstacle avoidance guidance law. 展开更多
关键词 Collision avoidance Dynamic system GUIDANCE MODULATION Unmanned Aerial Vehicles(UAVs)
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Real-Time Fuzzy Obstacle Avoidance Using Directional Visual Perception
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作者 黄国权 RadA.B. WongY.K. 《Journal of Southwest Jiaotong University(English Edition)》 2004年第2期107-115,共9页
This paper presents a novel vision-based obstacle avoidance approach for the Autonomous Mobile Robot (AMR) with a Pan-Tilt-Zoom (PTZ) camera as its only sensing modality. The approach combines the morphological closin... This paper presents a novel vision-based obstacle avoidance approach for the Autonomous Mobile Robot (AMR) with a Pan-Tilt-Zoom (PTZ) camera as its only sensing modality. The approach combines the morphological closing operation based on Sobel Edge Detection Operation and the (μ-kσ) thresholding technique to detect obstacles to soften the various lighting and ground floor effects. Both the morphology method and thresholding technique are computationally simple. The processing speed of the algorithm is fast enough to avoid some active obstacles. In addition, this approach takes into account the history obstacle effects on the current state. Fuzzy logic is used to control the behaviors of AMR as it navigates in the environment. All behaviors run concurrently and generate motor response solely based on vision perception. A priority based on subsumption coordinator selects the most appropriate response to direct the AMR away from obstacles. Validation of the proposed approach is done on a Pioneer 1 mobile robot. 展开更多
关键词 Fuzzy system Obstacle avoidance Edge detection Autonomous mobile robot
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Real-Time Arm Animation with Natural Collision Avoidance
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作者 倪慧 孙济洲 刘艳 《Transactions of Tianjin University》 EI CAS 2007年第5期365-369,共5页
A novel approach to realistic collision-free animation of the upper limb was proposed.According to the obstacle-avoidance strategy of human hand,the movement trajectory was computed by manipulability model and minimum... A novel approach to realistic collision-free animation of the upper limb was proposed.According to the obstacle-avoidance strategy of human hand,the movement trajectory was computed by manipulability model and minimum-jerk based interpolation.In each key frame,an improved in~verse kinematics method was adopted to obtain a believable posture of the upper limb.By comparing with the real movement data obtained from the motion capture device,the resultant animation was testified to be natural.This method can be performed in interactive time,and therefore is applicable in animation edit and control of virtual humans. 展开更多
关键词 ANIMATION collision avoidance manipulability model INTERPOLATION
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Application of A* Algorithm for Real-time Path Re-planning of an Unmanned Surface Vehicle Avoiding Underwater Obstacles 被引量:9
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作者 Thanapong Phanthong Toshihiro Maki +2 位作者 Tamaki Ura Takashi Sakamaki Pattara Aiyarak 《Journal of Marine Science and Application》 2014年第1期105-116,共12页
This paper describes path re-planning techniques and underwater obstacle avoidance for unmanned surface vehicle(USV) based on multi-beam forward looking sonar(FLS). Near-optimal paths in static and dynamic environment... This paper describes path re-planning techniques and underwater obstacle avoidance for unmanned surface vehicle(USV) based on multi-beam forward looking sonar(FLS). Near-optimal paths in static and dynamic environments with underwater obstacles are computed using a numerical solution procedure based on an A* algorithm. The USV is modeled with a circular shape in 2 degrees of freedom(surge and yaw). In this paper, two-dimensional(2-D) underwater obstacle avoidance and the robust real-time path re-planning technique for actual USV using multi-beam FLS are developed. Our real-time path re-planning algorithm has been tested to regenerate the optimal path for several updated frames in the field of view of the sonar with a proper update frequency of the FLS. The performance of the proposed method was verified through simulations, and sea experiments. For simulations, the USV model can avoid both a single stationary obstacle, multiple stationary obstacles and moving obstacles with the near-optimal trajectory that are performed both in the vehicle and the world reference frame. For sea experiments, the proposed method for an underwater obstacle avoidance system is implemented with a USV test platform. The actual USV is automatically controlled and succeeded in its real-time avoidance against the stationary undersea obstacle in the field of view of the FLS together with the Global Positioning System(GPS) of the USV. 展开更多
