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Real-time seepage and instability of fractured granite subjected to hydro-shearing under different critical slip states
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作者 Peng Zhao Zijun Feng +3 位作者 Hanmo Nan Peihua Jin Chunsheng Deng Yubin Li 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第4期2396-2415,共20页
In this study,a high-confining pressure and real-time large-displacement shearing-flow setup was developed.The test setup can be used to analyze the injection pressure conditions that increase the hydro-shearing perme... In this study,a high-confining pressure and real-time large-displacement shearing-flow setup was developed.The test setup can be used to analyze the injection pressure conditions that increase the hydro-shearing permeability and injection-induced seismicity during hot dry rock geothermal extraction.For optimizing injection strategies and improving engineering safety,real-time permeability,deformation,and energy release characteristics of fractured granite samples driven by injected water pressure under different critical sliding conditions were evaluated.The results indicated that:(1)A low injection water pressure induced intermittent small-deformation stick-slip behavior in fractures,and a high injection pressure primarily caused continuous high-speed large-deformation sliding in fractures.The optimal injection water pressure range was defined for enhancing hydraulic shear permeability and preventing large injection-induced earthquakes.(2)Under the same experimental conditions,fracture sliding was deemed as the major factor that enhanced the hydraulic shear-permeability enhancement and the maximum permeability increased by 36.54 and 41.59 times,respectively,in above two slip modes.(3)Based on the real-time transient evolution of water pressure during fracture sliding,the variation coefficients of slip rate,permeability,and water pressure were fitted,and the results were different from those measured under quasi-static conditions.(4)The maximum and minimum shear strength criteria for injection-induced fracture sliding were also determined(μ=0.6665 andμ=0.1645,respectively,μis friction coefficient).Using the 3D(three-dimensional)fracture surface scanning technology,the weakening effect of injection pressure on fracture surface damage characteristics was determined,which provided evidence for the geological markers of fault sliding mode and sliding nature transitions under the fluid influence. 展开更多
关键词 Hydro-shearing Reservoir modification Injection-induced seismicity real-time shear-flowing Frictional noise
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Long-release-interval-first real-time scheduling algorithm and its schedulability test
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作者 沈卓炜 汪芸 《Journal of Southeast University(English Edition)》 EI CAS 2006年第4期484-489,共6页
To fulfill the requirements for hybrid real-time system scheduling, a long-release-interval-first (LRIF) real-time scheduling algorithm is proposed. The algorithm adopts both the fixed priority and the dynamic prior... To fulfill the requirements for hybrid real-time system scheduling, a long-release-interval-first (LRIF) real-time scheduling algorithm is proposed. The algorithm adopts both the fixed priority and the dynamic priority to assign priorities for tasks. By assigning higher priorities to the aperiodic soft real-time jobs with longer release intervals, it guarantees the executions for periodic hard real-time tasks and further probabilistically guarantees the executions for aperiodic soft real-time tasks. The schedulability test approach for the LRIF algorithm is presented. The implementation issues of the LRIF algorithm are also discussed. Simulation result shows that LRIF obtains better schedulable performance than the maximum urgency first (MUF) algorithm, the earliest deadline first (EDF) algorithm and EDF for hybrid tasks. LRIF has great capability to schedule both periodic hard real-time and aperiodic soft real-time tasks. 展开更多
关键词 real-time scheduling schedulability test earliest deadline first maximum urgency first long release interval first
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PIDINet-MC:Real-Time Multi-Class Edge Detection with PiDiNet
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作者 Mingming Huang Yunfan Ye Zhiping Cai 《Computers, Materials & Continua》 2026年第2期1983-1999,共17页
