One of the most important features of modem minor satellites is to realize autonomous moving. The perfomance of the satellite autonomous computer operating system acting as the control center is of utrnost importance....One of the most important features of modem minor satellites is to realize autonomous moving. The perfomance of the satellite autonomous computer operating system acting as the control center is of utrnost importance. The recent trend in operating system development is adopting microkernel architecture that holds such advantages as microminiaturization, modularity, portability and extendibility. IPC is the key of microkernel design. Message-based IPC mechanism is generally used in existing microkernel Operating system. It is of consistency, safety and reliability.However, it can not provide efficient support for real-time applications in satellite systems and it only applies to loose coupling multi-processor architecture. In this paper, an improvement solution for existing message-based IPC is proposed at first to obtain real-time performance. Then a new IPC mechanism is designed. It particulary applies to shared memory tight coupling multi-processor architecture.展开更多
[Objective] This study aimed to establish a quantitative real-time PCR （qRT-PCR） system for detecting the expression of rice beta-glucosidase gene Os1bglu4.[Method] The PCR was conducted with SYBR Green Ⅰ method,us...[Objective] This study aimed to establish a quantitative real-time PCR （qRT-PCR） system for detecting the expression of rice beta-glucosidase gene Os1bglu4.[Method] The PCR was conducted with SYBR Green Ⅰ method,using the primers of reference gene actin or ubiquitin.[Result] Actin was more suitable to be the reference gene than ubiquitin.More accurate results were obtained when the 100 ng cDNA template was added at a large volume and a lower concentration.The primer concentration in the range from 0.2 to 0.8 μmol/L we set had no significant influence on the results,so,0.4 μmol/L was selected as the optimal primer concentration in this study.The amplification efficiency was greatly reduced when the annealing temperature was set at 64 ℃,therefore,annealing temperature was set at 60 ℃.Compared with the reaction system of 25 μl,the fluorescence intensity was significantly lower but the CT value did not change greatly in 10 μl system.So,the 10 μl reaction system was selected,which significantly reduces the research costs for the detection of a large amount of samples in future study.展开更多
The controller plays an important rol e in microwave landing system(MLS) navigation equipment.The embedded advanced reduced-instruction-set-computing(RISC) machine(ARM) is introduced to de velop a new MLS controller b...The controller plays an important rol e in microwave landing system(MLS) navigation equipment.The embedded advanced reduced-instruction-set-computing(RISC) machine(ARM) is introduced to de velop a new MLS controller based on the functional requirements.A design includ ing hardware and software of an ARM system is given.The hardware design involve s a ARM smallest system and its interface.μC/OS-II,a good real-time operating system(RTOS),is i ntroduced in the software design.The task partitioning and management are also i nvolved.The experimental results demonstrate that the embedded ARM technique is an effective way in the complex multi-task system,and it meets the high inte gration and real-time processing requirements of the navigation equipment.展开更多
文摘One of the most important features of modem minor satellites is to realize autonomous moving. The perfomance of the satellite autonomous computer operating system acting as the control center is of utrnost importance. The recent trend in operating system development is adopting microkernel architecture that holds such advantages as microminiaturization, modularity, portability and extendibility. IPC is the key of microkernel design. Message-based IPC mechanism is generally used in existing microkernel Operating system. It is of consistency, safety and reliability.However, it can not provide efficient support for real-time applications in satellite systems and it only applies to loose coupling multi-processor architecture. In this paper, an improvement solution for existing message-based IPC is proposed at first to obtain real-time performance. Then a new IPC mechanism is designed. It particulary applies to shared memory tight coupling multi-processor architecture.
基金Supported by Guizhou International Cooperation Project on Science and Technology[(2013)7040]the 20th Project of the Joint Committee on Scientific and Technical Cooperation between the Government of the Kingdom of Thailand and the Government of the People’s Republic of China (20-606J)the Fund from Suranaree University of Technology,Thailand (SUT3-304-54-12-29)
文摘[Objective] This study aimed to establish a quantitative real-time PCR （qRT-PCR） system for detecting the expression of rice beta-glucosidase gene Os1bglu4.[Method] The PCR was conducted with SYBR Green Ⅰ method,using the primers of reference gene actin or ubiquitin.[Result] Actin was more suitable to be the reference gene than ubiquitin.More accurate results were obtained when the 100 ng cDNA template was added at a large volume and a lower concentration.The primer concentration in the range from 0.2 to 0.8 μmol/L we set had no significant influence on the results,so,0.4 μmol/L was selected as the optimal primer concentration in this study.The amplification efficiency was greatly reduced when the annealing temperature was set at 64 ℃,therefore,annealing temperature was set at 60 ℃.Compared with the reaction system of 25 μl,the fluorescence intensity was significantly lower but the CT value did not change greatly in 10 μl system.So,the 10 μl reaction system was selected,which significantly reduces the research costs for the detection of a large amount of samples in future study.
文摘The controller plays an important rol e in microwave landing system(MLS) navigation equipment.The embedded advanced reduced-instruction-set-computing(RISC) machine(ARM) is introduced to de velop a new MLS controller based on the functional requirements.A design includ ing hardware and software of an ARM system is given.The hardware design involve s a ARM smallest system and its interface.μC/OS-II,a good real-time operating system(RTOS),is i ntroduced in the software design.The task partitioning and management are also i nvolved.The experimental results demonstrate that the embedded ARM technique is an effective way in the complex multi-task system,and it meets the high inte gration and real-time processing requirements of the navigation equipment.