关键词 UNDERWATER OBSTACLE avoidance real-time pathre-planning A* ALGORITHM SONAR image unmanned surface vehicle
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奶牛乳房炎病原体三重Real-time PCR检测方法的建立及应用
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作者 郭思宇 高雅欣 +5 位作者 纪佳豪 李梓豪 刘文扬 徐博 王三毛 李睿文 《动物医学进展》 北大核心 2025年第12期39-44,共6页
为了建立同时检测奶牛临床型乳房炎中肺炎克雷伯菌(Kp)、产色葡萄球菌(Sc)和牛支原体(Mb),基于Kp ZKIR基因、Sc sodA基因和Mb opp D/F基因设计特异性引物,建立三重实时定量荧光PCR方法(real-time PCR)。试验采用在单一real-time PCR检... 为了建立同时检测奶牛临床型乳房炎中肺炎克雷伯菌(Kp)、产色葡萄球菌(Sc)和牛支原体(Mb),基于Kp ZKIR基因、Sc sodA基因和Mb opp D/F基因设计特异性引物,建立三重实时定量荧光PCR方法(real-time PCR)。试验采用在单一real-time PCR检测方法的基础上对三重real-time PCR检测方法进行优化,并确定退火条件为60℃,肺炎克雷伯菌、产色葡萄球菌以及牛支原体上、下游引物浓度为20μmol/L、荧光探针浓度为10μmol/L。结果表明,该方法对标准品pUC57-ZKIR-Kp、pUC57-sodA-Sc、pUC57-opp D/F-Mb最低检测限分别为1.55×10^(2) copies/μL、1.44×10^(2) copies/μL、1.34×10^(2) copies/μL,灵敏度高;仅对Kp、Sc、Mb这3种病原产生荧光曲线,对其他病原无交叉反应,特异性强;其中组内、组间变异系数均小于2%,重复性良好。利用建立的多重real-time PCR对233份临床样品进行检测,Kp、Sc、Mb检出率分别为73.09%、21.97%、6.72%,与单一real-time PCR方法检测结果一致。说明建立的多重real-time PCR在实际应用中具有灵敏度高、特异性强、重复性良好、检测速度快等优点,可为奶牛临床型乳房炎病原的快速检测、临床诊断和流行病学调查提供有效检测手段。 展开更多
关键词 临床型乳房炎 三重real-time PCR 肺炎克雷伯菌 产色葡萄球菌 牛支原体
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Real-time monitoring of disc cutter wear in tunnel boring machines:A sound and vibration sensor-based approach with machine learning technique 被引量:1
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作者 Mohammad Amir Akhlaghi Raheb Bagherpour Seyed Hadi Hoseinie 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第3期1700-1722,共23页
Large portions of the tunnel boring machine(TBM)construction cost are attributed to disc cutter consumption,and assessing the disc cutter's wear level can help determine the optimal time to replace the disc cutter... Large portions of the tunnel boring machine(TBM)construction cost are attributed to disc cutter consumption,and assessing the disc cutter's wear level can help determine the optimal time to replace the disc cutter.Therefore,the need to monitor disc cutter wear in real-time has emerged as a technical challenge for TBMs.In this study,real-time disc cutter wear monitoring is developed based on sound and vibration sensors.For this purpose,the microphone and accelerometer were used to record the sound and vibration signals of cutting three different types of rocks with varying abrasions on a laboratory scale.The relationship between disc cutter wear and the sound and vibration signal was determined by comparing the measurements of disc cutter wear with the signal plots for each sample.The features extracted from the signals showed that the sound and vibration signals are impacted by the progression of disc wear during the rock-cutting process.The signal features obtained from the rock-cutting operation were utilized to verify the machine learning techniques.The results showed that the multilayer perceptron(MLP),random subspace-based decision tree(RS-DT),DT,and random forest(RF)methods could predict the wear level of the disc cutter with an accuracy of 0.89,0.951,0.951,and 0.927,respectively.Based on the accuracy of the models and the confusion matrix,it was found that the RS-DT model has the best estimate for predicting the level of disc wear.This research has developed a method that can potentially determine when to replace a tool and assess disc wear in real-time. 展开更多
关键词 TBM disc cutter WEAR SOUND VIBRATION Machine learning real-time wear estimation
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Intelligent sequential multi-impulse collision avoidance method for non-cooperative spacecraft based on an improved search tree algorithm 被引量:1
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作者 Xuyang CAO Xin NING +4 位作者 Zheng WANG Suyi LIU Fei CHENG Wenlong LI Xiaobin LIAN 《Chinese Journal of Aeronautics》 2025年第4期378-393,共16页
The problem of collision avoidance for non-cooperative targets has received significant attention from researchers in recent years.Non-cooperative targets exhibit uncertain states and unpredictable behaviors,making co... The problem of collision avoidance for non-cooperative targets has received significant attention from researchers in recent years.Non-cooperative targets exhibit uncertain states and unpredictable behaviors,making collision avoidance significantly more challenging than that for space debris.Much existing research focuses on the continuous thrust model,whereas the impulsive maneuver model is more appropriate for long-duration and long-distance avoidance missions.Additionally,it is important to minimize the impact on the original mission while avoiding noncooperative targets.On the other hand,the