As a fundamental component in computer vision,edges can be categorized into four types based on discontinuities in reflectance,illumination,surface normal,or depth.While deep CNNs have significantly advanced generic e... As a fundamental component in computer vision,edges can be categorized into four types based on discontinuities in reflectance,illumination,surface normal,or depth.While deep CNNs have significantly advanced generic edge detection,real-time multi-class semantic edge detection under resource constraints remains challenging.To address this,we propose a lightweight framework based on PiDiNet that enables fine-grained semantic edge detection.Our model simultaneously predicts background and four edge categories from full-resolution inputs,balancing accuracy and efficiency.Key contributions include:a multi-channel output structure expanding binary edge prediction to five classes,supported by a deep supervision mechanism;a dynamic class-balancing strategy combining adaptive weighting with physical priors to handle extreme class imbalance;and maintained architectural efficiency enabling real-time inference.Extensive evaluations on BSDS-RIND show our approach achieves accuracy competitive with state-of-the-art methods while operating in real time. 展开更多
关键词 Multi-class edge detection real-time LIGHTWEIGHT deep supervision
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Dynamic psychological vulnerability and adaptation in rheumatoid arthritis:Trajectories,predictors,and interventions
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作者 Xue-Meng Chen Xian Cheng Wei Wu 《World Journal of Psychiatry》 2026年第1期32-46,共15页
Rheumatoid arthritis(RA)patients face significant psychological challenges alongside physical symptoms,necessitating a comprehensive understanding of how psychological vulnerability and adaptation patterns evolve thro... Rheumatoid arthritis(RA)patients face significant psychological challenges alongside physical symptoms,necessitating a comprehensive understanding of how psychological vulnerability and adaptation patterns evolve throughout the disease course.This review examined 95 studies(2000-2025)from PubMed,Web of Science,and CNKI databases including longitudinal cohorts,randomized controlled trials,and mixed-methods research,to characterize the complex interplay between biological,psychological,and social factors affecting RA patients’mental health.Findings revealed three distinct vulnerability trajectories(45%persistently low,30%fluctuating improvement,25%persistently high)and four adaptation stages,with critical intervention periods occurring 3-6 months postdiagnosis and during disease flares.Multiple factors significantly influence psychological outcomes,including gender(females showing 1.8-fold increased risk),age(younger patients experiencing 42%higher vulnerability),pain intensity,inflammatory markers,and neuroendocrine dysregulation(48%showing cortisol rhythm disruption).Early psychological intervention(within 3 months of diagnosis)demonstrated robust benefits,reducing depression incidence by 42%with effects persisting 24-36 months,while different modalities showed complementary advantages:Cognitive behavioral therapy for depression(Cohen’s d=0.68),mindfulness for pain acceptance(38%improvement),and peer support for meaning reconstruction(25.6%increase).These findings underscore the importance of integrating routine psychological assessment into standard RA care,developing stage-appropriate interventions,and advancing research toward personalized biopsychosocial approaches that address the dynamic psychological dimensions of the disease. 展开更多
关键词 Rheumatoid arthritis Psychological vulnerability Disease adaptation ability Dynamic changes Mental health
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Block-Wise Sliding Recursive Wavelet Transform and Its Application in Real-Time Vehicle-Induced Signal Separation
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作者 Jie Li Nan An Youliang Ding 《Structural Durability & Health Monitoring》 2026年第1期1-22,共22页
Vehicle-induced response separation is a crucial issue in structural health monitoring(SHM).This paper proposes a block-wise sliding recursive wavelet transform algorithm to meet the real-time processing requirements ... Vehicle-induced response separation is a crucial issue in structural health monitoring(SHM).This paper proposes a block-wise sliding recursive wavelet transform algorithm to meet the real-time processing requirements of monitoring data.To extend the separation target from a fixed dataset to a continuously updating data stream,a block-wise sliding framework is first developed.This framework is further optimized considering the characteristics of real-time data streams,and its advantage in computational efficiency is theoretically demonstrated.During the decomposition and reconstruction processes,information from neighboring data blocks is fully utilized to reduce algorithmic complexity.In addition,a delay-setting strategy is introduced for each processing window to mitigate boundary effects,thereby balancing accuracy and efficiency.Simulated signal experiments are conducted to determine the optimal delay configuration and to verify the algorithm’s superior performance,achieving a lower Root Mean Square Error(RMSE)and only 0.0249 times the average computational time compared with the original algorithm.Furthermore,strain signals from the Lieshi River Bridge are employed to validate the method.The proposed algorithm successfully separates the static trend from vehicle-induced responses in real time across different sampling frequencies,demonstrating its effectiveness and applicability in real-time bridge monitoring. 展开更多
关键词 Wavelet transform vehicle-induced signal separation real-time structure monitoring
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The Impact of Early Postoperative Rehabilitation Training on Balance Ability and Quality of Life in Elderly Patients with Hip Fracture
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作者 Kaiyu Yang Xiaofang Peng Li Xu 《Journal of Clinical and Nursing Research》 2026年第1期394-399,共6页
Objective:To analyze the improvement effect of early postoperative rehabilitation training on balance ability and quality of life in elderly patients with hip fracture.Methods:A total of 50 elderly patients with hip f... Objective:To analyze the improvement effect of early postoperative rehabilitation training on balance ability and quality of life in elderly patients with hip fracture.Methods:A total of 50 elderly patients with hip fracture admitted to our hospital from January 2023 to January 2024 were selected and divided into the observation group(25 cases)and the control group(25 cases)by random number table method.The control group received routine nursing,while the observation group received early rehabilitation training on the basis of routine nursing.The balance ability(Berg Balance Scale,BBS)and quality of life(SF-36)of the two groups were compared.Results:The BBS scores of the observation group at all postoperative time points were significantly higher than those of the control group(p<0.05),and the quality-of-life scores of the observation group were also significantly higher than those of the control group(p<0.05).Conclusion:Early postoperative rehabilitation training for elderly patients with hip fracture can improve their balance ability,enhance their quality of life,and reduce the incidence of postoperative complications,which is worthy of clinical promotion. 展开更多
关键词 Elderly hip fracture Early postoperative rehabilitation training Balance ability Quality of life COMPLICATIONS
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Energy relief effect of real-time drilling to prevent rockburst in high-stress rock
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作者 Zhichao He Fengqiang Gong +2 位作者 Li Ren Weimin Yang Xuezhen Wu 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第2期1460-1475,共16页
To investigate the energy relief effect of real-time drilling in preventing rockburst in high-stress rock,a series of high-stress real-time drilling uniaxial compression tests were conducted on red sandstone specimens... To investigate the energy relief effect of real-time drilling in preventing rockburst in high-stress rock,a series of high-stress real-time drilling uniaxial compression tests were conducted on red sandstone specimens using the SG4500 drilling rig.Results showed that the mechanical behavior(i.e.peak strength and rockburst intensity)of the rock was weakened under high-stress real-time drilling and exhibited a downward trend as the drilling diameter increased.The real-time drilling energy dissipation index(ERD)was proposed to characterize the energy relief during high-stress real-time drilling.The ERD exhibited a linear increase with the real-time drilling diameter.Furthermore,the elastic strain energy of post-drilling rock showed a linear relationship with the square of stress across different stress levels,which also applied to the peak elastic strain energy and the square of peak stress.This findingreveals the intrinsic link between the weakening effect of peak elastic strain energy and peak strength due to high-stress real-time drilling,confirmingthe consistency between energy relief and pressure relief effects.By establishing relationships among rockburst proneness,peak elastic strain energy,and peak strength,it was demonstrated that high-stress real-time drilling reduces rockburst proneness through energy dissipation.Specifically,both peak elastic strain energy and rockburst proneness decreased with larger drill bit diameters,consistent with reductions in peak strength,rockburst intensity,and fractal dimensions of high-stress real-time drilled rock.These results validate the energy relief mechanism of real-time drilling in mitigating rockburst risks. 展开更多
关键词 Rock mechanics ROCKBURST real-time drilling Drilling energy relief Energy storage capacity Rockburst proneness