existing avoidance algorithms are computationally complex and time-consuming especially with the limited computing capability of the on-board computer,posing challenges for practical engineering applications.To conquer these difficulties,this paper makes the following key contributions:(A)a turn-based(sequential decision-making)limited-area impulsive collision avoidance model considering the time delay of precision orbit determination is established for the first time;(B)a novel Selection Probability Learning Adaptive Search-depth Search Tree(SPL-ASST)algorithm is proposed for non-cooperative target avoidance,which improves the decision-making efficiency by introducing an adaptive-search-depth mechanism and a neural network into the traditional Monte Carlo Tree Search(MCTS).Numerical simulations confirm the effectiveness and efficiency of the proposed method. 展开更多
关键词 Non-cooperative target Collision avoidance Limited motion area Impulsive maneuver model Search tree algorithm Neural networks
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A literature review and outlook of advertising avoidance:An integrated theoretical framework based on the SOMR model
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作者 Shengliang Zhang Jianhui Jin Xiaodong Li 《中国科学技术大学学报》 北大核心 2025年第1期2-16,1,I0001,共17页
Advertising avoidance is resistance to advertising intrusion.This issue has been the subject of much academic research in recent years.To guide scholars to better carry out relevant research and promote enterprises to... Advertising avoidance is resistance to advertising intrusion.This issue has been the subject of much academic research in recent years.To guide scholars to better carry out relevant research and promote enterprises to better implement advertising activities,this study intends to summarize the relevant research on advertising avoidance in recent years.The specific method is to use the core literature meta-analysis method to identify,filter,and screen relevant literature published in core journals from 1997 to 2020 with the keywords advertising avoidance and advertising resistance.We review the collected articles from the following perspectives:the definition and classification,external stimulating factors,internal perception factors,and moderating factors of advertising avoidance.On this basis,the SOMR model of advertising avoidance is constructed according to the SOR model.Finally,some prospects for future related research are presented. 展开更多
关键词 ADVERTISING advertising avoidance SOMR model perceived value perceived infringement
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Fluid-based moderate collision avoidance for UAV formation in 3-D low-altitude environments 被引量:1
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作者 Menghua ZHANG Honglun WANG +5 位作者 Zhiyu LI Yanxiang WANG Xianglun ZHANG Qiang TANG Shichao MA Jianfa WU 《Chinese Journal of Aeronautics》 2025年第6期533-551,共19页
Aiming to address the Unmanned Aerial Vehicle(UAV) formation collision avoidance problem in Three-Dimensional(3-D) low-altitude environments where dense various obstacles exist, a fluid-based path planning framework n... Aiming to address the Unmanned Aerial Vehicle(UAV) formation collision avoidance problem in Three-Dimensional(3-D) low-altitude environments where dense various obstacles exist, a fluid-based path planning framework named the Formation Interfered Fluid Dynamical System(FIFDS) with Moderate Evasive Maneuver Strategy(MEMS) is proposed in this study.First, the UAV formation collision avoidance problem including quantifiable performance indexes is formulated. Second, inspired by the phenomenon of fluids continuously flowing while bypassing objects, the FIFDS for multiple UAVs is presented, which contains a Parallel Streamline Tracking(PST) method for formation keeping and the traditional IFDS for collision avoidance. Third, to rationally balance flight safety and collision avoidance cost, MEMS is proposed to generate moderate evasive maneuvers that match up with collision risks. Comprehensively containing the time and distance safety information, the 3-D dynamic collision regions are modeled for collision prediction. Then, the moderate evasive maneuver principle is refined, which provides criterions of the maneuver amplitude and direction. On this basis, an analytical parameter mapping mechanism is designed to online optimize IFDS parameters. Finally, the performance of the proposed method is validated by comparative simulation results and real flight experiments using fixed-wing UAVs. 展开更多
关键词 Unmanned aerial vehicle Formation collision avoidance:3-D low-altitude environments Interfered fluid dynamical system 3-D dynamic collision region
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