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Experimental study on real-time monitoring of surrounding rock 3D wave velocity structure and failure zone in deep tunnels
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作者 Hongyun Yang Chuandong Jiang +4 位作者 Yong Li Zhi Lin Xiang Wang Yifei Wu Wanlin Feng 《International Journal of Mining Science and Technology》 2026年第2期423-437,共15页
An innovative real-time monitoring method for surrounding rock damage based on microseismic time-lapse double-difference tomography is proposed for delayed dynamic damage identification and insufficient detection of a... An innovative real-time monitoring method for surrounding rock damage based on microseismic time-lapse double-difference tomography is proposed for delayed dynamic damage identification and insufficient detection of adverse geological conditions in deep-buried tunnel construction.The installation techniques for microseismic sensors were optimized by mounting sensors at bolt ends which significantly improves signal-to-noise ratio(SNR)and anti-interference capability compared to conventional borehole placement.Subsequently,a 3D wave velocity evolution model that incorporates construction-induced disturbances was established,enabling the first visualization of spatiotemporal variations in surrounding rock wave velocity.It finds significant wave velocity reduction near the tunnel face,with roof and floor damage zones extending 40–50 m;wave velocities approaching undisturbed levels at 15 m ahead of the working face and on the laterally undisturbed side;pronounced spatial asymmetry in wave velocity distribution—values on the left side exceed those on the right,with a clear stress concentration or transition zone located 10–15 m;and systematically lower velocities behind the face than in front,indicating asymmetric rock damage development.These results provide essential theoretical support and practical guidance for optimizing dynamic construction strategies,enabling real-time adjustment of support parameters,and establishing safety early warning systems in deep-buried tunnel engineering. 展开更多
关键词 Deep-buried tunnel Microseismic monitoring Wave velocity tomography Surrounding rock damage zone real-time monitoring
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A Hybrid Deep Learning Approach for Real-Time Cheating Behaviour Detection in Online Exams Using Video Captured Analysis
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作者 Dao Phuc Minh Huy Gia Nhu Nguyen Dac-Nhuong Le 《Computers, Materials & Continua》 2026年第3期1179-1198,共20页
Online examinations have become a dominant assessment mode,increasing concerns over academic integrity.To address the critical challenge of detecting cheating behaviours,this study proposes a hybrid deep learning appr... Online examinations have become a dominant assessment mode,increasing concerns over academic integrity.To address the critical challenge of detecting cheating behaviours,this study proposes a hybrid deep learning approach that combines visual detection and temporal behaviour classification.The methodology utilises object detection models—You Only Look Once(YOLOv12),Faster Region-based Convolutional Neural Network(RCNN),and Single Shot Detector(SSD)MobileNet—integrated with classification models such as Convolutional Neural Networks(CNN),Bidirectional Gated Recurrent Unit(Bi-GRU),and CNN-LSTM(Long Short-Term Memory).Two distinct datasets were used:the Online Exam Proctoring(EOP)dataset from Michigan State University and the School of Computer Science,Duy Tan Unievrsity(SCS-DTU)dataset collected in a controlled classroom setting.A diverse set of cheating behaviours,including book usage,unauthorised interaction,internet access,and mobile phone use,was categorised.Comprehensive experiments evaluated the models based on accuracy,precision,recall,training time,inference speed,and memory usage.We evaluate nine detector-classifier pairings under a unified budget and score them via a calibrated harmonic mean of detection and classification accuracies,enabling deployment-oriented selection under latency and memory constraints.Macro-Precision/Recall/F1 and Receiver Operating Characteristic-Area Under the Curve(ROC-AUC)are reported for the top configurations,revealing consistent advantages of object-centric pipelines for fine-grained cheating cues.The highest overall score is achieved by YOLOv12+CNN(97.15%accuracy),while SSD-MobileNet+CNN provides the best speed-efficiency trade-off for edge devices.This research provides valuable insights into selecting and deploying appropriate deep learning models for maintaining exam integrity under varying resource constraints. 展开更多
关键词 Online exam proctoring cheating behavior detection deep learning real-time monitoring object detection human behavior recognition
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Real-time decision support for bolter recovery safety:Long short-term memory network-driven aircraft sequencing
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作者 Wei Han Changjiu Li +4 位作者 Xichao Su Yong Zhang Fang Guo Tongtong Yu Xuan Li 《Defence Technology(防务技术)》 2026年第2期184-205,共22页
The highly dynamic nature,strong uncertainty,and coupled multiple safety constraints inherent in carrier aircraft recovery operations pose severe challenges for real-time decision-making.Addressing bolter scenarios,th... The highly dynamic nature,strong uncertainty,and coupled multiple safety constraints inherent in carrier aircraft recovery operations pose severe challenges for real-time decision-making.Addressing bolter scenarios,this study proposes an intelligent decision-making framework based on a deep long short-term memory Q-network.This framework transforms the real-time sequencing for bolter recovery problem into a partially observable Markov decision process.It employs a stacked long shortterm memory network to accurately capture the long-range temporal dependencies of bolter event chains and fuel consumption.Furthermore,it integrates a prioritized experience replay training mechanism to construct a safe and adaptive scheduling system capable of millisecond-level real-time decision-making.Experimental demonstrates that,within large-scale mass recovery scenarios,the framework achieves zero safety violations in static environments and maintains a fuel safety violation rate below 10%in dynamic scenarios,with single-step decision times at the millisecond level.The model exhibits strong generalization capability,effectively responding to unforeseen emergent situations—such as multiple bolters and fuel emergencies—without requiring retraining.This provides robust support for efficient carrier-based aircraft recovery operations. 展开更多
关键词 Carrier-based aircraft Recovery scheduling Deep reinforcement learning Long short-term memory networks Dynamic real-time decision-making
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Real-time and reliability analysis of time-triggered CAN-bus 被引量:17
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作者 Xia Jiqiang Zhang Chuansen +1 位作者 Bai Ronggang Xue Liqiang 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2013年第1期171-178,共8页
Real-time performance and reliability are two most important issues in applications of time-triggered controller area network (CAN) bus systems at present. A scheduling matrix of time-triggered CAN-bus system is est... Real-time performance and reliability are two most important issues in applications of time-triggered controller area network (CAN) bus systems at present. A scheduling matrix of time-triggered CAN-bus system is established using average-loading algorithm. Periodic messages are guaranteed to transmit without delay by distributing independent transmission windows within the system matrix. Considering the traditional CAN-bus transmission mechanism and the time-triggered feature, an algorithm is improved to calculate the worst-case delay of event-triggered messages in time-triggered CAN-bus systems. The failure probability is calculated for event-triggered messages whose worst-case delay exceeds their deadlines. Different levels of redundant structures of CAN-bus circuits are analyzed and the maintenance management is proposed to improve the system reliability. Finally, the reliabilities of different structures are calculated and the influences of maintenance on the system reliability are analyzed. 展开更多
关键词 CAN-BUS Maintenance system real-time performance RELIability TIME-TRIGGERED
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Real-time reliability evaluation based on damaged measurement degradation data 被引量:16
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作者 王小林 蒋平 +1 位作者 郭波 程志君 《Journal of Central South University》 SCIE EI CAS 2012年第11期3162-3169,共8页
A method was proposed to evaluate the real-time reliability for a single product based on damaged measurement degradation data.Most researches on degradation analysis often assumed that the measurement process did not... A method was proposed to evaluate the real-time reliability for a single product based on damaged measurement degradation data.Most researches on degradation analysis often assumed that the measurement process did not have any impact on the product's performance.However,in some cases,the measurement process may exert extra stress on products being measured.To obtain trustful results in such a situation,a new degradation model was derived.Then,by fusing the prior information of product and its own on-line degradation data,the real-time reliability was evaluated on the basis of Bayesian formula.To make the proposed method more practical,a procedure based on expectation maximization (EM) algorithm was presented to estimate the unknown parameters.Finally,the performance of the proposed method was illustrated by a simulation study.The results show that ignoring the influence of the damaged measurement process can lead to biased evaluation results,if the damaged measurement process is involved. 展开更多
关键词 degradation analysis damaged measurement real-time reliability expectation maximization algorithm
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Stability and remote real-time monitoring of the slope slide body in the Luoshan mining area 被引量:7
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作者 Yang Xiaojie Hou Dinggui +2 位作者 Tao Zhigang Peng Yanyan Shi Haiyang 《International Journal of Mining Science and Technology》 SCIE EI CSCD 2015年第5期761-765,共5页
Landslides have occurred frequently in the Luoshan mining area because of disordered mining.This paper discusses the landforms and physiognomy,hydro-meteorology,formation lithology,and geologic structure of the Luosha... Landslides have occurred frequently in the Luoshan mining area because of disordered mining.This paper discusses the landforms and physiognomy,hydro-meteorology,formation lithology,and geologic structure of the Luoshan mining area.It also describes the factors influencing the slope stability of landslide No.Ⅲ,determines the general parameters and typical section plane,analyzes the stress-strain state of the No.Ⅲ slope,and calculates its safety factors with FLAC3 D under saturated and natural conditions.Based on a stability analysis,a remote real-time monitoring system was applied to the No.Ⅲ slope,and these monitoring data were collected and analyzed. 展开更多
关键词 LANDSLIDE FLAC3D Stability analysis Remote real-time monitor
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Transformer real-time reliability model based on operating conditions 被引量:10
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作者 HE Jian CHENG Lin SUN Yuan-zhang 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2007年第3期378-383,共6页
Operational reliability evaluation theory reflects real-time reliability level of power system. The component failure rate varies with operating conditions. The impact of real-time operating conditions such as ambient... Operational reliability evaluation theory reflects real-time reliability level of power system. The component failure rate varies with operating conditions. The impact of real-time operating conditions such as ambient temperature and transformer MVA (megavolt-ampere) loading on transformer insulation life is studied in this paper. The formula of transformer failure rate based on the winding hottest-spot temperature (HST) is given. Thus the real-time reliability model of transformer based on oper- ating conditions is presented. The work is illustrated using the 1979 IEEE Reliability Test System. The changes of operating conditions are simulated by using hourly load curve and temperature curve, so the curves of real-time reliability indices are ob- tained by using operational reliability evaluation. 展开更多
关键词 Operational reliability real-time reliability model TRANSFORMER Winding hottest-pot temperature (HST)
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Delay-dependent stability and added damping of SDOF real-time dynamic hybrid testing 被引量:14
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作者 Chi Fudong Wang Jinting Jin Feng 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2010年第3期425-438,共14页
It is well-recognized that a transfer system response delay that reduces the test stability inevitably exists in real-time dynamic hybrid testing (RTDHT). This paper focuses on the delay-dependent stability and adde... It is well-recognized that a transfer system response delay that reduces the test stability inevitably exists in real-time dynamic hybrid testing (RTDHT). This paper focuses on the delay-dependent stability and added damping of SDOF systems in RTDHT. The exponential delay term is transferred into a rational fraction by the Pad6 approximation, and the delay-dependent stability conditions and instability mechanism of SDOF RTDHT systems are investigated by the root locus technique. First, the stability conditions are discussed separately for the cases of stiffness, mass, and damping experimental substructure. The use of root locus plots shows that the added damping effect and instability mechanism for mass are different from those for stiffness. For the stiffness experimental substructure case, the instability results from the inherent mode because of an obvious negative damping effect of the delay. For the mass case, the delay introduces an equivalent positive damping into the inherent mode, and instability occurs at an added high frequency mode. Then, the compound stability condition is investigated for a general case and the results show that the mass ratio may have both upper and lower limits to remain stable. Finally, a high-emulational virtual shaking table model is built to validate the stability conclusions. 展开更多
关键词 real-time dynamic hybrid testing root locus analysis delay-dependent stability Pade approximation added damping
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Identifying the stability of housekeeping genes to be used for the quantitative real-time PCR normalization in retinal tissue of streptozotocin-induced diabetic rats 被引量:1
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作者 Muhammad Zulfiqah Sadikan Nurul Alimah Abdul Nasir +2 位作者 Mohammad Johari Ibahim Igor Iezhitsa Renu Agarwal 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2024年第5期794-805,共12页
AIM:To investigate the stability of the seven housekeeping genes:beta-actin(ActB),glyceraldehyde-3-phosphate dehydrogenase(GAPDH),18s ribosomal unit 5(18s),cyclophilin A(CycA),hypoxanthine-guanine phosphoribosyl trans... AIM:To investigate the stability of the seven housekeeping genes:beta-actin(ActB),glyceraldehyde-3-phosphate dehydrogenase(GAPDH),18s ribosomal unit 5(18s),cyclophilin A(CycA),hypoxanthine-guanine phosphoribosyl transferase(HPRT),ribosomal protein large P0(36B4)and terminal uridylyl transferase 1(U6)in the diabetic retinal tissue of rat model.METHODS:The expression of these seven genes in rat retinal tissues was determined using real-time quantitative reverse transcription polymerase chain reaction(RT-qPCR)in two groups;normal control rats and streptozotocininduced diabetic rats.The stability analysis of gene expression was investigated using geNorm,NormFinder,BestKeeper,and comparative delta-Ct(ΔCt)algorithms.RESULTS:The 36B4 gene was stably expressed in the retinal tissues of normal control animals;however,it was less stable in diabetic retinas.The 18s gene was expressed consistently in both normal control and diabetic rats’retinal tissue.That this gene was the best reference for data normalisation in RT-qPCR studies that used the retinal tissue of streptozotocin-induced diabetic rats.Furthermore,there was no ideal gene stably expressed for use in all experimental settings.CONCLUSION:Identifying relevant genes is a need for achieving RT-qPCR validity and reliability and must be appropriately achieved based on a specific experimental setting. 展开更多
关键词 housekeeping genes stability real-time reverse transcription polymerase chain reaction retinal tissue streptozotocin-induced diabetic rats
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Stability and accuracy of central difference method for real-time dynamic substructure testing considering mass participation coefficient
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作者 Zheng Lichang Xu Guoshan +3 位作者 Yang Ge Wang Zhen Yang Kaibo Zheng Zhenyun 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2024年第3期625-636,共12页
For real-time dynamic substructure testing(RTDST),the influence of the inertia force of fluid specimens on the stability and accuracy of the integration algorithms has never been investigated.Therefore,this study prop... For real-time dynamic substructure testing(RTDST),the influence of the inertia force of fluid specimens on the stability and accuracy of the integration algorithms has never been investigated.Therefore,this study proposes to investigate the stability and accuracy of the central difference method(CDM)for RTDST considering the specimen mass participation coefficient.First,the theory of the CDM for RTDST is presented.Next,the stability and accuracy of the CDM for RTDST considering the specimen mass participation coefficient are investigated.Finally,numerical simulations and experimental tests are conducted for verifying the effectiveness of the method.The study indicates that the stability of the algorithm is affected by the mass participation coefficient of the specimen,and the stability limit first increases and then decreases as the mass participation coefficient increases.In most cases,the mass participation coefficient will increase the stability limit of the algorithm,but in specific circumstances,the algorithm may lose its stability.The stability and accuracy of the CDM considering the mass participation coefficient are verified by numerical simulations and experimental tests on a three-story frame structure with a tuned liquid damper. 展开更多
关键词 real-time dynamic substructure testing central difference method STability mass participation coefficient tuned liquid damper
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Comparative genomics study between high and low laying goose breeds reveals the important role of ESR1 in laying ability 被引量:1
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作者 Qingyuan Ouyang Hengli Xie +8 位作者 Shenqiang Hu Cong Lan Mingxia Ran Jiwei Hu HuaHe Liang Li Hehe Liu Hao Qu Jiwen Wang 《Journal of Integrative Agriculture》 2025年第3期1167-1180,共14页
The low egg production of goose greatly limits the development of the industry.China possesses the most abundant goose breeds resources.In this study,genome resequencing data of swan goose(Anser cygnoides)and domestic... The low egg production of goose greatly limits the development of the industry.China possesses the most abundant goose breeds resources.In this study,genome resequencing data of swan goose(Anser cygnoides)and domesticated high and low laying goose breeds(Anser cygnoides domestiation)were used to identify key genes related to egg laying ability in geese and verify their functions.Selective sweep analyses revealed 416 genes that were specifically selected during the domestication process from swan geese to high laying geese.Furthermore,SNPs and Indels markers were used in GWAS analyses between high and low laying breed geese.The results showed that RTCB,BPIFC,SYN3,SYNE1,VIP,and ESR1 may be related to the differences in laying ability of geese.Notably,only ESR1 was identified simultaneously by GWAS and selective sweep analysis.The genotype of Indelchr3:54429172,located downstream of ESR1,was confirmed to affect the expression of ESR1 in the ovarian stroma and showed significant correlation with body weight at first egg and laying frequency of geese.CCK-8,EdU,and flow cytometry confirmed that ESR1 can promote the apoptosis of goose pre-hierarchical follicles ganulosa cells(phGCs)and inhibit their proliferation.Combined with transcriptome data,it was found ESR1 involved in the function of goose phGCs may be related to MAPK and TGF-beta signaling pathways.Overall,our study used genomic information from different goose breeds to identify an indel located in the downstream of ESR1 associated with goose laying ability.The main pathways and biological processes of ESR1 involved in the regulation of goose laying ability were identified by cell biology and transcriptomics methods.These results are helpful to further understand the laying ability characteristics of goose and improve the egg production of geese. 展开更多
关键词 GOOSE laying ability GENOME ESR1
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奶牛乳房炎病原体三重Real-time PCR检测方法的建立及应用
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作者 郭思宇 高雅欣 +5 位作者 纪佳豪 李梓豪 刘文扬 徐博 王三毛 李睿文 《动物医学进展》 北大核心 2025年第12期39-44,共6页
为了建立同时检测奶牛临床型乳房炎中肺炎克雷伯菌(Kp)、产色葡萄球菌(Sc)和牛支原体(Mb),基于Kp ZKIR基因、Sc sodA基因和Mb opp D/F基因设计特异性引物,建立三重实时定量荧光PCR方法(real-time PCR)。试验采用在单一real-time PCR检... 为了建立同时检测奶牛临床型乳房炎中肺炎克雷伯菌(Kp)、产色葡萄球菌(Sc)和牛支原体(Mb),基于Kp ZKIR基因、Sc sodA基因和Mb opp D/F基因设计特异性引物,建立三重实时定量荧光PCR方法(real-time PCR)。试验采用在单一real-time PCR检测方法的基础上对三重real-time PCR检测方法进行优化,并确定退火条件为60℃,肺炎克雷伯菌、产色葡萄球菌以及牛支原体上、下游引物浓度为20μmol/L、荧光探针浓度为10μmol/L。结果表明,该方法对标准品pUC57-ZKIR-Kp、pUC57-sodA-Sc、pUC57-opp D/F-Mb最低检测限分别为1.55×10^(2) copies/μL、1.44×10^(2) copies/μL、1.34×10^(2) copies/μL,灵敏度高;仅对Kp、Sc、Mb这3种病原产生荧光曲线,对其他病原无交叉反应,特异性强;其中组内、组间变异系数均小于2%,重复性良好。利用建立的多重real-time PCR对233份临床样品进行检测,Kp、Sc、Mb检出率分别为73.09%、21.97%、6.72%,与单一real-time PCR方法检测结果一致。说明建立的多重real-time PCR在实际应用中具有灵敏度高、特异性强、重复性良好、检测速度快等优点,可为奶牛临床型乳房炎病原的快速检测、临床诊断和流行病学调查提供有效检测手段。 展开更多
关键词 临床型乳房炎 三重real-time PCR 肺炎克雷伯菌 产色葡萄球菌 牛支原体
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An Empirical Study on the Reflective Feedback Model Based on Pedagogical Agents to Improve Students’ Problem-Solving Ability 被引量:1
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作者 Hui-Lin Zhang Jun-Jie Yan +4 位作者 Jing Liu Zhuo-Cen Zou Qun-Fang Zeng Yu-Lu Jin Jian-Nan Wu 《教育技术与创新》 2025年第1期66-75,共10页
This study constructs a reflective feedback model based on a pedagogical agent(PA)and explores its impact on students’problem-solving ability and cognitive load.A quasi-experimental design was used in the study,with ... This study constructs a reflective feedback model based on a pedagogical agent(PA)and explores its impact on students’problem-solving ability and cognitive load.A quasi-experimental design was used in the study,with 84 students from a middle school selected as the research subjects(44 in the experimental group and 40 in the control group).The experimental group used the reflective feedback model,while the control group used the factual feedback model.The results show that,compared with factual feedback,the reflective feedback model based on the pedagogical agent significantly improves students’problem-solving ability,especially at the action and thinking levels.In addition,this model effectively reduces students’cognitive load,especially in terms of internal and external load. 展开更多
关键词 pedagogical agent problem-solving ability reflective feedback
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